Entomopathogenic Filamentous Fungi as Biocatalysts in Glycosylation of Methylﬂavonoids

: Flavonoids are known for their numerous biological activities; however, their pharmacological application is limited by poor bioavailability. Glycosides are usually more stable and more soluble in water and in this form, flavonoids are present in nature. Likewise, the presence of the methyl group in the flavonoid skeleton results in facilitated absorption and greater bioavailability. Entomopathogenic filamentous fungi are effective in the biotransformation of flavonoids; they are known especially for efficient glycosylation. In the current study we used strains of Beauveria bassiana KCH J1.5 and Isaria fumosorosea KCH J2 to biotransform flavonoids with a single methyl group. was biotransformed by both strains into 2 (cid:48) -hydroxy-5 (cid:48) -methylchalcone 3- O - β -D-(4 (cid:48)(cid:48) - O -methyl)-glucopyranoside. Inthecultureof B.bassiana KCHJ1.5fourproductswereobtained from 6-methylflavanone: 4 (cid:48) -hydroxy-6-methylflavanone 3 (cid:48) - O - β -D-(4 (cid:48)(cid:48) - O -methyl)-glucopyranoside; 4 (cid:48) -hydroxyflavanone 6-methylene- O - β -D-(4 (cid:48)(cid:48) - O -methyl)-glucopyranoside; 6-hydroxymethylflavanone 3 (cid:48) - O - β -D-(4 (cid:48)(cid:48) - O -methyl)-glucopyranoside and 4 (cid:48) -hydroxy-6-hydroxymethylflavanone 3 (cid:48) - O - β -D-(4 (cid:48)(cid:48) O -methyl) -glucopyranoside. Biotransformation with I. fumosorosea KCH J2 as a biocatalyst resulted in the formation of 6-methylflavanone 4 (cid:48) - O - β -D-(4 (cid:48)(cid:48) - O -methyl)-glucopyranoside and 2-phenyl-6-methylchromane 4- O - β -D-(4 (cid:48)(cid:48) - O -methyl)-glucopyranoside. All of these flavonoids can be used in biological activity tests and can be useful in studies concerning structure—bioactivity relationships. from at C-6 from at and with signal from the proton at C-4 (cid:48) . Signals from one proton of the hydroxyl group at C-2 (cid:48) and three protons of


Introduction
Flavonoid compounds are plant secondary metabolites engaged in various plants' interactions with the environment [1]. They are able to exert a wide range of biological activities: antioxidant, anti-inflammatory, antiallergic, antimicrobial, antiplatelet, anticancer and neuroprotective [1][2][3]. Their occurrence in a plant based diet is ubiquitous [4]. However, pharmacological application of flavonoid aglycons is limited by their poor bioavailability [5,6]. In nature, flavonoid derivatives-products of glycosylation, methylation, prenylation, acetylation and polymerization-are very common [7] and the most abundant are glucosides. Addition of sugar moiety (or moieties) to flavonoid aglycone is catalyzed by uridine diphosphate (UDP) glycosyltransferases (UGTs) [8,9]. Flavonoids glycosylation results in the modulation of their physicochemical and biological properties, most of all by improving their aqueous solubility and facilitating their intracellular and intercellular transportation. Nonetheless, the impact of the glycosylation on the biological properties of flavonoids is complicated, because it depends on the structure of the flavonoid core, the position of the sugar attachment and the number of sugar moieties [10][11][12][13][14][15][16]. and aliphatic ketones, flavonoids and steroids [37]. This fungus is capable of performing reactions such as hydroxylation, glycosylation or deglycosylation, acetylation and reduction [37]. Most of the enzymes responsible for these reactions have not been described until now [37]. Likewise I. fumosorosea serves as a biological control agent and alternative to chemical pesticides [38]. Its secondary metabolites exhibit a great variety of biological activities [39]. This genus was used as a biocatalyst by our team in previous studies of flavonoid biotransformations [12][13][14][15].
The current studies are a continuation of our previous work with 6-methylflavone as the biotransformation substrate [12]. In the present study we synthesized two flavonoid compounds with methyl group at C-6 of flavanone skeleton (C-5′ in the case of chalcone) and afterwards biotransformed them with entomopathogenic filamentous fungi strains of B. bassiana KCH J1.5 and I. fumosorosea KCH J2 as biocatalysts. As a result of 2′-hydroxy-5′-methylchalcone biotransformation in both cultures one main product 2′-hydroxy-5′-methylchalcone 3-O-β-D-(4′'-O-methyl)glucopyranoside was obtained. 6-Methylflavanone was efficiently biotransformed by both used biocatalysts. Each strain formed different biotransformation products. Six flavanones 4′'-Omethylglucopyranosides were obtained. All biotransformation products have not been previously described in the scientific literature. The biotransformation products may be utilized in further studies as potentially biologically active compounds and can be useful in investigations concerning structure-bioactivity relationships.
In the present paper two flavonoid substrates (chalcone and flavanone), that are analogs of the 6-methylflavone, were utilized. Two strains of the enthompathogenic filamentous fungi I. fumosorosea KCH J2 and B. bassiana KCH J1.5, isolated from the environment and characterized previously [12,37] were used as biocatalysts. Experiments performed in a semi-preparative scale enabled us to determine the chemical structures of biotransformation products and their isolated yields unambiguously. Hereby, seven biotransformation products that   Scheme 1. Synthesis of 2′-hydroxy-5′-methylchalcone (3) and 6-methylflavanone (4).
