Oral Treatment with the Extract of Euterpe oleracea Mart. Improves Motor Dysfunction and Reduces Brain Injury in Rats Subjected to Ischemic Stroke

Ischemic stroke is one of the principal causes of morbidity and mortality around the world. The pathophysiological mechanisms that lead to the formation of the stroke lesions range from the bioenergetic failure of the cells and the intense production of reactive oxygen species to neuroinflammation. The fruit of the açaí palm, Euterpe oleracea Mart. (EO), is consumed by traditional populations in the Brazilian Amazon region, and it is known to have antioxidant and anti-inflammatory properties. We evaluated whether the clarified extract of EO was capable of reducing the area of lesion and promoting neuronal survival following ischemic stroke in rats. Animals submitted to ischemic stroke and treated with EO extract presented a significant improvement in their neurological deficit from the ninth day onward. We also observed a reduction in the extent of the cerebral injury and the preservation of the neurons of the cortical layers. Taken together, our findings indicate that treatment with EO extract in the acute phase following a stroke can trigger signaling pathways that culminate in neuronal survival and promote the partial recovery of neurological scores. However, further detailed studies of the intracellular signaling pathways are needed to better understand the mechanisms involved.


Introduction
Stroke is the second leading cause of death in the world and the primary cause of disability [1] resulting in a loss of quality of life [2] and a significant burden for national and regional health systems. Ischemic stroke involves cellular and molecular events that begin with the bioenergetic failure of cells by processes that include global or focal brain hypoperfusion, excitotoxicity, oxidative stress, neuro-inflammation, blood-brain barrier dysfunction, and microvascular injuries which culminate in the death of neurons, as well as glial and endothelial cells [3][4][5].
While it is necessary to reverse ischemic stroke, tissue reperfusion can worsen the injuries by overloading the reactive oxygen species during the restoration of the blood flow [4]. In this context, many recent studies have focused on the antioxidant and antiinflammatory properties of natural products in search of complementary therapeutic strategies for the attention of ischemia-reperfusion injuries [6,7].
The work extract was reconstituted in filtered water to a concentration of 200 mg/mL, while the dosage used in the present study-200 mg/kg/day-was based on the findings of previous research [27,28].

Animals and Experimental Design
All the experimental procedures were conducted in accordance with the principles of the Brazilian National Council for the Control of Animal Experimentation (CONCEA) and were approved by the Ethics Committee on the Use of Animals of the Biological Sciences Institute of the Federal University of Pará (ICB-UFPA; CEUA No. 1225030320). All necessary precautions were taken to prevent animal suffering and distress.
For the present study, 36 adult (8-10 weeks) male Wistar rats weighing 220 g (±30 g) were obtained from the Central Animal Facility of the Biological Sciences Institute of Federal University of Pará (ICB-UFPA). These animals were housed in polypropylene cages covered by a metallic grid in a controlled environment (22 ± 2 • C; 12/12 h light/dark cycle) with ad libitum access to standard rat chow and water.
After a seven-day acclimation period, the animals were allocated randomly to one of four experimental groups (n = 9 animals/group): (i) sham, (negative control), in which the animals received only the vehicle (water); (ii) sham + EO (positive control), in which the animals received the Euterpe oleracea (EO) extract; (iii) MCAO, the animals were submitted to middle cerebral artery occlusion for 30 min and received only the vehicle; or (iv) MCAO + EO. The surgical procedures were conducted invariably between 08:00 a.m. and 11:00 a.m.
These four groups were monitored for 14 days after the surgery. The MCAO + EO animals received a dose of EO extract (200 mg/kg/day) via oral gavage 4.5 h after the beginning of the MCAO and an additional dose every day until the end of the experiment. Neurological scores were obtained every day during the experiment, with the first evaluation taking place 24 h after the first application of the EO extract, and the last record being obtained on the day following the final application. Neurological tests were conducted on the seventh and fourteenth days after the MCAO. Prior to euthanasia, blood samples were collected via cardiac puncture for biochemical analysis. After euthanasia, the brains were extracted, sectioned, and stained with cresyl violet (0.3%) and neuronal antibody ( Figure 1). cycle) with ad libitum access to standard rat chow and water.
After a seven-day acclimation period, the animals were allocated randomly to one of four experimental groups (n = 9 animals/group): (i) sham, (negative control), in which the animals received only the vehicle (water); (ii) sham + EO (positive control), in which the animals received the Euterpe oleracea (EO) extract; (iii) MCAO, the animals were submitted to middle cerebral artery occlusion for 30 min and received only the vehicle; or (iv) MCAO + EO. The surgical procedures were conducted invariably between 08:00 a.m. and 11:00 a.m.
These four groups were monitored for 14 days after the surgery. The MCAO + EO animals received a dose of EO extract (200 mg/kg/day) via oral gavage 4.5 h after the beginning of the MCAO and an additional dose every day until the end of the experiment. Neurological scores were obtained every day during the experiment, with the first evaluation taking place 24 h after the first application of the EO extract, and the last record being obtained on the day following the final application. Neurological tests were conducted on the seventh and fourteenth days after the MCAO. Prior to euthanasia, blood samples were collected via cardiac puncture for biochemical analysis. After euthanasia, the brains were extracted, sectioned, and stained with cresyl violet (0.3%) and neuronal antibody ( Figure 1).

