Ameliorative Effect of Citrus junos Tanaka Waste (By-Product) Water Extract on Particulate Matter 10-Induced Lung Damage

Citrus junos Tanaka (CJ)-related products are well-accepted by consumers worldwide; thus, they generate huge amounts of waste (peel, pulp, and seed) through CJ processing. Although some CJ by-products (CJBs) are recycled, their use is limited owing to the limited understanding of their nutritional and economic value. The exposure to particulate matter (PM) increases the risk of respiratory diseases. In this study, we investigated the ameliorative effects of CJB extracts (100, 200 mg/kg/day, 7 days) on PM10-induced (10 mg/kg, intranasal, 6 h) lung damage in BALB/c mice. Cell type-specific signaling pathways are examined using the A549 (PM10, 200 μg/mL, 6 h) and RAW264.7 (LPS, 100 ng/mL, 6 h) cell lines. The CJB extracts significantly attenuated PM10-induced pulmonary damage and inflammatory cell infiltration in a mouse model. The essential protein markers in inflammatory signaling pathways, such as AKT, ERK, JNK, and NF-κB for PM10-induced phosphorylation, were dramatically reduced by CJB extract treatment in both the mouse and cell models. Furthermore, the CJB extracts reduced the production of reactive oxygen species and nitric oxide in a dose-dependent manner in the cells. Comprehensively, the CJB extracts were effective in reducing PM10-induced lung injuries by suppressing pulmonary inflammation, potentially due to their anti-inflammatory and antioxidant properties.


Introduction
Citrus junos Tanaka (CJ), also known as yuzu, is a yellow citrus fruit widely grown in Korea, Japan, and China [1]. CJ is well-accepted by consumers because of its pleasant flavors and abundant phytochemicals. CJ has been suggested as a good source of dietary antioxidants, and its role in the prevention and treatment of various diseases has been widely studied [2][3][4]. The major phenolic compounds found in CJ are flavonoids (including naringin, hesperidin, narirutin, and neohesperidin), which are considered one of the most important sources of bioactive compounds [5][6][7]. CJ has been widely used as a beverage, ice cream, dessert, and cooking material because of its tart flavor. However, except for the edible flesh used in the processing industry, the other inedible parts, such as the peels, seeds, and pulp matrices, are mostly wasted as by-products. Although some CJ by-products (CJBs) from the food processing industry are recycled, a large quantity is still released into the environment, causing pollution issues [8][9][10]. Additionally, the use of by-products is limited owing to the lack of understanding of their nutritional and economic value.

Materials and Reagents
The CJBs used in this study were obtained from the Goheung Agricultural Cooperative Federation (Goheung, Korea) and extracted through the methods used by Lee et al. [20], with some modifications. The material was ground using a blender and extracted (70 • C) with 10 volumes of distilled water for two hours. The extract was filtered through a filter paper (Whatman, Maidstone, UK), spray-dried, and stored in a −80 • C deep-freezer. The naringin (≥95%), hesperidin (≥97%), and PM10 (ERM-CZ120) used for the certified reference material were purchased from Sigma-Aldrich (St. Louis, MO, USA).

Antioxidant Capacity and Component Assay 2.2.1. ABTS Radical Scavenging Assay
The ABTS radical scavenging activity was estimated using the method of Shalaby and Shanab [21], with some modifications. Briefly, an ABTS solution (190 µL; 1 mM AAPH and 2.5 mM ABTS in PBS; Sigma-Aldrich) was mixed with 10 µL of a CJB (1 mg/mL; distilled water (DW)) solution and allowed to react for 10 min at room temperature in the dark. The absorbance at 734 nm was measured using a Perkin Elmer Lambda 35 UV/VIS spectrometer (Perkin Elmer Inc., Waltham, MA, USA). The radical scavenging activity of the CJB extract was expressed as the ascorbic acid equivalent value (VCE).

