Interacting Environmental Stress Factors Affects Targeted Metabolomic Profiles in Stored Natural Wheat and That Inoculated with F. graminearum

Changes in environmental stress impact on secondary metabolite (SM) production profiles. Few studies have examined targeted SM production patterns in relation to interacting environmental conditions in stored cereals. The objectives were to examine the effect of water activity (aw; 0.95–0.90) x temperature (10–25 °C) on SM production on naturally contaminated stored wheat and that inoculated with Fusarium graminearum. Samples were analysed using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) on (a) total number of known SMs, (b) their concentrations and (c) changes under environmental stress. 24 Fusarium metabolites were quantified. Interestingly, statistical differences (ChisSq., p < 0.001) were observed in the number of SMs produced under different sets of interacting environmental conditions. The dominant metabolites in natural stored grain were deoxynivalenol (DON) and nivalenol (NIV) followed by a range of enniatins (A, A1, B, B1), apicidin and DON-3-glucoside at 10 °C. Increasing temperature promoted the biosynthesis of other SMs such as aurofusarin, moniliformin, zearalenone (ZEN) and their derivatives. Natural wheat + F. graminearum inoculation resulted in a significant increase in the number of metabolites produced (ChisSq., p < 0.001). For ZEN and its derivatives, more was produced under cooler storage conditions. Fusarin C was enhanced in contrast to that for the enniatin group. The relative ratios of certain groups of targeted SM changed with environmental stress. Both temperature and aw affected the amounts of metabolites present, especially of DON and ZEN. This study suggests that the dominant SMs produced in stored temperate cereals are the mycotoxins for which legislation exists. However, there are changes in the ratios of key metabolites which could influence the relative contamination with individual compounds. Thus, in the future, under more extreme environmental stresses, different dominant SMs may be formed which could make present legislation out of step with the future contamination which might occur.


Introduction
In cereal commodities, it is important to ensure that drying and storage is effective to ensure that spoilage moulds and mycotoxin contamination can be prevented or minimised [1]. It is important in temperate cereals that the moisture content of 14.5-15% (wet weight basis = 0.70 water activity, a w ) is not exceeded to ensure stable medium-term storage without spoilage and any post-harvest mycotoxin contamination. In the case of wheat/barley there are clear guidelines with regard to the EU legislative limits for contamination with type B trichothecenes (deoxynivalenol, DON) and also for Zearalenone (ZEN). However, less information is known about the range of secondary metabolites (SMs) which may be present due to fungal colonisation and whether there are differences in the relative production of different related compounds. Certainly, interacting environmental stresses of a w x temperature has been shown to change the relative amounts of related DON metabolites (DON, 3-and 15-acetyl DON and Nivalenol (NIV) [2].
The development of both molecular and analytical techniques has resulted in significant interest in the microbiomes of different food commodities and the production of different SMs which may impact on food quality/safety. For example, recently Zhang et al. [3] examined both the fungal and bacterial microbiomes and the SM profile and changes in Pu-erh tea during fermentation. They identified the changes in bacterial and fungal communities and extrolites present and found 25 toxic metabolites, mainly of fungal origin present. Patulin and asperglaucide were the dominant SMs. For cereals, some studies have examined SM profiles and compounds in single maize kernels and stored grain [4,5]. They isolated a battery of SMs from maize kernels which suggested that the co-occurrence of different SMs may be important and have a significant impact on rural consumers in Nigeria and South Africa. A recent study on isolation of different SMs from stored wheat and correlation with molecular approaches suggested that aflatoxin B 1 , fumonisins and DON were the most common toxins present. They found a strong correlation between the presence of mycotoxin biosynthesis genes analysed by multiplex PCR and mycotoxin detection by LC/MS/MS. However, in this study, most of the wheat samples examined were stored under safe moisture conditions of <14.5% m.c. [6]. Thus, there is a lack of knowledge on what range of SMs may co-occur in wheat stored under non-conducive and conducive environmental conditions for colonisation by spoilage and mycotoxigenic fungi.
A previous study of durum wheat inoculated with Fusarium poae examined the volatile organic compounds (VOCs) in inoculated and un-inoculated grain [7]. A total of 29 volatile markers were selected among the detected compounds and multivariate analysis was applied to establish the relationship between potential volatile biomarkers and fungal contamination. A range of VOCs, including alcohols, ketones, esters, furans and aromatics, were identified, both in contaminated and in healthy kernels. However, the overall volatile profile of infected samples and controls differed, indicating that the whole volatile profile, rather than individual volatile compounds, could be used to identify F. poae contamination of durum wheat grains [7]. However, again, this study did not examine different a w x temperature storage conditions. Previously, it has been suggested that SM biosynthesis may be related to VOCs with toxigenic strains producing different biomarkers when compared to non-toxic strains in Fusarium section Liseola strains of F. verticillioides and F. proliferatum and also in relation to Aspergillus section Nigri species/strains [8,9].
The objectives of this study were to examine the effect of storage of natural wheat grain and that inoculated with F. graminearum stored at 0.90, 0.93, 0.95 a w and 10-25 • C on (a) the range of mycotoxins and related SMs which may be produced, (b) their ranges and ratios of production. Storage conditions represented those which were non-conducive and conducive to colonisation and SM contamination.