The presence of a glucose unit in the compound 3a was confirmed by five characteristic carbon signals observed in the region from δ = 80.5 ppm to δ = 62.3 ppm in the Carbon-13 Nuclear Magnetic Resonance ( 13 C-NMR) spectrum, as well as proton signals of δH ranging from δ = 3.92 ppm to δ = 3.19 ppm in the Proton Nuclear Magnetic Resonance ( 1 H-NMR) spectrum. Moreover, the attachment of a sugar unit to substrate 3 was confirmed by a one-proton doublet from the proton at the anomeric carbon atom at δ = 5.03 ppm in the 1 H-NMR spectrum. The β-configuration of the glucose unit was proved by the coupling constant (J = 7.8 Hz) for the anomeric proton. A three-proton singlet at δ = 3.57 ppm in the 1 H-NMR spectrum and the corresponding signal at δ = 60.6 ppm in the 13 C-NMR spectrum evidences that one of the hydroxyl groups of sugar unit has been methylated. Omethylation occurred in the C-4′' hydroxyl group of the glucose unit. It was detected in the Heteronuclear Multiple Bond Coherence (HMBC) spectrum, where the proton signal due to -OCH3 was correlated with the signal of C-4′' (about δ = 80 ppm) in the glucose unit. Sugar moiety was attached to C-3, because in the HMBC spectrum signal due to the proton at hemiacetal carbon atom (δ = 5.03 ppm) was correlated with the C-3 signal (δ = 159.2 ppm), which was shifted from δ = 129.2 ppm, indicating the attachment of an electronegative atom. Additionally, protons in the 1 H-NMR spectrum at C-2 and C-6 became non-equivalent, and the proton at C-3 disappeared. Shifted signals can be observed: one multiplet from the proton at C-2 (δ = 7.61 ppm), which became isolated in the correlation spectrum, one doublet of doublets of doublets from the proton at C-4 (δ = 7.16 ppm) and one doublet from the proton at C-6 (δ = 7.47 ppm). Signal from proton at C-5 was slightly shifted and merged with signal from the proton at C-4′. Signals from one proton of the hydroxyl group at C-2′ and three protons of the methyl group at C-5 remained intact. The product 3a was analyzed by NMR spectroscopy that allowed to establish its chemical structure (Tables 1 and 2, Scheme 3).

O
The presence of a glucose unit in the compound 3a was confirmed by five characteristic carbon signals observed in the region from δ = 80.5 ppm to δ = 62.3 ppm in the Carbon-13 Nuclear Magnetic Resonance ( 13 C-NMR) spectrum, as well as proton signals of δH ranging from δ = 3.92 ppm to δ = 3.19 ppm in the Proton Nuclear Magnetic Resonance ( 1 H-NMR) spectrum. Moreover, the attachment of a sugar unit to substrate 3 was confirmed by a one-proton doublet from the proton at the anomeric carbon atom at δ = 5.03 ppm in the 1 H-NMR spectrum. The β-configuration of the glucose unit was proved by the coupling constant (J = 7.8 Hz) for the anomeric proton. A three-proton singlet at δ = 3.57 ppm in the 1 H-NMR spectrum and the corresponding signal at δ = 60.6 ppm in the 13 C-NMR spectrum evidences that one of the hydroxyl groups of sugar unit has been methylated. O-methylation occurred in the C-4 hydroxyl group of the glucose unit. It was detected in the Heteronuclear Multiple Bond Coherence (HMBC) spectrum, where the proton signal due to -OCH 3 was correlated with the signal of C-4 (about δ = 80 ppm) in the glucose unit. Sugar moiety was attached to C-3, because in the HMBC spectrum signal due to the proton at hemiacetal carbon atom (δ = 5.03 ppm) was correlated with the C-3 signal (δ = 159.2 ppm), which was shifted from δ = 129.2 ppm, indicating the attachment of an electronegative atom. Additionally, protons in the 1 H-NMR spectrum at C-2 and C-6 became non-equivalent, and the proton at C-3 disappeared. Shifted signals can be observed: one multiplet from the proton at C-2 (δ = 7.61 ppm), which became isolated in the correlation spectrum, one doublet of doublets of doublets from the proton at C-4 (δ = 7.16 ppm) and one doublet from the proton at C-6 (δ = 7.47 ppm). Signal from proton at C-5 was slightly shifted and merged with signal from the proton at C-4 . Signals from one proton of the hydroxyl group at C-2 and three protons of the methyl group at C-5 remained intact.
The presence of a glucose unit in the compound 3a was confirmed by five characteristic carbon signals observed in the region from δ = 80.5 ppm to δ = 62.3 ppm in the Carbon-13 Nuclear Magnetic Resonance ( 13 C-NMR) spectrum, as well as proton signals of δH ranging from δ = 3.92 ppm to δ = 3.19 ppm in the Proton Nuclear Magnetic Resonance ( 1 H-NMR) spectrum. Moreover, the attachment of a sugar unit to substrate 3 was confirmed by a one-proton doublet from the proton at the anomeric carbon atom at δ = 5.03 ppm in the 1 H-NMR spectrum. The β-configuration of the glucose unit was proved by the coupling constant (J = 7.8 Hz) for the anomeric proton. A three-proton singlet at δ = 3.57 ppm in the 1 H-NMR spectrum and the corresponding signal at δ = 60.6 ppm in the 13 C-NMR spectrum evidences that one of the hydroxyl groups of sugar unit has been methylated. Omethylation occurred in the C-4′' hydroxyl group of the glucose unit. It was detected in the Heteronuclear Multiple Bond Coherence (HMBC) spectrum, where the proton signal due to -OCH3 was correlated with the signal of C-4′' (about δ = 80 ppm) in the glucose unit. Sugar moiety was attached to C-3, because in the HMBC spectrum signal due to the proton at hemiacetal carbon atom (δ = 5.03 ppm) was correlated with the C-3 signal (δ = 159.2 ppm), which was shifted from δ = 129.2 ppm, indicating the attachment of an electronegative atom. Additionally, protons in the 1 H-NMR spectrum at C-2 and C-6 became non-equivalent, and the proton at C-3 disappeared. Shifted signals can be observed: one multiplet from the proton at C-2 (δ = 7.61 ppm), which became isolated in the correlation spectrum, one doublet of doublets of doublets from the proton at C-4 (δ = 7.16 ppm) and one doublet from the proton at C-6 (δ = 7.47 ppm). Signal from proton at C-5 was slightly shifted and merged with signal from the proton at C-4′. Signals from one proton of the hydroxyl group at C-2′ and three protons of the methyl group at C-5 remained intact.   Glycosylation of chalcones is rarely reported. Among reported microbial transformations of the chalcones into their glycosides, there are known reactions with xanthohumol as a substrate. These biotransformations led to the production of: xanthohumol 4 -O-β-D-glucopyranoside in the cultures of Penicillium chrysogenum 6933 [40], Absidia coerulea AM93 and Rhizopus nigricans UPF701 [41], xanthohumol 4,4 -O-β-diglucopyranoside in the culture of P. chrysogenum 6933 [40], xanthohumol 4 -O-β-D-(4 -O-methyl)-glucopyranoside in the cultures of B. bassiana AM278 [42] and B. bassiana AM446 [41], and isoxanthohumol 7-O-β-D-glucopyranoside in the cultures of Cunninghamella elegans 6992 [40] and Mortierella mutabilis AM404 [41]. Several studies with enzymes of the glycosyltransferase family led to the formation of the chalcone glycosides at three sites: 2 , 4 and less often 4 [43]. However, there is no evidence of the glycosylation at C-3 of the chalcone skeleton and also no reports of 2 -hydroxy-5 -methylchalcone (3) biotransformations. The products 4a-4d were analyzed by NMR spectroscopy which allowed for the establishment of their chemical structures (Tables 3 and 4, Schemes 5-8).
