Disinfection and Photocatalytic Degradation of Organic Contaminants Using Visible Light-Activated GCN/Ag 2 CrO 4 Nanocomposites

: Visible-light-driven photocatalysts have gained increasing attention in the past few decades in treating emerging contaminants in water and wastewater. In this work, the photocatalytic activity of the coupled graphitic carbon nitride (GCN) and silver chromate (Ag 2 CrO 4 ), herein denoted as GCN/Ag 2 CrO 4 , nanocomposites was evaluated for degrading organic pollutants and inactivating microorganisms under visible light irradiation using a royal blue light-emitting diode (LED). The organic pollutants studied were 2,4-dichlorophenoxyacetic acid (2,4-D) and methyl chlorophenoxy propionic acid (MCPP or Mecoprop-P) present in Killex R , a commercially available herbicide, bovine serum albumin (BSA) protein, and SARS-CoV-2 spike protein. The disinfection experiments were conducted on wastewater secondary efﬂuent. The results showed that over 85% degradation was achieved for both 2,4-D and Mecoprop-P in 120 min while 100% of BSA protein and 77.5% of SARS-CoV-2 protein were degraded in 20 min and 30 min, respectively. Additionally, GCN/Ag 2 CrO 4 nanocomposites led to over one log reduction of cellular ATP ( cATP ), total coliforms, and E. coli in wastewater treatment plant (WWTP) secondary efﬂuent after 60 min of royal blue LED irradiation. It was observed that the degradation performance of a photocatalyst under light irradiation is contaminant-speciﬁc. The binding afﬁnity of the released metal ions from GCN/Ag 2 CrO 4 with protein and ATP functional groups was responsible for the degradation of proteins and the reduction of cATP , while the generated ROS was responsible for the disinfection of total coliforms and E. coli . Overall, the results indicate that GCN/Ag 2 CrO 4 nanocomposite is a promising photocatalyst in degrading organic pollutants and disinfecting microorganisms under visible light irradiation within a reasonable time.


Introduction
Semiconductor photocatalysis is fast emerging as the advanced oxidation process to address environmental pollution and global warming such as organic pollutant removal, disinfection of microorganisms, hydrogen evolution, and CO 2 reduction [1][2][3][4][5]. In semiconductor photocatalysis, photoexcited electrons and holes are generated when the energy absorbed from light is greater than or equal to the bandgap energy of the photocatalysts. These electrons react with the dissolved oxygen in the solution while the holes react with water molecules to produce superoxide radical (O 2 − ) and hydroxyl radical (•OH), respectively. These radicals are referred to as reaction oxidative species (ROS) and they can a potential pathogen and its presence in the water body can indicate the presence of other waterborne pathogens such as bacteria, viruses, and parasites [40].
Photocatalytic degradation of 2,4-D and Killex using TiO 2 has been widely reported [41][42][43]. However, only a few studies focused on the photocatalytic degradation of 2,4-D using GCN. The degradation of 2,4-D using GCN-based composite under natural sunlight showed over 90% removal after 330 min [44]. About 100% degradation of a very small concentration (50 µg/L) of Mecoprop-P was achieved in 7 min using a GCN-based catalyst under simulated solar irradiation [45]. Heydari et al. degraded Killex R (2,4-D and Mecoprop-P) using TiO 2 under natural sunlight exposure with 97% degradation of 2,4-D after 15 days and 100% degradation of Mecoprop-P after 22 days [46]. The enhancement of the photocatalytic activity of GCN in degrading methylene blue (MB) was achieved with its combination with Co-NiS and sulfur to form 1D/2D Co-NiS/S-g-C 3 N 4 heterojunction with 98% degradation in 32 min [47]. ZnFe 2 O 4 /S-g-C 3 N 4 heterojunction was synthesized with varying wt% of S-g-C 3 N 4 . The ZnFe 2 O 4 /S-g-C 3 N 4 (50%) showed the maximum MB degradation under solar irradiation in 150 min [48]. The interaction between BSA and nanoparticles and the structural change to BSA have been investigated with nanoparticles such as MnO 2 [49], CuS [50], and silver nanoparticles (AgNP) [51,52] at different concentrations and compositions. The results show increasing interactions and structural changes to BSA with increasing concentration of the nanoparticles. The disinfection of E. coli using GCN-based composite under a xenon lamp as a solar simulator showed complete inactivation (6.5 log) after 60 min [29]. In the published literature, the focus was on either natural sunlight or solar simulator as broadband light source for the photocatalytic degradation and disinfection. To the best of our knowledge, the combination of a narrowband low-energy visible light source (in this case royal blue LED with a peak wavelength of 460 nm) and GCN-based nanocomposites has not been reported on degrading herbicides and protein and disinfecting WWTP secondary effluent. The benefits of narrowband light are its consistency, directionality (narrow and focused beam), and narrow frequency range [53]. The photons in narrowband light move at the same wavelength (consistency) and in the same direction (directionality) resulting in high light intensity.
This work aims to address the knowledge gaps in evaluating the photocatalytic performance of GCN/Ag 2 CrO 4 nanocomposite under a narrowband low-energy visible light source (royal blue LED) in degrading different organic pollutants such as Killex R (2,4-D and MCPP), BSA protein, SARS-CoV-2 spike protein, and disinfecting WWTP secondary effluent. The reaction kinetics of 2,4-D, Mecoprop-P, and BSA protein were also investigated. The interaction between BSA and the photocatalysts, and the structural changes to BSA were studied using a UV-Vis spectrophotometer; log reduction of cellular ATP (cATP), total coliforms, and E. coli in WWTP secondary effluent was used to determine the efficiency of the catalyst in wastewater disinfection.

