Cameroonian Spice Extracts Modulate Molecular Mechanisms Relevant to Cardiometabolic Diseases in SW 872 Human Liposarcoma Cells

The molecular pathophysiology of cardiometabolic diseases is known to be influenced by dysfunctional ectopic adipose tissue. In addition to lifestyle improvements, these conditions may be managed by novel nutraceutical products. This study evaluatedthe effects of 11 Cameroonian medicinal spice extracts on triglyceride accumulation, glucose uptake, reactive oxygen species (ROS) production and interleukin secretion in SW 872 human adipocytes after differentiation with 100 µM oleic acid. Triglyceride content was significantly reduced by all spice extracts. Glucose uptake was significantly increased by Tetrapleura tetraptera, Aframomum melegueta and Zanthoxylum leprieurii. Moreover, Xylopia parviflora, Echinops giganteus and Dichrostachys glomerata significantly reduced the production of ROS. Concerning pro-inflammatory cytokine secretion, we observed that Tetrapleura tetraptera, Echinops giganteus, Dichrostachys glomerata and Aframomum melegueta reduced IL-6 secretion. In addition, Xylopia parviflora, Monodora myristica, Zanthoxylum leprieurii, and Xylopia aethiopica reduced IL-8 secretion, while Dichrostachys glomerata and Aframomum citratum increased it. These findings highlight some interesting properties of these Cameroonian spice extracts in the modulation of cellular parameters relevant to cardiometabolic diseases, which may be further exploited, aiming to develop novel treatment options for these conditions based on nutraceutical products.


Introduction
The use of well-characterized nutraceutical products, in addition to lifestyle changes, is an interesting option for prevention and management of chronic cardiovascular and metabolic diseases, including obesity, type 2 diabetes mellitus (T2DM), atherosclerosis and the metabolic syndrome [1], especially in milder pathological conditions [2,3]. In this context, dysfunction of the adipose tissue and derangement of adipocyte biology play a pivotal role involving several pathophysiological mechanisms including insulin resistance, oxidative stress, and low-grade chronic inflammation. Interestingly, many plant extracts used in traditional medicine were found to mitigate obesity and related dysmetabolic conditions in in-vitro experimental models as well as in clinical studies [4]. Our research group, along with other researchers, has previously investigated the composition and the molecular effects of several spice extracts from Cameroonian plants commonly used in traditional medicine. In particular, we explored the ability of these extracts to modulate the activity of some enzymes relevant to the control of cardiometabolic functions [5], the antioxidant and anti-inflammatory activities by gastric epithelial cells [6] and glucose uptake and antioxidant activity in human HepG2 cells [7].
Based on this knowledge, in this study we extended the evaluation of some potentially useful effects of these Cameroonian spice extracts in the context of adipocyte biology and pathophysiology. To address this issue, we took advantage of human SW 872 human liposarcoma cells differentiated to adipocytes by oleic acid (OA) as the model system, which previously proved suitable for testing the in-vitro metabolic activities of plant-derived extracts [8]. More specifically, the present study aimed at assessing the effects of these spice extracts on triglyceride (TG) accumulation, glucose uptake modulation, antioxidant activity and regulation of pro-inflammatory cytokine release.

Preparation of Plant Extracts
Plant material comprised 11 spices (Xylopia aethiopica (Dunal) A.Rich., Xylopia parviflora (A. Rich) Benth, Scorodophloeus zenkeri Harms, Echinops giganteus A.Rich., Monodora myristica (Gaertn.) Dunal, Tetrapleura tetraptera (Schum. & Thonn.) Taub., Dichrostachys glomerata (Forssk.) Chiov., Afrostyrax lepidophyllus Mildbr., Aframomum melegueta K.Schum., Aframomum citratum (C.Pereira) K.Schum., and Zanthoxylum leprieurii Guill. & Perr.) harvested in different sites of the region of West Cameroon. Samples included fruits, seeds and roots, as identified in the National Herbarium of Cameroon (https://irad.cm/index.php/fr/, accessed on 14 October 2021) in Yaoundé (Cameroon) compared to the deposited specimens. The preparation of the hydroethanolic extracts was conducted as previously reported [5]. Briefly, air-dried and powdered samples (100 g) were extracted using a 70% hydroethanolic mixture at room temperature and in dark conditions. The mixture was filtered, concentrated under reduced pressure, and frozen to give crude extracts. They were then lyophilized and stored at −20 • C. Spice extract stock solutions (100 mg/mL) dissolved in DMSO were prepared, aliquoted, and kept at −80 • C. The stock solution of spice extracts in DMSO was diluted in culture medium to the appropriate concentrations for cell treatments. For more details, please check Table 1 in [7]. The same concentration of DMSO (in any case never exceeding 0.1%) was also added to all control cultures.

