Recent Advances on Biological Activities and Structural Modifications of Dehydroabietic Acid

Abstract Dehydroabietic acid is a tricyclic diterpenoid resin acid isolated from rosin. Dehydroabietic acid and its derivatives showed lots of medical and agricultural bioactivities, such as anticancer, antibacterial, antiviral, antiulcer, insecticidal, and herbicidal activities. This review summarized the research advances on the structural modification and total synthesis of dehydroabietic acid and its derivatives from 2015 to 2021, and analyzed the biotransformation and structure-activity relationships in order to provide a reference for the development and utilization of dehydroabietic acid and its derivatives as drugs and pesticides.


Introduction
Terpenoids containing a variety of biological activities, are the promising lead structures for the development of drugs and pesticides [1][2][3][4][5][6]. Rosin is an important natural renewable resource in pine trees [7,8]. Dehydroabietic acid (1, Figure 1), a tricyclic diterpenoid, is a natural resin acid isolated from rosin and shows a wide range of biological activities [9]. The content of dehydroabietic acid in rosin was low, and it was not suitable for direct extraction of dehydroabietic acid. Storage-stable dehydroabietic acid can be purified by disproportionating abietic acid or rosin. In the industry, disproportionated rosin was obtained by disproportionation reaction of rosin as raw material, and then dehydroabietic acid can be obtained by organic amine salt and solvent recrystallization methods. Therefore, the research focus of purification was mainly on the preparation of disproportionated rosin, and the most effective catalyst for the disproportionation process of rosin was the expensive Pd/C catalyst, so it was very important to seek an efficient and economical purification condition [10]. In addition, dehydroabietic acid can also be isolated from two cyanobacteria strains [11].
The molecular skeleton of dehydroabietic acid contains one carboxyl group, one aromatic ring, and two alicyclic rings, with a total of twenty carbons. Dehydroabietic acid and its derivatives showed a variety of biological activities such as antiviral [12], antitumor [13], wound-healing [14], antiulcer [15], gastroprotective [16], anxiolytic [17], herbicidal [18] and antibacterial properties [19]. Furthermore, Xie et al. found that dehydroabietic acid had insecticidal activity against Peridroma saucia (Lepidoptera: Noctuidae) [20]. It demonstrated that larch sawfly exposure to dehydroabietic acid resulted in reduced feeding and slowed growth [21]. Dehydroabietic acid acted as an antagonist of insect juvenile hormone, interfering with the endocrine regulation of insects [22], and its derivatives also had an attracting effect on Spodoptera litura [23]. Recently, Xin et al. reported that novel multifunctional nanomedicines assembled from chitosan oligosaccharide-melanin complexes and dehydroabietic acid hexamers can achieve efficient and precise treatment of tumors [24]. Huang et al. found that biomass-based carbon dots prepared from dehydroabietic acid by hydrothermal reaction not only can sensitively and selectively detect heavy metal ions, but also can be used for cell imaging with low cytotoxicity [25]. However, while its biological activities were diverse, it had certain toxic and side effects. It reported that resin acids may be toxic to fish [26,27]. Based on the above studies, with the characteristics of good stability (due to the aromatic ring) and wide biological activities, dehydroabietic acid was a candidate of interest in the fields of medicine and agriculture. Moreover, to solve the disadvantages of its toxic and side effects, it was necessary to develop new dehydroabietic acid derivatives with high bioactivity and low toxicity. Therefore, this review summarized the structural modifications and biological activities of dehydroabietic acid and its derivatives from 2015 to 2021.

Antitumor Activity
Dehydroabietic acid derivatives exhibited anti-cancer activity through various mechanisms including inhibiting tumor cell migration and inducing tumor apoptosis [7]. Lee et al. found that dehydroabietic acid induced apoptosis of human lung cells by inducing the division of caspase-3 and PARP in these cells and interfering with mitochondria [28]. Moreover, Luo et al. suggested that a derivative of dehydroabietic acid (2, Figure 2), maybe a therapeutic drug for gastric cancer because it induced damage to cell membranes and organelles, and ultimately led to apoptosis of gastric cancer cells [29]. Through flow cytometry and cell cycle analysis, it was found that compound 3 ( Figure 2) can induce HepG2 cell apoptosis and block the HepG2 cell line in the G1 phase to exert anti-cancer effects [30]. Dehydroabietic oxime was a possible method for the treatment of pancreatic cancer and its related inflammation. It can up-regulate the level of p27, and it can also down-regulate the expression of cyclin D1, thereby preventing the growth of pancreatic cancer cells in the G1 phase [31]. Recently, some dehydroabietic acid derivatives were potential drugs targeting the kinase domain of EGFR, which showed anticancer activity against HepG2 cancer cell lines [32].

