Photocatalytic TiO 2 -Based Nanostructured Materials for Microbial Inactivation

: Pathogenic microorganisms can spread throughout the world population, as the current COVID-19 pandemic has dramatically demonstrated. In this scenario, a protection against pathogens and other microorganisms can come from the use of photoactive materials as antimicrobial agents able to hinder, or at least limit, their spreading by means of photocatalytically assisted processes activated by light — possibly sunlight — promoting the formation of reactive oxygen species (ROS) that can kill microorganisms in different matrices such as water or different surfaces without affecting human health. In this review, we focus the attention on TiO 2 nanoparticle-based antimicrobial materials, intending to provide an overview of the most promising synthetic techniques, toward possible large-scale production, critically review the capability of such materials to promote pathogen (i.e., bacteria, virus, and fungi) inactivation, and, finally, take a look at selected technological applications.


Introduction
Over the last decades, titanium dioxide (TiO 2 ) has been extensively investigated for its physical-chemical properties, that, combined with its high stability, low cost, and safety for the environment and humans, have resulted in a range of environmental and energy applications.
When TiO 2 is obtained at nanoscale, many relevant properties of this semiconductor are enhanced, due to the increased surface area, which results from the high surface-to-volume ratio, the excellent surface morphology, and the band edge modulation, that, overall, turn into a control on the photocatalytic behavior and performance of the nanostructured materials [1][2][3].
Upon illumination, TiO 2 nanoparticles (NPs) convert incoming photons into excitons, or electron/hole pairs, which can migrate to the surface and participate in redox reactions and generate reactive oxygen species (ROS), such as hydroxyl radicals (OH·) and superoxide(O 2 − ) [3,4].
Indeed, the photocatalytic behavior of TiO 2 NPs has been widely exploited for removal of water and air contaminants and self-cleaning surfaces [1][2][3][4]. Currently, increasing concerns regarding the COVID-19 pandemic are drawing the attention of researchers and general public more and more to photocatalytic antimicrobial and antiviral treatments with the purpose of hindering virus spreading, by using light (possibly solar light) activated systems. TiO 2 NPs are among the most studied materials in the area of photocatalytic antimicrobial applications, having demonstrated a great potential for the disinfection/inactivation of harmful Vargas et al., starting from the Ti(OBu)4 as a precursor, performed a sol gel synthesis consisting of two steps: firstly, the Ti(OBu)4 was dispersed in ethanol and allowed to stir in distilled water. Then, the obtained product was dried before being thermally treated for 2 h, at increasing temperature, 350 °C , 400 °C , and 770 °C. As a result, X-ray diffraction (XRD) analysis demonstrated that a different crystalline composition was attained as a function of the thermal treatment temperature. In particular, the obtained material was found amorphous after the treatment at 350 °C, while anatase was the main crystalline phase at 400 °C, and finally the treatment at 770 °C resulted in rutile as the single crystalline phase. Scanning (SEM) and transmission (TEM) electron microscopy analysis highlighted a morphology ( Figure 2) characterized by the presence of primary sub-micrometric spheroidal particles with different average sizes, namely 100 nm for the amorphous product, and 50 nm for the two crystalline samples. The thermal treatment conditions were found to also affect the specific surface area value [16] of the resulting nanomaterials, as the increase in temperature resulted in a decrease of the specific surface area [17].
Dell'Edera et al. pointed out the correlation between TiO2 heat treatment and the morphological properties of the obtained nanomaterial. The synthesis was carried out by precipitation of TiOSO4 in an aqueous solution of NH4HCO3. The obtained sol was thermally treated in an oven at 110 °C for 16 h. SEM

Sol-Gel Methods
The sol-gel method is based on the conversion of "sol(s)", namely solid particles suspended in a liquid, into a network of sols defined as "gel", containing both a liquid phase and a solid phase. Figure 1 shows a schematic representation of a general procedure typically followed for a common sol-gel synthesis. In the first step, the TiO 2 precursor is dissolved in water, in an alcohol (e.g., methanol, ethanol, or isopropanol) or in a defined mixture of alcohol and water as a reaction solvent. Commonly used TiO 2 precursors include (but are not limited to) titanium (IV) butoxide (Ti(OBu) 4 ), titanium (III) chloride (TiCl 3 ), titanium (IV) tetrachloride (TiCl 4 ), and titanium (IV) isopropoxide (Ti[OCH(CH 3 ) 2 ] 4 , TTIP). At this stage, the formation of TiO 2 takes place through the two main reactions of hydrolysis (Equation (1)) and condensation (Equations (2) and (3)). Subsequently, in most reported protocols, Catalysts 2020, 10, 1382 4 of 46 the obtained product is thermally treated in order to obtain TiO 2 NPs with a defined size and crystalline phase [12][13][14][15].
Hydrolysis: Ti(OR) 4 Ti(OH) 4 + Ti(OR) 4 →2TiO 2 + 4ROH (alcoxolation) In the reactions (1) and (3), R represents organic functional group of the organometallic TiO 2 precursor, such as ethyl, i-propyl, n-butyl. The dilution of the TiO 2 precursor in the alcohol and/or in water before being added to the selected reaction solvent is useful to control the reaction rate of the hydrolysis process by slowing down the reaction rate as the dilution of the precursors increase. In the sol-gel method, the synthesis parameters affecting the properties and structure (crystalline phase(s)) of the resulting TiO 2 NPs photocatalyst are: H 2 O/TiO 2 precursor ratio, pH value measured during the synthesis, and experimental conditions of possible calcination step.
Vargas et al., starting from the Ti(OBu) 4 as a precursor, performed a sol gel synthesis consisting of two steps: firstly, the Ti(OBu) 4 was dispersed in ethanol and allowed to stir in distilled water. Then, the obtained product was dried before being thermally treated for 2 h, at increasing temperature, 350 • C, 400 • C, and 770 • C. As a result, X-ray diffraction (XRD) analysis demonstrated that a different crystalline composition was attained as a function of the thermal treatment temperature. In particular, the obtained material was found amorphous after the treatment at 350 • C, while anatase was the main crystalline phase at 400 • C, and finally the treatment at 770 • C resulted in rutile as the single crystalline phase. Scanning (SEM) and transmission (TEM) electron microscopy analysis highlighted a morphology ( Figure 2) characterized by the presence of primary sub-micrometric spheroidal particles with different average sizes, namely 100 nm for the amorphous product, and 50 nm for the two crystalline samples. The thermal treatment conditions were found to also affect the specific surface area value [16] of the resulting nanomaterials, as the increase in temperature resulted in a decrease of the specific surface area [17]. In the sol-gel routes, the hydrolysis reaction, necessary for the formation of TiO2, is acid catalyzed [18,19]. It follows that pH is a critical parameter in the synthesis that has been also found to influence morphological and structural properties of the resulting nanomaterial. Indeed, Ibrahim et al. demonstrated that alkaline conditions (pH 9) promote anatase structure, while higher acidity (pH < 5) results in rutile phase [20].
On the other hand, Galkina et al. synthesized anatase and brookite nanocrystalline TiO2 using a sol-gel process by working at acid pH [21]. TTIP was dissolved in isopropylic alcohol and then added to an aqueous medium acidified by nitric acid. The obtained sol was used to modify cotton fibers, thus obtaining an Reprinted with permission from ref. [16].
Dell'Edera et al. pointed out the correlation between TiO 2 heat treatment and the morphological properties of the obtained nanomaterial. The synthesis was carried out by precipitation of TiOSO 4 in an aqueous solution of NH 4 HCO 3 . The obtained sol was thermally treated in an oven at 110 • C for 16 h. SEM micrographs (Figure 2 panel A-B) show that small NPs were present in the untreated sample (~1 nm); after the heat treatment larger NPs were visible (23 ± 6 nm). Furthermore, Figure 2 panel C shows that the crystalline phase changed and a trend of the crystalline phase composition was observed as a function of the extent of heat treatment conditions. Initially there was a predominance of the brookite phase, then, during the thermal treatment the brookite phase progressively converted into the anatase phase. The specific surface area (SSA) depended on the thermal treatment conditions; in particular, for untreated TiO 2 NPs an SSA of 503.0 m 2 /g was measured, while treated NPs resulted in an SSA of 336.5 m 2 /g. The obtained TiO 2 NPs were found to be photocatalytically active, thus a potential candidate for antimicrobial applications. The dependence of the photocatalytic performance on properties such as crystalline phase composition, surface chemistry, and surface area was also highlighted [16].
In the sol-gel routes, the hydrolysis reaction, necessary for the formation of TiO 2 , is acid catalyzed [18,19]. It follows that pH is a critical parameter in the synthesis that has been also found to influence morphological and structural properties of the resulting nanomaterial. Indeed, Ibrahim et al. demonstrated that alkaline conditions (pH 9) promote anatase structure, while higher acidity (pH < 5) results in rutile phase [20].
On the other hand, Galkina et al. synthesized anatase and brookite nanocrystalline TiO 2 using a sol-gel process by working at acid pH [21]. TTIP was dissolved in isopropylic alcohol and then added to an aqueous medium acidified by nitric acid. The obtained sol was used to modify cotton fibers, thus obtaining an antibacterial composite material. Cotton fibers were pre-treated in order to anchor the TiO 2 NPs therein. In particular, a spacer, a cyclic anhydride, prepared by the reaction between 1,2,3,4-butanetetracarboxylic acid (BTCA) and NaH 2 PO 2 in a water solution, was inserted at the fiber surface. Once pre-treated, the cotton fibers were functionalized with TiO 2 NPs by adding them to the aqueous solution and heating at 70 • C for 2 h. The presence of nitric acid in the preparation of the sol was found able to drive the NP growth toward the formation of both brookite and anatase, single phase, TiO 2 NPs. The obtained cotton/TiO 2 composites demonstrated high bacteriostatic effects against Escherichia coli (E. coli) [21].
The solvent used to disperse the TiO 2 precursor may also affect the physical-chemical characteristics of TiO 2 NPs prepared by using a sol-gel method. Indeed, the morphology of the TiO 2 NPs was found to be influenced by the volume ratio between alcohol and TiO 2 precursor, as well as by the nature of alcohol selected as a solvent for the specific synthetic route. As an example, Bahar et al. reported that syntheses performed in ethanol lead to larger NPs (33 nm) than those obtained in butanol (26 nm) and isopropanol (30 nm) [22]. Duymaz et al. investigated the effect of the solvent composition, testing two different solvents (ethyl alcohol or water) to dissolve the TiO 2 precursor. After preparing the precursor in water and alcohol solution, respectively, its oxidation was catalyzed by the addition of nitric acid to the two systems and, for both of them, the sol-gel reaction was carried out at 50 • C to obtain a homogeneous dispersion. Then, the TiO 2 gel was dried for 24 h at 90 • C and finally ground to obtain fine powders, which were analyzed using a dynamic light scattering (DLS) technique. The use of ethanol was found to induce the formation of aggregates of TiO 2 NPs smaller (1858 nm) than those obtained by using water (2641 nm). The report indicated that the smaller TiO 2 aggregates, thanks to their size, were found to be optimal for being supported on several substrates such as silica, calcite, talc, and zinc borate. The immobilization of TiO 2 on each of these substrates was performed directly in situ, by simply adding the supporting material in the reaction mixture where the oxidation reaction takes place. The obtained NPs showed antibacterial behavior against E. coli and Staphylococcus aureus (St. aureus) [23].

Hydrothermal Methods
Hydrothermal (HT) synthesis, along with sol-gel process, is also a very common method for preparing TiO 2 -based nanomaterials. In hydrothermal synthesis, that is, a solution approach, the formation of nanomaterials can take place in a wide range of temperatures, from room temperature to high temperatures (up to 400 • C [8]). Pressure can also be relevant in HT reaction, as it enables the control of morphology for the resulting nanomaterial. In a HT reaction, either low-pressure or high-pressure conditions can be applied, depending on the vapor pressure of the most abounded component of the reaction mixture under the investigated experimental conditions. Therefore, generally, a HT synthesis is carried out in an autoclave reactor, where it is essential to carefully control pressure and temperature.
The scheme of a typical HT synthetic procedure is depicted in Figure 1. In the first step, a mixture of water and TiO 2 precursor is prepared; then the obtained solution is transferred in the autoclave reactor, setting specific temperature pressure conditions. At this stage, the hydrolysis and condensation reactions take place, forming a three-dimensional network, similar to what happens in a sol-gel process. HT processes can be regarded as a sort of evolution of the synthetic sol-gel processes, because they allow one to obtain NPs with a high degree of crystallinity in fewer and simpler steps relative to the procedures typically realized for the sol-gel methods.
Rasheed et al. proposed a procedure to obtain rutile phase TiO 2 NPs starting from a sol obtained by mixing TiCl 4 , deionized water, and ethanol. The dispersion was kept under vigorous stirring for 30 min until it became colorless, then, it was introduced into a Teflon-lined stainless-steel autoclave. The autoclave was sealed and placed in an oven at 200 • C for 6 h. The XRD analysis confirmed the presence of rutile as a single crystalline phase in the resulting materials. The photocatalytic antibacterial behavior was investigated against E. coli and St. aureus [24].
Varying the synthetic parameter of the HT procedure was also possible to obtain nanomaterials with a more complex morphology, such as the flower like nanostructures, composed by rod-like TiO 2 NPs reported by Korosi et al. [25]. A sol dispersion was obtained by mixing distilled water and TiCl 4 previously dissolved in 2-propanol. The resulting colorless dispersion was aged for two weeks at room temperature without stirring and then left to react in autoclave, for 12 h at increasing temperature values, namely 150 • C, 200 • C, and 250 • C. The HT reaction temperature affected both the structure and the specific surface area of the obtained material. Indeed, as the temperature increased, a decrease of the specific surface area was observed, along with an increase of the rutile amount up to the 100% after treatment for 12 h at 250 • C [25]. Further, SEM micrographs ( Figure 3) pointed out that the crystallite size increased with the HT treatment temperature. The obtained material was effective in the photocatalytic inactivation of Klebsiella pneumoniae (K. pneumoniae).
and then left to react in autoclave, for 12 h at increasing temperature values, namely 150 °C, 200 °C , and 250 °C. The HT reaction temperature affected both the structure and the specific surface area of the obtained material. Indeed, as the temperature increased, a decrease of the specific surface area was observed, along with an increase of the rutile amount up to the 100% after treatment for 12 h at 250 °C [25]. Further, SEM micrographs ( Figure 3) pointed out that the crystallite size increased with the HT treatment temperature. The obtained material was effective in the photocatalytic inactivation of Klebsiella pneumoniae (K. pneumoniae). The HT process can also be conveniently tuned to modify the morphology of pre-synthetized TiO2 NPs, as reported by Leon-Rios et al., who demonstrated the conversion of spherical TiO2 NPs into TiO2 nanowires The HT process can also be conveniently tuned to modify the morphology of pre-synthetized TiO 2 NPs, as reported by Leon-Rios et al., who demonstrated the conversion of spherical TiO 2 NPs into TiO 2 nanowires obtained by means of a HT treatment. In detail, a water suspension of TiO 2 and NaOH was introduced in a Teflon-lined autoclave reactor and heated for 6 days at a temperature of 130 • C. The resulting powder was washed with HCl, then calcined at 500 • C leading to nanowires of TiO 2 in monoclinic phase, a metastable phase usually called TiO 2 -B. TiO 2 -B crystalline structure is characterized by a degree of exposure of TiO 2 (010) facets higher than that found in anatase, rutile, or brookite crystalline phase. Such TiO 2 (010) facets are known to be more photocatalytically active than other TiO 2 facets, thus making TiO 2 -B a valuable candidate for photocatalytic applications [26,27]. As an example, TiO 2 -B demonstrated effective in the UV induced inactivation of E. coli [27].

