The Effect of an Essential Oil Blend on Growth Performance, Intestinal Health, and Microbiota in Early-Weaned Piglets

Essential oils (EO) are promising feed additives for their antibacterial, antioxidant, and immune-enhancing abilities with low toxicity. Carvacrol, thymol, and cinnamaldehyde are commonly used to synthesize EO. However, few studies focus on combining these three EO in early-weaned piglets. In the present study, 24 piglets weaned at 21 d of age were randomly divided into 2 groups (6 replicate pens per group, 2 piglets per pen). The piglets were fed a basal diet (the control group) and a basal diet supplemented with 400 mg/kg EO (a blend consisting of carvacrol, thymol, and cinnamaldehyde, the EO group) for 28 days. At the end of the experiment, one piglet per pen was randomly chosen to be sacrificed. Growth performance, hematology, plasma biochemical indices, antioxidant capacity, intestinal epithelial development and immunity, colonic volatile fatty acids (VFA), and microbiota were determined. The results indicated that the diet supplemented with EO significantly improved average daily feed intake (ADFI, p < 0.01) and average daily gain (ADG, p < 0.05) in the day 0 to 28 period. EO supplementation led to a significant decrease in plasma lysozyme (p < 0.05) and cortisol levels (p < 0.01). Additionally, EO significantly promoted jejunal goblet cells in the villus, jejunal mucosa ZO-1 mRNA expression, ileal villus height, and ileal villus height/crypt depth ratio in piglets (p < 0.05). The ileal mucosal TLR4 and NFκB p-p65/p65 protein expression were significantly inhibited in the EO group (p < 0.05). Colonic digesta microbiota analysis revealed that bacteria involving the Erysipelotrichaceae family, Holdemanella genus, Phascolarctobacterium genus, and Vibrio genus were enriched in the EO group. In conclusion, these findings indicate that the EO blend improves ADG and ADFI in the day 0 to 28 period, as well as intestinal epithelial development and intestinal immunity in early-weaned piglets, which provides a theoretical basis for the combined use of EO in weaned piglets.


Introduction
Early weaning is a universal technique to improve the economic benefit of pig farms. However, early weaning is also one of the most stressful events throughout a pig's life [1]. Separation of piglets from sows, abrupt transition in diet, increased exposure to pathogens, transportation, new environment, fighting, and social hierarchy stress all contribute to weaning stress [1][2][3][4][5]. Weaning stress leads to structural and functional changes in the intestine, including shorter villus, deeper crypt, impaired barrier function, damaged absorptive capacity, and decreased digestive enzyme activities [6,7]. Further, weaning stress potentiates an impaired antioxidant system and increased inflammation, leading to diarrhea and reduced growth [8][9][10]. Nutritional intervention is an effective and convenient method to alleviate weaning stress. However, effective feed additives, including antibiotics and zinc oxide, are confined in their use, considering bacterial resistance and environmental pollution. Thus, new feed additives that are safer and more environmentally friendly are needed.
Essential oils (EO) are aromatic, volatile oil liquids extracted from plants [11]. EOs possess antibacterial, antioxidant, and immune-enhancing abilities with low toxicity, residue, and pollution [12]. Thus, EOs are promising feed additives to alleviate piglet weaning stress as a substitute for antibiotics. Carvacrol, thymol, and cinnamaldehyde are wildly used EO constituents in animal husbandry. Carvacrol and thymol are phenolic monoterpenoids and cinnamaldehyde is a type of phenylpropene found in plant EOs [13][14][15]. The primary function of these EOs is antibacterial activity via interfering with bacterial cytoplasmic membranes and energy metabolism [15]. Additionally, carvacrol, thymol, and cinnamaldehyde are reported to possess antioxidant, antifungal, anticancer, anti-inflammatory, and antidiabetic capacities [16][17][18][19]. The combined use of these EOs has been explored to exert their potential. Reportedly, the combination of cinnamaldehyde and thymol could improve growth performance, digestibility of nutrients, immunity, total antioxidant capacity, and the development of jejunal mucosa in weaned piglets [20][21][22]. The combination of thymol and carvacrol has contributed to increased growth performance, digestibility, and duodenal villus height in weaned piglets [23]. As for the combination of carvacrol, thymol, and cinnamaldehyde, a previous study revealed that mixed EOs led to increased oxidative stress, nitrogen utilization, and decreased odor emission in weaning piglets [24]. It seems contradictory that the combination of the three EOs harms the piglets. However, the effect of EOs depends largely on the species and concentration. The effect of the combination of these three EOs on early-weaned piglets needs further studies.
Therefore, the present study investigated the effect of the EO blend (a combination of carvacrol, thymol, and cinnamaldehyde) on growth performance, immunity, intestinal epithelial development, and intestinal flora in early-weaned piglets.