Photocatalytic Degradation of Killex R
The dark adsorption of 2,4-D and Mecoprop-P on the synthesized photocatalyst are presented in Figure 1. It is obvious that GCN alone did not adsorb and thus there was no decrease in the concentration of 2,4-D or Mecoprop-P during the dark experiment. Equilibrium was reached within 30 min for Ag 2 CrO 4 and GCN/Ag 2 CrO 4 . A total of 8.06% of 2,4-D was adsorbed on Ag 2 CrO 4 while 9.32% of Mecoprop-P was adsorbed on Ag 2 CrO 4. A total of 6.24% of 2,4-D was adsorbed on GCN/Ag 2 CrO 4 while 15.32% of Mecoprop-P was adsorbed on GCN/Ag 2 CrO 4 . The photocatalytic degradation of GCN/Ag 2 CrO 4 on the 2,4-D and Mecoprop-P in Killex R was investigated and the results are presented in Figure 2. In the control experiment, 2,4-D and Mecoprop-P did not decrease with irradiation by royal blue LED, indicating that the royal blue light (peak wavelength = 460 nm) did not degrade 2,4-D or Mecoprop-P. Using GCN, 16.85% of 2,4-D and 10.06% of Mecoprop-P degraded after 120 min. Ag 2 CrO 4 remains largely unchanged under royal blue LED irradiation when compared to the dark experiment results. It was 7.05% for 2,4-D and 9.19% for Mecoprop- P. This is because silver-based photocatalysts are prone to photolysis and cannot stand long-run duration of photocatalytic degradation [30], and the low surface area of Ag 2 CrO 4 (1.32 m 2 /g) [21] available for the adsorption of 2,4-D and Mecoprop-P and the eventual photocatalytic activity. Silver-based particles have been reported to aggregate [54] while GCN in the GCN/Ag 2 CrO 4 nanocomposite serves to prevent the aggregation of Ag 2 CrO 4 . GCN/Ag 2 CrO 4 showed the highest percent degradation for both 2,4-D and Mecoprop-P at 86.32% and 86.39% in 120 min. area of Ag2CrO4 (1.32 m 2 /g) [21] available for the adsorption of 2,4-D and Mecoprop-P and the eventual photocatalytic activity. Silver-based particles have been reported to aggregate [54] while GCN in the GCN/Ag2CrO4 nanocomposite serves to prevent the aggregation of Ag2CrO4. GCN/Ag2CrO4 showed the highest percent degradation for both 2,4-D and Mecoprop-P at 86.32% and 86.39% in 120 min.
The first-order rate was calculated to evaluate the performance of the different photocatalysts. The rate constant (k) values are summarized in Table 1. For 2,4-D, the k values of GCN and GCN/Ag2CrO4 were 0.16 × 10 −2 min −1 and 1.59 × 10 −2 min −1 respectively. The highest k value for degradation using GCN/Ag2CrO4 was 9.9 times that with GCN alone. For Mecoprop-P, the k values of GCN and GCN/Ag2CrO4 were 0.11 × 10 −2 min −1 and 1.79 × 10 −2 min −1 respectively. The highest k value for the photocatalyst GCN/Ag2CrO4 was 1.79 × 10 −2 min −1 which was 16.3 times that of GCN. This result shows that the deposition of Ag2CrO4 on GCN enhanced its photocatalytic activity by serving as an electron trap for photogenerated electrons in GCN.   area of Ag2CrO4 (1.32 m 2 /g) [21] available for the adsorption of 2,4-D and Mecoprop-P and the eventual photocatalytic activity. Silver-based particles have been reported to aggregate [54] while GCN in the GCN/Ag2CrO4 nanocomposite serves to prevent the aggregation of Ag2CrO4. GCN/Ag2CrO4 showed the highest percent degradation for both 2,4-D and Mecoprop-P at 86.32% and 86.39% in 120 min.
The first-order rate was calculated to evaluate the performance of the different photocatalysts. The rate constant (k) values are summarized in Table 1. For 2,4-D, the k values of GCN and GCN/Ag2CrO4 were 0.16 × 10 −2 min −1 and 1.59 × 10 −2 min −1 respectively. The highest k value for degradation using GCN/Ag2CrO4 was 9.9 times that with GCN alone. For Mecoprop-P, the k values of GCN and GCN/Ag2CrO4 were 0.11 × 10 −2 min −1 and 1.79 × 10 −2 min −1 respectively. The highest k value for the photocatalyst GCN/Ag2CrO4 was 1.79 × 10 −2 min −1 which was 16.3 times that of GCN. This result shows that the deposition of Ag2CrO4 on GCN enhanced its photocatalytic activity by serving as an electron trap for photogenerated electrons in GCN.   The first-order rate was calculated to evaluate the performance of the different photocatalysts. The rate constant (k) values are summarized in Table 1. For 2,4-D, the k values of GCN and GCN/Ag 2 CrO 4 were 0.16 × 10 −2 min −1 and 1.59 × 10 −2 min −1 respectively. The highest k value for degradation using GCN/Ag 2 CrO 4 was 9.9 times that with GCN alone. For Mecoprop-P, the k values of GCN and GCN/Ag 2 CrO 4 were 0.11 × 10 −2 min −1 and 1.79 × 10 −2 min −1 respectively. The highest k value for the photocatalyst GCN/Ag 2 CrO 4 was 1.79 × 10 −2 min −1 which was 16.3 times that of GCN. This result shows that the deposition of Ag 2 CrO 4 on GCN enhanced its photocatalytic activity by serving as an electron trap for photogenerated electrons in GCN. In our previous publication, we reported that the photocatalytic degradation of 4-CP by GCN/Ag 2 CrO 4 was better than GCN [21], while in this study (Figure 2a,b) we find a better performance was observed for GCN than Ag 2 CrO 4 in the photocatalytic degradation of 2,4-D and Mecoprop-P. This indicates that the photocatalytic degradation performance of Ag 2 CrO 4 is contaminant-specific. This can be attributed to the bond energies existing in the contaminants such as C-C, C=C, O-H, C=O, and C-Cl. Bond energy or bond dissociation energy is the measure of bond strength, and it is an indication of the easiness to break a chemical bond. The bond energies of bonds existing in 4-CP, 2,4-D, and Mecoprop-P are shown in Table 2. There are fewer bonds in 4-CP than 2,4-D and Mecoprop-P that need to be broken by ROS generated from Ag 2 CrO 4 . In 4-CP, apart from the aromatic ring, there are C-Cl, C-O, and O-H corresponding to 327 KJ/mol, 358 KJ/mol, and 459 KJ/mol, while 2,4-D and Mecoprop-P have all the bonds listed in Table 2 in addition to the aromatic ring. This means with 2,4-D and Mecoprop-P having more bonds and higher bond energies, they will be more difficult to break by the ROS generated from Ag 2 CrO 4 and hence the observed low photocatalytic performance compared to 4-CP. The low surface area of Ag 2 CrO 4 , in addition to the bond energies of the contaminants, also would have contributed to the low performance. With a specific surface area of 1.32 m 2 /g [21], it means that less absorption of 2,4-D and Mecoprop-P would take place for sufficient photocatalytic activity compared to single contaminant absorption of 4-CP. Therefore, the combination of the low surface area of Ag 2 CrO 4 for the absorption of the two contaminants and the several bonds and higher bond energies of 2,4-D and Mecoprop-P contributed to the observed low performance of Ag 2 CrO 4 .
In addition, in the photocatalytic degradation of Mecoprop-P, the aromatic ring is hydroxylated to the main by-product, 4-chloro-o-cresol, followed by other by-products such as 2-methyl hydroquinone, and 2-methyl-p-benzoquinone [37] as proposed in Equations (6)- (8).