Natural Fungal Contamination
Similar initial fungal population levels (CFUs/g dry weight) were isolated on both DG18 and MEA with Log 3.3 ± 1.3 and Log 2.4 ± 0.6 CFUs/g respectively. Fusarium (36-73%) was the main genus isolated, with other species isolated from the Alternaria, Acremoniun, Cladosporium, Epicocum and Penicillium genera ( Figure 1). Penicillium species were only isolated from grain plated on DG18 medium. In contrast, Epicocum species were only isolated from wheat grain on MEA and Acremonium species from surface sterilized kernels. isolated, with other species isolated from the Alternaria, Acremoniun, Cladosporium, Epicocum and Penicillium genera ( Figure 1). Penicillium species were only isolated from grain plated on DG18 medium. In contrast, Epicocum species were only isolated from wheat grain on MEA and Acremonium species from surface sterilized kernels.

Patterns of Total Targeted Metabolite Production in Relation to Interacting Environmental Factors in Naturally Stored Wheat and That Inoculated with F. graminearum
The naturally contaminated dry wheat grain (14.5% moisture content) used in this study contained 15 different SMs. These included DON, aurofusarin, enniatins, beauvericin and apicidin. Figures 2 and 3 show the total numbers of SMs produced (out of 121) under the different storage conditions in naturally stored wheat and that with F. graminearum. They show the relative number of compounds produced in different categories based on the concentrations (5, 50, 500 ng/g) produced.
In both treatments as temperature was increased the total number of SMs and their concentrations increased (ChiSquare, p < 0.05). However, in natural wheat non-parametric comparisons for each pair using the Wilcoxon method showed that the main differences in SMs produced at >5 and >50 ng/g was between 15-25 °C and that at 10 °C. aw did not affect the total number of SMs produced. However, significant differences were found between 0.90-0.95 aw (ChiSquare, p < 0.05) when examining the SM concentrations produced at >50 and >500 ng/g in both naturally stored wheat and that inoculated with F. graminearum.
Additionally, a linear discriminant analysis (LDA) was performed including all the variables (p < 0.05) (see Supplementary Figure S1). A model including 15 variables showed significant discrimination (Wilks' Lambda < 0.0001) between natural and wheat inoculated with F. graminareaum (Supplementary Table S1-S4). Indeed, in 92 samples only 5.4% were misclassified and all of them belonged to the inoculated group. Interestingly, for the Fusarium metabolites, neither ZEN nor NIV were significant. Thus, these were not included in the model. In contrast, another SM, chrysogine, could be an indicator of contamination.

