Advances in Recombinant Lipases: Production, Engineering, Immobilization and Application in the Pharmaceutical Industry

: Lipases are one of the most used enzymes in the pharmaceutical industry due to their e ﬃ ciency in organic syntheses, mainly in the production of enantiopure drugs. From an industrial viewpoint, the selection of an e ﬃ cient expression system and host for recombinant lipase production is highly important. The most used hosts are Escherichia coli and Komagataella pha ﬃ i (previously known as Pichia pastoris ) and less often reported Bacillus and Aspergillus strains. The use of e ﬃ cient expression systems to overproduce homologous or heterologous lipases often require the use of strong promoters and the co-expression of chaperones. Protein engineering techniques, including rational design and directed evolution, are the most reported strategies for improving lipase characteristics. Additionally, lipases can be immobilized in di ﬀ erent supports that enable improved properties and enzyme reuse. Here, we review approaches for strain and protein engineering, immobilization and the application of lipases in the pharmaceutical industry.


Introduction
Lipases have a myriad of industrial and biotechnological applications that rely on the variety of reactions that they catalyze. Based on hydrolytic reactions these biocatalysts can be applied: in the food industry for lipid modification through complete or partial triacylglycerol degradation, resulting in food with improved sensorial properties [1,2]; as a digestive auxiliary [3]; being incorporated in detergents, facilitating the removal of fat stains from fabrics. Through synthetic reactions, lipases can be applied to biodiesel production since they catalyze the transesterification of vegetable oils and simple alcohols [4]. On the other hand, one of the most relevant lipase applications is in the pharmaceutical industry where, through hydrolytic or esterification reactions, lipases can discriminate enantiomers from It was estimated that using continuous mode, CALB production was almost six times greater than with the fed-batch after six weeks of cultivation. Figure 1 illustrates sequential approaches for recombinant lipase production.
Catalysts 2020, 10, x FOR PEER REVIEW 5 of 33 the fed-batch after six weeks of cultivation. Figure 1 illustrates sequential approaches for recombinant lipase production.

Prokaryotic Expression Systems
E. coli is by far the most used prokaryotic microorganism for recombinant production of lipases, followed by the Gram-positive soil bacterium B. subtilis. Hence, according to Borrelli and Trono [42], E. coli represents 50% of the total heterologous production of lipases by eukaryotic and prokaryotic hosts. This dominant position in the production hierarchy has been earned by the more than 40 years of intensive use as production host and the even longer record of serving as one of the most important biological model organisms. As a result, E. coli is likely the best understood organism on the planet with a superior genetic toolbox and profound insights into its genetics and physiology. Together, these advantages have been used to construct highly efficient E. coli based cell factories for heterologous protein production, and there are examples where the new protein constitutes 30% of the total intracellular content of proteins. Moreover, E. coli based processes are typically easy to manipulate and scale-up due to consolidated methodologies of fermentation, cloning and expression with different strains, protocols and studies available. Many strategies are available to optimize a production process. For example, in the context of recombinant lipases, purification can be simplified by fusing the lipase gene to sequences encoding signal peptides (PelB and OmpA) as they will direct the proteins to the periplasmic space. Moreover, yields of soluble protein may be increased by coexpression with chaperones (GroES, GroEL and ClpB). In the first case, the enzyme could be recovered by ultrasonication or other cell lysis processes. The co-expression with chaperones enables the correct folding of the lipase, increases its solubility and decreases protein aggregation. Molecular chaperones are included in a family of unrelated classes of protein that mediate the correct assembly of other polypeptides-i.e., correct folding [66].
The major limitations of using E. coli as an expression host include its inability to secrete large amounts of proteins or to perform post-translational modifications (PTMs)-i.e., protein glycosylation. However, E. coli is a suitable host organism for bacterial lipases, such as from the genera Pseudomonas, Bacillus, Staphylococcus, Serratia, Burkholderia, and from metagenomic libraries. Furthermore, E. coli has been explored in studies of the biochemical characterization of lipases produced by previously identified microorganisms [63,67] or uncultured strains discovered by metagenomics [68,69]. Table 1 shows different lipase sources, prokaryotic expression systems and lipase properties.
Commercial lipases are mostly obtained from mesophilic organisms that grow at temperatures between 20 and 45 °C. However, extremophile microorganisms-psychrophiles (<20 °C) and thermophiles (>40 °C)-have drawn attention as natural lipase sources due to the outstanding

Prokaryotic Expression Systems
E. coli is by far the most used prokaryotic microorganism for recombinant production of lipases, followed by the Gram-positive soil bacterium B. subtilis. Hence, according to Borrelli and Trono [42], E. coli represents 50% of the total heterologous production of lipases by eukaryotic and prokaryotic hosts. This dominant position in the production hierarchy has been earned by the more than 40 years of intensive use as production host and the even longer record of serving as one of the most important biological model organisms. As a result, E. coli is likely the best understood organism on the planet with a superior genetic toolbox and profound insights into its genetics and physiology. Together, these advantages have been used to construct highly efficient E. coli based cell factories for heterologous protein production, and there are examples where the new protein constitutes 30% of the total intracellular content of proteins. Moreover, E. coli based processes are typically easy to manipulate and scale-up due to consolidated methodologies of fermentation, cloning and expression with different strains, protocols and studies available. Many strategies are available to optimize a production process. For example, in the context of recombinant lipases, purification can be simplified by fusing the lipase gene to sequences encoding signal peptides (PelB and OmpA) as they will direct the proteins to the periplasmic space. Moreover, yields of soluble protein may be increased by co-expression with chaperones (GroES, GroEL and ClpB). In the first case, the enzyme could be recovered by ultrasonication or other cell lysis processes. The co-expression with chaperones enables the correct folding of the lipase, increases its solubility and decreases protein aggregation. Molecular chaperones are included in a family of unrelated classes of protein that mediate the correct assembly of other polypeptides-i.e., correct folding [66].
The major limitations of using E. coli as an expression host include its inability to secrete large amounts of proteins or to perform post-translational modifications (PTMs)-i.e., protein glycosylation. However, E. coli is a suitable host organism for bacterial lipases, such as from the genera Pseudomonas, Bacillus, Staphylococcus, Serratia, Burkholderia, and from metagenomic libraries. Furthermore, E. coli has been explored in studies of the biochemical characterization of lipases produced by previously identified microorganisms [63,67] or uncultured strains discovered by metagenomics [68,69]. Table 1 shows different lipase sources, prokaryotic expression systems and lipase properties.
Commercial lipases are mostly obtained from mesophilic organisms that grow at temperatures between 20 and 45 • C. However, extremophile microorganisms-psychrophiles (<20 • C) and thermophiles (>40 • C)-have drawn attention as natural lipase sources due to the outstanding properties of their enzymes, especially related to thermal stability, pH and tolerance to high salt concentrations [44,70]. These lipases might be identified and characterized through the construction of metagenomics libraries, and heterologous expression, followed by screening. Activity assays are also conducted in the presence of detergents, organic solvents and enzyme inhibitors to evaluate their industrial potential as biocatalysts. Sahoo and collaborators [68], for example, have investigated the metagenomics of samples harvested from hot springs (Table 1). After the selection of the best clones, the lipase RK-lip479 was cloned and expressed in the strains E. coli DH5α and E. coli BL21 (DE3), respectively. The lipase was expressed intracellularly, had maximum activity at 65 • C and pH 8.0, and maintained 89, 92 and 60% of its activity after 6 h at 55, 65 and 75 • C [68]. Similarly, the thermostable lipase BaG7Lip from B. amyloliquefaciens G7 was isolated from hot spring samples, cloned and expressed in E. coli BL21-Star (DE3) ( Table 1) [71]. In that study, the lipase gene without its signal peptide sequence was fused to a His-tag, purified from the intracellular environment, and showed optimal temperature at 50 • C and pH 8.0. An activity improvement of 15% was observed with 30% acetone and glycerol [71].
Salwoom and collaborators [72] conducted a study of expression and biochemical characterization of a recombinant lipase from a previously isolated Pseudomonas sp. strain from Antarctica ( Table 1). The lipase LSK25 was cloned and expressed in E. coli BL21 (DE3). The enzyme was stable in a range of 5-30 • C and pH 6-8. Among the several metal ions evaluated, only Ca 2+ ions increased the lipase activity. Supplementation of 1 and 3-5 mM Ca 2+ enhanced the lipase activity in 50 and 150%, respectively, confirming the activation of this lipase by Ca 2+ ions, as happens with other cold-adapted lipases as well [72]. The lipase LSK25 activity was further boosted up to three times in organic solvents, namely toluene, xylene, n-hexane, n-heptane and n-hexadecane. Higher activities were shown for long chain fatty acids contained in coconut oil and rice bran oil, and also for pNP-C12 synthetic ester. The authors proposed the application of the lipase LSK25 in the food, agrochemical and pharmaceutical industries [72].
The co-expression of the lipase gene with chaperones could be a reliable tool for obtaining the overproduction of recombinant lipases. Alnoch and collaborators [73], for example, co-expressed the lipase LipBC and its foldase LifBC genes from the Gram-negative bacterium Burkholderia contaminans LTEB11 (Table 1). E. coli BL21 (DE3) strain was used as expression host and the best lipolytic activity was verified with the co-expression of the N-terminal truncated lipase gene with the full-length foldase gene. This combination resulted in an activity of 127 U/mL against olive oil. Interestingly, no lipolytic activity was found when the lifBC gene was not expressed, showing that this foldase plays a key role in lipase conformation and function [73]. Although only the lipBC gene was fused to a His-tag, both proteins were purified in a single affinity chromatography step, which could be attractive from an industrial perspective. The foldase LifBC must have formed a complex with the lipase LipBC, which was bound to the column, through a hydrophobic interaction between them. The complex formed, rLipBC, had maximum specific activity of 1426 U/mg towards tributyrin, and maintained a high conversion rate (>80%) of ethyl-oleate in n-hexane over five reaction cycles of 6 h at 45 • C, when it was immobilized on a hydrophobic support (Sepabeads™ FP-BU) [73]. Activity enhancement with Ca 2+ ions; activity boosted in toluene, xylene, n-hexane, n-heptane and n-hexadecane; higher activities towards long chain fatty acids contained in coconut oil and rice brain oil, and pNP-C12.