Middle Cerebral Artery Occlusion Surgery
The MCAO surgery was conducted as described by Ferreira et al. [29]. For this process, the animals were anesthetized intraperitoneally (i.p.) with ketamine (80 mg/kg) and xylazine (10 mg/kg), then placed on a heated blanket. After the abolishment of the corneal reflex, the common carotid bifurcation was exposed through an incision to the cervical midline, revealing the internal and external carotid arteries. A silicone-coated nylon monofilament (Doccol Corp., Redlands, CA, USA) was then inserted into the stump of the external carotid artery and fed up the internal carotid artery to occlude the origin of the middle cerebral artery. The filament was withdrawn after 30 min to allow for reperfusion. The sham-operated animals were anesthetized and the carotid bifurcation exposed, but no filament was inserted. The incision was sutured, and the animals were returned to their home cages to regain consciousness, receiving dipyrone (500 mg/kg)

Middle Cerebral Artery Occlusion Surgery
The MCAO surgery was conducted as described by Ferreira et al. [29]. For this process, the animals were anesthetized intraperitoneally (i.p.) with ketamine (80 mg/kg) and xylazine (10 mg/kg), then placed on a heated blanket. After the abolishment of the corneal reflex, the common carotid bifurcation was exposed through an incision to the cervical midline, revealing the internal and external carotid arteries. A silicone-coated nylon monofilament (Doccol Corp., Redlands, CA, USA) was then inserted into the stump of the external carotid artery and fed up the internal carotid artery to occlude the origin of the middle cerebral artery. The filament was withdrawn after 30 min to allow for reperfusion. The sham-operated animals were anesthetized and the carotid bifurcation exposed, but no filament was inserted. The incision was sutured, and the animals were returned to their home cages to regain consciousness, receiving dipyrone (500 mg/kg) subcutaneously for analgesia. All the sham-operated rats survived, although mortality was 33.3% in the MCAO group (3 of the 9 animals), and 11.1% (one animal) in the MCAO + EO group.

Neurological Score and Behavioral Test
The neurological motor deficit (clinical scores) of the rats was evaluated daily after the MCAO, with the first score being recorded 24 h after first application of the EO extract and the final score being obtained on the day after the last application of the extract. Neurological deficit was assessed based on the five-point scale of Zhang et al. [30]: 0 = no neurological deficit; 1 = mild loss of contralateral forelimb muscle tone; 2 = loss of muscle tone in the contralateral forelimb of the lesion and circular movement to the side contralateral to the lesion when suspended by the tail; 3 = spontaneous circular movement to the contralateral side of the lesion; 4 = no spontaneous motor activity. The animals with a score of 4 were euthanized to avoid suffering and distress. The observer that evaluated the rats was blind to the treatments.

