Photocatalytic Systems Based on ZnO Produced by Supercritical Antisolvent for Ceftriaxone Degradation

: Emerging contaminants are a signiﬁcant issue in the environment. Photocatalysis is proposed as a solution for the degradation of pollutants contained in wastewater. In this work, ZnO-based photocatalysts have been produced and tested for the photocatalytic degradation of an antibiotic; speciﬁcally, ceftriaxone has been used as a model contaminant. Moreover, there is particular interest in combining small-size ZnO particles and β -cyclodextrin ( β -CD), creating a hybrid photocatalyst. Zinc acetate (ZnAc) (subsequently calcinated into ZnO) and β -CD particles with a mean diameter of 0.086 and 0.38 µ m, respectively, were obtained using the supercritical antisolvent process (SAS). The produced photocatalysts include combinations of commercial and micronized particles of ZnO and β -CD and commercial and micronized ZnO. All the samples were characterized through UV–Vis diffuse reﬂectance spectroscopy (DRS), and the band gap values were calculated. Raman and FT-IR measurements conﬁrmed the presence of ZnO and the existence of functional groups due to the β -cyclodextrin and ZnO combination in the hybrid photocatalysts. Wide-angle X-ray diffraction patterns proved that wurtzite is the main crystalline phase for all hybrid photocatalytic systems. In the photocatalytic degradation tests, it was observed that all the photocatalytic systems exhibited 100% removal efﬁciency within a few minutes. However, the commercial ZnO/micronized β -CD hybrid system is the photocatalyst that shows the best performance; in fact, when using this hybrid system, ceftriaxone was entirely degraded in 1 min.


Introduction
Nowadays, the problem of pollution due to industrial growth is a topic being treated with great interest due to common sense reasoning that human beings must take care of the environment and, consequently, preserve the state of the ecosystems in the best possible way. This work investigated a possible solution to the problem of so-called emerging contaminants (ECs), which are compounds of diverse origins and nature that have the potential to negatively impact the environment and cause adverse effects on human health. These compounds have not been routinely monitored; however, they are currently detected in the environment. Among the emerging contaminants are additives, personal care products, pesticides, flame retardants, and pharmaceuticals. Among the pharmaceutical products most detected in water are anti-inflammatories and analgesics, such as paracetamol, acetylsalicylic acid, ibuprofen, and diclofenac, and antibiotics, such as tetracyclines, macrolides, b-lactams, penicillins, quinolones, sulfonamides, fluoroquinolones, chloramphenicol, and imidazole derivatives. Different studies report the measured concentrations of many pharmaceutical products in the waters of different continents. For example, Ivešić et al. detected concentrations of 1100 ng × L −1 of different types of antibiotics in European waters [1]; Agunbiade and Moodley measured a concentration of 14,331 ng × L −1 for antibiotics in African waters [2][3][4]. chemical splitting of water into hydrogen and oxygen in the presence of TiO 2 [28]. Recently, attention has been paid to the application of photocatalysis to remove organic and inorganic substances in liquid or gaseous systems for environmental remediation, industrial plants, and drinking water. A promising semiconductor is ZnO, proving its effectiveness for degradation reactions in a wide range of compounds and environments, highlighting it as being a low-cost material, and it has also been studied in different morphologies, such as nanoparticles [29,30]. For example, the morphology of photocatalysts can be changed by being micronized.
An innovative and environmentally friendly technique for synthesizing photocatalysts in the form of nanoparticles is supercritical antisolvent (SAS) precipitation [31][32][33]. The SAS micronization technique uses supercritical carbon dioxide (scCO 2 ) as an antisolvent. In addition, to produce nanoparticles of uniform sizes, the SAS technique does not require large amounts of toxic solvents and contaminants, considerably reducing the environmental impact on catalyst preparation. Moreover, the obtained catalyst does not have organic residues; therefore, the purity of the micronized photocatalyst in nanoparticles is high. It is worth noting that the presence of external substances, such as β-cyclodextrins (β-CD) for making up inclusion complexes, could help with improving the performance of a photocatalytic system [34].
The aim of this work is the production of nanoparticles of β-CD and ZnAc obtained by the SAS technique and the preparation of composite photocatalysts for the removal of organic pollutants. Commercial β-CD and ZnO and β-CD and ZnO micronized by SAS were combined to evaluate the best catalytic performances. In this regard, the different samples were compared using ceftriaxone as a model contaminant and a batch-type photoreactor irradiated by UV LED strips to identify any possible improvements to photocatalytic activity regarding contaminant degradation.