Photocatalytic Degradation of BSA Protein and SARS-CoV-2 Spike Protein
The interaction between the synthesized photocatalysts and BSA in the absence of light (dark experiment) was investigated and the results are shown in Figure 3a. It was observed that the concentration of BSA did not change when GCN was present in the solution, indicating that the GCN had a negligible impact in the dark experiment. However, it was observed that the concentration of BSA decreased significantly by 93.1% in 30 min in the presence of GCN/Ag 2 CrO 4 . The photocatalytic degradation results of BSA are presented in Figure 3b. It shows that royal blue LED irradiation alone and GCN/royal blue LED irradiation could not degrade BSA. The royal blue LED with peak wavelength of 460 nm and low energy (2.7 eV) cannot alone degrade BSA with absorption peak of 280 nm and high energy (4.4 eV) indicating that BSA requires high energy radiation greater than 4.4 eV for degradation to take place. The GCN/royal blue LED could not degrade BSA because of the high electron-hole pair recombination rate of GCN owing to its medium band gap (2.7 eV). However, more than 99.9% of BSA was degraded in 20 min in the presence of GCN/Ag 2 CrO 4 under royal blue LED irradiation. The first-order rate constant (k) values of GCN and GCN/Ag 2 CrO 4 were calculated, and they are 0.002 × 10 −1 min −1 and 3.64 × 10 −1 min −1 respectively.  In a recently published work, the photocatalytic degradation mechanism of protein using silver nanoparticles (AgNPs) was reported by investigating the effects of the following 3-step phenomenon; release of silver ions, generation of reactive oxidative species (ROS), and the light-induced protein oxidation through the bind-and-damage model [62]. It was postulated and concluded by asserting an alternate mechanism, which is that lightinduced protein oxidation is responsible for the degradation rather than the popular notions that the mechanisms of Ag + release from AgNPs and the generation of ROS are the driving forces for protein degradation and bacterial death. For the bind-and-damage model of the light-induced protein oxidation to take place, the AgNPs released the absorbed visible light energy in the form of emission. This emission of light is then quenched by a protein which indicates that the absorbed light energy was transferred to the protein, causing structural damage to the proteins.
The assertions were applied to this work for verification by carrying out UV-vis absorption spectra measurements of BSA for GCN and GCN/Ag2CrO4 with and without royal blue LED irradiation to further understand the degradation profile and the change in concentration with time. UV-vis, as an analytical method, can be used in absorption spectra measurement in understanding structural changes to protein and the interaction between protein and nanoparticles [49,50,63,64]. The observation was that the phenomena: release of Ag + from the Ag2CrO4, generation of ROS through photocatalytic reaction, and the bind-and-damage model of the light-induced protein oxidation, have varying degrees of contribution to the degradation of protein in the presence of GCN/Ag2CrO4 with and without blue LED irradiation. The roles these phenomena play in either protein degradation or bacterial death will depend on the type of photocatalyst, light intensity, radiation spectrum, and energy of the light source. In this work, two ions (Ag + and CrO4 2− ) were released against Ag + of AgNPs since GCN/Ag2CrO4 composite was used, indicating the possibility of the impact of CrO4 2− in the degradation mechanism as an electron scavenger during photocatalytic activity. The UV-vis absorption spectrum in Figure 4 shows the effect of GCN and GCN/Ag2CrO4 on BSA with and without royal blue LED irradiation. At time zero (Figure 4a-d), BSA shows the typical absorption peaks at a lower wavelength of 200 nm and a higher wavelength of 280 nm. The absorption peak at 200 nm is attributed to the BSA's polypeptide backbone and the absorption peak at 280 nm is due to its aromatic amino acids (tryptophan, tyrosine, and phenylalanine). In Figure 4a,c, the GCN without and with royal blue LED irradiation revealed similar absorption spectra. In Figure 4a, without light (dark), the BSA peak did not change because the conditions required for protein damage which are light-induced protein oxidation and ROS generation are not present since photocatalytic activity did not take place. It also indicates no interaction between the BSA and GCN, hence no structural change to the protein. In Figure 4c, under royal blue LED irradiation, the bind-and-damage model of the light-induced In a recently published work, the photocatalytic degradation mechanism of protein using silver nanoparticles (AgNPs) was reported by investigating the effects of the following 3-step phenomenon; release of silver ions, generation of reactive oxidative species (ROS), and the light-induced protein oxidation through the bind-and-damage model [62]. It was postulated and concluded by asserting an alternate mechanism, which is that light-induced protein oxidation is responsible for the degradation rather than the popular notions that the mechanisms of Ag + release from AgNPs and the generation of ROS are the driving forces for protein degradation and bacterial death. For the bind-and-damage model of the light-induced protein oxidation to take place, the AgNPs released the absorbed visible light energy in the form of emission. This emission of light is then quenched by a protein which indicates that the absorbed light energy was transferred to the protein, causing structural damage to the proteins.