Cell Cultures and Differentation
The SW 872 human liposarcoma (ATCC ® HTB-92 ™) cell line was from the American Type Culture Collection (ATCC ® , Manassas, VA, USA) and grown as recommended. Cells were cultured in DMEM-F12 culture medium with L-Glutamine and HEPES 25 mM supplemented with 10% fetal bovine serum (FBS), 1% penicillin (100 U/mL), and streptomycin (100 µg/mL). They were kept in culture in 100-mm diameter Petri dishes at 37 • C in a humidified atmosphere containing 5% CO 2 . Once they reached 80100% confluence, SW 872 cells were treated with 100 µM OA to initiate cellular differentiation into adipocytes [9,10].

Oil-Red-O Staining
To assess intracellular lipid accumulation (a marker of adipocyte differentiation) of SW 872 cells, we used the Oil-red-O (ORO) staining. SW 872 cells were seeded in 24-well plates, were allowed to adhere until 90-100%, confluence and then were treated with 100 µM OA for 7 days. After medium removal, cells were washed with phosphate-buffered saline (PBS) and fixed with 4% formaldehyde in PBS for 1 h (room temperature). ORO working solution (0.2% ORO in 40% isopropanol) was added to the culture dish and incubated at room temperature for 20 min. After a wash with sterile distilled water, cell images were collected with a light microscope (ZEISS, Milan, Italy).

MTS (3-(4,5-Dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2Htetrazolium) Cell Viability Assay
Cell viability assay [11] was performed using the Cell Titer 96 aqueous non-radioactive cell proliferation assay (Promega, Madison, WI, USA) following the method previously described [12] with minor modifications. Briefly, SW 872 cells were seeded in a sterile, flat-bottom 96-well plate at a density of 2 × 10 5 cells/well and incubated at 37 • C for 24 h in a humidified incubator containing 5% CO 2 . Extracts at different concentrations (1, 10, 25, 50, and 100 µg/mL) were prepared in fresh serum-free DMEM-F12 medium and 100 µL of each treatment was added to each well and then incubated for 24 and 48 h. Subsequently, 20 µL of the MTS reagent in combination with the electron coupling agent phenazine methosulfate was added to each well and allowed to react for 1 h at 37 • C. After 2 min of shaking at lowest intensity, the absorbance at 490 nm was measured (EnSpire PerkinElmer Multimode Plate Reader). In the same conditions, controls and blanks, consisting of % cell viability = mean sample absorbance − mean blank absorbance mean control absorbance − mean blank absorbance × 100 (1) Three separate experiments run in triplicate replicates were conducted.

Morphological Analysis
Cells were cultured in sterile, flat-bottom 6-cm 2 dishes for 24 h. Different concentrations of extracts (1, 10, 20 and 100 µg/mL) were prepared in serum-free media and 2 mL of each treatment was added to each dish and incubated for 24 h and 48 h. After treatment, cells were visualized with a light microscope (ZEISS, Milan, Italy) using 10×, 20× and 32× magnifications.