The presence of a glucose unit in compounds 4a-4d was established by five characteristic carbon signals observed in the region from about δ = 80.0 ppm to about δ = 62.0 ppm in the 13 C-NMR spectra, as well as proton signals of δH ranging from about δ = 3.9 ppm to δ = 3.1 ppm in the 1 H-NMR spectra. Additionally, the attachment of a sugar unit to substrate 4 was confirmed by a one-proton doublet from the proton at the anomeric carbon atom in the 1 H-NMR spectrum of 4a at δ = 4.79 ppm, 4b at δ = 4.36 ppm, 4c at δ = 4.98 ppm and 4d at δ = 4.82 ppm. The β-configuration of the glucose unit was proved by the coupling constant (about J = 7.9 Hz) for the anomeric proton. A three-proton singlet at about δ = 3.6 ppm in the 1 H-NMR spectra and the corresponding signal at about δ = 60.6 ppm in the 13 C-NMR spectra of 4a-4d evidences that one of the hydroxyl groups of sugar unit has been methylated. O-methylation occurred in all products at the C-4′' hydroxyl group of the glucose unit. It was detected in the HMBC spectra, that the proton signals due to -OCH3, were correlated with the signals of C-4′' (about δ = 80 ppm) in the glucose units.
Based on the HMBC spectrum, a sugar moiety was attached to C-3′ in the case of compound 4a, due to the proton at the anomeric carbon atom (δ = 4.79 ppm) being coupled with the C-3′ signal (δ = 146.1 ppm), which was shifted from δ = 129.5 ppm, indicating the attachment of an electronegative atom. Moreover, protons in the 1 H-NMR spectrum at C-2′ and C-6′ became non-equivalent and protons at C-3′ and C-4′ were not present, suggesting substitution. The C-4′ signal at the 13 C-NMR spectrum (δ = 149.0 ppm), which was shifted from δ = 129.3 ppm, indicates the attachment of an electronegative oxygen atom from a hydroxyl group. In the 1 H-NMR spectrum shifted signals of flavanone ring B can be observed: one multiplet from the proton at C-2′ (δ = 7.39 ppm), one doublet from the proton at C-5′ (δ = 6.91 ppm), and one doublet of triplets at C-6′ (δ = 7.16 ppm). In the COSY spectrum protons at C-5′ and at C-6′correlate, and the proton at C-2′ does not couple with any proton, which also confirms substitution at C-3′ and C-4′. In the HMBC spectrum, a strong cross-peak between C-3′ and H-2′ and between C-3′ and H-5′, while no peak between C-3′ and H-6′, can be observed. At the same time a strong cross-peak between C-4′ and H-2′ (and H-5′ and H-6′) can be seen. Cross-peak by three chemical bonds occurs less likely than by two chemical bonds in HMBC experiments. Therefore, we assigned the C-3′ signal as having cross-peaks by one, and two chemical bonds, and the C-4′ signal as having cross-peaks by one and two chemical bonds [44][45][46]. Chemical shifts of the other signals in the 1 H-NMR and 13 C-NMR spectra have only slightly changed. The signal from the three protons of the methyl group at C-6 remained at the same position as in the 1 H-NMR spectrum of substrate 4 which indicates the presence of the methyl group in product 4a. The products 4a-4d were analyzed by NMR spectroscopy which allowed for the establishment of their chemical structures (Tables 3 and 4, Schemes 5-8).
The presence of a glucose unit in compounds 4a-4d was established by five characteristic carbon signals observed in the region from about δ = 80.0 ppm to about δ = 62.0 ppm in the 13 C-NMR spectra, as well as proton signals of δH ranging from about δ = 3.9 ppm to δ = 3.1 ppm in the 1 H-NMR spectra. Additionally, the attachment of a sugar unit to substrate 4 was confirmed by a one-proton doublet from the proton at the anomeric carbon atom in the 1 H-NMR spectrum of 4a at δ = 4.79 ppm, 4b at δ = 4.36 ppm, 4c at δ = 4.98 ppm and 4d at δ = 4.82 ppm. The β-configuration of the glucose unit was proved by the coupling constant (about J = 7.9 Hz) for the anomeric proton. A three-proton singlet at about δ = 3.6 ppm in the 1 H-NMR spectra and the corresponding signal at about δ = 60.6 ppm in the 13 C-NMR spectra of 4a-4d evidences that one of the hydroxyl groups of sugar unit has been methylated. O-methylation occurred in all products at the C-4 hydroxyl group of the glucose unit. It was detected in the HMBC spectra, that the proton signals due to -OCH 3 , were correlated with the signals of C-4 (about δ = 80 ppm) in the glucose units.