Eukaryotic Expression Systems
The main studied and used eukaryotic expression systems are the yeasts K. phaffii, S. cerevisiae, Y. lipolytica, and the species of filamentous fungus Aspergillus that represent nearly 35, 8, 1 and 3% of all heterologously produced lipases, respectively [42]. These microorganisms are not only efficient producers, but some have the status of being generally recognized as safe (GRAS) (S. cerevisiae, Y. lipolytica, A. niger and A. oryzae). Proteins produced in eukaryotic cells can undergo a series of PTMs that can affect their chemical and physical properties [82]. Such modifications can increase protein solubility, stability and secretion rate. In industry, fungal lipases have gained attention because of their stability under different conditions and substrate specificity. Moreover, extraction procedures are simpler in comparison to bacterial lipases due to their extracellular character [34]. Table 2 summarizes different eukaryotic sources of lipases, expression systems and properties.  K. phaffii is a methylotrophic yeast, which is able to express and produce lipases under the control of methanol-responsive alcohol oxidase promoters. It naturally secretes low amounts of proteins in the extracellular medium, but high levels of secreted recombinant lipase can be achieved using methanol as an inducer. This special characteristic makes the lipase purification step easier and cost-effective. In addition, K. phaffii has a number of advantages in comparison to higher eukaryotes. It is a single-celled microorganism, which is easy to genome engineer and it only requires simple culture conditions. It performs many PTM modifications typical for higher eukaryotes, such as disulfide bond formation, glycosylation, proteolytic processing and appropriate folding. Consequently, it can result in a higher degree of biological active lipases in comparison to bacteria [93]. Compared to S. cerevisiae, by using K. phaffii as the production host, the enzyme can be produced in the intracellular space, secreted to the extracellular broth or displayed at cell surface with a reduced hyperglycosylation level [42,43,50]. Moreover, in comparison to S. cerevisiae, K. phaffii can be produced in higher cell densities than S. cerevisiae, resulting in a higher protein productivity. K. phaffii also has low nutritional requirements because it is adapted to catabolize raw carbon and nitrogen sources. Those properties can potentially reduce production costs since these nutrient sources can be found in many industrial by-products. There are several reported strategies to increase the production and secretion of different lipases in K. phaffii. Examples and descriptions of some of the most applied strategies are presented in the paragraphs below.
Promoters play an important role in the first step of setting up a cell factory for effective production of a desired protein. Different types of promoters, such as inducible and constitutively active ones, are available for heterologous gene expression in K. phaffi [94]. One of the most commonly used promoters is the inducible alcohol oxidase 1 gene promoter (pAOX1). The enzyme expressed by the pAOX1 is responsible for the conversion of methanol to formaldehyde, which is the first reaction in the metabolism of methanol. The promoter is tightly regulated by the presence of repressing carbon sources, such as glucose, glycerol and ethanol. To become fully activated, it requires the presence of methanol [95]. The pAOX1-based protein expression system can be easily applied to control bioreactor cultivations. The tight regulation by the repressing carbon source can enable cell growth to high densities prior to heterologous protein production. However, on an industrial scale this system is hazardous because methanol is a flammable chemical. For that reason, there have been attempts to identify or create promoters that achieve equal or even better protein yields [94,96]. Another inducible promoter that has shown promising results is the formaldehyde dehydrogenase 1 gene promoter (pFLD). The formaldehyde dehydrogenase 1 enzyme is involved in both methanol metabolism and in the assimilation of some amines (ex. methylamine) as a nitrogen source. pFLD can be activated by methanol or methylamine and, similar to pAOX1, be repressed by some carbon sources. Cultivations using sorbitol as carbon source and methylamine as nitrogen source demonstrated that sorbitol did not repress the expression of enzymes involved in methanol metabolism, as formaldehyde dehydrogenase 1 [87].
It should be considered that inducible promoters require longer fermentation runs due to the induction step. Therefore, constitutive promoters may serve as a tool to create more cost-effective fermentation cultivations. One promoter that has been applied successfully to lipase production is the glyceraldehyde-3-phosphate dehydrogenase promoter (pGAP). Since it is a strong constitutive promoter, no induction step is required, resulting in simpler and shorter fermentation cultivations. The expression of Fusarium solani NAN103 lipase (FSL) under pGAP or pAOX1 showed that pGAP-FSL produced the highest specific lipase activity and, in addition, there was a two-day reduction in the cultivation time [88]. Many promoters with different strengths and properties are available for expression in K. phaffii. Testing different types of promoters for constructing a specific protein cell factory is a good starting point for the further development and optimization of the heterologous expression system. With respect to lipase production, a promoter that gives good yields for one enzyme will not necessarily give the best results for another [92].
In most cases, increasing the copy number of a desired gene enhances the expression of the recombinant protein. In K. phaffi, the production of the lipase CALB was improved 2.3 fold by increasing the copy number to three. In this experiment, a defective marker approach was applied based on the leucine marker leu2-d, in which multiple copies were necessary to reach a state of prototrophy [65]. However, in some cases a high gene dosage can negatively affect the production because the secretory pathway may become overwhelmed by the effects of high levels of recombinant protein that need to be correctly folded [97]. When R. oryzae ROL was expressed in up to eight copies, the best results were achieved with the strain harboring five copies, with a fold increase of eight. Since strains harboring more copies produced lower levels of ROL, these strains may have potentially produced unfolded proteins that lead to ER stress and the activation of the unfolded protein response (UPR) [86]. Therefore, it is important to adjust the copy number by taking into account the protein folding capacity of the host.
Even though K. phaffii has the potential to fold lipases properly, the native system's secretory pathway may reach its full capacity. This might result in the accumulation of unfolded proteins in the endoplasmic reticulum (ER) that will trigger the activation of UPR. In this case, the UPR functions by reducing the translation of proteins, degrading misfolded proteins and increasing the production of molecular chaperones involved in protein folding. There are many different types of chaperones with various refolding and/or stabilization properties [98]. As an example, protein disulfide isomerase (PDI) catalyzes the formation and breakage of disulfide bonds between cysteine residues, permitting proteins to achieve their correct folding. Other chaperones, such as Kar2p, also known as binding immunoglobulin protein (BIP) in mammals, play key roles in protein translocation through the ER. BIP/Kar2p stabilizes immature proteins by binding to their hydrophobic amino acids, allowing the protein to achieve its correct folding. Moreover, BIP/Kar2p prevents unfolded protein aggregation by assisting in ER-associated degradation (ERAD).
The overexpression and co-overexpression of chaperones in K. phaffii have been demonstrated to improve protein secretion and production yield [99]. The co-expression of PDI increased the production of the MAS1 lipase from marine Streptomyces 1.7 fold. MAS1 was expected to have two pairs of disulfide bonds and the higher levels of PDI were predicted to assist their formation [83]. Interestingly, increasing the copy number of PDI does not always automatically increase the protein production and secretion. Rhizopus chinensis lipase r27RCL was co-expressed with different copy numbers of PDI in K. phaffii. PDI was expected to ease bottlenecks in the ER, since the crystal structure of the lipase r27RCL revealed that it contains three disulfide bonds. One copy number of PDI resulted in a one fold increase in enzyme activity, whereas two, three and four copies resulted in lower activity. The higher copy number of PDI may reduce the availability of transcription and translation machinery for the expression of the lipase gene [84].
The overexpression of different combinations of chaperones has also resulted in an increase in lipase production. The production of the lipase CALB from C. antarctica was tested with the individual overexpression of different chaperones (Ydj1p, Ssa1p, Sec63p, and Kar2) and with a combination of them. The chaperones Ssa1p and Ydj1p stabilize the nascent peptide chain and help its translocation into the ER. Sec63p binds to the translocation peptide and prevents it from moving backwards through the ER channel. The best results were achieved with the combination of Ydj1p-Ssa1p, increasing the production by 2.5 fold. This suggests that these chaperones interact synergistically by enhancing protein folding, secretion and preventing protein aggregation. In contrast, a decrease in CALB was observed with the expression of Kar2p. This could indicate that the excessive presence of Kar2p shifted the ER balance towards the ERAD pathway, resulting in decreased CALB secretion [85].

