Blinatumomab and Inotuzumab Ozogamicin Sequential Use for the Treatment of Relapsed/Refractory Acute Lymphoblastic Leukemia: A Real-Life Campus All Study

Simple Summary Immunotherapy has improved the outcome of relapsed/refractory B-lymphoblastic leukemia. However, little is known about the outcome after recurrence and re-treatment with monoclonal antibodies. The aim of our retrospective study was to evaluate the efficacy and safety of blinatumomab and inotuzumab ozogamicin used for different disease relapses. This multicenter experience of the Campus ALL Italian study group described 71 patients with relapsed/refractory B-lymphoblastic leukemia treated with both blinatumomab and inotuzumab ozogamicin in any sequence. The sequential immunotherapy strategy demonstrated feasibility and efficacy in terms of minimal residual disease, overall and disease-free survival, and as a bridge to allotransplantation. Abstract Background: Blinatumomab (Blina) and inotuzumab ozogamicin (InO) has improved the outcome of relapsed/refractory B-lymphoblastic leukemia (R/R B-ALL). However, little is known about the outcome after recurrence and re-treatment with immunotherapy. Methods: We describe 71 R/R B-ALL patients treated for different relapses with Blina and InO. Blina was the first treatment in 57 patients and InO in 14. Twenty-seven patients had a previous allogeneic hematopoietic stem cell transplantation (allo-HSCT). Results: In the Blina/InO group, after Blina, 36 patients (63%) achieved a complete remission (CR), with 42% of negative minimal residual disease (MRD−); after InO, a CR was achieved in 47 patients (82%, 34 MRD−). In the InO/Blina group, after InO, 13 cases (93%) reached a CR (6 MRD−); after Blina, a CR was re-achieved in 6 cases (43%, 3 MRD−). Twenty-six patients proceeded to allo-HSCT. In the Blina/InO group, the median overall survival (OS) was 19 months; the disease-free survival (DFS) after Blina was 7.4 months (11.6 vs. 2.7 months in MRD− vs. MRD+, p = 0.03) and after InO, 5.4 months. In the InO/Blina group, the median OS was 9.4 months; the median DFS after InO was 5.1 months and 1.5 months after Blina (8.7 vs. 2.5 months in MRD− vs. MRD+, p = 0.02). With a median follow-up of 16.5 months from the start of immunotherapy, 24 patients (34%) are alive and 16 (22%) are alive in CR. Conclusion: In our series of R/R B-ALL, Blina and InO treatment demonstrate efficacy for subsequent relapses in terms of MRD response, OS and DFS, and as a bridge to allo-HSCT.

CAR-T cell is the most recent immunotherapy that has become available in this setting, demonstrating high efficacy.A recent large meta-analysis analyzed the results of 38 reports, which enrolled 2134 patients.An overall response rate of 76% was reported, while median OS and event-free survival (EFS) were 36.2 months and 13.3 months, respectively [13].
The study explored many open questions, as key modulators of response, including costimulatory domains, disease status, age, and lymphodepletion.Costimulatory domain 4-1BB in the CAR construct, low-dose cyclophosphamide lymphodepletion, and pretreatment morphologic complete remission seem to be associated with better OS, and morphologic remission and 4-1BB domain are also associated with better EFS.These findings confirm that CAR-T cell therapy may be associated with long-term benefits in R/R B-ALL patients.However, more studies are needed to understand the role of numerous Cancers 2023, 15, 4623 3 of 13 variables on the outcome of CAR-T cell therapy.Among these are the selection criteria of the patients eligible, the best type of construct to use, the type of lymphodepletion regimen, and most importantly, the long-term efficacy and the indication of HSCT consolidation [13].Furthermore, a direct comparison of CAR-T cells with Blina and InO therapies is lacking.