Based on the HMBC spectrum, a sugar moiety was attached to C-3 in the case of compound 4a, due to the proton at the anomeric carbon atom (δ = 4.79 ppm) being coupled with the C-3 signal (δ = 146.1 ppm), which was shifted from δ = 129.5 ppm, indicating the attachment of an electronegative atom. Moreover, protons in the 1 H-NMR spectrum at C-2 and C-6 became non-equivalent and protons at C-3 and C-4 were not present, suggesting substitution. The C-4 signal at the 13 C-NMR spectrum (δ = 149.0 ppm), which was shifted from δ = 129.3 ppm, indicates the attachment of an electronegative oxygen atom from a hydroxyl group. In the 1 H-NMR spectrum shifted signals of flavanone ring B can be observed: one multiplet from the proton at C-2 (δ = 7.39 ppm), one doublet from the proton at C-5 (δ = 6.91 ppm), and one doublet of triplets at C-6 (δ = 7.16 ppm). In the COSY spectrum protons at C-5 and at C-6 correlate, and the proton at C-2 does not couple with any proton, which also confirms substitution at C-3 and C-4 . In the HMBC spectrum, a strong cross-peak between C-3 and H-2 and between C-3 and H-5 , while no peak between C-3 and H-6 , can be observed. At the same time a strong cross-peak between C-4 and H-2 (and H-5 and H-6 ) can be seen. Cross-peak by three chemical bonds occurs less likely than by two chemical bonds in HMBC experiments. Therefore, we assigned the C-3 signal as having cross-peaks by one, and two chemical bonds, and the C-4 signal as having cross-peaks by one and two chemical bonds [44][45][46]. Chemical shifts of the other signals in the 1 H-NMR and 13 C-NMR spectra have only slightly changed. The signal from the three protons of the methyl group at C-6 remained at the same position as in the 1 H-NMR spectrum of substrate 4 which indicates the presence of the methyl group in product 4a.  In the case of compound 4b, the sugar moiety was attached to the hydroxyl group-previously formed by microbial oxidation of the methyl group-because in the HMBC spectrum the proton at the anomeric carbon atom (δ = 4.78 ppm) was coupled with the C-6-CH2-signal (δ = 70.3 ppm). Moreover, the signal from the three protons of the methyl group at C-6 present in the 1 H-NMR spectrum (δ = 2.32 ppm) of substrate 4 disappeared and two signals appeared from protons at 4.86 ppm and 4.61 ppm (unequal protons from methylene group), which confirms attachment of the glucose unit to the methylene bridge at C-6. In the 1 H-NMR spectrum, proton signals of the flavanone ring A became only slightly shifted because of it. Moreover, signals from protons of the ring B became shifted, indicating another substitution in ring B. Two multiplets from the protons at C-2' and C-6' (δ = 7.41 ppm) and at C-3' and C-5' (δ = 6.90 ppm) were observed and the signal from proton at C-4' was Scheme 5. Key COSY (on the left) and HMBC (on the right) correlations for the structure elucidation of product 4a.
In the case of compound 4b, the sugar moiety was attached to the hydroxyl group-previously formed by microbial oxidation of the methyl group-because in the HMBC spectrum the proton at the anomeric carbon atom (δ = 4.78 ppm) was coupled with the C-6-CH 2 -signal (δ = 70.3 ppm). Moreover, the signal from the three protons of the methyl group at C-6 present in the 1 H-NMR spectrum (δ = 2.32 ppm) of substrate 4 disappeared and two signals appeared from protons at 4.86 ppm and 4.61 ppm (unequal protons from methylene group), which confirms attachment of the glucose unit to the methylene bridge at C-6. In the 1 H-NMR spectrum, proton signals of the flavanone ring A became only slightly shifted because of it. Moreover, signals from protons of the ring B became shifted, indicating another substitution in ring B. Two multiplets from the protons at C-2' and C-6' (δ = 7.41 ppm) and at C-3' and C-5' (δ = 6.90 ppm) were observed and the signal from proton at C-4' was missing which indicates a substitution at C-4'. In the 13 C-NMR spectrum C-4 signal (δ = 158.7 ppm), which was shifted from δ = 129.3 ppm, indicates the attachment of a hydroxyl group. Chemical shifts of the other signals in the 1 H-NMR and 13 C-NMR spectra have only slightly changed that signalize that the flavanone skeleton remained intact. of product 4a.