Alternative Eukaryotic Hosts
Different hosts can provide a range of diverse properties, which may be better suited to a specific cell factory design. For that reason, a lot of effort has been made in recent years to find other eukaryotic hosts for lipase production. The yeast Y. lipolytica is considered a suitable production host because of its expanding genetic tools for heterologous expression, and its ability to metabolize raw substrate and secrete protein in large amounts [100]. The comparison of CALB production by Y. lipolytica and K. phaffii was performed by using well-characterized integration targets for both strains -the leu2 locus in Y. lipolytica and the his4 locus in K. phaffii. CALB was expressed in Y. lipolytica under the control of erythritol inducible promoter pEYK3AB and in K. phaffii under the methanol inducible pAOX1. The results showed that, in Y. lipolytica, growth rate and detected CALB activity were higher than those achieved in K. phaffii. The difference in growth rate has been suggested to be due to the loss of some of the carbon source methanol, which can be converted directly to CO 2 instead of biomass production in K. phaffii. Interestingly, even though enzyme activity was higher in Y. lipolytica, the mRNA expression levels of CALB encoding gene in K. phaffii were higher than in Y. lipolytica. Higher CALB stability with suitable secretion levels may explain the better performance of Y. lipolytica [89].
The filamentous fungus A. niger represents an interesting alternative host because of its high production yield of extracellular enzymes, non-toxicity, robustness and GRAS status [101]. However, there are indications that lipase might not be secreted properly by A. niger. As an example, the production of a lipase from T. lanuginosus (accession no. AF054513) resulted in only 1% of A. niger total cellular protein because most of the lipase produced was attached to the mycelium [90].
Another promising fungal host for lipase production is Trichoderma reesei, with potential for high protein secretion and a well-established fermentation platform. It also has a well-characterized genetic toolbox and transformation can be achieved using selection markers and by stable integration into the genome [102]. A drawback encountered with T. reesei as a lipase production host is that it produces a high level of cellobiohydrolase I (CBHI) that represents approximately 50% of all proteins secreted. The reduction in the unnecessary production of CBHI could result in improved levels of heterologous expression. The RNAi mediated gene silencing of cbhI improved the production of the lipase lip from A. niger in T. reesei, with an increase up to 3.2 fold. The reduction in CBHI enhanced the availability of the protein folding and secretion machinery. Moreover, this strategy allowed the host to still have CBHI present, thereby keeping the strain in a more natural state [91].

Structural Characteristics of Lipases
Knowing and understanding the structure of an enzyme is of key importance not only to improve enzyme production, but also mainly to provide information for protein engineering through site directed mutagenesis. Regarding lipase structural features, they consist of a catalytically active core domain (the α/β-hydrolase fold), similar to other hydrolases, containing the catalytic triad and the oxyanion hole. They might also contain additional structural modules, such as a lid or flap-like structure. Most of the lipases possess an active core domain predominantly composed of eight parallel β strands forming a super-helically twisted central β sheet surrounded by a varying number of α helices [103,104]. However, the number, as well as the organization of β strands, may still diversify. For instance, Bacillus subtilis lipase lacked the β1 and β2 strands in the canonical fold [105]. In addition, the first X-ray structure of a triglyceride lipase (Mucor miehei) was reported by Brady et al. [106].  The catalytic mechanism of lipases is based on a catalytic triad made up of a nucleophile amino acid (Ser), an acid (Asp or Glu), and a His. The catalytic triad containing Asp is known in enzymes, such as proteases. Interestingly, however, different from the other lipases and serine proteases, the catalytic triad of a G. candidum lipase is Ser-His-Giu, with glutamic acid replacing the common aspartate [107]. The central nucleophile residue is located within a conserved Glu-X-Ser-X-Glu motif [108]. The oxyanion hole is another important component involved in the catalytic efficiency of this enzyme, since it helps in stabilizing the transition state in the catalysis. During the catalytic process, a negatively charged tetrahedral intermediate is generated, and the oxygen ion thus formed is stabilized by the oxyanion hole amino acids. The oxyanion hole residues play an essential role in stabilizing this oxygen by hydrogen bonding. The catalytic region of lipases has one of the oxyanion hole's residues positioned adjacent to the nucleophile amino acid serine, while the second residue is located between the β3 strand and α helix [104]. Lipases also possess a lid or flap-like structure, which is composed of one or more α helices of variable length. The binding pocket of lipases is present on the central β sheet, which can be a hydrophobic, crevice-like binding site located near the protein surface or funnel-like or tunnel-like binding sites. The lid domain involves specific interactions with the substrate and controls the inactive/active-form enzyme equilibrium [33].

