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Open AccessArticle

Short-Term Effects of a High Nitrate Diet on Nitrate Metabolism in Healthy Individuals

School of Medicine and Pharmacology, University of Western Australia, Perth, WA 6000, Australia
Flinders Centre for Epidemiology and Biostatistics, Flinders University, Adelaide, SA 5042, Australia
Author to whom correspondence should be addressed.
Nutrients 2015, 7(3), 1906-1915;
Received: 31 October 2014 / Revised: 19 February 2015 / Accepted: 28 February 2015 / Published: 12 March 2015
Dietary nitrate, through the enterosalivary nitrate-nitrite-NO pathway, can improve blood pressure and arterial stiffness. How long systemic nitrate and nitrite remain elevated following cessation of high nitrate intake is unknown. In 19 healthy men and women, the time for salivary and plasma nitrate and nitrite to return to baseline after 7 days increased nitrate intake from green leafy vegetables was determined. Salivary and plasma nitrate and nitrite was measured at baseline [D0], end of high nitrate diet [D7], day 9 [+2D], day 14 [+7D] and day 21 [+14D]. Urinary nitrite and nitrate was assessed at D7 and +14D. Increased dietary nitrate for 7 days resulted in a more than fourfold increase in saliva and plasma nitrate and nitrite (p < 0.001) measured at [D7]. At [+2D] plasma nitrite and nitrate had returned to baseline while saliva nitrate and nitrite were more than 1.5 times higher than at baseline levels. By [+7D] all metabolites had returned to baseline levels. The pattern of response was similar between men and women. Urinary nitrate and nitrate was sevenfold higher at D7 compared to +14D. These results suggest that daily ingestion of nitrate may be required to maintain the physiological changes associated with high nitrate intake. View Full-Text
Keywords: high nitrate diet; nitrate metabolism; nitric oxide high nitrate diet; nitrate metabolism; nitric oxide
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Bondonno, C.P.; Liu, A.H.; Croft, K.D.; Ward, N.C.; Puddey, I.B.; Woodman, R.J.; Hodgson, J.M. Short-Term Effects of a High Nitrate Diet on Nitrate Metabolism in Healthy Individuals. Nutrients 2015, 7, 1906-1915.

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