Anti-Inflammatory Activity
Kim et al. evaluated the anti-inflammatory effects of dehydroabietic acid and found that not only the production of nitric oxide (NO) in the macrophage cell line was reduced under the action of dehydroabietic acid, but also the expression of inflammatory genes was descended. In addition, through the treatment of dehydroabietic acid, both the activity of kinases in the NF-κB cascade and TAK1 (transforming growth factor β-activated kinase 1) in the AP-1 cascade was inhibited [33]. A study conducted by Kang et al. proved that the treatment of dehydroabietic acid was an effective way to ameliorate inflammatory changes related to obesity-related diabetes. It demonstrated that dehydroabietic acid was an activator of PPARα and PPARγ and can inhibit the production of MCP-1, TNF-α, and NO (pro-inflammatory mediators) [34].

Antibacterial and Antifungal Activities
Experiments by Tretyakova et al. proved that pyrrolidine-containing dehydroabietic acid acetylene derivatives (4, Figure 3) showed growth inhibitory effects on the fungi Candida albicans and Cryptococcus neoformans, and had low hemolytic capacity [35]. In addition, Chen et al. reported that the derivative of dehydroabietic acid (5, Figure 3) exhibited excellent antibacterial activity against Gram-positive bacteria such as Bacillus subtilis and Staphylococcus aureus with the MIC (minimum inhibitory concentration) value of 4 and 2 µg/mL, respectively [36]. Dehydroabietic acid-containing serine derivatives had excellent antibacterial activity against Gram-positive bacteria. For example, the MIC 90 values of compound 6 ( Figure 3) against methicillin resistant S. aureus, Staphylococcus epidermidis, and Streptococcus mitis were 8 µg/mL [19]. Furthermore, through the broth micro-dilution method, it was found that dehydroabietic acid showed antibacterial activity against four kinds of Streptococcus mutans (including S. mutans ATCC 12175, S. mutans NRPC 801, S. mutans NRPC 804, and S. mutans DMST 18777) [37]. Hassan et al. used dehydroabietic acid derivatives to prepare antibacterial nanocellulose membranes to solve the inevitable toxicity of silver and cationic antimicrobial agents. By simulating the physiological environment of chronic wounds, they found that it prevented the colonization of bacteria on the surface [38]. It was revealed that the dehydroabietic acid analog (7, Figure 3) exhibited antimicrobial activity against methicillin resistant S. aureus (MIC: 32 µg/mL) [39]. It is urgent to develop new antibacterial agents against methicillin-resistant and methicillin-sensitive S. aureus infections (MRSA and MSSA). Compound 8 ( Figure 3) had effective inhibitory activity against the above-mentioned bacteria, and its MIC values were from 3.9 to 15.6 µg/mL. Moreover, it had no cytotoxicity and hemolytic activity in mammalian cells [40]. In addition, Liu et al. elucidated that some 12-oxime and O-oxime ether derivatives of dehydroabietic acid had strong antistaphylococcal activity. Compound 9 showed high inhibitory activity against S. aureus Newman with MIC value of 0.39-0.78 µg/mL, while compounds 10−12 had MIC values of 1.25-3.13 µg/mL for multidrug-resistant S. aureus. [41]. Additionally, some MIC values of dehydroabietic acid derivatives were listed in Table 1.

Insecticidal Activity
Dehydroabietic acid displayed antifeedant activity against Indian meal moth Plodia interpunctella, but had no obvious effect on the growth of larvae [22]. Additionally, dehydroabietic acid had certain insecticidal activity against Aedes aegypti larvae, with a lethality rate of 65% at 10 ppm [42]. Gao et al. found that dehydroabietic acid amide derivatives containing the thiadiazole fragment (13a−13c, Figure 4) exhibited remarkable insecticidal activities against diamondback moth (Plutella xylostella) with the LC 50 of 0.222-0.224 µg/mL [43]. Dehydroabietic acid had good antifeedant activity and a certain toxic effect on Mythimna separata larvae [44]. Moreover, the mountain pine beetle was often affected by the chemical defense of host oleoresin secretions which contained dehydroabietic acid [45].