In the case of compound 4b, the sugar moiety was attached to the hydroxyl group-previously formed by microbial oxidation of the methyl group-because in the HMBC spectrum the proton at the anomeric carbon atom (δ = 4.78 ppm) was coupled with the C-6-CH2-signal (δ = 70.3 ppm). Moreover, the signal from the three protons of the methyl group at C-6 present in the 1 H-NMR spectrum (δ = 2.32 ppm) of substrate 4 disappeared and two signals appeared from protons at 4.86 ppm and 4.61 ppm (unequal protons from methylene group), which confirms attachment of the glucose unit to the methylene bridge at C-6. In the 1 H-NMR spectrum, proton signals of the flavanone ring A became only slightly shifted because of it. Moreover, signals from protons of the ring B became shifted, indicating another substitution in ring B. Two multiplets from the protons at C-2' and C-6' (δ = 7.41 ppm) and at C-3' and C-5' (δ = 6.90 ppm) were observed and the signal from proton at C-4' was missing which indicates a substitution at C-4'. In the 13 C-NMR spectrum C-4′ signal (δ = 158.7 ppm), which was shifted from δ = 129.3 ppm, indicates the attachment of a hydroxyl group. Chemical shifts of the other signals in the 1 H-NMR and 13 C-NMR spectra have only slightly changed that signalize that the flavanone skeleton remained intact. Based on the HMBC spectrum, in compound 4c, a sugar moiety was attached at C-3', due to the proton at the anomeric carbon atom (δ = 4.98 ppm) being coupled with the C-3' signal (δ = 158.9 ppm), which was shifted from δ = 129.5 ppm, and points out the attachment of an electronegative atom. Moreover, protons in the 1 H-NMR spectrum at C-2′ and C-6′ became non-equivalent. In the 1 H-NMR spectrum shifted proton signals of ring B can be observed: multiplet from proton at C-2' (δ = 7.29 ppm), triplet from proton at C-5' (δ = 7.35 ppm) and doublet from proton at C-6' (δ = 7.21 ppm), and signal from proton at C-4' that overlapped with the signal from proton at C-8 (δ = 7.07 ppm). In the COSY spectrum proton at C-2' became isolated, confirming substitution at C-3′. Moreover, the signal from the three protons of the methyl group at C-6 present in the 1 H-NMR spectrum (δ = 2.32 ppm) of substrate 4 disappeared. The presence of another moiety-a hydroxymethyl group at C-6 can be noticed in the 13 C-NMR spectrum, because the signal from C-6 was shifted from δ = 131.6 ppm to δ = 136.7 ppm. It was also confirmed by the appearance of a signal from two protons at 4.61 ppm [47] and slightly shifted signals from protons of ring A: doublet from the proton at C-5 (δ = 7.82 ppm), doublet of doublets from the proton at C-7 (δ = 7.58 ppm) and doublet of doublets from the proton at C-8 (δ = 7.07 ppm). Chemical shifts of the other signals in the 1 H-NMR and 13 C-NMR spectra have only slightly changed which indicates that the flavanone skeleton remained unaffected. Based on the HMBC spectrum, in compound 4c, a sugar moiety was attached at C-3', due to the proton at the anomeric carbon atom (δ = 4.98 ppm) being coupled with the C-3' signal (δ = 158.9 ppm), which was shifted from δ = 129.5 ppm, and points out the attachment of an electronegative atom. Moreover, protons in the 1 H-NMR spectrum at C-2 and C-6 became non-equivalent. In the 1 H-NMR spectrum shifted proton signals of ring B can be observed: multiplet from proton at C-2' (δ = 7.29 ppm), triplet from proton at C-5' (δ = 7.35 ppm) and doublet from proton at C-6' (δ = 7.21 ppm), and signal from proton at C-4' that overlapped with the signal from proton at C-8 (δ = 7.07 ppm). In the COSY spectrum proton at C-2' became isolated, confirming substitution at C-3 . Moreover, the signal from the three protons of the methyl group at C-6 present in the 1 H-NMR spectrum (δ = 2.32 ppm) of substrate 4 disappeared. The presence of another moiety-a hydroxymethyl group at C-6 can be noticed in the 13 C-NMR spectrum, because the signal from C-6 was shifted from δ = 131.6 ppm to δ = 136.7 ppm. It was also confirmed by the appearance of a signal from two protons at 4.61 ppm [47] and slightly shifted signals from protons of ring A: doublet from the proton at C-5 (δ = 7.82 ppm), doublet of doublets from the proton at C-7 (δ = 7.58 ppm) and doublet of doublets from the proton at C-8 (δ = 7.07 ppm). Chemical shifts of the other signals in the 1 H-NMR and 13 C-NMR spectra have only slightly changed which indicates that the flavanone skeleton remained unaffected. Based on the HMBC spectrum, a sugar moiety was attached to C-3′ in the case of compound 4d, due to the proton at the anomeric carbon atom (δ = 4.81 ppm) being coupled with the C-3′ signal (δ = 146.1 ppm), which was shifted from δ = 129.5 ppm. Additionally, protons in the 1 H-NMR spectrum at C-2′ and C-6′ became non-equivalent. Shifted signals can be observed: one doublet of doublets from the proton at C-2' (δ = 7.41 ppm), one doublet of triplets at C-6′ (δ = 7.17 ppm), and one doublet from the proton at C-5' (δ = 6.92 ppm). In the 13 C-NMR spectrum signal from C-4' (δ = 149.0 ppm) and signal from C-6 (δ = 136.5 ppm) was also shifted compared with the signals from substrate 4 δ = 129.3 ppm and δ = 131.6 ppm, respectively. These changes indicate the introduction of a hydroxyl group at C-4′ and hydroxymethyl group at C-6. Methylglucosyl moiety was attached to C-3′ and hydroxyl Based on the HMBC spectrum, a sugar moiety was attached to C-3 in the case of compound 4d, due to the proton at the anomeric carbon atom (δ = 4.81 ppm) being coupled with the C-3 signal (δ = 146.1 ppm), which was shifted from δ = 129.5 ppm. Additionally, protons in the 1 H-NMR spectrum at C-2 and C-6 became non-equivalent. Shifted signals can be observed: one doublet of doublets from the proton at C-2' (δ = 7.41 ppm), one doublet of triplets at C-6 (δ = 7.17 ppm), and one doublet from the proton at C-5' (δ = 6.92 ppm). In the 13 C-NMR spectrum signal from C-4' (δ = 149.0 ppm) and signal from C-6 (δ = 136.5 ppm) was also shifted compared with the signals from substrate 4 δ = 129.3 ppm and δ = 131.6 ppm, respectively. These changes indicate the introduction of a hydroxyl group at C-4 and hydroxymethyl group at C-6. Methylglucosyl moiety was attached to C-3 and hydroxyl moiety to C-4 same as in product 4b, which was confirmed by cross-peaks in the HMBC spectrum [44][45][46]. Signals from the protons of ring A were only slightly changed, but exchange of a methyl group on the hydroxymethyl group at C-6 was confirmed by the disappearance of the signal from the three protons of the methyl moiety that was present in the 1 H-NMR spectrum (δ = 2.32 ppm) of substrate 4 and the appearance of a signal from two protons at 4.61 ppm [47]. Scheme 7. Key COSY (on the left) and HMBC (on the right) correlations for the structure elucidation of product 4c.
