Direct Electrochemical Enzyme Electron Transfer on Electrodes Modiﬁed by Self-Assembled Molecular Monolayers

: Self-assembled molecular monolayers (SAMs) have long been recognized as crucial “bridges” between redox enzymes and solid electrode surfaces, on which the enzymes undergo direct electron transfer (DET)—for example, in enzymatic biofuel cells (EBFCs) and biosensors. SAMs possess a wide range of terminal groups that enable productive enzyme adsorption and ﬁne-tuning in favorable orientations on the electrode. The tunneling distance and SAM chain length, and the contacting terminal SAM groups, are the most signiﬁcant controlling factors in DET-type bioelectrocatalysis. In particular, SAM-modiﬁed nanostructured electrode materials have recently been extensively explored to improve the catalytic activity and stability of redox proteins immobilized on electrochemical surfaces. In this report, we present an overview of recent investigations of electrochemical enzyme DET processes on SAMs with a focus on single-crystal and nanoporous gold electrodes. Speciﬁcally, we consider the preparation and characterization methods of SAMs, as well as SAM applications in promoting interfacial electrochemical electron transfer of redox proteins and enzymes. The strategic selection of SAMs to accord with the properties of the core redox protein / enzymes is also highlighted.


Introduction
Self-assembled molecular monolayers (SAMs) are surface monolayers that spontaneously bind to metal surfaces on which, for example, the unique metal-S bonding between metal and thiols offers a versatile pathway to tailor interfacial properties for electrochemical and bioelectrochemical applications [1][2][3][4]. Thiol SAMs on metal surfaces are core targets to provide an understanding of self-organization and interfacial interactions at the molecular level in biological systems [5,6]. Bigelow and associates were the first to demonstrate well-oriented SAMs adsorbed on a platinum wire [7]. However, SAMs did not attract much attention until Nuzzo and associates discovered disulfide monolayers on gold substrates in solution, as a phenomenon different from conventional Langmuir-Blodgett (LB) films [8]. Besides gold and platinum substrates, SAMs can also form on the surfaces of other metals including silver, copper, palladium, and mercury [9][10][11][12][13][14]. Gold is, however, the most extensively investigated because of its chemical inertness, relatively easy handling, and wide potential window, suitable for a range of electrochemical studies [1,15].
In parallel, the nature of the surface Au-S bond has been under intense focus over a number of years [1,2,4] and was recently overviewed [16,17]. In contrast to molecular Au(I)-S(-I) complexes, the "aurophilic" effect arising from collective interactions among surface Au atoms leads to displacement

Spectroscopy
Various spectroscopic techniques are needed to map the complex interactions between SAMs and the metal support [16,17]. X-ray photoelectron spectroscopy (XPS) records the element composition and the chemical state of the SAMs. The high-resolution XPS spectra of S 2p usually show a doublet at the binding energy ranging from 160 to 165 eV, attributed to the formation of the Au-S bond between alkanethiol molecules and the metal support. The functional SAM groups can be identified by Fourier-transform infrared spectroscopy (FTIR), and Raman spectroscopy can be used to detect structural changes in SAMs [68]. Surface plasmon resonance (SPR) spectroscopy is a powerful technique to measure the SAM thickness, showing a change in the tilt angle when the SAM thickness varies. Notably, the adsorption kinetics can be monitored online by SPR coupled with ellipsometry [62].

SAMs and Electrochemical DET of Redox Proteins/Enzymes
SAMs have received considerable early and recent attention as substrates for interfacial bioelectrochemical DET reactions, and a wide range of metalloproteins have been investigated in terms of catalytic mechanism and ET kinetics on various SAM-modified electrode surfaces. The redox active centers of protein/enzymes in DET can be roughly categorized into two major groups: metalbased (iron, copper and molybdenum centers etc.) [20,[69][70][71] and non-metal-based (e.g., flavin adenine dinucleotide (FAD) and pyrrolo-quinoline quinone (PQQ)) centers [44,72,73]. Most, although not all, DET-capable enzymes harbor multiple redox centers, with internal ET relays via heme groups, copper clusters, or Fe-S clusters, which shuttle electrons between the electrode surface and the catalytic cofactors [45,74,75]. In this section, we overview recent studies where SAMs have been used in DET-type electrocatalysis and discuss how to obtain favorable orientations using versatile SAMs, as well as how to tune the interactions between the redox protein/enzyme and the SAM-modified electrodes. Redox proteins/enzymes which can be immobilized in well-defined orientations on SAMmodified supports are illustrated in Figure 2 [4,15,19,32,64,67,70,[76][77][78][79][80][81]. We shall overview and discuss some selected examples from each of these enzyme classes in Sections 3.1-3.3.  (111); in situ STM images of a ω-mercapto-decanoic acid SAM-modified Au(111)electrode surface (b) without protein as a reference, (c) with the two-domain P. stutzeri cyt c4 vertically oriented (sharp roughly circular spots), and (d) horse heart cyt c (sharp roughly circular spots) in 5 mM pH 7.0 phosphate buffer under potential control in constant current mode; scan area, 60 × 60 nm 2 Reproduced with permission from [64]. Copyright 2010, American Chemical Society.

Fructose Dehydrogenase
Although not approaching the degree of detail associated with the simpler ET proteins cyt c and azurin, the molecular mechanistic mapping of several flavin dehydrogenases has reached an impressive level of detail over the last few years, represented by fructose dehydrogenase (FDH), CDH, and GDH in particular. The three enzymes display a common pattern, with a FAD catalytic center where fructose, cellobiose, and glucose, respectively, is oxidized, and an ET relays temporarily populated ET sites through which the liberated electrons are transmitted to the electrode surface. As the first FAD enzyme in our overview, Gluconobacter sp. FDH is a membrane-bound FAD-dependent oxidoreductase with a molecular mass around 140 kDa [25,30]. The protein holds three subunits: subunit I (67 kDa) contains a FAD cofactor, serving as the catalytic center for the two-electron oxidation of D-fructose to keto-D fructose. Subunit II (51 kDa) has three heme groups with the formal potentials of 0.15, 0.06, and −0.01 V vs. Ag/AgCl electrode (sat. KCl), respectively. Only two heme groups with the relatively lower redox potentials are proposed to participate in DET [30]. Subunit III (20 kDa) plays an important role in maintaining the structural integrity of the enzyme complex. A number of recent studies illustrate the employment of FDH for the development of biosensors and biofuel cells with high current density and operational stability [30,81,101,102].
The three-dimensional crystal structure, especially the enzyme surface properties, is essential for rational tuning of the redox enzyme immobilization. The detailed crystallography of FDH is still unclear, but homology models have helped to provide a clearer picture of intramolecular electron transfer (IET) [103]. Kano and associates constructed FDH variants with glutamine instead of the axial methionine ligands (M301, M450, or M578) of heme 1c, heme 2c, and heme 3c, respectively, illustrating that the ET pathway leads from M578 to M450 bypassing M301 [104]. Heme 1c with the highest formal potential of 0.15 V vs. Ag/AgCl electrode was evaluated not to be involved in DET, whereas heme 2c was identified as the ET bridge between FDH and the electrode surface. Heme 3c Figure 3. (a) Schematic illustration of P. stutzeri cyt c 4 (left) and horse heart cyt c (right) on SAM-modified Au (111); in situ STM images of a ω-mercapto-decanoic acid SAM-modified Au(111)-electrode surface (b) without protein as a reference, (c) with the two-domain P. stutzeri cyt c 4 vertically oriented (sharp roughly circular spots), and (d) horse heart cyt c (sharp roughly circular spots) in 5 mM pH 7.0 phosphate buffer under potential control in constant current mode; scan area, 60 × 60 nm 2 Reproduced with permission from [64]. Copyright 2010, American Chemical Society.
Overall, these reports highlight cyt c as a core electron carrier enabling efficient ET between redox enzymes and the electrode surface across suitably chosen SAMs and along with the blue ET protein azurin as a case for characterization in unique detail, right down to the level of the single molecule. Many oxidoreductases furthermore rely on cytochrome domains or subunits as "built-in" ET relays between the catalytically active cofactor and the electrode surfaces and will be discussed in the following sub-sections [100].