Antiprotozoal Activity
Dehydroabietic acid not only inhibited the proliferation of the promastigote form, but also induced the production and output of ROS (reactive oxygen species) and downregulated Nrf2 (nuclear factor erythroid 2-related factor 2) to exert its anti-Leishmania activity, mainly due to its antioxidant properties [46]. Pertino et al. reported that dehydroabietic acid had certain antiprotozoal activity against Trypanosoma cruzi, Leishmaniabraziliensis, and Leishmania infantum [47]. Moreover, the dehydroabietic acid derivative containing amino acid structure was a new and effective antiprotozoal drug with good selectivity to Leishmania donovani and T. cruzi [48].

Other Activities
The treatment of dehydroabietic acid alleviated insulin resistance and liver steatosis caused by a high-fat diet in mice as a dual agonist of PPAR-α/γ [49]. Moreover, the antiobesity effect of dehydroabietic acid may be exerted by improving the levels of plasma glucose and insulin via PPARα/γ-dependent pathways [34,50,51]. Dehydroabietic acid reduced hypertrophy by activating the Keap1/Nrf2-ARE signaling pathway, increasing the expression of the ferroptosis suppressor protein 1 (FSP1) gene, and inhibiting the accumulation of ROS to ameliorate non-alcoholic fatty liver disease [52]. Dehydroabietic acid inhibited angiotensin converting enzyme in human umbilical vein endothelial cells and induced p-Akt, which could reduce the systolic blood pressure of spontaneously hypertensive rats, thereby exerting an anti-hypertensive effect [53]. Dehydroabietic acid as an anti-aging agent could directly bind to SIRT1 protein and activated SIRT1 [54]. Park et al. suggested that dehydroabietic acid protected and regenerated the collagen fibers in the skin irradiated by ultraviolet B [55]. In addition, through in vivo and in vitro experiments, it was found that the assembled dehydroabietic acid derivative (14, Figure 5)/ZnAlTi-LDH (layered double hydroxide) composite material can promote wound healing, kill Gramnegative and positive bacteria infecting the wound, and block ultraviolet rays to protect the skin [56]. Since dehydroabietic acid can promote osteogenic differentiation, it may be a new idea for the treatment of osteoporosis in the elderly [57]. Through in vitro experimental studies, Nachar et al. found that dehydroabietic acid may be an effective drug for the treatment of type 2 diabetes. It not only reduced the activity of G6Pase to descend glucose production but also stimulated GS activity to ascend glucose storage [58]. Additionally, a dehydroabietic acid polymer (15, Figure 5) can be used as a toner for xerography [59]. BK channels, K + channels activated by large-conductance calcium, had vital physiological functions such as the release of neurotransmitters. A thiophene derivative of dehydroabietic acid (16, Figure 5) showed BK channel opening activity [60,61]. A dehydroabietic acid derivative (17, Figure 5) can be used as fluorescent probes and can sensitively detect Fe 3+ and Hg 2+ ions in cells [62]. Moreover, dehydroabietic acid derivatives (18 and 19, Figure 5) as natural resources provided a new method for the synthesis of fluorescent materials [63]. Subsequently, compounds containing thiourea and bisphosphonates (28a−28k) were synthesized as effective antitumor agents (Scheme 2). Compound 28e showed the best anticancer activity against the SK-OV-3 cell line with an IC 50 value of 1.79 ± 0.43 µM, which arrested the cell cycle in the G1 phase and induced apoptosis [65]. As shown in Scheme 2, a novel class of dehydroabietic acid acyl-thiourea derivatives (29(a-o)−30(a-o)) were obtained and their antitumor activity was evaluated against HeLa, SK-OV-3, HL-7702, and MGC-803 cell lines. Most of these compounds demonstrated cytotoxicity, especially compound 30n (IC 50 : 6.58 ± 1.11 µM against HeLa) exhibited better inhibitory effect than 5-Fu (a commercial antitumor drug, IC 50 : 36.58 ± 1.55 µM). Moreover, compound 30n could block HeLa cells in the S phase and induce HeLa cell apoptosis via the mitochondrial pathway [66]. In 2020, Li et al. designed and synthesized compounds 31 and 32 (Scheme 2). Compared with cisplatin and oxaliplatin, compound 31 had better cytotoxic activity on A431 cells and a stronger ability to bind with DNA [67]. Moreover, compounds 33a−33m (Scheme 2) were synthesized as antitumor and antimicrobial agents. Compound 33e, a MIC value of 1.9 µg/mL, demonstrated the most potent inhibitory activity against B. subtilis. Meanwhile, compound 33m showed anticancer activity comparable to the positive control etoposide [68].    Additionally, dehydroabietic acid containing 1,2,3-triazole derivatives (41a−41p, Scheme 5) were prepared. The most potential compounds 41c (5.90 ± 0.41 µM) and 41k (6.25 ± 0.37 µM) exhibited better antiproliferation activity against HepG2 cells than that of cisplatin [71]. Compound 43b showed good inhibitory activity on the tested cells (IC 50 : 7.00-11.93 µM), but it had a little toxic effect on normal cells. The mechanism of action of compound 43b was to block MCF-7 cells in the S phase and induced apoptosis [72]. Scheme 6. Synthesis of compounds 43a−43o.
As described in Scheme 7, Li et al. synthesized a class of dehydroabietic acid-nitrate conjugates (47a−47r) as antitumor agents. Compound 47n showed the most promising activity against the BEL-7402 cell line (IC 50 : 11.23 ± 0.21 µM). Compound 47j exhibited the most potent cytotoxicity against the CNE-2 cell line (IC 50 : 8.36 ± 0.14 µM) and evaluation of NO release indicated that the cytotoxic activity improved with the increase of the amount of NO produced in the CNE-2 cell line [73].
In 2018, Zhang et al. synthesized a series of N-sulfonaminoethyloxime derivatives of dehydroabietic acid from compound 55 (Scheme 10). The MIC value of analog 59w against S. aureus Newman was 0.39-0.78 µg/mL, exhibiting the highest activity. In addition, the remaining analogs also showed better antibacterial activities against five multi-drug resistant S. aureus, with MIC values ranging from 0.78 to 1.56 µg/mL [79].
A series of hydrazone derivatives of dehydroabietic acid (61a−61g, Scheme 11) were synthesized. All compounds showed antibacterial activities against Escherichia coli, S. aureus, and B. subtilis. Especially compound 61d was the potent antimicrobial agent against B. subtilis and S. aureus [80].