The assertions were applied to this work for verification by carrying out UV-vis absorption spectra measurements of BSA for GCN and GCN/Ag 2 CrO 4 with and without royal blue LED irradiation to further understand the degradation profile and the change in concentration with time. UV-vis, as an analytical method, can be used in absorption spectra measurement in understanding structural changes to protein and the interaction between protein and nanoparticles [49,50,63,64]. The observation was that the phenomena: release of Ag + from the Ag 2 CrO 4 , generation of ROS through photocatalytic reaction, and the bind-and-damage model of the light-induced protein oxidation, have varying degrees of contribution to the degradation of protein in the presence of GCN/Ag 2 CrO 4 with and without blue LED irradiation. The roles these phenomena play in either protein degradation or bacterial death will depend on the type of photocatalyst, light intensity, radiation spectrum, and energy of the light source. In this work, two ions (Ag + and CrO 4 2− ) were released against Ag + of AgNPs since GCN/Ag 2 CrO 4 composite was used, indicating the possibility of the impact of CrO 4 2− in the degradation mechanism as an electron scavenger during photocatalytic activity. The UV-vis absorption spectrum in Figure 4 shows the effect of GCN and GCN/Ag 2 CrO 4 on BSA with and without royal blue LED irradiation. At time zero (Figure 4a-d), BSA shows the typical absorption peaks at a lower wavelength of 200 nm and a higher wavelength of 280 nm. The absorption peak at 200 nm is attributed to the BSA's polypeptide backbone and the absorption peak at 280 nm is due to its aromatic amino acids (tryptophan, tyrosine, and phenylalanine). In Figure 4a,c, the GCN without and with royal blue LED irradiation revealed similar absorption spectra. In Figure 4a, without light (dark), the BSA peak did not change because the conditions required for protein damage which are light-induced protein oxidation and ROS generation are not present since photocatalytic activity did not take place. It also indicates no interaction between the BSA and GCN, hence no structural change to the protein. In Figure 4c, under royal blue LED irradiation, the bind-and-damage model of the light-induced protein oxidation and the generated ROS did not have an impact on the degradation of the protein. This suggests that the emitted light from the absorbed light energy by the GCN was neither sufficient nor transferred to the protein for degradation, and the generated ROS was not sufficient to cause degradation because of the GCN's poor charge transfer and fast electron-hole recombination rate characteristics. In the presence of GCN/Ag 2 CrO 4 (Figure 4b,d), an absorption peak at 374 nm was observed. This peak is attributed to the presence of silver chromate in the BSA slurry. Silver compounds are known to have absorption peaks between 320 nm and 410 nm [51,52,65]. In Figure 4b,d, the GCN/Ag 2 CrO 4 without and with royal blue LED irradiation show similar spectral patterns. In Figure 4b, without light (dark), Ag + and CrO 4 2− were released by Ag 2 CrO 4 in the GCN/Ag 2 CrO 4 nanocomposite. The interactions of the Ag + with BSA resulted in higher BSA absorption peaks compared to GCN without light (dark). In Figure 4d, under royal blue LED irradiation the bind-anddamage model light-induced protein oxidation and the ROS generation did not significantly contribute to the protein degradation since the peaks without light and with royal blue LED are similar. This is evident in the change in BSA concentration when exposed to GCN/Ag 2 CrO 4 with and without royal blue LED irradiation (Figure 3a,b), where the degradation is similar at 90% and 99% in 15 min respectively. Therefore, we can conclude the following: (1) the Ag + released from GCN/Ag 2 CrO 4 was largely responsible for BSA protein degradation with and without royal blue LED irradiation, and (2) the bind-anddamage model of light-induced protein oxidation and the generation of ROS did not significantly contribute to photocatalytic degradation of BSA protein, indicating that the light irradiation had less impact in the photocatalytic process. The less impact of light irradiation is expressed in the proximities of the degradation kinetics of GCN/Ag 2 CrO 4 with royal blue irradiation (3.64 × 10 −1 min −1 ) which is less than two times without light, that is dark (1.86 × 10 −1 min −1 ). The reason for the dominance of these silver ions in the degradation of BSA protein could be attributed to their binding interactions with BSA. Previous studies show that silver ions and silver nanoparticles interact with protein functional groups, such as carboxylic groups (COOH), thiol groups (SH), and amino groups (NH) of protein, to form bonds resulting in protein inactivation [51,[66][67][68][69]. Zaher et al. investigated the interaction between Ag + and AgNPs with BSA protein and reported that Ag + has stronger binding and quenching efficiency towards BSA than AgNPs which is evidenced in a higher binding constant of Ag + [70]. BSA is a polymer of about 583 amino acids joined together by peptide linkage between two tryptophan residues. Each amino acid has two pH-sensitive functional groups, COOH and NH. The proposed binding mechanism was due to the electrostatic attraction between the ionized COO − and the positive charge of Ag + at physiological pH (pH = 7.4). This was confirmed in the reported UV-vis spectra showing the peak intensity increasing with rising Ag + concentration. This correlates with our UV-vis spectra results for GCN/Ag 2 CrO 4 with and without royal blue LED irradiation where the BSA peak intensity increases indicating a rise in Ag + concentration with time in the reacting medium and therefore the increasing interaction between the COO − and Ag + electrostatically. This interaction leads to denaturing, unfolding, aggregation, and the eventual inactivation of the protein. Jaiswar et al. investigated the binding interaction between protein and chromate ions and reported structural changes to protein, indicating successful interactions [71].
In Figure 5, SARS-CoV-2 (Coronavirus) spike protein result is presented. It shows that GCN/Ag 2 CrO 4 also can degrade the spike protein under royal blue LED. A photocatalytic degradation efficiency of 77.5% was achieved in 30 min. The lower degradation efficiency observed for SARS-CoV-2 spike protein (SP) can be attributed to its heavier molecular weight and structure. SARS-CoV-2 SP is 135 kDa and 1213 amino residues which is twice the size of BSA protein which is 66 kDa and 583 amino acid residues.

