Effects of School-Based Interventions on Reducing Sugar-Sweetened Beverage Consumption among Chinese Children and Adolescents

We set up a series of school-based interventions on the basis of an ecological model targeting sugar-sweetened beverage (SSB) reduction in Chinese elementary and middle schools and evaluated the effects. A total of 1046 students from Chinese elementary and middle schools were randomly recruited in an intervention group, as were 1156 counterparts in a control group. The interventions were conducted in the intervention schools for one year. The participants were orally instructed to answer all the questionnaires by themselves at baseline and after intervention. The difference in difference statistical approach was used to identify the effects exclusively attributable to the interventions. There were differences in grade composition and no difference in sex distribution between the intervention and control groups. After adjusting for age, sex, and group differences at baseline, a significant reduction in SSB intake was found in the intervention group post intervention, with a decrease of 35.0 mL/day (p = 0.034). Additionally, the frequency of SSB consumption decreased by 0.2 times/day (p = 0.071). The students in the elementary schools with interventions significantly reduced their SSB intake by 61.6 mL/day (p = 0.002) and their frequency of SSB consumption by 0.3 times/day (p = 0.017) after the intervention. The boys in the intervention group had an intervention effect of a 50.2 mL/day reduction in their SSB intake (p = 0.036). School-based interventions were effective in reducing SSB consumption, especially among younger ones. The boys were more responsive to the interventions than the girls. (ChiCTR, ChiCTR1900020781.)


nd Adolescent
30 May 2021A185CE24978C987E69A5BB1BD7CC1FF210.3390/nu13061862Received: 7 April 2021 Accepted: 27 May 2021sugar-sweetened beverageschool-basedinterventionecological modeldifference in difference approach
We set up a series of school-based interventions on the basis of an ecological model targeting sugar-sweetened beverage (SSB) reduction in Chinese elementary and middle schools and evaluated the effects.A total of 1046 students from Chinese elementary and middle schools were randomly recruited in an intervention group, as were 1156 counterparts in a control group.The interventions were conducted in the intervention schools for one year.The participants were orally instructed to answer all the questionnaires by themselves at baseline and after intervention.The difference in difference statistical approach was used to identify the effects exclusively attributable to the interventions.There were differences in grade composition and no difference in sex distribution between the intervention and control groups.After adjusting for age, sex, and group differences at baseline, a significant reduction in SSB intake was found in the intervention group post intervention, with a decrease of 35.0 mL/day (p = 0.034).Additionally, the frequency of SSB consumption decreased by 0.2 times/day (p = 0.071).The students in the elementary schools with interventions significantly reduced their SSB intake by 61.6 mL/day (p = 0.002) and their frequency of SSB consumption by 0.3 times/day (p = 0.01 ) after the intervention.The boys in the intervention group had an intervention effect of a 50.2 mL/day reduction in their SSB intake (p = 0.036).School-based interventions were effective in reducing SSB consumption, especially among younger ones.The boys were more responsive to the interventions than the girls.(ChiCTR, ChiCTR1900020781.

Introduction

Each year, sugar-sweetened beverage (SSB) intake is related to the loss of about 8.5 million disability-adjusted life years (DALYs), and three quarters of this burden occurs in low-and middle-income countries [1].SSBs were defined as nonalcoholic beverages sweetened by sugar, such as carbonated beverages, sugar-sweetened fruit or vegetable juice beverages, etc.The frequent consumption of excess amounts of SSBs is a risk factor for obesity [2], type 2 diabetes [3], cardiovascular disease [4], and dental caries

siology of SSB co
sumption explained the unfavorable health outcomes linked to SSBs.SSBs contain energy but no micro-and macronutrients, except glucose and fructose.Excessive glucose intake is known for causing adverse glycemic effects on metabolism [6], and fructose can be converted into fat, unfettered by the cellular controls that prevent unrestrained lipid synthesis from glucose [7].The adverse health outcomes linked to SSBs occur in both adults and children [8,9].Behavioral factors such as diet linked to mortality causes are preventable and both shape and are shaped by the social environment [10].Studies have shown that interventions can achieve favorable results in reducing SSB intake in children but not in adults [11].It is possible that interventions might have effects on individuals who were before or at their behavioral development period rather than those already forming behavioral models.

