In Vivo Application of CRISPR/Cas9 Revealed Implication of Foxa1 and Foxp1 in Prostate Cancer Proliferation and Epithelial Plasticity

Simple Summary Prostate cancer is diagnosed in one out of eight men, with large implications on life quality. Forkhead box proteins are often found mutated in prostate cancer but their functions are still not fully understood. In this study, we applied CRISPR to investigate the function of two Forkhead box proteins, Foxa1 and Foxp1, in the mouse prostate in combination with loss of Pten. Our results reveal that Foxp1 is a tumor suppressor in prostate cancer progression by controlling proliferation and genes regulated by the androgen receptor. Foxa1 controls cell plasticity, as loss of Foxa1 converted the prostatic luminal cells to basal cells. Hereby, this study sheds light on two distinct functions of Forkhead box proteins in prostate cancer. Abstract Prostate cancer is the most common cancer in men in the Western world and the number is rising. Prostate cancer is notoriously heterogeneous, which makes it hard to generate and study in pre-clinical models. The family of Forkhead box (FOX) transcription factors are often altered in prostate cancer with especially high mutation burden in FOXA1 and FOXP1. FOXA1 harbors loss or gain of function mutations in 8% of prostate cancer, which increases to 14% in metastatic samples. FOXP1 predominately occurs with loss of function mutations in 7% of primary tumors, and similar incidents are found in metastatic samples. Here, we applied in vivo CRISPR editing, to study the loss of functions of these two FOX transcription factors, in murine prostate in combination with loss of Pten. Deficiency of Foxp1 increased proliferation in combination with loss of Pten. In contrast, proliferation was unchanged when androgen was deprived. The expression of Tmprss2 was increased when Foxp1 was mutated in vivo, showing that Foxp1 is a repressor for this androgen-regulated target. Furthermore, analysis of FOXP1 and TMPRSS2 expression in a human prostate cancer data set revealed a negative correlation. Mutation of Foxa1 in the murine prostate induces cell plasticity to luminal cells. Here, epithelial cells with loss of Foxa1 were transdifferentiated to cells with expression of the basal markers Ck5 and p63. Interestingly, these cells were located in the lumen and did not co-express Ck8. Overall, this study reveals that loss of Foxp1 increases cell proliferation, whereas loss of Foxa1 induces epithelial plasticity in prostate cancer.


Introduction
Prostate cancer (PCa) is the most common cancer in men in the Western world and the number is rising as life expectancy is increasing [1]. PCa is a slowly developing cancer and remains an indolent disease, but when the cancer has progressed to a metastatic stage, treatment options are limited. As a consequence, metastatic PCa has poor prognosis, with less than 30% overall survival after 5 years [1][2][3]. Targeting the androgen receptor (AR) by medical castration or target therapy results in a regression of the cancer, but

Cell Work and AAV Production
MEF cells from LSL-Cas9 mouse embryos have been described before. MEF and HEK293T cells were grown in DMEM (Sigma-Aldrich, St. Louis, MO, USA), supplemented with penicillin-streptomycin (Sigma-Aldrich) and 10% FCS (Gibco, Waltham, MA, USA) at 37 • C and 5% CO 2 . MEF cells were transfected with X-tremeGENE-9 according to the manufacturer's protocols and puromycine selections were applied for 48 h (2 µg/mL). AAV production was conducted in HEK293T cells as described before [22].

Virus Delivery to the Prostate and Castrations
Surgical delivery of AAV to the murine prostate was performed according to the previously established protocol [17]. In brief, 10-week-old mice were anesthetized with a mixture of medetomidin (40 mg/kg), midazolam (15 mg/kg), and burophanol (10 mg/kg), and an incision of 1.2 cm was made in the abdomen above the bladder. The seminal vesicles were lifted out and 20 µL virus (10 9 viral genomes) was injected into the anterior prostate lobe at each side. The tumor progression proceeded for 4 months, and the experiment was terminated afterwards. A subset of mice underwent castration 3 months post-operation and were left untreated for another month before the prostate was collected. For castration, the mice received anesthetization and an incision was made in the abdomen and the testes were lifted out. The blood supply was ligated and the testes were removed before the abdomen was closed.

DNA/RNA Isolation and PCR
DNA and RNA were isolated from the prostate tissue with the Qiagen AllPrep DNA/RNA kit according to the manufacturer's protocols (Qiagene, Hilden, Germany).  Table S1 for primers. Q-PCRs for target genes were performed on 20 ng total RNA from tissue samples with Brilliant III Ultra-Fast SYBR ® Green QPCR Master Mix (Agilent Technologies, Glostrup, Denmark) (see Table S1 for primer sequences). Data were analysed with the ∆∆CT method and normalized to Rpl32.