Interleukin-6 and Interleukin-8 Measurement
Interleukin-6 (IL-6) and interleukin-8 (IL-8) medium content was quantified using human IL-6 and IL-8 ELISA kits, as previously described [13]. Briefly, Corning 96-well EIA/RIA plates from Merck Life Science (Milan, Italy) were coated with the antibody provided in the ELISA Kit (Peprotech, London, UK) overnight at 4 • C. After blocking the reaction, 200 µL of samples in duplicate were transferred into wells and incubated at room temperature for 1 h. The amount of IL-6 and IL-8 in the samples was detected by the EnSpire Plate Reader (signal read: 450 nm, 0.1 s) using biotinylated and streptavidin-HRP conjugate antibodies, evaluating the 3,3 ,5,5 tetramethylbenzidine (TMB) substrate reaction. Quantification of IL-6 and IL-8 was done using an optimized standard curve provided with the ELISA Kit (8.0-1000.0 pg/mL). Resveratrol (10 µM) and EGCG (40 µM) were used as positive control for IL-6 and IL-8, respectively.

Triglyceride Content Measurement
To determine the accumulation of TG in the cells we used a sensitive assay using a TG quantification reagent (Vaktro Scientific, Patras, Greece). In this assay, TG are converted to free fatty acids and glycerol. Then, glycerol is oxidized to generate a colorimetric (570 nm)/fluorometric (λex = 535 nm/λem = 587 nm) product. The kit can detect 2 pmole-10 nmole (2-10,000 µM range) of TG. All samples and standards were measured in duplicate. The data obtained were interpolated in an appropriate triglycerides standard curve after background correction.

Measurement of ROS Production
The ntracellular ROS level in SW 872 cells were determined by a fluorometric assay, applying the oxidant-sensitive fluorescence probe CM-H 2 DCFDA according to the method described by Piazza et al. [14]. Cells were seeded in 96-well black plates at a density of 2 × 10 5 cells/well, and after reaching 90% confluence, differentiated as previously described for 7 days with 100 µM oleic acid. Further, they were incubated with plant extracts at different concentrations ranging from 0 to 20 µg/mL for 24 h. Cells were then treated with 20 µM CM-H 2 DCFDA and then incubated for 1 h with 500 µM hydrogen peroxide to induce ROS production. The resulting fluorescence intensities were quantified at an excitation wavelength of 485 nm and an emission wavelength of 535 nm using the EnSpire Plate Reader (Perkin Elmer, Milan, Italy). Eagle w/Earle's BSS without glucose. Finally, the 2-NBDG uptake was measured detecting the emitted fluorescence with FACS (BD LSRFortessa™ Flow Cytometer, Milan, Italy).

Western Blotting Analysis
Total protein extracts from SW 872 cells were obtained by lysing cells in 150 µL RIPA buffer containing a mix of protease and phosphatase inhibitors (Roche Diagnostics, Monza, Italy). Forty µg of proteins and a molecular mass marker (Novex ® Sharp Protein Standard, InvitrogenTM; Life Technologies, Monza, Italy) were separated on 10% sodium dodecylsulfate-polyacrylamide gel (SDS-PAGE) under denaturing and reducing conditions. They were then transferred to a nitrocellulose membrane by using the iBlotTM Gel Transfer Device (InvitrogenTM; Life Technologies). The membranes were washed with Tris-Buffered Saline-Tween 20 (TBS-T) and non-specific binding sites were blocked in TBS-T containing 5% BSA (Merck Life Science, Milan, Italy) or non-fat dried milk at room temperature for 60 min. The blots were incubated overnight at 4 • C with anti-pAKT(Ser473), (1:150; Millipore, Merck Life Science), anti-AKT (1:1000; Cell Signaling Technology, Milan, Italy), and anti β -Actin (Millipore, diluted 1:5000) (5% BSA or non-fat dried milk). Membranes were washed with TBS-T and then exposed for 30 min at room temperature to a diluted solution (5% non-fat dried milk) of the secondary antibodies. Immunoreactive bands were detected by exposure of the membranes to ClarityTM Western ECL chemiluminescent substrates (Bio-Rad Laboratories, Segrate, Italy) for 5 min and images were acquired with a ChemiDocTM XRS System (Bio-Rad Laboratories, Milan, Italy). Densitometric readings were assessed using the ImageLab software(6.1 version, Bio-Rad Laboratories, Milan, Italy).