Based on the HMBC spectrum, a sugar moiety was attached to C-3′ in the case of compound 4d, due to the proton at the anomeric carbon atom (δ = 4.81 ppm) being coupled with the C-3′ signal (δ = 146.1 ppm), which was shifted from δ = 129.5 ppm. Additionally, protons in the 1 H-NMR spectrum at C-2′ and C-6′ became non-equivalent. Shifted signals can be observed: one doublet of doublets from the proton at C-2' (δ = 7.41 ppm), one doublet of triplets at C-6′ (δ = 7.17 ppm), and one doublet from the proton at C-5' (δ = 6.92 ppm). In the 13 C-NMR spectrum signal from C-4' (δ = 149.0 ppm) and signal from C-6 (δ = 136.5 ppm) was also shifted compared with the signals from substrate 4 δ = 129.3 ppm and δ = 131.6 ppm, respectively. These changes indicate the introduction of a hydroxyl group at C-4′ and hydroxymethyl group at C-6. Methylglucosyl moiety was attached to C-3′ and hydroxyl moiety to C-4′ same as in product 4b, which was confirmed by cross-peaks in the HMBC spectrum [44][45][46]. Signals from the protons of ring A were only slightly changed, but exchange of a methyl group on the hydroxymethyl group at C-6 was confirmed by the disappearance of the signal from the three protons of the methyl moiety that was present in the 1 H-NMR spectrum (δ = 2.32 ppm) of substrate 4 and the appearance of a signal from two protons at 4.61 ppm [47].     The products 4e and 4f were analyzed by 1 H-NMR and 13 C-NMR spectroscopy which allowed for the establishment of their chemical structures (Tables 3 and 4, Schemes 11 and 12).
The presence of a glucose unit in compounds 4e and 4f was confirmed by five characteristic carbon signals observed in the region from about δ = 80.0 ppm, to about δ = 62.0 ppm, in the 13 C-NMR spectra, as well as proton signals of δH ranging from about δ = 3.9 ppm, to δ = 3.1 ppm in the 1 H-NMR spectra. Additionally, the attachment of a sugar unit to substrate 4 was confirmed by a oneproton doublet at the anomeric carbon atom at δ = 4.49 ppm in the 1 H-NMR spectrum of 4e, δ = 4.97 ppm in the 1 H-NMR spectrum of 4f. The β-configuration of the glucose unit was proved for 4e and 4f by the coupling constant (J = 7.8 Hz) for the anomeric proton. A three-proton singlet at about δ = 3.6 ppm in the 1 H-NMR spectra and the corresponding signal at δ = 60.5 ppm in the 13 C-NMR spectra of 4e and 4f evidences that one of the hydroxyl groups of sugar unit has been methylated. Omethylation occurred at the C-4′' hydroxyl group of the glucose unit. It was detected in the HMBC spectrum, where the proton signal due to -OCH3 was correlated with the signal of C-4′' (about δ = 80 ppm) in the glucose unit.
Based on the HMBC spectrum, a sugar moiety was attached to C-4′ in the case of compound 4e, due to the proton at the anomeric carbon atom (δ = 4.78 ppm) being coupled with the C-4′ signal (δ = 158.8 ppm), which was shifted from δ = 129.3 ppm. In the 1 H-NMR spectrum, multiplets from protons at C-2′ and C-6′ (δ = 7.49 ppm) and from protons at C-3′ and C-5′ (δ = 7.12 ppm) can be observed, which is characteristic of an AA'BB' coupling system, signalizing the presence of flavanone ring B substitution at C-4′. Chemical shifts of the other signals in the 1 H-NMR and 13 C-NMR spectra have only slightly changed, which indicates that the flavanone skeleton remained intact. The signal from The products 4e and 4f were analyzed by 1 H-NMR and 13 C-NMR spectroscopy which allowed for the establishment of their chemical structures (Tables 3 and 4, Schemes 11 and 12). Based on the HMBC spectrum, in the case of product 4f, glycosylation occurred at C-4,due to the proton at the anomeric carbon atom (δ = 4.49 ppm) being coupled with the C-4 signal (δ = 70.2 ppm), which was shifted from δ = 191.9 ppm, indicating reduction of the carbonyl group at C-4. Furthermore, in the 1 H-NMR spectrum, a triplet at δ = 4.92 ppm appeared from one-proton at C-4. Moreover, only slightly shifted signals from all ten protons from substrate 4 were visible, confirming the inability of another position of substitution. A doublet of doublets at δ = 5.36 ppm from one proton at C-2 became shifted from δ = 5.59 ppm, the same as the signals from the pseudo-axial and pseudoequatorial protons at C-3, respectively (δ =2.43 ppm shifted from δ = 3.11 ppm and δ = 2.07 ppm shifted from δ = 2.85 ppm). In the COSY spectrum there was a correlation between proton 3ax and 3eq with a new signal from the proton at C-4. In the HMBC spectrum correlations between the proton at C-5 and the carbon at C-4, and between the pseudo-axial proton at C-3 and the carbon at C-4, and between the proton at C-1′' and the carbon at C-4 were also present, which confirms that reduction and glycosylation occurred at C-4. The signal from the three protons of the methyl group at C-6 remained at the same position as in the 1 H-NMR spectrum of substrate 4 which indicates the presence of the methyl group in product 4f.  Based on the HMBC spectrum, in the case of product 4f, glycosylation occurred at C-4,due to the proton at the anomeric carbon atom (δ = 4.49 ppm) being coupled with the C-4 signal (δ = 70.2 ppm), which was shifted from δ = 191.9 ppm, indicating reduction of the carbonyl group at C-4. Furthermore, in the 1 H-NMR spectrum, a triplet at δ = 4.92 ppm appeared from one-proton at C-4. Moreover, only slightly shifted signals from all ten protons from substrate 4 were visible, confirming the inability of another position of substitution. A doublet of doublets at δ = 5.36 ppm from one proton at C-2 became shifted from δ = 5.59 ppm, the same as the signals from the pseudo-axial and pseudoequatorial protons at C-3, respectively (δ =2.43 ppm shifted from δ = 3.11 ppm and δ = 2.07 ppm shifted from δ = 2.85 ppm). In the COSY spectrum there was a correlation between proton 