DPPH Radical Scavenging Assay
The DPPH radical scavenging activity was evaluated through the method used by Shalaby and Shanab [21], with slight modifications. Briefly, a DPPH (Sigma-Aldrich) solution (190 µL; 50 µM in 95% EtOH; Sigma-Aldrich) was added to 10 µL of CJB (1 mg/mL; 95% EtOH) solution and incubated at room temperature for 30 min in the dark. The mixture was stirred vigorously and was allowed to stand at room temperature in the dark for 30 min. The rate of absorbance at 517 nm was monitored using a spectrophotometer (Perkin Elmer), and the results are presented as the mg VCE/g sample.

Total Polyphenol Content
The total polyphenol content (TPC) in the samples was determined using the method described by Singh et al. [22]. Briefly, 50 µL of the CJB solution (1 mg/mL; DW) was reacted with 100 µL of Na 2 CO 3 (2% in DW; Sigma-Aldrich) solution and 50 µL of the Folin-Ciocalteu solution (0.2 N; Sigma-Aldrich), blended, and preserved for 10 min. The absorbance was measured at 720 nm using a spectrophotometer (Perkin Elmer), and the results are presented as the gallic acid equivalence value (GAE).

Total Flavonoid Content
The total flavonoid content (TFC) in the samples was determined using the method of Singh et al., with slight modifications [22]. Briefly, the 500 µL CJB solution (1 mg/mL) was mixed with 200 µL NaNO 2 (10% in DW; Sigma-Aldrich), and the mixture was incubated for 6 min at room temperature. After that, 300 µL of AlCl 3 (10% in DW; Sigma-Aldrich) was added to the mixture and incubated for 6 min again, and then 2 mL of 1 M NaOH was added, blended, and preserved for 15 min at room temperature. The absorbance was measured at 510 nm using a spectrophotometer (Perkin Elmer), and the results are presented as the rutin equivalence value (RE).

Analysis of Naringin and Hesperidin Content
The naringin and hesperidin contents in the samples were determined using the method of Lee et al., with some modifications [20]. The Shiseido Nanospace SI-2 (Shiseido; Tokyo, Japan) was equipped with a quaternary pump, an online degasser, an auto platesampler, and a UV/Vis detector with a CapcellPAK C18 UG120 column (4.6 × 250 mm, 5 µm particle size), which was used for the separation. Solvent A consisted of 0.1% formic acid in water, and solvent B consisted of 0.1% formic acid in acetonitrile. The solvent gradient was listed as follows: 15% B at 0-15 min; 15-35% B at 15-35 min; 35-90% B at 35-40 min. The composition was held at 95% B for 5 min and then returned to its initial conditions, and it was maintained for 5 min to equilibrate the column. The detection wavelength was set at 280 nm. The column temperature was maintained at 40 • C in a thermostatically controlled column compartment. The flow rate was 1.0 mL/min, and the injection volume was 10 µL.

Cell Culture
RAW264.7 murine macrophage and A549 human alveolar epithelial cells were obtained from the Korean Cell Line Bank (Seoul, Korea) and maintained at 37 • C in a CO 2 incubator (Thermo Fisher Scientific, Waltham, MA, USA). The RAW264.7 and A549 cell lines were maintained in DMEM (Thermo Fisher Scientific) and RPMI (Thermo Fisher Scientific) media supplemented with 10% (v/v) fetal bovine serum (Thermo Fisher Scientific) and 1% (v/v) penicillin/streptomycin solution (Sigma-Aldrich), respectively [23].

Cell Viability Assay
The cytotoxicity of the CJBs was examined using the WST-8 Cell Viability Kit (Biomax, Seoul, Korea) according to the method of Keum et al. [24], with some modifications. In brief, the RAW264.7 and A549 cells (1 × 10 4 cells/well) were seeded in 96-well microplates and incubated for 24 h. The cells were then incubated with various concentrations of CJB extract for 24 h (i.e., 0.1, 0.2, 0.5, 1, and 2 mg/mL). Subsequently, the live cells were stained for 2 h with WST-8 dye, and the optical density was read on a microplate reader (Bio-Rad 680; Bio-Rad, Hercules, CA, USA) at 450 nm for the absorbance and at 650 nm for the subtracted background absorbance. The viability was expressed as a percentage of the control.
2.6. Intracellular Anti-Oxidative Assay 2.6.1. Nitric Oxide (NO) Analysis The levels of NO were determined by the method of Park et al. [25], with some modifications. The RAW264.7 cells (2 × 10 5 cells/well) were plated on a 24-well plate for 24 h, and then 100 ng/mL of lipopolysaccharide (LPS) and CJB solution (i.e., 0.5, 1, and 2 mg/mL) was co-treated for 6 h. The concentration of NO was measured using the Griess reagent. Equal volumes of the supernatant and Griess reagent were mixed (i.e., 50 µL each) and incubated for 15 min at room temperature. The nitrite concentration (µM) was determined by measuring the absorbance at 540 nm using a microplate reader (Bio-Rad) and was compared to a standard curve obtained using sodium nitrite.

