Peroxisome Proliferator-Activated Receptors as Molecular Links between Caloric Restriction and Circadian Rhythm

The circadian rhythm plays a chief role in the adaptation of all bodily processes to internal and environmental changes on the daily basis. Next to light/dark phases, feeding patterns constitute the most essential element entraining daily oscillations, and therefore, timely and appropriate restrictive diets have a great capacity to restore the circadian rhythm. One of the restrictive nutritional approaches, caloric restriction (CR) achieves stunning results in extending health span and life span via coordinated changes in multiple biological functions from the molecular, cellular, to the whole–body levels. The main molecular pathways affected by CR include mTOR, insulin signaling, AMPK, and sirtuins. Members of the family of nuclear receptors, the three peroxisome proliferator–activated receptors (PPARs), PPARα, PPARβ/δ, and PPARγ take part in the modulation of these pathways. In this non-systematic review, we describe the molecular interconnection between circadian rhythm, CR–associated pathways, and PPARs. Further, we identify a link between circadian rhythm and the outcomes of CR on the whole–body level including oxidative stress, inflammation, and aging. Since PPARs contribute to many changes triggered by CR, we discuss the potential involvement of PPARs in bridging CR and circadian rhythm.


Introduction
The circadian (circa-approximately; dian-day) clock is a system conserved from cyanobacteria to humans, which generates internal oscillations with a near 24 h periodicity coordinating bodily processes in an anticipatory fashion. In mammals, the circadian clock governs nearly all aspects of physiology and behavior, including hepatic metabolism, cardiovascular activity, gastrointestinal tract activity, blood pressure, endocrine secretions, body temperature, renal function, and sleep-wake rhythm [1][2][3][4]. The adaptation to the recurring external and internal changes results in diurnal activity cycles and the anticipation of such changes constitutes a survival advantage to an organism capable of adjusting its molecular, physiological, and behavioral pathways [1,2,5].
The expression of circadian genes oscillates in an organ-dependent manner, with roughly 9-20% of all transcripts, which may differ from one cell or tissue to another, exhibiting a rhythmic pattern [6][7][8][9]. In fact, some studies report up to 43% of protein-coding genes showing circadian oscillations [10]. Disruption of the circadian coordination either by mutations in circadian clock genes or environmental factors interferes with metabolic processes and creates hormone imbalance, During CR, the organism adapts to the limited available resources and synchronizes its metabolic activities to produce metabolic enzymes efficiently to maintain homeostasis, relying on phases of energy supply. CR likely recruits the circadian clock for this metabolic optimization. Changes in nutritional availability of metabolic cues are implicated in lifespan extension and attenuation of the aging-related phenotype in a clock-dependent manner [22,71,72]. Due to the here mentioned multiple interlinks between CR and the circadian clock, implicating a large number of metabolic pathways under the control of the circadian clock, some of the beneficial outcomes of CR likely take place via clock regulation. The crosstalk between the circadian clock pathways participating in CR mechanisms, such as the sirtuin, insulin/IGF, and mammalian target of rapamycin (m)TOR signaling pathways will be described in the following chapters. Importantly, as recently reviewed [73], PPARs are implicated in every aspect of molecular signaling and outcomes of CR.

Insulin Signaling in Metabolism and Circadian Rhythm Regulation
Insulin/IGF-1 pathway activity plays a major role in the control of aging [74][75][76][77][78] and in the beneficial effects of CR [79,80]. Insulin is a key regulator of glucose uptake and utilization in insulin-responsive tissues. Following food intake, increased blood glucose levels trigger pancreatic β-cells to secrete insulin. Free circulating insulin activates insulin receptors on the surface of target cells eliciting a signaling cascade initiated by the activation of insulin receptor substrates (IRS 1-4) followed by phosphorylation of phosphoinositide 3-kinase (PI3K), which manages metabolic response including PDK1 and Akt stimulation by phosphorylation. Akt signaling prompts glucose transporter 4 (GLUT4) to translocate to the cell membrane where it initiates cellular glucose uptake. Akt also stimulates the production of glycogen and inhibits gluconeogenesis. Moreover, Akt activates mTOR, which facilitates anabolic processes, while mTORC2 feeds back to regulate Akt [81]. Insulin signaling affects multiple downstream pathways including mitogen-activated protein kinase (MAPK), which controls growth, sterol regulatory element-binding protein 1 (SREBP-1), which stimulates the synthesis of lipid and cholesterol as well as the family of Forkhead (FOXO) transcription factors regulating metabolism and autophagy [82,83]. Inhibition of IGF-1/PI3K/Akt signaling contributes to the anti-cancer and DNA-repair activity of CR [84][85][86].
The direct interconnection between CR and circadian rhythms has been evinced by the fact that Igf-1 expression is regulated by both CR and the circadian clock [64]. Interestingly, Igf-1 expression is rhythmic and shows sexual dimorphism [64]. Furthermore, a genome-wide RNAi screen for genes that regulate cellular clock functions in human cells identified the insulin signaling pathway as the most overrepresented pathway [87]. Accordingly, the impact of CR on plasma IGF-1 and insulin level is compromised in mice deficient for Bmal1 [71]. Further, the postprandial release of insulin resets peripheral clocks by regulating the expression of core circadian genes. Insulin rapidly increases the expression of Per2 in insulin-sensitive tissues like the liver, muscle, or adipose tissue, but not the lung or brain [88]. Insulin secreted upon refeeding-after-fasting stimulates Per2 and reduces Rev-erbα expression in hepatocytes [27]. The capacity of insulin to initiate entrainment of the liver clock was demonstrated by the administration of insulin in cultured rat hepatocytes which acutely induced expression of Per1, Per2, and Dec1 [88]. Accordingly, inhibition of pathways downstream of insulin signaling, such as MAPK and PI3K, blocks the induction of the Per1 and Per2 clock genes [88].
Next to insulin, glucose is a circadian clock regulator. Glucose levels control AMPK activity, which phosphorylates and controls the stability of the CRY proteins [89]. In rats, glucose infusion during the light phase strongly induces expression of Per2 in the SCN and reduces Per2 expression in the liver [90]. Accordingly, a reduced level of glucose availability delays the light-induced phase shift [91]. Glucose also reduces the expression of Per1 and Per2 in fibroblasts in vitro [92].
Reciprocally, the circadian clock in the pancreas regulates insulin and glucagon production and secretion and their signaling in SCN via the autonomic nervous system [93][94][95][96]. Glucagon secretion