3ax and 3eq with a new signal from the proton at C-4. In the HMBC spectrum correlations between the proton at C-5 and the carbon at C-4, and between the pseudo-axial proton at C-3 and the carbon at C-4, and between the proton at C-1′' and the carbon at C-4 were also present, which confirms that reduction and glycosylation occurred at C-4. The signal from the three protons of the methyl group at C-6 remained at the same position as in the 1 H-NMR spectrum of substrate 4 which indicates the presence of the methyl group in product 4f. The first product of the biotransformations presented above-6-methylflavanone 4′-O-β-D-(4′'-O-methyl)-glucopyranoside (4e)-is analogous to the 6-methoxyflavanone 4′-O-β-D-(4"-O-methyl)glucopyranoside that was obtained in the microbial transformations of 6-methoxyflavanone by the same strain, I. fumosorosea KCH J2 [15]. Glycosylation at C-4′ also occurred in the biotransformation of naringenin in the culture of I. fumosorosea ACCC 37814, performed by Dou et al. [16]. More unusual, is the second biotransformation product-2-phenyl-6-methylchromane 4-O-β-D-(4′'-O-methyl)glucopyranoside (4f). We assume that attachment of the glycosyl moiety at C-4 was preceded by the carbonyl group reduction, which was previously observed as a result of 2′-methoxyflavanone and 3′methoxyflavanone biotransformations [15]. However, subsequent glycosylation at the same position has never been observed before. In each biotransformation product a methyl moiety at C-6 was retained, indicating that I. fumosorosea KCH J2 was not able to remove this moiety, unlike B. bassiana KCH J1.5. The presence of a glucose unit in compounds 4e and 4f was confirmed by five characteristic carbon signals observed in the region from about δ = 80.0 ppm, to about δ = 62.0 ppm, in the 13 C-NMR spectra, as well as proton signals of δH ranging from about δ = 3.9 ppm, to δ = 3.1 ppm in the 1 H-NMR spectra. Additionally, the attachment of a sugar unit to substrate 4 was confirmed by a one-proton doublet at the anomeric carbon atom at δ = 4.49 ppm in the 1 H-NMR spectrum of 4e, δ = 4.97 ppm in the 1 H-NMR spectrum of 4f. The β-configuration of the glucose unit was proved for 4e and 4f by the coupling constant (J = 7.8 Hz) for the anomeric proton. A three-proton singlet at about δ = 3.6 ppm in the 1 H-NMR spectra and the corresponding signal at δ = 60.5 ppm in the 13 C-NMR spectra of 4e and 4f evidences that one of the hydroxyl groups of sugar unit has been methylated. O-methylation occurred at the C-4 hydroxyl group of the glucose unit. It was detected in the HMBC spectrum, where the proton signal due to -OCH 3 was correlated with the signal of C-4 (about δ = 80 ppm) in the glucose unit.
Based on the HMBC spectrum, a sugar moiety was attached to C-4 in the case of compound 4e, due to the proton at the anomeric carbon atom (δ = 4.78 ppm) being coupled with the C-4 signal (δ = 158.8 ppm), which was shifted from δ = 129.3 ppm. In the 1 H-NMR spectrum, multiplets from protons at C-2 and C-6 (δ = 7.49 ppm) and from protons at C-3 and C-5 (δ = 7.12 ppm) can be observed, which is characteristic of an AA'BB' coupling system, signalizing the presence of flavanone ring B substitution at C-4 . Chemical shifts of the other signals in the 1 H-NMR and 13 C-NMR spectra have only slightly changed, which indicates that the flavanone skeleton remained intact. The signal from the three protons of the methyl group at C-6 remained at the same position as in the 1 H-NMR spectrum of substrate 4, which means that the methyl group in product 4e was present.
Based on the HMBC spectrum, in the case of product 4f, glycosylation occurred at C-4, due to the proton at the anomeric carbon atom (δ = 4.49 ppm) being coupled with the C-4 signal (δ = 70.2 ppm), which was shifted from δ = 191.9 ppm, indicating reduction of the carbonyl group at C-4. Furthermore, in the 1 H-NMR spectrum, a triplet at δ = 4.92 ppm appeared from one-proton at C-4. Moreover, only slightly shifted signals from all ten protons from substrate 4 were visible, confirming the inability of another position of substitution. A doublet of doublets at δ = 5.36 ppm from one proton at C-2 became shifted from δ = 5.59 ppm, the same as the signals from the pseudo-axial and pseudo-equatorial protons at C-3, respectively (δ =2.43 ppm shifted from δ = 3.11 ppm and δ = 2.07 ppm shifted from δ = 2.85 ppm). In the COSY spectrum there was a correlation between proton 3 ax and 3 eq with a new signal from the proton at C-4. In the HMBC spectrum correlations between the proton at C-5 and the carbon at C-4, and between the pseudo-axial proton at C-3 and the carbon at C-4, and between the proton at C-1 and the carbon at C-4 were also present, which confirms that reduction and glycosylation occurred at C-4. The signal from the three protons of the methyl group at C-6 remained at the same position as in the 1 H-NMR spectrum of substrate 4 which indicates the presence of the methyl group in product 4f.
The physical data, including the retention time t R (min), the optical rotation [α] 20 D , and concentration c (M), as well as NMR spectral data of the resulting compounds 3 and 4 are presented below, and in Tables 1-4 Table 3, 13 C-NMR, see Table 4.

Microorganisms
The studies were carried out using two strains of entomopathogenic filamentous fungi I. fumosorosea KCH J2 and B. bassiana KCH J1.5, that were collected from the Department of Chemistry of Wrocław University of Environmental and Life Sciences, Poland. The description of material collection, propagation of structures of the fungi, and genetic identification have already been described in our previous papers [12,37]. The microorganisms were maintained on potato slants at 4 • C and subcultured before use in the experiments.

