Enzymatic Synthesis, Structural Analysis, and Evaluation of Antibacterial Activity and α-Glucosidase Inhibition of Hesperidin Glycosides

This study was designed to investigate the structure of synthesized hesperidin glycosides (HGs) and evaluate their antibacterial and α-glucosidase inhibitory activities. The preliminary structure of HGs was confirmed by glucoamylase treatment and analyzed on thin layer chromatography (TLC). The LC-MS/MS profiles of HGs showed the important fragments at m/z ratios of 345.21 (added glucose to glucose of rutinose in HG1) and 687.28 (added maltose to glucose of rutinose in HG2), confirming that the structures of HG1 and HG2 were α-glucosyl hesperidin and α-maltosyl hesperidin, respectively. In addition, 1H and 13C-NMR of hesperidin derivatives were performed to identify their α-1,4-glycosidic bonds. The MIC and MBC studies showed that transglycosylated HG1 and HG2 had better antibacterial and bactericidal activities than hesperidin and diosmin, and were more active against Staphylococcus aureus than Escherichia coli. Hesperidin, HG1, HG2, and diosmin inhibited α-glucosidase with IC50 values of 2.75 ± 1.57, 2.48 ± 1.61, 2.36 ± 1.48, and 2.99 ± 1.23 mg/mL, respectively. The inhibition kinetics of HG2 shown by a Lineweaver–Burk plot confirmed HG2 was an α-glucosidase competitive inhibitor with an inhibitor constant, Ki, of 2.20 ± 0.10 mM. Thus, HGs have the potential to be developed into antibacterial drugs and treatments for treating α-glucosidase-associated type 2 diabetes.


Introduction
Flavonoids are a large group of polyphenolic compounds that are found in fruit, green leaves and vegetables. Flavonoids are characterized by the presence of 15 carbon atoms in a basic skeleton C6-C3-C6 phenyl-benzopyran structure. They can be found in two different forms: the free aglycone forms and combined sugar molecules as glycosides [1]. Flavonoids consist of six main structural classes: flavones, flavonols, flavanones, flavanonols, flavanols and anthocyanin [2]. Hesperidin, one of the most common flavonoids [3], is bitter and insoluble in water but can be sweetened and made water soluble by transglycosylation with saccharides [4][5][6]. Our previous work on the transglycosylation reaction of p19bBC recombinant cyclodextrin glycosyltransferase (CGTase, E.C. 2.4.1.19) found that hesperidin is the best acceptor in the flavonoid group [5]. The transglycosylation of hesperidin using the p19bBC CGTase is able to produce large quantities of HGs (HG1-3) [5,6], but their glycoside structures are yet to be identified.
Hesperidin is widely used in clinical applications as an antioxidant [6,7], anti-inflammatory [8,9], anti-cancer [10][11][12], antibacterial [13,14] and antidiabetic [15]. Several research groups have recently investigated the possible antibacterial activity of hesperidin [13,16]. In plants, hesperidin protects against bacterial, fungal and viral pathogens. Its antibacterial activity depends on its molecular structure, hydrophobicity, solubility, presence or absence of a sugar unit, and the type of sugar in the chemical backbone [17]. By agar diffusion method, das Neves et al. [18] demonstrated that the ethanolic extract of the grapefruit seed and pulp contained hesperidin that inhibited only Gram-positive bacteria; however, this extract was effective against both Gram-positive and Gram-negative bacteria using the broth dilution susceptibility test [19]. Yi et al. [14] evaluated the antibacterial effects of a flavonoid extract of Pericarpium Citri Reticulatae (FEPCR) and its major constituents, including hesperidin, against Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis, Salmonella typhimurium and Enterobacter cloacae using the agar dilution method. This study revealed that hesperidin and FEPCR had a wide range of antibacterial activity that was greater against the Gram-positive than the Gram-negative bacteria. The lowest and highest minimal inhibitory concentrations (MICs) were observed for S. aureus (MIC: 100 μg/mL) and E. cloacae (MIC: 1600 μg/mL), respectively. Given the growing challenge of antibacterial resistance, the search for new drugs should include natural compounds such as medicinal plants.
Hesperidin also inhibits α-glucosidase and decreases the concentration of blood glucose, a property that could be used to treat diabetes mellitus (DM). DM, a major global health challenge, is characterized by reduced insulin secretion and insulin receptor sensitivity resulting in chronic hyperglycaemia [15] and leads to the development of microvascular and macrovascular complications and increased morbidity and mortality [20]. Ahmed et al. [15] reported that hesperidin and naringin increased serum insulin concentrations and reduced serum glucose, blood glycosylated hemoglobin, and lipids in type 2 diabetic rats, consistent with the findings of Od-ek et al. [21], who showed that hesperidin partially inhibited α-glucosidase activity in vitro.
Our previous study on the small-scale biocatalytic production of HGs was performed using recombinant CGTase, derived from p19bBC E. coli cells [6]. This enzyme catalyzes four reactions: Cyclization, coupling, disproportionation and hydrolysis [5,6]. The main catalytic product from its coupling (intermolecular transglycosylation) is functional glycosides that are useful in the food, drug and cosmetic industries. The demand for using glycoside products in those industries has increased because they have better characteristics, such as solubility, stability and bioactivity, than their parent compounds.
This present work aims to use a recombinant CGTase for the upscale synthesis of HGs. We focused on identifying hesperidin and its derivative structures, and their antibacterial and α-glucosidase inhibitory activities. This is the first report that has attempted to elucidate the structure of glycosylated HG1-2 using LC-MS/MS and NMR analyses.