Statistics
GraphPad Prism was used for statistics analysis. An unpaired t-test was used for Ki67 quantification and analysis of gene expression. A p-value ≤ 0.05 was considered statistically significant between the two groups.

FOXA1 and FOXP1 Are Commonly Mutated in PCa
FOX genes have been reported to be among the most commonly mutated genes in PCa [23]. To assess if the mutation frequency was different in primary and metastatic PCa, two publicly available data sets were analysed. The mutation frequencies of FOXA1 and FOXP1 were assessed in primary PCa from the TCGA (Firehose legacy; https://www. cancer.gov/tcga, accessed on 5 February 2020) data set and revealed mutations in 8 and 7%, respectively ( Figure 1). In metastatic PCa, FOXA1 was found mutated in 14% of the samples, but for FOXP1 the number was not increased (SU2C/PCF [24]) ( Figure 1). Furthermore, the analysis indicated that the mutations in these two FOX genes were mutually exclusive in primary PCa but co-occurrent in metastatic disease (p = 0.029). Phosphatase and tensin homolog (PTEN) is another commonly mutated gene in PCa, and is a negative regulator of the PI3K pathway [20]. To assess the mutation frequency of PTEN in relation to FOXA1 and FOXP1, the same two data sets were analysed. PTEN was mutated in 22% of primary PCa, and this increased to 33% in metastatic samples ( Figure S1). The loss of PTEN in primary PCa co-occurred with a loss of FOXP1 (p = 0.028), but not with FOXA1 alterations. Interestingly, mutations in FOXA1 were predicted to be either gain or loss of function, indicating that FOXA1 can act as an oncogene or tumor suppressor in PCa. In contrast, mutations in FOXP1 and PTEN often resulted in loss of function ( Figure 1 and Figure  S1). These data revealed that FOXA1 and FOXP1 have distinct functions in PCa. FOXP1 is likely a tumor-suppressor gene in primary PCa, whereas the function of FOXA1 is context-specific.

DNA/RNA Isolation and PCR
DNA and RNA were isolated from the prostate tissue with the Qiagen AllPrep DNA/RNA kit according to the manufacturer's protocols (Qiagene, Hilden, Germany). PCR was performed on genomic DNA with Dream Taq master mix (Thermo Scientific, Waltham, MA, USA). See Table S1 for primers. Q-PCRs for target genes were performed on 20 ng total RNA from tissue samples with Brilliant III Ultra-Fast SYBR ® Green QPCR Master Mix (Agilent Technologies, Glostrup, Denmark) (see Table S1 for primer sequences). Data were analysed with the ΔΔCT method and normalized to Rpl32.

Statistics
GraphPad Prism was used for statistics analysis. An unpaired t-test was used for Ki67 quantification and analysis of gene expression. A p-value ≤ 0.05 was considered statistically significant between the two groups.

FOXA1 and FOXP1 Are Commonly Mutated in PCa
FOX genes have been reported to be among the most commonly mutated genes in PCa [23]. To assess if the mutation frequency was different in primary and metastatic PCa, two publicly available data sets were analysed. The mutation frequencies of FOXA1 and FOXP1 were assessed in primary PCa from the TCGA (Firehose legacy; https://www.cancer.gov/tcga, accessed on 5 February 2020) data set and revealed mutations in 8 and 7%, respectively ( Figure 1). In metastatic PCa, FOXA1 was found mutated in 14% of the samples, but for FOXP1 the number was not increased (SU2C/PCF [24]) ( Figure 1). Furthermore, the analysis indicated that the mutations in these two FOX genes were mutually exclusive in primary PCa but co-occurrent in metastatic disease (p = 0.029). Phosphatase and tensin homolog (PTEN) is another commonly mutated gene in PCa, and is a negative regulator of the PI3K pathway [20]. To assess the mutation frequency of PTEN in relation to FOXA1 and FOXP1, the same two data sets were analysed. PTEN was mutated in 22% of primary PCa, and this increased to 33% in metastatic samples ( Figure S1). The loss of PTEN in primary PCa co-occurred with a loss of FOXP1 (p = 0.028), but not with FOXA1 alterations. Interestingly, mutations in FOXA1 were predicted to be either gain or loss of function, indicating that FOXA1 can act as an oncogene or tumor suppressor in PCa. In contrast, mutations in FOXP1 and PTEN often resulted in loss of function (Figures 1 and S1). These data revealed that FOXA1 and FOXP1 have distinct functions in PCa. FOXP1 is likely a tumor-suppressor gene in primary PCa, whereas the function of FOXA1 is context-specific.

