The Epithelial-to-Mesenchymal Transition in Cancer

The epithelial-to-mesenchymal transition (EMT) occurs during normal embryonic development, tissue regeneration, organ fibrosis, and wound healing.[...].

is highly permissive for phenotypic plasticity. Current knowledge on EOC heterogeneity in an EMT context is highlighted. Computational modeling of EMT is also presented. This review is followed by a description of EMT in non-small cell lung cancer (NSCLC) by Legras et al.-a cancer that is the major cause of cancer-related death in developed countries [10]. The authors report the role of the transcription factor TWIST1 in EMT in cells with epidermal growth factor receptor (EGFR) mutation and its association with low disease-free survival in EGFR-mutated lung adenocarcinoma patients. A large part of this overview is dedicated to EMT regulation by microRNAs. Next, EMT is described by Gaianigo et al. in pancreatic cancer (PC), which has a poor prognosis due to metastatic dissemination in the very first stages of tumor development mainly attributed to EMT [11]. The focus of this review is the involvement of EMT in treatment resistance. Among EMT actors, the authors give special attention to mutated RAS and the nuclear transcription factor yes-associated protein 1 (YAP1), one of the main transducers of the Hippo pathway. The authors also develop the importance of the large fibrotic tumor microenvironment called desmoplasia, which contributes to PC advancement, chemoresistance, and reduced drug delivery. The role of inflammation, microbiota, and treatment resistance related to EMT are also discussed. The review by Vu et al. summarizes EMT in colorectal cancer (CRC) [12]. In addition to classical transcription factors, the authors describe deeper PROX1 homeobox and forkhead box (FOX) transcription factors, their signaling pathways, several novel EMT inducers in colorectal cancers including neuropilin-2 (NRP2, a receptor for cell guidance molecule and growth factors), and downregulation of several microRNAs. Furthermore, the role of the EMT in circulating tumor cells (CTCs) is also discussed with the possible use of the mesenchymal markers as potential biomarkers for metastasis.
The following review by Grelet et al. focuses on specific aspects of EMT in cancers and cell lines, the molecular mechanisms regulating the TGF-β-induced EMT, and their implications in tumor metastasis [13]. The authors discuss the recent reports emphasizing the regulatory functions of non-coding RNA (microRNAs and lncRNAs, piRNAs, snRNAs, snoRNAs, circRNAs). In the second part of their survey, the authors provide experimental evidence of change in the expression of several tRNAs during TGF-β treatment of the human lung cancer cell line A549.
The third series of reviews addresses more specific mechanisms involved in EMT regulation [14][15][16][17]. First, Roche et al. present epigenetic regulation of EMT mainly in lung cancer with recent data on EZH2 (enhancer of zeste 2 polycomb repressive complex 2 subunit) that behaves as an oncogene in lung cancer associated with gene repression [14]. EZH2 is the catalytic subunit of the PRC2 (polycomb group PcG), which methylates lysine 27 of histone H3, inducing a repressive transcriptional mark. The authors mention that EZH2 inhibitors are under development with clinical trials ongoing. Next, Fu et al. describe the role of the Polo-like kinase 1 (PLK1)-a serine/threonine kinase-in EMT and tumor invasion [15]. Its overexpression correlates with increased cellular proliferation and poor prognosis in cancer patients. To its canonical role in almost every stage of cell division and cytokinesis execution, modulation of DNA replication, and cell survival, overexpressed PLK1 orchestrates EMT and associated events (such as invasion and therapeutic resistance) in tumor cells via the MAPK pathway by directly binding and phosphorylating CRAF (a member of the Raf kinase family), and through AKT or FoxM1-dependent pathways. The therapeutic use of PLK1 inhibitors is discussed. In the following review, Blackwell et al. describe exosomes that are important mediators of intercellular signaling and EMT, with resultant transformation of cancer cells to a more aggressive phenotype [16]. By concentrating proteins or RNA, exosomes may transform nearby cells, as an explanation for the "field effect" phenomenon. In addition, cancer-derived exosomes exert pro-angiogenic effects on epithelial cells. Then, Lu et al. review another interesting aspect of EMT events: the gain of multiple pericyte markers by cells undergoing EMT-called the epithelial-to-pericyte transition (EPT), possibly mediated by the serum response factor (SRF) [17]. These mesenchymal cells phenotypically and functionally resemble pericytes. They are attracted to blood vessels via paracrine PDGF signaling and associate to blood vessels through N-cadherin. EPT cancer cells associate with and stabilize blood vessels to fuel tumor growth, and EPT may promote therapy resistance to antiangiogenic agents. The authors propose a model for EPT regulation.
Next, Forte et al. address concerns about 2D cellular models to study EMT and strongly recommend in vitro tissue-derived cell spheroid models [18]. These three-dimensional (3D) cell culture systems-whose phenotype has been shown to be strongly dependent on TGF-β-regulated EMT/MET processes-present the advantage of exposing cells to more physiological conditions in a microenvironment where cell-cell and cell-matrix interactions are present, and recapitulate in vitro the hypoxic micro-environment of tissue stem cell niches and their formation. The authors explore the mechanistic correspondence in vivo and the possible pharmacological perspectives.
The last review by Jia et al. illustrates the concept of "landscape" previously defined by Waddington in the epigenetic field [19]. Each cell phenotype is considered as an "attractor" which is characterized by a unique gene expression pattern, and determined by interactions between multiple molecular players buffered against environmental fluctuations. Here, the authors illustrate how phenotypic plasticity in cancer cells enables them to acquire hybrid phenotypes during EMT. The perspective of this review is intended to encourage the exchange of ideas between cancer biologists and physicists interested in exploring the physics of biology.
In summary, this special issue covers EMT in several types of cancer, and reviews different actors in EMT regulation with potential therapeutic applications. More work is needed to advance this complex field with the best available models.

Conflicts of Interest:
The authors declare no conflict of interest.