Scheme 11. Synthesis of compounds 61a−61g.
Chen et al. synthesized a series of dehydroabietic acid derivatives (63a−63t, Scheme 12) to find novel and effective antitumor agents, and tested in vitro cytotoxic activity against HepG2, SCC9, and 293T by CCK-8 method. Compounds 63r and 63s had a certain cytotoxic activity to cancer cells, but were weak to normal cells. Two compounds could inhibit the PI3K/AKT/mTOR signaling pathway to exert anti-cancer effects. The results of molecular docking studies indicated that the compounds may inhibit the pathway through ATP competition [81].

Structural Modification at C Ring of Dehydroabietic Acid
In order to find effective new antimicrobial agents, a class of new derivatives of dehydroabietic acid (69a−69o, Scheme 13) was obtained. Compound 69o showed excellent antibacterial activity against both Gram-negative and positive bacteria (MIC: 1.6-3.1 µg/mL) but had no obvious toxicity to mammalian cells. In addition, compound 69o (containing 1,2,3-triazole moiety at the C-14 position) had good drug-like properties [83]. Additionally, compound 69p (IC 50 values from 0.7 to 1.2 µM) not only induced apoptosis of MDA-MB-231 cells but also had weak toxicity to normal cells. And its anti-proliferative activity was better than 5-Fu (average IC 50 : 16.1 µM) [84]. Several derivatives of dehydroabietic acid containing 12-thiazole moiety (71a−71e) were designed and synthesized as shown in Scheme 14. In order to understand the importance of hABHD16A inhibition in vivo, the feasibility of these derivatives as a starting point for the design of selective ABHD16A (a new target for inflammation-mediated pain) inhibitors was investigated. Compound 71d had an IC 50 value of 3.4 ± 0.2 µM with good selectivity [85]. Gu et al. studied the cytotoxicity of a series of newly synthesized dehydroabietic acid derivatives (74(a-k)−75(a-k), Scheme 15) on liver cancer cells and found that most of the compounds had obvious cytotoxic activity on SMMC-7721 and HepG2 liver cancer cells with low toxicity on normal human liver cells. Among them, compounds 74b and 74e showed the best cytotoxicity against SMMC-7721 and HepG2 cells (IC 50 values: 0.36 ± 0.13 and 0.12 ± 0.03 µM, respectively). In addition, compound 74b not only caused SMMC-7721 cells' cell cycle arrest in the G2/M phase, but also induced apoptosis [86].
Miao et al. synthesized a series of novel 2-aryl-benzimidazole derivatives of dehydroabietic acid (80(a-k)−81(a-k), Scheme 16). Compound 80j exhibited the strongest cytotoxic activity with the IC 50 value of 0.08-0.42 µM. In addition, further in-depth exploration elucidated that compound 80j significantly inhibited the migration ability of SMMC-7721 cells, and induced cell cycle arrest and apoptosis of the cells in the G2/M phase. Through tubulin polymerization and immunofluorescence assays, it was found that compound 80j not only inhibited tubulin polymerization but also destroyed the intracellular microtubule network [89].