Intracellular Levels of ROS
The intracellular levels of ROS were determined by the method of Li et al. [23]. The A549 cells (1 × 10 4 cells/well) were seeded in a 96-well plate for 24 h, and then 200 µg/mL of PM10 and CJB solution (0.5, 1, and 2 mg/mL) was co-treated for 60 min. A fluorescent probe (2 ,7 -Dichlorofluorescin diacetate, 10 µM, Sigma-Aldrich) was then loaded onto the cells and was incubated for 30 min. After the cells were washed twice with ice-cold PBS, they were immediately placed in a spectrofluorometer (Perkin Elmer) to determine the fluorescence intensity of the ROS generation at excitation and emission wavelengths of 535 nm and 485 nm, respectively.

Cell Treatment for Protein Expression
The A549 and RAW264.7 cells (1 × 10 6 cells/well) were plated in a 60 mm dish for 24 h, and then 200 µg/mL of PM10 or 100 ng/mL of LPS and CJB samples (0.5, 1, and 2 mg/mL) was co-treated for 6 h. After that, the cells were prepared for protein extraction to determine the undergoing mechanism [23].

Histological Analysis
The left lobe of the lung tissue was isolated from the mice and immediately fixed in 4% paraformaldehyde before being serially processed and embedded in paraffin. Subsequently, 3 µm tissue sections were prepared using a microtome, and the deparaffinized tissue sections were stained with hematoxylin and eosin (H&E; Sigma-Aldrich). The stained images were visualized under an inverted microscope (Olympus BH 2, Tokyo, Japan; magnification, ×100). The lung injury score was measured on a subjective scale from 1 to 3 as follows: 1, no inflammation was observed; 2, there was occasional cuffing with the inflammatory cells; and 3, most bronchi or vessels were surrounded by inflammatory cells [26].

ELISA Assay
The levels of TNF-α, IL-1β, and IL-6 in BALF were measured using specific ELISA kits (R&D Systems, Minneapolis, MN, USA) according to the manufacturer's instructions.

Immunoblot Analysis
After exposure to various treatments, the lung tissue and cell extracts were prepared and rinsed in a modified radioimmunoprecipitation assay lysis buffer on ice using the method of Park et al. [25], with slight modifications. After 20 min of centrifugation at 12,000× g at 4 • C, the supernatants were collected, and the concentrations were measured using a bicinchoninic acid protein assay kit (Sigma-Aldrich). Subsequently, equal amounts of each protein sample (20 µg) were separated by sodium dodecyl salt-polyacrylamide gel electrophoresis and transferred onto polyvinylidene difluoride membranes (Millipore, Boston, MA, USA) using a Bio-Rad semi-transfer system. After blocking (5% skim milk at room temperature for one hour), the membranes were incubated overnight with primary antibodies (β-actin, pAKT, AKT, pERK, ERK, pJNK, JNK, pNFκB, NFκB, iNOS, COX-2, TNF-α, IL-1β, and IL-6) (Cell Signaling, Danvers, MA, USA; Santa Cruz Biotechnology, Dallas, TX, USA; Abcam, Cambridge, UK)) at 1/1000 or 1/2000 dilutions with agitation at 4 • C. The membranes were then washed and exposed to horseradish peroxidase-conjugated secondary antibodies for one hour at room temperature. Finally, the proteins were visualized using enhanced chemiluminescence reagents (Bio-Rad) and examined with an Image Quant LAS-4000 chemiluminometer (GE Healthcare, Chicago, IL, USA).