Experimental Design
A total of 24 piglets (Duroc × Landrace × Yorkshire, 7.57 ± 0.25 kg) weaned at 21 d of age were randomly assigned to 2 groups (6 replicate pens per group, 2 piglets per pen). They were fed either a basal diet (control group) or a basal diet supplemented with 400 mg/kg EO (EO group). Several EO concentrations were chosen in the pre-experiment (Supplementary Table S1) based on the previous study [24], and the 400 mg/kg group was selected for further analysis for its great effect on average daily gain (ADG). The composition and nutrient levels of the basal diet are shown in Table 1 and met the nutrition requirements recommended by the National Research Council [25]. EO used in the present study were provided by Kemin (China) Technologies Co., Ltd., Zhuhai, China (Cinsential TM Dry, the product contains 2.64% carvacrol, 1.34% thymol, and 13.80% cinnamaldehyde). For adaptation, a basal diet was provided to all piglets for 3 d before the experiment. Piglets were housed in pens with plastic slotted floors, feeders, and nipple drinkers. All piglets had free access to feed and water. The experiment was divided into two stages: 1 d to 10 d and 11 d to 28 d.

Sample Collection
At the end of the experiment, one piglet per pen was randomly chosen for sampling. After overnight fasting, piglets were euthanized with an intravenous injection of 4% sodium pentobarbital solution (40 mg/kg body weight; Sigma, St. Louis, MO, USA) [26]. Before sacrifice, the blood sample (EDTA and heparin) was collected from the anterior vena cava of piglets. The heparin samples were centrifuged at 3000× g at 4 • C for 10 min to obtain plasma and were then frozen in aliquots. The heart, liver, spleen, and kidney were obtained and weighed. Segments of the jejunum and ileum were fixed in 4% paraformaldehyde. Further, jejunal and ileal mucosa cell layers were scraped off, snap-frozen in liquid nitrogen, and stored at −80 • C. Colonic digesta was collected, frozen, and stored at −80 • C.

Growth Performance, Fecal Score, and Organ Index
Body weight was measured every 2 weeks, and feed intake was recorded every day for the calculation of ADG, average daily feed intake (ADFI), and feed efficiency (feed/gain, F/G). Diarrhea was evaluated daily by observers blinded to the treatment using fecal scores (0, normal, firm feces; 1, possible slight diarrhea; 2, definitely unformed, moderately fluid feces; 3, very watery and frothy diarrhea) for each pen [27]. Organ indexes were calculated as the percentage of body weight.

Phenotype of the T-Lymphocytes
Whole EDTA blood was used for T-lymphocytes phenotype analysis following the procedures based on a previous study [28]. Subpopulations were specified using antibodies against CD3, CD4, and CD8 (Pig CD3e PE, Pig CD8a Alexa 647, and Pig CD4a PerCP-Cy5.5 were all from BD Biosciences, New York, NY, USA). After erythrocytes were lysed using erythrocyte lysate (CwBio, Beijing, China), samples were centrifuged at 450× g at 4 • C for 10 min and suspended with PBS to obtain leukocytes. Leukocytes were labeled using antibodies for 30 min on the ice. Single-labeled and non-labeled samples were used as controls. At least 10,000 cells were detected per sample using a Beckman MoFlo XDP flow cytometer (Beckman, Germany).

Intestinal Morphology
Hematoxylin-eosin staining was applied to observe jejunal and ileal morphology. Alcian blue and periodic acid-Schiff staining was applied to count goblet cells [29]. Fixed samples were dehydrated, paraffin-embedded, sectioned, and stained as described in a previous study [30]. Images were acquired with 100 × magnification using an Olympus BX51 microscope (Olympus, Japan). Villus height, crypt depth, and goblet cell numbers were evaluated using the Image-Pro Plus 6.0 image processing and analysis system [31]. Five randomly selected fields were selected for villus height and crypt depth analysis. Goblet cells were counted manually in 10 complete villi or crypts.

Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)
RNA extraction and RT-qPCR were conducted according to a previous study [32]. Briefly, total RNA was isolated from jejunal and ileal mucosa using AG RNAex Pro Reagent (Accurate Biotechnology [Hunan] Co., Ltd., Changsha, China). The concentration and quality of RNA were assessed using NanoDrop ND-2000 Spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA) and the 1% agarose electrophoresis. First-strand synthesis of complementary DNA was performed using the Evo M-MLV RT Kit with gDNA Clean for qPCR (Accurate Biotechnology [Hunan] Co., Ltd.). Primers used in RT-qPCR were designed using the NCBI online primer design tool (Primer-BLAST: http://www.ncbi.nlm.nih.gov/tools/primerblast/ (accessed on 1 July 2021)) according to the gene sequence of the pig or selected from published references (Supplementary Table S2). The RT-qPCR was performed using SYBR Green Premix Pro Taq HS qPCR Kit (Accurate Biotechnology [Hunan] Co., Ltd.) on a LightCycler480 Real-Time PCR system (Roche Diagnostics, Germany). The relative gene expression was normalized by GAPDH using the 2 −∆∆Ct method [33]. Data were displayed as the relative values to the control group.

Western Blotting Analysis
The ileal mucosa samples were homogenized with RIPA buffer and the total protein concentration was determined using the BCA method following the manufacturer's instructions (Beyotime Institute of Biotechnology). The protein expression was detected following the procedures described previously [34]. The antibodies used in the present study were as follows: nuclear factor kappa B (NF-κB) p65 ( . The protein content was normalized to β-actin, and data were displayed as the relative values to the control group.

Determination of Volatile Fatty Acids (VFA)
VFA concentration in colonic digesta was determined. Briefly, frozen digesta were defrosted, and approximately a 1.00 g sample was taken and weighed. Samples were mixed thoroughly with ddH 2 O and centrifuged at 13,751× g for 10 min to obtain the supernatant. The supernatant was mixed with 25% metaphosphoric acid solution (9:1, vol/vol). The mixture was incubated at room temperature for 4 h and filtered with a 0.45µm polysulfide membrane. The VFA concentration was determined using an Agilent 7890A gas chromatograph coupled with an Agilent 5975C mass spectrometer (Agilent Technologies, Santa Clara, CA, USA).

Colonic Digesta Microbiota Analysis
Microbiota analysis was conducted as described in our previous study [35]. Bacterial DNA from colonic content was extracted and amplified using specific primers with barcodes (16S V3 + V4). Illumina NovaSeq PE250 platform (Illumina, San Diego, CA, USA) was used to conduct paired-end sequencing. Raw tags were assembled and filtered to obtain clean data using fqtrim (version 0.9.4) and Vsearch (version 2.3.4). Sequences were assigned to the same operational taxonomic units (OUT) at a 97% similarity level using the UPARSE (version 7.0.1001) [36]. Alpha and beta community diversity was determined using the QIIME2.

Statistical Analysis
Statistical analysis and diagram visualization were completed using GraphPad Prism (version 8.0.2) and R software (version 3.5.2). The student's unpaired T-test was used to assess differences between the two groups after the normality of data was evaluated with the Shapiro-Wilk W-test and the potential outliers were evaluated with ROUT analysis. Data were expressed as means ± standard error of the mean (SEM), 0.05 < p < 0.1 indicated a trend toward significance, 0.01 ≤ p < 0.05 was considered significant, and p < 0.01 was considered extremely significant.

Effect of EO on Piglet Growth Performance
The effects of dietary supplementation of EO on the growth performance of weaned piglets are shown in Table 2. Compared to the control group, the ADG from 0 to 28 d (p < 0.05) and the ADFI during different periods (p < 0.05) were significantly higher in the EO group. Additionally, the piglets fed the EO diet had a higher body weight on day 28 (p < 0.1). However, the body weight and F/G were comparable between the two groups (p > 0.05).

Effect of EO on Piglet Diarrhea Score and Organ Index
The diarrhea score for the entire experiment period was calculated. There was no significant difference in diarrhea score and organ index between the two groups (p > 0.05, Figure 1). 0.1). However, the body weight and F/G were comparable between the two groups (p > 0.05).

Effect of EO on Piglet Diarrhea Score and Organ Index
The diarrhea score for the entire experiment period was calculated. There was no significant difference in diarrhea score and organ index between the two groups (p > 0.05, Figure 1).