Photocatalytic Disinfection of Wastewater Secondary Effluent
Results on the disinfection of secondary effluent from a municipal wastewater treatment plant by GCN/Ag2CrO4 are presented in Figures 6 and 7. The log reduction of cellular Adenosine Triphosphate (cATP) is presented in Figure 6, while the log reduction of

Photocatalytic Disinfection of Wastewater Secondary Effluent
Results on the disinfection of secondary effluent from a municipal wastewater treatment plant by GCN/Ag2CrO4 are presented in Figures 6 and 7. The log reduction of cellular Adenosine Triphosphate (cATP) is presented in Figure 6, while the log reduction of Photocatalytic degradation of SARS-CoV-2 (Coronavirus) spike protein using GCN/Ag 2 CrO 4 . Under royal blue LED irradiation.

Photocatalytic Disinfection of Wastewater Secondary Effluent
Results on the disinfection of secondary effluent from a municipal wastewater treatment plant by GCN/Ag 2 CrO 4 are presented in Figures 6 and 7. The log reduction of cellular Adenosine Triphosphate (cATP) is presented in Figure 6, while the log reduction of total coliforms and E. coli is presented in Figure 7. As shown in Figure 6, around 0.2 log reduction of cATP was observed after 60 min of royal blue LED irradiation or after 60 min of dark (control experiment). The presence of GCN/Ag 2 CrO 4 in the dark or royal blue LED irradiation both increased the log reduction of cATP in wastewater. Moreover, 1.2 log reduction was observed for cATP when wastewater was mixed with GCN/Ag 2 CrO 4 in the dark for 60 min. An even higher log reduction (>1.7) was observed in the presence of GCN/Ag 2 CrO 4 under royal blue LED irradiation for 60 min.
Catalysts 2022, 12, x FOR PEER REVIEW 10 total coliforms and E. coli is presented in Figure 7. As shown in Figure 6, around 0 reduction of cATP was observed after 60 min of royal blue LED irradiation or after 6 of dark (control experiment). The presence of GCN/Ag2CrO4 in the dark or royal blue irradiation both increased the log reduction of cATP in wastewater. Moreover, 1.2 l duction was observed for cATP when wastewater was mixed with GCN/Ag2CrO4 dark for 60 min. An even higher log reduction (>1.7) was observed in the presen GCN/Ag2CrO4 under royal blue LED irradiation for 60 min. In the control experiments of royal blue LED and GCN (dark experiment), the coliforms and E. coli did not decrease during the experimental duration of 60 min indicates that the royal blue LED irradiation and GCN (dark experiment) did not im the total coliforms and E. coli counts in the wastewater. The dark control experim GCN/Ag2CrO4 and the use of GCN under royal blue LED both led to lower log redu (<1) in 60 min for total coliforms and E. coli. However, the use of GCN/Ag2CrO4 u royal blue LED irradiation led to higher log reduction (>1) in 60 min with 2.43 log r tion of total coliforms and 1.48 log reduction of E. coli. This result indicates that the fection rate of E. coli was slower than that of the total coliforms.
The interaction of silver compounds with the cell membrane of the bacteria is o the important mechanisms of silver compound toxicity [54]. This can occur both dark and visible light irradiation. Over one log reduction was observed for ATP coliforms, and E. coli in 60 min during the photocatalytic degradation process. This cates GCN/Ag2CrO4 can potentially photocatalytically inactivate, under royal blue irradiation, over 98% (1.7 log) of the microbial activity (ATP) in 60 min which inc inactivation of over 99.6% (2.43 log) of total coliforms.
The bind-and-damage model of protein oxidation, release of ions from the phot lyst, and the ROS generation processes of protein degradation described in Section 3 be adopted for this disinfection process since it was used for protein degradation and terial disinfection by Shi et al. [62]. In Figure 6, the GCN/Ag2CrO4 with and without blue LED irradiation showed a significant impact on the reduction of cATP of mi ganisms. Without light (GCN/Ag2CrO4 + Dark), the release of Ag + and CrO4 2-contri to the reduction of cATP by 1.2 log in 60 min. However, with irradiation (GCN/Ag + Royal blue LED), the ROS generation in addition to Ag + and CrO4 2-released res in >1.7 log reduction of cATP in 60 min. With the marginal difference of >0.5 log redu of cATP, it means the release of the two ions contributed far more than ROS generat In the control experiments of royal blue LED and GCN (dark experiment), the total coliforms and E. coli did not decrease during the experimental duration of 60 min. This indicates that the royal blue LED irradiation and GCN (dark experiment) did not impact the total coliforms and E. coli counts in the wastewater. The dark control experiment of GCN/Ag 2 CrO 4 and the use of GCN under royal blue LED both led to lower log reduction (<1) in 60 min for total coliforms and E. coli. However, the use of GCN/Ag 2 CrO 4 under royal blue LED irradiation led to higher log reduction (>1) in 60 min with 2.43 log reduction of total coliforms and 1.48 log reduction of E. coli. This result indicates that the disinfection rate of E. coli was slower than that of the total coliforms.
The interaction of silver compounds with the cell membrane of the bacteria is one of the important mechanisms of silver compound toxicity [54]. This can occur both in the dark and visible light irradiation. Over one log reduction was observed for ATP, total coliforms, and E. coli in 60 min during the photocatalytic degradation process. This indicates GCN/Ag 2 CrO 4 can potentially photocatalytically inactivate, under royal blue LED irradiation, over 98% (1.7 log) of the microbial activity (ATP) in 60 min which includes inactivation of over 99.6% (2.43 log) of total coliforms.