Loss of Foxp1 and Pten by CRISPR in the Murine Prostate
To investigate the function of Foxp1 in PCa initiation and progression in vivo, we applied CRISPR to generate loss of function. CRISPR guides for Foxp1 were designed to target the 5 end of the coding sequence to induce insertion or deletions (indel) in the reading frame. Guide RNAs for Foxp1 were cloned into a plasmid containing Cas9 and puromycin coding genes. The guide efficiency was validated in mouse embryonic fibroblasts (MEFs) and the guide with the highest efficiency was selected for further use ( Figure S2).
The selected guide RNA for Foxp1 was cloned into an AAV backbone together with a specific sgRNA for Pten ( Figure 2A). The AAV backbone also provided expression of Cre protein for activation of Cas9 and EGFP expression in transduced cells from transgene Cas9-EGFP mice [16]. AAVs were produced and MEF cells were transduced for a validation of the virus. Transduced MEF cells started to express EGFP due to Cre-mediated removal of a stop codon, confirming appropriated virus activation ( Figure 2B). DNA was isolated from the MEF cells and Sanger Sequencing was performed on the target regions of the two sgRNAs. The formation of indels was detected for both guides, confirming that the AAVs were capable of transducing cells and introducing indel formations at the designated targets ( Figure 2C).

Loss of Foxp1 Increases Androgen-Dependent Proliferation in the Mouse Prostate
The function of FOXP1 in PCa has been associated with tumor-suppressor functions through in vitro studies [10]. To assess the function in vivo, mice with loss of Foxp1 and Pten in the prostate epithelium were compared to Pten-deficient animals. IHC was performed on tissue sections for Foxp1 and p-Akt to identify areas with loss of both Pten and Foxp1. Hereafter, the tissue sections were stained for the proliferation marker Ki67, and positive cells were counted. Loss of Foxp1 in combination with Pten deficiency increases proliferation compared to the control with only loss of Pten ( Figure 3A,B). Foxp1 has been suggested to be a negative regulator of the AR transcriptional network. The expression of To induce PCa, ten-week-old Cas9 transgene mice were injected with AAV in the anterior prostate lobe. Loss of Foxp1/Shq1 in the mouse prostate has previously shown not to induce PCa [11]. Therefore, mice were injected with AAV particles that contain guide RNAs for Foxp1 and Pten, whereas control mice received AAV particles that only contained a guide for Pten. Four months post-virus delivery, the experiments were terminated and the prostates were isolated. The anterior lobes were enlarged in both groups of mice and areas with EGFP expression were visible ( Figure 2D). Next, the isolated prostate samples were analysed by histological alteration. H & E staining revealed areas of high-grade prostatic intraepithelial neoplasia (PIN) [25,26] in the anterior lobe and alteration in the stroma ( Figure 2E). These are all features related to loss of Pten and activation of PI3K in the prostatic epithelium. Immunohistochemistry (IHC) staining for phosphorylated Akt (p-Akt) confirmed increased levels in the transformed areas, and staining for Pten revealed loss of expression in both groups of mice ( Figure 2E and Figure S3). Staining for Foxp1 revealed loss of expression in the transduced area, whereas mice receiving only Pten virus showed strong expression of Foxp1. Staining for p63, a marker of basal cells, showed similar expression in both groups as was also seen for AR expression ( Figure 2E and Figure  S4). DNA was isolated from the prostatic tissues, and Sanger Sequencing was conducted to analyze the indels at the target genes. Pten was mutated in the control samples and in the mice that had been targeted by two guides. Analysis of Foxp1 alteration showed similar indel frequency as for Pten, indicating that loss of both genes co-occurs with the delivery of both guide RNAs ( Figure 2F). Overall, by in vivo application of CRISPR/Cas9 gene editing, Pten and Foxp1 were mutated in the mouse prostatic epithelium.