Statistical Analysis
Results from at least three independent experiments carried out in triplicate were expressed as mean ±SD, values or as a mean percentage (%) compared to a control. Graphs and data were analyzed using GraphPad Prism version 8. The statistical analysis was made with one-way ANOVA followed by Tukey test, considering statistically significant p < 0.05.

Spice Extracts up to 25 µg/mL Do Not Affect SW 872 Cell Viability and Morphology
Preliminary experiments were conducted to assess the effect of 11 Cameroonian spice extracts on the viability of differentiated SW 872 cells by MTS assay and morphological analysis. The extraction protocol, molecular composition, and potential cytotoxic effects in other cell lines of the spice extracts used in this study have been previously reported [5][6][7]. The MTS assay was conducted after treatment with different concentrations (1-100 µg/mL) of extracts for 24 h and 48 h. Viability threshold was set at 80% [15]. All spice extracts incubated for 24 h or 48 h proved non toxic up to the concentration of 25 µg/mL ( Figure S1). More specifically, after 24 h of incubation, 6/11 extracts proved toxic at 50 µg/mL and after 48 h 2/11 plants were toxic already at 25 µg/mL. Toxicity at 100 µg/mL was observed for 10/11 extracts in SW 872 cells, independently of the incubation time ( Figure S1). We also investigated whether spice extracts affected cell morphology in addition to viability. The morphology of differentiated SW 872 cells was not affected by treatment for 24 h and 48 h with 1-25 µg/mL spice extracts. At 100 µg/mL, the maximum concentration tested, all but one (A. lepidophyllus) spice extracts produced morphological changes, including cell death, rounding, and shrinking (representative images obtained with X. aethiopica and A. lepidophyllus extracts are shown in Figure S2). Based upon these results obtained by MTS assay and morphological analysis, all treatments with spice extracts were conducted for 24 h or 48 h at the concentration of 20 µg/mL or lower.

All Spice Extracts Reduce Triglyceride Accumulation in Differentiated Adipocytes
SW 872 cells were differentiated to adipocytes with 100 µM OA for seven days, according to a previously published protocol [8,9]. This treatment led to a marked intracellular lipids accumulation, as observed by ORO staining analysis ( Figure 1) and quantification of TG content (Table 1), indicating the acquisition of a mature adipocyte phenotype. Of interest, differentiation with OA resulted in a doubling of TG accumulation (p < 0.05), compared to untreated cells (Table 1). We then assessed whether treatment with spice extracts was able to modulate TG accumulation in differentiated SW 872 cells, which were treated for 24 h and 48 h with spice extracts (all at 10 µg/mL). TG content did not change after a 24 h exposure to extracts. However, after 48 h, all extracts were able to significantly (p < 0.05) decrease TG accumulation (−11.3%/−18.5%). Resveratrol (10 µM), taken as active control, significantly (p < 0.05) reduced TG accumulation by 30.4% (Table 1). We then assessed whether treatment with spice extracts was able to modulate TG accumulation in differentiated SW 872 cells, which were treated for 24 h and 48 h with spice extracts (all at 10 µg/mL). TG content did not change after a 24 h exposure to extracts. However, after 48 h, all extracts were able to significantly (p < 0.05) decrease TG accumulation (−11.3%/−18.5%). Resveratrol (10 µM), taken as active control, significantly (p < 0.05) reduced TG accumulation by 30.4% (Table 1). results obtained by MTS assay and morphological analysis, all treatments with spice extracts were conducted for 24 h or 48 h at the concentration of 20 µg/mL or lower.