Production and Purification of Hesperidin Glycoside
The reaction mixture for the synthesis of HGs from β-CD as a donor and hesperidin as an acceptor was performed following previously reported methods [5,6]. The synthesis of HGs was scaled-up to 300 mL, and the reaction proceeded under the conditions according to Section 4.2. HG products were then purified using preparative HPLC, since they were separated as three main products at Rt of 4.8 (HG3), 6.2 (HG2) and 7.7 (HG1) min, respectively ( Figure 1). These products were lyophilized for further structural analysis and bioactivity determination. The conversion yield of HGs obtained in this study was found to be 38% (1.64 mM) from a 300 mL reaction mixture.

Digestion with Amylolytic Enzyme
Preliminarily structural analysis of synthesized HGs was performed by enzyme treatment, and the final deglucosylated products were then investigated by TLC analysis.
When HG1, HG2 and HG3 were treated with Rhizopus sp. glucoamylase (EC 3.2.1.3), the hesperidin glycoside HG1, HG2 and HG3 spots disappeared while the intensities of hesperidin (the highest Rf value) and glucose spots increased ( Figure 2, Lanes 4, 6 and 8). This finding suggested that the glycosidic linkage between the glucose units of HG1, HG2 and HG3 was hydrolyzed and that these glycosidic linkages were of an α-configuration. Thus, our results are consistent with HGs synthesizing of CGTase being specific for α-1,4linkage transfer.

LC-MS/MS Analysis
After HGs were synthesized and purified, their structures were investigated. LC-MS/MS was used in combination with glucoamylase treatment and NMR to determine the possible structures of the obtained HGs by the reaction of recombinant CGTase [22,23]. The LC-MS/MS spectra of hesperidin and each purified HG (HG1 and HG2) were determined by the Triple Quadrupole analyzer. The detector and electrospray mass spectrometry (ESI-MS) were performed in full scan mode and tandem MS n fragmentations. The mass of each purified fraction from preparative HPLC was subjected to ESI-MS 1 analysis. The products at Rt of 6.2 and 7.7 min were identified as hesperidin maltoside (HG2, hesperidin + maltose) and hesperidin glucoside (HG1, hesperidin + glucose), with masses [M + H] + at m/z of 935.30 and 773.26, respectively. Standard hesperidin had a mass at m/z pf 611.09 [6]. The fragmentation patterns of secondary mass spectrometer (MS 2 ) were performed to predict the position of glucose attached to original hesperetin and hesperidin ( Figure 3). The results in Figure 3A show the major fragmentation patterns of hesperidin that had m/z of 303.09 and 327.29 are consistent with hesperetin (peak b) and rutinose sugar (peak c), respectively. The fragmentation patterns of HG1 product at Rt 7.7 min (Figure 3B) had important peaks at m/z corresponding to the mass of hesperetin (peak c) and the mass of glucose-glucose in the HG1 structure (m/z of 345.21, peak e). The HG2 product at Rt 6.2 min ( Figure 3C) showed the mass of a sugar group linked between maltose and rutinose in the structure of HG2 (m/z of 687.28, peak f). In addition, the fragmentation pattern of HG2 still included the structure of hesperetin (m/z of 303.33, peak b). These results suggest there may be a link at 4′' OH position between the added glucose molecules with the one already attached to hesperetin.