Blina is a CD3/CD19-targeting bispecific T-cell engager that recruits CD3 + effector T cells to kill CD19 + ALL blasts.In the phase III TOWER trial, the rate of CR or CR with incomplete hematologic recovery (CRi) was significantly greater for R/R B-ALL patients treated with Blina compared to patients who received standard-of-care chemotherapy (44% vs. 25%).Of the 271 patients treated with Blina, 24% proceeded to allo-HSCT, with 10% of the patients requiring pre-transplant salvage treatment.After 12 months, the median OS was significantly higher in the Blina group than in the chemotherapy group (8 vs. 4 months) [4].Blina demonstrated efficacy in monotherapy also in Philadelphia (Ph) positive R/R ALL patients.In the ALCANTARA study [6], 45 patients previously treated with two or more tyrosine kinase inhibitors (TKI) were reported.Sixteen patients obtained CR/CRi after two cycles of Blina.MRD negativity was achieved in 12/14 CR patients.An incidence CRS, neutropenia, and thrombocytopenia of >3 grade occurred in 82% of the patients.
InO is a CD22-directed humanized monoclonal antibody conjugated to calicheamicin.In the phase III INO-VATE trial, in R/R B-ALL patients, InO demonstrated to be superior to the standard of care, with a CR/CRi in 74% versus 31% [5].Patients in the InO group proceeded to an allo-HSCT in 40% of the cases, without a need for an additional salvage line, compared to 11% of the patients undergoing standard-of-care treatment.The median OS was 7.7 months in the InO group compared to 6.2 months in the standard-of-care group, and the 2-year OS rate was 23% versus 10%, respectively [5].
Nevertheless, although Blina and InO have improved the prognosis of both Phnegative and Ph-positive R/R B-ALL patients, little is known on the outcome of patients after the recurrence of their disease treatment with the two monoclonal antibodies sequentially.We hereby describe the clinical characteristics and outcome of 71 patients with R/R B-ALL treated with both Blina and InO in any sequence-Blina/InO or InO/Blina-for different disease recurrences, in the context of a multicenter study of the Campus ALL Italian study group.

Patients and Methods
We conducted a multicenter, retrospective analysis of 71 adult patients with R/R B-ALL treated with Blina and InO in any sequence (Blina/InO or InO/Blina) between March 2013 and February 2020.All procedures were in accordance with the ethical standards of the responsible Ethical Committee on human experimentation (institutional and national) and with the Helsinki Declaration of 1975, as revised in 2013.In each patient, Blina or InO were used for different disease recurrences.Between the two immunotherapies, other treatments could be administered.
The choice of using Blina or InO first relies on the clinical judgement of the single treating physician.
Blina is approved in Italy for hematologic relapse/resistance and for a blast count of <5% (minimal residual disease, MRD, and persistence), while InO is approved only for hematologic relapse/resistance.Nevertheless, in our study, InO was used in a minority of patients also with a BM blast count of <5%, on the basis of individual assessment and as an off-label prescription.
Patients were grouped according to the antibody that was used first: Blina/InO and InO/Blina groups.Medical records were reviewed to collect demographic, patient-related, disease-related, and clinical outcome data.The expression of CD19 and CD22 were measured by flow cytometry.Response evaluation was performed by a cytological examination of bone marrow (BM) smears.Flow cytometry and polymerase chain reaction (PCR) of leukemia-specific rearrangements of immunoglobulin genes or BCR::ABL1 transcript levels were used to monitor MRD.CR was defined if a patient had <5% blasts in BM smears and an absence of extramedullary manifestations; a negative MRD response was defined as <0.01%(10 −4 ) leukemic cells in the BM.OS was defined as the time from the start of the first immunotherapy agent to death from any cause or last contact, and disease-free survival (DFS) was defined as the time to relapse.