All Spice Extracts Reduce Triglyceride Accumulation in Differentiated Adipocytes
SW 872 cells were differentiated to adipocytes with 100 µM OA for seven days, according to a previously published protocol [8,9]. This treatment led to a marked intracellular lipids accumulation, as observed by ORO staining analysis ( Figure 1) and quantification of TG content (Table 1), indicating the acquisition of a mature adipocyte phenotype. Of interest, differentiation with OA resulted in a doubling of TG accumulation (p < 0.05), compared to untreated cells (Table 1). We then assessed whether treatment with spice extracts was able to modulate TG accumulation in differentiated SW 872 cells, which were treated for 24 h and 48 h with spice extracts (all at 10 µg/mL). TG content did not change after a 24 h exposure to extracts. However, after 48 h, all extracts were able to significantly (p < 0.05) decrease TG accumulation (−11.3%/−18.5%). Resveratrol (10 µM), taken as active control, significantly (p < 0.05) reduced TG accumulation by 30.4% (Table  1). We then assessed whether treatment with spice extracts was able to modulate TG accumulation in differentiated SW 872 cells, which were treated for 24 h and 48 h with spice extracts (all at 10 µg/mL). TG content did not change after a 24 h exposure to extracts. However, after 48 h, all extracts were able to significantly (p < 0.05) decrease TG accumulation (−11.3%/−18.5%). Resveratrol (10 µM), taken as active control, significantly (p < 0.05) reduced TG accumulation by 30.4% (Table 1).
We then explored the activation of the Akt signalling pathway, known to be associated with insulin receptors, under non-differentiated and OA-differentiated conditions and observed that 100 nM insulin treatment elicited a rapid and prolonged phosphorylation of Akt in OA-differentiated cells but not in non-differentiated cells ( Figure 3A). This insulindriven activation of Akt was not affected by a 24-h pretreatment of SW 872 cells with the three extracts that were found more effective in promoting glucose uptake (A. melegueta, Z. leprieurii, and T. tetraptera) ( Figure 3B).

Spice Extracts Affect Reactive Oxygen Species Production in Differentiated Adipocytes
The protective effect of antioxidants on cells is related to their ability to reduce the level of intracellular ROS generation. To assess whether spice extracts could reduce intracellular oxidative stress, intracellular ROS generation was quantified in differentiated SW 872 cells using the CM-H 2 DCFDA assay. Cells were differentiated with OA for seven days and treated or not for another 24 h with spice extracts before being exposed for 1 h to 500 µM H 2 O 2. Treatment with 500 µM H 2 O 2 alone produced in a 2.5-fold increase in intracellular ROS levels ( Table 2). When cells were pre-treated for 24 h with spice extracts (all at 10 µg/mL), ROS production induced by 1 h treatment with H 2 O 2 was reduced to a different extent by all spices except A. citratum, Z. leprieurii, and S. zenkeri, which were ineffective. Moreover, X. aethiopica and A. melegueta extracts significantly increased ROS production ( Table 2). In addition, the most effective extracts (X. parviflora, E. giganteus, and D. glomerata) were found to exert their ROS scavenging activity in a concentration-dependent fashion ( Figure 5). All plant extracts were used at 10 µg/mL. Data are expressed as % of H2O2-treated cells taken as 100; mean ±SD, n = 3. One experiment (n = 3) is shown as representative of 3 separated experiments, each in triplicate. Results are expressed as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001 (one-way ANOVA multiple comparison).
When cells were pre-treated for 24 h with spice extracts (all at 10 µg/mL), ROS production induced by 1 h treatment with H2O2 was reduced to a different extent by all spices except A. citratum, Z. leprieurii, and S. zenkeri, which were ineffective. Moreover, X. aethiopica and A. melegueta extracts significantly increased ROS production ( Table 2). In addition, the most effective extracts (X. parviflora, E. giganteus, and D. glomerata) were found to exert their ROS scavenging activity in a concentration-dependent fashion ( Figure  5).