mTOR Signaling and the Circadian Rhythm
The mTOR pathway is a key effector pathway of CR and it is known for monitoring the availability of nutrients and regulating longevity. TOR is one of the Ser/Thr protein kinases from the family of phosphatidylinositol 3 (PI3) kinase-related kinases [111,112] and it functions as a key component of two complexes, mTORC1 and mTORC2 [111]. mTORC1 is sensitive to cellular energy levels, nutrient status, mitogenic signals, and oxygen levels and it is inhibited by rapamycin. mTORC1 signaling leads to the regulation of mRNA translation and autophagy. mTORC2 is not rapamycin sensitive and operates as a regulator of the cellular actin cytoskeleton [113,114]. The mTOR pathway integrates intracellular and extracellular physiological stimuli. In this pathway, the protein complex of tuberous sclerosis proteins 1 and 2 (TSC1 and TSC2) mediates upstream signals from growth factors, such as insulin and IGF-1 to mTORC1. Activation of mTOR mediates the phosphorylation of several executor proteins involved in mRNA translation and ribosome biogenesis, such as ribosomal S6 kinase 1 (S6K1) and eukaryotic initiation factor 4E-binding protein (4E-BP) [115][116][117]. mTORC1 downstream signaling controls autophagy and metabolism, including the glycolytic turnover and anabolic processes associated with the fed state including lipogenesis [118][119][120][121], cholesterol synthesis via activation of SREBP-1/2 [118,122,123], and protein synthesis [124,125]. mTOR activity in vivo is induced by the abundance of nutrients and gradually decreases during fasting. However, food-independent rhythmicity in activity and expression of the mTORC1 complex members has been observed in the SCN and liver, cardiac and skeletal muscles, adipocytes, and retinal photoreceptors but not in the intestine or lung [126][127][128][129][130][131][132][133][134]. In the mouse brain, mTORC1 activities exhibit daily alterations in the arcuate nucleus, hippocampus, and the frontal cortex [130,135,136], all regions that manage circadian rhythms, feeding, learning, memory, and emotions. The oscillations of mTOR activity are delimited by internal and external regulators [137]. In the brain of Drosophila, TOR rhythms are found particularly in the ventral lateral neurons [138,139]. Neuronal TORC1 and AKT Nutrients 2020, 12, 3476 7 of 59 signaling have been shown to drive behavior [138]. Also, in the SCN, mTORC1 signaling is activated by light and controls behavior in a circadian manner [126,127,140]. Brief light exposure of mice during the night, but not during the day, triggers instant phosphorylation of the mTOR translation effectors S6K1, S6 ribosomal protein (S6), and translational repressor 4E-BP1 [126]. A KO of 4E-BP1 in mice leads to a higher amplitude of molecular rhythms in the SCN, increased capacity for re-entrainment to a shifted light/dark cycle, and higher resistance to the disruption of rhythm by constant light [140]. In vivo, infusion of the inhibitor of mTOR1 rapamycin leads to an attenuation of the phase-delaying effect of early-night light. Equally, disruption of mTOR during the late-night augments the phase-advancing effect of light. At both the early-and late-night time points, abrogation of mTOR signaling leads to a significant attenuation of light-induced PER protein expression [141]. Conversely, constitutive activation of mTOR in Tsc2-deficient fibroblasts alters the dynamics of clock gene rhythmicity and raises levels of principal clock proteins, including CRY1, BMAL1, and CLOCK [142]. Heterozygous mTor KO mice present a lengthened circadian period of locomotor activity rhythms both in constant darkness and constant light [142]. mTOR inhibition lengthens the period and dampens the amplitude of circadian clock proteins, whereas mTOR activation shortens the period and augments amplitude in hepatocytes, adipocytes, and human U2OS cells [142,143] In the liver, fasting/feeding cycles regulate the energy sensor AMPK and mTORC1 [28,129,130] independently of CRY1 and CRY2 but partly relying on BMAL1 [137]. S6K1 rhythmically phosphorylates BMAL1 at an evolutionarily conserved site to activate it for modulation of mRNA translation and protein synthesis. Therefore, protein synthesis rates show circadian oscillations that are dependent on BMAL1 [131], which deficiency increases mTORC1 activity both in vivo and in vitro [130]. Increased mTOR signaling correlates with faster aging while treatment with rapamycin extends the lifespan of prematurely-aging Bmal1 KO mice by 50% [130]. Therefore, increased BMAL1 activity likely contributes to lower mTORC1 activity in CR. In summary, the regulation of mTOR downstream and upstream of the circadian rhythm connects daily oscillations with sensing of the nutrient status ( Figure 2).

AMPK as an Energy Sensor and Executor of Circadian Metabolic Activities
AMPK serves as a key energy sensor of the AMP:ATP and ADP:ATP ratios. AMPK, activated at low levels of ATP, suppresses energy-consuming anabolic pathways and promotes catabolism resulting in the recruitment of stored energy to reestablish the ATP levels [ The circadian clock is paired with cellular metabolic fluctuations via nutrient-sensing pathways. While the expression of six of the transcripts encoding subunits of mammalian AMPK does not vary during the day, that of the AMPKβ2 subunit is eight times higher in the middle of the day than it is at night. AMPKβ2 determines the cellular localization of the heterotrimeric AMPK Nutrients 2020, 12, 3476 8 of 59 complex [164]; therefore, the presence of the AMPKα1 subunit in the nucleus increases at the time of day when AMPKβ2 expression peaks [89]. The rhythmic hepatic activity and nuclear localization of AMPK correlate inversely with CRY1 levels, which is likely due to the fact that stimulation of AMPK leads to phosphorylation and degradation of CRY [89]. Further, activation of AMPK by 5-aminoimidazole-4-carboxamide ribonucleoside (AICAR) or metformin in mouse livers causes a phase shift of the clock, and animals in which the AMPK pathway is genetically disrupted show alterations in peripheral clocks [89,165]. Moreover, acute AICAR stimulation alters the expression of clock genes in WT mice but not in mice lacking the AMPKγ3 regulatory subunit [166]. Genetic disruption of either AMPKα1 or AMPKα2 subunit dampens the rhythm of body temperature, the free-running activity, changes the circadian pattern of core clock gene expression in mice in an isoform-and tissue-specific manners [165]. In mouse muscles, AMPK regulates the expression patterns of the circadian genes Cry2, Rev-erbα, and Bhlhb2 (basic helix-loop-helix domain containing class B 2) [166]. Moreover, AMPK is capable of phosphorylating casein kinase ε (CKIε) and thereby increases its enzymatic activity, indirectly leading to a destabilization of PER2 [167]. PGC-1α, which co-activates the RORs and consequently stimulates the expression of Bmal1 and Rev-erbα, is phosphorylated by AMPK [148,168,169]. PGC-1α is required for cell-autonomous clock function [169] and PGC-1α KO mice show an abnormal diurnal rhythm of physical activity, body temperature, and metabolic rate, due to disrupted expression of clock genes and genes involved in energy metabolism. Besides the direct impact on ATP levels, AMPK affects the energy status by promoting feeding through signaling in the hypothalamus as well as by adjusting circadian metabolism [170,171]. AMPK may thereby mediate the influence of fasting/feeding cycles on the circadian clock ( Figure 2).
The rhythmic acetylation of BMAL1 and acetyl-histone H3 Lys9/Lys14 at circadian promoters correlates with SIRT1 HDAC activity that is regulated in a circadian manner. Therefore, genetic or pharmacological inhibition of SIRT1 activity causes disturbances in the acetylation of H3 and BMAL1 and the circadian rhythm [206]. Moreover, the circadian transcription factor CLOCK has histone acetyltransferase (HAT) activity, and SIRT1 HDAC activity counteracts the HAT activity of CLOCK [201,206]. Another circadian protein, REV-ERBα regulates pancreatic glucagon secretion via the AMPK/nicotinamide phosphoribosyltransferase (NAMPT)/SIRT1 pathway [94]. Further, the expression of NAMPT, a rate-limiting enzyme involved in NAD + production through the salvage pathway, is regulated by the BMAL/CLOCK heterodimer. Consequently, NAD + levels exhibit rhythmic daily oscillations [207,208]. Moreover, by being recruited to the Nampt promoter, SIRT1 contributes to the circadian synthesis of its own coenzyme [208]. Inhibition of NAMPT promotes fluctuations of Per2 by releasing CLOCK:BMAL1 from suppression by SIRT1. In turn, CLOCK binds to the Nampt promoter and stimulates its activity, thereby contributing to a feedback loop comprising NAMPT/NAD + and SIRT1/CLOCK:BMAL1 [207].
SIRT3 also interacts with the circadian rhythm. It sets the pace in the acetylation and activity of oxidative enzymes and consequently respiration in isolated mitochondria. Bmal1 KO mice have significantly decreased SIRT3 activity, which affects mitochondrial oxidative function, and supplementation with nicotinamide mononucleotide (NMN), a NAD + precursor, restores SIRT3 function and enhances oxygen consumption in these animals [209]. Importantly, the rhythm of cyclic global protein acetylation dampens with aging in mice [70]. CR regulates SIRT1 activity and therefore it modulates the circadian acetylation of AceCS1, a pathway controlling rhythmic nucleocytoplasmic acetyl-CoA production [210,211]. Consequently, CR rescues the hepatic protein acetylation rhythm over the day/night cycle in mice. Accordingly, the circadian transcriptome of CR-mediated effects on circadian reprogramming and SIRT1-specific transcriptome overlaps [70] indicating a pivotal role of SIRT1 in connecting CR and the circadian rhythm ( Figure 2). Abbreviations: AMPK-adenosine monophosphate (AMP) activated protein kinase; CKIε-casein kinase ε; CLOCK-circadian locomotor output cycles kaput; Dec1-differentially expressed In chondrocytes 1; IGF-1-insulin-like growth factor 1; IRS-insulin receptor substrates; LKB1-liver kinase B1; mTOR-mammalian target of rapamycin; NAD-nicotinamide adenine dinucleotide; NAMPT-nicotinamide phosphoribosyltransferase; SIRT1-Sirtuin 1; SCN-suprachiasmatic nucleus, S6K1-ribosomal protein S6 kinase 1. The circled Ac indicates acetylation and circled P phosphorylation.