Characterization of Micronized Particles
The samples produced by the SAS process were analyzed by field emission scanning electron microscopy (FESEM). An exemplificative image of β-CD microparticles is reported in Figure 1a, whereas the image of the unprocessed β-CD is reported in Figure 1b. Heterogeneous photocatalysis is based on the use of semiconductors (TiO2, ZnO, etc.) as catalysts and the presence of radiation sources [25][26][27]. Great interest in heterogeneous photocatalysis began with Fujishima and Honda when, in 1972, they developed the photochemical splitting of water into hydrogen and oxygen in the presence of TiO2 [28]. Recently, attention has been paid to the application of photocatalysis to remove organic and inorganic substances in liquid or gaseous systems for environmental remediation, industrial plants, and drinking water. A promising semiconductor is ZnO, proving its effectiveness for degradation reactions in a wide range of compounds and environments, highlighting it as being a low-cost material, and it has also been studied in different morphologies, such as nanoparticles [29,30]. For example, the morphology of photocatalysts can be changed by being micronized.
An innovative and environmentally friendly technique for synthesizing photocatalysts in the form of nanoparticles is supercritical antisolvent (SAS) precipitation [31][32][33]. The SAS micronization technique uses supercritical carbon dioxide (scCO2) as an antisolvent. In addition, to produce nanoparticles of uniform sizes, the SAS technique does not require large amounts of toxic solvents and contaminants, considerably reducing the environmental impact on catalyst preparation. Moreover, the obtained catalyst does not have organic residues; therefore, the purity of the micronized photocatalyst in nanoparticles is high. It is worth noting that the presence of external substances, such as β-cyclodextrins (β-CD) for making up inclusion complexes, could help with improving the performance of a photocatalytic system [34].
The aim of this work is the production of nanoparticles of β-CD and ZnAc obtained by the SAS technique and the preparation of composite photocatalysts for the removal of organic pollutants. Commercial β-CD and ZnO and β-CD and ZnO micronized by SAS were combined to evaluate the best catalytic performances. In this regard, the different samples were compared using ceftriaxone as a model contaminant and a batch-type photoreactor irradiated by UV LED strips to identify any possible improvements to photocatalytic activity regarding contaminant degradation.

Characterization of Micronized Particles
The samples produced by the SAS process were analyzed by field emission scanning electron microscopy (FESEM). An exemplificative image of β-CD microparticles is reported in Figure 1a, whereas the image of the unprocessed β-CD is reported in Figure 1b. Through the FESEM images, it is possible to compare the morphologies of the SAS-micronized sample with the unprocessed version; it is evident that particle mor- Through the FESEM images, it is possible to compare the morphologies of the SASmicronized sample with the unprocessed version; it is evident that particle morphology changes significantly after the SAS process. Indeed, unprocessed β-CD constitutes large crystals (Figure 1b), whereas the SAS-precipitated material SAS is in the form of submicrometric particles (Figure 1a). phology changes significantly after the SAS process. Indeed, unprocessed β-CD constitutes large crystals (Figure 1b), whereas the SAS-precipitated material SAS is in the form of sub-micrometric particles (Figure 1a). Figure 2 reports an image of ZnAc microparticles precipitated from dimethylsulfoxide (DMSO) using the SAS process at 150 bar, 40 °C, and 15 mg/mL. Using the Sigma Scan Pro 5.0 software, it was possible to estimate the mean diameter of the SAS-micronized ZnAc and β-CD particles from FESEM images. Figure 3 shows the particle distributions of both materials. In addition, the mean diameter and standard deviation are reported in Table 1. ZnAc powders precipitated by SAS constitute nanoparticles with a mean diameter lower than 100 nm, whereas β-CD precipitated in the form of sub-microparticles had a mean diameter of about 0.4 µm.   Using the Sigma Scan Pro 5.0 software, it was possible to estimate the mean diameter of the SAS-micronized ZnAc and β-CD particles from FESEM images. Figure 3 shows the particle distributions of both materials. In addition, the mean diameter and standard deviation are reported in Table 1. ZnAc powders precipitated by SAS constitute nanoparticles with a mean diameter lower than 100 nm, whereas β-CD precipitated in the form of sub-microparticles had a mean diameter of about 0.4 µm. phology changes significantly after the SAS process. Indeed, unprocessed β-CD constitutes large crystals (Figure 1b), whereas the SAS-precipitated material SAS is in the form of sub-micrometric particles (Figure 1a). Figure 2 reports an image of ZnAc microparticles precipitated from dimethylsulfoxide (DMSO) using the SAS process at 150 bar, 40 °C, and 15 mg/mL. Using the Sigma Scan Pro 5.0 software, it was possible to estimate the mean diameter of the SAS-micronized ZnAc and β-CD particles from FESEM images. Figure 3 shows the particle distributions of both materials. In addition, the mean diameter and standard deviation are reported in Table 1. ZnAc powders precipitated by SAS constitute nanoparticles with a mean diameter lower than 100 nm, whereas β-CD precipitated in the form of sub-microparticles had a mean diameter of about 0.4 µm.    The FESEM image of the hybrid photocatalyst obtained by combining micronized ZnO and micronized β-CD is reported below (Figure 4).