Preparation of TiO 2 /Metal Nanocomposites
The increasing demand for highly efficient, visible-light-active photocatalysts can be addressed by designing and realizing hybrid nanostructured materials formed of two or more components, each characterized by peculiar size dependent properties, surface chemistry, and morphology, that are combined into one nano-object with unprecedented chemical-physical characteristics. Indeed, the presence of a metallic domain coupled to TiO 2 leads to a nanomaterial particularly suited for accomplishing visible light photocatalysis, as, extending the absorption ability of TiO 2 in the visible, the capacity to convert solar energy into chemical energy is increased. Several papers have demonstrated a significant enhancement in visible-light-driven photocatalytic efficiency of nanocomposites obtained upon deposition of Ag and Au NPs onto TiO 2 nanostructures [3,4,28]. These composite materials are also very interesting due to the ability of metal NPs to convey bactericidal properties to the system even in the dark, thanks to the slow release of a small amount of metal ions that are known to be toxic for pathogens even at ppm level.

TiO 2 /Ag-Based Nanocomposites
One of the most investigated methods to improve the biocidal activity of TiO 2 against bacteria and viruses, enhancing at the same time its photocatalytic activity in the visible range, consists of modifying TiO 2 with Ag NPs thus obtaining TiO 2 /Ag-based nanocomposites with unique photocatalytic and antibacterial properties [29]. Remarkably, in metal/semiconductor-based nanocomposites the interaction between semiconductors and metal NPs, under UV or visible light irradiation affects the properties of the resulting nanocomposite [3]. Indeed, the role played by the metal NPs in influencing Catalysts 2020, 10, 1382 8 of 46 the energetics of the system, leading to an enhanced photoinduced charge separation, has been extensively investigated especially with respect to the photocatalytic reactions [30].
Several methods have been reported for the preparation of TiO 2 /Ag-based nanocomposites with combined antibacterial and photocatalytic properties. Sol-gel processes, that allow one to simultaneously synthesize TiO 2 NPs and Ag NPs, are among the most used methods for preparing TiO 2 /Ag-based nanocomposites. In particular, the extensively investigated sol-gel synthetic protocols produce Ag NPs by reduction of Ag salt (typically AgNO 3 or AgClO 4 ) with a suitable reductant, such as ascorbic acid, glucose, sodium borohydride and citrate. Kedziora et al. reported a method for obtaining TiO 2 /Ag-based nanocomposites with a control on the amount of Ag. The synthetic procedure was based on two steps: the preparation of the Ag precursor and the sol-gel process. Firstly, a diamminosilver (I) nitrate solution was prepared by adding potassium hydroxide in a silver nitrate solution, resulting in a brown precipitate that was then dissolved in ammonium hydroxide. In the second step, first titanium n-butoxide was added drop wise in acetone, next the diamminosilver (I) and the reducing agent (glucose) were introduced in the reaction vessel obtaining a sol. Afterward, the sol was washed and dried at 80 • C and finally calcinated at 400 • C for 10 h to obtain TiO 2 in anatase phase. This process made it possible to control the amount of Ag NPs nucleating on the TiO 2 surface by simply repeating the process of impregnation with diamminosilver (I) nitrate solution and reduction by glucose. The TEM micrograph of TiO 2 /Ag nanostructures in Figure 4 points out the presence of spherical Ag NPs, appearing as high contrast round-shaped spots, in close contact with the surface of the lighter contrast TiO 2 structures [31].  Another approach for the preparation of TiO2/Ag nanocomposites is based on a double step synthesis, consisting of the reduction AgNO3 in presence of TiO2 NPs previously prepared by a HT method. In this case, the obtained nanocomposite was deposited on leather for antimicrobial function in the footwear industry. The antimicrobial activity was successfully demonstrated against the bacteria Pseudomonas aeruginosa (P. aeruginosa), St. aureus, and the fungi Candida albicans (C. albicans) [32]. Moreover, other reports indicated that a post-synthesis treatment carried out at high temperature (up to 500 °C) on TiO2 NPs before growing Ag NPs resulted in a nanocomposite with a higher crystallinity of the TiO2 phase in nanocomposite prepared for inactivation of St. aureus, E. coli, and Bacillus cereus (B. cereus) [33][34][35].
Another synthetic method, widely used for the production of TiO2 nanocomposites for antibacterial applications, relies on photo-reduction of Ag salt by UV radiation onto pre-synthesized TiO2 NPs. Skorb et al. synthesized TiO2-based nanocomposites using this method. Firstly, nanostructured TiO2 was synthetized by means of a sol-gel process, and deposited by spray-coating onto different substrates such as glass microscope slides or glazed ceramic. Afterwards, the deposited material was treated at 200 °C. Subsequently, Ag NPs were deposited on the surface of TiO2 by dipping the substrate in aqueous solution of AgSO4 and irradiating with a UV lamp for 10 s. A further step allowed the preparation of TiO2/Ag/Ni nanocomposites by electrodeposition of a nickel layer onto a surface of TiO2/Ag from a Ni(CH3COO)2 solution. The antimicrobic activity of the nanocomposite was assessed against Pseudomonas fluorescens (P. fluorescens) and Lactococcus lactis (Lc. lactis) [  Another approach for the preparation of TiO 2 /Ag nanocomposites is based on a double step synthesis, consisting of the reduction AgNO 3 in presence of TiO 2 NPs previously prepared by a HT method. In this case, the obtained nanocomposite was deposited on leather for antimicrobial function in the footwear industry. The antimicrobial activity was successfully demonstrated against the bacteria Pseudomonas aeruginosa (P. aeruginosa), St. aureus, and the fungi Candida albicans (C. albicans) [32]. Moreover, other reports indicated that a post-synthesis treatment carried out at high temperature (up to 500 • C) on TiO 2 NPs before growing Ag NPs resulted in a nanocomposite with a higher crystallinity of the TiO 2 phase in nanocomposite prepared for inactivation of St. aureus, E. coli, and Bacillus cereus (B. cereus) [33][34][35].
Another synthetic method, widely used for the production of TiO 2 nanocomposites for antibacterial applications, relies on photo-reduction of Ag salt by UV radiation onto pre-synthesized TiO 2 NPs. Skorb et al. synthesized TiO 2 -based nanocomposites using this method. Firstly, nanostructured TiO 2 was synthetized by means of a sol-gel process, and deposited by spray-coating onto different substrates such as glass microscope slides or glazed ceramic. Afterwards, the deposited material was treated at 200 • C. Subsequently, Ag NPs were deposited on the surface of TiO 2 by dipping the substrate in aqueous solution of AgSO 4 and irradiating with a UV lamp for 10 s. A further step allowed the Catalysts 2020, 10, 1382 9 of 46 preparation of TiO 2 /Ag/Ni nanocomposites by electrodeposition of a nickel layer onto a surface of TiO 2 /Ag from a Ni(CH 3 COO) 2 solution. The antimicrobic activity of the nanocomposite was assessed against Pseudomonas fluorescens (P. fluorescens) and Lactococcus lactis (Lc. lactis) [36].

Coupling TiO 2 with Other Metals and Metal Oxides
Current research on TiO 2 -based nanomaterials for antibacterial applications is also focusing on valid and cost-effective metal NPs, as alternative to Ag, to merge to TiO 2 , possibly characterized also by intrinsic antibacterial properties.
Kaushik et al. developed a TiO 2 /Al-based nanocomposite material with antibacterial activity. The synthetic method is essentially a HT process performed in the presence of an Al-based compound. Firstly, titanium (IV) isopropoxide (TTIP) and ethylene diamine were mixed in isopropyl alcohol, next aluminum isopropoxide was added, and finally the solution was transferred to Teflon-lined stainless-steel autoclave and kept at 180 • C for 18 h in oven. The obtained material was found to consist of Al doped TiO 2 NPs, giving Al the twofold role of (i) extending the visible range the wavelengths suitable to activate the photocatalytic properties and (ii) significantly increasing the antibacterial activity with respect to undoped TiO 2 . Higher bacterial disinfection activity of the doped samples compared to that of the undoped TiO 2 was observed under visible light irradiation (nearly 80%) as well as in the dark (nearly 20%) against both St. aureus and E. coli bacteria [37].
Venieri et al. studied the bactericidal activity of TiO 2 -based nanostructures doped with Co and Mn for inactivation of K. pneumoniae and E. coli. They synthesized by sol-gel Mn, Co, and Mn:Co doped TiO 2 . Each nanocomposite was synthesized starting from a sol containing both the TiO 2 and the dopant precursor, afterward the pH of the sol was adjusted to pH 7, and finally, the resulting nanocomposite materials were washed with water and thermally treated at 500 • C for 3 h [38][39][40].
An original approach developed to improve both the antibacterial activity and the photocatalytic properties was based on the use of rare earth metals as dopants for TiO 2 NPs. In particular, Nd is a lanthanide element that attracted a lot of attention, due to its unique optical and magnetic properties, that make it extremely useful for application in optoelectronic and magnetic devices [41]. Nithya et al., produced Nd doped TiO 2 NPs by means of a sol-gel method for inactivation of E. coli and St. aureus. TTIP and Nd (III) acetate dehydrate were dissolved in isopropyl alcohol at pH 9, afterwards acetic acid was added to complete the hydrolysis reaction. Then, the obtained powder was dried at 120 • C for 2 h and annealed at 600 • C for 3 h [42].
Another effective strategy to increase the antibacterial ability of nanostructured TiO 2 relies on the coupling with a suitable metal oxide semiconductor.
Siwińska-Stefańska et al. synthetized various TiO 2 /ZnO binary semiconductor nanocomposites, with different TiO 2 :ZnO ratios, by using HT route. In particular, the synthesis of the binary TiO 2 /ZnO semiconductor was carried out by mixing TTIP, dissolved in alcohol, with zinc acetate, dissolved in water. The obtained mixture was heated in autoclave at 160 • C. All the synthesized TiO 2 /ZnO binary semiconductors showed a well-defined crystalline structure and a high surface area [43]. The nanocomposite demonstrated antibacterial activity against seven different bacteria including E. coli, P. aeruginosa, St. aureus, a methicillin-resistant St. aureus, B. cereus, Bacillus licheniformis (B. licheniformis), and anaerobic Clostridium perfringens (C. perfringens).
Other hybrid nanocomposites, such as TiO 2 :In 2 O 3 , TiO 2 :g-C 2 N 4 , and TiO 2 :SiO 2 are currently under investigation due to their promising photoactivity and antibacterial properties [36,[44][45][46][47]. For instance, TiO 2 :In 2 O 3 photocatalysts were prepared by sol-gel route by reacting TiCl 4 and In(NO 3 ) 3 in water and then stabilized by addition of HNO 3 . The obtained photocatalyst was able to inactivate P. fluorescens and Lc. lactis [36]. TiO 2 :g-C 2 N 4 synthesis involved the growth of TiO 2 nanosheets with (001) facets exposed by a hydrothermal route based on the decomposition of TiO(Bu) 4 in the presence of g-C 2 N 4. The TiO 2 :g-C 2 N 4 nanocomposite was able to inactivate E. coli [44]. Mesoporous silica nanospheres functionalized with TiO 2 were prepared in a three-step procedure. The first step was conducted according to a modified Stöber sol-gel method involving the hydrolysis of tetraethyl orthosilicate (TEOS) in alkaline conditions. In a second step, a mesoporous external layer was deposited on the silica spheres by letting TEOS react in the presence of CTAB (Cetyltrimethylammonium Bromide). Finally, functionalization with TiO 2 was accomplished by means of the reaction of titanium (IV) butoxide at the mesoporous silica nanosphere surface. The photocatalytic antibacterial properties of TiO 2 :SiO 2 were investigated against E. coli [45].
Other innovative materials recently proposed for their antimicrobial properties are La-doped TiO 2 /calcium ferrite/diatomite and TiO 2 /chitosan/graphene oxide (GO@CS@TiO 2 ) [46,47].The former was prepared by reacting ferric nitrate nonahydrate and calcium nitrate tetrahydrate in the presence of diatomite. In the next step, tetrabutyl orthotitanate and lanthanum nitrate hexahydrate were left to react in the presence of calcium ferrite/diatomite finally leading to La-doped TiO 2 /calcium ferrite/diatomite that was able to kill E. coli under visible light irradiation [46]. For preparing GO@CS@TiO 2 , in brief, GO was dispersed in ultrapure water, then CS was dissolved in acetic acid and added to GO solution obtaining nanometer film. Finally, GO@CS@TiO 2 nanocomposites were prepared by adding colloidal TiO 2 to the GO@CS film. GO@CS@TiO 2 antibacterial properties were investigated against Aspergillus niger (A. niger) and Bacillus subtilis (B. subtilis) [47].
Finally, examples of bio-hybrid nanocomposites have also been reported. For instance Kim et al.