PPARs
The ligand-dependent receptors PPARs form a nuclear receptor subfamily which comprises three isotypes, PPARα (NR1C1), PPARβ/δ (NR1C2), and PPARγ (NR1C3) [212][213][214]. PPARs play roles in multiple processes including FA and eicosanoid signaling, cell proliferation and differentiation, tissue repair and remodeling, bone formation, insulin sensitivity, glucose and lipid metabolism. Synthetic ligands [thiazolidinediones (TZD) and fibrates] of PPARs are commonly used in the therapy of glucose, and lipid disorders as well as in the prevention and treatment of cardiovascular and metabolic diseases [215][216][217]. Diverse FAs, phospholipids, prostacyclins, prostaglandins, and leukotrienes serve as natural ligands of PPARs [218,219] and also are involved in other biological processes, which indicate a potential link between PPARs and nutrition, metabolism, and inflammation. In response to CR, PPAR expression patterns vary vastly in different tissues and organs. In brief, the expression of PPARα, -β/δ, and -γ in the heart, PPARα in white adipose tissue (WAT), and PPARγ in the liver remains unchanged during CR. On the contrary, the expression of PPARβ/δ in the liver, PPARα in the spleen, and PPARα, -β/δ, and -γ in muscle is decreased, whereas that of PPARα in the liver and intestinal epithelium is increased [220][221][222][223][224][225][226][227][228]. In rat kidneys, 60% CR prevents age-related reduction in PPAR activity [229].
PPARs contribute to CR-related outcomes by regulating many pathways, all of which we have reviewed recently [73], such as mTOR, insulin signaling, AMPK, SIRT, inflammation, oxidative stress, mitochondrial function, energy metabolism, aging, hunger, and microbiota composition. Simultaneously, PPARs act as molecular connectors joining clock genes and specific rhythmic metabolic outputs. All PPARs exert their functions in a circadian manner and their expression is regulated by both core clock genes and clock-controlled genes. Moreover, PPARs and the core clock genes cross-regulate each other at the transcription level. Additionally, CLOCK/BMAL1 and PPARs synergistically regulate gene expression as CLOCK/BMAL1 heterodimers increase the transcriptional activity of some PPAR target genes. Upon PPAR response element (PPRE) removal from the promoter of these genes, CLOCK/BMAL1 loses its transcriptional effects [230]. Finally, it is important to bear in mind that there are multiple interactions between each PPAR isotype and the circadian rhythm ( Figure 3).

PPARα
Most studies on the implication of PPARs in CR and circadian rhythm are about PPARα, which is particularly important for lipid metabolism and is expressed at relatively high levels in organs with significant FA catabolism, such as the liver and brown adipose tissue (BAT) [231]. PPARα synchronizes metabolism by regulating genes associated with FA transport, peroxisomal and mitochondrial β-oxidation, ketogenesis, and gluconeogenesis [218]. Considering the adaptational role of PPARα, its ligands can be seen as mimetics of CR [232]. As a sensor of the energy status, PPARα adjusts metabolic processes to both, scarcity and abundance of nutrition.
PPARα modulates an important aspect of CR response, hunger perception. Endocannabinoid oleoylethanolamide (OEA), a ligand of PPARα is particularly well recognized for appetite regulation [251]. OEA is synthesized in enterocytes in response to fat consumption and is increased by sympathetic activity and bile acids that activate N-acylphosphatidylethanolamines hydrolyzing phospholipase D (NAPE-PLD) [252,253]. OEA has an anorectic effect via PPARα activation and it reduces meal size or delays meal intake, which results in body weight loss [251,[254][255][256]. OEA acts peripherally via afferent sensory fibers of the vagal nerve in the intestine. This signaling promotes the secretion of the neuropeptide oxytocin and satiety by increasing the expression of c-fos in the nucleus solitary tract of the brainstem and the paraventricular nucleus of the hypothalamus [257,258]. OEA synthesis is increased following gastric bypass surgery that requires intestinal PPARα expression to reduce food intake [259]. Moreover, a single nucleotide polymorphism (SNP) in PPARα is associated with an increased risk of high altitude appetite loss [260], and activation of hypothalamic PPARα normalizes food intake in hypophagic Fas KO mice [261]. Unexpectedly, challenging hepatic Pparα KO mice with carbohydrates revealed a PPARα-dependent glucose response for FGF21. This effect is associated with an increased preference of these mice for sucrose [262]. Finally, dietary protein restriction increases FGF21 expression in human and rodent plasma, an effect which depends on increased phosphorylation of eukaryotic initiation factor 2α (eIF2α) in the liver, eIF2α kinase general control nonderepressible 2 (GCN2), and PPARα signaling [263].