Effect of EO on Piglet Hematology, Plasma Biochemical Index, and Antioxidant Capacity
There was no significant difference in the hematology index between the two groups (p > 0.05, Supplementary Table S3). EO supplementation significantly decreased the plasma cholesterol and HDL-C level (p < 0.05) compared to the control group ( Figure 2B,D). The plasma LDL-C in the EO group was lower than in the control group (p < 0.1, Figure 2C). Additionally, there was no significant difference in plasma MDA level, SOD activity, GSH-Px activity, and T-AOC between the two groups (p > 0.05; Figure 2E-H).

Effect of EO on Piglet Hematology, Plasma Biochemical Index, and Antioxidant Capacity
There was no significant difference in the hematology index between the two groups (p > 0.05, Supplementary Table S3). EO supplementation significantly decreased the plasma cholesterol and HDL-C level (p < 0.05) compared to the control group ( Figure  2B,D). The plasma LDL-C in the EO group was lower than in the control group (p < 0.1, Figure 2C). Additionally, there was no significant difference in plasma MDA level, SOD activity, GSH-Px activity, and T-AOC between the two groups (p > 0.05; Figure 2E-H).

Effect of EO on Piglet Immunity
Compared to the control group, the plasma cortisol (p < 0.01) and lysozyme (p < 0.05) levels were significantly decreased in the EO group ( Figure 3D,E). There was no significant difference in blood lymphocyte subsets between these two groups ( Figure 3F-H).

Effect of EO on Piglet Immunity
Compared to the control group, the plasma cortisol (p < 0.01) and lysozyme (p < 0.05) levels were significantly decreased in the EO group ( Figure 3D,E). There was no significant difference in blood lymphocyte subsets between these two groups ( Figure 3F-H).

Effect of EO on Piglet Hematology, Plasma Biochemical Index, and Antioxidant Capacity
There was no significant difference in the hematology index between the two groups (p > 0.05, Supplementary Table S3). EO supplementation significantly decreased the plasma cholesterol and HDL-C level (p < 0.05) compared to the control group ( Figure  2B,D). The plasma LDL-C in the EO group was lower than in the control group (p < 0.1, Figure 2C). Additionally, there was no significant difference in plasma MDA level, SOD activity, GSH-Px activity, and T-AOC between the two groups (p > 0.05; Figure 2E-H).

Effect of EO on Piglet Immunity
Compared to the control group, the plasma cortisol (p < 0.01) and lysozyme (p < 0.05) levels were significantly decreased in the EO group ( Figure 3D,E). There was no significant difference in blood lymphocyte subsets between these two groups ( Figure 3F-H).

Effect of EO on Piglet Intestinal Epithelial Morphological Structure
The ileal villus height and villus height/crypt depth ratio in the EO group were significantly higher than the control group (p < 0.05; Figure 4A-D). Moreover, there was no significant difference in piglet jejunum (p > 0.05; Figure 4A-D). The number of goblet cells in the jejunal villus was significantly higher in the EO group than in the control  Figure 4E). Furthermore, there was no significant difference in piglet ileum (p > 0.05; Figure 4E,F).

Effect of EO on Piglet Intestinal Epithelial Morphological Structure
The ileal villus height and villus height/crypt depth ratio in the EO group were significantly higher than the control group (p < 0.05; Figure 4A-D). Moreover, there was no significant difference in piglet jejunum (p > 0.05; Figure 4A-D). The number of goblet cells in the jejunal villus was significantly higher in the EO group than in the control group (p < 0.05; Figure 4E). Furthermore, there was no significant difference in piglet ileum (p > 0.05; Figure 4E,F).

Effect of EO on Piglet Intestinal Barrier Function
The relative mRNA expression of ZO-1 was significantly higher in jejunal mucosa in the EO group than in the control group (p < 0.05; Figure 5D). And there was no significant difference in MUC1, MUC2, MUC4, claudin-1, or occludin-1 mRNA expression (p > 0.05, Figure 5).

Effect of EO on Piglet Intestinal Barrier Function
The relative mRNA expression of ZO-1 was significantly higher in jejunal mucosa in the EO group than in the control group (p < 0.05; Figure 5D). And there was no significant difference in MUC1, MUC2, MUC4, claudin-1, or occludin-1 mRNA expression (p > 0.05, Figure 5).

Effect of EO on Piglet Ileum Immune Function
Relative protein expression of genes involved in the NF-κB pathway was determined in piglet ileal mucosa ( Figure 6). EO supplement significantly decreased TLR4 and NFκB p-p65/p65 protein expression (p < 0.05).