The bind-and-damage model of protein oxidation, release of ions from the photocatalyst, and the ROS generation processes of protein degradation described in Section 3.2 can be adopted for this disinfection process since it was used for protein degradation and bacterial disinfection by Shi et al. [62]. In Figure 6, the GCN/Ag 2 CrO 4 with and without royal blue LED irradiation showed a significant impact on the reduction of cATP of microorganisms. Without light (GCN/Ag 2 CrO 4 + Dark), the release of Ag + and CrO 4 2− contributed to the reduction of cATP by 1.2 log in 60 min. However, with irradiation (GCN/Ag 2 CrO 4 + Royal blue LED), the ROS generation in addition to Ag + and CrO 4 2− released resulted in >1.7 log reduction of cATP in 60 min. With the marginal difference of >0.5 log reduction of cATP, it means the release of the two ions contributed far more than ROS generation in the overall reduction of cATP. In Figure 7a, the GCN/Ag 2 CrO 4 with and without royal blue LED irradiation showed a different response to the disinfection of total coliforms. Without light (GCN/Ag 2 CrO 4 + Dark), the release of Ag + and CrO 4 2− resulted in a 0.3 log reduction of total coliforms in 60 min while the irradiation (GCN/Ag 2 CrO 4 + Royal blue LED), the ROS generation and the light-induced protein oxidation in addition to the released two ions resulted in 2.4 log reduction of total coliforms in 60 min. The log reduction difference >2 log is largely due to the contributions of the generated ROS and the light-induced protein oxidation. In Figure 7b, the GCN/Ag 2 CrO 4 , with and without royal blue LED irradiation showed a different response to E. coli disinfection. Without light (GCN/Ag 2 CrO 4 + dark), the Ag + and CrO 4 2− released resulted in a 0.1 log reduction of E. coli in 60 min while with irradiation (GCN/Ag 2 CrO 4 + Royal blue LED), the generated ROS and light-induced protein oxidation in addition to the released ions resulted in 1.5 log reduction of E. coli in 60 min. The log reduction difference >1 log is largely due to the contributions of the ROS generated and the light-induced protein oxidation.
Catalysts 2022, 12, x FOR PEER REVIEW 11 of 20 the overall reduction of cATP. In Figure 7a, the GCN/Ag2CrO4 with and without royal blue LED irradiation showed a different response to the disinfection of total coliforms. Without light (GCN/Ag2CrO4 + Dark), the release of Ag + and CrO4 2− resulted in a 0.3 log reduction of total coliforms in 60 min while the irradiation (GCN/Ag2CrO4 + Royal blue LED), the ROS generation and the light-induced protein oxidation in addition to the released two ions resulted in 2.4 log reduction of total coliforms in 60 min. The log reduction difference >2 log is largely due to the contributions of the generated ROS and the lightinduced protein oxidation. In Figure 7b, the GCN/Ag2CrO4, with and without royal blue LED irradiation showed a different response to E. coli disinfection. Without light (GCN/Ag2CrO4 + dark), the Ag + and CrO4 2-released resulted in a 0.1 log reduction of E. coli in 60 min while with irradiation (GCN/Ag2CrO4 + Royal blue LED), the generated ROS and light-induced protein oxidation in addition to the released ions resulted in 1.5 log reduction of E. coli in 60 min. The log reduction difference >1 log is largely due to the contributions of the ROS generated and the light-induced protein oxidation.  Zhang et al. investigated the impact of light irradiation on the reduction of Ag + to AgNPs by bacteria and reported that visible lights (monochromatic light at wavelengths 415 nm and 600 nm) accelerated the reduction of Ag + to AgNPs by bacteria and almost no AgNPs were formed in the dark [72]. Furthermore, the visible light irradiation reversibly can excite the AgNPs for their surface plasmon resonance (SPR) and accelerate the electrons from the bacteria to the adjacent Ag + . It reported that the bacteria can reduce Ag + and the process can be accelerated by light irradiation. Shi et al. focused on the bactericidal effect of AgNPs and identified a new antibacterial mechanism different from the ROSinduced photocatalyst [62]. AgNPs can penetrate the cell wall and membrane and gather in the cytosol and bind directly with the cytosol protein leading to the inducement of protein aggregation, an indication of protein degradation, caused by the bind-and-damage model of the light-induced protein oxidation. Deng et al. investigated the effects of silver ions, chromium VI, Cr(VI), and ROS on the photocatalytic disinfection mechanism of E. coli [29]. The results show that the combined effects of the release of silver ion and the generation of ROS of holes (h + ) and hydroxyl ( . OH) radicals played important roles in the photocatalytic disinfection process. The silver ions interact with the thiol (-SH) functional group causing cell damage and the generated ROS could inactivate bacterial cells by interacting with the cell wall and destabilizing the membrane causing leakage of potassium ions (K + ). The interaction of Ag + with bacterial cell membranes can also lead to lower ATP readings caused by the leakage of potassium ions [54]. The addition of hexavalent chromium to the photocatalytic system enhanced the photocatalytic disinfection kinetics. This is because Cr(VI) can behave as an electron scavenger, directly reduces electron-hole recombination, enhances the amount of h + available for disinfection, and ultimately leads to better disinfection performance. Table 4 shows the photocatalytic disinfection of microorganisms by different photocatalysts.

Preparation of GCN, Ag 2 CrO 4 , and GCN/Ag 2 CrO 4 Nanocomposite
The GCN, Ag 2 CrO 4, and GCN/Ag 2 CrO 4 were synthesized based on a procedure described in detail elsewhere [21]. The GCN was synthesized using the direct pyrolysis method by heating urea at 550 • C for 4 h in a muffle furnace. The GCN/Ag 2 CrO 4 composite was synthesized using the dark-induced/In-situ-deposition method by first sonicating one gram of GCN slurry. A known quantity of AgNO 3 and 4.83 g/L K 2 CrO 4 were added to the GCN slurry under magnetic stirring. The mixture was then magnetically stirred in the dark for 7 h and after that, it was filtered using a 0.45 µm filter and washed about five times with water and alcohol. The residue was left to dry at room temperature and thereafter labeled as GCN/Ag 2 CrO 4 . The characterizations of these catalysts have been reported elsewhere [21].