NMR Analysis
The 1 H-and 13 C-NMR spectra of hesperetin, hesperidin and HG1-2 were determined.
According to 1 H-NMR ( Figure 4B-D), the peaks with doublet signals at 5.0-5.2 ppm (J = 3.66 Hz) were observed with a different height and assigned to the anomeric protons of each glucose, which linked to hesperidin with α-1,4-glycosidic bonds. In contrast, these peaks were not found in the 1 H-NMR spectrum of hesperetin, as shown in Figure  In 13 C-NMR spectra, carbon signals for glycoside products at Rt of 6.2 and 7.7 min were assigned to hesperidin glycosides. The peaks of HG1-2 with chemical shifts at 60-61 ppm (-CH2OH), 103-197 ppm (aromatic carbon), and 99-101 ppm (α-anomeric carbon of glucose) were observed (Table 1 and Figure S1). From the result, the α-configuration of the D-glucose residue in HG1 was identified by anomeric carbon of the glucose II signal (position C-1*) ( Table 1 and Figure 5), which shifted from 92.77 to 101.25 ppm. In addition, comparing the position C-4″ of glucose I of hesperidin and HG1 indicated that the carbon signal of HG1 was deshielded from 70.27 to 79.72 ppm, corresponding to the α-1,4-glycosidic form. For the 13 C-NMR spectrum of HG2, another α-1,4-glycosidic bond was confirmed by anomeric carbon of glucose III (the position C-1**), showing a carbon signal at 100.36 ppm. Moreover, position C-4* of HG2 was shifted down from 70.32 to 79.23 ppm compared to HG1. From the combination of these results with enzymatic treatment and C D LC-MS/MS, the structures of transglycosylated hesperidin were identified as 4 G -α-D-glucopyranosyl-hesperidin (HG1) and 4 G -α-D-maltopyranosyl-hesperidin (HG2), as illustrated in Figure 5.  Table 1) and **, the carbon positions of second added glucose (C-glucose III, Table 1)

Antibacterial Activity
HG2 showed the lowest MIC values of 4 mg/mL against S. aureus and 8 mg/mL against E. coli (Table 2). In the negative control, sterile water had no inhibitory effect on any bacteria tested. Ampicillin had good antibacterial activity against both organisms.  The MIC and MBC results showed that hesperidin glycosides had a higher antibacterial activity against Gram-positive S. aureus than hesperidin and diosmin. This result was the same as that obtained for ampicillin antibiotic, which showed better activity against Gram-positive than Gram-negative bacteria. Thus, HGs are effective agents for inhibiting S. aureus growth.

In Vitro α-Glucosidase Inhibitory Activity
As shown in Figure 6, the IC50 values of hesperidin, HG1, HG2 and diosmin were 2.75 ± 1.57, 2.48 ± 1.61, 2.36 ± 1.48 and 2.99 ± 1.23 mg/mL, respectively, whereas acarbose had an IC50 value of 1.64 ± 1.04 mg/mL. The transglycosylated HG1 and HG2 exhibited a higher inhibitory effect than original hesperidin and diosmin. Thus, adding a sugar molecule to hesperidin was a good way to inhibit α-glucosidase activity. To better understand the inhibition of hesperidin, HG2, which gave the best IC50, was chosen to determine the values of kinetic inhibition. The Lineweaver-Burk plot shows that HG2 is a competitive inhibitor of α-glucosidase (Figure 7) with an inhibitory constant (Ki) of 2.20 ± 0.10 mM. For other kinetic parameters, maximum velocity (Vmax) of α-glucosidase assayed under different conditions, with and without HG2 inhibitor, showed the same values of 0.15 ± 0.08 μmole/min. In contrast, the Michaelis constant (Km) of the enzyme on sucrose substrate without HG2 inhibitor was lower (1.34 ± 0.10 mM) compared to the condition with inhibitor (2.34 ± 0.43 mM), and its turnover number (Kcat) was 1.53 × 10 3 min −1 .