Histological Analyses
After euthanasia, the animals were perfused transcardially with phosphate buffer saline (PBS, pH 7.4; 4 • C) and then with 4% formaldehyde (pH 7.4). The brain was then extracted from the skull and fixed in 4% formaldehyde for 72 h, followed by cryoprotection in 30% sucrose for a further 72 h before being cut into serial coronal sections (40 µm) in a Leica 1850 UV semi-automatic cryostat (Leica Microsystems, Wetzlar, Germany).
The sections were mounted on gelatin-coated microscope slides, air-dried, and then stained with cresyl violet (0.3%) to measure the percentage area of cerebral infarction [31]. The sections were then dehydrated in an increasing ethanol series, cleared in xylene, and coverslipped with Entellan (Merck, Danstadt, Germany). The images were captured using a Toshiba digital camera (Toshiba America Inc., NY, USA), and the percentage of infarction area (PIA) was calculated using the formula [29]: PIA = (area of infarction/area of the ipsilateral hemisphere) × 100.
The neuronal cells were immunostained overnight at 4 • C using the free-floating method with the anti-NeuN mouse primary antibody (1:2000, Merck-Millipore, Darmstadt, Germany). After washing with phosphate buffered saline (PBS), a DAKO EnVi-sionTM + Dual Link System-HRP kit (Carpinteria, CA, USA) was used according to the manufacturer's protocol. This system is based on an HRP-labeled polymer which is conjugated with secondary antibodies. After washing with PBS, DAB (3,3 -diaminobendine; Sigma-Aldrich) was used as a chromogen to stain the sections. Finally, the sections were mounted on gelatin-coated microscope slides, air-dried, dehydrated in an increasing ethanol series, cleared in xylene, and coverslipped with DPX mounting medium. The images were captured using a Toshiba digital camera (Toshiba America Inc., NY, USA) and analyzed with the Stereoinvestigator (MBF Bioscience, Williston, VT, USA) and ImageJ software (NIH, Bethesda, MD, USA). The NeuN + cells were counted in eight sections from the lesion and peri-infarction area in the somatosensory cortex, and the striatum ipsilateral to the area of the lesion in sample fields of 200 µm × 200 µm, separated by cortical layers, with n = 6 animals per group [32].

Biochemical Analysis
For the biochemical assays, the serum was extracted from the blood samples (obtained by cardiac puncture) by centrifugation at 3000 rpm for 10 min. The following nine biochemical parameters were determined using a Wiener CM200 chemical analyzer, following the manufacturer's instructions: aspartate aminotransferase (AST), alanine aminotransferase (ALT), urea (URE), creatinine (CRE), high-density lipoprotein (HDL), very low-density lipoprotein (VLDL), low-density lipoprotein (LDL), total cholesterol (CHO), and triglyceride (TRY). All the analyses were run in the ICB-UFPA Laboratory of Clinical Analyses (LAC).

Statistical Analyses
Prior to the analyses, the normality of the variances was verified using the Kolmogorov-Smirnov test. The data are presented as the mean with the standard deviation (SD), and the F and p values included whenever relevant. A p < 0.05 significance level was considered for all the analyses. Differences between pairs of groups were analyzed using Student's t test, while those among three or more groups were evaluated using a two-way Analysis of Variance (ANOVA), followed by Tukey's post hoc test for pairwise comparisons. The data were analyzed using GraphPad Prism, version 9 (Graph-Pad Software Inc., San Diego, CA, USA).