Fructose Dehydrogenase
Although not approaching the degree of detail associated with the simpler ET proteins cyt c and azurin, the molecular mechanistic mapping of several flavin dehydrogenases has reached an impressive level of detail over the last few years, represented by fructose dehydrogenase (FDH), CDH, and GDH in particular. The three enzymes display a common pattern, with a FAD catalytic center where fructose, cellobiose, and glucose, respectively, is oxidized, and an ET relays temporarily populated ET sites through which the liberated electrons are transmitted to the electrode surface. As the first FAD enzyme in our overview, Gluconobacter sp. FDH is a membrane-bound FAD-dependent oxidoreductase with a molecular mass around 140 kDa [25,30]. The protein holds three subunits: subunit I (67 kDa) contains a FAD cofactor, serving as the catalytic center for the two-electron oxidation of D-fructose to keto-D fructose. Subunit II (51 kDa) has three heme groups with the formal potentials of 0.15, 0.06, and −0.01 V vs. Ag/AgCl electrode (sat. KCl), respectively. Only two heme groups with the relatively lower redox potentials are proposed to participate in DET [30]. Subunit III (20 kDa) plays an important role in maintaining the structural integrity of the enzyme complex. A number of recent studies illustrate the employment of FDH for the development of biosensors and biofuel cells with high current density and operational stability [30,81,101,102]. The three-dimensional crystal structure, especially the enzyme surface properties, is essential for rational tuning of the redox enzyme immobilization. The detailed crystallography of FDH is still unclear, but homology models have helped to provide a clearer picture of intramolecular electron transfer (IET) [103]. Kano and associates constructed FDH variants with glutamine instead of the axial methionine ligands (M301, M450, or M578) of heme 1c, heme 2c, and heme 3c, respectively, illustrating that the ET pathway leads from M578 to M450 bypassing M301 [104]. Heme 1c with the highest formal potential of 0.15 V vs. Ag/AgCl electrode was evaluated not to be involved in DET, whereas heme 2c was identified as the ET bridge between FDH and the electrode surface. Heme 3c with the lowest formal potential of −0.01 V vs. Ag/AgCl (sat. KCl) was suggested as a bridge between FAD and heme 2c in the IET process ( Figure 4) [30]. In addition, the catalytic current density of FDH was dramatically increased by deleting the amino acid residues on the N-or C-terminus of subunit II [30,105,106]. The deletion not only promotes enzyme loading but also provides more opportunities for favorable orientations on the electrode surface. Some researchers reported that hydrophobic anthracene groups anchored on single-walled carbon nanotubes are favorable for enhanced catalytic activity and stability via the hydrophobic C-terminal region of subunit II [101,107]. These studies suggest that orientation and enzyme loading are crucial for controlling the catalytic activity in DET-type bioelectrocatalysis. with the lowest formal potential of −0.01 V vs. Ag/AgCl (sat. KCl) was suggested as a bridge between FAD and heme 2c in the IET process ( Figure 4) [30]. In addition, the catalytic current density of FDH was dramatically increased by deleting the amino acid residues on the N-or C-terminus of subunit II [30,105,106]. The deletion not only promotes enzyme loading but also provides more opportunities for favorable orientations on the electrode surface. Some researchers reported that hydrophobic anthracene groups anchored on single-walled carbon nanotubes are favorable for enhanced catalytic activity and stability via the hydrophobic C-terminal region of subunit II [101,107]. These studies suggest that orientation and enzyme loading are crucial for controlling the catalytic activity in DETtype bioelectrocatalysis. Favorable FDH SAM immobilization rests on the following consideration: the isoelectric point (IEP) of FDH is 6.59, which means that FDH is overall positively charged in slightly acidic electrolyte [81]. Recent studies to improve the catalytic performance of FDH on various SAM-modified electrodes have been reported [25,41,44,81]. Bollella and associates reported extensive research on the catalytic activity of FDH on highly porous gold (h-PG) electrodes modified with 4-mercaptobenzoic acid (4-MBA), 4-mercaptophenol (4-MPh), and 4-aminothiophenol (4-APh) SAMs. The data showed that high bioelectrocatalytic activity and stability of FDH was only observed with -OH terminated SAMs [81], suggesting optimized enzyme orientation on this particular SAM. Negatively charged SAMs may help to favor FDH coverage due to preferred electrostatic interaction, but this is not necessarily the most favorable FDH orientation for DET. Murata and associates reached similar conclusions for FDH immobilized on 2-mercaptoethanol (MET) SAM-modified AuNPs [41]. Considering the importance of the gold nanostructure for the ET rate, the AuNP size is furthermore a crucial parameter. Kizling and associates synthesized 1.0 to 3.5 nm AuNP clusters functionalized with 1,6-hexanedithiol and 1-butanethiol for investigating the ET mechanism. A channel of "mediated" catalysis, i.e., electron "hopping", was observed for the smallest AuNP clusters around 1 nm with the half-wave potential close to the first oxidation potential of the AuNP at the edge of the HOMO-LUMO gap [44]. Such a mode accords with theoretical notions recently reported [92]. Siepenkoetter and associates reported the catalytic performance of covalently bonded FDH on NPG electrodes with varying pore sizes [25]. A large number of findings thus show that FDH has high affinity for well-defined SAM-modified electrodes with the polar but electrostatically neutral hydroxyl terminal group as the most efficient, indicating the importance of hydrophilicity of the electrode surface towards the mixed surface charge distribution of the FDH target enzyme. However, details of the underlying mechanism remain unknown.3.1.