Discussion
There are many reports on transglycosylation by CGTases in relation to the synthesis of new glycosides [26][27][28][29], but few on the transglycosylation of hesperidin to hesperidin glycosides [30,31]. Kometani et al. [30] reported the formation of HGs in a small scale system at alkaline pH levels. The products with the highest yield (1.23 mM) were purified by HPLC on an ODS column. The structure of the purified hesperidin monoglucoside was identified to be 4 G -α-D-glucopyranosyl hesperidin by FAB-MS, α-and β-glucosidase, and glucoamylase treatment. They also found that the transglycosylated hesperidin had higher water soluble and stabilizing properties than hesperidin. Using a small scale system, Go et al. [31] reported a conversion yield of 71.2%. We found that the conversion yield of HGs (38%) was approximately the same as that reported previously for HGs (40%) when using an alkalophilic Bacillus sp. CGTase to catalyze the transglycosylation of glycosylated hesperidin [30].
In this investigation, we performed upscale transglycosylation of hesperidin to glycosides by CGTase, with hesperidin as the glycosyl acceptor. In order to prepare larger quantities of glycosides, the reaction mixtures were scaled up and performed under optimal conditions, and each HG product was characterized by HPLC. Each glycoside product of hesperidin was completely isolated and given only one spot on TLC ( Figure 2, lanes 3, 5 and 7). In addition, this is the first report on the structural identification of HGs by glucoamylase treatment and LC-MS/MS analysis. The structure of HGs was preliminarily analyzed in terms of the number of glucose units and their configuration. As a result, HG1 and HG2 could be digested by glucoamylase, an exohydrolase enzyme that catalyzes the hydrolysis of 1,4-linked α-D-glucose residues in glucosyl polysaccharides to liberate glucose units [32]. After hydrolysis by glucoamylase, the TLC results showed that HG1, HG2, and HG3 yielded hesperidin and glucose spots, and the molar ratios of hesperidin to glucose were 1:0.8, 1:2, and 1:3, respectively ( Figure 2, lanes 4, 6 and 8). HG1 was also digested by glucoamylase because the linkage between hesperidin and the first glucosyl unit did not directly link to the hesperetin flavonoid ring. However, the first transferred glucose was attached to the C-4″ glucose position of rutinose (a disaccharide) in the hesperidin molecule and the LC-MS/MS and NMR analysis suggested that these glycosides were linked to each glucose via an α-1,4 linkage. The molecular mass of HG1-2 was estimated at 772.26 and 934.30 daltons by ESI-MS 1 analysis, which gave a molecular ion at m/z 773 and 935 ([M + H]) + . Moreover, the ESI-MS 2 spectrum of fragmentation patterns confirmed that all observed peaks were part of the studied glycoside derivatives (Figure 3). Based on all of the results and the NMR data analysis (Figures 4 and 5 and Table 1), the structures of HG1 and HG2 were identified as 4 G -α-D-glucopyranosyl-and 4 G -α-D-maltopyranosyl-hesperidin, respectively.
We also investigated the antibacterial activities of hesperidin and HGs and found that the latter had greater antibacterial activity than original hesperidin and diosmin, especially against S. aureus. The exact mechanisms driving their antibacterial activities remain unclear, but several mechanisms have been proposed such as interference with bacterial DNA synthesis, bacterial movement, cytoplasmic membrane permeability, and the inhibition of bacterial metalloenzymes [13]. Several studies have evaluated the antibacterial activities of plant flavonoid-rich extracts and pure flavonoids, including hesperidin derivatives [14,19,33]. The ethanolic extract of grapefruit seed and pulp, containing mainly naringin and hesperidin flavanones, significantly inhibited Gram-positive bacteria (agar diffusion) and Gram-positive and Gram-negative bacteria by broth dilution [19]. In contrast to our results, Mandalari et al. [33] found that the flavonoid-rich fractions of bergamot peel had activity against Gram-negative bacteria such as E. coli, Pseudomonas putida, and Salmonella enterica but not against Gram-positive bacteria such as Listeria innocua, B. subtilis, S. aureus, and Lactococcus lactis. The treatment of these fractions with pectinase 62 L [33], which converted flavonoid glycosides into their aglycones, increased their antibacterial activity. Moreover, they studied the antibacterial activities values of pure bergamot flavonoids (aglycone forms), neohesperidin, heperetin, neoeriocitrin, eriodictyol, naringin and naringenin, and all MIC values were 250-1000 μg/mL. Similar to our work, Lee et al. [34] improved the bioavailability and the water solubility of hesperidin by converting it to hesperetin-7-O-glucoside (Hes-7-G) using Aspergillus sojae naringinase. They also found that Hes-7-G had greater antibacterial activity against Helicobacter pylori than hesperetin, but similar activity to hesperidin; the lower inhibitory effect of hesperetin was due to its poor water solubility, and the antibacterial activity of hesperidin and its derivatives depended on the presence or absence of a sugar moiety and the bacteria tested.
Hesperidin has potential antihyperglycemic effects [15,21] by inhibiting α-glucosidase activity [35] and could play a future role in treating type-2 DM. The IC50 values of hesperidin, HG1, HG2 and diosmin were 2.75, 2.48, 2.36 and 2.99 mg/mL, respectively, confirming the greater inhibitory effects of HG1 and HG2 (Ki of 2.20 mM). Sahnoun et al. [35] reported that the IC50 of hesperidin for inhibiting the Saccharomyces cerevisiae α-glucosidase from Sigma (St Louis, Mo, USA) was 111 μM, and that the inhibitory properties of hesperidin, hesperetin, and naringin depended on their structures. For example, the hydroxyl group at 4′-position in the B ring of flavonoids was associated with greater α-glucosidase inhibition than the methoxy group. They found that flavonoids with a 4′-methoxy substitution in the B ring showed the greatest inhibition against α-amylase, followed by 3′-OH-4′-methoxy and 4′-OH substitution; however, this report did not study the effect of the glucose-linked glycoside moiety. Based on computational molecular dynamics and docking simulations, they also found that two hesperetin rings interacted with several residues near the active site on the α-glucosidase, such as Lys155, Asn241, Glu304, Pro309, Phe311, and Arg312, resulting in reversible inhibition of this enzyme in a slope-parabolic mixed-type manner [36].
Od-ek et al. [21] reported that hesperidin decreased goblet cells in diabetic rats when compared to a control group. Goblet cells in the brush border of the small intestinal produce and maintain the protective mucus blanket [37]. Therefore, decreasing the number of goblet cells may lead to a reduction in digestion and absorption in the small intestine and is another potential antihyperglycemic effect of hesperidin. In this work, the standard acarbose, being a well-known α-glucosidase inhibitor, had an IC50 value of 1.64 mg/mL. Kim et al. [38] reported that acarbose is a competitive inhibitor of the rat intestinal α-glucosidase with a Ki of 0.059 μM by using maltose as the substrate. Thus, one of the potential therapeutic approaches to decrease blood glucose and potentially treat type 2 DM is to reduce glucose absorption by inhibiting carbohydrate-hydrolyzing enzymes, e.g., α-amylase, α-glucosidase, and maltase [39]. For hesperidin derivatives, they presented as potential new alternative agents for treating α-glucosidase-associated type 2 diabetes based on their α-glucosidase inhibition. However, more detailed study in this field is required.

