A Promising Biomolecule Able to Degrade Neutrophil Extracellular Traps: CdcPDE, a Rattlesnake Phosphodiesterase

Neutrophil extracellular traps (NETs) are an important mechanism for defense against pathogens. Their overproduction can be harmful since excessive NET formation promotes inflammation and tissue damage in several diseases. Nucleases are capable to degrade NET on basis of their DNA hydrolysis activity, including the CdcPDE, a nuclease isolated from Crotalus durissus collilineatus snake venom. Here, we report a new finding about CdcPDE activity, demonstrating its efficiency in degrading cell-free DNA from NETs, being a potential candidate to assist in therapies targeting inflammatory diseases.

Venomous animal-derived toxins are known to display fabulous pharmacological properties, representing interesting lead compounds for the development of novel medicines. Indeed, some venom-derived drugs are already in the market, and several are under clinical trials [14]. The global pharmaceutical industry has benefited greatly from biodiversity-rich countries stimulating the bioprospection of novel biomolecules derived from venoms for novel drug design [15].
DNAses are potent agents capable of degrading NETs, being able to digest the DNA present in the mesh [16]. CdcPDE, a phosphodiesterase, was recently (2021) isolated from the Crotalus durissus collilineatus rattlesnake venom, and was completely characterized. Moreover, functional previous studies demonstrated that the CdcPDE was able to inhibit ADP-induced platelet aggregation and to cause a cytotoxic effect to human keratinocytes [17]. Knowing that CdcPDE is classified as a nuclease, able to degrade DNA (i.e., DNAse [18,19], this study aimed to verify if the rattlesnake-derived molecule could degrade cell-free DNA from NETs ( Figure 1). biodiversity-rich countries stimulating the bioprospection of novel biomolecules derived from venoms for novel drug design [15].
DNAses are potent agents capable of degrading NETs, being able to digest the DNA present in the mesh [16]. CdcPDE, a phosphodiesterase, was recently (2021) isolated from the Crotalus durissus collilineatus rattlesnake venom, and was completely characterized Moreover, functional previous studies demonstrated that the CdcPDE was able to inhibi ADP-induced platelet aggregation and to cause a cytotoxic effect to human keratinocyte [17]. Knowing that CdcPDE is classified as a nuclease, able to degrade DNA (i.e., DNAse [18,19], this study aimed to verify if the rattlesnake-derived molecule could degrade cell free DNA from NETs ( Figure 1).

Results and Discussion
The isolation and characterization of CdcPDE was performed in our previous study showing its low recovery (0.71%) [17]. CdcPDE at 20 µg/mL (0.2 mM) exhibited significantly lower levels of cell-free-DNA in comparison with undigested groups (Figure 2), indicating it ability of cell-free DNA degradation. Although the highest tested concentration (20 µg/mL inhibited ~38%, the amount of the used CdcPDE was 25-times lower than the DNAse contro (500 µg/mL). Unfortunately, we could not test CdcPDE using higher concentration due to it very low recovery, making a dose-response curse unfeasible. Indeed, to enable to carry out a dose-response curve, assays with inhibitors, and in vivo tests, heterologous expression wil be necessary.

Results and Discussion
The isolation and characterization of CdcPDE was performed in our previous study, showing its low recovery (0.71%) [17]. CdcPDE at 20 µg/mL (0.2 mM) exhibited significantly lower levels of cell-free-DNA in comparison with undigested groups (Figure 2), indicating its ability of cell-free DNA degradation. Although the highest tested concentration (20 µg/mL) inhibited~38%, the amount of the used CdcPDE was 25-times lower than the DNAse control (500 µg/mL). Unfortunately, we could not test CdcPDE using higher concentration due to its very low recovery, making a dose-response curse unfeasible. Indeed, to enable to carry out a dose-response curve, assays with inhibitors, and in vivo tests, heterologous expression will be necessary.
DNAses are potent agents capable of degrading NETs, being able to digest the DN present in the mesh [16]. CdcPDE, a phosphodiesterase, was recently (2021) isolated fr the Crotalus durissus collilineatus rattlesnake venom, and was completely characteriz Moreover, functional previous studies demonstrated that the CdcPDE was able to inh ADP-induced platelet aggregation and to cause a cytotoxic effect to human keratinocy [17]. Knowing that CdcPDE is classified as a nuclease, able to degrade DNA (i.e., DNA [18,19], this study aimed to verify if the rattlesnake-derived molecule could degrade c free DNA from NETs ( Figure 1).