PPARα and Molecular Effectors of CR
PPARs interact with all of the major pathways managing the response to CR. The diverse interactions between mTOR and PPARs impact FA synthesis, glucose metabolism, oncogenesis, and the immune system. mTORC1 adjusts ketone body production upon fasting [264] and mice with constitutive hepatic activation of mTORC1 present low PPARα levels and are not able to induce ketogenesis in response to fasting [264]. In the fed state, the insulin-dependent PI3K pathway activates mTORC1, which inhibits PPARα activity and ketogenesis [264]. Additionally, insulin-dependent phosphorylation and thus inhibition of cytoplasmic FA synthase (FAS), which is mediated by mTORC1, limits the synthesis of PPARα ligands. Conversely, when mTOR is inhibited during fasting, FAS generates endogenous PPARα ligands [265]. In the intestine, mTOR activity increases with age, which is accompanied by inhibition of PPARα. Consequently, levels of the primary Wnt feedback inhibitor, Notum increase, and Wnt signaling is reduced leading to the diminished regenerative capacity of stem cells in the Paneth cell niche [266]. Similarly, in the livers of old mice, elevated mTORC1 activity [264] coincides with diminished activity of PPARα and reduced ketogenesis [267][268][269]. Unsurprisingly, mTORC1 inhibition prevents aging-induced loss of PPARα activity and ketone body production [264]. Finally, CR induces autophagy in the liver and other organs by integrating, mTOR, and PPARα functions [270,271].
As already mentioned, AMPK and PPARα both sense the energy status and adjust metabolism to nutritional changes. PPARα stimulates the expression of proteins involved in restoring ATP levels and therefore regulates AMPK activity. Moreover, PPARα and AMPK act to regulate long-term and short-term FA oxidation, respectively. Therefore, during fasting, as glucose levels drop and FA levels rise, AMPK and PPARα coordinate their actions. High intracellular AMP levels induce AMPK, which stimulates mitochondrial FA uptake while PPARα enhances the hepatic β-oxidation capacity [218,265,272,273]. The activation of PPARα by AMPK occurs in several tissues including muscle, liver, and pancreas [274][275][276][277][278][279][280]. Agonist activation of PPARα also activates AMPK showing that the stimulation of AMPK and PPARα is reciprocal [281][282][283].

PPARα in Circadian Rhythm
PPARα is expressed according to a diurnal rhythm in mouse liver, heart, kidney, and, to a smaller extent, in the SCN [288,289]. It is directly linked with the circadian clocks as it maintains the circadian oscillation of the Bmal1 gene in the brain and muscle by binding to its promoter ( Figure 3). Accordingly, fenofibrate, a PPARα ligand, promotes circadian clock gene expression in cell culture, up-regulates hepatic Bmal1 in vivo, and Pparα KO mice exhibit altered hepatic circadian expression of Bmal1 and Per3 [290]. Reversely, BMAL1 is an upstream regulator of Pparα expression [290]. Similarly, the CLOCK-BMAL1 heterodimer transactivates Pparα. CLOCK interacts with the E-box-rich region of the Pparα promoter and, in homozygous Clock KO mice, the hepatic circadian expression of Pparα is abolished [291]. Interestingly, Rev-erbα is also a PPARα target gene [292].
Besides its transcription regulatory role, PPARα also interacts directly with clock proteins. PER2 interacts with PPARα and serves as its transcriptional coregulator. Consequently, PER2 is rhythmically bound at the promoters of PPAR target genes in vivo [293]. Considering multiple interactions between PPARα and circadian clock proteins it is important to reflect on the impact of medical drugs, mostly lipid-lowering ones, acting as PPARα ligands. Fenofibrate increases transcription and resets rhythmic expression of BMAL1, PER1, PER3, and REV-ERBα in mouse livers [290] and cultured hepatocytes [292]. Bezafibrate was shown to phase-advance the circadian expression of BMAL1, PER2, and REV-ERBα in the cortex, liver, and fat of mice but without affecting the SCN [294]. Additionally, bezafibrate advances the active phase of mice under light-dark conditions in a photoperiod-dependent manner; therefore, it is involved in the entrainment of the circadian clock to environmental light-dark conditions [294,295].
Lipid homeostasis is controlled by the circadian clock and disruption of rhythmicity results in dyslipidemia and obesity in various clock mutant mouse models. Rev-Erbα KO mice exhibit elevated VLDL triglyceride levels [296], in Bmal KO mice triglycerides oscillations are dampened in plasma [98], Clock KO mice show hyperlipidemia and hepatic steatosis [297] whereas Per2 KO mice have reduced whole-body fat, total triglycerides as well as FAs in plasma [298]. Accordingly, around 15% of total metabolites undergo diurnal regulation in human plasma with 75% of them being lipids [299]. Similarly, about 13% of lipid metabolites exhibit circadian changes in human plasma [300], with a high prevalence of diglycerides, and triglycerides peaking in the middle of the day. Consistently, the oscillation of triglycerides in the rat plasma corresponds to the feeding cycle [301]. In mouse liver, 17% of quantified lipids display circadian rhythmicity with all triacylglycerols (TAG) reaching their peak levels around CT8 which parallels PPARα peak expression in the liver. Surprisingly, Per1/2 KO mice show variations of a similar fraction of lipids, however, with a different composition than in the WT mice. Moreover, PPARα retains its circadian expression upon Per1/2 deletion [302]. In the same way, adipose tissue (BAT, iWAT, and eWAT) shows a cyclic expression of the circadian oscillator genes including Npas2, Bmal1, Per1-3, and Cry1-2, in combination with Pparα fluctuations [303].
Consistent with its role in the circadian regulation of lipid metabolism, PPARα controls numerous genes implicated in lipid and cholesterol metabolism, and energy homeostasis. PPARα-driven cardiac regulation of enzymes involved in FA β-oxidation in the heart ensures metabolic homeostasis and heart performance [304,305]. The expression of FGF21 playing important roles in adaptation to fasting, such as lipolysis and ketogenesis is under the circadian influence of PPARα [306]. Many of the metabolic genes, such as Srepb, Fas, and β-hydroxy β-methylglutaryl-CoA reductase (Hmg-CoAR), display daily fluctuations in mouse liver. The rhythmical expression of Fas and Hmg-CoA over the day presents a pattern that is opposite of that of Pparα. In the Pparα KO mice, the rhythmical expression of lipogenic and cholesterogenic genes is dampened or eradicated. However, compared to WT mice, these changes are observed only during the active phase when food intake is high [307].
PPARα is considered one of the marker genes of CR. Its expression is altered in CR in multiple organs and it likely mediates the response to metabolic changes as well as metabolic switch to low-energy conditions including mobilization of energy stores. PPARα is also inseparably entwined in circadian clock control. Importantly, CR significantly enhances the circadian activity of PPARα and, thus the oscillatory expression of its target genes [69]. Consequently, PPARα is one of the best documented examples of a factor bridging CR and circadian rhythm.