Chemicals
Hesperidin and diosmin were purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). The commercial glucose oxidase kit was obtained from Human mit-diagnostics GmbH (Idstein, Germany). Tryptone and yeast extract were obtained from Difco (Bacton Dickinson and company, Sparks, MD, USA). All other chemicals used were of analytical grade and purchased from Sigma-Aldrich (St Louis, MO, USA).

Synthesis and Detection of Hesperidin Glycosides
The p19bBC cells containing CGTase gene from B. circulans A11 were used to prepare recombinant CGTase in the synthesis of the HG products. HG synthesis was carried out using 200 U/mL of CGTase, 1.5% (w/v) β-CD as the glycosyl donor, and 2% (w/v) hesperidin solution containing 80% (v/v) DMSO as the glycosyl acceptor in 0.2 M acetate buffer, pH 5.0 at 60 °C for 6 h [5]. The obtained HG products were detected by thin layer chromatography (TLC) and high performance liquid chromatography (HPLC) [6].

Production and Product Purification
The production and purification of HGs were performed under the optimized conditions described above. The transglycosylation volume of synthesis was scaled up to 300 mL. The reaction mixture was filtered through a 0.45 μm nylon membrane before injecting the 900 μL mixture into preparative HPLC and eluted with acetonitrile:water (20:80 v/v), using a flow rate of 20 mL/min. Each HG peak was separately collected by preparative HPLC (Agilent Technologies 1260, Böblingen, Germany) using a 7 μm ZORBAX SB-C18 PrepHT column (21.2 × 150 mm) and detected with UV detector at 280 nm. Thereafter, the purified HG was concentrated by evaporation for further characterization.

NMR Analysis
The α-1,4-glycosidic bonds of HGs were identified by 1 H and 13 C-NMR spectra with Bruker's AVANCE Tech HD 400 NMR spectrometer (Bruker, Billerica, MA,USA) at the BIOTECH, Thailand. The operation used 400 MHz at room temperature. The NMR spectra were obtained with 3-10 mg of each sample dissolved in DMSO-d6.