Lipase Engineering
Protein engineering is the process of customizing new enzymes with improved features by altering their primary amino acid sequences. Given the considerable diversity of possible alterations, this procedure has already produced remarkable results in the design of optimized lipases used in important industrial areas. Protein engineering strategies, such as rational design and directed evolution have led to interesting results in the improvement of different lipases.
Engineering a protein by rational design uses prior knowledge of protein structure and in-depth computational modeling approaches to deliberately design new biocatalysts [109]. In part, the number of lipase structures deposited in the Protein Data Bank (PDB, https://www.rcsb.org/) and lipase sequences information in various databases has substantially facilitated the rational design of these proteins. Furthermore, many different types of modeling software have been created, which makes this methodology easier to use and enhances the success rate of modeling predictions. Usually, The catalytic mechanism of lipases is based on a catalytic triad made up of a nucleophile amino acid (Ser), an acid (Asp or Glu), and a His. The catalytic triad containing Asp is known in enzymes, such as proteases. Interestingly, however, different from the other lipases and serine proteases, the catalytic triad of a G. candidum lipase is Ser-His-Giu, with glutamic acid replacing the common aspartate [107]. The central nucleophile residue is located within a conserved Glu-X-Ser-X-Glu motif [108]. The oxyanion hole is another important component involved in the catalytic efficiency of this enzyme, since it helps in stabilizing the transition state in the catalysis. During the catalytic process, a negatively charged tetrahedral intermediate is generated, and the oxygen ion thus formed is stabilized by the oxyanion hole amino acids. The oxyanion hole residues play an essential role in stabilizing this oxygen by hydrogen bonding. The catalytic region of lipases has one of the oxyanion hole's residues positioned adjacent to the nucleophile amino acid serine, while the second residue is located between the β3 strand and α helix [104]. Lipases also possess a lid or flap-like structure, which is composed of one or more α helices of variable length. The binding pocket of lipases is present on the central β sheet, which can be a hydrophobic, crevice-like binding site located near the protein surface or funnel-like or tunnel-like binding sites. The lid domain involves specific interactions with the substrate and controls the inactive/active-form enzyme equilibrium [33].

Lipase Engineering
Protein engineering is the process of customizing new enzymes with improved features by altering their primary amino acid sequences. Given the considerable diversity of possible alterations, this procedure has already produced remarkable results in the design of optimized lipases used in important industrial areas. Protein engineering strategies, such as rational design and directed evolution have led to interesting results in the improvement of different lipases.
Engineering a protein by rational design uses prior knowledge of protein structure and in-depth computational modeling approaches to deliberately design new biocatalysts [109]. In part, the number of lipase structures deposited in the Protein Data Bank (PDB, https://www.rcsb.org/) and lipase sequences information in various databases has substantially facilitated the rational design of these proteins. Furthermore, many different types of modeling software have been created, which makes this methodology easier to use and enhances the success rate of modeling predictions. Usually, the information derived from computer modeling identifies certain amino acids (hot-spots) that should be altered to lead to a change in the lipases' properties. By using computational predictions, Mohammadi et al. [110] constructed four mutants of S. marcescens lipase. The MutG2P and MutG59P mutants showed higher catalytic efficiency and thermal stability than the wild-type (WT) enzyme. Furthermore, other computational approaches have been developed to predict the effects of mutations on lipases. Li et al. [37] used a strategy denoted as RIF that consists of three computational methods-i.e., Rosetta ddg monomer, I Mutant 3.0, and FoldX-to rationally design and optimize the thermal stability of R. miehei lipase. The most stable mutant, harboring the mutations T18K/T22I/E230I/S56C-N63C/V189C-D238C, exhibited a 14.3 • C higher melting temperature and a 12.5 fold increase in half-life at 70 • C. Kim et al. [111] selected different mutants of CALB based on the B-factor value of amino acid residues and computational modeling by RosettaDesign. The melting temperature of the mutant R249L was increased by 2.3 • C compared to that of WT CALB. Moreover, the rational strategy was applied to block the primary acyl-binding tunnel of CALA lipase to create variants with high specificity for medium-chain-length fatty acids [112]. Likewise, rational design was used to engineer CALA, aiming to develop a highly selective variant for trans-fatty acids [113,114].
The dynamic behavior of lipases in different conditions has also been investigated using computational approaches, such as molecular dynamics (MD) simulations. The MD simulations have made progress in this direction since they provide atomistic information about the dynamic molecular interactions, which determine the protein stability and function [115]. Haque and Prabhu [116] carried out MD simulations of a mutant porcine pancreatic lipase in open and closed conformations using different solvents to explain the dynamics of lid opening. At higher temperatures, it was possible to observe the lid opening by the mutants Asp250Val and Glu254Leu, suggesting the important role of these residues in holding the lid in closed conformation, which can affect the activity of this enzyme. A computational design scheme based on MD simulations was also applied to improve the thermal stability of the lipase LIP2 from Y. lipolytica [117]. Based on the four structural parameters RMSD, Rg, SASA and the number of internal hydrogen bonds, it was found that the V213P mutant would have higher thermal stability than its WT parent. V213P also has an optimal temperature of 42 • C, which is 5.0 • C higher than that of the wild type.
Directed evolution (also called molecular evolution) does not require that the amino acid sequence and three-dimensional structure should be previously available, as random mutagenesis generates libraries with random mutations in the gene of interest. Those random mutations can be induced by error-prone PCR, chemicals, irradiation, DNA shuffling or staggered extension process (StEP). Owing to the high number of possible clones and enzyme variants, it is necessary to conduct high-throughput and time-consuming screening for the selection of the best enzyme variants created. Besides rational design and directed evolution, there is a third approach for engineering an enzyme to acquire better properties. Semi rational design is the combination of the former two approaches. It can be summarized as the generation of libraries of mutant genes via the saturation mutagenesis method. In this technique, one or a few amino acid residues are replaced by the other 19 amino acid possibilities through degenerate codons. Semi rational design has been proven to be a rapid laboratory method for engineering proteins, once a relatively smaller size of libraries are generated, facilitating the further screening and selection step [50,118,119].
Curiously, Alfaro-Chávez and collaborators [76] scrutinized how many changes are necessary to transform a non-attractive lipase into an enzyme with better properties and potential for use in industrial applications. Lip3-encoding gene from Drosophila melanogaster was cloned into the vector pETMCSIII and expressed in the E. coli BL21 (DE3) system. Concomitantly, error-prone PCR was used to generate libraries of mutant variants harboring random mutations along the lipase gene. After seven rounds of directed evolution, screening and selection, only five mutant lipases stood out from tens of hundreds of lipase variants. The best variant, R7_59A, had eight substitutions in its sequence compared to the WT lipase. The lipase R7_59A showed an activity 4, 5, 6 and 12 fold higher than WT towards coconut oil, tributyrin, glyceryl trioctanoate, and glyceryl trioleate substrates, respectively. Using crude lysate, there was an improvement of 228 times in the 57_59A activity against pNP-C8 compared to the WT. Furthermore, the 57_59A mutant had a 6.3, 1.5, 7.8 and 2.6 fold increment in the activity against pNP-C3, pNP-C8, pNP-C16 and pNP-C18 substrates compared to WT. The induced mutations in the best five variants were present in amino acid residues related to the cap domain stability, residues near to the catalytic triad and in residues of the hinge points of the cap domain. According to the study, those lipase variants could be targeted in further evolutions aiming to use them in detergent formulation [76].