Comprehensive school-based program achieved some changes on di s [12,13].School-based interventions aiming at decreasing SSB consumption have shown promising results in previous Western studies [14].More than half of the children and adolescents in China consumed SSB, and the increasing consumption of SSBs has created the obvious threat of obesity among Chinese children and adolescents [15,16].However, there has been no study reporting evaluations of school-based interventions tackling SSB reduction in China.Considering the different environmental and cultural backgrou

s compared to Western countries, we set up a ser
es of school-based interventions on the basis of an ecological model targeting SSB reduction in Chinese elementary and middle schools and evaluated the effects on SSB reduction among Chinese children and adolescents.


Materials and Methods


Study Population

We adopted epidemiological methods to establish a parallel control g oup intervention experiment for one year, from February 2019 to January 2020, in Shanghai, China.Three elementary schools and two middle schools were randomly recruited into the intervention group

and the same number of adjacent counterparts int
the control group.Students in the 3rd and 4th grades from the selected elementary schools and the 6th and 7th grades from the selected middle schools were enrolled into the intervention or control group according to their school's allocation.The participants were not informed of which group they were assigned.

The theoretical minimum sample size was 1051 for the intervention and control groups, respectively, following the formula of sample size calculation for experiments with comparisons between two different group :
n = [(Z α/2 + Z β )S/δ] 2
We set α = 0.05 and β = 0.10.S represented standard deviation of SSB intake, andδ represented allowance error.

We decided to select 220 students in each grade of a school and a total of 1100 students in both the intervention and control groups.After enrollment, 1082 and 1259 students were in the intervention and control groups, respectively.By eliminating the students lost to follow up, there were 1046 in the interven ion group and 1156 in the contro group (Figure 1).

April 2021) with the registration number of ChiCTR1900020781 (registered 19 Jan ary 2019, http://www.chictr.org.cn/showproj.aspx?proj = 35002).


SSB Intake, Frequency, and Knowledge Assessment

The nvestigators were public health doctors in local community hea th centers and received a standard training course on the facilitation of the questionnaires.The participants were orally instructed to answer all the questionnaires by themselves.The investigators verified the answers after each questionnaire was completed.

The information collected at the baseline and post one-year intervention were both at the interval between January to February, just before and after the complete one-year intervention period.


Enrollment

All the students in the 6th and 7th grades from the selected middle schools and the 3rd and 4th grades from the selected elementary schools were enrolled at baseline.(n = 1082) All the students in the 6th and 7th grades from the selected middle schools and the 3rd and 4th grades from the selected elementary schools were enrolled at baseline.(n = 259) This study was approved by the Shanghai Municipal Center for Disease Control on 17 May 2017 (No. 2017-18).Informed consent was obtained from each participant or the participant's parents or guardian before the research.The study complied with the code of ethics of the World Medical Association (D

laration of He
sinki).This intervention study was registered at the Chinese Clinical Trial Registry (http://www.chictr.org.cn,accessed on 7 April 2021) with the registration number of ChiCTR1900020781 (http://www.chictr.org.cn/showproj.aspx?proj=35002, accessed on 19 January 2019).


SSB Intake, Frequency, and Knowledge Assessment

The investigators were public health doctors in local community health centers and received a standard training course on the facilitation of the questionnaires.The participa ts were orally in tructed to answer all the questionnaires by themselves.The investigators verified the answers after each questionnaire was completed.

The information collected at the baseline and post one-year intervention were both at the interval between January to February, just before and

ter the complete one-
ear intervention period.