Antibacterial Activity
Cultures of the Gram-positive Staphylococcus aureus ATCC 25923 and Gram-negative Escherichia coli ATCC 25922 were used in the study. The microorganisms were grown on Luria-Bertani (LB) broth at 37 °C for 24 h. A broth microdilution method was performed according to the Clinical and Laboratory Standard Institute (CLSI) [41].

Minimal Inhibitory Concentration (MIC)
The MIC value was determined by the microdilution technique in liquid LB medium by incubating the bacteria in LB broth with variable amounts of the sample being tested [26]. This technique utilizes the microdilution method in a 96-well microplate. Briefly, the 0.5 McFarland cultures (OD600 = 0.1) of S. aureus and E. coli were diluted with LB broth in a ratio of 1:200 (v/v). Two-fold serial dilutions of hesperidin, hesperidin glycosides and diosmin were taken 50 μL in 96 well microplates. After 24 h incubation, 1 mg/mL of resazurin solution (10 μL) was added to each well and the plate was then incubated for 2 h. The MIC value was determined as the lowest concentration of the hesperidin, hesperidin glycosides and diosmin that prevented a color change of resazurin. A blue dye (resazurin) becomes pink (resorufin) when it is reduced by oxidoreductase of viable bacteria in a medium. The MIC value was performed in triplicate experiments and the mean presented as mg/mL [42,43].

Minimal Bactericidal Concentration (MBC)
After the MIC test had been completed, the MBC value was investigated by subculturing each well in which no visible growth occurred from a previous MIC test (blue color) to an agar medium. After 24 h incubation at 37 °C, bacterial growth on the agar plate was examined. The lowest concentration at which the hesperidin glycosides eliminated all bacteria represented the MBC. The tests were done in triplicate and the mean MBC was recorded as mg/mL.

In Vitro α-Glucosidase Inhibitory Activity
α-Glucosidase inhibitory activity [44] was performed using intestinal acetone powder from rats (Sigma, St Louis, Mo, USA). Hesperidin, hesperidin glycosides and diosmin inhibitors were dissolved in DMSO and prepared at final concentrations of 0.1-2 mg/mL. Then, 100 U/mL of α-glucosidase were pre-incubated with each inhibitor at 37 °C for 30 min, to which sucrose substrate (5.85 mM) in 0.1 M phosphate buffer (pH 6.9) was added. This was incubated for 10 min at 37 °C. After incubation, the reaction was stopped by boiling for 10 min at 100 °C and 10 μL aliquots from each the reaction were added to 1 mL glucose oxidase reagent and incubated for 5 min at 37 °C. The amount of glucose released from this reaction was measured using a glucose oxidase kit at 500 nm. A control reaction was performed without adding inhibitor and acarbose was used as a standard compound for comparison of α-glucosidase inhibitory activity (positive control). All reactions were performed in triplicate experiments. The percentage of α-glucosidase inhibition was calculated by the following Equation (1) Where Acontrol is the absorbance of the enzymatic reaction without inhibitor, and Asample is the absorbance of the enzymatic reaction in presence of the inhibitor. The IC50 was calculated by the curve fitting the data (GraphPad Prism 5.0, GraphPad Software, Inc., San Diego, CA, USA).
Initial rates of reaction were measured at different sucrose concentrations. Lineweaver-Burk plots, 1/V0 vs. 1/[sucrose] with/without HG2 α-glucosidase inhibitor were generated using the Sigma Plot 9.0 program (Systat Software Inc., San Jose, CA, USA) to generate the maximum reaction rate (Vmax) and the Michaelis constant (Km). The inhibition constant (Ki) was calculated using the following Equation (2) [45,46]: where [I] is the inhibitor concentration.

Statistical Analyses
Data are presented as the mean ± SD from three independent experiments. Statistical analysis was performed using a Student's t-test with IBM SPSS Statistic version 26.0 (SPSS Corporation, Chicago, IL, USA). A P-value < 0.05 was considered to be statistically significantly.

Conclusions
In conclusion, our structural investigation and upscale production of hesperidin derivatives in the presence of sugar moiety was successfully carried out. Hesperidin glycosides (HG1-2) were shown to be able to prevent bacterial growth and inhibit intestinal α-glucosidase. The best IC50 of α-glucosidase inhibition was acarbose, followed by HG2 and HG1. The present study supports the potential use of HGs as antibacterial and anti-αglucosidase (antidiabetic) agents.