Relevant Immobilization Methods for Recombinant Lipases
The use of enzymes in various industrial areas has significantly increased due to several associated benefits. However, some factors, such as low enzymatic stability, may limit the use of lipases and other industrial enzymes [120,121]. One of the most important and widely used techniques to improve enzyme properties is their immobilization, in which the biocatalysts are attached to an inert support. The immobilization of enzymes provides several benefits, such as (1) the production of efficient and stable biocatalysts; (2) possibility of recovery and reusability of enzymes [122,123]; (3) easy purification of the products when compared to the free enzymes; (4) increasing the efficiency; (5) permitting their applications in continuous fixed-bed operation [122].
The immobilization methods can be separated into two classes-namely, the physical and chemical methods. Physical methods include support and enzyme interactions of different types, including hydrogen bonds and hydrophobic interactions. In the latter method, the formation of covalent bonds can be achieved through amide, ether, thio-ether or carbamate bonds between the support and the enzyme [124][125][126]. The physical methods specifically include entrapment and adsorption, while chemical methods include cross-linking and covalent binding. Different enzyme immobilization methods are grouped and listed in Figure 3.
Catalysts 2020, 10, x FOR PEER REVIEW 18 of 33 compared to the WT. Furthermore, the 57_59A mutant had a 6.3, 1.5, 7.8 and 2.6 fold increment in the activity against pNP-C3, pNP-C8, pNP-C16 and pNP-C18 substrates compared to WT. The induced mutations in the best five variants were present in amino acid residues related to the cap domain stability, residues near to the catalytic triad and in residues of the hinge points of the cap domain. According to the study, those lipase variants could be targeted in further evolutions aiming to use them in detergent formulation [76].

Relevant Immobilization Methods for Recombinant Lipases
The use of enzymes in various industrial areas has significantly increased due to several associated benefits. However, some factors, such as low enzymatic stability, may limit the use of lipases and other industrial enzymes [120,121]. One of the most important and widely used techniques to improve enzyme properties is their immobilization, in which the biocatalysts are attached to an inert support. The immobilization of enzymes provides several benefits, such as (1) the production of efficient and stable biocatalysts; (2) possibility of recovery and reusability of enzymes [122,123]; (3) easy purification of the products when compared to the free enzymes; (4) increasing the efficiency; (5) permitting their applications in continuous fixed-bed operation [122].
The immobilization methods can be separated into two classes-namely, the physical and chemical methods. Physical methods include support and enzyme interactions of different types, including hydrogen bonds and hydrophobic interactions. In the latter method, the formation of covalent bonds can be achieved through amide, ether, thio-ether or carbamate bonds between the support and the enzyme [124][125][126]. The physical methods specifically include entrapment and adsorption, while chemical methods include cross-linking and covalent binding. Different enzyme immobilization methods are grouped and listed in Figure 3. The immobilization of an enzyme by adsorption is a simple technique with high commercial value due to its simplicity, regular use in large-scale processes, low cost, retention of high enzyme activity, and relatively chemical-free enzyme binding [127]. Supports with different degrees of hydrophobicity were previously used such as butyl Sepabeads and octadecyl Sepabeads [126], decaoctil-Sepabeads [128], macroporous resin HPD826 [129], polypropylene powder [124,130,131] and pore-expanded mesoporous silica (SBA-15) [132]. The use of hydrophobic supports is of particular interest, because these supports mimic the enzymes' natural media and can often promote hyperactivation, highly selective adsorption, purification, increased enantioselectivity, and strong but reversible immobilization, making support reuse possible after the enzyme has been deactivated [133,134].
Hydrophobic core-shell polymeric supports, including Accurel, PMMA/PMMA (poly(methyl methacrylate) on core and shell) and PMMA-co-DVB/PMMA-coDVB (methyl methacrylate copolymerized with divinylbenzene on core and shell) obtained by a combined suspension and The immobilization of an enzyme by adsorption is a simple technique with high commercial value due to its simplicity, regular use in large-scale processes, low cost, retention of high enzyme activity, and relatively chemical-free enzyme binding [127]. Supports with different degrees of hydrophobicity were previously used such as butyl Sepabeads and octadecyl Sepabeads [126], decaoctil-Sepabeads [128], macroporous resin HPD826 [129], polypropylene powder [124,130,131] and pore-expanded mesoporous silica (SBA-15) [132]. The use of hydrophobic supports is of particular interest, because these supports mimic the enzymes' natural media and can often promote hyperactivation, highly selective adsorption, purification, increased enantioselectivity, and strong but reversible immobilization, making support reuse possible after the enzyme has been deactivated [133,134].
Hydrophobic core-shell polymeric supports, including Accurel, PMMA/PMMA (poly(methyl methacrylate) on core and shell) and PMMA-co-DVB/PMMA-coDVB (methyl methacrylate copolymerized with divinylbenzene on core and shell) obtained by a combined suspension and emulsion polymerization process have demonstrated great potential in the immobilization of a recombinant C. antarctica lipase [135]. This technique involves the lipase interfacial activation versus the hydrophobic surface of the support, which is the main cause of the lipase improved activity and stabilization [135,136].
One example of commercial lipase immobilized on a hydrophobic support is Novozym 435 (N435), supplied by Novozymes. The enzyme is expressed in A. niger and produced by interfacial activation of lipase B from C. antarctica on the resin, Lewatit VP OC 1600. This resin is a macroporous support formed by poly(methyl methacrylate) crosslinked with divinylbenzene. N435 is considered one of the most widely used commercial biocatalysts in both academy and industry [137].
In physical adsorption, enzyme leaching is a critical problem that limits the use of the immobilized enzyme in different reaction conditions [138]. To solve this problem, entrapment is employed to restrict the enzyme in the polymer frameworks [139]. In the entrapment method, the enzyme is locked inside the lattice of a polymer matrix or membrane so that it is retained while allowing the penetration of the substrate. That can be achieved with a variety of materials, including polymers, sol-gels, polymer/sol-gel composites and other inorganic materials.
It has been observed that the entrapment of lipases in silica sol-gels increases thermal stability and enhances reaction rates by several orders of magnitude [140]. The entrapment of CALB was successfully achieved with poly 1-vinyl-3-ethylimidazolium bromide (VEImBr) [139], and the immobilization of the whole cells or cell lysates of E. coli, producing a thermostable lipase from T. lanuginosus (rE.coli/lip), was also reported using silica xerogel and nanocarbon in silica composites [141].
For chemical methods, the enzyme is firmly immobilized on the chemically modified support through covalent binding and cross-linking, effectively preventing enzyme leakage. Cross-linking is an improvement on the covalent attachment because the enzyme is cross-linked to the support with the help of a cross-linker. The most commonly used cross-linking reagent is glutaraldehyde, as it is economical and easily obtainable in large quantities [142].
CALB was immobilized by covalent binding on strongly hydrophobic polystyrene microspheres (poly glycidyl methacrylate) activated by epoxy groups, and this immobilization has been proven to play a key role in improving the properties of the lipase [143]. The carrier hollow silica microspheres (MAT540TM) activated by six bisepoxides enclosing different spacers was applied for the immobilization of CALB. The immobilized preparation was used in the kinetic resolution of racemic 1-phenyethanol and five racemic amines using shaken flasks and continuous-flow packed-bed microreactors with good yields and high enantiomeric excess (ee > 99%, for all) [144]. Furthermore, the recombinant CALB expressed in K. phaffii was immobilized by cross-linked enzyme aggregate (CLEA) technology and tested for the synthesis of olvanil [145].
A recombinant lipase from Thermus thermophilus WL expressed in E. coli was immobilized onto the surface, being rich in the amine functional groups (−NH 2 ), of the 3-APTES-modified Fe 3 O 4 @SiO2 nanoparticles [125]. The covalent bond on the aldehyde activated-agarose in the presence of DTT (glyoxyl-DTT-agarose) at pH 7.0 and on glyoxyl-agarose at pH 10.2 was applied for the immobilization of the lipase from Archaeon Pyrococcus furiosus (Pf2001) produced in E. coli B21 [126].
In the last decade, applications of new materials for the immobilization of lipases have drawn special interest. These new material groups include magnetized nano-sized materials [146,147], which provide large surface areas for the attachment of the enzyme and increase the probability of its efficient activity. By using this technique, the improvement of the downstream processing is clear as it facilitates the immobilized enzyme separation from the reaction mixture. Abd El-Aziz et al. [147] immobilized a recombinant Pseudomonas aeruginosa lipase through surface conjugation with gold nanoparticles and observed higher lipolytic activity in the bioconjugate compared to the free enzyme. Dandavate et al. [148] and Yilmaz et al. [149] immobilized a Candida rugosa lipase onto the silica nanoparticles surface and glutaraldehyde-activated aminopropyl glass beads, resulting in easy recovery, and enzyme reuse for ester synthesis. In this context, when Candida antarctica lipase was immobilized on chitosan-coated magnetic nanoparticles of iron activated with glutaraldehyde for the production of the biolubricant ester, the biocatalyst gave half of the initial conversion after seven cycles of esterification [150].
Another special area of interest has been the applications of hybrid and composite materials for lipase immobilization. The hybrid origin materials are synthesized from combinations of precursors of inorganic, organic and mixed inorganic and organic origin and offer high stability, good affinity for enzymes and the presence of many chemical functional groups compatible with the chemical moieties present in the protein structure [133]. An interesting example of a composite material used for enzyme immobilization is the combination of graphene oxide (GO) decorated with ZnO nanoparticles (GO/ZnO). Zhang et al. [151] used this support for the adsorption immobilization of a C. rugosa lipase (CRL) and it exhibited excellent performance in terms of thermal stability and reusability.
A general combined purification and immobilization method, based on modified controlled porosity glass (GPGs) and polymer-coated versions thereof (HybCPGs), which can bind protein affinity tags, named EziG™, was used to immobilize C. antarctica and could be obtained in a significantly shorter time compared to the corresponding Accurel ® -based preparations [152]. Affinity tags are exogenous amino acid residues that selectively bind to a biological or chemical ligand placed on a solid support (e.g., His-tags connected to immobilized Ni) or an immobilized protein partner. Among all the tags, the His-tags are the most used because they are small, less disruptive than other tags and non-immunogenic [153]. The combination of nanotechnology with fusion strategy seems a promising approach and can be devised by using several nanomaterials combined with self-cleaving peptides for one-step, economical, high-yield, and large-scale purification and the immobilization of recombinant proteins [154].

Application of Recombinant Lipases
The versatility of lipases in catalyzing the hydrolysis of esters and reactions of esterification, trans-esterification and inter-esterification, considerably expands the commercial and technological applications of these enzymes. For this reason, they can be applied in the food, oleochemical, pharmaceutical, fine chemical, cosmetics, pulp and paper, leather and biosensor industries and in the treatment of effluents rich in oils and fats [155][156][157]. In addition, they have been used for the synthesis of different oleochemical products, such as free fatty acids, glycerin and its derivatives, biodiesel, biolubricants and different alkyl esters [158].
Despite the vast application potential of the lipases, their application spectrum is limited due to an absence of the process-suitable characteristics necessary to endure harsh processing conditions during industrial applications operating at relatively high temperature and pH conditions. Hence, the lipases envisioned for industrially applicable processes must possess the necessary characteristics, such as stability and high yield, in order to prove their worth. The use of lipases as industrial catalysts depends heavily on the production of recombinant enzymes with biochemical and catalytic characteristics improved by protein engineering. The evolution of strategies to apply these techniques on an industrial scale has enabled a significant reduction in lipase cost, consequently stimulating the development of other industrial applications.
Lipases are commonly applied in the dairy industry for the selective hydrolysis of milk triacylglycerols during cheese ripening. Particularly, a lipase from R. miehei expressed in A. oryzae is commercially available with the trade name Palatase M (Novozymes) and used in the dairy industry to improve cheese properties. Lipase (Est_p6) isolated from a metagenomic library from marine sediments was produced in E. coli and applied to efficiently hydrolyze milk fat and impart a desirable and distinctive flavor to milk products [159]. A Penicillium cyclopium lipase produced in K. phaffii [160] has been used for the synthesis of mono-and diacylglycerols and showed its potential for food emulsifier preparations. The commercial enzyme Novozym 435, an immobilized lipase from C. antarctica expressed in A. niger, has been successfully used to produce sugar esters [161].
Specifically, in the pharmaceutical industry, recombinant enzymes are mainly used for applications as biocatalysts in the synthesis of chiral intermediates for the development of drugs, and as therapeutic drugs for the treatment of various diseases. Lipases offer several advantages compared to other enzymes and several chemical catalysts, such as capacity for the resolution of racemic mixtures by the synthesis of a simple enantiomer being currently exploited for the production of medicines by the pharmaceutical industry, mainly for the manufacturing of antidepressant, antihypertensive and vasodilator drugs [156,162].
Single-enantiomer intermediate synthesis for the preparation of drugs is important in the pharmaceutical industry. Single enantiomers can be produced either by chemical or biocatalytic routes. Biocatalysis is emerging as an efficient alternative to traditional chemical methods for the production of pharmaceuticals. The advantages of the enzyme-catalyzed reactions compared to chemical processes include enantioselectivity and the use of mild reaction conditions which may reduce energy consumption and waste generation.
The following reported examples show that recombinant lipases have been an important tool in improving pharmaceutical processes for chiral drug intermediates and vitamin synthesis.

Pharmaceutical Applications of Recombinant Lipases
Profens (2-arylpropionic acids) are drugs commonly used in the treatment of pain and inflammation associated with tissue injury. The anti-inflammatory activity of ketoprofen, flurbiprofen, ibuprofen, and naproxen is mainly related to their (S)-enantiomer. For these drugs, (R)-enantiomers contribute weakly to cyclooxygenase (COX) inhibition and have been related to side effects-e.g., gastrointestinal pain [163,164].
In recent years, the kinetic resolution of 2-arylpropionic acids by enantioselective esterification has been widely studied. Lipases have been applied in this process and their relevant characteristics demonstrated in many papers; the promising results point to the future applications of these enzymes [5,6,164,165]. For instance, lipases from C. antarctica, C. rugosa [164,166] and A. niger [6] have been applied in the kinetic resolution of profens, making it possible to obtain drugs with high enantiomeric purity-e.g., (R)-flurbiprofen, ee p = 96.3%; E = 90.5%; C = 35.7%), therefore, demonstrating their great potential as biocatalysts in pharmaceutical synthesis. Figure 4 illustrates the kinetic resolution of racemic profens using lipases.
Catalysts 2020, 10, x FOR PEER REVIEW 21 of 33 to other enzymes and several chemical catalysts, such as capacity for the resolution of racemic mixtures by the synthesis of a simple enantiomer being currently exploited for the production of medicines by the pharmaceutical industry, mainly for the manufacturing of antidepressant, antihypertensive and vasodilator drugs [156,162]. Single-enantiomer intermediate synthesis for the preparation of drugs is important in the pharmaceutical industry. Single enantiomers can be produced either by chemical or biocatalytic routes. Biocatalysis is emerging as an efficient alternative to traditional chemical methods for the production of pharmaceuticals. The advantages of the enzyme-catalyzed reactions compared to chemical processes include enantioselectivity and the use of mild reaction conditions which may reduce energy consumption and waste generation.
The following reported examples show that recombinant lipases have been an important tool in improving pharmaceutical processes for chiral drug intermediates and vitamin synthesis.