Cellobiose Dehydrogenase
The second FAD enzyme, cellobiose dehydrogenase (CDH), is a versatile oxidoreductase for direct bioelectrocatalysis. CDH contains a catalytic dehydrogenase domain (DH) harboring a FAD as the redox center and an ET cytochrome b (CYT) domain to shuttle electrons from the FAD to the electrode surface [23,45,108]. The two domains are separated in the crystalline structure, but an Favorable FDH SAM immobilization rests on the following consideration: the isoelectric point (IEP) of FDH is 6.59, which means that FDH is overall positively charged in slightly acidic electrolyte [81]. Recent studies to improve the catalytic performance of FDH on various SAM-modified electrodes have been reported [25,41,44,81]. Bollella and associates reported extensive research on the catalytic activity of FDH on highly porous gold (h-PG) electrodes modified with 4-mercaptobenzoic acid (4-MBA), 4-mercaptophenol (4-MPh), and 4-aminothiophenol (4-APh) SAMs. The data showed that high bioelectrocatalytic activity and stability of FDH was only observed with -OH terminated SAMs [81], suggesting optimized enzyme orientation on this particular SAM. Negatively charged SAMs may help to favor FDH coverage due to preferred electrostatic interaction, but this is not necessarily the most favorable FDH orientation for DET. Murata and associates reached similar conclusions for FDH immobilized on 2-mercaptoethanol (MET) SAM-modified AuNPs [41]. Considering the importance of the gold nanostructure for the ET rate, the AuNP size is furthermore a crucial parameter. Kizling and associates synthesized 1.0 to 3.5 nm AuNP clusters functionalized with 1,6-hexanedithiol and 1-butanethiol for investigating the ET mechanism. A channel of "mediated" catalysis, i.e., electron "hopping", was observed for the smallest AuNP clusters around 1 nm with the half-wave potential close to the first oxidation potential of the AuNP at the edge of the HOMO-LUMO gap [44]. Such a mode accords with theoretical notions recently reported [92]. Siepenkoetter and associates reported the catalytic performance of covalently bonded FDH on NPG electrodes with varying pore sizes [25]. A large number of findings thus show that FDH has high affinity for well-defined SAM-modified electrodes with the polar but electrostatically neutral hydroxyl terminal group as the most efficient, indicating the importance of hydrophilicity of the electrode surface towards the mixed surface charge distribution of the FDH target enzyme. However, details of the underlying mechanism remain unknown. 3

.1.3. Cellobiose Dehydrogenase
The second FAD enzyme, cellobiose dehydrogenase (CDH), is a versatile oxidoreductase for direct bioelectrocatalysis. CDH contains a catalytic dehydrogenase domain (DH) harboring a FAD as the redox center and an ET cytochrome b (CYT) domain to shuttle electrons from the FAD to the electrode surface [23,45,108]. The two domains are separated in the crystalline structure, but an integrated IET pathway can be opened through a flexible and hydrophilic amino acid linker between the catalytic and CYT domains [80]. This motif is encountered also for the molybdenum sulfite oxidases, cf. Section 3.1.4. CDH is extracted from the phyla Basidiomycota and Ascomycota, divided into class I, class II, and class III, respectively. Class III CDH from Ascomycota remains uncertain compared to class I and class II CDH [23].
The natural substrates of CDH mainly include cellulose, lactose, and glucose [108]. Class I CDH with short amino acid sequences shows direct, strongly pH-dependent catalytic activity only in solutions with pH below 5.5 [23]. Harreither and associates reported extensive studies on class II CDH from Chaetomium attrobrunneum (CaCDH), Corynascus thermophiles (CtCDH), Dichomera saubinetii (DsCDH), Hypoxylon haematostroma (HhCDH), Neurospora crassa (NcCDH), and Stachybotrys bisbyi (SbCDH) [109]. pH-dependent catalytic activity was observed for neutral and slightly alkaline electrolytes with cyt c and 2,6-dichloroindophenol (DCIP) as electron acceptors. The different electrostatic environment in class I and II CDH reveals different optimal IET processes, with optimum IET in acidic electrolyte for class I CDH and neutral or slightly alkaline electrolyte for class II CDH [110]. Schulz and associates demonstrated turnover and non-turnover DET performance of CDH on polycrystalline gold modified with MUO or 6-MHO [23]. A clear catalytic current between the FAD and the electrode surface was reported. The midpoint potential of bound FAD cofactor was −163 mV vs. SCE at pH 3.0, approximately 130 mV less than that of the heme b relay, leading to a much lower onset potential for lactose oxidation. The authors concluded that the tunneling distance between the FAD cofactor and the electrode was around 12-15 Å, thereby allowing direct electrochemical communication.
The authors also demonstrated that only class I CDH from Trametes villosa (TvCDH) and Phanerochaete sordida (PsCDH) displayed DET activity, whereas no DET signal was observed for class II CDH from reconstructed Myriococcum thermophilum (recMtCDH) and reconstructed Corynascus thermophilus (recCtCDH) at low pH.
The IEP of CDH (DH domain~5, CYT domain~3) gives a negatively charged surface around the ET path exit, indicating that positively charged SAMs are suitable for favorable DET [110]. Lamberg and associates anchored Humicola insolens CDH on various SAMs with different terminal groups for investigating the effects of charge and hydrophobicity [111] and found that hydrophilic SAMs were favorable for high catalytic activities, with lower enzyme orientation variations than for hydrophobic SAMs. Tavahodi and associates reported a DET-type lactose biosensor of PsCDH on polyethyleneimine (PEI)-coated AuNP electrodes (PEI@AuNP) [112]. PEI with positively charged amino groups not only optimized the orientation of PsCDH on the electrode to increase the IET rate but also gave high enzyme stability and sensitivity. Bollella and associates reported a lactose biosensor based on DET of CtCDH on gold electrodes [113]. The authors demonstrated that BPDT SAMs with two thiol groups can be used to anchor metal NPs on a gold electrode surface by covalent bonding, showing the best ET rate on AuNPs/BPDT/Au electrode. A mediator-free HiCDH/MvBOD BFC using a positively charged MHP SAM for immobilization on AuNP-modified gold electrodes was also reported [114]. The half-life time, i.e., the time duration over which the activity decreased to half the initial value, was 13 and 44 h with 5 mM glucose and 10 mM lactose in neutral buffer solution, respectively. Hirotoshi and associates found an efficient ET process of Phanerochaete chrysosporium (PcCDH) on a mixed 11-AUT/MUO SAM-modified AuNP electrode [46]. Al-Lolage and coworkers addressed the catalytic performance and stability of MtCDH by introducing cysteine mutants (E522 and T701) on the protein surface ( Figure 5) [45]. The cysteine mutant with a surface thiol group made it possible to form stable thioether bonds with maleimide groups via click-chemistry, thereby controlling the orientations of the MtCDH mutant on the electrode surface and revealing efficient electrochemical glucose oxidation. Another example reported by Meneghello and associates clearly indicated that the DET-type bioelectrocatalysis of CDH is highly sensitive to the cysteine residues introduced at particular positions [115]. Experimental results also showed that divalent cations (i.e., Ca 2+ and Mg 2+ ) affect the IET kinetics [23,115].

FAD-Dependent Glucose Dehydrogenase
Our final FAD enzyme target, glucose dehydrogenase (FAD-GDH), is one of the most widely known dehydrogenases with a tightly bound cofactor, making it different from NAD + -dependent GDH. FAD-GDH has been extensively studied as an emerging alternative to glucose oxidase (GOx) due to its favorable DET capability, insensitivity to dioxygen, and the fact that no hydrogen peroxide is generated [116][117][118][119]. The structure of GDH is analogous to that of FDH, comprising three subunits: an FAD-dependent catalytic subunit, an ET subunit with three heme groups, and a small "hitchhiker" protein used for the flexibility of the catalytic subunit into the periplasm [116,120]. The catalytic subunit harbors a 3Fe-4S cluster close to the ET subunit protein surface, allowing efficient DET on the electrode without the need for mediators. Lee and associates demonstrated controlled DET of bacterial FAD-GDH from Burkholderia cepacia on three different SAM-modified electrodes, on which catalytic current density decreases with increasing SAM chain length [118]. A glucose biosensor based on FAD-GDH was reported recently by introducing a gold-binding peptide (GBP) for enzyme immobilization on a screen-printed electrode (SPE) [24]. GBP composed of 12 amino acids exhibiting a strong binding affinity to the gold electrode surface was fused to the enzyme terminus, thereby determining the enzyme orientation on the electrode. Around 10 times higher catalytic response toward 100 mM glucose was observed with FAD-GDH-GBP/Au compared with normal GDH/Au.
A DET-type glucose biosensor could also be fabricated coupled with electrochemical impedance spectroscopy (EIS) [120]. Three variable-length thiols, dithiobis(succinimidyl hexanoate) (DSH), dithiobis(succinimidyl octanoate) (DSO), and dithiobis(succinimidyl undecanoate) (DSU), were employed to modify the electrode surface. Charge transfer resistance (Rct) was a key parameter reflecting the DET efficiency, with the lowest resistance when FAD-GDH was immobilized on DSH SAMs because of the shortest tunneling distance. In addition, the steady-state catalytic current density of FAD-GDH was dramatically increased on AuNPs assembled on the gold electrode [73,121]. Ratautas and associates reported high glucose oxidation activities of FAD-GDH extracted from Ewingella Americana without mediator [73,121]. They demonstrated that FAD-GDH immobilized on 4-ATP-modified AuNPs displayed higher catalytic activity and lower overpotential than on 4-MBAmodified electrodes. Notably, 4-ATP can be oxidized in neutral media and further converted to 4-

FAD-Dependent Glucose Dehydrogenase
Our final FAD enzyme target, glucose dehydrogenase (FAD-GDH), is one of the most widely known dehydrogenases with a tightly bound cofactor, making it different from NAD + -dependent GDH. FAD-GDH has been extensively studied as an emerging alternative to glucose oxidase (GOx) due to its favorable DET capability, insensitivity to dioxygen, and the fact that no hydrogen peroxide is generated [116][117][118][119]. The structure of GDH is analogous to that of FDH, comprising three subunits: an FAD-dependent catalytic subunit, an ET subunit with three heme groups, and a small "hitch-hiker" protein used for the flexibility of the catalytic subunit into the periplasm [116,120]. The catalytic subunit harbors a 3Fe-4S cluster close to the ET subunit protein surface, allowing efficient DET on the electrode without the need for mediators. Lee and associates demonstrated controlled DET of bacterial FAD-GDH from Burkholderia cepacia on three different SAM-modified electrodes, on which catalytic current density decreases with increasing SAM chain length [118]. A glucose biosensor based on FAD-GDH was reported recently by introducing a gold-binding peptide (GBP) for enzyme immobilization on a screen-printed electrode (SPE) [24]. GBP composed of 12 amino acids exhibiting a strong binding affinity to the gold electrode surface was fused to the enzyme terminus, thereby determining the enzyme orientation on the electrode. Around 10 times higher catalytic response toward 100 mM glucose was observed with FAD-GDH-GBP/Au compared with normal GDH/Au.
A DET-type glucose biosensor could also be fabricated coupled with electrochemical impedance spectroscopy (EIS) [120]. Three variable-length thiols, dithiobis(succinimidyl hexanoate) (DSH), dithiobis(succinimidyl octanoate) (DSO), and dithiobis(succinimidyl undecanoate) (DSU), were employed to modify the electrode surface. Charge transfer resistance (R ct ) was a key parameter reflecting the DET efficiency, with the lowest resistance when FAD-GDH was immobilized on DSH SAMs because of the shortest tunneling distance. In addition, the steady-state catalytic current density of FAD-GDH was dramatically increased on AuNPs assembled on the gold electrode [73,121]. Ratautas and associates reported high glucose oxidation activities of FAD-GDH extracted from Ewingella Americana without mediator [73,121]. They demonstrated that FAD-GDH immobilized on 4-ATP-modified AuNPs displayed higher catalytic activity and lower overpotential than on 4-MBA-modified electrodes. Notably, 4-ATP can be oxidized in neutral media and further converted to 4-mercapto-N-phenylquinone monoimine (MPQM), the quinone groups of which could bind covalently with primary amino groups of the enzyme, thereby enhancing the ET rate. The catalytic response was found to be positively correlated to the ratio of 4-ATP/4-MBA for electrodes modified by mixed thiol SAMs [121]. PQQ-dependent GDH is still another promising GOx candidate, widely utilized for glucose biosensors and biofuel cells [35,95,96]. Kim and coworkers prepared a glucose biosensor by immobilizing positively charged PQQ-GDH on MUA SAM-modified gold electrodes [122]. In this case, PQQ-GDH exhibited higher current density and detection sensitivity via electrostatic adsorption than via covalent bonding, due to less enzyme inactivation. Covalent bonding could thus enhance the interactions between enzyme and electrode surface, but the surface enzyme characteristics are the more critical factors that determine the electrochemical behavior.