Loss of Foxp1 Increases Androgen-Dependent Proliferation in the Mouse Prostate
The function of FOXP1 in PCa has been associated with tumor-suppressor functions through in vitro studies [10]. To assess the function in vivo, mice with loss of Foxp1 and Pten in the prostate epithelium were compared to Pten-deficient animals. IHC was performed on tissue sections for Foxp1 and p-Akt to identify areas with loss of both Pten and Foxp1. Hereafter, the tissue sections were stained for the proliferation marker Ki67, and positive cells were counted. Loss of Foxp1 in combination with Pten deficiency increases proliferation compared to the control with only loss of Pten ( Figure 3A,B). Foxp1 has been suggested to be a negative regulator of the AR transcriptional network. The expression of ARregulated genes was assessed in the murine samples. Four genes, of which expression has been associated with AR, were assessed but only Tmprss2 was found to be affected. Tmprss2 expression was increased 3.5-fold when Foxp1 was lost, whereas Nkx3.1, App and Klk4 were unaltered ( Figure 3C). As androgen is a positive regulator of cell proliferation in the prostatic tissues, the increased proliferation in Foxp1-deficient tissues could be a consequence of increased AR activity. To assess the proliferation in absence of AR regulation, mice underwent castration 3 months after cancer initiation, and the prostatic tissues were analysed one month post-castration. Histological H & E and AR staining revealed that androgen had been abolished, and the samples were stained for Foxp1 and p-Akt to confirm CRISPR-induced mutations ( Figure 3D, Figure S4 and S5). Hereafter, sections were stained for Ki67 and the positive cells were counted from the two groups. The results showed that the increase of cell proliferation seen in Foxp1-deficient prostate was dismissed in the absence of testosterone ( Figure 3E). These data show that Foxp1 is a negative regulator of proliferation in the presence of testosterone in PCa. The in vivo analysis showed a negative correlation between Foxp1 expression and the AR-regulated gene Tmprss2. To evaluate if FOXP1 and TMPRSS2 are negatively correlated in human prostate cancer, TCGA data sets were analysed. The expressions of FOXP1 and TMPRSS2 are negatively correlated in prostate adenocarcinoma (TCGA, PanCancer Atlas; https: //www.cancer.gov/tcga, accessed on 9 May 2022).
However, expressions of NKX3-1, APP and KLK4 were not negatively correlated to FOXP1, similarly to the observations from our mouse model above ( Figure 3F). These analyses show a consistency between mice and humans concerning FOXP1 regulations of TMPRSS2 expression.
confirm CRISPR-induced mutations ( Figures 3D, S4 and S5). Hereafter, sections were stained for Ki67 and the positive cells were counted from the two groups. The results showed that the increase of cell proliferation seen in Foxp1-deficient prostate was dismissed in the absence of testosterone ( Figure 3E). These data show that Foxp1 is a negative regulator of proliferation in the presence of testosterone in PCa. The in vivo analysis showed a negative correlation between Foxp1 expression and the AR-regulated gene Tmprss2. To evaluate if FOXP1 and TMPRSS2 are negatively correlated in human prostate cancer, TCGA data sets were analysed. The expressions of FOXP1 and TMPRSS2 are negatively correlated in prostate adenocarcinoma (TCGA, PanCancer Atlas; https://www.cancer.gov/tcga, accessed on 9 May 2022).