Effect of EO on Piglet Ileum Immune Function
Relative protein expression of genes involved in the NF-κB pathway was determ in piglet ileal mucosa ( Figure 6). EO supplement significantly decreased TLR4 and p-p65/p65 protein expression (p < 0.05).

Effect of EO on Piglet Ileum Immune Function
Relative protein expression of genes involved in the NF-κB pathway was determined in piglet ileal mucosa ( Figure 6). EO supplement significantly decreased TLR4 and NFκB p-p65/p65 protein expression (p < 0.05).

Effect of EO on VFA Concentrations and Microbiome in Piglet Colonic Content
In total, 12 colonic content samples were used for VFA concentration determination and 16S rDNA sequencing. The difference between the two groups in VFA concentrations in piglet colonic content was insignificant (p > 0.05; Figure 7). Microbial α-diversity and β-diversity analysis revealed few differences between the two groups ( Figure 8A-C). The unweighted pair-group method with arithmetic mean (UPGMA) analysis disclosed similar microbial composition between the two groups ( Figure 8D). The core floras were composed of Firmicutes, Bacteroidetes, and Proteobacteria. LEfSe analysis revealed that the Erysipelotrichaceae family, Phascolarctobacterium, Holdemanella, and Vibrio genus were the biomarker bacteria in the EO group ( Figure 8E).

Effect of EO on VFA Concentrations and Microbiome in Piglet Colonic Content
In total, 12 colonic content samples were used for VFA concentration determination and 16S rDNA sequencing. The difference between the two groups in VFA concentrations in piglet colonic content was insignificant (p > 0.05; Figure 7). Microbial α-diversity and βdiversity analysis revealed few differences between the two groups ( Figure 8A-C). The unweighted pair-group method with arithmetic mean (UPGMA) analysis disclosed similar microbial composition between the two groups ( Figure 8D). The core floras were composed of Firmicutes, Bacteroidetes, and Proteobacteria. LEfSe analysis revealed that the Erysipelotrichaceae family, Phascolarctobacterium, Holdemanella, and Vibrio genus were the biomarker bacteria in the EO group ( Figure 8E).

Discussion
Weaning stress contributes to inflammation and oxidative stress, leading to an i paired intestinal barrier [10,37]. EOs are effective additives to alleviate weaning stress early-weaned piglets without resistance and environmental pollution [12]. The use o single EO has drawn the attention of researchers; however, the combined use of differe EOs needs further exploration. In the present study, we assessed the effect of an EO ble on growth performance, plasma biochemical index, immunity, intestinal epithelial m phology, barrier function, and microbiota in early-weaned piglets.
Early weaning leads to descending feed intake and decreased growth rate, owing