Pharmaceutical Applications of Recombinant Lipases
Profens (2-arylpropionic acids) are drugs commonly used in the treatment of pain and inflammation associated with tissue injury. The anti-inflammatory activity of ketoprofen, flurbiprofen, ibuprofen, and naproxen is mainly related to their (S)-enantiomer. For these drugs, (R)enantiomers contribute weakly to cyclooxygenase (COX) inhibition and have been related to side effects-e.g., gastrointestinal pain [163,164].
In recent years, the kinetic resolution of 2-arylpropionic acids by enantioselective esterification has been widely studied. Lipases have been applied in this process and their relevant characteristics demonstrated in many papers; the promising results point to the future applications of these enzymes [5,6,164,165]. For instance, lipases from C. antarctica, C. rugosa [164,166] and A. niger [6] have been applied in the kinetic resolution of profens, making it possible to obtain drugs with high enantiomeric purity-e.g., (R)-flurbiprofen, eep = 96.3%; E = 90.5%; C = 35.7%), therefore, demonstrating their great potential as biocatalysts in pharmaceutical synthesis. Figure 4 illustrates the kinetic resolution of racemic profens using lipases. A lipase from the S. marcescens ECU1010 strain produced in E. coli catalyzed the hydrolysis of racemic ketoprofen esters into (S)-ketoprofen, with the highest ee (enantiomeric excess) (91.6%) and yield (48.2%) [168]. A lipase from C. rugosa produced in K. phaffii was used for the kinetic resolution of racemic ibuprofen by means of enantioselective esterification with 74% ee [130]. A lipase from the S. marcescens ECU1010 strain produced in E. coli catalyzed the hydrolysis of racemic ketoprofen esters into (S)-ketoprofen, with the highest ee (enantiomeric excess) (91.6%) and yield (48.2%) [168]. A lipase from C. rugosa produced in K. phaffii was used for the kinetic resolution of racemic ibuprofen by means of enantioselective esterification with 74% ee [130].
Commercial lipase from C. antarctica produced in A. niger and immobilized onto polymethylmethacrylate (Novozym 435) has been efficiently used for the enantioselective esterification of (R,S)-flurbiprofen to obtain the (R)-flurbiprofen methyl ester in 96% ee [166]. A lipase gene from S. marcescens ES-2 was reinserted in the same strain for lipase homologous production and was applied in the enantioselective hydrolysis of (R,S)-flurbiprofen ethyl ester for the production of the optically active form (S)-flurbiprofen [169].
Taking into consideration that the lipase Lip2p from Y. lipolytica has higher catalytic activity than C. rugosa lipases to hydrolyze profen ethyl esters, though its selectivity is not sufficient, Gérard et al. [167] improved Lip2p enantioselectivity by site-directed mutagenesis. The authors identified two amino acid residues (232 and 235) in the lipase hydrophobic substrate binding site as crucial for enantiomer discrimination and enzyme activity. Their results demonstrated the high potential of rational engineering to create biocatalysts suitable for industrial applications.
Chemical synthesis is the common process for obtaining vitamin A derivatives. However, the process presents some issues, such as synthesis steps, which could destroy raw materials (use of acid and alkali), create difficulties in the separation of the final product due to the instability of intermediate products, as well as considerable energy consumption and pollution. Accordingly, a green and environmentally friendly manufacturing method has become the current trend in obtaining vitamin A derivatives. To conveniently prepare the immobilized enzyme for the green synthesis of vitamin A palmitate, T. lanuginosus lipase (TLL) was assembled with apatite derived from calcium phosphate in solution to form TLL@apatite hybrid nanoflowers (hNFs) by mimetic biomineralization. Under the optimized conditions, when vitamin A acetate and palmitic acid were applied as substrates, the yield of the product was up to 90.4% [170].
Recombinant CALB produced by K. phaffii was immobilized and used to synthesize vitamin A palmitate by transesterification of vitamin A acetate and palmitic acid in organic solvent with a conversion ability of 54.3% after 15 cycles [129]. As represented in Figure 5, vitamin A palmitate synthesis could be catalyzed by C. antarctica lipase B.
Catalysts 2020, 10, x FOR PEER REVIEW 22 of 33 Commercial lipase from C. antarctica produced in A. niger and immobilized onto polymethylmethacrylate (Novozym 435) has been efficiently used for the enantioselective esterification of (R,S)-flurbiprofen to obtain the (R)-flurbiprofen methyl ester in 96% ee [166]. A lipase gene from S. marcescens ES-2 was reinserted in the same strain for lipase homologous production and was applied in the enantioselective hydrolysis of (R,S)-flurbiprofen ethyl ester for the production of the optically active form (S)-flurbiprofen [169].
Taking into consideration that the lipase Lip2p from Y. lipolytica has higher catalytic activity than C. rugosa lipases to hydrolyze profen ethyl esters, though its selectivity is not sufficient, Gérard et al. [167] improved Lip2p enantioselectivity by site-directed mutagenesis. The authors identified two amino acid residues (232 and 235) in the lipase hydrophobic substrate binding site as crucial for enantiomer discrimination and enzyme activity. Their results demonstrated the high potential of rational engineering to create biocatalysts suitable for industrial applications.
Chemical synthesis is the common process for obtaining vitamin A derivatives. However, the process presents some issues, such as synthesis steps, which could destroy raw materials (use of acid and alkali), create difficulties in the separation of the final product due to the instability of intermediate products, as well as considerable energy consumption and pollution. Accordingly, a green and environmentally friendly manufacturing method has become the current trend in obtaining vitamin A derivatives. To conveniently prepare the immobilized enzyme for the green synthesis of vitamin A palmitate, T. lanuginosus lipase (TLL) was assembled with apatite derived from calcium phosphate in solution to form TLL@apatite hybrid nanoflowers (hNFs) by mimetic biomineralization. Under the optimized conditions, when vitamin A acetate and palmitic acid were applied as substrates, the yield of the product was up to 90.4% [170].
Recombinant CALB produced by K. phaffii was immobilized and used to synthesize vitamin A palmitate by transesterification of vitamin A acetate and palmitic acid in organic solvent with a conversion ability of 54.3% after 15 cycles [129]. As represented in Figure 5, vitamin A palmitate synthesis could be catalyzed by C. antarctica lipase B. Pregabalin, a GABA (gamma-aminobutyric acid) analogue, is a drug developed for treatment of several central nervous system disorders, involving neuropathic pain, fibromyalgia, anxiety, social phobia and epilepsy. The synthesis of pregabalin involves 2-carboxyethyl-3-cyano-5-methylhexanoic acid ethyl ester, an intermediate substance with chiral characteristic. The (S)-2-carboxyethyl-3-cyano-5-methylhexanoic acid enantiomer is the key chiral intermediate for pregabalin synthesis. Considering the significant increase in the use of pregabalin in recent years, different routes have been explored using chemical and enzymatic approaches [171,172].
Lipozyme TL IM ® and Lipolase ® (Novozymes) are commercially available lipases for the kinetic resolution of (S)-2-Carboxyethyl-3-cyano-5-methylhexanoic acid [172]. In particular, high resolution yields (45%) and enantioselectivity (98% enantiomeric excess, ee) were achieved by using commercial Lipolase, a recombinant T. lanuginosus lipase expressed in A. oryzae [173]. Figure 6 illustrates an optimized route of pregabalin synthesis by enzymatic resolution and decarboxylation steps, considering the work of Martinez et al. [173]. Pregabalin, a GABA (gamma-aminobutyric acid) analogue, is a drug developed for treatment of several central nervous system disorders, involving neuropathic pain, fibromyalgia, anxiety, social phobia and epilepsy. The synthesis of pregabalin involves 2-carboxyethyl-3-cyano-5methylhexanoic acid ethyl ester, an intermediate substance with chiral characteristic. The (S)-2carboxyethyl-3-cyano-5-methylhexanoic acid enantiomer is the key chiral intermediate for pregabalin synthesis. Considering the significant increase in the use of pregabalin in recent years, different routes have been explored using chemical and enzymatic approaches [171,172].