Results and Discussion
The isolation and characterization of CdcPDE was performed in our previous stu showing its low recovery (0.71%) [17]. CdcPDE at 20 µg/mL (0.2 mM) exhibited significan lower levels of cell-free-DNA in comparison with undigested groups (Figure 2), indicating ability of cell-free DNA degradation. Although the highest tested concentration (20 µg/m inhibited ~38%, the amount of the used CdcPDE was 25-times lower than the DNAse cont (500 µg/mL). Unfortunately, we could not test CdcPDE using higher concentration due to very low recovery, making a dose-response curse unfeasible. Indeed, to enable to carry ou dose-response curve, assays with inhibitors, and in vivo tests, heterologous expression w be necessary.  Others substrates were tested in PDEs studies to characterize their enzymatic activity, such as bis (p-nitrophenyl) phosphate (0.4 mM [20], 1 mM [17] and 5 mM [21]), adenosine triphosphate (ATP, 0.05 mM), nicotinamide adenine dinucleotide (NAD, 0.05 mM), adenosine diphosphate (ADP, 0.05 mM), nicotinamide guanine dinucleotide (NGD, 0.05 mM), and adenosine monophosphate (AMP, 0.05 mM) [22], among others [18,19]. Although our study did not aim to characterize the enzymatic kinetics of CdcPDE, using λDNA as substrate, we observed that the amount of substrate used in the cited studies is much higher than in our study (6 × 10 −13 mM), and even though the hydrolysis was observed. Therefore, our study indicates that CdcPDE may present a high specificity/affinity for the used substrate.
Only a few studies report that snake venoms can present DNAse activity [23,24]. Sittenfeld and colleagues (1991) tested DNAse activity of Bothrops asper, B. godmani, B. schlegelii, B. lateralis, B. nasutus, C. durissus, and Lachesis muta venoms, through radial diffusion in gel, and observed that all of them demonstrated DNAse activity [23]. Sales and Santoro (2008) have tested DNAse activity in 28 Brazilian venoms belonging to Bothrops, Crotalus, Lachesis, and Micrurus genera, observing that B. brazili presented the highest DNAse activity [24]. In contrast, here we pioneer demonstrated the DNAse activity of an isolated phosphodiesterase from the C. d. collilineatus venom. Moreover, to the best of our knowledge, this is the first study to test a snake venom-derived PDE using cell-free DNA from NETs.
Knowing that immune mechanisms participate on pathogenesis of several inflammatory diseases [25], the impairment of NETs degradation may promote endothelial damage, organ dysfunction, inflammation, and autoimmunity [26,27]. Supporting that, few studies showed that exogenous DNAse can improve the outcome of some diseases, such as sepsis and COVID-19 [28,29]. Therefore, attenuation of neutrophil-induced effects (i.e., NETs) may be a potent target for controlling diseases characterized by an influx of granulocytes and their activation, as was here demonstrated by CdcPDE biomolecule.

Conclusions
Our communication supports the use of NETs' inhibitors by degrading DNA-free from NETs, as a strategy to ameliorate multi-organ damage during the clinical course of NETs-associated inflammatory diseases. Although further studies are needed, our study pioneering shows that a snake-venom derived PDE can degrade cell-free DNA, which may contribute to reduce the pathogenicity of inflammatory diseases.

Methods
CdcPDE was isolated from Crotalus durissus collilineatus in our previous study [17]. The DNAse activity was measured following the protocol of Colón and colleagues (2019) [29] with some modifications. Briefly, we diluted λDNA from the Quant-iT™ , and after this, samples were treated with CdcPDE (10 or 20 µg/mL), Pulmozyme™ (500 µg/mL, Roche, Basel, Switzerland) that was the positive control of DNAse activity, and PBS (Cat. 14190144, Corning Inc., Corning, NY, USA) that was the negative control of DNAse activity. On the same plate, we placed the samples, Pulmozyme, or PBS with RPMI medium without the λDNA solution, this well was used to correct the amount of DNA contained in the samples. We incubated the plate for 2 h at 37 • C and immediately after, we used the SYTOX Green™ (Cat. S7020, ThermoFisher Scientific, Waltham, MA, USA) to stain the remaining DNA in the wells. After incubation for 5 min protected from the light, we used the Flexstation 3 (Molecular Devices, San Jose, CA, USA) to read the fluorescence at 488 nm and excitation at 525 nm. Our results are shown as the percentage of fluorescence at each well. The average of the wells containing only medium and the λDNA solution was considered 100% fluorescence.