Sulfite Oxidase
Sulfite oxidase (SOx) is another heme-containing redox enzyme which has been studied for a long time. Two kinds of SOx are mainly employed in DET-type bioelectrocatalysis: chicken liver, cSOx [123], and human, hSOx [124]. SOx acts by a principle resembling that of the FAD enzymes, with a catalytic center and an electronic relay, but also with some important differences. As a metalloprotein, the catalytic domain is composed of a pyranopterin molybdenum (Mo) cofactor effecting two-electron oxidation of sulfite to sulfate and connected to a N-terminal cytochrome b 5 (cyt b 5 ) domain by a flexible linker [123,125]. Although the crystallographic structure of cSOx shows that the distance between the catalytic Mo domain and the cyt b 5 domain is more than 32 Å, rapid internal ET in DET-type bioelectrocatalysis is still observed [126], effected by a conformational change on the electrode surface via the flexible tether. This "on-off" switch enables cyt b 5 to be either adjacent or remote from the Mo cofactor [70,127] in a gated ET mode, which is quite different from the rigidly bound ET relays in the FDH and GDH enzymes, but similar to the CDH operational mode.
The electrostatic surface charge distributions around both domains are complex and highlight the importance of subtle tuning of the electrode surfaces [124]. A positively charged SAM surface is favorable for immobilization of hSOx via the Mo domain, directing the smaller heme domain towards the electrode surface. As noted, a similar pattern with a flexible linker connecting the catalytic and ET domain applies to CDH [80]; cf. Section 3.1.3. Sezer and associates investigated the catalytic activity of hSOx on a mixed SAM-modified silver electrode, showing a significantly increased SERR signal when increasing the ionic strength. High ionic strength is favored to shorten the distance between the Mo cofactor and the heme domain, thereby facilitating both intramolecular and interfacial ET. Wollenberger and associates reported other studies of the electrochemical behavior of hSOx [127][128][129][130]. In situ scanning tunneling microscopy and spectroscopy to single-molecule resolution has, finally been reported quite recently and disclosed intriguing patterns of tunneling via the Mo-and heme group redox centers [130]. AuNPs with a diameter less than 10 nm were covalently bonded on the MUA/MUO SAM-modified gold electrode, giving a significant enhancement of the interfacial ET rate of hSOx [129]. The interfacial ET rate could be further increased by using SAMs with 3,3 -dithiodipropionic acid di(N-hydroxysuccinimide ester) (DTSP) thinner than MUA/MUO SAMs [128]. They also reported that the introduction of BaSO 4 nanoparticles played a significant role in the DET-type bioelectrocatalysis of hSOx. The electrochemical communication between the active sites of hSOx and the electrode surface could be further enhanced by using a positively charged biopolymer. Kalimuthu and coworkers thus reported direct catalytic activity of hSOx on a chitosan-covered gold electrode [131]. Both non-catalytic redox signals corresponding to the heme group and catalytic signals in the presence of 4 mM sulfite, on chitosan-covered MPA-, MSA-, and 4-MBA-modified electrodes were observed. However, there were no catalytic signals on MUA-based electrodes despite an observed non-turnover feature, highlighting the importance of rational SAM selection.

Azurin
Pseudomonas aeruginosa azurin is a simple blue copper protein that undergoes single-ET between the Type 1 copper atom and the electrode, now developed as a single-molecule "core" target [6,[132][133][134][135][136], as for cyt c characterized and mapped in unique detail. A hydrophobic patch and the disulfide group at opposite ends of the azurin molecule are both critical for well-defined orientations on the electrode. Chi, Ulstrup, Zhang and associates conducted extensive investigations of the electrochemical behavior of azurin [39,43,51,137]. In situ STM disclosed arrays of well-organized self-assembled azurin monolayers on single-crystal Au(111)-electrodes mapped to single-molecule resolution [51]. Hydrophobic alkanethiol monolayers were employed for gentle immobilization by hydrophobic interactions with the hydrophobic patch of azurin [43]. Notably, exponential decay of the ET rate constant with increasing chain length was observed for chain lengths longer than six carbon atoms, reflecting a dual mechanism, with tunneling dominating for the longer chains [39]. The ET kinetics and redox mechanism of azurin have been analyzed theoretically at different levels, as discussed in detail elsewhere [6,138]. Inserting 3-4 nm coated AuNPs as for horse heart cyt c [83] results in a 20-fold enhancement of k app (220 ± 16 s −1 ) for azurin compared with the AuNP-free system (10.2 ± 0.4 s −1 ) [51]. Armstrong and associates compared the ET kinetics of azurin on the electrode modified with a synthetic {3,5-diethoxy-4-[(E)-2-(4-ethylphenyl)vinyl]-phenyl} methanethiol or commercial-CH 3 terminal alkanethiol [139]. The front hydrophobic ethyl group of SAMs served as the protein-binding bridge on the surface, yielding a very fast ET rate with a k app over 1600 s −1 .

Copper Nitrite Reductase
A common feature of the blue multi-copper oxidases is a blue T 1 center for the electron inlet and a T II or combined T II /T III catalytic center for the electron "outlet" in the catalytic process (nitrite, or, dioxygen reduction). Such an electrochemical mode of action is only feasible via an efficient (short) intramolecular ET channel. This feature has been mapped in considerable detail for three blue copper enzyme classes, the copper nitrite reductases, bilirubin oxidases, and the laccases. The trimeric blue copper nitrite reductase (CuNiR) is crucial in the global nitrogen cycle, catalyzing the single-electron reduction of nitrite to nitrogen monoxide [140]. CuNiR effects direct bioelectrocatalysis, including an ET relay (Cu I ) and a catalytic site (Cu II ) in each monomer. Ulstrup and associates reported DET-based electrocatalysis of CuNiR from Achromobacter xylosoxidans (AxCuNiR) on a cysteamine SAM-modified Au(111) electrode [141]. In situ STM displayed single AxCuNiR molecules but, intriguingly, only in the presence of nitrite substrate. Further, the combination of varying alkanethiols with charged, neutral, hydrophilic, and hydrophobic properties showed that mixed hydrophilic/hydrophobic SAMs were the most favorable for facile AxCuNiR electrocatalysis [142]. In situ AFM is also reported and disclosed AxCuNiR conformational changes during catalytic reaction [67], with the apparent height of AxCuNiR rising from 4.5 nm in the resting state to 5.5 nm in the nitrite reduction state in the presence of nitrite.