Lipozyme TL IM ® and Lipolase ® (Novozymes) are commercially available lipases for the kinetic resolution of (S)-2-Carboxyethyl-3-cyano-5-methylhexanoic acid [172]. In particular, high resolution yields (45%) and enantioselectivity (98% enantiomeric excess, ee) were achieved by using commercial Lipolase, a recombinant T. lanuginosus lipase expressed in A. oryzae [173]. Figure 6 illustrates an optimized route of pregabalin synthesis by enzymatic resolution and decarboxylation steps, considering the work of Martinez et al. [173]. A mutant L206F/P207F/L259F of T. thermophilus lipase was efficiently immobilized onto epoxy resin D5730 to enable the reuse (using n-heptane/water biphasic system) of the enzyme and to improve the conversion at a high concentration of 2-carboxyethyl3-cyano-5-methylhexanoic acid ethyl ester with 49.7% conversion and 95% p (enantiomeric excess of the product) under the optimized conditions [172].
Diltiazem hydrochloride is a typical calcium channel blocker clinically used as an antianginal and antihypertensive agent [174]. coli often promotes inclusion bodies, which is one of the major problems in industrial recombinant protein production [174,175]. A simple and efficient method for refolding with simultaneous purification of S. marcescens lipase in E. coli in the production of (−)-MPGM has been described with an overall yield of 41.5% and ee of 99% [175]. Figure 7 shows a lipase-catalyzed scheme of the synthetic process of diltiazem. Beta-blockers belong to an important therapeutic class, which is used to treat several cardiovascular conditions [176]. Chirality is a key factor to consider for the efficacy of this class of drugs. Atenolol is a synthetic, beta1-selective (cardioselective) adrenoreceptor blocking agent and it is used in the treatment of hypertension, angina pectoris due to coronary atherosclerosis, as well as acute myocardial infarction angina [177]. It is known that its b1-blocking activity is attributed to its A mutant L206F/P207F/L259F of T. thermophilus lipase was efficiently immobilized onto epoxy resin D5730 to enable the reuse (using n-heptane/water biphasic system) of the enzyme and to improve the conversion at a high concentration of 2-carboxyethyl3-cyano-5-methylhexanoic acid ethyl ester with 49.7% conversion and 95% ee p (enantiomeric excess of the product) under the optimized conditions [172].
Diltiazem hydrochloride is a typical calcium channel blocker clinically used as an antianginal and antihypertensive agent [174]. Trans-3-(4 -methoxyphenyl)glycidic acid methyl ester ((2R, 3S)-MPGM or (−)-MPGM) is a key chiral intermediate for its synthesis. Some approaches involving lipases have been applied in the production of this diltiazem intermediate, such as the application of the S. marcescens lipase, a well-known and important lipase for industrial applications.
Lipase from S. marcescens ECU1010 catalyzed production of (−)-MPGM by the enzymatic resolution of trans-3-(4 -methoxyphenyl)glycidic acid methyl ester ((±)-MPGM) with chitosan in an isopropyl ether water biphasic system (Zhao et al., 2010). Overexpression of S. marcescens lipase in E. coli often promotes inclusion bodies, which is one of the major problems in industrial recombinant protein production [174,175]. A simple and efficient method for refolding with simultaneous purification of S. marcescens lipase in E. coli in the production of (−)-MPGM has been described with an overall yield of 41.5% and ee of 99% [175]. Figure 7 shows a lipase-catalyzed scheme of the synthetic process of diltiazem. A mutant L206F/P207F/L259F of T. thermophilus lipase was efficiently immobilized onto epoxy resin D5730 to enable the reuse (using n-heptane/water biphasic system) of the enzyme and to improve the conversion at a high concentration of 2-carboxyethyl3-cyano-5-methylhexanoic acid ethyl ester with 49.7% conversion and 95% p (enantiomeric excess of the product) under the optimized conditions [172].
Diltiazem hydrochloride is a typical calcium channel blocker clinically used as an antianginal and antihypertensive agent [174]. Trans-3-(4′-methoxyphenyl)glycidic acid methyl ester ((2R, 3S)-MPGM or (−)-MPGM) is a key chiral intermediate for its synthesis. Some approaches involving lipases have been applied in the production of this diltiazem intermediate, such as the application of the S. marcescens lipase, a well-known and important lipase for industrial applications.
Lipase from S. marcescens ECU1010 catalyzed production of (−)-MPGM by the enzymatic resolution of trans-3-(4′-methoxyphenyl)glycidic acid methyl ester ((±)-MPGM) with chitosan in an isopropyl ether water biphasic system (Zhao et al., 2010). Overexpression of S. marcescens lipase in E. coli often promotes inclusion bodies, which is one of the major problems in industrial recombinant protein production [174,175]. A simple and efficient method for refolding with simultaneous purification of S. marcescens lipase in E. coli in the production of (−)-MPGM has been described with an overall yield of 41.5% and ee of 99% [175]. Figure 7 shows a lipase-catalyzed scheme of the synthetic process of diltiazem. Beta-blockers belong to an important therapeutic class, which is used to treat several cardiovascular conditions [176]. Chirality is a key factor to consider for the efficacy of this class of drugs. Atenolol is a synthetic, beta1-selective (cardioselective) adrenoreceptor blocking agent and it is used in the treatment of hypertension, angina pectoris due to coronary atherosclerosis, as well as acute myocardial infarction angina [177]. It is known that its b1-blocking activity is attributed to its Lipase-catalyzed scheme of the synthetic process for diltiazem (adapted from Shibatani et al. [174]).
Beta-blockers belong to an important therapeutic class, which is used to treat several cardiovascular conditions [176]. Chirality is a key factor to consider for the efficacy of this class of drugs. Atenolol is a synthetic, beta1-selective (cardioselective) adrenoreceptor blocking agent and it is used in the treatment of hypertension, angina pectoris due to coronary atherosclerosis, as well as acute myocardial infarction angina [177]. It is known that its b1-blocking activity is attributed to its (S)-enantiomer [178]. In a screening with commercially available lipases, Candida antarctica lipase-A was considered adequate in a new chemoenzymatic route for the (R)-and (S)-atenolol synthesis based on an enzymatic kinetic resolution approach. Compared to the other published methods, the synthesis of the intermediates in an aqueous medium and enzymatic kinetic resolution in an ionic liquid improved overall yield and made the process greener [179].
Lipase B from Candida antarctica (CALB) is one of the most used lipases for pharmaceuticals manufacturing, mainly to obtain key enantioselective intermediates, as well as final enantiomer drug products. There are some characteristics that make this enzyme widely used in pharmaceutical processes, such as good stability, the production of optically pure products, esterification reactions and regioselective modifications of multifunctional substrates. Novozym 435 (Novozymes) is a commercially available immobilized preparation of CALB, being the most used lipase preparation described in the literature. The large-scale production involves the expression of CALB gene in Aspergillus niger and the immobilization of the enzyme on an hydrophobic carrier (acrylic resin) [137,180].
Many pharmaceutical industries and academic research groups have published patents that applied CALB to catalyze reactions in order to obtain pharmaceutical intermediates, as well as "final" active pharmaceutical ingredients [180]. Table 3 shows some examples of granted patents involving enzymatic synthesis of drugs and/or intermediates using CALB.

Conclusions
Lipases are one of the most versatile industrial biocatalysts and the most used enzyme in organic synthesis. For their industrial use, they should be highly stable in specific conditions, active and frequently selective for the substrate. Obviously, most of native lipases are far from being ready for industrial use. In this context, promising lipase candidates from various sources can be optimized through procedures that include strain engineering for enhanced lipase production, lipase engineering with improved properties and lipase immobilization to be reused for long time periods in batches or packed bed reactors. However, these techniques should be applied in combination with bioprocess optimization. The past and recent advances in genetics and computational modelling have incited a revolution in recombinant DNA technologies in addition to complementary techniques, such as molecular dynamics (MD) simulations fields. A great number of new genomes, vectors, genetically modified strains, genetic toolbox, and immobilization and chromatographic matrices are currently available, and their will be an increasing trend in the coming years, accompanying industrial demand. These new technologies have been substantially used in academia and by companies to explore and improve the features of lipases, with the final goal of making them commercial biocatalysts.