For the first cycle, Blina was administered by continuous intravenous infusion with a dose of 9 µg/day over 24 h for the first week and 28 µg/day over 24 h for the remaining 3 weeks, followed by a 2-week break.The following cycles were conducted with a dose of 28 µg/day from the beginning.InO was given intravenously at a dose of 1.8 mg/m 2 for the first cycle and then 1.5 mg/m 2 , divided in three weekly doses.Cycle 1 usually lasted 21 days, and the subsequent cycles were 28 days each.The demographic and disease characteristics are summarized with descriptive statistics.Averaged data were expressed as a median (range).A chi-square test was used to investigate correlations between all the nominal variables described.The Mann-Whitney correlation test was used to compare continuous variables between groups.Survival curves were generated using the Kaplan-Meier method and were compared between groups via the log-rank test.Values of p < 0.05 were considered significant; p ns was used to indicate a statistical non-significance.All statistical analyses were performed using STAT VIEW SAS V. 5.0.
An alluvial diagram was elaborated with the RAWGraphs 2.0 software.

Baselines Characteristics
Seventy-one patients from 19 Italian hematologic centers participating in the Campus ALL network were identified.
The treatment flow is reported in Figure 1.
(PCR) of leukemia-specific rearrangements of immunoglobulin genes or BCR::ABL1 tran script levels were used to monitor MRD.CR was defined if a patient had <5% blasts in BM smears and an absence of extramedullary manifestations; a negative MRD response wa defined as <0.01%(10 −4 ) leukemic cells in the BM.OS was defined as the time from th start of the first immunotherapy agent to death from any cause or last contact, and diseas free survival (DFS) was defined as the time to relapse.For the first cycle, Blina was administered by continuous intravenous infusion wit a dose of 9 µg/day over 24 h for the first week and 28 µg/day over 24 h for the remainin 3 weeks, followed by a 2-week break.The following cycles were conducted with a dose o 28 µg/day from the beginning.InO was given intravenously at a dose of 1.8 mg/m 2 for th first cycle and then 1.5 mg/m 2 , divided in three weekly doses.Cycle 1 usually lasted 2 days, and the subsequent cycles were 28 days each.The demographic and disease chara teristics are summarized with descriptive statistics.Averaged data were expressed as median (range).A chi-square test was used to investigate correlations between all th nominal variables described.The Mann-Whitney correlation test was used to compar continuous variables between groups.Survival curves were generated using the Kaplan Meier method and were compared between groups via the log-rank test.Values of p < 0.0 were considered significant; p ns was used to indicate a statistical non-significance.A statistical analyses were performed using STAT VIEW SAS V. 5.0.
An alluvial diagram was elaborated with the RAWGraphs 2.0 software.

Baselines characteristics
Seventy-one patients from 19 Italian hematologic centers participating in the Campu ALL network were identified.
The treatment flow is reported in Figure 1.Clinical characteristics are summarized in Table 1.Clinical characteristics are summarized in Table 1.
TKI therapy was associated with immunotherapy in two patients in the Blina/InO group (ponatinib) and in two patients in the InO/Blina group (ponatinib and bosutinib).
Forty patients received chemotherapy/TKI therapy between their exposures to the two immunotherapy agents (Blina/InO: 33/57, 58%; InO/Blina: 7/14, 50%, p not significant).Only two patients received CAR-T cell therapy.The low number of patients treated with CAR-T cells in our series is due to the fact that this immunotherapy was approved in Italy only 6 months before the end of the observational period of the present study.
The sequential therapy institutions and the response to these therapy and interim treatments between the immunotherapies are reported in Figures 1 and 2. In the Blina/InO group, the median number of treatments between the two immunotherapy agents was 1 (range 0-3), similar to the InO/Blina group (median number of treatments 1, range 0-2) (p ns).In the Blina/InO group, the median number of treatments between the two immunotherapy agents was 1 (range 0-3), similar to the InO/Blina group (median number of treatments 1, range 0-2) (p ns).