Activity of TiO 2 -Based Nanostructured Materials against Bacteria, Fungi, and Virus
This section intends to provide an overview of the antimicrobial photocatalytic activity of the TiO 2 NPs against bacteria, virus, and fungi, critically discussing the numerous parameters affecting the experimental results reported in literature. A large number of experimental variables have been found to affect the efficiency of photocatalytic TiO 2 -based NPs in the inactivation of microorganisms. The main parameters considered in the following are structural characteristics of the photocatalytic TiO 2 -based materials, morphology of bacteria such as structural, genomic, and physiological features, their metabolism, environmental conditions such as absence/presence of a light source, and environmental stress. Moreover, specific attention will be devoted to the intrinsic antibacterial activity of TiO 2 and TiO 2 -based nanocomposite. The antiviral and antifungal activity of TiO 2 nanostructures will be also presented.
As a general scheme, the toxicity mechanism of TiO 2 to pathogenic microorganisms could be summarized as follows ( the intrinsic antibacterial activity of TiO2 and TiO2 -based nanocomposite. The antiviral and antifungal activity of TiO2 nanostructures will be also presented. As a general scheme, the toxicity mechanism of TiO2 to pathogenic microorganisms could be summarized as follows ( Figure 5): (A) cell damage and lipid peroxidation due to NPs attachments by electrostatic interaction on cell wall, (B) breaking of cytoplasmic flow due to NPs obstruction of nutrients carrier, photocatalytic degradation to (C) biological macromolecular, and (D) intracellular organelles. Remarkably, the literature reported experimental results and their interpretation often appear debated and sometimes are in disagreement; therefore, here we aim to illustrate and critically discuss. The criteria identified to select the reported studies are the nanosize regime of the investigated TiO2 based materials (average sizes in the range 1-100 nm), the analysis of multiple experimental parameters, and the techniques to evaluate the antimicrobial activity of TiO2 NPs.

Effect of TiO2 Nanostructure Characteristics
The antibacterial effect of TiO2 NPs-based nanomaterials was investigated as a function of their chemicalphysical characteristics, which include NPs crystalline structure, particle size and shape, doping, and presence of co-catalysts such as metallic NPs and metal oxide NPs. Lin et al. studied the antibacterial effect of five types of TiO2 NPs, namely pure anatase NPs with average size of 10, 25, and 50 nm, 50 nm rutile NPs, and both anatase and rutile NPs with an average size of 25 nm. The photocatalytic experiments were performed under Remarkably, the literature reported experimental results and their interpretation often appear debated and sometimes are in disagreement; therefore, here we aim to illustrate and critically discuss. The criteria identified to select the reported studies are the nanosize regime of the investigated TiO 2 based materials (average sizes in the range 1-100 nm), the analysis of multiple experimental parameters, and the techniques to evaluate the antimicrobial activity of TiO 2 NPs.

Effect of TiO 2 Nanostructure Characteristics
The antibacterial effect of TiO 2 NPs-based nanomaterials was investigated as a function of their chemical-physical characteristics, which include NPs crystalline structure, particle size and shape, doping, and presence of co-catalysts such as metallic NPs and metal oxide NPs. Lin et al. studied the antibacterial effect of five types of TiO 2 NPs, namely pure anatase NPs with average size of 10, 25, and 50 nm, 50 nm rutile NPs, and both anatase and rutile NPs with an average size of 25 nm. The photocatalytic experiments were performed under natural sunlight testing the NPs antibacterial activity against E. coli cells. The study was carried out on aqueous solutions at different pHs and ionic strengths. The smallest anatase NPs showed the highest affinity with cell surface in presence of TiO 2 NPs, 50 mg/L, 3 h, and were, thus, found to induce higher oxidative cell damage. Interestingly, pH and ionic strength of the aqueous solution were also found to affect the antibacterial activity of the NPs ( 1 Figure 6. TEM images showed NPs-type-dependent bacterial cell membrane localizations of TiO 2 NPs and the morphological changes of the cell exposed to the NPs: TEM images of the unsliced (A-F) and sliced (G-L) Escherichia coli samples: A and G untreated; B and H upon treatment with 10 nm anatase TiO 2 NPs; C and I upon treatment with 25 nm anatase TiO 2 NPs; D and J upon treatment with 50 nm anatase TiO 2 NPs; F and L upon treatment with 50 nm rutile TiO 2 NPs; with all samples having been exposed to natural sunlight. The blue arrows highlight the cells and the red arrows point to the NP aggregates. Reprinted with permission from ref [55].
Shirai et al. compared the antibacterial activity of anatase TiO 2 microparticles (5 µm) and anatase TiO 2 NPs (21 nm). In particular, they studied the extent of antimicrobial effect on switching off the UV light irradiation. The antibacterial activity observed was higher for the 21 nm TiO 2 NPs than the 5 µm TiO 2 microparticles, even 6 h after stopping the UV exposure [56].
In the case of deposited nanostructured TiO 2 -based materials, the resulting antibacterial activity may also be affected by the characteristic of the nanostructured coating. Indeed, TiO 2 NPs deposited on polyethylene by HIPMS (high power impulse magnetron sputtering), a pre-treatment of the polyethylene substrate with a RF-plasma (radio frequency-plasma) results in a roughness suitable for improving adhesion of bacterial cells to the substrate, that was found effective in enhancing the efficiency of the photocatalytic TiO 2 -assisted bacteria inactivation [57].
Sunada et al. demonstrated the bactericidal activity on E. coli and the decomposition ability on its endotoxin (detoxifying activity) of silica thin film (100 nm) modified with TiO 2 . Endotoxin is a toxic lipopolysaccharide (LPS) cell wall constituent of Gram-negative bacteria. The authors investigated the effect of TiO 2 film photocatalysts on the concentration of endotoxin and survival ratio of E. coli, and found that while the survival ratio of the bacteria decreased either in presence and in absence of the TiO 2 film (due to the germicidal effect of UV light), only in presence of TiO 2 film was there also a decrease of the amount of endotoxin observed, thus highlighting the effect of the TiO 2 photocatalyst on the integrity of the outer membrane of the cells [58].
In addition, modification of TiO 2 nanostructures with metal NPs (Ag, Cu, Ce, Al, In, Mn, and Co), being able to improve the absorption property in the visible of TiO 2 NPs-based nanocomposites, can enhance the efficiency in generation of ROS and, thus, increase the antimicrobial effect. In addition, the metal ions released from the metal NPs, once up taken by cells through their membrane, may interact with functional groups of proteins and nucleic acids, resulting in relevant damage of enzymatic activity, alteration of the cell structure, modification of the normal physiological processes, and, ultimately, in the inactivation of the microorganism [36][37][38]51,[59][60][61].
Liu et al. studied antibacterial activity of a P/Ag/Ag 2 O/Ag 3 PO 4 /TiO 2 photocatalyst against E. coli under LED lamp irradiation. The authors observed a bacterial inactivation of 100% by the composite within 20 min of photocatalytic treatment. On the contrary, under dark conditions, a reduction of E. coli was achieved (2-log cycles), attributed to the amount of metal Ag loaded on the composite. The concentration of bacteria remained almost equal in presence of TiO 2 (in both the dark and light conditions). The inactivation process was also investigated by SEM analysis to evaluate cell damage. After 20 min of photocatalytic treatment, changes on the wall surface were observed, namely an increase in roughness or occurrence of holes in the cell wall. After 40 and 90 min, larger pits and holes were observed, and, subsequently the cells were found to be completely decomposed [62].
In general, metal NPs can improve the antibacterial activity both because they are able to enhance photocatalytic performance of TiO 2 and release metal ions, therefore they exert an intrinsic biocidal effect interacting with cells.

Effect of the Cell Membrane Structure
Several types of bacteria were investigated in order to elucidate the antimicrobial activity of TiO 2 NPs and TiO 2 NPs-based nanocomposites. In this perspective, E. coli, as a model for Gram-negative bacteria, and St. aureus or P. aeruginosa, as models for Gram-positive bacteria, have attracted a lot of attention.
The need of investigate the behavior of both Gram-positive and Gram-negative bacteria in response to the photocatalysis-promoted antibacterial properties of TiO 2 NPs arises from the intrinsic differences existing between these two classes of cells in terms of cell membrane structure, that may be responsible for a different interaction with TiO 2 -based NPs. In Figure 7, the difference between the cell walls of Gram-positive and -negative bacteria is shown.
St. aureus or P. aeruginosa, as models for Gram-positive bacteria, have attracted a lot of attention.
The need of investigate the behavior of both Gram-positive and Gram-negative bacteria in response to the photocatalysis-promoted antibacterial properties of TiO2 NPs arises from the intrinsic differences existing between these two classes of cells in terms of cell membrane structure, that may be responsible for a different interaction with TiO2-based NPs. In Figure 7, the difference between the cell walls of Gram-positive andnegative bacteria is shown.  For this reason, here, the cell membrane differences between Gram-positive and Gram-negative bacteria will be discussed first, and then relevant studies describing the TiO 2 NPs antibacterial activity as a function of the characteristics of the bacterium membrane structure will be reviewed.
The cell wall of Gram-negative bacteria is characterized by the following features: (i) the presence of an outer membrane (OM) that contains phospholipids in the inner leaflet and glycolipids, mainly lipopolysaccharide (LPS) in the outer leaflet; (ii) a thin peptidoglycan cell wall, consisting of repeated units of disaccharide N-acetyl glucosamine-N-actyl muramic acid, that are cross-linked by pentapeptide side chains; and (iii) a cytoplasmic-or inner-membrane (IM), formed of a phospholipid bilayer.
The intermembrane space between the OM and the IM is characterized by a compartment defined as periplasm, that essentially contains proteins [63]. The OM, thanks to its unique composition, represents a selective barrier, that effectively protects cell from many agents, including detergents and antibiotics, still allowing penetration of specific macromolecules. Furthermore, the OM provides structural stability to the cell [64]. Gram-positive bacteria, instead, lack OM, but possess a 30-100 nm multilayer of peptidoglycans, which is thicker than that present in Gram-negative bacteria, which is a few nm thick [65].
Importantly, the cell wall of Gram-positive bacteria is porous, as the peptidoglycan multilayer can be easily crossed by teichoic acids, formed of glycerol and glucosyl or ribitol phosphate. The teichoic acids are anionic glycopolymers, that provide a high density of negative charge at the surface of the wall, thus making Gram-positive bacteria more prone than Gram-negative bacteria to interacting with positively charged NPs [65].
Several authors considered the ensemble of these features essential to explaining the observed antibacterial activity of TiO 2 -based NPs being higher against Gram-positive than Gram-negative bacteria.
Fu et al. studied the effect of photocatalytic activity of TiO 2 NPs at different concentrations under ambient light towards the Gram-negative E. coli and the Gram-positive Bacillus megaterium (B. megaterium). While E. coli was found to be inhibited by 5 mM TiO 2 NPs, the highest tested concentration, B. megaterium was already inhibited at 1 mM [66].
Page et al. compared the antibacterial effect of TiO 2 and Ag-doped TiO 2 (being TiO 2 in anatase phase in both cases) NPs-based coatings against E. coli (Gram-negative) and St. aureus and B. cereus (Gram-positive bacteria) under UV radiation (254 nm) for 30 min. Both coatings demonstrated photocatalytic and antimicrobial properties; however, the Ag-doped TiO 2 NPs-based coating was found more effective against the Gram-positive bacteria than the bare TiO 2 NPs-based one. Since the peptidoglycan multilayer forming the cell wall, along with the other mentioned components, consists of an open polymers network with peptide bridges, indeed the photogenerated hydroxyl radicals more easily interact and damage the cell wall, as they are not hindered by the surface appendages, that are, instead, present at the E. coli surface OM [34].
A similar behavior was observed by Alizadeh Sani et al. The antibacterial activity of a nanocomposite film, composed of whey protein isolate and cellulose nanofibers, 1.0% (w/w) TiO 2 and 2.0% (w/v) rosemary essential oil, was evaluated and a growth inhibition effect was shown to be higher for Gram-positive bacteria, as Listeria monocytogenes (L. monocytogenes) and St. aureus, than Gram-negative bacteria, as E. coli, Salmonella enterica, and P. fluorescens [67].
Nakano et al. evaluated the microbiocidal activity of photocatalysts for various species (virus, Gram-negative and -positive bacteria). Bactericidal activity of TiO 2 coated glass under UV-A irradiation for 48 h was investigated and the author confirmed that Gram-bacteria cocci (GPC) (S. aureus and Enterococcus spp.) were promptly inactivated, while Gram-negative cocci (GNC) (E. coli and P. aeruginosa) were only gradually inactivated [68].
On the contrary, other authors showed that Gram-positive bacteria are more resistant than Gram-negative bacteria. Khezerlou et al. proposed that, due to its composition, the Gram-negative bacteria OM is an attractive target for hydroxyl radicals, produced upon the TiO 2 NPs photoactivation, that can react with the lipidic components of the membrane rather than crossing it. The authors inferred an antibacterial action consisting of two steps: in the first, the OM is compromised, being then involved the cytoplasmic membrane. In the second step, once the OM is breached, the radicals can disrupt cytoplasmic membrane and subsequently induce the cell death [5]. Backhaus et al. (2010) investigated the effect of TiO 2 P25 on two faecal indicator strains, E. coli and Enterococcus faecalis (Ent. faecalis), in real wastewater treatment plant effluents, that is a water matrix with total organic carbon (TOC) values in the range of 8-25 mg C /L and conductivity in the range 450-750 µS/cm (effluent organic matter, EfOM). By using a UV-A bulb lamp, presenting its highest emission at 365 nm, the authors observed a different response of two types of bacteria to the photocatalytic treatment: Ent. faecalis was less sensitive than the E. coli to the photocatalytically generated TiO 2 NPs. Such a result was accounted for by the occurrence of the thick peptidoglycan multilayer in Gram-positive bacteria, that might have a higher affinity with EfOM relative to the peptidoglycan layer characterizing Gram-negative bacteria (EfOM protecting Ent. faecalis by ROS) [69]. Pal et al. reached the same conclusions upon testing the antibacterial activity of TiO 2 P25 NPs on different species, such as E. coli and P. fluorescens (as Gram-negative bacteria), and B. subtilis, Microbacterium spp., Microbacteriaceae, and Paenibacillus sp. (as Gram-positive bacteria). The authors used an experimental set-up, consisting of an irradiation source (UV-A, 365 nm) incident on the bacterial suspension (in contact with a filter loaded with a defined amount of TiO 2 NPs) by clamping the light source on top of a suitable support, and found that photocatalytic inactivation was more effective against E. coli than against B. subtilis [70].
Other studies, conversely, observed a negligible difference between two microbial groups. Ripolles-Avila et al. tested two types of commercial TiO 2 NPs, the NM105 (Degussa, Frankfurt, Germany) and the NM101 (Institute for Health and Consumer Protection at the European Commission Joint Research, Ispra, Italy), the former being based on anatase phase 7 nm NPs, while the latter is a mixture of 21 nm anatase and rutile phase (80:20 wt/wt) NPs. The experiments performed to investigate the antibacterial activity of the NM105 and the NM101 were carried out both in the dark and under UV light irradiation (315-400 nm) on Salmonella enterica var. Enteritidis, E. coli, St. aureus, and B. cereus. The results indicated that the antibacterial activity was affected by the amount of TiO 2 NPs, both in the dark and under UV-light irradiation. Further, no significant difference, in terms of cell viability, was observed among the different investigated microbes [71]. Analogous results were obtained for TiO 2 NP-coated implants [72] or colloidal dispersions of TiO 2 NPs [73]. In summary, the authors claim that the photocatalytically assisted antibacterial activity of TiO 2 NPs, defined in terms of cell inhibition, does not depend on the features of cell membrane structure. Indeed, Haider et al. used TiO 2 in a self-cleaning transparent coating for windows in outdoors applications, preparing NPs by using TiCl 4 as a precursor, and calcinating at different, increasing temperatures (400 • C, 600 • C, 800 • C and 1000 • C), testing E. coli and P. aeruginosa. SEM analysis showed that after 2 h of sunlight irradiation, the TiO 2 NPs-based coating was effective against both bacteria, as no cells survived [74].
In this case, the authors also concluded that the cell wall structure was not the main parameter involved in bacterium resistance to photocatalysis-promoted antibacterial activity of TiO 2 NPs.