Bilirubin Oxidase
Bilirubin oxidase (BOD) is another well-known blue multicopper oxidase class containing four copper centers (T 1 , T 2 /T 3 ), catalyzing the dioxygen reduction reaction (ORR) into water in four-electron direct bioelectrocatalysis [75,96]. The blue T 1 center has a copper atom located closed to the protein surface for accepting electrons from the natural electron donor (bilirubin) or an electrode. The external electrons are relayed via a short ligand-bound intramolecular peptide bridge to the T 2 /T 3 center, where ORR takes place. Target BODs are mainly from Myrothecium verrucaria (MvBOD) [26,29,75,[143][144][145], Trachyderma tsunodae (TtBOD) [146], Bacillus pumilus (BpBOD) [147], and Magnaporthe oryzae (MoBOD) [148]. Single-crystal gold electrodes modified with -NH 2 , -COOH, -OH, and -CH 3 terminated SAMs have been studied and show that negatively charged SAMs are favorable for proper MvBOD orientations due to the highly positively charged region close to the T 1 center [42]. E • values of T 1 (0.69 V vs. NHE) and T 2 /T 3 (0.39 V vs. NHE) in TtBOD have been observed under aerobic conditions [146], but E • of the T 2 /T 3 centers drops to 0.36 V vs. NHE in the resting state of the enzyme, indicative of an IET pathway from T 1 to T 2 /T 3 triggered by the ORR process.
Characterization of enzyme loading and conformation on the electrode is crucial [149]. Lojou and associates studied the adsorption of MvBOD on both positively (NH 3 + ) and negatively (COO -) charged SAM-modified electrodes with no significant difference in enzyme loading as disclosed by surface plasmon resonance (SPR) spectroscopy [26]. DET and MET processes were found to dominate on the COOand NH 3 + surfaces, respectively, consistent with the positively charged surroundings of the T 1 Cu [26]. Polarization-modulated infrared reflection absorption spectroscopy (PMIRRAS) showed strong electrostatic interactions between the negatively charged SAMs and the positively charged MvBOD surface near the T 1 Cu [26]. PMIRRAS and SPR ellipsometry were jointly used to demonstrate the effect of electrostatic interactions on the enzyme adsorption, catalytic performance, and stability of MvBOD at four different pH values ( Figure 6) [75]. The dipole moment of the enzyme is distinct at different pH levels, showing a direction towards the T 1 center in neutral or slightly acidic electrolytes but significantly shifted in the strongly acid electrolyte (Figure 6a). This accords with the different charge distributions at the T 1 Cu center. PMIRRAS spectra showed the amide I (ca. 1680 cm −1 ) and amide II (ca. 1550 cm −1 ) peaks, related to vibrational C=O and N-H modes in MvBOD, respectively, see Figure 6b. The wavelengths of the two peaks remained unchanged upon enzyme immobilization, which suggests that the secondary structure of the MvBOD is retained. In addition, amide I/amide II ratios were similar at different pH levels, further indicating that the orientations of MvBOD were independent of pH.
the T1 Cu [26]. Polarization-modulated infrared reflection absorption spectroscopy (PMIRRAS) showed strong electrostatic interactions between the negatively charged SAMs and the positively charged MvBOD surface near the T1 Cu [26]. PMIRRAS and SPR ellipsometry were jointly used to demonstrate the effect of electrostatic interactions on the enzyme adsorption, catalytic performance, and stability of MvBOD at four different pH values ( Figure 6) [75]. The dipole moment of the enzyme is distinct at different pH levels, showing a direction towards the T1 center in neutral or slightly acidic electrolytes but significantly shifted in the strongly acid electrolyte (Figure 6a). This accords with the different charge distributions at the T1 Cu center. PMIRRAS spectra showed the amide I (ca. 1680 cm −1 ) and amide II (ca. 1550 cm −1 ) peaks, related to vibrational C=O and N-H modes in MvBOD, respectively, see Figure 6b. The wavelengths of the two peaks remained unchanged upon enzyme immobilization, which suggests that the secondary structure of the MvBOD is retained. In addition, amide I/amide II ratios were similar at different pH levels, further indicating that the orientations of MvBOD were independent of pH. Enzyme loading, critical to catalytic activity, declines as pH increases (Figure 6c [75]). The authors demonstrated that MvBOD on a negatively charged 6-MHA SAM-modified electrode does not form a saturated monolayer at pH 7.5 but possibly more than a single monolayer at pH 3.6. The electrostatic interactions between enzyme and SAMs thus not only strongly affect the enzyme adsorption, the dipole moment of the enzyme, and the charge around the T1 center, but they also determine the enzyme orientation and catalytic rate (Figure 6d). Gholami and associates immobilized MvBOD on a gold microfilm by electropolymerization of TCA [150]. Molecular dynamics simulation has provided a more comprehensive understanding of MvBOD in DET-type bioelectrocatalysis [151]. In particular, MvBOD showed various orientations reflecting wide charge distributions, representing a "back-on" and "lying-on" state on positively and negatively charged electrodes, respectively.  Enzyme loading, critical to catalytic activity, declines as pH increases (Figure 6c [75]). The authors demonstrated that MvBOD on a negatively charged 6-MHA SAM-modified electrode does not form a saturated monolayer at pH 7.5 but possibly more than a single monolayer at pH 3.6. The electrostatic interactions between enzyme and SAMs thus not only strongly affect the enzyme adsorption, the dipole moment of the enzyme, and the charge around the T 1 center, but they also determine the enzyme orientation and catalytic rate (Figure 6d). Gholami and associates immobilized MvBOD on a gold microfilm by electropolymerization of TCA [150]. Molecular dynamics simulation has provided a more comprehensive understanding of MvBOD in DET-type bioelectrocatalysis [151]. In particular, MvBOD showed various orientations reflecting wide charge distributions, representing a "back-on" and "lying-on" state on positively and negatively charged electrodes, respectively.
Nanostructured gold surfaces are expected to improve the DET current density of BOD [29]. However, the apparent and real current densities normalized to the geometric and real gold surface area, respectively, should be distinguished. In comparison to FDH on 1 nm AuNPs [44], this is particularly important when the gold nanostructures are of larger size than BOD. Pankratov and associates investigated the size effect of sub-monolayer AuNPs (diameter: 20, 40, 60, and 80 nm, significantly larger than MvBOD) on the bioelectrocatalytic performance of MvBOD [152]. Although the apparent ORR current density increased with increasing size from 20 to 80 nm (proportionally to the real surface area), similar values (15 ± 3 uA cm −2 ) for the real current density were obtained. This can be explained by the fact that the ET rate constant is independent of the AuNP size in this case, with similar values of 10.3 ± 0.5 s −1 and 10.7 ± 0.3 s −1 for the MvBOD-based bioelectrode with and without AuNPs, respectively. Siepenkoetter and associates immobilized MvBOD on NPG using diazonium grafting coupled with an MPA SAM [28]. NPG electrodes with average pore sizes between 9 and 62 nm were finally investigated. The maximum apparent ORR current density was achieved with 10 and 25 nm pores, slightly larger than the enzyme and likely due to a compromise between the real gold surface area and enzyme loading, but similar real current densities were registered for these pore sizes.