We also investigated the impact of leukemia bulk on the response during the Blina and InO therapies in the Blina/InO and InO/Blina groups and found that blasts of >50% were associated with a significantly worse CR rate after the Blina therapy, while no influence of the blast percentage was evidenced after the InO treatment.
In particular, in the Blina/InO group, CR was obtained in 45% of the patients with BM blasts of <50% vs. 13% of those with BM blasts of >50% (p = 0.04).
Similar results were obtained in the InO/Blina group after the Blina treatment (CR of 46% with BM blasts of <50% vs. 8% with BM blasts of >50%, p = 0.05).
No statistically significant differences in terms of CR were observed for the Ph+ and Ph-negative patients in both the Blina/InO and InO/Blina groups.
Immunotherapy was used as a bridge to allo-HSCT in 26 patients (37%) of whom 24 were in the Blina/InO population and 2 were in the InO/Blina group, and 14 of these were represented by a second HSCT.
The median OS from the first immunotherapy was longer in the Blina/InO cohort than in the InO/Blina group, but statistical significance was not reached (21.9 months vs. 15.3 months, respectively, p = ns, Figure 3).From the first immunotherapy, in the Blina/InO group, the median OS was 19 months and after InO, 6.3 months.The OS in the patients who reached an MRD negativity was not significantly different compared to that of the patients who remained MRD+.DFS after Blina was 7.4 months and was significantly better in MRD− compared to MRD+ patients (11.6 vs. 2.7 months, p = 0.03).After InO, DFS was 5.4 months, with no significant difference between the patients who became MRD− or did not.In the InO/Blina group, the median OS was 9.4 months and after Blina, 4.6 months, and it was significantly better in patients who obtained an MRD negativity (7.5 vs. 2.8 months, p = 0.02).In the InO/Blina group, the median DFS was 6.6 months (5.1 after the start of InO and 1.5 months after Blina), and it appears to be longer in patients who became MRD− after Blina (p = 0.02).Overall, the patients who reached negative MRD statues after the second-line immunotherapy witnessed a significantly better OS (p = 0.001).Interestingly, the OS and DFS from the first immunotherapy were longer in the group of patients who had undergone an allo-HSCT (data available for 64/71 patients) prior to starting the immunotherapy: the median OS was 24.2 vs. 13 months (p = 0.022).Allo-HSCT after the second immunotherapy was associated with a better OS and DFS, which was not significant (median OS, 9.8 months and median DFS, 7.2 months vs. 7.8 and 4.4 months).
With a median follow-up time of 16.5 months from the start of the immunotherapy and 33.8 months from the initial diagnosis, 24 patients (34%) are still alive and 16 (22%) are alive in CR.In the Blina/InO group, 24% of the patients are alive and in CR, while in the InO/Blina cohort, three patients (21%) are still alive and in CR.
No statistically significant differences in terms of DFS and OS were observed for the Ph-negative and Ph+ patients in both the Blina/InO and InO/Blina groups.
The cause of death was attributed to ALL progression in 34 patients (48%), infection in 6 patients (8%), allo-HSCT-related complications in 6 (8%), and bleeding complications in 1 case.Five patients (7%) died in CR due to a veno-occlusive disease during allo-HSCT after InO salvage treatment.Even if the study was not designed to evaluate the incidence of VOD and its possible correlation with a cumulative InO dose, in our series, the patients who developed a fatal VOD presented a total dose of InO (median number of cycles 2, range 1-2) comparable to the other patients who received allo-HSCT (median number of cycles 2, range 1-4) without the occurrence of this fatal complication.
For the InO/Blina group, exposure to InO was associated with grade > 3 AEs in three patients (21%), with one case (7%) of hematologic toxicity and two cases (14%) of extra-hematologic AEs (one liver function impairment).Infectious complications occurred in four cases (28%).The Blina treatment was associated with grade > 3 AEs in three patients (21%), with one case (7%) of hematologic toxicity and two cases (14%) of extra-hematologic AEs (one pulmonary thromboembolism and one deep vein thrombosis).Infectious complications occurred in four cases (28%).