Biotransformation of Dehydroabietic Acid
Possible pathways for the biotransformation of dehydroabietic acid by Mucor circinelloides IT25, Mortierella isabellina HR32, Moraxella sp. HR6, Trametes versicolor, Phlebiopsis gigantea, Flavobacterium resinovorum, Fusarium oxyosporum/F. moniliforme and Fomes annosum were shown in Scheme 18. These microorganisms were some of the previously reported microorganisms with hydroxylated metabolites. Two molds (M. circinelloides IT 25 and M. isabellina HR32) regioselectively and stereoselectively hydroxylated dehydroabietic acid to 2α-hydroxydehydroabietic acid (85). Dehydroabietic acid was oxidized at the C-3 and C-7 positions, decarboxylated at the C-4 position by the action of bacteria Moraxella sp. HR6, and finally converted to 3,7-dioxodehydroabietin (86) [91]. The first step in the degradation of dehydroabietic acid was caused by two fungi, T. versicolor and P. gigantea, and a stereoselective hydroxylation was at the C-1 position, followed by further hydroxylation at the C-7 or C-16 position to form dihydroxylated compounds 89 and 91. Compound 87 or 91 was further hydroxylated at the C-1 or C-7 position, resulting in trihydroxylated compound 92. The hydroxyl group at the C-7 position of compounds 91 and 92 can be further oxidized to a carbonyl functional group to afford compounds 93 and 94 [92,93].

Total Synthesis of Dehydroabietic Acid
Dehydroabietic acid has attracted the attention of researchers because of its diverse biological activities. However, separation and purification of dehydroabietic acid usually required expensive catalysts, and the organic amine salt method was more toxic and polluted the environment [10]. Therefore, it was particularly important to find other methods for synthesis of dehydroabietic acid. Some total synthesis processes of dehydroabietic acid were as follows.
In 1956, the first synthesis of dehydroabietic acid was begun with 2-isopropylnaphthalene (95) as the starting material (Scheme 19). The most important step was to define the C-4 center, that is, to alkylate phenanthrone (96) with ethyl bromoacetate. The enone (96) was then alkylated to obtain the keto ester (97), which reacted with HSCH 2 CH 2 SH to form the thioketal (98). The thioketal (98) was converted into its methyl ester (99) by ester hydrolysis and diazotization reactions. After Raney nickel desulfurization, ester hydrolysis, and Pd/C hydrogenation, intermediate 100 was obtained, which reacted with CH 2 N 2 to produce methyl ester compound 101. The second method started with 2-methyl-2-(p-isopropylphenyl)cyclohexanone (104) was described in Scheme 20. The key steps were stereoselective alkylation of 105 with KOt-Bu and MeI, and reduction with Li in liquid ammonia to give decalone 107, which reacted with HCO 2 Et, O 3 , and polyphosphoric acid (PPA) to give tricyclic keto acid 109. After the hydrogenolysis reaction, the amine oxide pyrolyzed to afford the vinylic compound 110, which reacted with OsO 4 and HIO 4 to give the corresponding aldehyde (111). Finally, dehydroabietic acid (1) was obtained by reaction of 111 with NH 2 OH and KOH [94].
The third method was a short enantioselective synthesis starting from the geranyl acetate derivative (112) as shown in Scheme 21. Compound 112 was converted to alkene 113 by the Wittig reaction, which was further catalyzed by Li 2 CuCl 4 to give compound 114. The enantioselective cyclization of 114 and 115/SbCl 5 complex afforded compound 116, which was a key step in the reaction. Finally, compound 116 reacted with KMnO 4 and NaIO 4 to obtain dehydroabietic acid (1) [94].