Laccase
The laccases (Lac) constitute a third important member class of the multicopper oxidases, containing T 1 and T 2 /T 3 centers [27,153,154]. Similar to BOD, T 1 and T 2 /T 3 Cu centers act as electron acceptance and ORR centers, respectively. E • of the T 1 center from tree Lac is lower than those from fungal Lac, varying in the range 300 to 800 mV vs. NHE [155]. Bioelectrocatalysis of fungal Lac has been extensively investigated [156]. SAMs with specific functional groups favor productive orientations of Lac at the electrode surface. Thorum and associates reported that the overpotential of ORR of Lac from Trametes versicolor (TvLac) could be decreased by employing an anthracene-2-methanethiol SAM on gold. The aromatic anthracene presumably penetrates into the hydrophobic pocket close to the T 1 center, facilitating DET [157]. Climent and associates investigated the DET-type catalytic activity of three different Lacs (Coprinus cinereus (CcLac), Myceliophthora thermophila (MtLac), and Streptomyces coelicolor (ScLac)) [38]. In situ STM enabled single-molecule understanding of enzyme-electrode electronic interactions and the IET process on well-defined Au(111) surfaces with various SAMs. MPA SAMs with the carboxyl terminal group was best for CcLac, while alkyl and amino SAMs were most suitable for ScLac. No catalytic signal was found for MtLac on any SAMs. As for AxCuNiR, single-molecule in situ STM contrasts were observed only in the presence of enzyme substrate, nitrite, and dioxygen, respectively. Traunsteiner and coworkers exploited DET-type bioelectrocatalysis of TvLac using diluted MPA SAMs and a linker molecule of thiolated veratric acid (tVA) that could approach the T 1 site [158]. Optimal catalytic activity was shown when the tVA and MPA SAMs were mixed uniformly, whereas the catalytic activity decreased dramatically due to the aggregation of tVA. Molecular dynamics simulations also showed that the positively charged SAMs were more favorable for the DET of TvLac, with a narrow orientation distribution.
A recent study evaluated Thermus thermophilus (TtLac), exhibiting a methionine rich domain. Figure 7 shows the detailed three-dimensional structure and electrostatic charge distributions of the surface amino acid residues of TtLac [69]. The T 1 center, used for transferring electrons to an external electrode, showed a negatively charged zone. Gold electrodes modified with negatively (-COO − ), uncharged (-OH), and positively charged groups (-NH 3 + ) were adopted, with the highest catalytic current on the positively charged electrode and with no or only weak catalytic signals on the negatively charged and uncharged electrodes, respectively [69]. Nanostructured materials have been developed recently to optimize the Lac orientation. NPG modified with 4-ATP SAM could increase the catalytic performance and stability at high temperatures [159]. Cristina and associates reported that AuNPs (particle size: 5 nm, comparable to the size of the enzyme) served as electronic bridges, thus promoting DET with a heterogeneous ET rate constant over 400 s −1 [54]. Nanostructured electrodes consisting of low-density graphite (LDG) and gold nanorods (AuNRs, average length: 31 ± 6 nm, width: 5 ± 1 nm) have, finally, been prepared to orient Lac for improved ORR [27].
Catalysts 2020, 10, x FOR PEER REVIEW 15 of 26 the catalytic performance and stability at high temperatures [159]. Cristina and associates reported that AuNPs (particle size: 5 nm, comparable to the size of the enzyme) served as electronic bridges, thus promoting DET with a heterogeneous ET rate constant over 400 s −1 [54]. Nanostructured electrodes consisting of low-density graphite (LDG) and gold nanorods (AuNRs, average length: 31 ± 6 nm, width: 5 ± 1 nm) have, finally, been prepared to orient Lac for improved ORR [27].

[FeS]-Cluster Hydrogenases
Hydrogenases are a class of [FeS] cluster-based metalloproteins which reversibly catalyze the two-electron reactions of dihydrogen oxidation and evolution [71,74]. The commonly reported membrane-bound hydrogenases can be classified based on their intrinsic catalytic cofactors ([FeFe], [NiFe], and [NiFeSe]), where hydrogen conversion is combined with [FeS] electron relay to accomplish the entire ET process. It has been reported that the smallest [FeFe]-hydrogenase CrHydA1 isolated from Chlamydomonas reinhardtii exhibits dioxygen insensitivity and shows high catalytic activity in dihydrogen evolution [160]. The conditions of immobilized [FeFe]-hydrogenase CrHydA1 on a SAM-modified gold electrode were characterized by in situ surface-enhanced infrared absorption spectroscopy (SEIRAS) and SPR spectroscopy [160]. Madden and associates investigated the catalytic activity of [FeFe]-hydrogenase CaHydA from Clostridium acetobutylicum immobilized on negatively charged MHA-modified Au(111) electrodes [74]. Electrochemical STM showed that the apparent height of [FeFe]-hydrogenase CaHydA continuously increased when the potential of the STM substrate was shifted from −0.4 to −0.6 V (vs. Ag/AgCl electrode), in which the hydrogen evolution response was observed by CV. Notably, the catalysis of hydrogenase is easily quenched by carbon monoxide and cyanide binding to the Fe active sites.

[FeS]-Cluster Hydrogenases
Hydrogenases are a class of [FeS] cluster-based metalloproteins which reversibly catalyze the two-electron reactions of dihydrogen oxidation and evolution [71,74]. The commonly reported membrane-bound hydrogenases can be classified based on their intrinsic catalytic cofactors ([FeFe], [NiFe], and [NiFeSe]), where hydrogen conversion is combined with [FeS] electron relay to accomplish the entire ET process. It has been reported that the smallest [FeFe]-hydrogenase CrHydA1 isolated from Chlamydomonas reinhardtii exhibits dioxygen insensitivity and shows high catalytic activity in dihydrogen evolution [160]. The conditions of immobilized [FeFe]-hydrogenase CrHydA1 on a SAM-modified gold electrode were characterized by in situ surface-enhanced infrared absorption spectroscopy (SEIRAS) and SPR spectroscopy [160]. Madden and associates investigated the catalytic activity of [FeFe]-hydrogenase CaHydA from Clostridium acetobutylicum immobilized on negatively charged MHA-modified Au(111) electrodes [74]. Electrochemical STM showed that the apparent height of [FeFe]-hydrogenase CaHydA continuously increased when the potential of the STM substrate was shifted from −0.4 to −0.6 V (vs. Ag/AgCl electrode), in which the hydrogen evolution response was observed by CV. Notably, the catalysis of hydrogenase is easily quenched by carbon monoxide and cyanide binding to the Fe active sites.
Membrane-bound [NiFe]-hydrogenase and [NiFeSe]-hydrogenase have attracted considerable attention due to their resistance to dioxygen, carbon monoxide, as well as to high temperature [161]. Armstrong and associates reported a number of studies into the oxygen tolerance of [NiFe]-hydrogenase [162,163]. Typically, the catalytic sites of well-known hydrogenases are quenched by dissolved oxygen gas due to the high oxygen sensitivity of the internal peptide chains. Modification of Fe-S clusters by changing two oxygen-sensitive cysteine residues into glycines showed significant improvements in the long-term dihydrogen oxidation performance [162]. The authors demonstrated that the mechanism of oxygen tolerance mainly relates to removal of oxide species rather than preventing oxygen access into the protein. Lojou and associates demonstrated that carboxyl-terminated SAMs were favorable for optimizing the orientations of [NiFe]-hydrogenase from Aquifex aeolicus for efficient DET-type and MET-type bioelectrocatalysis of hydrogen oxidation, whereas hydrophobic SAMs only resulted in a MET process with the need for methylene blue mediator [71]. The electrochemical behavior of [NiFe]-hydrogenase from Allochromatium vinosum, DesulfoVibrio Vulgaris Miyazaki F (DvMF), and Ralstonia eutropha H16 has also been reported [164][165][166].
Immobilization of membrane-bound hydrogenase on the SAM-modified electrode surface appears promising, but the ET progress of membrane-bound hydrogenase is challenging due to the complex enzyme structure compared with soluble redox enzymes. Gutiérrez-Sánchez and coworkers demonstrated that the introduction of a phospholipidic bilayer on positively charged 4-ATP modified Au electrode surfaces effectively controls the orientation of membrane-bound [NiFeSe]-hydrogenase for hydrogen oxidation (Figure 8) [167]. The hydrophobic lipid tail of hydrogenase can be embedded into the phospholipidic bilayer, thereby reducing the orientation distribution and promoting the ET process. All these results suggest that SAM-modified electrodes are paramount to provide a versatile platform for understanding how to tune the right enzyme orientation for DET.  [164][165][166]. Immobilization of membrane-bound hydrogenase on the SAM-modified electrode surface appears promising, but the ET progress of membrane-bound hydrogenase is challenging due to the complex enzyme structure compared with soluble redox enzymes. Gutiérrez-Sánchez and coworkers demonstrated that the introduction of a phospholipidic bilayer on positively charged 4-ATP modified Au electrode surfaces effectively controls the orientation of membrane-bound [NiFeSe]-hydrogenase for hydrogen oxidation (Figure 8) [167]. The hydrophobic lipid tail of hydrogenase can be embedded into the phospholipidic bilayer, thereby reducing the orientation distribution and promoting the ET process. All these results suggest that SAM-modified electrodes are paramount to provide a versatile platform for understanding how to tune the right enzyme orientation for DET.