Discussion
The landscape of the therapeutic approach to R/R B-ALL has rapidly evolved in recent times, given the availability of potent immunotherapies such as Blina, InO, and more recently, CAR-T cells.
Furthermore, these agents, in particular Blina and InO, are more and more used in association and are sequenced with chemotherapy and TKIs, with encouraging results in terms of an increasing OS and DFS [12].
In this scenario, CAR-T cell therapy was recently introduced in the therapeutic armamentarium of R/R B-ALL, with very promising outcomes [13,[19][20][21].
In particular, CAR-T cell therapy was approved in Italy in August 2019, a few months before the end of the enrollment period of our study, and therefore, it did not have a significant impact on the treatment choice of our series of patients.
Nevertheless, with its wider use in the immediate future, several clinical questions will emerge, among which concern the effective long-term benefit, the best CAR-T construct and cellular source, the most efficacious lymphodepletion regimens, and the best sequence with HSCT and their use in first line [13,[19][20][21].Following the expansion of their application, appropriate guidelines will be needed in order to assign patients to the best treatment choice and timing among Blina, InO, and CAR-T cells.
In particular, Blina maintenance was investigated in a randomized, phase 3 study of Blina vs. SOC in adults with R/R Ph-negative B-ALL, and this demonstrated that patients achieving remission with Blina had a longer OS with maintenance Blina therapy [22].Moreover, both Blina and InO have been used in MRD-positive patients: Blina has demonstrated efficacy in eradicating MRD in Ph-negative B-ALL in the BLAST study [15], and InO is currently being investigated in this setting both in Ph-negative and Ph+ B-ALL patients who are candidates of HSCT (GIMEMA ALL2418, NCT03610438) [8].
As a consequence of the promising results observed, these studies have prompted the exploration of further innovative approaches with Blina and InO in association and/or sequencing in the attempt to enhance their efficacy.
From this perspective, as tumor bulk has been demonstrated to have a significant impact on Blina therapy outcomes, an ongoing trial is investigating the role of InO to decrease disease burdens, followed by Blina to maintain remission (NCT03739814) in B-ALL R/R patients or in newly diagnosed B-ALL elderly patients.This approach is particularly interesting for InO's ability to induce deep and rapid responses, potentially optimizing the subsequent Blina treatment and at the same time, appropriately distancing HSCT from InO treatment, reducing, in this way, the InO risk of VOD.
As a last point, the association of InO, with or without Blina, with a reduced intensity chemotherapy (mini-Hyper-CVD) was successfully used as salvage therapy in Ph-negative B-ALL patients [11], with a significant enhancement in efficacy, maintaining a manageable toxicity, especially after reducing the chemotherapy intensity.With this approach, younger patients had overall and complete responses of 83% and 63%, with a high MRD negativity (82% of responders).Hepatic sinusoidal obstruction syndrome decreased significantly from 13% to 2% after reducing the regimen intensity.With a median follow up of 48 months, the median OS was 17 months and at 3 years, the OS was 34% with mini-Hyper-CVD plus InO and 52% with additional Blina.Interestingly, HSCT did not improve the OS [11], suggesting that its role after the introduction of these combined treatments should be further investigated and may change in the future.
Blina and InO have therefore become the standard of care for the treatment of R/R, and Blina has also become the standard of care for the eradication of persistent MRD.
These studies anticipated the introduction of these agents also in the first-line treatment of Ph+ and Ph-negative B-ALL patients in different combinations and sequences with a TKI and/or chemotherapy.
In the Ph+ ALL patients, the most interesting strategy is represented by the attempt to use a chemo-free induction regimen approach, adding a TKI to the Blina treatment.In particular, Blina plus dasatinib or ponatinib used in the front line, demonstrated optimal outcomes in terms of response rates, OS, and DFS [23][24][25].In addition, this approach is currently being investigated in a randomized phase 3 study that compares upfront Blina plus ponatinib treatment versus the standard regimen, represented by intensive chemotherapy plus imatinib (GIMEMA ALL2820, NCT04722848).