Patterns of Total Targeted Metabolite Production in Relation to Interacting Environmental Factors in Naturally Stored Wheat and That Inoculated with F. graminearum
The naturally contaminated dry wheat grain (14.5% moisture content) used in this study contained 15 different SMs. These included DON, aurofusarin, enniatins, beauvericin and apicidin. Figures 2 and 3 show the total numbers of SMs produced (out of 121) under the different storage conditions in naturally stored wheat and that with F. graminearum. They show the relative number of compounds produced in different categories based on the concentrations (5, 50, 500 ng/g) produced.
In both treatments as temperature was increased the total number of SMs and their concentrations increased (ChiSquare, p < 0.05). However, in natural wheat non-parametric comparisons for each pair using the Wilcoxon method showed that the main differences in SMs produced at >5 and >50 ng/g was between 15-25 • C and that at 10 • C. a w did not affect the total number of SMs produced. However, significant differences were found between 0.90-0.95 a w (ChiSquare, p < 0.05) when examining the SM concentrations produced at >50 and >500 ng/g in both naturally stored wheat and that inoculated with F. graminearum.
Additionally, a linear discriminant analysis (LDA) was performed including all the variables (p < 0.05) (see Supplementary Figure S1). A model including 15 variables showed significant discrimination (Wilks' Lambda < 0.0001) between natural and wheat inoculated with F. graminareaum (Supplementary Table S1-S4). Indeed, in 92 samples only 5.4% were misclassified and all of them belonged to the inoculated group. Interestingly, for the Fusarium metabolites, neither ZEN nor NIV were significant. Thus, these were not included in the model. In contrast, another SM, chrysogine, could be an indicator of contamination.  Overall there were more SMs produced in stored wheat grain with additional colonization by F. graminearum (ChiSquare, p < 0.05). It was noticeable that at 15-25 °C and 0.90-0.95 aw the numbers of SMs produced did not change markedly, whether considering the total number or those which were present at >50 or at >500 ng/g concentrations. These SMs were divided into those originating from colonization by Fusarium, Penicillium and Alternaria species in both natural and F. graminearum colonized wheat grain. For Penicillium metabolites, between 12-20 were produced, at 15-25 °C, at all  Overall there were more SMs produced in stored wheat grain with additional colonization by F. graminearum (ChiSquare, p < 0.05). It was noticeable that at 15-25 °C and 0.90-0.95 aw the numbers of SMs produced did not change markedly, whether considering the total number or those which were present at >50 or at >500 ng/g concentrations. These SMs were divided into those originating from colonization by Fusarium, Penicillium and Alternaria species in both natural and F. graminearum colonized wheat grain. For Penicillium metabolites, between 12-20 were produced, at 15-25 °C, at all Overall there were more SMs produced in stored wheat grain with additional colonization by F. graminearum (ChiSquare, p < 0.05). It was noticeable that at 15-25 • C and 0.90-0.95 a w the numbers of SMs produced did not change markedly, whether considering the total number or those which were present at >50 or at >500 ng/g concentrations. These SMs were divided into those originating from colonization by Fusarium, Penicillium and Alternaria species in both natural and F. graminearum colonized wheat grain. For Penicillium metabolites, between 12-20 were produced, at 15-25 • C, at all the a w levels examined in both naturally stored wheat and that inoculated with F. graminearum (Supplementary Figure S2a,b). For Alternaria metabolites, out of a possible 6 metabolites, at least 2-4 were often produced, regardless of environmental stress imposed (Supplementary Table S5). It is worth noting that the presence of a higher initial inoculum of F. graminearum (inoculation treatment) in wheat promoted the number of the SMs produced. This is indicated by the concentrations of SMs produced by Penicillium and Alternaria species. For example, altersolanol (AS) was not detected in natural wheat but was present in the wheat + F. graminearum treatments, especially at 0.95 a w and 20-25 • C.

Trichothecenes, Zearalenone and Related Fusarium Metabolites in Naturally Stored Wheat and That Inoculated with F. graminearum
Twenty-four different SMs produced by Fusarium were quantified in natural wheat grain and that inoculated with F. graminearum after 15 days storage. More Fusarium metabolites were produced in the stored wheat grain + F. graminearum treatment (ChiSquare, p < 0.05). Figure 4 shows the relative amounts of different Fusarium-related SMs isolated. Overall, between 14-16 compounds were present in the both naturally stored wheat and that inoculated with F. graminearum. This shows the key changes in the relative proportions of enniatins, type B trichothecenes and ZEN-related compounds found. the aw levels examined in both naturally stored wheat and that inoculated with F. graminearum (Supplementary Figure S2a,b). For Alternaria metabolites, out of a possible 6 metabolites, at least 2-4 were often produced, regardless of environmental stress imposed (Supplementary Table S5). It is worth noting that the presence of a higher initial inoculum of F. graminearum (inoculation treatment) in wheat promoted the number of the SMs produced. This is indicated by the concentrations of SMs produced by Penicillium and Alternaria species. For example, altersolanol (AS) was not detected in natural wheat but was present in the wheat + F. graminearum treatments, especially at 0.95 aw and 20-25 °C.

Trichothecenes, Zearalenone and Related Fusarium Metabolites in Naturally Stored Wheat and That Inoculated with F. graminearum
Twenty-four different SMs produced by Fusarium were quantified in natural wheat grain and that inoculated with F. graminearum after 15 days storage. More Fusarium metabolites were produced in the stored wheat grain + F. graminearum treatment (ChiSquare, p < 0.05). Figure 4 shows the relative amounts of different Fusarium-related SMs isolated. Overall, between 14-16 compounds were present in the both naturally stored wheat and that inoculated with F. graminearum. This shows the key changes in the relative proportions of enniatins, type B trichothecenes and ZEN-related compounds found.  (Table 1), (b) type B trichothecenes (Table 2), (c) enniatins (Table 3) and (d) other Fusarium metabolites ( Table 4). The Kruskal-Wallis test by ranks was used for the analysis of the effect of storage conditions and inoculation with F. graminearum on production of different Fusarium-related SMs. ZEN levels were significantly higher in stored wheat + F. graminearum (p < 0.05) inoculum treatments. For ZEN and associated compounds isolated from both types of wheat treatments, temperature significantly (p < 0.05) affected ZEN contamination, while aw was only significant in stored wheat inoculated with F. graminearum. A similar trend was observed in the derivatives, alphazearalenone, beta-zearalenone and zearalenone-sulphate found in these stored wheat treatments (see Table 1). Temperature significantly (p < 0.05) affected the production of ZEN, while aw was only significant in naturally contaminated stored wheat.  (Table 1), (b) type B trichothecenes (Table 2), (c) enniatins (Table 3) and (d) other Fusarium metabolites ( Table 4). The Kruskal-Wallis test by ranks was used for the analysis of the effect of storage conditions and inoculation with F. graminearum on production of different Fusarium-related SMs. ZEN levels were significantly higher in stored wheat + F. graminearum (p < 0.05) inoculum treatments. For ZEN and associated compounds isolated from both types of wheat treatments, temperature significantly (p < 0.05) affected ZEN contamination, while a w was only significant in stored wheat inoculated with F. graminearum. A similar trend was observed in the derivatives, alpha-zearalenone, beta-zearalenone and zearalenone-sulphate found in these stored wheat treatments (see Table 1).
Temperature significantly (p < 0.05) affected the production of ZEN, while a w was only significant in naturally contaminated stored wheat.
The contamination profile of type B trichothecene related SMs in this study (DON and its related compounds and NIV; see Table 2) showed that both DON and NIV levels were significantly higher in stored wheat + F. graminearum (p < 0.05) inoculum. In contrast to ZEN, DON and NIV were significantly affected by a w in terms of contamination levels, with optimum DON production at 15-20 • C and 0.95 a w . A similar trend and optimum production was observed for the DON-3-glucoside. Optimum NIV production was at the higher temperature of 25 • C.
The changes in relative amounts of enniatins produced in both stored wheat treatments are shown in Table 3. Optimum production of the four enniatins (enniatin A, A1, B, B1) was, in most cases, at 0.95 a w and 15-25 • C. With F. graminearum inoculation there appeared to be an increase in production at 15 • C and 0.90 a w which represented a significant interacting stress level.
Apicidin, aurofusarin, fusarin C, 5-hydroxyculmorin and chrysogine concentrations were higher in the wheat + F. graminearum treatments in contrast to the naturally stored wheat (Table 4). Optimum production of apicidin, aurofusarin, fusarin C and for the enniatins group was at 0.95 a w and 15-25 • C. A similar pattern was observed for moniliformin except at 15 • C where optimum production was at lower a w levels. Optimum chrysogine production was at 0.90-0.93 a w . Table 1. Production of zearalenone and related compounds in the two stored wheat grain treatments under different temperature x water activity (a w ) conditions. Red indicates maximum production levels (ng/g) while green and shades of yellow/orange represents intermediate production levels. Key: <LOD, below limits of detection. Shading is per column.  Table 3. Production of enniatins (four types and total) in the two stored wheat grain treatments under different temperature x water activity (a w ) conditions. Red indicates maximum production levels (ng/g) while green and shades of yellow/orange represents intermediate production levels. Key: <LOD, below limits of detection. Shading is per column.  Table 4. Production of Fusarium secondary metabolites (apicidin, moniliformin, aurofusarin, fusarin C, 5-hydroxyculmorin and chrysogine) in the two stored wheat grain treatments under different temperature x water activity (a w ) conditions. Red indicates maximum production levels (ng/g) while green and shades of yellow/orange represents intermediate production levels. Key: <LOD, below limits of detection. Shading is per column.

Discussion and Conclusions
This study has focused on the effect of different steady state interacting storage environmental factors which may be conducive to Fusarium and other fungal colonization and the production of a range of targeted SMs produced either by the naturally contaminating mycobiota, or that inoculated with a F. graminearum strain. This has suggested that a wide range of different fungal species related SMs are produced depending on the temperature x water stress conditions imposed, even with a low initial level of fungal contamination. Out of a total of 121 metabolites it was noticeable that approx. 30-50 metabolites were present at 15-25 • C regardless of a w conditions. At 10 • C, slower mould colonization occurs resulting in significantly less SMs being produced in both naturally contaminated stored wheat and that inoculated with F. graminearum. However, the relative ratio of the compounds produced in significant concentrations in these treatments, were significantly less. Thus, about 20-25 compounds were produced at >50 ng/g and <10 at >500 ng/g. This certainly suggests that, surprisingly, there are a wide range of SM compounds which are produced over a range of interacting environmental stress conditions at similar concentrations.
There have been few other studies of SM production under different storage conditions conducive to colonization by the natural mycobiota or the additional Fusarium inoculum. In the present study, the dry wheat grain contained ZEN, alphazol, betazol, DON, DON 3 glucoside, NIV, enniatins (4), apicidin, moniliformin, aurofusarin, fusarin C, ZEN sulphate. It was interesting that chrysogine was found to be produced in the stored grain + F. graminearum treatment and potentially an indicator of contamination, especially under water stress conditions. A recent study has identified the biosynthetic gene cluster for chrysogine (chry2-6) and has been found in F. graminearum, although predominantly present in Penicillium and Aspergillus species [10]. The production is related to nonribosomal peptide synthetases (NRPS) as deletion of these eliminated the production of chrysogine. A previous study of temperate cereals, including wheat, from different regions of Norway was carried out [11]. In wheat 20-25 compounds were found including type A (T-2; HT-2 toxin) and type B trichothecenes, ZEN and its derivatives as well as Alternaria toxins, enniatins, ergot alkaloids and some other Fusarium and Penicillium/Aspergillus SM compounds. The sample moisture contents (m.c.) were not given although they were obtained from farmers' field in an unusually different climatic season. This suggests that the wheat grain samples at harvest may have been variable in terms of moisture content influencing the wide range of compounds isolated from some of these samples.
Other studies have mainly focused on maize and targeted profiles of SM compounds [4,5]. Indeed, Adentunji et al. [4], in an extensive survey of maize samples from different climatic regions of Nigeria, examined the production of up to 60 fungal targeted SM compounds. Besides aflatoxins and fumonisins, DON and its derivatives, Alternaria toxins and a range of other fungal compounds were present. The maize samples came from different storage systems, from stores to domestic environments. This would certainly influence the m.c. of the samples and perhaps fungal colonization, influencing the types and levels of contamination found. Knowledge of the actual m.c. of the samples would have been useful, as this is a useful indication of the relative quality of maize samples. Despite this, there was a correlation (negative or positive) between storage structures and level of contamination with SMs.