Discussion
Nutraceutical approaches to prevent and treat cardiometabolic conditions are nowadays a relevant and promising research field [18], in some instances taking advantage of knowledge derived from traditional medicine. In the present study, we evaluated the effects of a set of plant extracts used in Cameroon as nutritional spices and medicinal agents [19,20] on cellular events related to the molecular pathophysiology of cardiometabolic diseases. In consideration that ectopic adipose tissue, especially when dysfunctional, is a well-known important player in cardiovascular and metabolic diseases [21], in the present study, we selected human SW 872 differentiated adipocytes as the model system [22,23]. SW 872 cells show, under basal conditions, an immature phenotype and constitutively express several genes involved in fatty acid metabolism, such as Figure 5. Concentration-dependent modulation of intracellular ROS release in SW 872 cells. The 3 most potent spice extracts were selected to evaluate the dose response. Concentration-dependent reduction of intracellular ROS production in SW 872 cells by selected spice extracts (Xylopia parviflora, Echinops giganteus, and Dichrostachys glomerata). Data are expressed as % of control taken as 100; mean ±SD, n = 3. One experiment (n = 3) is shown as representative of 3 separated experiments, each in triplicate. Results are expressed as mean ±SD. *** p < 0.001 (one-way ANOVA multiple comparison).

Discussion
Nutraceutical approaches to prevent and treat cardiometabolic conditions are nowadays a relevant and promising research field [18], in some instances taking advantage of knowledge derived from traditional medicine. In the present study, we evaluated the effects of a set of plant extracts used in Cameroon as nutritional spices and medicinal agents [19,20] on cellular events related to the molecular pathophysiology of cardiometabolic diseases. In consideration that ectopic adipose tissue, especially when dysfunctional, is a well-known important player in cardiovascular and metabolic diseases [21], in the present study, we selected human SW 872 differentiated adipocytes as the model system [22,23]. SW 872 cells show, under basal conditions, an immature phenotype and constitutively express several genes involved in fatty acid metabolism, such as lipoprotein lipase (LPL), cholesterol ester transfer protein (CETP), cluster of differentiation 36 (CD36), peroxisome proliferatoractivated receptor (PPAR)α, PPARγ and LDL receptor-related protein (LRP) [22]. For the purpose of this study, SW 872 were differentiated to mature adipocytes using OA, according to established protocols [9]. We observed an overall inhibitory action across all plant extracts regarding TG content of SW 872 cells since the massive TG accumulation induced by OA treatment was reduced by −11/−18% by all extracts after 48 h. Consistent with our data, it has been reported that lipid accumulation was significantly decreased in 3T3-L1 adipocytes by resveratrol [24], which was selected here as a positive control. All the tested spice extracts are rich in a variety of primary metabolites (glycerol, fructofuranose, glucopyranose, etc) and secondary metabolites (chlorogenic acid, catechin, pimaric acid, etc.) [5] that orchestrate the observed molecular responses.
The occurrence of some plant specificity in this effect is suggested by our previous observation that Adansonia digitata L. extracts did not affect TG accumulation in differentiated SW 872 adipocytes [8]. The development of obesity is accompanied by adipocyte hypertrophy and hyperplasia [25] leading to excess of lipid accumulation [26], and the observed effects may at least in part explain the actions of some of the tested plants in animal models. For example, extracts from A. melegueta seeds were found to reduce adipose tissue in obese mice [19], and high-carbohydrate and high-fat diet-induced obesity and diabetes in rats were attenuated by T. tetraptera extract [27]. Dysfunctional adipocytes, as observed in T2DM and obesity, also develop reduced glucose uptake [28][29][30]. In this study, T. tetraptera, A. melegueta and Z. leprieurii increased glucose uptake in a dose-dependent manner. Interestingly, in another in-vitro model, the HepG2 cells, T. tetraptera and A. melegueta, but not Z. leprieurii increased glucose uptake [7], underlining the relationship between the complex molecular composition of the spice extracts, the observed effect and the specificity linked to the tested cell model. In differentiated SW 872 cells, the tested spice extracts do not appear to modulate the Akt upstream pathway whether or not in the presence of insulin, suggesting that they may increase glucose uptake by promoting other molecular events, like glucose transporter translocation to the membrane. Moreover, insulin resistance, glucose uptake impairment and metabolic disfunction appear to be sustained by the secretion from dysfunctional adipocytes of several pro-inflammatory cytokines [31] as well as by the resultant ROS production [1,32]. Based on these considerations, we evaluated the secretory profile of IL-6 and