On the other hand, in the Ph-negative patients, Blina has been added in the first line to standard chemotherapy (GIMEMA LAL2317, NCT03367299) [26] and has also been associated with InO and modified mini-Hyper-CVD [27], in this case also demonstrating manageable toxicity and high efficacy.
Lastly, Blina has also been introduced as a consolidation regimen after first line treatment, reporting promising results [28].
Nevertheless, few data are available on the prognosis of R/R B-ALL salvaged with Blina or InO after a previous first line of immunotherapy.
In our multicenter, retrospective Campus ALL study, we evaluated R/R B-ALL patients in which Blina and InO were used for different disease recurrences.There are few published analyses on adult R/R B-ALL patients who received both Blina and InO treatments.Recently, Badar et al. reported a subset analysis on 61 out of a total of 276 patients treated with immunotherapy, who received Blina and InO for different relapses [29].
The sequence of Blina and InO was administered to 57 cases, while the InO and Blina sequence was used in the remaining 14 patients.The baseline characteristics were comparable.Forty patients received very etherogeneous additional treatments between the two immunotherapies.Even if this may have introduced possible biases in the outcomes, nevertheless, the global frequency of all these treatments were comparable in the two groups.
The median number of cycles for the second agent was 1 in the InO group (range 1-6) and 1 in the Blina group (range 1-4).The CR/CRi rate was 58% or 52% for the patients who received InO or Blina, respectively, as the second immunotherapeutic drug.
CR is significantly better in patients starting the Blina treatment with <50% BM blasts, confirming the reported relevance of debulking treatment prior to Blina delivery, in order to optimize its efficacy, while no impact of the blast number seems to be evident for the InO therapy [30,31].
Historically, the response rate in patients with R/R B-ALL has been reported to be inferior in subsequent lines of chemotherapy, from approximately 40% in first salvage to 20% after the second/third salvage regimens [1][2][3].Differently from conventional therapy, we have observed higher CR rates, similar to those reported in the seminal trials using Blina and InO in the setting of R/R B-ALL patients [4,5].Similar to Badar's study, more than half of our patients relapsed or progressed after the first-line immunotherapy was salvaged with the use of the second immunotherapeutic agent [29].In our Blina/InO group, the rate of CR after Blina was 63%, and after InO, it increased to 82%, with 60% of the patients obtaining a negative MRD.The InO/Blina group was also characterized by a high CR rate and a significant possibility of reaching MRD negativity, also after the second immunotherapy.As in the analysis by Badar et al., the median OS appeared to be longer, even if not significantly, for patients who received InO as the second immunotherapeutic agent [29].
The TOWER and INO-VATE clinical trials also suggested that Blina and InO are successful salvage options for patients with R/R B-ALL and bridge them to an allo-HSCT to obtain a long-term cure [4,5], and recent studies suggest that a combined approach will be the winning strategy to optimize the response to these drugs.
In our report, approximately 40% of the patients were successfully bridged to allo-HSCT and had the possibility of taking advantage of the described benefit of allo-HSCT after immunotherapy in improving the duration of the response and OS [9,12].In our study, allo-HSCT was associated with a longer survival duration, albeit not reaching statistical significance.
Interestingly, we observed that OS and DFS from the first immunotherapy were superior in patients who had relapsed after a previous allo-HSCT, possibly due to a role of CD3 lymphocye of the donor, which may be more efficient as immunologic effectors once engaged by Blina.