Effect of Bacterial Metabolism
The mechanisms behind cell growth inhibition and bacterial death, induced by the photocatalytic reactions promoted by TiO 2 NPs, have also been analyzed considering two different cell metabolisms. Skorb et al. investigated the effect of the photocatalytic activity of several TiO 2 NPs-based nanocomposites, on two bacteria strains showing a different metabolism: P. fluorescens, obligate aerobe, and Lc. lactis, facultative anaerobe. The investigated TiO 2 NPs-based nanomaterials, which consisted of thin-films of TiO 2 , TiO 2 :In 2 O 3 , TiO 2 /Ag, or TiO 2 /Ag/Ni deposited on a ceramic substrate, were prepared first by spraying the oxide sols, and then performing silver photo-deposition and electroless nickel deposition in order to convey antibacterial properties. According to the results, the differences between P. fluorescens and Lc. lactis in terms of bacteria inactivation rate could be associated to the morphologies of cell envelops and to the resistance of OM to radicals generated from the photocatalytic reactions [36]. In particular, P. fluorescens uses the oxidative phosphorylation to store energy required for cell respiratory functions. Therefore, for this type of cell, the increase of ion permeability of the cytoplasmic membrane, due to the damage induced by ROS, causes the loss of the proton gradient and consequently inhibits bacteria respiratory function. Lc. lactis (facultative anaerobe), instead, stores energy thanks to other metabolic process (such as lactic acid fermentation). For this reason, the damage on the cytoplasmatic membrane detected in Lc. lactis was scarcely relevant [36].

Effect of Physiological State of Bacteria Cell and Environmental Stress
Rincón et al. claimed that in photocatalytic bacteria inactivation experiments, the rate of cell inactivation can be affected by the bacterial growth state (exponential or stationary phase) as well as by generation state of the culture. Indeed, E. coli cells, collected during the exponential step, were inactivated in a shorter timeframe (1 h) with respect to the same cells collected during the growth stationary phase, that, instead, required 2.5 h to be completely inactivated in presence of P25 TiO 2 NPs under simulated sunlight irradiation. Moreover, E. coli cells from the third generation were found more resistant compared to those from the seventh generation.
The authors suggested that the performance of the TiO 2 assisted photocatalytic inactivation was influenced by physiological state and generation of the bacteria [75]. In detail, environmental stress was thought able to induce expression of genes involved in the synthesis of a specific set of proteins, that have been found responsible for mutations in the following generations. In addition, the stationary phase response to environmental changes induces the synthesis of the proteins that convey to E. coli resistance to several types of stress (i.e., heat shock, oxidation-UV light-, hyperosmolarity, and acidity). During the stationary phase, the expression of rpoS is also induced, which encodes a regulator for expression of genes [76,77], such as those involved in the protection against oxidants (e.g., catalase) and in the reparation of oxidative damage (e.g., exonuclease III) [78,79].
Another important factor is represented by the ability of bacteria to resist to environmental stress. Upon environmental stress, the bacteria can activate several mechanisms of defence, in order to limit the damage. Indeed, it is well known that there are specific enzymatic systems able to eliminate ROS. In bacteria such as St. aureus or Staphylococcus pyogenes (St. pyogenes), anti-ROS mechanisms, often associated to pathogen virulence, were identified [80,81]. Furthermore, bacteria are able to produce a biofilm that prevents the penetration of NPs into the cell membrane, thus hampering their antibacterial functions.
Interestingly, in TiO 2 NPs-assisted photocatalytic reactions, the concentration of ROS physiologically produced in presence of bacteria is another important parameter to be taken into account when considering cell damage [82]. Bonnet  St. aureus was found to be more resistant than the other bacteria under the investigated experimental conditions. Such evidence was explained in terms of the detoxification ability of the catalase activity, which is typical of this bacterium, and that was exerted in presence of TiO 2 NPs. Indeed, catalase is able to trigger the oxidative stress-related mechanisms against endogenous ROS (such as H 2 O 2 ), metabolites naturally produced by these cells, that consequently need to remove them by activating detoxification mechanisms; the same mechanisms can thus act against TiO 2 NPs-induced ROS [83]. These features, therefore, suggest that the resistance may be species-dependent.

Intrinsic Antibacterial Activity of TiO 2
The intrinsic antibacterial activity of TiO 2 NPs in the dark is an extremely controversial and debated issue, that, in fact, often hinders the use of TiO 2 NPs in any kind of application that does not allow light irradiation. Erdem and other authors [84,85] confirmed that different types of TiO 2 NPs were able to promote a short-term bacteria inactivation, even in the dark. In detail, in this case a possible killing mechanism is assumed to mainly involve the direct interaction between TiO 2 NPs and bacterial membrane. Indeed, the OM of Gram-negative bacteria (especially in E. coli) contains porins, which are membrane proteins acting as pores (1.5-2 nm in diameter) enabling diffusion of molecules in the cytoplasmic compartment. When the TiO 2 NPs and bacteria are in close contact, NPs may obstruct such pores, thus hindering the diffusion channels, preventing nutrition uptake, finally inducing cell death.
The close contact between TiO 2 NPs and E. coli OM was identified by Kiwi et al. as an essential condition, responsible for the intrinsic antibacterial activity of TiO 2 NPs in the dark. Such a conclusion was reached by monitoring E. coli inactivation experiments by TEM microscopy. Indeed, TEM micrographs, collected at different inactivation times, pointed out that, at suitable TiO 2 NPs amounts and at a pH close to its isoelectric point, aggregates of TiO 2 NPs migrate towards the OM of E. coli, where they accumulate due to electrostatic interactions, finally leading to extensive damage of the OM and therefore to the loss of E. coli cultivability [86][87][88]. Ripolles-Avila et al. investigated the antibacterial activity of TiO 2 NPs both in the dark and under UV-light irradiation. The test performed against both Gram-negative and -positive bacteria demonstrated an intrinsic bactericidal activity of TiO 2 NPs, since no significant differences, in terms of cell inactivation rate was observed with or without irradiation [71]. However, the presence of NPs was demonstrated to affect the bacteria growth, especially at low cell population density [89].
The antibacterial effect of P25 TiO 2 NPs was also investigated by Carré et al. (2014) against E. coli, specifically considering lipid peroxidation phenomena and performing a proteomic analysis. The extent of lipid peroxidation, through the thiobarbituric acid (TBA) assay, was found to significantly increase both in the dark and under UV-A-light irradiation, after 60 min exposure to TiO 2 P25 at a concentration of 0.4 g/L. The proteome of bacteria was investigated by means of electrophoresis , that is able to identify the extent of protein damage under different experimental conditions, namely at two distinct TiO 2 NPs concentration (0.1 or 0.4 g/L) with and without UV-A irradiation, and evaluated against two control tests performed without catalyst, with and without irradiation, for 30 min. In particular, in presence of TiO 2 NPs, both in the dark and under UV-A irradiation, OM proteins suffered most alterations, probably due to the direct contact with TiO 2 NPs [90]. On the other hand, several authors observed that TiO 2 NPs in the dark did not exhibit toxic effects on bacteria [89,91].
Another possible interesting explanation for the intrinsic, non-photoinduced, antibacterial activity of TiO 2 , though non-nanostructured in nature, was reported by Lifen et al. who demonstrated that the germicidal activity of their sol-gel synthesized TiO 2 NPs was not UV-induced, under the investigated experimental conditions, being, instead, directly related to the wettability property of TiO 2 NPs [50]. The surface of TiO 2 NPs was found characterized by both hydrophobic and hydrophilic areas, the latter causing a deformation of the cells they get in contact with. In fact, the cell lost its rounded shape and flattened. This phenomenon was expected to build up pressure in the cell, which resulted in a burst of the cell and release of the contents.