Foxa1 Regulates Prostatic Cell Plasticity
FOXA1 is often altered in PCa as loss of function mutations or gain of function through amplifications. To assess the role of Foxa1 loss in PCa, we cloned a Foxa1 sgRNA for our AAV construct. Hereby, the construct contained sgRNAs for Pten, Foxa1 and Foxp1 and Cre expression ( Figure 4A). AAV particles were produced and MEF cells were transduced for validation of the virus. The MEF cells showed expression of EGFP, indicating Cre expression from the AAV and removal of the stop codon in front of Cas9 and EGFP ( Figure 4B). Indel analysis by Sanger Sequencing confirmed mutations induced by all three guides, guaranteeing the in vivo application of the virus ( Figure 4C). To assess the function of Foxa1 deficiency in combination with loss of Pten and Foxp1 in PCa, ten-week-old Cas9 transgene mice were injected with AAV in the anterior prostate lobe. Four months after, the prostates were collected for further analysis. The anterior lobe was enlarged for triple deficient mice but the sizes were similar to mice with only loss of Pten. Both groups of mice contained areas with EGFP expression in the prostatic lobes, confirming Cre recombination activities from the AAV ( Figure 4D). Histological analysis showed features of high-grade  Staining for Foxa1 and Foxp1 showed a loss of expression in triple deficient samples but a high level of expression in the Pten ∆P control ( Figure 4E). GFP positive biopsies were dissected for DNA and RNA extraction. Sanger Sequencing for CRISPR-induced mutations showed indel in all samples for Pten, Foxa1 and Foxp1 ( Figure 4F). Foxa1 has been shown to regulate AR target genes, as seen for Foxp1 [4,21]. Therefore, qPCR was performed for the expression of the four targets, Tmprss2, Nkx3.1, App and Klk4. Tmprss2 mRNA was increased as had been observed with the loss of Foxp1. However, App mRNA was also upregulated, indicating that Foxa1 is a possible negative regulator of this gene in vivo ( Figure 4G). The indel frequencies for Foxp1 and Pten were significantly increased, compared to the indel for Foxa1, even though the same samples were analysed ( Figure S6). Therefore, immunofluorescence staining for Foxa1 and p-Akt was performed, and it revealed cells positive for both Foxa1 and p-Akt. This shows sub-areas with loss of Pten, although without loss of Foxa1 in the altered prostatic tissues, suggesting a negative selection for loss of Foxa1 in this context ( Figure 5A,B).  Next, samples were stained for the basal cell marker p63, which showed normal basal layer at the stroma. Interestingly, triple deficient samples displayed p63 positive cells in the lumen. These were not seen in Pten ∆P control samples or samples deficient in Pten and Foxp1 (Figures 2E and 5C). To evaluate if loss of Foxa1 may induce basal cell marker expression, samples were co-stained for Foxa1 and the basal marker Ck5. Staining revealed that Ck5-expressing cells in the lumen were negative for Foxa1 ( Figure 5D). This indicates a negative correlation between Foxa1 and expression of basal cell markers. Ck5 positive cells in the lumen of the prostate have been shown to also express Ck8 and these cells have been marked as trans-amplifying cells [18,21]. To assess if loss of Foxa1 drives luminal cells into trans-amplifying cells, co-staining for Ck5 and Ck8 was conducted on tissue sections. Interestingly, Ck5-expressing cells did not co-express Ck8 in the prostatic lumen and no differences in trans-amplifying cells were observed ( Figure 5E,F). Overall, the loss of Foxa1 drives cell plasticity to induce basal cell expression in the luminal space of the prostatic lobe.

