Next Article in Journal
Design of an FPGA-Based Fuzzy Feedback Controller for Closed-Loop FES in Knee Joint Model
Next Article in Special Issue
Design and Fabrication of Organ-on-Chips: Promises and Challenges
Previous Article in Journal
A Prototype Sensor System Using Fabricated Piezoelectric Braided Cord for Work-Environment Measurement during Work from Home
Previous Article in Special Issue
Fabrication of Stromal Cell-Derived Factor-1 Contained in Gelatin/Hyaluronate Copolymer Mixed with Hydroxyapatite for Use in Traumatic Bone Defects
Article

A Personalized Glomerulus Chip Engineered from Stem Cell-Derived Epithelium and Vascular Endothelium

1
Department of Biomedical Engineering, Pratt School of Engineering, Duke University, Durham, NC 27708, USA
2
Center for Biomolecular and Tissue Engineering, Duke University, Durham, NC 27708, USA
3
Division of Nephrology, Department of Medicine, Duke University School of Medicine, Durham, NC 27708, USA
4
Department of Cell Biology, Duke University, Durham, NC 27708, USA
*
Author to whom correspondence should be addressed.
These authors contributed equally.
Academic Editors: Nur Mustafaoglu, Vasif Hasirci, Ken Takahashi and Menekse Ermis
Micromachines 2021, 12(8), 967; https://doi.org/10.3390/mi12080967
Received: 24 June 2021 / Revised: 7 August 2021 / Accepted: 13 August 2021 / Published: 16 August 2021
(This article belongs to the Special Issue 3D In Vitro Tissue and Organ Models)
Progress in understanding kidney disease mechanisms and the development of targeted therapeutics have been limited by the lack of functional in vitro models that can closely recapitulate human physiological responses. Organ Chip (or organ-on-a-chip) microfluidic devices provide unique opportunities to overcome some of these challenges given their ability to model the structure and function of tissues and organs in vitro. Previously established organ chip models typically consist of heterogenous cell populations sourced from multiple donors, limiting their applications in patient-specific disease modeling and personalized medicine. In this study, we engineered a personalized glomerulus chip system reconstituted from human induced pluripotent stem (iPS) cell-derived vascular endothelial cells (ECs) and podocytes from a single patient. Our stem cell-derived kidney glomerulus chip successfully mimics the structure and some essential functions of the glomerular filtration barrier. We further modeled glomerular injury in our tissue chips by administering a clinically relevant dose of the chemotherapy drug Adriamycin. The drug disrupts the structural integrity of the endothelium and the podocyte tissue layers, leading to significant albuminuria as observed in patients with glomerulopathies. We anticipate that the personalized glomerulus chip model established in this report could help advance future studies of kidney disease mechanisms and the discovery of personalized therapies. Given the remarkable ability of human iPS cells to differentiate into almost any cell type, this work also provides a blueprint for the establishment of more personalized organ chip and ‘body-on-a-chip’ models in the future. View Full-Text
Keywords: human induced pluripotent stem cells; podocytes; endothelial cells; kidney glomerulus; disease models; organ-on-a-chip; glomerulus chip; stem cell technologies; microfluidics; personalized medicine human induced pluripotent stem cells; podocytes; endothelial cells; kidney glomerulus; disease models; organ-on-a-chip; glomerulus chip; stem cell technologies; microfluidics; personalized medicine
Show Figures

Figure 1

MDPI and ACS Style

Roye, Y.; Bhattacharya, R.; Mou, X.; Zhou, Y.; Burt, M.A.; Musah, S. A Personalized Glomerulus Chip Engineered from Stem Cell-Derived Epithelium and Vascular Endothelium. Micromachines 2021, 12, 967. https://doi.org/10.3390/mi12080967

AMA Style

Roye Y, Bhattacharya R, Mou X, Zhou Y, Burt MA, Musah S. A Personalized Glomerulus Chip Engineered from Stem Cell-Derived Epithelium and Vascular Endothelium. Micromachines. 2021; 12(8):967. https://doi.org/10.3390/mi12080967

Chicago/Turabian Style

Roye, Yasmin, Rohan Bhattacharya, Xingrui Mou, Yuhao Zhou, Morgan A. Burt, and Samira Musah. 2021. "A Personalized Glomerulus Chip Engineered from Stem Cell-Derived Epithelium and Vascular Endothelium" Micromachines 12, no. 8: 967. https://doi.org/10.3390/mi12080967

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop