Next Article in Journal
Epigallocatechin Gallate: A Review of Its Beneficial Properties to Prevent Metabolic Syndrome
Previous Article in Journal
A Randomized, Double-Blind, Parallel Study to Evaluate the Dose-Response of Three Different Vitamin D Treatment Schemes on the 25-Hydroxyvitamin D Serum Concentration in Patients with Vitamin D Deficiency
Open AccessArticle

Modifying Choroidal Neovascularization Development with a Nutritional Supplement in Mice

1
Experimental Ophthalmology Laboratory, School of Medicine, University of Navarra, Irunlarrea s/n Street, Los Castaños Building 31008 Pamplona, Spain
2
Department of Ophthalmology, Hospital Virgen del Camino, Servicio Navarro de Salud, Irunlarrea 4 Street, 31008 Pamplona, Spain
3
IdiSNA, Navarra Institute for Health Research, 31008 Pamplona, Spain
4
Department of Ophthalmology Clínica Universidad de Navarra, University of Navarra, Avda Pio XII, 36, 31008 Pamplona, Spain
*
Author to whom correspondence should be addressed.
These authors share first authorship credit.
These authors contributed equally to this work.
Nutrients 2015, 7(7), 5423-5442; https://doi.org/10.3390/nu7075229
Received: 13 May 2015 / Revised: 5 June 2015 / Accepted: 18 June 2015 / Published: 6 July 2015
We examined the effect of nutritional supplements (modified Age Related Eye Disease Study (AREDS)-II formulation containing vitamins, minerals, lutein, resveratrol, and omega-3 fatty acids) on choroidal neovascularization (CNV). Supplements were administered alone and combined with intravitreal anti-VEGF in an early-CNV (diode laser-induced) murine model. Sixty mice were evenly divided into group V (oral vehicle, intravitreal saline), group S (oral supplement, intravitreal saline), group V + aVEGF (oral vehicle, intravitreal anti-VEGF), and group S + aVEGF (oral supplement, intravitreal anti-VEGF). Vehicle and nutritional supplements were administered daily for 38 days beginning 10 days before laser. Intravitreal injections were administered 48 h after laser. Fluorescein angiography (FA) and flat-mount CD31 staining evaluated leakage and CNV lesion area. Expression of VEGF, MMP-2 and MMP-9 activity, and NLRP3 were evaluated with RT-PCR, zymography, and western-blot. Leakage, CNV size, VEGF gene and protein expression were lower in groups V + aVEGF, S + aVEGF, and S than in V (all p < 0.05). Additionally, MMP-9 gene expression differed between groups S + aVEGF and V (p < 0.05) and MMP-9 activity was lower in S + aVEGF than in V and S (both p < 0.01). Levels of MMP-2 and NLRP3 were not significantly different between groups. Nutritional supplements either alone or combined with anti-VEGF may mitigate CNV development and inhibit retinal disease involving VEGF overexpression and CNV. View Full-Text
Keywords: antioxidants; choroidal neovascularisation; mouse model; anti-VEGF antioxidants; choroidal neovascularisation; mouse model; anti-VEGF
Show Figures

Figure 1

MDPI and ACS Style

Ivanescu, A.A.; Fernández-Robredo, P.; Heras-Mulero, H.; Sádaba-Echarri, L.M.; García-García, L.; Fernández-García, V.; Moreno-Orduna, M.; Redondo-Exposito, A.; Recalde, S.; García-Layana, A. Modifying Choroidal Neovascularization Development with a Nutritional Supplement in Mice. Nutrients 2015, 7, 5423-5442.

Show more citation formats Show less citations formats

Article Access Map by Country/Region

1
Only visits after 24 November 2015 are recorded.
Back to TopTop