FESEM Image of Micronized Photocatalyst
The FESEM image of the hybrid photocatalyst obtained by combining micronized ZnO and micronized β-CD is reported below (Figure 4).
From this image, it can be seen that the combined photocatalyst has a mostly coalescent appearance; this is probably due to the hybrid sample preparation procedure that involves a drying step at room temperature, which could lead to particle aggregation.

UV-Vis Diffuse Reflectance Spectroscopy (DRS)
The reflectance spectra of the different photocatalysts in the range from 200 to 900 nm of wavelength (λ) are reported in Figure 5. All the samples evidenced remarkable optical absorption in the UV region. It can be observed that the greatest absorption of all samples is in the UV-A region (315-400 nm) [35][36][37]. Through UV-Vis DRS measurements, the band gap (Eg) of the photocatalysts was From this image, it can be seen that the combined photocatalyst has a mostly coalescent appearance; this is probably due to the hybrid sample preparation procedure that involves a drying step at room temperature, which could lead to particle aggregation.

UV-Vis Diffuse Reflectance Spectroscopy (DRS)
The reflectance spectra of the different photocatalysts in the range from 200 to 900 nm of wavelength (λ) are reported in Figure 5.

FESEM Image of Micronized Photocatalyst
The FESEM image of the hybrid photocatalyst obtained by combining micronized ZnO and micronized β-CD is reported below (Figure 4).
From this image, it can be seen that the combined photocatalyst has a mostly coalescent appearance; this is probably due to the hybrid sample preparation procedure that involves a drying step at room temperature, which could lead to particle aggregation.

UV-Vis Diffuse Reflectance Spectroscopy (DRS)
The reflectance spectra of the different photocatalysts in the range from 200 to 900 nm of wavelength (λ) are reported in Figure 5. All the samples evidenced remarkable optical absorption in the UV region. It can be observed that the greatest absorption of all samples is in the UV-A region (315-400 nm) [35][36][37]. Through UV-Vis DRS measurements, the band gap (Eg) of the photocatalysts was All the samples evidenced remarkable optical absorption in the UV region. It can be observed that the greatest absorption of all samples is in the UV-A region (315-400 nm) [35][36][37]. Through UV-Vis DRS measurements, the band gap (E g ) of the photocatalysts was obtained by the Kubelka-Munk function. The band gap values of all photocatalysts calculated from the intersection of a tangent line to the resulting curve with the x-axis ( Figure 6) are reported in Table 2  The observed band gap values for all SAS-micronized samples are slightly lower than that of commercial ZnO (3.22 eV), according to the Eg value reported in the literature (Eg = 3.37 eV) [38]. The lowest band gap energy corresponds to the value observed for the hybrid photocatalyst constituted by micronized ZnO/micronized β-CD (3.12 eV).

Specific Surface Area (SSA)
The specific surface area of all photocatalyst configurations was estimated through the BET equation. The results are reported in Table 2. The degasification pretreatment was performed at 50 °C for 120 min. The BET surface area values are similar to those reported in the literature [37,39]. A higher specific surface area can be observed in samples with SAS-micronized ZnO.  Micronized ZnO/Micronized β-cyclodextrins 3.12 28 32 The observed band gap values for all SAS-micronized samples are slightly lower than that of commercial ZnO (3.22 eV), according to the E g value reported in the literature (E g = 3.37 eV) [38]. The lowest band gap energy corresponds to the value observed for the hybrid photocatalyst constituted by micronized ZnO/micronized β-CD (3.12 eV).

Specific Surface Area (SSA)
The specific surface area of all photocatalyst configurations was estimated through the BET equation. The results are reported in Table 2. The degasification pretreatment was performed at 50 • C for 120 min. The BET surface area values are similar to those reported in the literature [37,39]. A higher specific surface area can be observed in samples with SAS-micronized ZnO. The FT-IR analysis was performed on all the photocatalysts configurations, commercial β-cyclodextrin, and micronized β-cyclodextrin. The results of the FT-IR tests are reported in Figure 7.