IL-8, two NF-kB-dependent molecular mediators released in the context of chronic inflammation [7]. Among the 11 tested extracts, four (A. melegueta, D. glomerata, T. tetraptera and E. giganteus) were effective in significantly reducing (−29/−43%) IL-6 release. T. tetraptera, and D. glomerata were also found to similarly reduce IL-6 release by GES-1 gastric epithelial cells [6]. In addition, four spice extracts (X. parviflora, X. aethiopica, M. myristica, and Z. leprieurii) were able to reduce (−21/−36%) IL-8 release, whereas this was markedly increased by D. glomerata and A. citratum. Notably, D. glomerata extract showed a dichotomic effect, reducing IL-6 and increasing IL-8 secretion. It is important to highlight that the release of each cytokine was negatively modulated by fully different sets of plants. Although it is challenging to exactly establish the role of each extract and their metabolites in IL-6 and IL-8 modulation, it is well known that shogaol, gingerol, chlorogenic acid, pimaric acid, and other molecules identified in our extracts [5] are well known inhibitors of NF-κB [14,[33][34][35][36].
Furthermore, we assessed the effects of treatment with these Cameroonian spice extracts on H 2 O 2 -induced ROS production in differentiated SW 872 cells. All extracts, except A. citratum, Z. leprieurii and S. zenkeri were able to reduce ROS production. X. parviflora, E. giganteus and D. glomerata were the most effective and showed a dose-related activity. These antioxidant effects could be explained by the high content in phenolic compounds and the potent in-vitro antioxidant capacity previously reported by our group [7]. ROS production was however increased by X. aethiopica and A. melegueta extracts.
Taken together, the findings of the present study, summarized in Table 3, suggest that each of these spice extracts display a rather peculiar profile of activity on differentiated SW 872 adipocytes. This deserves further exploitation, in order to highlight in a more robust way the peculiar utilization of each spice extract to target specific functions (i.e., antioxidant vs. anti-inflammatory or promoting glucose uptake). Nutraceutical products with these activities may find their application in subjects with obesity, metabolic syndrome, T2DM and the related atherosclerotic cardiovascular disease risk, either alone, especially in milder conditions [37], or in combination with selected drugs in order to avoid drug dosage increase, prevent some adverse effects and increase the overall efficacy [4,38]. Interestingly, some pathophysiological mechanisms underlying these conditions, like oxidative stress and chronic low-grade inflammation, do not seem properly managed by the current pharmacology, and thus, well-characterized plant extracts could allow to specifically target them. This study has some limitations. OA-differentiated SW 872 cells resemble in some respects human adipocytes although this differentiation protocol is associated with peculiar molecular effects related to PPARγ activation, which therefore need to be taken into consideration in the interpretation of the obtained results. It is also important to underline that the extraction protocol utilized in this study [5] may differ from that used by others, making in some cases the comparison with other studies on the same plants difficult. This work, together with the others published in the last years, contributes to define and enrich the study of the biological activities of a set of Cameroonian spices, particularly in the context of dysfunctional adipocyte cell biology.

Conclusions
The results of this study show that the tested Cameroonian spice extracts display interesting activities on several molecular features of differentiated SW 872 human adipocytes. All extracts were able to reduce TG accumulation, while the ability to promote glucose uptake, reduce pro-inflammatory cytokines release, and counteract ROS production was limited to panels of three to six plant extracts (Table 3). Such variety of effects may be the basis for the development of nutraceutical products with very specific effects or combining more than one extract to achieve complementary effects. Moreover, T. tetraptera stands out as the most versatile plant since it was found to positively modulate most parameters. Since the 11 spice extracts showed rather variable viability profiles, with some extracts being toxic at concentrations just higher than the most effective doses (10-20 µg/mL), a word of caution should be spent regarding the potential toxicity of some plants when used at higher concentrations and/or prolonged treatments.
The findings of the present study, conducted in a human adipocyte in-vitro model, highlight some potential health properties of these Cameroonian spices and suggest the opportunity of further studies in the context of experimental and human cardiometabolic diseases.

Informed Consent Statement:
The data presented in this study are available on request from the corresponding authors.

Data Availability Statement:
The data presented in this study are available on request from the corresponding authors.