Virus Inactivation
Viruses are ubiquitous biological entities much smaller than bacteria (0.01-0.03 µ). However, they are not independent organisms, presenting independent metabolic activities. Indeed, they need to infect a host for their reproduction. Viruses usually involved in waterborne disease outbreak are noroviruses (NoV), hepatitis A virus (HAV), hepatitis E virus (HEV), adenovirus (AdV), astrovirus, enteroviruses (EV), and rotavirus (RV). In particular, enteric viruses have high infectious capacity, that is, a low amount of viruses is sufficient to induce an infection (i.e., <10-10 3 virus particles) [92]. Viruses can often be detected on surfaces, because the structure of their capsid provides them environmental stability. Surfaces can play a crucial role in the spread of microorganisms, especially in a nosocomial environment, where the presence of viruses on the surfaces of equipment, furniture, and medical devices, as well as wall surfaces, represent a potential risk for patients and operators [93]. Moreover, viruses are also widespread in public spaces, including offices, canteens, and kindergartens. Once a surface is contaminated, it immediately becomes a contamination source for individuals and, consequently, other objects. For instance, in the case of NoV, it has been observed that it is possible to transmit the virus to up to seven different surfaces by simply handling contaminated objects [94]. Finally, viruses persist on the surfaces for an extremely long timeframe, ranging from 2-3 h (for HAV and coronavirus) to 20 weeks (for vaccinia virus) [95], thus being extremely critical for infection propagation.
When phage is used as a model, often the system involves the infection of a bacterium by a specific phage, such as the commonly investigated E. coli.
Generally, most experimental studies were carried out in aqueous solution, investigating virus exposure to TiO 2 NPs, both in the dark and under different irradiation conditions, given that the virus is able to interact with the TiO 2 NPs [96][97][98][99]102,108] both dispersed and immobilized on suitable substrate.
Many reports demonstrated how the environmental conditions can affect viral inhibition by photocatalytic TiO 2 NPs. For example, Syngouna et al. showed that the presence of quartz sand altered the antiviral efficiency of TiO 2 NPs against the bacteriophage MS2-E. coli both under sunlight irradiation and in the dark, resulting in a higher virus inactivation rate in absence of quartz sand. However, at higher virus concentration, the excessive viral density was demonstrated to inhibit the antiviral activity [97] of TiO 2 NPs. Zheng et al. obtained analogous results and assumed that a high viral density saturates the reactions sites on the photocatalyst surface, thus leading to limited ROS production [108]. Syngouna et al. (2017) also tested the antiviral activity of TiO 2 NPs in double distilled water (ddH 2 O) solution and in phosphate buffered saline solution (PBS), demonstrating that a higher MS2-E. coli inactivation could be obtained in ddH 2 O rather than PBS solution. This is most likely because the PBS induces MS2 aggregation, due to the ability of the proteins of MS2 to bind of phosphate ions, given that virus aggregation is known to reduce the efficiency of photocatalytic TiO 2 NPs in MS2 virus inactivation [97]. Different characteristics of the aqueous medium may affect the photocatalytic antiviral activity, including temperature [102], pH [98], and presence of inorganic ions [91]. The effect of temperature on the Cu-TiO 2 nanofiber-assisted removal of bacteriophage f2 under visible light irradiation was shown by Zheng et al. [108]. In particular, a relatively low temperature (15 • C) was found to negatively affect the virus removal rate, In fact, although in the first 30 min, the rate at 15 • C was the highest under experimental conditions, after 90 min it decreased significantly, resulting, instead, in the removal efficiency at 25 • and 35 • C being much higher. Koizumi et al. observed that inactivation rate of the phage MS2 in presence of TiO 2 P25 irradiated by black light fluorescent lamp was influenced by pH, given that it was higher at pH 6 than at pH 3.0 and 10.0 [98].
Moreover, the turbidity of water matrices, affecting the absorption of incident light [109], can reduce the photocatalytic efficiency, leading to a decrease of ROS production. Therefore, a suitable design of photocatalysis experiments needs to take into account the penetration depth of the wavelengths selected for the experiments [109]. For instance, in distilled water, 254 nm radiation loses 30% of its intensity already 40 cm below the solution surface. were investigated and both bacteriophages became inactivated when in contact with the TiO 2 -coated glass irradiated with 0.001 mW/cm 2 UV-A light. However, the inactivation of T4 was lower than that of Qβ when exposed to UV-A at an intensity lower than 0.01 mW/cm 2 , for 4 and 8 h. Although the photocatalysis was sufficient to inactivate both viruses, these results suggest that the ratio of inactivation varies according to the type of virus [100].
Gerrity et al. compared the disinfection potential of TiO 2 P25 NPs irradiated by a low-pressure UV-lamp with respect to a UV-light source, using bacteriophage PRD1, MS2, phi-X174 and fr, which are characterized by different physical and molecular features (size, nucleic acid composition and topology, genome length, mode of infection). The authors observed that in order to reach an inactivation rate of 4-log, a different UV intensity reduction, namely 19%, 15%, and 6%, was required for PRD1, MS2, and phi-X174, respectively. Interestingly fr was found to be UV-resistant [103]. The detected differences in inactivation efficiency were associated to structural differences among the investigated viruses.
Nakano et al. tested antiviral activity of TiO 2 coated glass on influenza virus (IFV) and feline calicivirus (FCV), according to the same procedure reported in Section 3.1.2. The authors found that IFV was inactivated by photocatalysts significantly faster than FCV, thus indicating that the virucidal effect of the photocatalyst may depend on the presence of a viral envelope, given that IFV is an enveloped virus, while FCV a non-enveloped virus [68].
The same authors studied the human influenza A virus as a viral model for the TiO 2 NPs-assisted photocatalysis Experiments [105], carried out by tuning irradiation time and UV-A lamp intensity in the range of 0.001-1.0 mW/cm. After 8 h incubation, a reduction of approximately 3-log was observed by using low intensity UV-A-light (0.01 mW/cm). A decrease of viral inactivation was observed with the decrease of UV-A light intensity. After 8 h incubation, a reduction of approximately 3-log was observed by using low intensity UV-A-light (0.01 mW/cm 2 ). However, even with an irradiation intensity of 0.001 mW/cm 2 , the TiO 2 -coated glass effectively inactivated the virus after 16 h of incubation, with a reduction of 4-log. At high UV-intensity (1.0 mW/cm 2 ), a much faster viral inactivation was found (3-log after 4 h). Therefore, irradiation intensity and time affect the inactivation efficiency of the prepared TiO 2 -coated glass against influenza virus.
The mechanisms of virus inactivation by TiO 2 NPs-assisted photocatalysis are a debated topic. Some authors suggest that ROS induce the capsid protein degradation, as demonstrated through different techniques (qPCR; sodium dodecyl sulphate-polycrylamide gel electrophoresis, SDS-page; etc) [97,100,103,110], while others (Nakano et al. (2012)) indicated, on the basis of real-time reverse-transcription PCR (RT-PCR) and SDS-page, RNA degradation only after the destruction of viral proteins involved in binding, therefore the infectability decreased [105]. Hajkova et al. demonstrated the photocatalytic effect of thin TiO 2 film in presence of UV-A light on the virus HSV-1. The experimental results proved that interaction of the virus with the photocatalytic surface caused significant changes in the virus structure, for instance, inducing the loss of viral glycoprotein, gC, responsible for the first attack of the virus on the target cell, thus resulting in the virus' inability to attack host cells [104].
The antigens, present in the capsid, were also considered to elucidate the effect of TiO 2 NPs. For example, the degradation of HBsAg, hepatitis B surface antigen, typical of hepatitis B virus (HBV) was evaluated in assessing its role in the photocatalytic antiviral activity of TiO 2 NPs. The degradation of the HbsAg antigen was found affected by the exposure time, amount of photocatalyst, and light-source. The inactivation of the antigen occurred after 12 h exposure. HbsAg degradation extent increased as the TiO 2 concentration increased. In addition, the effect of the light source on the photodestruction level of HbsAg was also shown, following the order UV lamp > mercury lamp > natural light > weak light (i.e., in the dark while not optically filtered) [110].
Furthermore, TiO 2 -NPs-based nanocomposites showed promising results for photocatalytic inactivation of viruses. A palladium-modified nitrogen-doped titanium oxide (TiON/PdO) photocatalytic fiber was effectively used for the disinfection of the coliphage MS2 with its host (E. coli) by Li et al. In the dark, a significant virus adsorption was measured (95.4-96.7%), while, after 1 h irradiation with visible light (λ > 400 nm) a virus removal of 94.5-98.2% was achieved [96]. Monmaturapoj et al. showed the effect of hydroxyapatite-titania composite (HAP/TiO 2 ) on H1N1, investigating the role played by nanocomposite amount, virus concentration, and UV-light irradiation time. An H1N1 photocatalytic inhibition efficiency dependent on the amount of photocatalyst was observed. In particular, no virus inhibition was measured for a HAP/TiO 2 concentration higher than 0.5 mg/mL. The HAP/TiO 2 nanocomposite was able to merge two functions in one nanomaterial: the virus adsorption on the photocatalyst surface, promoted by HAP, and ROS production, induced by UV light irradiation of TiO 2 [53]. Finally, SiO 2 -TiO 2 NPs and Ag-doped TiO 2 NPs (nAg/TiO 2 ) should be also mentioned for their high photocatalytic inactivation rates against the bacteriophage MS2 [49,111].
The current pandemic situation, related to the spreading of the SARS-CoV-2 virus, stimulated the researchers towards the understanding of its ability to persist on the surfaces [112], and towards the investigation of original and reliable solutions suitable for limiting the spread of SARS-CoV-2. Recently, Khaiboullina et al. (2020) explored the photocatalytic properties of nanosized TiO 2 NPs, deposited on glass coverslips, under UV radiation, against the inactivation of HCoV-NL63, namely a human coronavirus belonging to the family of α-coronaviruses, which also includes SARS-CoV-2 [113]. The experimental results highlighted the virucidal efficacy of photoactive TiO 2 NPs, as examined by quantitative RT-PCR and virus culture assays.

Fungi Inactivation
The scientific community has also started to intensively investigate antifungal properties of photocatalytic TiO 2 NPs. In spite of the low number of reports on the antifungal photocatalytic activity of TiO 2 nanostructured materials, increasing attention can be noticed, motivated by the ability of fungi (filamentous forms and yeasts) to produce dangerous mycotoxins in the water. Further, fungi and their spores are often present on surfaces, including foodstuff, as well as in indoor and outdoor environments. Fungi infect a host, behaving as parasites or by causing the decay of organic matter.
Compared to bacteria and viruses, fungi survive in much more stressful conditions (high saline/sugar concentration, high osmotic pressure and extreme pH). They have a complex structure and can show several morphologies. Indeed, molds are multicellular filaments, while yeasts are unicellular organisms. Fungi have a robust cell wall, mainly formed of chitin, with lamellar morphology, given that each layer is made up of fibrils crossing each other in different directions. Fungi (filamentous and yeast species, conidia, hyphae) showed different responses to the TiO 2 NPs treatment. Rodrigues-Silva et al. tested the effect, of TiO 2 -assisted photocatalysis on spores of A. fumigatus, both in water and air. After 60 min UV-A-irradiation, the inactivation of spores was observed, while after 180 min the complete inhibition of the microorganism's growth was detected. They pointed out a direct correlation between the TiO 2 NPs loading and the disinfection efficiency in aqueous media, as the higher the photocatalyst loading, the greater the photoinduced inactivation of fungi [82]. Seven et al. (2004) studied the effect of TiO 2 P25 (0.01 mg/mL) on different organisms (bacteria and fungi, such as filamentous and yeast species) using a sodium lamp as a light source. The TiO 2 disinfection ability was tested on different bacteria, in particular C. albicans and A. niger. C. albicans were inhibited after 120 min, whereas no inhibition was observed for A. niger even if the irradiation time was extended up to 240 min [114]. Furthermore, Polo-Lopez et al. (2010) tested TiO 2 P25 NPs on Fusarium sp and observed a greater resistance of the chlamydospores, followed by macroconidia and microconidia, due to their extremely complex structure, which is able to hinder the penetration of ROS [120]. The resistance to TiO 2 assisted deactivation of spores belonging C. albicans and A. niger was also reported [116,121,122]. Maneerat et al. (2006) tested the photocatalytic activity of TiO 2 powder and of a TiO 2 NPs-based coating on a plastic film, in vitro and on fruit rot (tomato and apple) in presence of UV-A source against P. expansum. [115] The severity of fruit rot was estimated by visual appearance and scored from 0 to 4 (0 = no decay; 4 = decay covering more than 50% of the whole fruit area). No inhibition effect on fungi growth was observed for the TiO 2 powder in the dark (control), while in presence of TiO 2 NPs under UV-A light irradiation, the growth decreased relative to the control experiment. Moreover, the TiO 2 NPs-based coating on the plastic film revealed P. expansum inhibition higher than in the control experiment (scores 1.9 vs. 3.2). Kuhn and co-workers (2003) investigated the damage induced by TiO 2 NPs under UV-A light irradiation by means of SEM analysis, highlighting a grainy and partially destroyed cell surface [123].
The differences among the various species of fungi in terms of structural features have been found reflected in their response to interaction with ROS generated by photocatalysis-assisted TiO 2 . The main difference among spores is in the wall as unicellular microconidia without septa and larger pluricellular macroconidia with septa both have a single cell wall, while chlamydospores exhibit a double thick wall [124]. Such a difference explains the highest resistance of chlamydospores (followed by macroconidia and microconidia) to photocatalysis [112].
Furthermore, the nature of the investigation medium also contributed to inactivation of spores in presence of TiO 2 and solar irradiation. Indeed inactivation rate values in distilled water were found higher than those detected in well water, most likely due to the presence of carbonates/bicarbonates [116]. Indeed, inorganic salt was reported to decrease the TiO 2 photocatalytic efficiency [125,126], mainly because of the formation of an inorganic layer at the TiO 2 surface.

General Considerations
In spite of the great attention attracted by the antimicrobial properties of TiO 2 nanostructured materials, the experimental evidence and the interpretation of the experimental results reported in the different studies, as also seen in this review, are often debated and, somehow, controversial.
Many of the discrepancies in the results can be ascribed to the diversity of methods used to investigate the photocatalytic disinfection. In order to highlight this variety of conditions, the experimental photocatalytic and operational parameters, including details of the set-up, for bacterial, viral, and fungal disinfection tests are collected in Tables 1-3, respectively. Furthermore, as summarised in Figure 8, TiO 2 NP characteristics ( Figure 8A) affect the photocatalytic efficiency while the microorganism structure ( Figure 8C), its metabolism ( Figure 8D), and local environment adopted for its growth ( Figure 8E) strongly affect the response of the microorganism to photocatalytic inactivation ( Figure 8B).  [62] x OD = optical density; BLB = black light blue lamps; PE = polyethylene; CFU = colony-forming units; t ma x = time at which the maximum inactivation rate (V max ) is achieved; log 10 (C/C 0 ) = the logarithm of reduction, where C0 is the concentration of control live bacteria without TiO 2 in the dark (CFU/mL), and C is the concentration of live bacteria for other conditions (CFU/mL). x PFU = plaque-forming unit; log 10 (C/C 0 ) = the logarithm of reduction, where C 0 is the concentration of control live bacteria without TiO 2 in the dark (CFU/mL), and C is the concentration of live bacteria for other conditions (CFU/mL); BLB = black light blue lamps; Q = microorganism removal efficiency; CFU = colony-forming units; HA = hydroxyapatite; TCID50 = fifty-percent-tissue-culture-infective-dose. x CFU = colony-forming units; BLB = black light blue lamp; score = visual appearance and scored from 0 to 4 (description in Section 3.3). Such considerations are intended to point out the relevance of a suitable experimental design, and the need to define reliable and reproducible analytical approaches to studying antimicrobial activity, which could, possibly, also be standardized in order to provide comparable data. In Figure 9, common methods for testing the microorganism inactivation ability of a specific system are outlined. Such considerations are intended to point out the relevance of a suitable experimental design, and the need to define reliable and reproducible analytical approaches to studying antimicrobial activity, which could, possibly, also be standardized in order to provide comparable data. In Figure 9, common methods for testing the microorganism inactivation ability of a specific system are outlined. Commonly, antibacterial activity is investigated using the disk diffusion method. According to this approach, NPs are incorporated in a solid matrix or impregnate a paper filter placed on an agar plate (Kirby Bauer agar plating technique). At this stage, bacteria are allowed to grow on the agar plate and the evaluation of the diameter of the inhibitory zones allows to determine the efficiency of bacterial growth inhibition [25,33,134]. In the disk diffusion method, a high number of experimental parameters (different dimensions of spot diameter; cell density used; amount of catalyst) is present, that can, in principle, result in a misled interpretation of the results, thus leading to contradictory conclusions as a function of the different parameters. Although the disk diffusion method is simple and widely used for testing the antibacterial efficiency, it shows some limitations, such as the limited diffusion of the material to be tested in the agar matrix, a poorly resolved concentration gradient around the disks, and the absence of a suitable light source that makes this tool not ideal for the photocatalytic experiments, which require an irradiation source. Therefore, to specifically study the TiO2-based NPs-assisted photocatalytic inactivation of microorganisms, dedicated experimental set-ups have been proposed, including a proper light source with specifications suitable for the investigation to be carried out [37,61,127,135].
Another issue is caused by the need to evaluate viability or condition of bacteria. For this purpose, a viable count method or the live/dead cell viability assay can be used. The former test allows to simultaneously control the viability of bacteria (live and viable, but not culturable cells), while the latter method allows the simultaneous determination of live and dead cells using two fluorescence dyes: ethidium homodimer III (EthD-III) and calcein acetoxymethyl (calcein AM).
Therefore, the method used to investigate the antimicrobial activity could strongly affect the reliability and interpretation of the experimental results.  Commonly, antibacterial activity is investigated using the disk diffusion method. According to this approach, NPs are incorporated in a solid matrix or impregnate a paper filter placed on an agar plate (Kirby Bauer agar plating technique). At this stage, bacteria are allowed to grow on the agar plate and the evaluation of the diameter of the inhibitory zones allows to determine the efficiency of bacterial growth inhibition [25,33,134]. In the disk diffusion method, a high number of experimental parameters (different dimensions of spot diameter; cell density used; amount of catalyst) is present, that can, in principle, result in a misled interpretation of the results, thus leading to contradictory conclusions as a function of the different parameters. Although the disk diffusion method is simple and widely used for testing the antibacterial efficiency, it shows some limitations, such as the limited diffusion of the material to be tested in the agar matrix, a poorly resolved concentration gradient around the disks, and the absence of a suitable light source that makes this tool not ideal for the photocatalytic experiments, which require an irradiation source. Therefore, to specifically study the TiO 2 -based NPs-assisted photocatalytic inactivation of microorganisms, dedicated experimental set-ups have been proposed, including a proper light source with specifications suitable for the investigation to be carried out [37,61,127,135].
Another issue is caused by the need to evaluate viability or condition of bacteria. For this purpose, a viable count method or the live/dead cell viability assay can be used. The former test allows to simultaneously control the viability of bacteria (live and viable, but not culturable cells), while the latter method allows the simultaneous determination of live and dead cells using two fluorescence dyes: ethidium homodimer III (EthD-III) and calcein acetoxymethyl (calcein AM). Therefore, the method used to investigate the antimicrobial activity could strongly affect the reliability and interpretation of the experimental results.
Another important issue represented by the need for a standardization of test method is the selection of the microorganism to be used as a target for photocatalytic antimicrobial experiments, especially for a hypothetical future application of the material studied (water-, air-, and food-borne). Beyond the structural properties (i.e., Gram-positive vs. Gram-negative bacteria), the final applications field of the NPs studied should also be considered. Therefore, it is also important to consider the correct indicator microorganism. It follows that the target microorganism can also be selected as a function of the specific application the investigated TiO 2 NPs-based nanocomposite is designed for. In other words, E. coli, Shigella flexneri (S. flexneri), L. monocytogenes, and Vibrio parahaemolyticus (V. parahaemolyticus) are indicators of contaminated waters. They can therefore be selected as target microorganisms for TiO 2 NPs-based nanocomposites specifically designed for water remediation, while indicators of nosocomial infections such as Streptococcus pyogenes (Str. pyogenes), Acinetobacter baumannii (Acin. baumanii), St. aureus, and P. aeruginosa can be used as target microorganisms to investigate TiO 2 NPs-based nanocomposites designed to be used in healthcare facilities.
Taking into account the above reported considerations, it is apparent that the lack of standardized techniques suitable for evaluating the photocatalytic antimicrobial activity of TiO 2 NPs underlining the different actions on the microbial inhibition is at present a strong limitation in unequivocally assessing the photocatalytic antimicrobial properties of TiO 2 .
Furthermore, for fungi, the proposed methods to test the antifungal activity may vary significantly in the different reports. Some authors investigate antifungal activity in a fungal suspension containing the TiO 2 -based powder irradiating with a light source that can be very different in term of light flux and emission wavelength [82,114,116,117,123]. The photocatalytic activity is evaluated by examining the growth of the investigated fungus by plate count relative to an untreated control sample. As an alternative, the photocatalysts can be applied directly to fruit or vegetable surfaces (e.g., apple, tomato, or lemon) [115,117] and the photocatalytic activity can be evaluated by simply measuring the diameter of the fungal colonies grown with relative to an untreated control sample.
In conclusion, photocatalytically active TiO 2 NPs show great potential for antiviral and antifungal related applications. However, while antiviral activity is well documented, the photocatalysis-promoted antifungal activity of TiO 2 NPs, still deserves more investigation, considering, in particular, the role played by the structural features of the microorganisms in terms of thickness of spores and cell wall.