Fourier-Transform Infrared Spectroscopy (FT-IR)
The FT-IR analysis was performed on all the photocatalysts configurations, commercial β-cyclodextrin, and micronized β-cyclodextrin. The results of the FT-IR tests are reported in Figure 7. Commercial ZnO and micronized ZnO show similar bands with sightly differences, which implies that ZnO is present, mainly referring to the so-called "fingerprint region" that spans from 1500 cm −1 to 500 cm −1 , where absorptions for the chemical compounds or molecules are found [40]. All samples containing ZnO show the characteristic band around 540 cm −1 , attributed to the bond between Zn and O [41,42]. There are similar bands in the hybrid photocatalyst configurations, especially from 1800 cm −1 to 1200 cm −1 . For instance, it is possible to observe bands at around 1572 cm −1 and 1420 cm −1 (shifted from 1383 cm −1 ) in all three samples that could correspond to the -C=C-group and bending vibration of the -CH group of the ZnO/β-CD combination [36,43]. In addition, in the commercial ZnO/commercial β-CD configuration, there are two bands at 880 cm −1 and 1013 cm −1 , which can be attributed to -C-O-C functional groups and -O-bonds. The band that appears around 2350 cm −1 in all samples might be related to the possible absorption of CO2 on the surface of the samples, as the measurements were conducted at standard atmospheric conditions [44]. In the case of the β-CD samples, there are some characteristic bands at 1155 cm −1 and 2924 cm −1 that are attributed to the stretching frequency of the C-C bond and C-H bond, respectively [45]. Commercial ZnO and micronized ZnO show similar bands with sightly differences, which implies that ZnO is present, mainly referring to the so-called "fingerprint region", which spans from 1500 cm −1 to 500 cm −1 , which is where absorptions for the chemical compounds or molecules Commercial ZnO and micronized ZnO show similar bands with sightly differences, which implies that ZnO is present, mainly referring to the so-called "fingerprint region" that spans from 1500 cm −1 to 500 cm −1 , where absorptions for the chemical compounds or molecules are found [40]. All samples containing ZnO show the characteristic band around 540 cm −1 , attributed to the bond between Zn and O [41,42]. There are similar bands in the hybrid photocatalyst configurations, especially from 1800 cm −1 to 1200 cm −1 . For instance, it is possible to observe bands at around 1572 cm −1 and 1420 cm −1 (shifted from 1383 cm −1 ) in all three samples that could correspond to the -C=C-group and bending vibration of the -CH group of the ZnO/β-CD combination [36,43]. In addition, in the commercial ZnO/commercial β-CD configuration, there are two bands at 880 cm −1 and 1013 cm −1 , which can be attributed to -C-O-C functional groups and -O-bonds. The band that appears around 2350 cm −1 in all samples might be related to the possible absorption of CO 2 on the surface of the samples, as the measurements were conducted at standard atmospheric conditions [44]. In the case of the β-CD samples, there are some characteristic bands at 1155 cm −1 and 2924 cm −1 that are attributed to the stretching frequency of the C-C bond and C-H bond, respectively [45]. Commercial ZnO and micronized ZnO show similar bands with sightly differences, which implies that ZnO is present, mainly referring to the so-called "fingerprint region", which spans from 1500 cm −1 to 500 cm −1 , which is where absorptions for the chemical compounds or molecules are found [40]. All samples containing ZnO show the characteristic band around 540 cm −1 , attributed to the bond between Zn and O [41,42]. There are similar bands in the hybrid photocatalyst configurations, especially from 1800 cm −1 to 1200 cm −1 . For instance, it is possible to observe peaks at around 1572 cm −1 and 1420 cm −1 (shifted from 1383 cm −1 ) in all three samples that could correspond to the -C=C-group and bending vibration of the -CH group of the ZnO/β-CD combination [36,43]. In addition, in the commercial ZnO/commercial β-CD configuration, there are two bands, at 880 cm −1 and 1013 cm −1 , which can be attributed to -C-O-C functional groups and -O-bonds. The band that appears around 2350 cm −1 in all samples might be related to the possible absorption of CO 2 on the surface of the samples, as the measurements were conducted at standard atmospheric conditions [44].  (1 0 2) crystalline planes, respectively, which are associated with the wurtzite hexagonal crystal structure [46]. The X-ray diffraction studies underline the fact that the combination of a commercial or SAS-micronized ZnO photocatalyst with commercial β-CD or β-CD precipitated by SAS in the hybrid materials does not cause any modification to the crystalline structure because the typical signals of the wurtzite phase are evident, which is in agreement with the reported JCPDS data [46,47]. No characteristic peaks were observed other than ZnO, confirming that the main crystalline phase is wurtzite without detecting the appearance of other diffraction peaks correlated to β-CD [48]. The Debye-Scherrer formula [49] was used for the determination of crystallite size (Table 2), considering the main WAXD peak of ZnO corresponding to the (1 0 0) crystalline plane [50]. Among all the hybrid photocatalytic systems, the commercial ZnO/micronized β-CD material exhibits the highest value for crystallite size (41 nm) [46].