Discussion
Weaning stress contributes to inflammation and oxidative stress, leading to an impaired intestinal barrier [10,37]. EOs are effective additives to alleviate weaning stress in early-weaned piglets without resistance and environmental pollution [12]. The use of a single EO has drawn the attention of researchers; however, the combined use of different EOs needs further exploration. In the present study, we assessed the effect of an EO blend on growth performance, plasma biochemical index, immunity, intestinal epithelial morphology, barrier function, and microbiota in early-weaned piglets.
Early weaning leads to descending feed intake and decreased growth rate, owing to poor palatability and digestibility of the solid dry diet [38]. In this study, EO supplementation significantly improved ADG and ADFI with few changes in F/G. It agreed with the previous study that combining thymol and cinnamaldehyde led to a significant increase in ADG and ADFI in weaning piglets [39]. A possible explanation for the increased ADFI might be related to the pleasant odor and flavor of EOs, which contributed to improved appetite [40].
The separation from sows and the abrupt change in diet might be a stimulus for declined villus height and increased crypt depth in weaning piglets [41,42]. Declined villus height is mainly induced by aggravated apoptosis and compromised renewal of intestinal epithelial cells under stresses or diseases [43]. In this study, EO supplementation led to increased villus height and villus height/crypt depth ratio. In line with this, carvacrol and thymol diets enhanced the villus height/crypt depth ratio in the distal small intestine of weaned piglets [44]. However, the EO blend (a combination of thymol and cinnamaldehyde) significantly increased the villus height/crypt depth ratio in the weaned piglet jejunum instead of the ileum [22]. Different EO types and concentrations might cause different effects. Goblet cells synthesize and secret complex mucins to protect the intestinal epithelium from pathogens and toxins [45]. A tight junction is indispensable for the integrity of intestinal epithelial barrier, including occludin, zonula occludens (ZO), and claudins [46]. In this study, EO supplementation significantly augmented jejunal goblet cells in the villus, and jejunal ZO-1 mRNA expression, which agreed with the previous study that carvacrol administration significantly increased ZO-1 mRNA expression and goblet cells in the broiler small intestine [47]. Collectively, these data suggested that the EO diet contributed to ileal epithelial development and jejunal epithelial integrity.
Carvacrol and thymol possess an inhibitory effect on 3-hydroxy-3-methylglutaryl coenzyme A reductase, the rate-limiting enzyme of cholesterol synthesis [48]. Reducing plasma cholesterol and HDL-C levels might be related to the hypocholesterolemic effect of carvacrol and thymol. Elevated cortisol is a stress biomarker, and the cortisol level is increased in weaned piglets suffering from weaning stress [49]. Lysozyme is secreted by monocytemacrophage and epithelioid cells [50]. Reportedly, an augmented serum lysozyme level was found in patients with Crohn's disease and ulcerative colitis [51]. Our results showed that EO administration decreased plasma cortisol and lysozyme levels, suggesting EO relieved weaning stress in piglets. The intestine is the principal organ involved in immunity, in which the ileum plays an indispensable role. Reportedly, weaning stress has led to a severe inflammatory immune response in the piglet intestine [52], contributing to disturbed intestinal function and retarded growth performance [53,54]. The anti-inflammatory effect has been found in various EO. Cinnamic aldehyde exerted anti-inflammatory effects by targeting TLR2, TLR4, and NFκB; oregano extracts (containing sabinene hydrate, thymol, and carvacrol) relieve inflammation by decreasing pro-inflammatory cytokines and increasing anti-inflammatory cytokine synthesis [55]; cinnamaldehyde relieves inflammation by suppressing NO release, decreasing COX-2 expression, and increasing cAMP production [56]. In this study, EO supplementation inhibited TLR4 and NFκB p-p65/p65 protein expression in ileal mucosa. Collectively, EO supplementation relieved piglet stress and suppressed the ileal inflammatory TLR4/NFκB pathway.
The intestinal microbiota closely interacts with intestinal immunity [57]. In this study, little change was observed in colon microbial α-diversity and β-diversity, consistent with the previous study [24]. Bacteroidetes, Firmicutes, Proteobacteria, and Actinobacteria are the predominant phyla in most mammals [58], which agreed with our study that these phyla were the most abundant in piglet colonic digesta. The relative abundance of Erysipelotrichaceae and Phascolarctobacterium is negatively related to inflammatory disease. Reportedly, the relative abundance of Erysipelotrichaceae in piglet cecum contents of digesta was negatively correlated with the expression of inflammatory factors [59]. Phasco-larctobacterium was positively correlated with serum antioxidant capacity, and negatively correlated with serum pro-inflammatory cytokines in piglets [60]. However, the effect of Holdemanella is controversial. A previous study reported that the abundance of Holdemanella in piglet cecum content was positively related to serum pro-inflammatory cytokines [61], whereas others reported that Holdemanella showed anti-inflammatory activity in colitis patients [62]. Vibrio is a common pathogen that contributes to diseases such as intestinal inflammation and diarrhea [63]. Our result showed that Erysipelotrichaceae and Phascolarctobacterium were significantly enriched in the EO group, which might be related to the down-regulated inflammatory pathway and mitigated stress in piglets. Few studies focus on the exact function of EOs on these bacteria, and the underlying mechanism needs further exploration. Collectively, the microbiota changes induced by EO supplementation might be related to inhibited inflammatory pathways in piglets.
In conclusion, this study reported changes in growth performance, immunity, intestinal epithelial development, and intestinal flora in early-weaned piglets, caused by EO supplementation. Our results demonstrated that EO supplementation contributed to improved ADG and ADFI in the day 0 to 28 period, less stress, improved ileal epithelial development, and suppression of the ileal inflammatory TLR4/NFκB pathway. Additionally, EOs led to few changes in microbiota composition, and the enriched flora in the EO group might be related to depressed inflammatory pathways in piglets. This study provides a theoretical basis for the combined use of EO. However, further studies are needed to explain the underlying mechanism.