Açaí Extract Improves the Behavioral Outcome of Animals Submitted to Ischemic Stroke
The neurological scores varied significantly among treatment groups and days (F (39, 392) = 2.419; p < 0.0001; Figure 2). The comparison of the days among treatment groups (F (3, 392) = 1996; p < 0.0001) showed that there was no significant variation between the sham and sham + EO groups (control groups) over the course of the experiment (p > 0.9999). The neurological scores of the ischemic animals (MCAO groups) were significantly different from those of the control groups (p < 0.0001, for all comparisons). The animals of the MCAO group presented high rates of spontaneous circular movement to the contralateral side of the lesion (score 3), while treatment with EO extract (MCAO + EO group) alleviated the neurological dysfunction significantly in comparison with the MCAO group from the ninth day (D9) onward (D9 and D10: p = 0.0287; D11 and D12: p = 0.0021; D13 and D14: p < 0.0001; for MCAO vs. MCAO + EO groups), with the rats presenting a loss of muscle tone in the contralateral forelimb and circular movements to the contralateral side when suspended by the tail (score 2).
Analysis of Variance (ANOVA), followed by Tukey's post hoc test for pairwise comparisons. The data were analyzed using GraphPad Prism, version 9 (Graph-Pad Software Inc., San Diego, CA, USA).

Açaí Extract Improves the Behavioral Outcome of Animals Submitted to Ischemic Stroke
The neurological scores varied significantly among treatment groups and days (F (39, 392) = 2.419; p < 0.0001; Figure 2). The comparison of the days among treatment groups (F (3,392) = 1996; p < 0.0001) showed that there was no significant variation between the sham and sham + EO groups (control groups) over the course of the experiment (p > 0.9999). The neurological scores of the ischemic animals (MCAO groups) were significantly different from those of the control groups (p < 0.0001, for all comparisons). The animals of the MCAO group presented high rates of spontaneous circular movement to the contralateral side of the lesion (score 3), while treatment with EO extract (MCAO + EO group) alleviated the neurological dysfunction significantly in comparison with the MCAO group from the ninth day (D9) onward (D9 and D10: p = 0.0287; D11 and D12: p = 0.0021; D13 and D14: p < 0.0001; for MCAO vs. MCAO + EO groups), with the rats presenting a loss of muscle tone in the contralateral forelimb and circular movements to the contralateral side when suspended by the tail (score 2).
Clinical improvement was observed in the MCAO + EO group during the course of the treatment (Supplementary Table S1), with days D9 and D10 being significantly different in comparison with D1 and D2 (p < 0.01), and D11-D12 significantly different from D1-D3 (p < 0.01). Days D13-D14 were also significantly different from each of the five first days of the treatment, i.e., D1-D5 (p < 0.01). Neurological/motor deficit scores of the animals submitted to middle cerebral artery occlusion (MCAO) and treated with clarified Euterpe oleracea extract. The data are presented as the mean ± SD (n = 6-9 animals/group; two-way ANOVA followed by Tukey's post hoc test; * p < 0.05).
Clinical improvement was observed in the MCAO + EO group during the course of the treatment (Supplementary Table S1), with days D9 and D10 being significantly different in comparison with D1 and D2 (p < 0.01), and D11-D12 significantly different from D1-D3 (p < 0.01). Days D13-D14 were also significantly different from each of the five first days of the treatment, i.e., D1-D5 (p < 0.01).