Fungal SM compounds are produced in four main chemical classes: polyketides, terpenoids, shikimic acid derived compounds and non-ribosomal peptides [12]. In addition, hybrid metabolites composed of moieties from different classes are common, as in the meroterpenoids, which are fusions between terpenes and polyketides. The fungi colonizing cereals are predominantly from the ascomycetes. These often have more genes of secondary metabolism than other fungal groups such as basidiomycetes, archeo-ascomycetes and chytridiomycetes. In contrast, hemi-ascomycetes and zygomycetes have none [13]. The range of metabolites found in the wheat samples colonized by field and storage fungi in the present study suggest that physiologically the dominant fungi utilize the battery of up to 16 polyketide synthases (PKS), 10 non-ribosomal protein synthases (NRPS), 2 tryptophan synthetases (TS) and 2 dimethylallyl tryptophan synthetases (DMATS) which are critically important in SM biosynthesis. Wheat represents a very nutritious finite nutrient source and this enables the Fusarium and other colonizing fungi to produce signature enzymes that can be enriched in secondary metabolism gene clusters and responsible for the main biosynthetic steps in SM production. Previous studies of F. graminearum have certainly shown that the biosynthetic gene clusters (TRI genes) responsible for SM production are directly related to the prevailing temperature x a w environmental conditions. The activity of these groups of genes were correlated with these interacting environmental conditions and influenced the relative production of type B trichothecenes [14,15].
No ecological studies of the production of the enniatin group have been previously done. The present study suggests that in grain, optimum production of this group of compounds may occur at 20-25 • C and 0.95 a w . However, under sub-optimal conditions of temperature/a w stress can stimulate production. This has been previously shown for other mycotoxins at a molecular level where gene clusters involved in toxin production were stimulated at sub-optimal environmental conditions [14].
Furthermore, interactions between mycotoxigenic and other spoilage fungi can also influence the relative SM production patterns. Previously, interactions between F. culmorum and different wheat fungal contaminants were shown to stimulate type B trichothecene production, depending on a w x temperature conditions [16]. Previously, Magan and Aldred [17,18] suggested that fungal interactions and impacts on SM production are in a state of flux during storage depending on how external interacting environmental conditions naturally change and may further be influenced by the presence/absence of preservatives and the activity of storage pests. Thus, complex abiotic/biotic interactions may occur influencing the targeted SM production patterns.
This study has shown that efficiency of drying and subsequent storage will influence the relative colonisation of temperate cereals by a mixture of field (e.g., Fusarium, Alternaria species) and storage fungi (Penicillium, Aspergillus species). In Europe, Penicillium species, especially P. verrucosum, colonises grain post-harvest and results in contamination with ochratoxin A [19]. However, as environmental stress factors become more important it is possible that post-harvest mycotoxigenic fungi become more important pre-harvest and perhaps result in a significant change in the predominant SM compounds produced and perhaps also the relative ratio of related compounds [2].