Statistical Analysis
The experimental data were processed using IBM SPSS software (version 26.0; IBM Corp, Armonk, NY, USA). The data are expressed as the mean ± standard error from three independent experiments, unless otherwise specified. The differences between the groups were analyzed with one-way analysis of variance followed by Dunnett's multirange test, where #, * p < 0.05, ##, ** p < 0.01, and ###, *** p < 0.001 were considered to indicate a significant difference.

Antioxidant Capacity and Phytochemicals in CJBs
ABTS and DPPH assays are widely used for antioxidant activity determination and are based on a decrease in spectrophotometer absorption at 734 nm and 517 nm due to the reduction of the radicals scavenged by the antioxidant compounds. As shown in Table 1, the CJBs showed free radical scavenging activities with 23.47 ± 0.34 (ABTS) and 28.98 ± 0.42 (DPPH) mg VCE/g of the samples, respectively. The TPC and TFC of the CJBs were analyzed using the Folin-Ciocalteu reagent and the aluminum chloride colorimetric technique, respectively, as shown in Table 1. The TPC values of the extracts ranged from 36.68 ± 0.30 mg GAE/g of the sample. Like the TPC results, the CJBs had a TFC of 21.11 ± 0.49 mg of RE/g of the sample (Table 1). Furthermore, the most abundant bioactive compounds in citrus fruits were analyzed (i.e., naringin and hesperidin) [7] in our study ( Figure 1). The quantification data of the naringin and hesperidin content in the CJBs ranged from 4.00 ± 0.09 mg and 9.00 ± 0.03 mg/g of the sample, respectively (Table 1).

Effects of CJB on PM10-Induced Pulmonary Damage
To examine the protective effects of CJBs against PM10-induced lung injuries, changes in lung histopathology were investigated by H&E staining. As shown in Figure 2a wall. The lung injury score was calculated following the treatment with naringin and different doses of CJBs (29% reduction at 100 mg/kg and 35% reduction at 200 mg/kg) or NAR (36% reduction at 100 mg/kg), and compared with that of the PM10-treated group, revealing that the naringin and CJBs mitigated inflammatory cell infiltration and protected the lungs from PM10-induced lung damage.

Effects of CJB on PM10-Induced Pulmonary Damage
To examine the protective effects of CJBs against PM10-induced lung injuries, changes in lung histopathology were investigated by H&E staining. As shown in Figure  2a, the PM10 group exhibited inflammatory cell infiltration, with cells deposited on the alveolar wall. The lung injury score was calculated following the treatment with naringin and different doses of CJBs (29% reduction at 100 mg/kg and 35% reduction at 200 mg/kg) or NAR (36% reduction at 100 mg/kg), and compared with that of the PM10-treated group, revealing that the naringin and CJBs mitigated inflammatory cell infiltration and protected the lungs from PM10-induced lung damage.

Effects of CJBs on PM10-Induced Pulmonary Inflammation
To understand the preventative mechanisms of CJBs at the molecular level, representative pro-inflammatory cytokines, TNF-α, IL-1β, and IL-6, were analyzed first. In agreement with our histological observations, the secretion of inflammatory cytokines

Effects of CJBs on PM10-Induced Signaling Pathways
Particulate pollutants trigger and exacerbate inflammation. In this study, we investigated the regulatory effects of CJBs on PM10-induced pulmonary damage using western blotting. We examined the key proteins involved in major inflammatory signaling

Effects of CJBs on Nitric Oxide Production and Inflammation in RAW264.7 Macrophages
Alveolar macrophages engulf inhaled fine particulates, which can induce pulmonary oxidative stress. To investigate the anti-inflammatory effect of CJBs, the cytotoxicity of

Effects of CJBs on PM10-Induced ROS Generation in A549 Cells
The regulatory effects of the CJBs on PM10-induced intracellular ROS production were measured using a cell-permeable fluorescent probe. Firstly, no significant cytotoxicity of the CJBs was observed (Figure 8a). PM10 exposure dramatically elevated (by approximately 14 times) the intracellular levels of ROS compared to those in the CON group, while the CJB extract significantly reduced (32-45%) ROS generation in a dose-dependent manner compared to the PM10 group, especially at concentrations of 1 and 2 mg/mL (Figure 8b).