Açaí Extract Reduces the Size of the Area of Infarction and Increases the Number of Surviving Neurons after Ischemic Stroke
The behavioral changes observed in the ischemic animals are consistent with lesions in the cortical and subcortical regions and indicate attenuation of the damage by treatment with the EO extract. The ischemic animals presented cortical-subcortical injuries with damage in the primary and secondary somatosensory areas and in the striatum, with a mean area of hemispheric lesion of 31.37 ± 13.32% (MCAO group). After 14 days of EO treatment (MCAO + EO group), the mean area affected was reduced to values 15.02 ± 5.39% (p = 0.0217). No lesions were observed in any of the animals of the two control groups (Figure 3). damage in the primary and secondary somatosensory areas and in the striatum, with a mean area of hemispheric lesion of 31.37 ± 13.32% (MCAO group). After 14 days of EO treatment (MCAO + EO group), the mean area affected was reduced to values 15.02 ± 5.39% (p = 0.0217). No lesions were observed in any of the animals of the two control groups (Figure 3). Cerebral ischemia culminates in cell death, mainly of neurons in the cortical region, which was the reason the NeuN + cell count was conducted per cortical layer ( Figure 4A). As no difference was found between the animals of the two control groups (sham and sham + EO), it appears that the administration of the EO extract does not alter the density of the cortical neurons or induce cell death ( Figure 4B-G; Supplementary Table S2).
However, the MCAO did cause neuronal death, as indicated by the significant decrease in the number of NeuN + cells in layers II/III through VI (Figure 4B-G; Supplementary Table S2) of the cortical region of the somatosensory cortex (Figures 3 and 4A). Surprisingly, the animals that received EO extract for 14 days presented significant levels of preservation in the number of NeuN + cells in these same layers ( Figure 4B-G; Supplementary Table S2). This result indicates that the EO treatment may activate neuronal survival pathways.
The area adjacent to the ischemic lesion, known as the penumbra, is also of considerable importance, in particular because it is a potentially recoverable area. In the present study, treatment with the EO extract was reflected in a significantly higher number of NeuN + cells in the treated ischemic animals (MCAO + EO group) in comparison with the MCAO group ( Figure 4H, Supplementary Table S2; p < 0.01). The number of NeuN + cells Cerebral ischemia culminates in cell death, mainly of neurons in the cortical region, which was the reason the NeuN + cell count was conducted per cortical layer ( Figure 4A). As no difference was found between the animals of the two control groups (sham and sham + EO), it appears that the administration of the EO extract does not alter the density of the cortical neurons or induce cell death ( Figure 4B-G; Supplementary Table S2).
However, the MCAO did cause neuronal death, as indicated by the significant decrease in the number of NeuN + cells in layers II/III through VI (Figure 4B-G; Supplementary  Table S2) of the cortical region of the somatosensory cortex (Figures 3 and 4A). Surprisingly, the animals that received EO extract for 14 days presented significant levels of preservation in the number of NeuN + cells in these same layers (Figure 4B-G; Supplementary Table S2). This result indicates that the EO treatment may activate neuronal survival pathways.
The area adjacent to the ischemic lesion, known as the penumbra, is also of considerable importance, in particular because it is a potentially recoverable area. In the present study, treatment with the EO extract was reflected in a significantly higher number of NeuN + cells in the treated ischemic animals (MCAO + EO group) in comparison with the MCAO group ( Figure 4H Table S2; p < 0.01), indicating that the treatment with the EO extract contributed significantly to the preservation of the cells in this region. These findings indicate that the EO treatment offers important protective effects.
of the striatum was also reduced significantly in the animals of the MCAO group ( Figure  4I, Supplementary Table S2; p < 0.01), indicating that the treatment with the EO extract contributed significantly to the preservation of the cells in this region. These findings indicate that the EO treatment offers important protective effects. . The data are expressed as the mean ± SD (n = 6 animals per group; C-H, two-way ANOVA followed by Tukey's post hoc test; I, Student's t test; * p < 0.05; ** p < 0.01; *** p < 0.001).

Açaí Extract Did Not Cause Biochemical Alterations after Ischemic Stroke
No significant variation was found in the biochemical parameters of the animals treated with EO extract following ischemic stroke ( Figure 5). . The data are expressed as the mean ± SD (n = 6 animals per group; C-H, two-way ANOVA followed by Tukey's post hoc test; I, Student's t test; * p < 0.05; ** p < 0.01; *** p < 0.001).

Açaí Extract Did Not Cause Biochemical Alterations after Ischemic Stroke
No significant variation was found in the biochemical parameters of the animals treated with EO extract following ischemic stroke ( Figure 5).