Technological Applications of Antimicrobial TiO 2 -Based Nanostructured Materials
In this section, selected applications of the antimicrobial TiO 2 -based materials will be described focusing the attention on environmental applications, including water treatment, anti-biofouling membranes for water treatment, disinfection of building materials, and disinfection of (i) biomaterial and (ii) food packaging and processing materials.

TiO 2 -Based Nanostructured Materials for Water Disinfection
TiO 2 based nanocomposites are well known and extensively used materials in water treatment technologies [3,4,136]. Indeed they are able, due to their photocatalytic activity, to degrade organic pollutants such as dyes, [137] pesticides [138], pharmaceuticals [3,139], and personal care products [140], that pose a great danger for human and aquatic life. Moreover, their antimicrobial properties are effective against waterborne pathogens such as bacteria, viruses, and fungi that originate from the urban microbiome and tend to be released and accumulated in sewage and urban runoff. The mechanism is summarized in Figure 10 and will be explained in the following. Waterborne diseases derive often from bacteria such as E. coli, Legionella pneumophila, Mycobacterium avium, S. flexneri, L. monocytogenes, V. parahaemolyticus and, to a lesser extent, from viruses, that are typically present at lower concentration.
Every year, waterborne infections cause nearly 200 million deaths worldwide, mainly localized in low income countries [129]. One of the most critical classes of bacteria in wastewater treatment plants (WWTPs) is antibiotic resistant bacteria (ARB), which derive from the extensive use and abuse of antibiotics. WWTPs offer an ideal environment for ARB proliferation and, under these conditions, the antibiotic resistance genes (ARG) that induce the resistance in the bacterial population, can spread easily and quickly. Therefore, as the use of common antibiotics becomes ineffective, it is fundamental to develop alternative strategies to treat microbial infections and to promptly take action against the spreading of ARB in water as well as in the air [141]. The present section will focus on TiO 2 -based nanomaterials as an excellent alternative to conventional methods for water disinfection. In addition, nanomaterials applied to counteract the biofouling will be reviewed, as it is another great problem affecting water treatment plants as well as materials in contact with water, including distribution pipes and filtration membranes [142].
Catalysts 2020, 10, x 26 of 43 lower concentration. Every year, waterborne infections cause nearly 200 million deaths worldwide, mainly localized in low income countries [129]. One of the most critical classes of bacteria in wastewater treatment plants (WWTPs) is antibiotic resistant bacteria (ARB), which derive from the extensive use and abuse of antibiotics. WWTPs offer an ideal environment for ARB proliferation and, under these conditions, the antibiotic resistance genes (ARG) that induce the resistance in the bacterial population, can spread easily and quickly. Therefore, as the use of common antibiotics becomes ineffective, it is fundamental to develop alternative strategies to treat microbial infections and to promptly take action against the spreading of ARB in water as well as in the air [141]. The present section will focus on TiO2-based nanomaterials as an excellent alternative to conventional methods for water disinfection. In addition, nanomaterials applied to counteract the biofouling will be reviewed, as it is another great problem affecting water treatment plants as well as materials in contact with water, including distribution pipes and filtration membranes [142]. Figure 10. General scheme of TiO2 NPs-based nanocomposite application for water disinfection, that highlights the ability to prevent biofilm formation at the surface of the substrate, such as a membrane, and the antibacterial activity against water pathogens driven by photocatalysis.
TiO2 NPs-based nanocomposites for water disinfection are often investigated as colloidal suspensions, since, under these conditions, the whole NPs surface is available, exposing all the active catalytic sites to the aqueous environment dispersing the pathogens. However, while suspended NPs are typically found to display a higher photocatalytic activity in comparison with NPs immobilized on a substrate [143], the dispersed NPs present technological problems, as they need to be recovered to prevent them from becoming a source of pollution in the environment.
Biancullo et al. investigated the effect of TiO2 NPs on the inactivation of ARBs and antibiotics in urban wastewater and showed that the photocatalytic treatment inactivated E. coli and Enterococci sp. However, bacteria regrowth experiments demonstrated that the bacteria population over time was not inhibited and that the ratio between resistant and non-resistant cells, detected before and after the TiO2-assisted photocatalysis treatment, stayed unchanged [144]. Venieri et al. proposed metaldoped TiO2 (Mn-, Co-, and binary Mn/Co-doping) nanomaterial against K. pneumoniae in real wastewater. After 90 min of exposure to artificial sunlight, a decrease in bacterial population, in the range from 4 to 6 logs cycles, was measured in each investigated case and the co-doped material (0.04% wt) was found to be effective like the single doped (0.1% wt) samples. Nevertheless, the Figure 10. General scheme of TiO 2 NPs-based nanocomposite application for water disinfection, that highlights the ability to prevent biofilm formation at the surface of the substrate, such as a membrane, and the antibacterial activity against water pathogens driven by photocatalysis. TiO 2 NPs-based nanocomposites for water disinfection are often investigated as colloidal suspensions, since, under these conditions, the whole NPs surface is available, exposing all the active catalytic sites to the aqueous environment dispersing the pathogens. However, while suspended NPs are typically found to display a higher photocatalytic activity in comparison with NPs immobilized on a substrate [143], the dispersed NPs present technological problems, as they need to be recovered to prevent them from becoming a source of pollution in the environment.
Biancullo et al. investigated the effect of TiO 2 NPs on the inactivation of ARBs and antibiotics in urban wastewater and showed that the photocatalytic treatment inactivated E. coli and Enterococci sp. However, bacteria regrowth experiments demonstrated that the bacteria population over time was not inhibited and that the ratio between resistant and non-resistant cells, detected before and after the TiO 2 -assisted photocatalysis treatment, stayed unchanged [144]. Venieri et al. proposed metal-doped TiO 2 (Mn-, Co-, and binary Mn/Co-doping) nanomaterial against K. pneumoniae in real wastewater. After 90 min of exposure to artificial sunlight, a decrease in bacterial population, in the range from 4 to 6 logs cycles, was measured in each investigated case and the co-doped material (0.04% wt) was found to be effective like the single doped (0.1% wt) samples. Nevertheless, the experiment performed under natural sunlight irradiation showed a lower photocatalytic efficiency, as a 2-log bacteria population reduction was detected in presence of the Mn/Co-TiO 2 nanocomposite. Moreover, the resistance of the bacteria surviving cells to some antibiotics was found to be decreased even though ARGs were still detectable, and thus found to be potentially able to develop antibiotic resistance [39]. Rizzo et al. reported the antibacterial activity of N-doped TiO 2 NPs against the antibiotic resistant E. coli from urban wastewater. They found a higher activity of N-doped TiO 2 NPs compared to the commercial benchmark TiO 2 P25 and identified 0.2 g/L NPs concentration suited to obtain, under simulated sunlight irradiation, the highest inactivation rate (8.5 × 10 5 CFU 100 mL −1 min −1 ) after 10 min of irradiation and total inactivation after 60 min. No significant change in E. coli resistance to selected antibiotics (ciprofloxacin, cefuroxime, tetracycline, and vancomycin) was observed after treatment [145]. The same authors investigated the effect of TiO 2 P25 on the same bacteria as a function of irradiation type, testing four different light sources: a wide spectrum 250 W lamp, the same lamp equipped with a filter to simulate solar radiation, a 125 W black light fluorescent lamp, and real solar light. A higher efficiency was found when artificial solar light was used to irradiate for 60 min 0.05 g/L of TiO 2 suspension with respect to solar light. Differences in antibiotic resistance were detected as a function of the irradiation source. On the other hand, the unfiltered 250 W lamp was able to inactivate E. coli without any catalysts, whereas the 125W black light fluorescent lamp catalyzed a 70% inactivation of E. coli in the presence of 0.1 g/L of TiO 2 [146]. Ghosh et al. described an antibacterial activity, under UV-vis irradiation of a Ag-TiO 2 nanocomposite, obtained upon ball-milling of Ag and P25, against E. coli and St. aureus higher than that of the bare TiO 2 NPs. A similarly high photocatalytic efficiency was observed for the degradation of organic molecules. The nanocomposite was found not to be cytotoxic below a 50 µg/mL concentration [49]. Liu et al. proposed a vertical face-to-face heterojunction obtained by assembling horizontally TiO 2 nanosheets, exposing 001 facet, with graphitic carbon nitride (g-C 3 N 4 ) sheets, obtaining a Z-scheme electron transfer that enhances the charge separation and therefore the photocatalytic and antibacterial activity. In particular, a 96.8% reduction of E. coli was achieved after 30 min of simulated solar light irradiation, against a 50% reduction obtained with unmodified TiO 2 . In the dark, the nanocomposite promoted only a 5% reduction of the E. coli population after 30 min, thus confirming that the bacteria inactivation occurred mainly due to photocatalytic ROS production [45]. Liga et al. evaluated the biocide activity, both at dark and under UV-A irradiation, of Ag-TiO 2 obtained by photoreduction of AgNO 3 , against bacteriophage MS2, a model virus for common waterborne pathogens, characterized by a high resistance to common water disinfection methods. The inactivation activity of this system under UV was up to five times higher than that observed for commercial TiO 2 P25 and was found to enhance with the increase of the Ag amount [49].
Kim et al. reported on a nanocomposite based on TiO 2 NPs coupled with glucose oxidase (GOx), an enzyme acting as organic biocatalyst in presence of glucose, purposely selected as a high concentration of glucose favours the proliferation of heterotrophic bacteria, thus providing a (photo)catalytic system able to decrease the amount of available glucose and, concomitantly, to increase the production of reactive species, responsible for bacteria inactivation. Under UV irradiation with a 4W UV lamp (λ max = 352 nm), inactivation of E. coli assisted by TiO 2 -GOx was higher than that assisted by bare TiO 2 , and such enhancement was much more relevant in the presence of glucose [52].