Scheme 21.
Total synthesis of dehydroabietic acid from the geranyl acetate derivative.

Structure-Activity Relationships of Dehydroabietic Acid and Its Derivatives
The SARs analysis of dehydroabietic acid and its derivatives about the antibacterial and anticancer activities ( Figure 6) were as follows: (1) For antibacterial activity: (a) Introduction of trifluoromethyl phenyl, pyrrolyl, or substituted thiophenyl groups (57h−57j and 57p−57s) into the oxime derivatives at the C-7 position of dehydroabietic acid was beneficial to the antibacterial activity [78]. C-7 Acylhydrazone derivatives of dehydroabietic acid (especially p-fluorobenzoyl hydrazone) can increase the antibacterial activity [80]. (b) Introduction of 1,3,4-thiadiazole-thiazolidinone into the C-18 carboxyl group of dehydroabietic acid can effectively improve the antibacterial activity [76]. (c) Introduction of 1,2,3-triazole ring at the C-14 position of dehydroabietic acid can improve the antibacterial activity [83].
(2) For anticancer activity: (a) Introduction of the C=N-OH group at the C-7 position can improve anti-tumor activity [64]. (b) Introduction of thiourea and bisphosphonate groups into the C-18 position of dehydroabietic acid was beneficial to the anticancer activity [65]. (c) 1,2,3-Triazole ring at the C-18 position played a key role for dehydroabietic acid derivatives showing the anti-cancer effect [71,84]. (d) C-12 sulfonylurea derivatives of dehydroabietic acid bearing electron-withdrawing groups at the ortho or para position on the N-substituted aromatic ring, can improve the antiproliferative activity [90].
Researchers expected to synthesize high bioactive compounds with low toxicity. Herein, we summarized some IC 50 values of the most active derivatives against human normal cell lines ( Table 4). Most of these derivatives exhibited more potent activity than dehydroabietic acid against cancer cell lines, and they showed low cytotoxicity against human normal cells. The IC 50 value of compound 36w against HeLa cells (2.21 ± 0.04 µM) was about 17-folds higher than that of the precursor (37.40 ± 0.64 µM), while the cytotoxicity of compound 36w against normal HL-7702 human liver cell was lower (66.08 ± 1.84 µM) [69]. Compound 39j exhibited good cytotoxicity against the tested cancer cell lines (IC 50 values:.82-17.76 µM) compared to the lead compound, with no increase in cytotoxicity against normal cells (IC 50 > 100 µM) [70]. In addition, the anticancer activity of compounds 41c, 41k, 47j, and 47n was significantly improved, but their toxicity against human normal cells was not increased compared to that of dehydroabietic acid; that is, they exhibited excellent selectivity between normal and cancer cells [71,73]. The cytotoxicity of compound 77b against normal human liver cell LO2 (IC 50 : 11.09 ± 0.57 µM) was much lower than that of compound 77b against MCF-7, SMMC-7721, and HeLa cancer cells (IC 50 values: 0.72-1.78 µM). However, compared with that of dehydroabietic acid (IC 50 > 50 µM), it was more toxic to normal cells [87]. Compound 79h was also more cytotoxic against normal human LO2 cell (IC 50 : 42.83 ± 3.18 µM) than dehydroabietic acid (IC 50 > 50 µM) [88]. Based on the above results, when compared with the precursor, the bioactivity of some compounds was significantly improved after structural modifications, but their toxicity against normal human cells was also increased. Fortunately, compounds 39j, 41c, 41k, 47j, and 47n not only showed promising biological activity, but also had good selectivity between normal and malignant cells; so they can be further studied and developed as potential anticancer drugs.

Conclusions
Dehydroabietic acid, a tricyclic diterpenoid resin acid, showed a wide range of biological activities. The structural characteristics suggested that dehydroabietic acid had multiple structural modification sites, and using it as a lead compound can improve its application value. This review gave an overview of the biological activities, structural modifications, biotransformation, total synthesis, and structure-activity relationships of dehydroabietic acid and its derivatives. Although several structural modifications of dehydroabietic acid and its derivatives were summarized, their properties were mainly focused on anticancer and antibacterial activities. Obviously, their agricultural and other activities needed to be expanded in the future. Therefore, we hope that this review can provide references for further research and development of dehydroabietic acid and its derivatives, as well as their applications as drugs and pesticides in the medical and agricultural fields.

Conflicts of Interest:
The authors declare no conflict of interest.