Photocatalytic Degradation of a Commercial Herbicide
Killex R is a commercially-available herbicide that contains 95 g/L of 2,4-dichlorophen oxyacetic acid (2,4-D), 52.52 g/L of methyl phenoxy propionic acid (Mecoprop-P or MCPP), and 9 g/L of Dicamba. A quantity of 0.8 mL of Killex R was dissolved in 79.2 mL of distilled water (DI) which gave an 80 mL solution, resulting in the solution containing 12.1 mg/L of 2,4-D, 6.7 mg/L of Mecoprop-P, and 1.2 mg/L of Dicamba. In this experiment, 80 mg of the photocatalyst was dispersed in an 80 mL solution containing Killex R in a 100 mL quartz beaker. The intensity of the royal blue LED (peak wavelength = 460 nm) entering the 80 mL solution was estimated to be 11.80 × 10 17 photons/s using chemical ferrioxalate actinometry [43,76,77]. The same photoreactor was used for all experiments mentioned in this study. Before LED irradiation, the water/catalysts mixture was stirred magnetically in the dark for 30 min to attain adsorption-desorption equilibrium. Quantities of 5 mL of the samples were collected at different irradiation intervals and filtered using a 0.45 µm syringe filter (PTFE, Chromatographic Specialties Inc., Brockville, ON, Canada) to remove the photocatalysts. Dark experiments with catalysts and light experiments without catalysts were also conducted as controls. HPLC was used to evaluate the concentration change of 2,4-D and Mecoprop-P.

Photocatalytic Degradation of BSA Protein and SARS-CoV-2 Spike Protein
In each experiment, 80 mg of the photocatalyst was dispersed in 80 mL of 100 mg/L BSA solution. During royal blue LED irradiation, 6 mL of sample was collected at different irradiation intervals and filtered using a 0.45 µm syringe filter to remove the photocatalysts. The performance of GCN/Ag 2 CrO 4 was also investigated on SARS-CoV-2 spike protein (SP) under royal blue LED. In the experiment, 40 mg of the GCN/Ag 2 CrO 4 was dispersed in a 40 mL of 10 mg/L SARS-CoV-2 SP, and 3 mL of the sample was collected at a different irradiation interval and filtered using a 0.45 µm syringe filter to remove the photocatalysts. Bradford assay procedure was used to determine the concentration change for the BSA protein and SARS-CoV-2 SP [78]. UV-Vis analysis was conducted for visible light and dark experiments to study the binding interaction between BSA and GCN/Ag 2 CrO 4 .

Photocatalytic Disinfection of Wastewater Secondary Effluent
Two sets of disinfection experiments were conducted. The first one was focused on the change of cATP level on the collected wastewater during the disinfection experiment, while the second one was aimed at quantifying the log reduction of total coliforms and E. coli by the catalyst under royal blue LED irradiation. For the cATP experiment, 80 mg of the photocatalyst was dispersed in 80 mL of wastewater secondary effluent and then irradiated with royal blue LED under continuous magnetic stirring. A total of 2.5 mL of the sample was taken at a different time interval and its cATP level was quantified using the LuminUltra test procedure for assay analysis [79]. Control experiments were carried out in the dark with the photocatalyst and under the light without the photocatalyst. Before the start of the disinfection experiment, the initial concentrations of total coliforms and E. coli were calculated using the tray count method [80]. A stock solution of 1 L secondary treated wastewater effluent containing 50 mg of Luria broth nutrient was first prepared in a 1 L jar and then incubated at 35 • C ± 0.5 • C for 24 h. Then 1 mL of the 80 mL collected from the stock solution was serially diluted 40 times as follows: in the first mixture, 1 mL was added to 3 mL of deionized (DI) water. After mixing, 1 mL from the first mixture was added to 9 mL of DI water to give 40 times serial dilution, this gives the second mixture. Finally, using the tray count method, 1 mL of the second mixture was added to the mixture of 100 mL DI water and colilert reagent, giving the final dilution factor of 4000. The final mixed sample was poured in a quantitray, sealed with an IDEXX sealer, and incubated at 35 • C ± 0.5 • C for 24 h. The tray, which has 49 large wells and 48 small wells, was used to estimate the most probable number (MPN) of coliforms and E. coli in a 100 mL wastewater sample. The actual MPN/100 mL of total coliforms or E. coli present in the sample was calculated by multiplying the MPN by the dilution factor of 4000. The average initial concentrations of total coliforms and E. coli were calculated to be 5.2 × 10 6 MPN/100 mL and 0.25 × 10 6 MPN/100 mL respectively. Disinfection experiments were conducted and MPNs of total coliforms and E. coli were estimated and concentrations at different time intervals were analyzed and calculated using the tray count method. Control experiments were also performed in the dark with photocatalysts and under the light without photocatalysts.

Herbicide Analysis
The concentrations of 2,4-D and Mecoprop-P were determined using high-performance liquid chromatography (HPLC; LC-2040C 3D Shimadzu Corporation, Kyoto, Japan) with UV absorbance detection at λ = 280 nm. A kinetex 2.6 µm PFP column by Phenomenex (Torrance, CA, USA) was used to separate the target compounds and other interferences. The mobile phases were 0.1% phosphoric acid in HPLC grade water and 0.1% phosphoric acid in acetonitrile at a 50:50 mixture with a flow rate of 1 mL/min. The measurements were conducted in duplicate, and the detection limit was around 0.1 mg/L.