Raman Spectroscopy
Raman spectroscopy was performed on all photocatalytic systems, and additionally, it was also performed on commercial β-CD, from 100 cm −1 to 800 cm −1 (Raman shift) at room temperature. The Raman spectra for the different photocatalytic formulations are reported in Figure 9.

Raman Spectroscopy
Raman spectroscopy was performed on all photocatalytic systems, and additionally, it was also performed on commercial β-CD, from 100 cm −1 to 800 cm −1 (Raman shift) at room temperature. The Raman spectra for the different photocatalytic formulations are reported in Figure 9. As reported in the literature, it is also possible to observe the characteristic Raman bands related to ZnO in all the samples containing β-CD [51,52]. The signals are located at 437 cm −1 , 381 cm −1 , 332 cm −1 , and 583 cm −1 and correspond to E2(high), A1(TO), 2E2(M), and E(LO) phonon modes, respectively. These are described as the Raman-active modes of the ZnO wurtzite crystal.

Photocatalytic Activity Results
The photocatalysts used for the activity tests are:   As reported in the literature, it is also possible to observe the characteristic Raman bands related to ZnO in all the samples containing β-CD [51,52]. The signals are located at 437 cm −1 , 381 cm −1 , 332 cm −1 , and 583 cm −1 and correspond to E 2 (high), A 1 (TO), 2E 2 (M), and E(LO) phonon modes, respectively. These are described as the Raman-active modes of the ZnO wurtzite crystal.