Discussion
The present study is the first to demonstrate that the extract of Euterpe oleracea may attenuate the progression of the damage caused by cerebral ischemia, as demonstrated by the improvement of neurological function, the reduction of ischemic area, and the decrease in neuronal death. We also showed that the application of the extract did not alter either the lipid profile or the liver and kidney functions of the experimental animals. This is an important point because the application does not limit therapy in patients with hepatic or renal impairments.
In most experimental studies, the effects of anthocyanins with antioxidant and antiinflammatory properties have been shown to attenuate injuries caused by ischemia and reperfusion [33][34][35][36]. The literature elucidates the fact that polyphenols found in EO extract have positive effects on modulating oxidative and inflammatory activity in in vivo and in vitro models by decreasing reactive oxygen species, increasing antioxidant activity, and regulating inflammatory mediators [37,38]. Furthermore, these effects have also been found in humans submitted to ingestion of the EO extract consisting of polyphenolic compounds such as cyanidin-3-glycoside and cyanidin-3-rutinoside, as well as monomeric catechin and epicatechin or oligomeric procyanidins [39,40].
Shin et al. [33] showed that, after 24 h of reperfusion, pretreatment with anthocyanins extracted from the bilberry (300 mg/kg of anthocyanins) enabled a reduction in the size of the cerebral infarction, a finding which may have been related to the suppression of the JNK and p53 signaling pathways. Cui et al. [35] demonstrated that the pretreatment of mice with Myrica rubra, whose principal anthocyanin is cyanidin-3-O-glucoside, for seven days prior to the MCAO, was effective for the reduction of both damage to neurological function and the volume of the cerebral infarction, principally in the middle-and highdose groups (150 and 300 mg/kg).