PPARβ/δ
In contrast to the expression pattern of PPARα and PPARγ, PPARβ/δ is more ubiquitously expressed, with particularly high levels in several regions of the brain, kidneys, liver, skin, and gastrointestinal tract [308,309]. It is involved in cell proliferation, differentiation, tissue repair, placenta, and gut development, as well as energy homeostasis especially in muscle [280,[310][311][312][313][314][315][316]. PPARβ/δ stimulates energy expenditure by enhancing FA oxidation in skeletal muscle and adipose tissue, thereby protecting against diet-induced obesity and insulin resistance [317][318][319][320]. Moreover, hepatic deletion of PPARβ/δ reduces, while its activation increases, FA uptake in muscles, pointing at coordinated production and utilization of lipids in functionally interacting organs [321]. Exercise-induced glycogen exhaustion triggers PPARβ/δ activity in rat muscle stimulating changes in energy sources [322]. PPARβ/δ is particularly well known for increasing exercise capacity by promoting the switch to type I muscle fibers, which stimulates mitochondrial activity and fat oxidation resulting in improved endurance [323,324]. Furthermore, PPARβ/δ modulates feeding responses and its neuronal deletion leads to increased susceptibility to diet-induced obesity, elevated fat mass and decreased lean mass on low-fat diet, accompanied by abnormal responses to fasting [325].

PPARβ/δ and Molecular Effectors of CR
Only a few studies have documented a connection between mTOR and PPARβ/δ thus far. PPARβ/δ may impact mTOR activity by controlling FA metabolism and the production of phosphatidic acid, which directly activates the mTOR complex [326]. In non-small cell lung carcinoma (NSCLC) cells, the expression of PPARβ/δ is activated by nicotine through PI3K/mTOR [327], whereas GW501516, a PPARβ/δ agonist stimulates cell proliferation by inhibiting the expression of phosphatase and tensin homolog deleted on chromosome 10 (PTEN), which signals downstream to mTOR [328], picturing an interplay between the two pathways.
In models of metabolic syndrome [329], diabetes [330], and in overweight subjects [331], the GW501516 agonist of PPARβ/δ lowers plasma glucose and/or insulin levels. One of the mechanisms of energy status sensing by PPARβ/δ involves activation of cytosolic phospholipase A2 (cPLA 2 ) by glucose surplus, which leads to hydrolysis and peroxidation of arachidonic and linoleic acid, producing PPARβ/δ ligands [332]. Activated PPARβ/δ represses the β-cell mass and insulin secretion [333]. It prevents insulin resistance in adipocytes [334], human liver cells [335], and skeletal muscle cells [335,336]. Joint complementary effects of PPARβ/δ in distinct tissues involving its impact on carbohydrate catabolism in the liver and β-oxidation in muscle results in improved metabolic homeostasis and insulin sensitivity [337].
AMPK and PPARβ/δ interact directly in the muscle to enhance exercise performance [338]. AICAR, an activator of AMPK, increases endurance, but the combination of AICAR and GW0742, an agonist of PPARβ/δ, significantly boosts the activity of the receptor and rises all running parameters. The mechanism behind involves a lowering in carbohydrate utilization with a parallel shift to fat as the main energy source in fatigued muscles [339]. Therefore, agonists of AMPK and PPARβ/δ are considered exercise mimetics [340].

PPARβ/δ in Circadian Rhythm
In mice, the expression of Pparβ/δ mRNA oscillates daily in brown adipose tissue and muscle [288]. PPARβ/δ regulates the diurnal expression of lipogenic genes including acetyl-CoA carboxylase 1 and 2 (Acc1 and Acc2), Fas, and stearoyl-CoA desaturase-1 (Scd-1), during mouse feeding cycle [321]. Moreover, hepatic Pparβ/δ is a target of mir-122 and likely mediates the impact of mir-122 on cholesterol and lipid metabolism. Importantly, mir-122 transcription is rhythmically regulated by REV-ERBα [347]. Finally, PPARβ/δ expression rhythmically changes in hamster SCN where it modulates glutamate release. Treatment with the PPARβ/δ agonist L-16504 enhances the phase delay of the locomotor response induced by a light pulse. This observation suggests that PPARβ/δ is mediating entrainment of the circadian clock by light ( Figure 3) [348].

PPARγ
PPARγ is primarily known as a master regulator of adipogenesis, a target of insulin-sensitizing, and anticancerogenic therapies [349][350][351]. It has been shown to impact several types of cancers by reducing cell proliferation [352][353][354], stimulating cell differentiation [352,355], triggering apoptosis [352,353,356], and inhibiting angiogenesis [357]. PPARγ contributes to several processes including adipogenesis and FA storage, inflammation, and lipid and glucose metabolism [349,358,359], long-chain FA processing in the intestinal epithelium [360], and control of adiposity via the sympathetic nervous system [361]. Classical full PPARγ agonists TZDs such as rosiglitazone (Avandia) and pioglitazone (Actos) are used to be applied in the therapy of metabolic diseases [362].
Remarkably, dual PPARα/γ agonists have beneficial effects on both lipid and glucose metabolism. In addition to their antidiabetic properties, they also are hypolipemic, anti-inflammatory, hypotensive, and antiatherogenic, and exert anticoagulant effects [217,[363][364][365]. Multiple natural compounds stimulate PPARγ expression and activity. They include nutrients, most importantly FAs and their metabolites. Furthermore, glutamine, curcumin, capsaicin, ginsenosides, and vitamin E impact PPARγ activity indirectly [366]. Importantly, bacterial metabolites and bacterial by-products [367][368][369], and specific bacterial strains in the gut microbiota also increase the expression and activity of PPARγ [368,370,371]. A common side effect of treatment with TZDs is weight gain. HFD-related weight gain partly depends on the effect of neuronal PPARγ signaling which leads to limited thermogenesis and increased food intake [372,373]. Moreover, PPARγ plays role in regulating food intake by stimulating adipogenesis since WAT secretes endocrine and paracrine satiety mediators like leptin, adiponectin, and resistin [374].