A general questionnaire was used to obtain demographic information and SSB-related knowledge.There were six questions related to SSB consumption and health which were used to assess the SSB-related knowledge of the participants.The participant received one point if they gave the right answer.A full score was six.The six questions were as follows:

A.

I am not going to get fat if I consume SSBs frequently.B.

SSBs contain many nutrients.C.

SSBs are the best choice for quenching my thirst.D.

I should not have even one sip of an SSB.


E.

SSBs bring the body extra energy because the sugar in it can be easily absorbed but they hardly make one feel full.F.

SSBs have empty energy that will not pose a threat to health.

An SSB frequency questionnaire was administrated to collect information on SSB intake and frequency in the past 3 months.This questionnaire originated from a former food frequency questionnaire that was developed and validated by the China Center for Disease and Control and Prevention [17].SSBs were defined as nonalcoholic beverages sweetened by sugar, excluding fresh juice, categorized as carbonated beve

sweetened fruit or vegetable juice b
verages, protein-based beverages, probiotic beverages, milk-based beverages, bottled tea beverages, coffee drink, and typical Southeast Asian milky tea (a popular SSB in China).According to each SSB category, SSB consumption frequency was listed in terms of consumption times per day, week, month, or year, and the amount consumed each time was recorded.

The survey was conducted in the pre-and post-intervention periods using the same questionnaires.No disastrous events such as rain or snow disasters that would have

ffected the normal food supply took place during the research period.The weather was typical of t
e marine climate in Shanghai, China.


Interventions

The interventions were designed using the structures of an ecological model targeting individuals and their environment, including multiple levels of influence [18].The interventions were conducted for one year by the teachers in the intervention schools with the collaboration of public health doctors in local community health centers.The control schools had no intervening events.All the participating schools followed the city-wide education schedule during the intervention period.a.


I dividual level

Hold "restricting SSBs"-themed class meeting every semester (twice a year) and praise students with outstanding health behaviors (the themed class meeting was designed to provide SSB-related knowledge regarding the six questions about SSB-related knowledge in the questionnaire).Distribute SSB-related knowledge materials every semester (twice a year).Record one's own SSB behaviors once per week.


b. Family level

Establish WeChat (the most popular social communication application in China) groups for parents and publish new media promotional materials once a month.


c. Peer level

Distribute promotional cards to students every semester (twice a year; the cards showed cartoon figures and info

ation regarding t
e six questions on

B-related knowledge in the questionnaire, as well as mottos and goals for the new se
ester).


d. School level

Carry out a blackboard painting activity with the theme of "understanding SSBs" every semester (twice a year).

Forbid selling SSBs on campus during the intervention.


e. Community level

Negotiate with stores near the gates of schools not to sell SSBs to students.


Statistical Analyses

Statistical analyses were conducted using the SAS statistical software (v.9.4; SAS Institute, Cary, NC, USA).The comparisons were conducted between the participants in the same level of different schools different groups and pre and post intervention.T tests were applied to determine the differences between the intervention and control groups pre and post intervention.The difference in difference (DID) statistical approach was used to identify the net effects exclusively attributable to the interventions in this study [19].The DID approach was designed for quasi-experimental research studying causal relationships in public health settings where randomized controlled trials (RCTs) are infeasible or unethical.In the current analysis of DID, an interactive item of the variate representing group classifica

on and a va
iate representing pre-or post-intervention classification was included in the multivariate general linear regression models, representing the intervention effect.Age, sex, and the variates mentioned above were included in the same models as covariates.A two-sided p < 0.05 was considered to indicate statistical significance.