Discussion
In this study, we analysed the function of two Forkhead box protein functions in PCa initiation and progression in vivo. We applied CRISPR to generate loss of function of Foxa1 and Foxp1 in the murine prostate, in combination with the activated Pi3K pathway by simultaneous depletions of Pten. Our results show that Foxa1 and Foxp1 have distinct functions in PCa, as Foxa1 regulates cell plasticity and Foxp1 controls cell proliferation. Furthermore, both Fox proteins regulate specific gene expressions in vivo, which are known as AR targets. These findings are coherent with expression data from human PCa samples. Overall, we have shown that Foxa1 and Foxp1 are essential genes in prostate biology.
The implications of Fox proteins in prostate biology have been studied for many years. In particular, FOXA1 has been investigated thoroughly in prostate development [5] and in PCa, as this transcription factor commonly harbors genetic alterations in PCa [4,6]. Here, we analysed the mutation profile of FOXA1 in the TCGA data set containing more than 500 samples. This revealed that 8% of primary PCa shows alterations in FOXA1, as either loss or gain of function mutations. We focused on Foxa1 loss of function mutations in this study and revealed that Foxa1 is crucial for the cell identity. Loss of Foxa1 in the prostatic epithelium regulates cell plasticity, as the cells express basal cell markers even though the cells are located in the lumen of the prostatic lobe. The location of cells expressing basal markers in the lumen could involve invading of basal cells but we have not seen any evidence of this. Furthermore, immunofluorescence staining shows that Foxa1 expression is restricted to luminal cells in the prostatic lobe, indicating that Foxa1 is essential for luminal cell lineage. Foxa1 implication in cell identity has been associated with luminal cell expansion in organ culture studies and decrease of luminal cells in vivo [4,6,7]. This indicates that loss of Foxa1 is responsible for this change of cell identity, even though it has been studied in combination with loss of Foxp1 and Pten. Here our data show that loss of Foxa1 in adult prostate tissues differentiates the cells into basal-like cells with loss of luminal signatures. FOXA1 and FOXP1 have been shown to bind genomic regions containing overlapping AR binding sites and hereby synergistically regulate AR target genes in vitro [9,10,27]. We investigated the expression of four established AR targets, Nkx3.1, App, Klk4 and Tmprss2 in the murine prostate after loss of Foxp1. Interestingly, only Tmprss2 expression increased, whereas Nkx3.1, App and Klk4 expressions were unchanged. This shows that Foxp1 is working as a negative regulator on selected AR-regulated genes in vivo, and similar results were obtained from gene expression data sets of human PCa [11]. AR is known to increase cell survival and proliferation of the prostatic tissues, and we showed that loss of Foxp1 increased proliferation in the presence of testosterone together with an effect on Tmprss2 expression. This indicated that Foxp1 is a negative regulator of AR target genes that are responsible for proliferation. Consistent with our findings, others have shown upregulation of AR-regulated genes and increased proliferation when Foxp1 is depleted [10,11]. Future work will focus on Foxp1 molecular interaction on AR-regulated genes to shed light on the underlying mechanisms in PCa.
Loss of Foxa1 in combination with an abrogation of Foxp1 and Pten showed increased expression in App. This suggests that in vivo, Foxa1 is a negative regulator of App. Whether this dysregulation of App is directly controlled by Foxa1 or a combination of loss of both Fox genes has not been assessed. App has been shown to promote PCa growth and cell migration [27]. Hereby, Foxa1 can act as a tumor-suppressor gene in the prostatic epithelium, even though the majority of genetic alterations in FOXA1 are found as gain of function mutations [6]. Future work will apply CRISPR technology to study gain of function mutations of Foxa1 in the murine prostate.
In this study, we applied a CRISPR/Cas9 mouse model to investigate Foxa1 and Foxp1 in vivo. With this method, multiple genes can be simultaneously altered in the prostatic epithelial cells of the adult male mouse. Furthermore, tumorigenesis is initiated in single cells, which allows clonal expansion under natural selection, while empty vector or nontargeting sgRNA does not cause PCa formation [13,14]. Here, we observed that Foxa1 was found mutated to a lesser extent than Foxp1 and Pten. In contrast, indel formation in the MEF cells showed the same efficiency for Fox gene sgRNAs, whereas Pten had a decreased mutation rate. We have previously observed that the Pten sgRNA had a decreased efficiency in vitro, but was always found mutated in the transformed prostatic epithelium in vivo [14]. In this study, Pten was found lost in all PIN formations, showing a strong selection for mutations in this gene for the initiation of PCa formation. However, Foxa1 was absent in sub-areas of PIN formations, suggesting a negative selection for loss of Foxa1 in this context. This could be a biological selection, as loss of Foxa1 differentiated the luminal cells into basal cells. However, it could also reflect technical issues, as Foxa1 sgRNA was under the murine U6 promotor, whereas Pten and Foxp1 sgRNAs were controlled by human U6. Future work will investigate this possibility, but the in vitro data did not suggest differences in promotor activity, and other studies have also shown similarities between these Pol III promotors [28]. Overall, our CRISPR/Cas9 model for studying gene function in PCa provided new knowledge concerning the function of Foxa1 and Foxp1. The model allowed depletions of these factors in adult prostate epithelium in combination with a loss of Pten.

Conclusions
In this study, the functions of Foxa1 and Foxp1 in PCa have been assessed in vivo in combination with Pten mutation. By applying CRISPR/Cas9, mutations have been introduced to the adult mouse prostate and it was revealed that Foxp1 acts as a tumor-suppressor gene by controlling proliferation in an androgen-dependent manner. Molecular analysis revealed that Foxp1 is a negative regulator of a specific AR-regulated gene, Tmprss2, showing that Foxp1 is essential for prostate biology.
Depletion of Foxa1 in the context of Foxp1 and Pten mutations differentiated the prostatic epithelial cells to basal cells. This showed that Foxa1 is essential for prostate luminal cell identity. Furthermore, loss of Foxa1 also increased the expression of App, which is an AR-regulated gene, showing that Foxa1 can alter the expression of testosteroneregulated genes in the prostate. Overall, this study shows that two commonly mutated FOX genes in human PCa have distinct functions when studying in an in vivo setting, underlining the complexity of PCa initiation and progression.
Supplementary Materials: The following supporting information can be downloaded at: https: //www.mdpi.com/article/10.3390/cancers14184381/s1, Figure S1: Loss of PTEN co-occurrence with loss of FOXP1 in primary prostate adenocarcinoma; Figure S2: Guide efficiency for Foxa1 and Foxp1; Figure S3: Normal localization of the androgen receptor in the prostatic cells; Figure S4: Loss of Pten in the prostatic tissues; Figure S5: Castrated prostatic tissues contained loss of Foxp1 and increases p-Akt in Pten; Foxp1 deficient mice; Figure S6: Decreased mutations of Foxa1 compared to Pten and Foxp1; Table S1: Oligoes.