PPARγ and Molecular Effectors of CR
Of all PPARs, the interplay of PPARγ with CR pathways has resulted in the richest set of evidence for an important role of PPARγ in the outcomes of CR. For instance, genetic variation in the Pparγ gene and its target gene Acsl5 are implicated in the extend of weight reduction upon CR [375]. In particular, six Pparγ SNPs correlate with weight loss under CR [376]. The Pro(12) Ala substitution of Pparγ reduces the binding affinity for PPRE and therefore, this PPARγ variant has a weaker transcriptional activity [377,378]. The Pparγ (12)Ala allele confers resistance to CR-induced weight loss in obese women [375] whereas, following CR, female homozygotes for Ala(12)Ala regain more body weight than Pro (12) Pro homozygotes [379]. Further, Pparγ polymorphism is linked with BMI changes due to fat intake [380,381], diet FA composition [382], and plasma TG response to ω3-FA supplementation [383].
PPARγ is well known as a regulator of insulin sensitivity and TZD drugs ameliorate insulin resistance by reducing plasma glucose and they improve the lipidemic profile in type 2 diabetes (T2D) and in obese subjects without diabetes [384]. TZDs also sustain the function of pancreatic β-cell thereby reducing T2D incidence [385,386]. In addition to TZDs, several other PPARγ agonists including FMOC-L-leucine (F-L-Leu) [387], INT131 besylate [388], quercetin, and kaempferol [389,390] improve insulin and glucose management. PPARγ exerts its insulin-sensitizing effects in several ways. It promotes functional WAT necessary for proper glucose homeostasis as evinced by insulin resistance upon both partial and generalized lipodystrophies [391,392] as well as by improved glycemic control following PPARγ-triggered fat mass generation [393]. Moreover, the magnitude of PPARγ-elicited insulin sensitization is proportional to the reduction of lipid accumulation in skeletal muscle [394]. Accordingly, various point mutations in the Pparγ gene, which result in familial partial lipodystrophy are accompanied by severe insulin resistance [395][396][397][398][399]. Consistently, dominant-negative mutations in human Pparγ causes metabolic syndrome, insulin resistance, and diabetes at a young age [400,401]. Further, the TZD Pioglitazone impacts the insulin signaling pathway to improve insulin sensitivity [402]. In fact, PPARγ increases the expression of factors in the insulin signaling pathway, including IRS-1 [403], IRS-2 [404], the p85 subunit of PI3K [405], and Cbl-associated protein (CAP) [406,407]. In muscle cells and adipocytes, PPARγ activation increases the expression and translocation of GLUT1, GLUT2, and GLUT4 to the cell membrane stimulating glucose uptake and lowering plasma glucose concentrations [408][409][410]. Further, PPARγ manages the intercellular glucose levels by regulating the expression of genes connected with insulin-stimulated glucose disposal [403][404][405][406][407]. The insulin-sensitizing effect of PPARγ ligands relies on a reduction in local and systemic cytokine production [411] and counteracting tumor necrosis factor α (TNFα) effects [412]. Moreover, PPARγ enhances the plasma levels of adipocytokines, among them adiponectin, which improves insulin sensitivity together with increased free FA oxidation and reduced gluconeogenesis [413,414]. In hepatocytes, Akt2 a downstream factor of insulin signaling stimulates the expression and activity of PPARγ leading to stimulation of glycolysis and lipogenesis [415]. In addition, an interaction between FOXO1 and PPARγ affects insulin sensitivity in adipocytes. FOXO is a transcriptional repressor of Pparγ. It binds to its promoter and may also reduce the transcriptional activity of PPARγ by direct protein-protein interaction [416][417][418].
PPARγ, similarly to what we discussed for PPARα, also controls autophagy, which is the major cellular process associated with mTOR. PPARγ activation increases autophagy, but its inactivation lessens it [438,439]. Cell cycle arrest and stimulation of autophagy are two major processes by which PPARγ stops cancer cell progression, as evinced in the colon [440], breast [441,442], bladder [443], and adrenocortical [444] cancer cell lines. Contrariwise, in neurons [445,446] and macrophages [447] PPARγ shows anti-autophagic properties.

PPARγ in Circadian Rhythm
As an important factor in response to CR and metabolic processes, PPARγ is inevitably implicated in circadian rhythm regulation (Figure 3). Bmal1 [468], Rev-Erbα [469], and Rorγ [470] are induced by PPARγ. 15-deoxy-Delta12,14-prostaglandin J2 (15d-PGJ2), a natural ligand of PPARγ, promotes the rhythmic expression of endogenous clock genes in NIH3T3 cells. Moreover, 15d-PGJ2 transiently induces Cry1, Cry2, and Rorα expression. However, the 15d-PGJ2-induced entrainment signaling pathway seems to be PPARγ-independent [471]. Similarly, PGC-1α, which interacts with PPARγ, is rhythmically expressed in mouse liver and muscle and it stimulates the expression of Bmal1 and Rev-erbα [169]. PGC-1α KO mice are affected in their locomotor activity and are characterized by an altered daily oscillation of body temperature and energy metabolism, in association with the abnormal expression pattern of metabolic and clock genes [169].
In the whole-body Pparγ deletion induced in the adult mouse (EsrCre/flox/TM mouse), circadian variations in oxygen consumption, CO 2 production, food and water intake, locomotor activity, and cardiovascular parameters are repressed and the rhythmicity of the canonical clock genes in adipose tissues and liver, but not skeletal muscles or the kidney, are impaired indicating disturbed circadian rhythms at both behavioral and cellular levels [472]. Pparγ shows circadian expression patterns in mouse fat, blood vessels, and liver [288,468]. The Pparγ expression in the aorta is robustly rhythmic with a more than 20-fold change during the day/night cycle [468]. Pparγ Tie2Cre/flox and SM22Cre/flox KO mice showing Pparγ deletion in the endothelial and vascular smooth muscle cells respectively, are characterized by reduced circadian oscillations in heart rate and blood pressure, which is associated with diminished changes in urinary norepinephrine and epinephrine excretion. In these mice, the rhythmicity of the canonical clock genes, including Bmal1 is compromised [468]. In addition, pioglitazone has been shown to restore the nocturnal blood pressure decline and transform blood pressure from a nondipper to a dipper type in type 2 diabetic patients [473].
Similarly, like PPARγ, the circadian clock is important for adipose tissue functions and fat metabolism. The core clock proteins regulate all aspects of lipid management by cyclic repression of several genes, including those coding for rate-limiting lipolytic enzymes. Consequently, the disruption of the circadian clock causes aberrant dietary lipid absorption, lipid transport, fat storage, and triglyceride levels [474]. The expression of Rev-Erbα is stimulated by PPARγ during adipogenesis in rat perirenal and epididymal adipose tissues, as well as in 3T3-L1 adipocytes. REV-ERBα facilitates the expression of PPARγ target genes and, accordingly, ectopic expression of Rev-Erbα in 3T3-L1 cells potentiates rosiglitazone PPARγ agonist-induced adipocyte differentiation [469,475]. Therefore, double KO of Rev-erbα and Rev-erbβ causes deregulated lipid metabolism [476]. Similarly, Rorγ is induced during adipocyte differentiation following the induction of Pparγ in D1 and 3T3-L1 cells and functions as an active transcription factor [470]. Also, BMAL1 via WNT signaling controls circadian regulation of adipocyte differentiation [477,478] and embryonic fibroblasts from Bmal1 KO mice fail to differentiate into adipocytes, whereas over-expression of Bmal1 in adipocytes increases lipogenesis [477]. Nocturnin, which is controlled by the clock, manages the circadian dietary fat uptake and clock KO mice exhibit altered dietary lipid trafficking [479], whereas PPARγ also plays a role in the uptake of long-chain FA in the intestine [360]. Furthermore, CR increases the amplitude of the circadian clock gene timeless (Tim) cycling, which has been implicated in daily fluctuations of several medium-chain triglycerides under CR. Overexpression of Tim in peripheral tissues improves oscillatory amplitude, enhances fat metabolism, which extends lifespan under ad libitum conditions [480].
HFD induces robustly the hepatic daily expression pattern of Pparγ. In HFD-fed mice, the levels of PPARγ protein are elevated, but do not show daily oscillations. Interestingly, however, nuclear and chromatin-bound PPARγ shows a significant circadian oscillation in these mice. Accordingly, PPARγ targets gain rhythmicity after acute HFD feeding [481]. Furthermore, the HFD-related hepatic fluctuations of PPARγ activity are driven by microbial communities in the gut as documented by antibiotic treatments that repress PPARγ-driven transcription in the liver. Therefore, HFD-induced remodeling in the gut microbiota mediates PPARγ-driven modulation of the host liver clock, resulting in adjusted circadian transcription [482].
and chromatin-bound PPARγ shows a significant circadian oscillation in these mice. Accordingly, PPARγ targets gain rhythmicity after acute HFD feeding [481]. Furthermore, the HFD-related hepatic fluctuations of PPARγ activity are driven by microbial communities in the gut as documented by antibiotic treatments that repress PPARγ-driven transcription in the liver. Therefore, HFD-induced remodeling in the gut microbiota mediates PPARγ-driven modulation of the host liver clock, resulting in adjusted circadian transcription [482]. . PPARα interacts directly with PER2 to modulate gene expression. In the muscle, PPARβ/δ regulates the expression of lipogenic genes in a rhythmic manner. PPARβ/δ is also an indirect target of the circadian clock via miR-122. PPARγ regulates expression of core circadian genes including Bmal1 and Rev-erbα, which support adipogenesis. In the liver, PPARγ also mediates the action of circadian triggers derived from gut microbiota in response to high-fat diet (HFD). PPARs bind to PPRE as PPAR:RXR heterodimers. Abbreviations: Acc-acetyl-CoA carboxylase; Fas-fatty acid synthase; Hmg-CoAR-β-hydroxy βmethylglutaryl-CoA reductase; PGC-1α-peroxisome proliferator-activated receptor γ coactivator 1 α; PPRE-PPAR response element; RXR-retinoid X receptor; Scd-1-stearoyl-CoA desaturase-1; Srebp-sterol regulatory element-binding protein 1.