Discussion
The present study is the first to demonstrate that the extract of Euterpe oleracea may attenuate the progression of the damage caused by cerebral ischemia, as demonstrated by the improvement of neurological function, the reduction of ischemic area, and the decrease in neuronal death. We also showed that the application of the extract did not alter either the lipid profile or the liver and kidney functions of the experimental animals. This is an important point because the application does not limit therapy in patients with hepatic or renal impairments.
In most experimental studies, the effects of anthocyanins with antioxidant and antiinflammatory properties have been shown to attenuate injuries caused by ischemia and reperfusion [33][34][35][36]. The literature elucidates the fact that polyphenols found in EO extract have positive effects on modulating oxidative and inflammatory activity in in vivo and in vitro models by decreasing reactive oxygen species, increasing antioxidant activity, and regulating inflammatory mediators [37,38]. Furthermore, these effects have also been found in humans submitted to ingestion of the EO extract consisting of polyphenolic compounds such as cyanidin-3-glycoside and cyanidin-3-rutinoside, as well as monomeric catechin and epicatechin or oligomeric procyanidins [39,40].
Shin et al. [33] showed that, after 24 h of reperfusion, pretreatment with anthocyanins extracted from the bilberry (300 mg/kg of anthocyanins) enabled a reduction in the size of the cerebral infarction, a finding which may have been related to the suppression of the JNK and p53 signaling pathways. Cui et al. [35] demonstrated that the pretreatment of mice with Myrica rubra, whose principal anthocyanin is cyanidin-3-O-glucoside, for seven days prior to the MCAO, was effective for the reduction of both damage to neurological function and the volume of the cerebral infarction, principally in the middle-and high-dose groups (150 and 300 mg/kg). The neurological scores of the ischemic animals treated with EO extract improved significantly from the ninth day onward. Sunil et al. [34] found that the total oligomeric flavonoids of Cyperus rotundus (200 mg/kg, by oral gavage), a traditional Indian herb from the Ayurvedic medicine system, when administered to rats once a day for one week (including four days prior to the MCAO and three days afterward), alleviated the neurological deficit significantly in comparison with untreated ischemic rats. Fu et al. [36] also showed that a high dose (250 or 500 mg/kg) of proanthocyanidins extracted from grape seed improved the neurological score and decreased the area of infarction in comparison with the untreated MCAO group.
Based on this evidence, our findings indicate that both the behavioral improvement and the reduction of the area of cerebral infarction may be related to the presence of anthocyanins (primarily cyanidin-3-glucoside and cyanidin-3-rutinoside) and non-anthocyanin flavonoids (homoorientin and orientin), which are the principal compounds of the clarified EO extract applied in the present study [14,20,25,26]. We also showed that the ischemic animals presented an important motor deficit and that the therapy with EO extract reduced this dysfunction, in particular after one week of treatment. The neurological deficits exhibited by the animals in the present study correlated with the brain region affected by the MCAO which caused injuries in areas that are important for motor processing, such as the somatosensory cortex and the striatum [32]. We also provided the first evidence that the ingestion of the EO extract at a dose of 200 mg/kg/day reduced the area of infarction and contained the neuronal damage. However, one of the limitations of the present study is the lack of evaluation of other motor parameters affected by stroke, such as balance and walking. Thus, more behavioral tests are needed to measure the potential benefit of EO extract in clinical improvement.
Sensorial information is known to have a strong influence on motor processing and is essential for rehabilitation, especially following ischemic stroke [41,42]. Previous studies have shown that changes in the connections between the motor and somatosensory cortexes occur primarily during the acute phase of the stroke. This finding is extremely important because therapies initiated in the acute phase present greater benefits than those initiated later on [43][44][45].
Some studies have also demonstrated that the repair of neuronal connections following ischemic stroke depends on the cellular tissue adjacent to the infarcted area and on other factors, such as glial activation [46,47]. It is also important to note that the motor cortex is able to receive signals directly from the somatosensory cortex, although we cannot rule out the possibility that connections are mediated through the thalamus, especially its posterior medial portion [32,48].
In studies of rodents, these reciprocal connections maintained between the somatosensory and motor cortexes are innervated primarily by cortical layers II/III and V [49,50]. The present study showed a significant level of preservation of the neurons in these layers in the ischemic animals treated with EO extract, indicating that this neuroprotection may be related to the improvement in behavior. From this perspective, the partial integrity of these cortical layers may be important for the maintenance of the afferents/efferents connecting the subcortical and neocortical regions, such as the basal ganglia and the thalamic control centers [51]. This relationship reinforces the importance of the cortical neuroprotection that appeared to be provided by the use of the EO extract.
One other important finding of the present study is that the use of EO extract for 14 days did not appear to alter the lipid homeostasis or metabolism and excretion pathways, such as liver and kidney functions. A number of previous studies have provided evidence of the significant therapeutic potential of compounds extracted from açaí for the control of the lipid profile and improvement of atherosclerosis, in addition to the prevention of hepatic steatosis, although this potential is dose-dependent and also affected by the treatment time [52,53].
It is nevertheless important to note that the pathophysiology of ischemic strokeinduced brain lesions is complex and multifactorial. The blockage of blood flow in the principal artery has a rapid effect on the neurons, which die by necrosis forming the nucleus of the ischemia (core of the lesion). However, the peripheral neurons may be supplied by arteries peripheral to the lesion and may undergo late apoptosis if no neuroprotective therapy is implemented [54]. Acute arterial occlusion leads to bioenergetic failure which occurs through oxygen and glucose deprivation (OGD), the loss of ionic homeostasis, excitotoxicity, mitochondrial dysfunction, and the generation of reactive oxygen species (ROS) in the neurons ( Figure 6). This process may also lead to the activation of inflammatory cells that release cytokines, leading to an increase in inflammatory processes that may also be responsible for late apoptosis and the expansion of the area of the lesion [54,55]. Stroke triggers cell death by necrosis (acute) and apoptosis/neurodegeneration (late). More studies are therefore needed to better characterize signaling pathways where EO extract can act in the control and attenuation of apoptotic processes, as well as the antioxidant pathways that may be activated and result in neuronal survival. therapy is implemented [54]. Acute arterial occlusion leads to bioenergetic failure wh occurs through oxygen and glucose deprivation (OGD), the loss of ionic homeostasis, citotoxicity, mitochondrial dysfunction, and the generation of reactive oxygen spe (ROS) in the neurons ( Figure 6). This process may also lead to the activation of inflam tory cells that release cytokines, leading to an increase in inflammatory processes that m also be responsible for late apoptosis and the expansion of the area of the lesion [54, Stroke triggers cell death by necrosis (acute) and apoptosis/neurodegeneration (la More studies are therefore needed to better characterize signaling pathways where extract can act in the control and attenuation of apoptotic processes, as well as the ant idant pathways that may be activated and result in neuronal survival. As observed in previous studies, the clarified EO extract is rich in these compou [14,20,25,26]. Given this fact, one of the probable therapeutic targets of the EO ext would likely be the reduction in the formation of reactive oxygen species, helping to av DNA damage in addition to reducing the oxidative stress caused by glutamate excito icity and mitochondrial damage. The process would improve the inflammatory sta through the control of the reactive glia. This is one of the possible hypotheses that m explain the relief of stroke damage by the EO extract ( Figure 6). From this perspect studies involving reactive oxygen species generation, glial reactivity, and inflammat biomarkers are needed to understand the mechanisms modulated by EO extract c pounds on inflammatory and oxidative activity in a model of cerebral ischemia. As observed in previous studies, the clarified EO extract is rich in these compounds [14,20,25,26]. Given this fact, one of the probable therapeutic targets of the EO extract would likely be the reduction in the formation of reactive oxygen species, helping to avoid DNA damage in addition to reducing the oxidative stress caused by glutamate excitotoxicity and mitochondrial damage. The process would improve the inflammatory status through the control of the reactive glia. This is one of the possible hypotheses that may explain the relief of stroke damage by the EO extract ( Figure 6). From this perspective, studies involving reactive oxygen species generation, glial reactivity, and inflammatory biomark-ers are needed to understand the mechanisms modulated by EO extract compounds on inflammatory and oxidative activity in a model of cerebral ischemia.