Photocatalytic Activity Results
The photocatalysts used for the activity tests are: Micronized ZnO/micronized β-CD combination. Figure 10 shows the results of the photolysis process on the ceftriaxone solution without a photocatalyst and the degradation of ceftriaxone under UV radiation in the presence of the formulated photocatalysts.
The ceftriaxone-relative concentration after 15 min of UV light without a photocatalyst decreased by up to 10%, underlining a negligible photolysis effect. On the other hand, the presence of photocatalytic systems allowed for the removal of the contaminant in a short time when compared with the photolysis test. All the hybrid photocatalysts show excellent performance in terms of removing ceftriaxone when compared with the literature, where a treatment time of approximately 120 min was shown [53,54]. Notably, under 1 min of UV light, the removal efficiency is 83% for the sole commercial ZnO, 31% for the SAS-micronized ZnO, 78% for the commercial ZnO/commercial β-CD, 62% for micronized ZnO/micronized β-CD, whereas complete ceftriaxone degradation was achieved when using commercial ZnO/micronized β-CD, underlining that the latter is the best photocatalyst. The ceftriaxone-relative concentration after 15 min of UV light without a photocatalyst decreased by up to 10%, underlining a negligible photolysis effect. On the other hand, the presence of photocatalytic systems allowed for the removal of the contaminant in a short time when compared with the photolysis test. All the hybrid photocatalysts show excellent performance in terms of removing ceftriaxone when compared with the literature, where a treatment time of approximately 120 min was shown [53,54]. Notably, under 1 min of UV light, the removal efficiency is 83% for the sole commercial ZnO, 31% for the SAS-micronized ZnO, 78% for the commercial ZnO/commercial β-CD, 62% for micronized ZnO/micronized β-CD, whereas complete ceftriaxone degradation was achieved when using commercial ZnO/micronized β-CD, underlining that the latter is the best photocatalyst.
In addition, the kinetics of ceftriaxone degradation were evaluated by considering a first-order reaction, as reported in the literature for ZnO-based photocatalysts [35,36,55]. The kinetic results are reported in Figure 11. In addition, the kinetics of ceftriaxone degradation were evaluated by considering a first-order reaction, as reported in the literature for ZnO-based photocatalysts [35,36,55]. The kinetic results are reported in Figure 11.
The higher apparent kinetic degradation constant was recorded in the presence of the commercial ZnO/micronized β-CD system, and the lower value for the sole, micronized ZnO. The hybrid photocatalytic system with micronized β-CD showed a better performance than ZnO only, which is in agreement with the literature [43,45]. This result implies an improvement in the photocatalytic degradation of organic compounds by forming the inclusion complex with cyclodextrins, which helps the overall reaction mechanism of contaminant degradation [56,57]. The hybrid photocatalytic system with micronized β-CD showed better performance than ZnO only, which is in agreement with the literature [43,45]. This result implies an improvement in the photocatalytic performances because the β-CD can encapsulate the ceftriaxone, leading to the formation of inclusion complexes that increase the contact between the guest antibiotic molecules and the catalyst surface [56,57]. In more detail, the presence of β-CD allows for the capturing of the ceftriaxone inside its hydrophobic cavity by generating inclusion complexes, thereby increasing the contact between the antibiotic adsorbed on the catalyst surface and the reactive oxygen species created from UV light irradiation. Then, the photogenerated reactive oxygen species react with ceftriaxone molecules, enhancing photocatalytic degradation performance. The higher apparent kinetic degradation constant was recorded in the presence of the commercial ZnO/micronized β-CD system, and the lower value for the sole, micronized ZnO. The hybrid photocatalytic system with micronized β-CD showed a better performance than ZnO only, which is in agreement with the literature [43,45]. This result implies an improvement in the photocatalytic degradation of organic compounds by forming the inclusion complex with cyclodextrins, which helps the overall reaction mechanism of contaminant degradation [56,57]. The hybrid photocatalytic system with micronized β-CD showed better performance than ZnO only, which is in agreement with the literature [43,45]. This result implies an improvement in the photocatalytic performances because the β-CD can encapsulate the ceftriaxone, leading to the formation of inclusion complexes that increase the contact between the guest antibiotic molecules and the catalyst surface [56,57]. In more detail, the presence of β-CD allows for the capturing of the ceftriaxone inside its hydrophobic cavity by generating inclusion complexes, thereby increasing the contact between the antibiotic adsorbed on the catalyst surface and the reactive oxygen species created from UV light irradiation. Then, the photogenerated reactive oxygen species react with ceftriaxone molecules, enhancing photocatalytic degradation performance.
In order to explain the different photocatalytic activity observed for the tested photocatalysts, the amount of ceftriaxone adsorbed in dark conditions was analyzed and compared to the calculated K values (Table 3). Table 3. The amount of ceftriaxone adsorbed according to photocatalyst dosage and the apparent degradation kinetic constant obtained for the used photocatalytic systems; the determination coefficient (R 2 ) for the pseudo-first-order kinetics is also reported. In order to explain the different photocatalytic activity observed for the tested photocatalysts, the amount of ceftriaxone adsorbed in dark conditions was analyzed and compared to the calculated K values (Table 3). Table 3. The amount of ceftriaxone adsorbed according to photocatalyst dosage and the apparent degradation kinetic constant obtained for the used photocatalytic systems; the determination coefficient (R 2 ) for the pseudo-first-order kinetics is also reported.

Photocatalyst
Adsorbed Ceftriaxone, mg ceftriaxone /mg catalyst × 10 3 K, min −1 R 2 The obtained values are reported as a function of the apparent kinetic constant values ( Figure 12). The K values increased linearly with the amount of ceftriaxone adsorbed (Table 3). Therefore, the photocatalytic activity is strictly correlated to the adsorption capacity of the formulated catalyst toward the pollutant to be removed.
The commercial ZnO/micronized β-CD photocatalyst was recovered from the treated aqueous solution and used five times without significant variation in the pollutant degradation kinetic constant (Figure 13), confirming the stability of the catalytic material. The obtained values are reported as a function of the apparent kinetic constant values ( Figure 12). The K values increased linearly with the amount of ceftriaxone adsorbed (Table 3). Therefore, the photocatalytic activity is strictly correlated to the adsorption capacity of the formulated catalyst toward the pollutant to be removed. The commercial ZnO/micronized β-CD photocatalyst was recovered from the treated aqueous solution and used five times without significant variation in the pollutant degradation kinetic constant (Figure 13), confirming the stability of the catalytic material.