Fungal Cultures
F. graminearum strain Fg 08/111 isolated from UK wheat and a known producer of ZEN and DON was used in this study. This was kindly supplied by Prof S. Edwards, Harper Adams University, Shropshire, U.K. Cultures of this strain were maintained in glycerol:water (67:33 v/v) at −20 • C and sub-cultured when required for experimental use.

Enumeration of Fungi
Three sub-samples (10 g) were soaked for 3 h in 90 mL of sterile distilled water supplemented with 0.1% peptone (w/v) and 0.025% (w/v) Tween 80. The samples were then homogenised for 2 min in a stomacher (Lab-Blender 400: Seward Medical, London, UK). Serial dilutions (10-10 −3 ). Aliquots (0.1 mL) from each dilution and for each sample were spread plated in triplicate on Dichloran 18% glycerol agar (DG18: CM0729; Oxoid) media and Malt extract agar (MEA: CM59; Oxoid) in 9 cm Petri plates and incubated at 25 • C for 7 days. The colonies growing on the plates were counted and their numbers expressed as colony forming units per gram sample (CFU/g dry weight sample).

Fungal Identification
One hundred wheat grains were sub-sampled. Fifty kernels were first surface-disinfected with NaOCl (0.4%) for two minutes and left to dry on sterile filter paper. Subsequently, the dry kernels were directly plated (five grains per plate) on DG18 and MEA. Petri plates were incubated at 25 • C for 7 days. The plates were then inspected visually for fungal growth with the aid of a stereo microscope [20] and the percentage of fungal genera were assessed.

Wheat Grain Moisture Content and Water Activity Adjustment
Harvested winter wheat grain in 2016 was used in these experiments. The moisture content of the wheat grain was 14.5% and the grain was stored at 4 • C until used in experiments. Initially a moisture adsorption curve was made by adding known amounts of water to 10 g sub-samples of wheat grain which was sealed in 25 mL Universal bottles, shaken vigorously and stored overnight at 4 • C. The wheat was allowed to equilibrate at 25 • C and the water activity (a w ; Aqualab 4TE, Decagon Devices, Pullman, WA, USA) and moisture content determined (oven drying at 105 • C for 17 h). The plot of added water against actual a w was used to accurately modify the wheat grain for the storage experiments.

Grain Inoculation and Incubation
Natural wheat grain sub-samples (10 g) were modified to different a w levels with water to 0.70, 0.90, 0.93 and 0.95 a w and equilibrated as detailed previously. They were placed in 40-mL vials (Chromacol Ltd, Bath, UK) with sealable caps containing a septum for gas removal. The replicates and treatments were stored in 10 L environmental chambers which also contained glycerol-water solutions (450 mL) in a beaker to maintain the equilibrium relative humidity (ERH) of the atmosphere at the target a w levels of the stored wheat samples at each experimental temperature (10,15,20 and 25 • C). Glycerol solutions were renewed weekly.
Another set of replicates (n = 4) of the wheat grain sub-samples were inoculated with four 5-mm diameter agar disk taken from a 7 day old colony of the F. graminearum strain grown on V8 agar (V8 ® , 175 mL; CaCO 3 , 3g; ZnSO 4 ·7H 2 O, 0.01 g; CuSO 4 ·5H 2 O, 0.005 g; agar, 20 g/L) and transferred to vials containing the natural wheat grain treatments and replicates and mixed thoroughly. The storage vials were kept open in the environmental storage chambers until the end of the experiment.

Sample Preparation
The natural and F. graminearum inoculated wheat grain was dried for 48 h at 60 • C and then milled and stored at 4 • C until their analyses. Five grams of milled wheat were extracted using 20 mL extraction solvent (acetonitrile/water/acetic 79/20/1 v/v/v) followed by a 1 + 1 dilution using acetonitrile/water/acetic 20/79/1 v/v/v. Five µL of the diluted extract was directly injected into the sampling port for LC-MS/MS in the equipment for analysis.

LC-MS/MS Parameters
LC-MS/MS screening of target fungal metabolites was performed with a QTrap 5500 LC-MS/MS System (Applied Biosystems, Foster City, CA, USA) equipped with a TurboIonSpray electrospray ionization (ESI) source and a 1290 Series HPLC System (Agilent, Waldbronn, Germany). Chromatographic separation was performed at 25 • C on a Gemini C 18 -column, 150 × 4.6 mm i.d., 5 µm particle size, preceded by a C 18 4 × 3 mm i.d. security guard cartridge (all from Phenomenex, Torrance, CA, USA). The chromatographic method as well as chromatographic and mass spectrometric parameters were described previously in Malachova et al. [21].