When selecting immunotherapy (Blina or InO), potential AEs are one of the most important considerations in order to select the optimal treatment, considering both patient-related and disease-specific needs.The toxicity profile of Blina mainly consists of the cytokine release syndrome and of neurologic symptoms, while InO targets the liver with sinusoidal obstruction syndrome, in particular after HSCT [4][5][6][7][8]10,11].In our study, about 25% of the patients experienced ≥ G3 AEs, not significantly different in the Blina/InO and InO/Blina groups and comparable to those observed in the TOWER and INO-VATE trials [4,5].The cause of death was mainly attributed to ALL's progression, infection, and allo-HSCT-related complications (8%, mainly due to veno-occlusive disease).

Conclusions
The approval of novel immunotherapeutic agents has markedly impacted the outcome of R/R B-ALL patients.The treatment choice requires that multiple factors are considered, including both patient-related and disease-specific characteristics.
In the present study, we described a very difficult clinical scenario, regarding those patients experiencing subsequent relapses and those who have been treated with both Blina and InO in any sequence, also with intercurrent salvage therapies between the two immunotherapies.This excludes all the patients who were cured after treatment with only Blina or InO.
Nevertheless, the clinical outcomes of these patients are surprisingly similar to those reported in patients receiving only either one of the two agents, underlining the favorable clinical impact of Blina and InO applied even in what may be considered the worst scenario for R/R B-ALL patients, giving a strong rationale for their sequential use also in those patients who obtain a profound CR after the application of the first one.One possibility would be to use inotuzumab as the first agent in order to obtain an efficient debulking/CR and blinatumomab in the consolidation phase, with two rationales: distancing the InO treatment before eventual HSCT, therefore reducing the described risk of VOD and favoring patients' immune reconstitution, possibly improving the efficiency of CD3 lymphocyte immunological effectors engaged by Blina.
Our real-life multicenter study on R/R B-lineage ALL patients with multiple previous lines of treatment demonstrates the feasibility and efficacy of a sequential immunotherapy strategy in terms of MRD response, DFS, and OS, and as an effective bridge to allo-HSCT to treat subsequent relapses.Future studies are required to determine how to best combine and sequence these agents to achieve the best outcomes in R/R B-lineage ALL patients in order to reduce the incidence of relapse.Informed Consent Statement: Informed consent was obtained from all the subjects involved in this study.

Figure 1 .
Figure 1.Diagram of the treatment flow of the Blina/InO and InO/Blina groups.

Figure 1 .
Figure 1.Diagram of the treatment flow of the Blina/InO and InO/Blina groups.

14 Figure 2 .
Figure 2.This figure reports the response after InO or after Blina in the IB and BI groups: CR patients cluster in the light green bars, and no CR patients cluster in the yellow bars.In particular, for the response post InO, the patients that obtained a CR (clustering in the light green bar) are represented in light grey, cyan, pink (1 patient), violet (1 patient), and dijon (1 patient) and those who did not obtain CR (clustering in the yellow bars) are represented in light grey, dark grey (1 patient), pink (1 patient), and brown (1 patient).IB: InO/Blina group, BI: Blina/InO group, CR: Complete remission, CLOFA: clofarabine, CTX: cyclophosphamide, Tx: therapy.

Figure 2 .
Figure 2.This figure reports the response after InO or after Blina in the IB and BI groups: CR patients cluster in the light green bars, and no CR patients cluster in the yellow bars.In particular, for the response post InO, the patients that obtained a CR (clustering in the light green bar) are represented in light grey, cyan, pink (1 patient), violet (1 patient), and dijon (1 patient) and those who did not obtain CR (clustering in the yellow bars) are represented in light grey, dark grey (1 patient), pink (1 patient), and brown (1 patient).IB: InO/Blina group, BI: Blina/InO group, CR: Complete remission, CLOFA: clofarabine, CTX: cyclophosphamide, Tx: therapy.

Table 1 .
Clinical characteristics and toxicities of Blina/InO and InO/Blina groups at the start of the first and second immunotherapy.ECOG was reported at the start of the first immunotherapy.No significant differences between groups, except for age (* p = 0.001).