Micronization of ZnAc and β-CD by Supercritical Antisolvent (SAS) Process and Preparation of ZnO/β-CD Photocatalyst
ZnAc and β-CD powders were micronized by the SAS process. The SAS plant is composed of a cylindrical vessel with an internal volume equal to 500 cm 3 , which is the center of the process. To reach the desired pressure and feed the CO 2 (antisolvent) and liquid solution (DMSO + solute) into the chamber, two high-pressure pumps were employed. CO 2 is pre-cooled using a refrigerating bath before sending it into the chamber, while the liquid solution is injected into the precipitation chamber through a stainless-steel nozzle. A proportional integral derivative controller connected with heating bands ensures the operating temperature inside the chamber, whereas a micrometric valve is used for pressure regulation and a test gauge manometer for the pressure measurement. At the bottom of the precipitation chamber, a porous filter, characterized by pores with a diameter of 0.1 µm, is located; it permits the CO 2 -solvent mixture to pass through and allows the collection of the precipitated powders. A rotameter measures the flow rate of CO 2 . At the beginning of each SAS experiment, CO 2 is pumped inside the precipitation chamber until the desired pressure and temperature are reached. When the quasi-steady state composition of solvent and antisolvent within the vessel is achieved, the liquid solution is fed through the nozzle to lead to the micronization of the solute. At the end of the solution injection, CO 2 continues to flow for a calculated time until the complete elimination of the solvent residues. After this washing step, the CO 2 pump is switched off, and the vessel is slowly depressurized to atmospheric pressure. In detail, the liquid flow rates used for the SAS experiments were equal to 1 mL min −1 , and the CO 2 flow rates were equal to 30 g min −1 for both ZnAc and β-CD. Different conditions, in terms of pressure, temperature, and concentration, were used for the micronization of the different compounds. In particular, in this work, for the SAS micronization of β-CD experiments, the conditions optimized in previous work [58] were used, i.e., a temperature equal to 40 • C, a pressure of 150 bar, and a total concentration of 50 mg × mL −1 . For the micronization of ZnAc, the chosen conditions were a pressure equal to 150 bar, a temperature of 40 • C, and a concentration equal to 15 mg × mL −1 . Before each experiment, the first step was preparing the solution; DMSO was used as the solvent, and the right amount of solute was dissolved in 100 mL of DMSO to achieve the desired concentration every time. The powder was recovered in the precipitator at the end of each SAS experiment, whereas the DMSO was extracted by supercritical carbon dioxide (scCO 2 ). The power obtained at the end of the experiment was collected, characterized, and used in the activity test.
To obtain a ZnO/β-CD hybrid photocatalyst system, 4 g/L of ZnO and 3.4 g/L of β-cyclodextrin aqueous solutions were prepared and agitated for 3 h, as optimized in a previous study [58]. During the agitation process, the solution's pH was measured; the optimal pH value is between 5 and 7, which favors the interaction between β-CD and ZnO.
A solution of a known NaOH concentration was added to the suspension to obtain the desired pH value. After that, the obtained suspension was centrifuged, and the solid and liquid phases were separated. The solid phase was carefully washed three times using distilled water. The collected photocatalyst hybrid particles were then dried at room temperature for 24 h. By using this technique, the following hybrid photocatalyst configurations were processed: The amount of ZnO, with respect to β-CD, contained in each hybrid system is about 54%.

Characterization Methods
The sample aspect from a morphological point of view was observed through a field emission scanning electron microscope (FESEM, mod. LEO 1525, Carl Zeiss SMT AG, Oberkochen, Germany); the powders collected in the precipitation chamber for each test were previously dispersed on a carbon tab and subsequently covered with gold-palladium (layer thickness 250 Å) using a sputter coater (mod. 108 A, Agar scientific, Monterotondo, Italy).
The mean diameters and the particle size distributions of the processed powders, considering about 1000 particles for each sample, were calculated with the aid of Sigma Scan Pro image analysis software v5.0 (Aspire Software International, Ashburn, VA, USA).
Fourier transforms infrared (FT-IR) spectra were acquired using an M2000 FT-IR (MI-DAC Co, Costa Mesa, CA, USA) at a resolution of 0.5 cm −1 . A total of 100 mg of KBr powder was well mixed with 1 mg of sample to obtain an infrared transparent matrix. The discs were prepared by compressing the powders through a hydraulic press. The scan wavenumber range was 4000-500 cm −1 , and 16 scan signals were averaged to reduce the noise.
The Brunauer, Emmett, and Teller (BET) surface area of the photocatalysts were obtained through dynamic N 2 adsorption measurement at −196 • C by means of a Costech Sorptometer 1042 (Costech International S.p.A., Milan, Italy) instrument; all the samples were pretreated at 50 • C for 120 min in He flow before the SSA measurements.
A Bruker D8 Advance diffractometer (VANTEC-1 detector), which uses nickel-filtered Cu-Kα radiation, was employed for obtaining wide-angle X-ray diffraction (WAXD) patterns of the prepared photocatalysts. Raman spectra in the range 100-800 cm −1 were obtained using a Dispersive MicroRaman spectrometer (Invia, Renishaw) with a monochromatic laser emitting at 514 nm.
A high-performance liquid chromatographer (HPLC) was utilized to analyze the pollutant concentration at different times of the photocatalytic tests. The technique was developed utilizing a C18 (Agilent Technologies, Santa Clara, CA, USA) as a stationary phase column, working on an isocratic elution using a mobile phase constituted by a mixture of 80:20, KH 2 PO 4 buffer, and methanol, respectively, at a flow rate equal to 0.700 mL × min −1 ; the detection was controlled at wavelengths of 241 nm [59]. The HPLC system corresponds to the Agilent Technologies 1200 infinity series. The HPLC measures the area corresponding to the peak of the characteristic time of the chemical compound, in this case, 2.7 min for ceftriaxone. Previously, it was necessary to make a calibration process and measure a calibration curve in which the scope was utilized to get the concentration of the solution.