Results


Characteristics of the Participants

At baseline, 1082 subjects were recruited in the intervention group and 1259 subjects in the control group.At one-year follow-up, 1046 remained in the intervention group and 1156 in the control group.The retention rates were 96.7% and 91.8% in the intervention and control groups, respectively.Differences in sex distribution were not observed between the two groups either pre or post intervention.The differences in grade composition were significant between the groups both pre and post intervention (Table 1).At baseline, the SSB intakes were 286.0 ± 266.5 mL/day and 286.0 ± 288.2 mL/day in the intervention and control groups, respectively.The frequencies of SSB consumption were 1.6 ± 1.6 times/day and 1.7 ± 1.9 times/day, respectively.The scores for SSB-related knowledge were 4.3 ± 1.2 and 4.5 ± 1.0, respectively.There was no difference in the SSB intake and frequency of SSB consumption between the two groups and their subgroups (p > 0.05).The scores for SSB-related knowledge were significantly different between the two groups (p < 0.001).

After the one-year intervention, the SSB intakes were 220.9 ± 262.3 mL/day and 254.4 ± 268.9 mL/day in the intervention and control gro ps, respectively.The frequencies of SSB consumption were 1.1 ± 1.5 times/day and 1.4 ± 1.7 times/day, respectively.The scores for SSB-related knowledge were 4.4 ± 1.3 and 4.5 ± 1.1, respectively.Significant differences existed in the SSB intake and the frequency of SSB consumption between groups (p = 0.003, p < 0.001).The scores for SSB-related knowledge showed no statistical difference between the groups (p = 0.060) (Table 2).


The Effects on SSB Intake, Frequency, and Knowledge Attributed to the Interventions

After adjusting for age, sex, and group differences at baseline, a significant reduction in SSB intake which was exclusively attributed to the interventions was found in the intervention group post intervention, with a decrease of 35.0 mL/day (p = 0.034).Additionally, the frequency of SSB consumption decr ased by 0.2 times/day, with a borderline significance (p = 0.071), due to the intervention.No statistically significant effect was found in the score for SSB-related knowledge after the intervention (p = 0.347).

After intervention, the participants in the elementary schools with the intervention significantly reduced their SSB intake by 61.6 mL/day (p = 0.017) and their frequency of SSB consumption by 0.3 time/day (p = 0.002), but no changes were observed in the SSB intake and frequency of SSB consumption among those from middle schools with the intervention (p = 0.945 and p = 0.978, respectively).The boys in the intervention group had an intervention effect of a 50.2 mL/day reduction in SSB intake (p = 0.036), while the girls presented no significant intervention effect in SSB intake (p = 0.403) (Table 3).


Discussion

I the current study, the one-year school-based interventions achieved favorable effects in reducing SSB consumption among Chinese children and adolescents.Regarding the feasibility of the intervention implemented, each participant was not assigned randomly into an intervention or control group.Actually, the participants were allocated indiscriminately into interventions or control groups according to their school's allocation in the study.Moreover, other citywide health promotion events at the time or the underlying natural growth of individuals might have affected their SSB consumption as well.All these factors made it difficult to identify the effects exclusively attributed to the current interventions.The DID approach provided an alternative means for us to study the net effects on the SSB consumption changes which were exclusively attributable to the interventions [19,20].After adjusting for age, sex, and group differences at baseline, we discovered a substantial scale of SSB redu tion attributable to the interventions in this study.The school stood out as one of the most common settings to improve health behaviors [21].Previous studies indicated that Western school-based interventions were promising to reduce SSB consumption [14].Our results give more evidence regarding school-based interventions in the setting of an Eastern culture and environment.The current results also supported the theory that the ecological model helped to develop an environment conducive to change, facilitating the adoption of healthy behaviors [22].

We found that the beneficial effects occurred among the younger children rather than the adolescents in the current study.Both the amount and the frequency of SSB consumption reduced after the one-year intervention, while SSB-related knowledge also increased modestly among the younger children.However, there was no obvious change in the amount and the frequency of SSB consumption and SSB-relate

knowledge am
ng the participants in the middle schools between pre-and post-intervention.These findings coincided with previous studies showing that interventions focusing on SSB control are more effective on younger children than older ones [23,24].This also suggests that future interventions or policies aiming at reducing SSBs should target younger children in

der to achieve more favorable
cost-effective outcomes.

We observed that the consumption of SSBs decreased in the boy participants but not in the gir