Effects of CJBs on PM10-Induced ROS Generation in A549 Cells
The regulatory effects of the CJBs on PM10-induced intracellular ROS production were measured using a cell-permeable fluorescent probe. Firstly, no significant cytotoxicity of the CJBs was observed (Figure 8a). PM10 exposure dramatically elevated (by approximately 14 times) the intracellular levels of ROS compared to those in the CON group, while the CJB extract significantly reduced (32-45%) ROS generation in a dosedependent manner compared to the PM10 group, especially at concentrations of 1 and 2 mg/mL (Figure 8b).

Effects of CJBs on PM10-Induced ROS Generation in A549 Cells
The regulatory effects of the CJBs on PM10-induced intracellular RO were measured using a cell-permeable fluorescent probe. Firstly, n cytotoxicity of the CJBs was observed (Figure 8a). PM10 exposure dramatic (by approximately 14 times) the intracellular levels of ROS compared to thos group, while the CJB extract significantly reduced (32-45%) ROS generatio dependent manner compared to the PM10 group, especially at concentratio mg/mL (Figure 8b).

Discussion
Citrus fruit waste, a cost-effective source of bioactive compounds, has pharmacological importance; however, it has not attained commercial importance and is largely disposed of as municipal waste, creating a big challenge for processing industries as well as for pollution-monitoring agencies. Here, we assessed the ability of CJBs, or citrus fruit waste, to inhibit PM10-derived pulmonary damage using in vitro and in vivo approaches. Collectively, the CJBs were effective in reducing PM10-induced lung damage by suppressing pulmonary inflammation, potentially due to their anti-inflammatory and antioxidant properties.
The established health benefits of phytochemicals, which mainly act as antioxidants (e.g., polyphenols and flavonoids) due to their biological activity, necessitate their quantification in food [27][28][29]. The hydrogen-donating ability of the CJB extract was determined using the ABTS and DPPH radical scavenging methods. In addition, the total soluble phenol and flavonoid contents of the CJB extract were determined by the Folin-Ciocalteu assay and by using a method based on the formation of a flavonoid-aluminum complex, respectively. As evidenced in the data reported in Table 1, our results for the ABTS and DPPH assays showed that the CJB extract had a strong radical scavenging capacity equivalent to 23.47 ± 0.83 mg and 28.98 ± 1.03 mg of vitamin C, respectively. Additionally, the CJB extract contained 36.68 ± 0.30 mg of gallic acid, which was equivalent to the total polyphenol content, and 21.11 ± 0.49 mg of rutin, which was equivalent to the total flavonoid content.
The health benefits of citrus fruits have mainly been attributed to the presence of bioactive compounds such as flavonoids (e.g., naringin and hesperidin) [30]. For this reason, the naringin and hesperidin contents were analyzed in this study (Table 1, Figure 1). The naringin and hesperidin contents were identified in the CJB extracts with values of 4.00 ± 0.09 and 9.00 ± 0.03 mg of g sample, respectively. Generally, citrus peels have more abundant flavonoids and a higher content than pulps and juices [31], which is consistent with our results (data not shown). Here, we chose naringin, which has been used mostly in citrus-related research [32,33], as a positive control to investigate its efficacy against PM10-derived pulmonary damage.
PMs are composed of carbon cores and several soluble and insoluble components including acids, organic chemicals, metals, endotoxins, pollen, and fungal debris on their surface [34]. Therefore, the underlying mechanisms by which PM exerts its biological effects are complex. PM exposure may affect many cell types at various levels of immune regulation [35]. Inflammation is considered the central mechanism in the development of various PM-induced pulmonary diseases [36]. Several studies have suggested that endotoxins in PM10 are positively linked to TNF-α, IL-1β, and IL-6 production [37,38]. Likewise, in our experimental conditions, PM10 resulted in histological changes (Figure 2a,b) in mouse lung tissues and increased the inflammatory response in BALF (Figure 3), which is a well-known marker for lung inflammation. On the other hand, the CJB extract treatment significantly prevented PM10-induced histological damage and inflammatory responses (i.e., TNF-α, IL-1β, and IL-6) in BALF, which was enriched with non-cellular and cellular contents (e.g., cytokines and epithelial cells) from the lung alveoli. Thus, elevated levels of inflammatory cytokines in BALF are surrogate markers of lung inflammation.