Conclusions and Perspectives
Redox proteins and enzymes immobilized on solid surfaces are fragile biomolecular entities. With a few exceptions, they retain ET of enzyme function only in prepared microenvironments that somehow emulate their natural aqueous/membrane reaction media. Self-assembled molecular monolayers with multifariously functionalized thiols are close to ideal SAM building blocks which have emerged as the core class over the last couple of decades. The -SH thiol end ascertains robust Au-S linking which is now increasingly well understood as dominated by the Au(0)-•S(0) gold-thiyl and strong van der Waals Au-S interaction. The opposite functionalized end of the SAM thiol molecules offers the choice of hydrophilic or hydrophobic, electrostatically charged or neutral, and structurally large or small terminal groups, well suited for designed gentle protein/enzyme linking to the solid SAM-modified electrochemical surface. The option of varying the length of the SAMforming molecules offers additional control of the electron tunneling process as well as of the dielectric and other local environmental properties crucial in the overall control of the electrochemical activity of the immobilized proteins or enzymes.
We have first overviewed the preparation and comprehensive characterization of thiol-based

Conclusions and Perspectives
Redox proteins and enzymes immobilized on solid surfaces are fragile biomolecular entities. With a few exceptions, they retain ET of enzyme function only in prepared microenvironments that somehow emulate their natural aqueous/membrane reaction media. Self-assembled molecular monolayers with multifariously functionalized thiols are close to ideal SAM building blocks which have emerged as the core class over the last couple of decades. The -SH thiol end ascertains robust Au-S linking which is now increasingly well understood as dominated by the Au(0)-•S(0) gold-thiyl and strong van der Waals Au-S interaction. The opposite functionalized end of the SAM thiol molecules offers the choice of hydrophilic or hydrophobic, electrostatically charged or neutral, and structurally large or small terminal groups, well suited for designed gentle protein/enzyme linking to the solid SAM-modified electrochemical surface. The option of varying the length of the SAM-forming molecules offers additional control of the electron tunneling process as well as of the dielectric and other local environmental properties crucial in the overall control of the electrochemical activity of the immobilized proteins or enzymes.
We have first overviewed the preparation and comprehensive characterization of thiol-based SAMs on Au surfaces in particular, both per se and as developed in redox protein/enzyme electrochemical research over the last couple of decades. Preparation is, in principle, straightforward, although the ultimate SAM properties depend in subtle ways on thiol exposure time, temperature, and other external controlling factors. A variety of sophisticated SAM surface techniques have brought the understanding of the fundamental molecular and electronic SAM structure to a high level, ranging all the way from the ordered domain right down to the single molecule. The techniques include spectroscopy (XPS, FTIR, SPR, Raman, NEXUS, and others), microscopy (AFM, STM in the electrochemical in situ/operando modes), and mass balance techniques (QCM), supported by large-scale electronic structure calculations [4,6,16,17,66].
Well-defined solid SAM-modified Au-and other electrode surfaces are a pre-requisite for the productive immobilization of bioelectrochemically active DET enzymes. An outstanding challenge is that polycrystalline Au-and other metallic electrodes are nearly always used, with single-crystal, atomically planar, e.g. Au (111), electrodes only relatively recently introduced as electrochemical biomolecular target surfaces. A variety of local low-and higher-index surface structures are distributed over the polycrystalline Au-and other surfaces, expectedly giving quite different surface ET activities [92]. This presents a challenge to the microscopic characterization of the pure and SAM-modified electrode surfaces but at the same time also provides new openings in the way of more robust protein/enzyme monolayers and higher enzyme activity, if the polycrystallinity can be controlled such as for NPG and other nanoporous metallic electrodes.
We have next overviewed and discussed adsorption and controlled protein and enzyme orientation on strategically chosen SAMs, of both simple ET metalloproteins (cyt. c and c 4 , azurin) and a variety of much more complex redox metallo-and nonmetalloenzymes (blue copper oxidases, FAD-based enzymes, cellobiose dehydrogenase, [FeS]-cluster dehydrogenases). We have shown that properly chosen SAMs can be brought to control efficiently the enzyme surface orientation in the ways most favorable both for productive bioelectrocatalysis and for detailed mapping of the molecular mechanisms involved. We have also shown that, although of more complex molecular/atomic surface structure, SAM-modified NPG and other nanoporous metallic electrochemical surfaces, structurally characterized to intermediate levels of resolution, may offer other advantages. These extend to increased enzyme stability and even enhanced catalytic efficiency compared to atomically planar electrode surfaces.
Overall, the present state of detailed structural and mechanistic protein and enzyme bioelectrochemical mapping has now advanced in impressive detail, approaching the true level of the single molecule [3,4,6,168,169] and supported by large-scale electronic structure computations [16,17,92,138]. With new and rapidly increasing understanding of the complex, heterogeneous, and anisotropic electrode/SAM/protein/enzyme/aqueous interface, its real exploitation in the strategic design and development of enzyme biofuel cells, next-generation bioelectrochemical sensors, and other high-technology applications is rapidly coming close.