Modulation of the Oxidative Status
CR exerts major beneficial effects through reducing ROS levels [483][484][485] in three distinct ways: (i) reduction of oxygen free-radical production by slowing metabolism, (ii) increasing rate of ROS neutralization, and (iii) accelerating the repair of ROS-damaged molecules [486][487][488][489][490]. In vivo, concentrations of ROS and several products of oxidation oscillate in a circadian manner in tissues and blood. The circadian clock is a major regulator of ROS homeostasis as proven by the fact that circadian protein mutants present increased levels of ROS and oxidative damage [491,492] and continuous light diminishes or abolishes the circadian rhythms of SOD, glutathione reductase (GR), and catalase (CAT) [493]. CAT activity peaks in humans at the beginning of the light phase in plasma [494], whereas in mice it culminates in the middle of the dark phase in the liver and kidneys [495] in accordance with eating patterns of these species. The antioxidant proteins peroxidoxins (PRX) show distinct daily fluctuation patterns in the SCN and the liver of Syrian hamsters [493] and, correspondingly, their day time expression changes are accompanied by differences in DNA damage, lipid peroxidation, and protein oxidation [493,[496][497][498][499]. In the rat cerebral cortex, SOD activity peaks in the dark phase, correlating with the highest level of malondialdehyde, which is build up in the process of lipid peroxidation [500]. In addition to the cerebellum, both Cu/Zn and Mn-SOD PPARβ/δ is also an indirect target of the circadian clock via miR-122. PPARγ regulates expression of core circadian genes including Bmal1 and Rev-erbα, which support adipogenesis. In the liver, PPARγ also mediates the action of circadian triggers derived from gut microbiota in response to high-fat diet (HFD). PPARs bind to PPRE as PPAR:RXR heterodimers. Abbreviations: Acc-acetyl-CoA carboxylase; Fas-fatty acid synthase; Hmg-CoAR-β-hydroxy β-methylglutaryl-CoA reductase; PGC-1α-peroxisome proliferator-activated receptor γ coactivator 1 α; PPRE-PPAR response element; RXR-retinoid X receptor; Scd-1-stearoyl-CoA desaturase-1; Srebp-sterol regulatory element-binding protein 1.