Protein Analysis
The concentration of BSA protein was determined using the Bradford protein assay procedure [78]. This procedure is based on the binding of Coomassie brilliant blue dye to protein. Briefly, the diluted dye was prepared by mixing concentrated Coomasie dye (80 mL) and deionized water (DI) of 240 mL and then filtered with 5 µm using vacuum filtration to remove dye particles. From the BSA stock solution (2000 mg/L), five standard dilute solutions were prepared (100 mg/L, 50 mg/L, 25 mg/L, 12.5 mg/L and 6.25 mg/L). Then, 0.8 mL of each standard and photocatalytic degraded BSA samples were collected into the vial using a pipette and 3.2 mL of diluted dye was added. The mixture was put on a rotary shaker and left to incubate for at least 5 min but not more than 60 min at room temperature. This incubation period allows for colour change of the Coomasie dye from red to blue. This blue colour is an indication of the binding of the dye to the protein, that is detected at 595 nm. The spectrophotometer (UV-2600 Shimadzu Corp., Kyoto, Japan) was set at 595 nm to take the absorbance readings of BSA standards and the degraded samples using a disposable cuvette. The concentration of the degraded samples was calculated based on the known concentrations of the BSA standard.
This Bradford protein assay procedure was also used to determine the concentration of the SARS-CoV-2 SP. Five standard solutions of BSA of known concentrations (10 mg/L, 5 mg/L, 2.5 mg/L, and 0.625 mg/L) were prepared. Using the microassay plate, 800 µL of each standard and degraded SARS-CoV-2 SP sample was put in a vial and 200 µL of dye concentrate was added to have the protein-dye mixture. The same incubation period as described earlier was used for the spike protein and after that, the absorbance of the SP-dye complex was read at 595 nm using a spectrophotometer (UV-2600 Shimadzu Corp., Kyoto, Japan).
The UV-vis as an analytical technique was used to study the BSA and photocatalyst interaction and the structural changes to BSA. The UV-vis absorption spectra measurements were carried out for the dark as well as royal blue irradiation samples.

ATP Analysis
The concentration of the microorganisms' cATP was determined using the LuminUltra test kit and assay procedure [80]. A total of 5 mL of the water sample was passed through a 0.45 µm syringe filter. The syringe filter containing microorganisms was re-attached to a clean syringe with the plunger removed, and then 1 mL of UltraLyse 7 was pipette into the syringe. The filtration was done slowly, and the sample was collected in a 9 mL UltraLute (dilution) tube. The purpose of the filtration using UltraLyse 7 is to be able to dissolve and remove the bound ATP (ATP in living microorganisms after disinfection) for analysis. A quantity of 100 µL of UltraLute (dilution) solution was pipette into a clean test tube and then 100 µL Luminase was added. The mixture was shaken about five times, and then the reading was taken within 10 s using a luminometer. The cellular ATP is calculated using Equation (9) and the log reduction of the normalized concentration is evaluated using Equation (10): cATP pg ATP mL = RLU cATP RLU ATP1 × 10, 000 pg ATP V sample (mL) (9) Log reduction = − log cATP t cATP o (10) where RLU is the unit of measurement as it is the relative light unit, pg is picogram, V sample is the volume of the sample collected during the photocatalytic experiment, ATP is the calibrated ATP RLU value for the wastewater before the photocatalytic experiment, and cATP o and cATP t are the cellular ATP of the microorganisms at time, t = 0 and t = t min. Control experiments were conducted in the dark to determine the adsorption performance of the photocatalysts in disinfecting microorganisms and the corresponding estimation of ATP levels.

Total Coliform/E. coli Analysis
One mL sample was collected at different irradiation intervals and serially diluted using deionized water. This was aimed to (1) reduce the amount of catalysts and other water matrices present in the final tray so as to minimize their interferences on total coliform and E. coli measurement; (2) to ensure the concentration of total coliform and E. coli fall within the measurement range of the Colilert tray count procedure. The total coliforms and E. coli concentrations were determined using the tray count method [80] as described in Section 3.3.3. The total coliform and E. coli disinfection efficiency were expressed in log form as shown in Equation (11): where C o and C t are the concentrations of total coliforms or E. coli before and after time t min, expressed as MPN/100 mL.

Conclusions
A visible-light active photocatalyst, GCN/Ag 2 CrO 4 nanocomposite was synthesized, and its performance was investigated in the degradation of organic pollutants (2,4-D and Mecoprop-P) in Killex R , BSA protein, and SARS-CoV-2 spike protein) and the disinfection of WWTP secondary effluent, using a narrowband low-energy visible light source (royal blue LED at 460 nm). The results from this study highlight the following:

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Similar degradation rates and percentage removal visible light irradiation were observed for 2,4-D and Mecoprop-P.

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The degradation performance of a photocatalyst is dependent on the available surface area for absorption for photocatalytic activity and the bond energies of the chemical bonds that exist in the contaminant.

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Released Ag + and CrO 4 2− from the GCN/Ag 2 CrO 4 nanocomposite were largely responsible for the BSA degradation and cATP reduction. • Generated ROS were largely responsible for the disinfection of total coliforms and E. coli.

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The lower percentage degradation (70%) of SARS-CoV-2 spike protein can be attributed to its large molecular weight and structure compared to BSA protein.

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The pathways for degradation of proteins and the disinfection of microorganisms depend on any of the following: (1) binding affinity of the released metal ions from GCN/Ag 2 CrO 4 with the functional groups of proteins and ATP, (2) amount of ROS generated, and (3) the quantum of emission light from the photocatalyst that is absorbed and quenched by the proteins because of the light-induced protein oxidation. • Significant disinfection results observed in the reduction of microorganisms' cATP, total coliforms, and E. coli with GCN/Ag 2 CrO 4 are also due to the reduction of the released Ag + to AgNPs by bacteria which was accelerated by light irradiation.

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Over one log reduction of microorganisms' cATP, total coliforms, and E. coli in wastewater secondary effluent was achieved in 60 min.

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The combination of GCN/Ag 2 CrO 4 and the narrowband low-energy light (royal blue LED at peak wavelength = 460 nm) holds promise in that it can be used in the wastewater treatment plant to degrade multiple recalcitrant organic pollutants and inactivate microorganisms.

Data Availability Statement:
The data presented in this study are available on request from the corresponding author.