Oxidative stress is a consequence of excess ROS generation, whereas antioxidant defenses are suppressed. Several studies have supported the notion that oxidative stress is an important mechanism in PM10-induced inflammatory responses, cytotoxicity, and carcinogenesis [39,40]. ROS are generated by various endotoxins (e.g., PM10 and LPS) to activate downstream signaling pathways in order to regulate the inflammatory response. Several studies have demonstrated that PM can trigger several signaling pathways to regulate PM10-induced inflammatory responses, including the AKT, ERK, JNK, and NF-κB signaling pathways [14,41]. The PI3K/AKT signaling pathway is known to play an important role in cell inflammation, apoptosis, angiogenesis, and metastasis. The ERK and JNK signaling pathways are critical intracellular signal-transduction systems and, in general, are activated by a broad array of extracellular and intracellular stimuli. In addition, the NF-κB signaling pathway is a ubiquitous modulator activated by a multitude of stimulants that regulate inflammation, cell cycles, and immune responses. In our study, PM10 stimuli induced ROS production ( Figure 8b) and activated the AKT, ERK, JNK, and NF-κB signaling pathways, leading to pulmonary inflammation. We showed that PM10 exposure augmented the phosphorylation of AKT, ERK, JNK, and NF-κB, but the naringin and CJB treatments significantly suppressed the phosphorylation activity in mice ( Figure 4). Next, the phosphorylation of AKT, ERK, JNK, and NFκB was assessed in PM10-treated A549 lung cells, which provided evidence that the CJB extract suppressed inflammatory responses via pathways involving AKT, ERK, JNK, and NF-κB ( Figure 5). Our corroborating data also showed that downstream inflammatory markers corresponded to AKT, ERK, JNK, and NF-κB activation. iNOS, COX-2, TNF-α, IL-1β, and IL-6 were effectively downregulated by the CJB extract treatment in both the mouse lung tissue and A549 lung cell lines, indicating that the CJB extract is a definite anti-inflammatory agent.
Several studies have reported that PM10 contains endotoxins that act as LPS stimuli to induce inflammation [42,43]. In addition, the activation of macrophages by endotoxins (i.e., LPS) is a major inflammatory induction mechanism [44]. Notably, NO plays a key role in inflammatory responses, and cytokine-activated macrophages trigger NO release [43]. In this study, the CJB extract treatment decreased NO production in the RAW264.7 mouse macrophages in LPS induction (Figure 3.6b). Moreover, LPS-induced ROS activated AKT, ERK, JNK, and NF-κB phosphorylation, which was highly suppressed by the CJB extract treatment. In addition, the CJB extract treatment suppressed the expression of pro-inflammatory cytokines in the LPS-treated RAW264.7 macrophages (Figure 7), which might have been due to the excessive production of ROS by LPS induction. These findings are consistent with the results obtained in mice and lung cells.
ROS scavenging in cell line models, such as cellular antioxidant assays, was performed because test tube-level assays were not sufficient to prove the biological effects. Fluorescent probe staining was used to determine the intracellular ROS levels in this study (Figure 8). We successfully induced the overproduction of ROS in the A549 cells using PM10, and the CJB extract treatment significantly suppressed ROS levels in a dose-dependent manner for PM10.

Conclusions
This study demonstrated the prophylactic effects of CJB extract on PM10-induced lung damage and proposed key inflammatory signaling pathways regulated by CJB extract treatment in various models (e.g., in vivo and in vitro). Specifically, we confirmed that CJB treatment effectively prevents PM-induced inflammatory responses by reducing the phosphorylation of AKT, ERK, JNK, and NF-κB and the secretion of cascaded pro-inflammatory cytokines. Furthermore, CJB extract reduces the production of ROS and RNS in a dosedependent manner. However, there are limitations; this was an acute study, and a high dose of PM10 (10 mg/kg) was chosen to induce the phenotype. Thus, a long-term chronic study might be needed to further confirm the dietary effects as functional ingredients.