Procedure for the Photocatalytic Activity Tests
The photocatalytic activity tests aim to remove the antibiotic present in a liquid effluent. In this specific case, the antibiotic to be utilized is ceftriaxone in an aqueous solution in the presence of the ZnO photocatalyst under UV radiation. The experiments were carried out using 0.15 g of catalyst in 100 mL (photocatalyst dosage: 1.5 g × L −1 ) of ceftriaxone aqueous solutions (initial ceftriaxone concentration = 5 mg × L −1 [60]). The total duration of the tests was 75 min: 60 min in dark phase and 15 min under UV light. The solution was inserted into a cylindrical pyrex reactor (ID = 3 cm and V TOT = 200 mL, Microglass Heim Srl, Naples, Italy); UV-LEDs (nominal power: 12 W; emission centered at 365 nm; irradiance: 13 W/m 2 , provided by LEDlightinghut, Shenzen, China) strip were wrapped around the outer surface of the reactor to enlighten the solution volume. The reactor was also equipped with a device for air distribution to guarantee the presence of the oxygen needed for the reaction. Some samples were taken during the tests at fixed times; the first sample was taken from the reactor and represented the sample at time zero. After that, the photocatalyst was put into the photoreactor. Then, the dark phase began (to leave the system in darkness) and lasted an hour. It was expected that ceftriaxone would be absorbed on the photocatalyst's surface during this time. After the dark phase, the UV-LEDs were turned on, and the photoreaction occurred. The samples were taken at increasing time intervals (until 15 min) from the switching on of the UV LEDs. The samples were taken using a syringe with a volume of 3 mL, which were then filtered and placed in 2 mL vials. The samples were analyzed with an HPLC (High-Performance Liquid Chromatograph) (Agilent Technologies 1200 infinity series, Santa Clara, CA, USA), where the concentration of the sample was measured through the area around the retention time corresponding to ceftriaxone.
The removal efficiency for ceftriaxone is calculated utilizing the following formula: The ceftriaxone degradation follows the pseudo-first-order kinetics described by the subsequent equation:

−ln
The kinetics constant, K, value was estimated from the slope of the straight-line by plotting −ln(C/C 0 ) vs. the irradiation time.

Conclusions
In this work, different hybrid ZnO/β-CD photocatalysts were prepared and subsequently tested for the photocatalytic degradation of ceftriaxone. In particular, the SAS technique allowed for the production of nanoparticulate ZnAc and sub-microparticulate β-CD. All the prepared photocatalytic systems were characterized using different analysis techniques; FESEM microscopy showed the morphology obtained both for ZnAc and for β-CD micronized with supercritical antisolvent (SAS). All hybrid photocatalysts showed the presence of ZnO, demonstrated by Raman and FT-IR spectroscopic analysis. The WAXD study verified that the combination of β-cyclodextrins with a ZnO photocatalyst did not alter the crystalline structure of the semiconductor that is substantially characterized by a wurtzite hexagonal phase. The catalytic activity tests under UV light showed that all photocatalytic systems degraded ceftriaxone in a very short treatment time. The commercial ZnO/micronized β-cyclodextrins system was the photocatalyst with the highest degradation performance because of its higher ceftriaxone adsorption ability in the dark phase when compared to the other tested photocatalysts. Therefore, ZnO/micronized β-CD showed the highest degradation rate of ceftriaxone under UV light, with an apparent kinetics constant equal to 3.21 min −1 , a value that was about two times superior to that obtained using commercial ZnO, which was 1.75 min −1 . Data Availability Statement: Data will be made available upon request.

Conflicts of Interest:
The authors declare no conflict of interest.