Conclusions
Overall, the findings of the present study indicate clearly that treatment with EO extract in the acute phase following a stroke may trigger signaling pathways that culminate in neuronal survival and contribute to a partial recovery of the clinical scores of the animals. Even so, more systematic studies of the intracellular signaling pathways involved in this process will be needed to better understand its mechanisms.
Supplementary Materials: The following supporting information can be downloaded at: https://www. mdpi.com/article/10.3390/nu15051207/s1, ST1 (Supplementary Table S1)-Neurological/motor deficit scores of animals submitted to middle cerebral artery occlusion (MCAO) and treated with clarified Euterpe oleracea (EO) extract. Data are presented as means ± SD (n = 6-9 animals per group). * p < 0.05 vs. sham and sham + EO. # p < 0.05 vs. MCAO. Different letters above the data points denote significant differences between days (column) (p < 0.05). ST2 (Supplementary Table S2)-Neuronal death attenuated by clarified Euterpe oleracea (EO) extract after middle cerebral artery occlusion (MCAO). Data are presented as means ± SD (n = 6 animals per group).* p < 0.05 vs. sham and sham + EO. # p < 0.05 vs. MCAO.  Institutional Review Board Statement: All the experimental procedures were conducted in accordance with the principles of the Brazilian National Council for the Control of Animal Experimentation (CONCEA) and were approved by the Ethics Committee on the Use of Animals of the Biological Sciences Institute of the Federal University of Pará (ICB-UFPA; CEUA No. 1225030320). All necessary precautions were taken to prevent animal suffering and distress.

Data Availability Statement:
The raw data supporting the conclusions of this article will be made available by the authors, without undue reservation.