Immobilization of Nanocomposites on Membranes or Recoverable Supports
The immobilization of photocatalytic TiO 2 NPs and their nanocomposites onto a suitable substrate is essential to accomplish technologically feasible applications. As mentioned above, a robust and durable immobilization of NPs onto appropriate support is fundamental, not only for enabling the nanosized photocatalyst recovery, but also to prevent the accidental release of NPs in the environment, that, becoming themselves a possible contaminant, may become harmful [4]. Here, some of the most original proposed solutions for the deposition and immobilization of TiO 2 NPs-based nanomaterials specifically designed for photocatalytic microorganism inactivation are summarized.
Li et al. developed a foam based on photocatalytic TiO 2 NPs; a promptly separable system, and, as it floats at water surface, it is able to efficiently exploit sunlight for photocatalytic generation of ROS [147]. Another ingenious solution is based on nanocomposites also incorporating a magnetic domain, able to convey to the system magnetic properties effective in enabling their simple recovery at the end of the treatment by applying a magnetic field. Wu et al. synthetized a ternary composite based on lanthanum-doped TiO 2 /calcium ferrite/diatomite with magnetic properties, and enhanced photocatalytic antibacterial activity against E. coli [46]. Another interesting alternative to achieve recoverable photoactive materials is given by nanocomposites formed of NPs coupled with micrometer structures, thus combining the advantages of the nanoscale properties with the possibility of recovering the composite by using filtration methods. Indeed, Xiao et al. reported an original multifunctional system, based on chitosan (CS) beads decorated with TiO 2 nanostructures modified with Ag NPs, able to effectively merge the advantage of a recoverable and reusable material and an enhanced antibacterial activity, synergistically accomplished by each of the three components of the nanocomposite, as effectively demonstrated against E. coli upon UV-A irradiation [50].
Negishi et al. used a similar approach with commercial TiO 2 coated silica gel beads (4 mm diameter). The coated beads were applied in a solar water purification system for disinfection of tap water from a village in Thailand. A reduction of coliform and general bacteria was observed during three days, together with a degradation, after long time exposure, of the silica component of the bead, resulting in loss of the TiO 2 layer. [148] However, the most extensively explored immobilization approach relies on the incorporation of the photocatalyst in polymeric materials [149]. In particular, polymeric membranes have been thoroughly applied in water treatment technologies as microfiltration, ultrafiltration, nanofiltration, membrane bioreactors, and membrane with biocide activity for water disinfection, through the integration of TiO 2 nanostructured material [150]. Haghighat et al. designed a nanocomposite Ag/TiO 2 /polyvinyl chloride (PVC) membrane for ultrafiltration (UF) by incorporating well dispersed Ag/TiO 2 NPs in the host polymer. Such a nanocomposite-based membrane displayed bacterial inactivation ability against P. aeruginosa, E. coli, and St. aureus, together with antifouling properties and degradation activity against an organic dye [51]. Li et al. reported a nanofiltration (NF) membrane obtained by coating a polyethersulfone (PES) substrate with a film of nanocomposite consisting of TiO 2 NPs (functionalized with tannic acid) embedded in polyester. In particular, the membrane prepared with 0.020 wt% TiO 2 tannic acid (TA) solution showed an E. coli population reduction of 77.2% under dark conditions and a 99.2% E. coli population reduction after UV irradiation at 365 nm [54].
Photocatalytic membrane reactors (PMRs) combine the membrane technology with the advantages of photocatalysis assisted reactions. Two main configurations are reported in the literature: PMRs with the photocatalyst immobilized on the membrane or incorporated therein, and PMRs based on the photocatalyst dispersed in a colloidal suspension. In both cases the photocatalyst is confined in the reaction environment. However, the former configuration allows an effective recovery and reuse of the catalyst, thanks to its stable immobilization, while the recovery is more complicated in the latter case, although the whole NPs surface can be more effectively exploited for adsorption and catalysis [151]. Among the possible photocatalysts for PMR fabrication, TiO 2 NPs and TiO 2 NPs-based nanocomposites have been the most investigated [152]. Cheng et al. fabricated a PMR based on the combination of a polyvinylidene fluoride (PVDF) membrane and a suspension of TiO 2 P25 in tap water and studied the biocide effect for virus removal, using phage f2 as a model. The effect of the amount of humic acid (HA) in the water dispersion on the efficiency of the system was also investigated. Moreover, HA was found to compete with phage f2 at the adsorption sites on TiO 2 NPs surface, and the UV light absorbed by HA overall led to a reduction of biocide activity at high HA concentration [153]. A PMR based on a microfiltration hollow polyethylene (PE) fiber membrane coupled with a suspension of TiO 2 P25 was investigated for the reduction in bacterial population of secondary effluent water sample, that was assessed, down to 2 log, when a 1g/L TiO 2 NPs suspension was used [154]. Another typical and efficient approach for nanocatalyst immobilization, able to preserve their activity, relies on their immobilization onto fibers that, possessing a high surface, allow optimal interaction between the target substrate dispersed in water and the photocatalyst. Amarjargal et al. functionalized the surface of electrospun polyurethane (PU) fibers with Ag-TiO 2 NPs by simply dipping the fiber in hot colloidal photocatalyst dispersion for 4 h and 12 h. After 3 h UV irradiation (320-500nm), a 6-log cycles reduction of E. coli population was found for the sample obtained after 4 h immersion and a 5-log cycles reduction for that immersed for 12 h. Interestingly, only 2-log cycles reduction was detected after exposure to UV, in absence of Ag-TiO 2 and no reduction was observed upon exposure of the nanocomposite to ambient light [35]. The antibacterial activity of electrospun Ag-TiO 2 nanofibers was tested against E. coli. The study demonstrated that, under the investigated experimental conditions: (i) no biocidal activity was obtained upon exposure to light (solar simulator) of the bare fiber, nor in the dark for TiO 2 /Ag fibers and P25-coated fibers, (ii) fibers functionalized with the synthesized TiO 2 performed better, in terms of photocatalytic antibacterial activity, than fibers coated with TiO 2 P25, and (iii) the antibacterial activity in presence of Ag NPs was found higher in the dark than under light irradiation [155]. A TiO 2 /Polyamide 6 (PA-6) electrospun fiber was tested by Daels et al. for the removal of St. aureus and HA from a secondary effluent sample. HA reduction of 83% after 2 h irradiation with simulated solar light (300 W Osram Ultra-Vitalux lamp) with an intensity of about 5 mW/cm 2 and 99.99% bacteria reduction after 6h UV irradiation was detected. The same fibers, tested in a filtration process (flow rate 11 m 3 /(m 2 h)) in a photocatalytic experiment performed under UV irradiation achieved 37% HA reduction and 76% St. aureus inactivation [18]. Zheng et al. electrospun Cu-TiO 2 nanofibers and tested the biocide activity under visible light against f2 phage and the system formed by f2 phage and E. coli as its host, and reported a photocatalytic inactivation of E. coli higher than that of f2 phage. Remarkably, the E. coli inactivation did not seem affected by the presence of f2 phage, while the inactivation of the phage f2 in the bacteria host system was lower than that found in absence of E. coli. Such a result was explained by considering that E. coli can behave as a ROS scavenger, probably due to its membrane structure, as discussed above, thus reducing amount of ROS effective for f2 inactivation, thus somehow preventing its inactivation [108]. Ishikawa et al. proposed an interesting approach to obtain ceramic fibers characterized by an outer TiO 2 NPs enriched layer. Namely polycarbosilane, -SiH(CH 3 )-CH 2 -) n , was mixed with Ti(OC 4 H 9 ) 4 (50 wt%) in order to obtain a fiber by melt spinning, that was then exposed to air at 70 • C for 100 h. The resulting fiber was composed of amorphous SiO 2 and nanometric TiO 2 in anatase phase that, for a bleed-out process, migrated to the surface of the fiber. The functionalized fiber demonstrated an ability to completely remove coliform from wastewater after 3 h UV irradiation (352 nm, 2 mW/cm 2 ) [156].

Anti-Biofouling Membranes for Water Treatment
The biofouling process is defined as the accumulation of microorganisms on a surface, and is realized in four steps: (i) organic matters are adsorbed on the surface, (ii) microorganisms adsorb and adhere to the surface, and (iii) start to grow and reproduce on the surface, and finally (iv) extracellular polymers are secreted and mutual adhesion among the clonal cells takes place [157]. These processes ultimately result in the formation of a biofilm, namely an assembly of microbial cells, irreversibly bound to a surface and embedded in a matrix of polysaccharide material [158]. When biofouling takes place at the surface of a membrane, its flux is limited, thus interfering with rejection and reducing the lifetime of the membrane itself, with the consequent drawbacks. In order to prevent biofilm formation, two main strategies can be employed. The first relies on the ability to enhance the hydrophilicity of the membrane, which reduces the affinity of the organic matter, and enhances its affinity with water, that can, thus, form a thin layer on the membrane surface, finally hampering the microorganisms' adhesion. The second route is based on the incorporation of antibacterial agents into the membrane, such as TiO 2 -based nanomaterials [51]. TiO 2 -based nanocomposites are greatly suggested for this application since they display antibacterial properties and, concomitantly, convey, as a function of their chemical nature, hydrophilicity to the host membrane [159]. The combination of the intrinsic antibacterial properties with the photocatalytic properties of TiO 2 NPs-based nanocomposites offers multiple advantages for hindering the biofouling. As an example, Kim et al. deposited TiO 2 NPs on a polyamide thin film composite (TFC) membrane and tested their anti-biofouling properties, along with the antibacterial activity against E. coli. The water flux was measured for three days, for pristine and TiO 2 NPs-modified membrane, and with and without UV-light irradiation. After pipetting E. coli dispersion onto the membranes and incubating at 37 • C, a fast and significant reduction of the flux, a clear indication of the occurrence of a higher fouling, was observed for the membranes not exposed to UV, while a slow and low reduction of the flux was found for TiO 2 -modified membranes, especially upon UV irradiation, finally demonstrating how the anti-biofouling activity is tightly connected to antibacterial behavior of UV-active TiO 2 photocatalysts [19].

TiO 2 NPs-Based Nanocomposite against Biofouling on Building Materials
In the construction field, nanostructured TiO 2 is commonly employed to degrade organic pollutants in the air or to protect the building materials from soot, due to its photocatalytic and hydrophobic properties. The biocide activity of TiO 2 can also be exploited to reduce the formation of biofilms formed on the surface of buildings [160]. Indeed, biofouling causes, not only aesthetic and structural degradation of construction materials and surfaces, but also bacteria and fungi proliferation that may pose a great health concern [149]. The application of photocatalytic TiO 2 NPs as a biocidal agent has considerable relevance, especially for locations strongly sensitive to biological safety, such as medical facilities and food industries, where usually ceramic tiles are used to coat walls and floor. The photocatalytic activity of TiO 2 nanomaterials was found to successfully reduce the bacterial proliferation and the fungi population (and, consequently, mycotoxins production) indoor when applied to surfaces, tiles, furniture etc. [161]. Dyshlyuk et al. tested suspensions of TiO 2 , ZnO, and SiO 2 against microorganisms known for damaging construction materials, namely a bacterium (B. subtilis) and several common fungi (A. niger, Aspergillus terreus, Aureobasidium pullulans var. pullulans, Cladosporium cladosporioides, Penicillium ochrochloron, Trichoderma viride, and Paecilomyces variotii). They found TiO 2 and SiO 2 less active under sunlight than ZnO [162]. Sikora et al. fabricated core-shell nanocomposites of mesoporous silica (mSiO 2 , core) and TiO 2 (shell) to be applied on cement mortars. The merging of the two nanomaterials in one nanocomposite was found to play the two roles of introducing in the building material a filler, mSiO 2 , able to improve its mechanical properties, and conveying, with TiO 2 , anti-biofouling and self-cleaning activity upon UV exposure. The mSiO 2 /TiO 2 composite antibacterial activity was tested against E. coli and, after 2 h, a 67% and a 42% reduction were measured after exposure to UV light and at dark, respectively [46].

Photocatalytic TiO 2 NPs-Based Nanocomposites for Biomaterials Disinfection
Antibacterial NPs find a wide range of applications in the field of biomaterials. Materials designed for wound dressing or for tissue engineering-related applications need to comply with several requirements as summarized in Figure 11, as they need to be biocompatible, non-allergenic, easily removable, and degradable after implantation. Figure 11. General scheme of TiO2 NPs-based nanocomposite application for biomaterial-related applications highlighting the ability to repel water, to adhere to the skin following its movements, to remove exudates, to be permeable to air, and to prevent infections due to the antimicrobial activity driven by photocatalysis.
Antibacterial behavior is strongly desirable in this kind of material to prevent infections during wound recovery [163], [164]. A great deal of work has been carried out in the development of biocompatible TiO2-NPs-based nanocomposites, with intrinsic antimicrobial activity, specifically designed for tissue engineering-related applications to be used in healthcare facilities [33,[165][166][167][168][169].
In this scenario, several research groups have explored the advantages of TiO2-based nanocomposites considering both their biocompatibility and the intrinsic antibacterial properties, focusing also on their excellent photocatalytic properties that can be extremely advantageous in specific biomedical applications. Monmaturapoj et al. fabricated a nanocomposite made of TiO2 and HAP NPs for face masks serving as filtration devices. HAP is a type of calcium phosphate-based ceramic material widely used in the biomaterial field, especially in orthopaedic implants, being biocompatible and a very good substitute for bone. After assessing the antibacterial activity of the nanocomposite, [170] strong antiviral activity against H1N1 influenza A virus was observed under UV-light irradiation, with a synergic effect of HA and TiO2 NPs that revealed optimal for HA/TiO2 (50:50) [53]. Li et al. coated a face mask for medical purposes with NPs formed of a mixture of silver nitrate and TiO2 NPs in order to work as photocatalyst, reduce the bacteria population and to inhibit their growth on the surface, due to the presence of the exhaled moisture from breathing. A 100% reduction of bacteria on the surface of the coated mask 24 h after E. coli and St. aureus inoculation was found against a 25% and 50% increase measured on the uncoated masks for the two bacteria, respectively [171].