Analysis
The course of the biotransformation was assessed by chromatographic methods (TLC, HPLC). TLC analysis was carried out using TLC Silica gel 60/Kieselguhr F254 (0.2 mm thick) plates (Merck, Darmstadt, Germany). The developing system was a mixture of chloroform and methanol (9:1 v/v). The products were observed (without additional visualization) under the ultraviolet lamp at the two wavelengths 254 nm and 365 nm.
HPLC analyses were performed on a Dionex Ultimate 3000 instrument (Thermo Fisher Scientfic, Waltham, MA, USA) with a diode array detector using an analytical octadecyl silica (ODS) 2 column (4.6 × 250 mm, Waters, Milford, MA, USA) and pre-column. The gradient program was as follows: initial conditions-32.5% B in A, 4 min-40% B in A, 8 min-40% B in A, 10 min-45% B in A, 15 min-95% B in A, 18 min-95% B in A, 19 min-32.5% B in A, 23 min-32.5% B in A. The flow rate was 1 mL/min, the injection volume was 5 µL, and detection wavelength 280 nm.
Separation of the products obtained by the scale-up biotransformation was attained using 500 and 1000 µm preparative TLC silica gel plates (Analtech, Gehrden, Germany). After elution of the compounds from the adsorbent on TLC plates with chloroform and methanol (9:1 v/v) as eluents, compounds were extracted from the selected gel fractions using 20 mL ethyl acetate 3 times. The extracts from a single fraction were combined and ethyl acetate was evaporated under reduced pressure.
Molecular formulas of products were confirmed by UPLC-qTOF-MS/MS analysis. Identification of compounds was performed as described previously, with slight modifications [56], on the Acquity ultra-performance liquid chromatography (UPLC) system, coupled with a quadrupole-time of flight (q-TOF) MS instrument (UPLC/Synapt q-TOF MS, Waters Corp., Milford, MA, USA), with an electrospray ionization (ESI) source. The separation was achieved on the Cadenza CD-C18 UP (75 mm × 2.0 mm i.d., 3 µm) column (Imtakt, Kyoto, Japan). The mobile phase was a mixture of 0.1% aqueous formic acid v/v (A) and acetonitrile (B). The gradient program was as follows: initial conditions-1% B in A, 12 min-25% B in A, 19 min-100% B, 20 min-1% B in A. The flow rate was 0.45 mL/min, and the injection volume was 5 µL. The column was operated at 30 • C. The major operating parameters for the q-TOF MS were set as follows: capillary voltage 2.0 kV, cone voltage 40 V, cone gas flow of 11 L/h, collision energy 28-30 eV, source temperature 100 • C, desolvation temperature 250 • C, collision gas, argon; desolvation gas (nitrogen) flow rate, 600 L/h; data acquisition range, m/z 100-2000 Da; ionization mode, negative and positive. The data were collected with Mass-Lynx V 4.1 software. The runs were monitored at a wavelength of 254 nm.

Screening Procedure
Experiments were carried out using Sabouraud medium (10 g aminobac, 30 g glucose, 1 L distilled water). The microorganism was transferred to a 300 mL Erlenmeyer flask with 100 mL medium. Pre-incubation was carried out on a rotary shaker (140 rpm) at 25 • C for 72 h. The screening was also performed in 300 mL Erlenmeyer flasks with 100 mL of Sabouraud liquid medium. The pre-grown culture (0.5 mL) was transferred to an Erlenmeyer flask, and after 72 h incubation, 10 mg of substrate 3 or 4, dissolved in 0.5 mL of dimethyl sulfoxide, was added. The molar concentrations of substrates 3 and 4 were 0.42 mM. The biotransformation was run under the same conditions as pre-incubation. After 3, 6 and 9 days of substrate incubation samples were collected and extracted once with 30 mL of ethyl acetate. The extracts were dried with anhydrous magnesium sulfate for 5 min, concentrated in vacuo and analyzed by TLC and HPLC methods. Stability of the substrate was evaluated under identical conditions, without using a biocatalyst. Control cultivation with no substrate has also been performed.

The Semi-Preparative Biotransformations
The semi-preparative biotransformations were performed in 2 L flasks with 500 mL of the Sabouraud medium (10 g aminobac, 30 g glucose, 1 L distilled water) each. The pre-incubation culture (1 mL) was transferred to the flask and incubated for 72 h same as during the screening procedure. Afterwards, 50 mg of substrate 3 or 4, dissolved in 2.5 mL of dimethyl sulfoxide, was added and the cultures were shaken on a rotary shaker for 10 days. The molar concentrations of substrates 3 and 4 were 0.42 mm. After the confirmation of complete substrate conversion (or lack of further substrate conversion) metabolites were extracted 2 times using each time 300 mL of ethyl acetate. The combined extracts were dried with anhydrous magnesium sulfate for 5 min and then concentrated using a rotary evaporator. Biotransformation products were separated using preparative TLC plates and analyzed by NMR and UPLC-qTOF-MS/MS.

Conclusions
In this paper we present the results of the glycosylation of a flavanone and chalcone with methyl moiety in entomopathogenic filamentous fungi cultures. The strain B. bassiana KCH J1.5 was able to hydroxylate methyl moiety at C-6 of 6-methylflavanone and also to attach to the glycosyl moiety at this site. These microbial transformations have not yet been described in the scientific literature. Moreover, B. bassiana KCH J1.5 glycosylated 2 -hydroxyl-5 -methylchalcone at C-3 and 6-methylflavanone at C-3 . The B. bassiana KCH J1.5 enzymatic system also catalyzed hydroxylation of the flavanone skeleton at C-4 . The second utilized strain-I. fumosorosea KCH J2-glycosylated 2 -hydroxyl-5 -methylchalcone at C-3 and 6-methylflavanone at C-4 and C-4. It can be assumed that attachment of the glycosyl moiety at C-4 was preceded by the carbonyl group reduction. This strain was not able to hydroxylate the methyl moiety. All biotransformation products have not been previously described in the scientific literature and may be used in studies assessing their biological activity and bioavailability.