TiO2 NPs-Based Nanocomposites Designed for Disinfection of Food Packaging and Processing Materials
Food packaging materials containing TiO2 NPs-based nanocomposites have been thoroughly investigated materials for food contact-related applications and a steadily increasing number of technologies has been in development in the last years. This is in order to accomplish multiple purposes including improved mechanical, thermal, optical and antimicrobial properties, control of gas and moisture permeability, UV shielding, nutraceuticals release, and installing sensors for pathogens and harmful substances ( Figure 12) [172][173][174]. Indeed, surfaces of food-processing plant components need to comply with the same requirements, as they are often in contact with food and the presence of microorganisms therein can easily lead to food contamination, causing transmission of diseases [153]. Indeed, the presence of antimicrobial substances in food packaging materials may Figure 11. General scheme of TiO 2 NPs-based nanocomposite application for biomaterial-related applications highlighting the ability to repel water, to adhere to the skin following its movements, to remove exudates, to be permeable to air, and to prevent infections due to the antimicrobial activity driven by photocatalysis.
Antibacterial behavior is strongly desirable in this kind of material to prevent infections during wound recovery [163,164]. A great deal of work has been carried out in the development of biocompatible TiO 2 -NPs-based nanocomposites, with intrinsic antimicrobial activity, specifically designed for tissue engineering-related applications to be used in healthcare facilities [33,[165][166][167][168][169].
In this scenario, several research groups have explored the advantages of TiO 2 -based nanocomposites considering both their biocompatibility and the intrinsic antibacterial properties, focusing also on their excellent photocatalytic properties that can be extremely advantageous in specific biomedical applications. Monmaturapoj et al. fabricated a nanocomposite made of TiO 2 and HAP NPs for face masks serving as filtration devices. HAP is a type of calcium phosphate-based ceramic material widely used in the biomaterial field, especially in orthopaedic implants, being biocompatible and a very good substitute for bone. After assessing the antibacterial activity of the nanocomposite, [170] strong antiviral activity against H1N1 influenza A virus was observed under UV-light irradiation, with a synergic effect of HA and TiO 2 NPs that revealed optimal for HA/TiO 2 (50:50) [53]. Li et al. coated a face mask for medical purposes with NPs formed of a mixture of silver nitrate and TiO 2 NPs in order to work as photocatalyst, reduce the bacteria population and to inhibit their growth on the surface, due to the presence of the exhaled moisture from breathing. A 100% reduction of bacteria on the surface of the coated mask 24 h after E. coli and St. aureus inoculation was found against a 25% and 50% increase measured on the uncoated masks for the two bacteria, respectively [171].

TiO 2 NPs-Based Nanocomposites Designed for Disinfection of Food Packaging and Processing Materials
Food packaging materials containing TiO 2 NPs-based nanocomposites have been thoroughly investigated materials for food contact-related applications and a steadily increasing number of technologies has been in development in the last years. This is in order to accomplish multiple purposes including improved mechanical, thermal, optical and antimicrobial properties, control of gas and moisture permeability, UV shielding, nutraceuticals release, and installing sensors for pathogens and harmful substances ( Figure 12) [172][173][174]. Indeed, surfaces of food-processing plant components need to comply with the same requirements, as they are often in contact with food and the presence of microorganisms therein can easily lead to food contamination, causing transmission of diseases [153]. Indeed, the presence of antimicrobial substances in food packaging materials may inhibit growth of harmful microorganisms and pathogens on food, improving its safety and shelf life. NPs and nanocomposites can be integrated in food packaging materials as growth inhibitors, antimicrobial agents, or carriers [175], and are usually applied to the packaging of meat, fish, poultry, bread, cheese, fruits, and vegetables [176]. TiO 2 NPs, aside from antibacterial properties, also possess unique characteristics that may prove particularly useful for such applications. For example, TiO 2 shields UV light, a property that is generally exploited in the formulation of sunscreen lotions, and therefore may be an effective additive in packaging to preserve food from irradiation, and thus limit its deterioration [177,178]. Moreover, when TiO 2 NPs are contained in a packaging material, upon UV exposure they can photocatalytically degrade the ethylene molecules produced during the ripening process, which are responsible for food degradation [179].
unique characteristics that may prove particularly useful for such applications. For example, TiO2 shields UV light, a property that is generally exploited in the formulation of sunscreen lotions, and therefore may be an effective additive in packaging to preserve food from irradiation, and thus limit its deterioration [177,178]. Moreover, when TiO2 NPs are contained in a packaging material, upon UV exposure they can photocatalytically degrade the ethylene molecules produced during the ripening process, which are responsible for food degradation [179].
In the last years, the choice of a polymer suitable and environmentally sustainable for food packaging has oriented towards biopolymers, which can be easily degraded and recycled, for a more ecological fingerprint in a field that has been, so far, heavily relying on petroleum-based polymers. Such biopolymers include poly hydroxybutyrates (PHB), polylactic acid (PLA), poly caprolactone (PCL), polyvinyl alcohol (PVA), poly butylene succinate, lipids (wax and free fatty acids), proteins (casein, whey, and gluten), polysaccharides (starch and cellulose derivatives, alginates, and chitosan) and their possible blends [180]. In particular, chitosan, a polysaccharide derived from chitin, is especially suited for food packaging, considering, as reported above, its biocompatibility, biodegradability, and antibacterial and antifungal behavior, along with good film forming properties [177,181]. Zhang et al. developed a coating for food packaging based on TiO2 NPs embedded in a chitosan (CS) matrix. The presence of TiO2 NPs in a chitosan film served to improve, upon exposure to visible light, its mechanical properties, wettability and antibacterial activity against typical food pathogens (E. coli, St. aureus, C. albicans, and A. niger) with respect to film formed by bare chitosan. The effect of the direct application of the CS/TiO2 coating on red grapes was also tested and was shown to preserve the fruit better than conventional plastic coating [181]. Li et al. reported a composite film where Ag-TiO2 nanocomposites where embedded in a mixture of fish gelatin (FG) and CS, given that FG is a protein with good film forming, and antioxidant and water locking properties. The addition of CS imparted to FG enhanced mechanical properties while the integration of the Ag-TiO2 nanocomposite, Figure 12. General scheme of TiO 2 NPs-based nanocomposite application for food packaging highlighting the advantages of TiO 2 in the coating as improved mechanical properties, water and oxygen repellence, UV shielding, and ethylene scavenger and antimicrobial activity.
In the last years, the choice of a polymer suitable and environmentally sustainable for food packaging has oriented towards biopolymers, which can be easily degraded and recycled, for a more ecological fingerprint in a field that has been, so far, heavily relying on petroleum-based polymers. Such biopolymers include poly hydroxybutyrates (PHB), polylactic acid (PLA), poly caprolactone (PCL), polyvinyl alcohol (PVA), poly butylene succinate, lipids (wax and free fatty acids), proteins (casein, whey, and gluten), polysaccharides (starch and cellulose derivatives, alginates, and chitosan) and their possible blends [180]. In particular, chitosan, a polysaccharide derived from chitin, is especially suited for food packaging, considering, as reported above, its biocompatibility, biodegradability, and antibacterial and antifungal behavior, along with good film forming properties [177,181].
Zhang et al. developed a coating for food packaging based on TiO 2 NPs embedded in a chitosan (CS) matrix. The presence of TiO 2 NPs in a chitosan film served to improve, upon exposure to visible light, its mechanical properties, wettability and antibacterial activity against typical food pathogens (E. coli, St. aureus, C. albicans, and A. niger) with respect to film formed by bare chitosan. The effect of the direct application of the CS/TiO 2 coating on red grapes was also tested and was shown to preserve the fruit better than conventional plastic coating [181]. Li et al. reported a composite film where Ag-TiO 2 nanocomposites where embedded in a mixture of fish gelatin (FG) and CS, given that FG is a protein with good film forming, and antioxidant and water locking properties. The addition of CS imparted to FG enhanced mechanical properties while the integration of the Ag-TiO 2 nanocomposite, to a different extent, conveyed antibacterial activity. Antibacterial activity (against E. coli, St. aureus, and Botrytis cinereal) and UV-blocking function were found to increase as a function of Ag-TiO 2 concentration. However, its very high content led to a deterioration of the mechanical properties of the composite, that were observed, which instead, improved at low Ag-TiO 2 concentration [182]. Xu et al. realized a coating based on three different antimicrobial agents: graphene oxide (GO), chitosan and TiO 2 NPs, in increasing amounts. The sample with intermediate TiO 2 NPs concentration (GO:CS:TiO 2 ratio of 1:20:4) showed a good antimicrobial activity against B. subtilis and A. niger, and proved able to destroy bacterial cell membrane and to avoid the formation of a biofilm. Such a coating, when applied to fruit and vegetables, such as strawberries and mango, was found suitable to delay weight loss, which is caused by deterioration, to reduce polyphenol oxidase (PPO)-the enzyme responsible for food browning-activity, and to increase superoxide dismutase (SOD) activity, which is usually high in low stress conditions for the fruit. [47] Besides chitosan-based blends, many other polymers have been used recently to develop antimicrobial composite coatings containing TiO 2 NPs for food packaging. Xie et al., for example, embedded TiO 2 NPs into three different biodegradable polymers: cellulose acetate (CA), polycaprolactone (PCL), and PLA. A higher compatibility of TiO 2 with CA and PLA was observed, along with improved film forming properties, resulting in uniform films. Moreover, the CA/TiO 2 -based films showed a high transparency, and good photocatalytic activity, as demonstrated by degradation of methylene blue upon irradiation with a UV-A light. The best antibacterial activity under the investigated experimental conditions, when compared to the other systems, was found against E. coli upon 2 h UV-A light irradiation with light intensity of 1.30 ± 0.15 mW/cm 2 , and was found to increase at higher TiO 2 concentration, achieving a 1.69 log CFU/mL reduction. Very low reduction was, instead, observed, even at the highest TiO 2 concentration, without irradiation [183]. Xie et al. thoroughly investigated the behavior of the best performing CA/TiO 2 coatings when the bacteria were inoculated under the coating itself and-upon UV-A exposure-a higher TiO 2 concentration was found to act as a screen, decreasing the intensity of the light reaching the inoculum and resulting in reduced antibacterial activity [184].
He et al. realized a uniform coating using fish skin gelatin and TiO 2 in different ratios (30:1, 20:1, 10:1). The film showed antibacterial activity increased when TiO 2 was embedded, reaching values of 54.38% and 44.89% for E. coli and St. aureus, respectively, for the sample with the highest TiO 2 content (10:1 ratio) after 2 h UV irradiation. Moreover, the presence of TiO 2 enhanced tensile strength and elongation at break and reduced water vapor permeability of the coating. The coating, transparent in the visible region, was found to act as a barrier against UV-C light [175]. Teymoupour et al. reported a composite coating based on a soluble soybean polysaccharide (SSPS) as biopolymer. The SSPS/TiO 2 coating, obtained at increasing TiO 2 concentration-0%, 1%, 3%, and 5% (w/w)-resulted in an increased antibacterial activity against both E. coli and St. aureus, being particularly effective against St. aureus. Properties such as water vapor permeability, oxygen permeability, and moisture content decreased upon the addition of TiO 2 , while the mechanical properties improved [185].
All the application of any kind of NPs in materials that come into contact with food must cope with NP migration and their release, as it may become a severe environmental hazard [177,178]. Although in the last decade many strategies have been proposed to improve quality standard for food-contact materials and to reduce its impact on the environment, the regulations concerning nanocomposite based coatings for food safety remain behind, thus further delaying their development up to market [179].

Conclusions and Perspectives
Pathogenic microorganisms have demonstrated the ability to spread easily throughout the world, thus potentially very severely threatening human health, as the current COVID-19 pandemic has shown in the last year. Therefore, inactivation of pathogenic microorganisms in water, on surfaces, and on food strongly demands the attention of the scientific community. Photocatalytic TiO 2 -based nanomaterials have demonstrated their potential to tackle pathogens through multiple paths, in particular by exploiting light (even sun or ambient light, in some cases) to photocatalytically generate ROSs able to kill them or inhibit their growth without using chemical products, that may, instead, be even more harmful for human health and the environment. However, to realistically make the great promise of these photocatalytic nanomaterials viable, it is necessary to easily access large amounts of TiO 2 -based nanomaterials with controlled size, crystalline phase, and surface chemistry. Such a consideration has been, therefore, briefly summarized in the first part of the review, reporting examples of promising synthetic approaches, specifically selected for their potential scalability towards TiO 2 -based nanomaterial production, thus ultimately showing the great contribution of modern materials science in providing an extremely rich toolbox for manufacturing nanomaterials with designed photocatalytic properties.
In the second part of the review, we critically analyzed selected approaches within the plethora of reported ones to investigate the antimicrobial properties of TiO 2 -based nanomaterials. While all the described examples have shown the capability of TiO 2 -based nanomaterials in inactivating bacteria, fungi, and viruses, the great variability of the experimental parameters (catalyst loading, light source and intensity, target microorganism, environmental conditions of microorganism growth, method of detection of antimicrobial efficacy) used in the antimicrobial tests does not allow an objective comparison of the reported results nor to trace a profile of antimicrobial activity efficiency across the different proposed and investigated TiO 2 -based nanostructured materials. In addition, the selection of the target microorganism needs to be carefully designed as a function of the intended application. Moreover, a more fundamental issue that makes it very complex to correctly interpret the outcome of experiments is given by the multiple mechanisms of TiO 2 NPs antimicrobial activity concomitantly occurring, that, as reported, could involve an intrinsic antimicrobial effect of TiO 2 -based nanomaterials in the dark. It follows a strong need for standardized protocols and methods specifically suited to quantitatively evaluate the photocatalytic antimicrobial activity of TiO 2 NPs, and thus compare the performance of the materials. In this way, it would be possible to highlight the role of the different inhibition mechanisms of the investigated materials, under the specific conditions. Finally, established and standardized characterization tools to elucidate photocatalytic antimicrobial activities would also fully empower a sound investigation of a systematic structure-function relationship that could be applied not only to TiO 2 -based nanomaterials but also to whole classes of photocatalytic nanomaterials.
Nonetheless, the results reported so far are promising, as can be inferred by the maturity level of the technological applications that, in a few cases, have been already obtained, as discussed in the third part of the review. Indeed, photocatalytic TiO 2 -based nanomaterials have been recently successfully exploited in the disinfection of urban wastewater, in the production of photocatalytic anti-fouling membrane for ultra-and nanofiltration, to prevent building materials from bio-fouling, for biomaterials disinfection in wound dressing application and mask filters, and for the disinfection of food packaging and processing materials.
In conclusion, photocatalytic TiO 2 -based nanomaterials hold great promise in the fight against a wide range of pathogenic microorganisms that can be effectively inactivated and destroyed in different matrices, including water, air, surfaces, and food, possibly also providing additional weapons against extremely harmful and emerging strains like the SARS-CoV viruses.

Conflicts of Interest:
The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.