Modulation of the Oxidative Status
CR exerts major beneficial effects through reducing ROS levels [483][484][485] in three distinct ways: (i) reduction of oxygen free-radical production by slowing metabolism, (ii) increasing rate of ROS neutralization, and (iii) accelerating the repair of ROS-damaged molecules [486][487][488][489][490]. In vivo, concentrations of ROS and several products of oxidation oscillate in a circadian manner in tissues and blood. The circadian clock is a major regulator of ROS homeostasis as proven by the fact that circadian protein mutants present increased levels of ROS and oxidative damage [491,492] and continuous light diminishes or abolishes the circadian rhythms of SOD, glutathione reductase (GR), and catalase (CAT) [493]. CAT activity peaks in humans at the beginning of the light phase in plasma [494], whereas in mice it culminates in the middle of the dark phase in the liver and kidneys [495] in accordance with eating patterns of these species. The antioxidant proteins peroxidoxins (PRX) show distinct daily fluctuation patterns in the SCN and the liver of Syrian hamsters [493] and, correspondingly, their day time expression changes are accompanied by differences in DNA damage, lipid peroxidation, and protein oxidation [493,[496][497][498][499]. In the rat cerebral cortex, SOD activity peaks in the dark phase, correlating with the highest level of malondialdehyde, which is build up in the process of lipid peroxidation [500]. In addition to the cerebellum, both Cu/Zn and Mn-SOD expression levels show diurnal rhythmicity in the rat intestine and lung [501]. These fluctuations may depend on Per2 since Per2 KO mice have a lower amplitude of oscillations in hepatic Cu/Zn SOD expression and activity [502]. Along a similar line, KO of both Per1 and Per2 results in a 4h shift in the peak of Cu/Zn SOD expression, when compared to WT mice [502].
Reduced glutathione (GSH), in contrast to oxidized GSH (GSSG), is one of the most important non-enzymatic antioxidants. GSH acts as a scavenger of free radicals, supports the reduction of H 2 O 2 , and takes part in detoxification. Circadian oscillations have been demonstrated for GSH and the expression of enzymes necessary for the functional GSH system, including those involved in GSH reduction (GSH reductase (GR)], oxidation of GSH by reduction of H 2 O 2 [GSH peroxidase (GPx)], and conjugation of GSH [GSH S-transferase (GST)] to various electrophilic substrates [499,[503][504][505][506][507][508]. The reduction of GSSG to GSH is necessary to maintain the cellular redox status. This process involves NADP + and its redox partner NADPH, which were mentioned previously as they are inseparably connected with SIRTs. Moreover, in cultured rat fibroblasts high NAD + and NADP + levels decrease the binding capacity of CLOCK:BMAL1 heterodimers to E-box targets, whereas high levels of the reduced NADH and NADPH strongly enhances their binding in concert with BMAL1 acetylation, leading to increased expression of Pers, Crys, and other circadian genes [202]. Additionally, SIRT1 is also connected with another important marker of the circadian rhythm, melatonin. Melatonin acts as a synchronizer in mammals and provides temporal feedback to oscillators within the SCN and has been proven to display antioxidant properties [509]. Moreover, melatonin inhibits the expression and activity of SIRT1 [510]. Therefore, there is a complex connection between CR and circadian rhythm involving the redox status, SIRT1, and melatonin, at the border of oxidative and circadian systems.
PPARγ also regulates the expression of several antioxidant and pro-oxidant enzymes, as well as oxidative stress-related proteins, including GPx3 [513], CAT [514,515], and MnSOD [516]. Therefore, heart-specific PPARγ KO mice exhibit extensive oxidative damage accompanied by lower levels of MnSOD and elevated O 2 − levels in cardiac muscle [516]. Furthermore, PPARγ reduces the expression of inducible nitrogen monoxide (NO) synthase (iNOS) and stimulates endothelial NO synthase (eNOS) [517][518][519][520][521]. Conversely, the aorta of endothelial-specific PPARγ KO mice releases less NO than in WT animals, leading to an increase in oxidative stress [519]. Further, activation of PPARγ upregulates UCP2 expression in rats [522], which prevents O 2 − accumulation in the mitochondria and aids in the export of ROS from mitochondrial to the cytosol [523]. Moreover, CD36, the main target gene of PPARγ, supports the recognition and internalization of oxidized lipids [524][525][526]. Finally, PPARγ also increases Bcl-2 levels and thus, protects cardiomyocytes and glial cells from apoptosis triggered by oxidative stress [527,528]. Nuclear factor erythroid 2-related factor 2 (NRF2), a redox-sensitive transcriptional factor that serves as the master regulator of the oxidative stress response, cryoprotection against oxidative as well as electrophilic stress and takes part in inflammation suppression [529] contains PPREs in its gene promoter [530,531]. Reciprocally, NRF2 also binds the upstream promoter region and induces PPARγ [530,532] creating a positive feedback loop between PPARγ and NRF2. Interestingly, BMAL1 directly controls the expression of NRF2 [533], Hence, there is an overlap between the ability of PPARs to control oxidative stress and the effects of CR and circadian rhythm ( Figure 4).

Conclusions
Importantly, not only feeding schedule or feeding restriction (CR) but also nutrients entrain the circadian rhythm. A balanced combination of carbohydrate and protein induces phase advance in the liver clock, whereas the application of a single macronutrient (protein, carbohydrate, or fat) had no significant impact [634]. Similarly, the liver clock can be reset by the ingestion of a mix of glucose and amino acids, but not by either of the nutrients alone [26]. Therefore, various types of diet-related cues trigger an adjustment of circadian oscillations to adapt to the current metabolic status. In the case of CR for example, tissues need to optimize and synchronize their metabolic activity to reach beneficial homeostasis. The circadian clock controls the expression and activity of many rate-limiting enzymes and fine-tunes mechanisms of metabolic optimization and energy homeostasis. Because of its capacity to enable anticipation of daily events, it is superior in directing efficient use of energy. Resetting the circadian clocks through light, feeding, or pharmacological intervention grants metabolic health and rejuvenation underscoring the importance of the interplay between diets and biological clocks to promote longevity. Targeting molecular executors at the crossroad of CR and the circadian clock, like PPARs, may be an efficient and promising way to take advantage of the benefits of a well-calibrated circadian energy metabolism.   Figure 4. PPARs contribute to the processes regulated by CR and circadian rhythm at the whole-body level. CR exerts its beneficial effects by reducing oxidative stress, delaying aging, and inhibiting inflammation. Circadian rhythm affects all of these processes. PPARs regulate the expression of antioxidative enzymes, inflammatory cytokines, and metabolic processes. For the effects of SIRT refer to Figure 2. Abbreviations: AP-1-activating protein-1; CAT-catalase; COX-2-cyclooxygenase-2; eNOS-endothelial NO synthase; GPx-GSH peroxidase; GR-GSH reductase; GSH-glutathione; GSSG-oxidized glutathione; GST-GSH S-transferase; iNOS-inducible NO synthase; NRF2-nuclear factor erythroid 2-related factor 2; PRX-peroxiredoxins; ROS-reactive oxygen species; SOD-superoxide dismutase; STAT1-signal transducer and activator of transcription 1.

Reduction of Inflammation
The immune system as one of the key factors indispensable for survival has been intensively investigated in the context of circadian rhythms. The expression levels of toll-like receptors (TLRs) display significant circadian fluctuations in the mouse jejunum [534]. In the spleen and natural killer cells of rats, transcription of interferon γ (Ifnγ), granzyme B (GrB), perforin (Prf ), and Tnfα oscillate showing the highest expression at the break of the dark and the light phase which coincides with the cytolytic activity of splenic natural killer cells [535][536][537]. Similarly, the migration of lymphocytes through lymph nodes is regulated by circadian rhythm and peaks at the beginning of the active phase in mice [538]. Accordingly, the timing of triggering and generating immune response is decisive in the regulation of the efficiency of the reaction. After infection of the mouse gastrointestinal tract with Salmonella enterica serovar Typhimurium there is a more efficient bacteria clearance for infection done 4 h after the beginning of the dark phase compared to 4h into the light phase. Moreover, levels of antimicrobial peptide lipocalin-2 in the intestine are higher during the day than during the night [539]. Importantly, PPARγ and lipocalin-2 cooperate in response to bacterial infection in the intestine [540]. In the case of sepsis, the timing of the disease induction impacts the severity of the disease and coincides with the oscillations in TLR9 expression [541].
The central pacemaker can also regulate oscillations of inflammatory factors in peripheral organs as local splenic sympathectomy alters daily variations of cytokines, TNFα, and cytolytic factors, GRB, and PRF, in natural killer cells and splenocytes [542]. A lesion in the SCN leads to loss of daily variations in serum histamine, MCP-1 (CCL2), and IL-6 levels in mice [543] and dysregulated immune response with higher levels of cytokines after administration of lipopolysaccharide (LPS) in rats [544]. Moreover, night administered LPS increases SCN basal neuronal activity [544] and induces more severe responses in body temperature and cytokine production than LPS given in the rest period [544]. However, the inflammatory response of the mouse lung to LPS is strongest during the day compared to the night [545]. Finally, rodents with a reversed feeding schedule, consuming during the light phase, and fasting at night, show inadequate inflammatory response to LPS in association with elevated TNFα and IL-6 levels and increased mortality [546,547].
Clock genes have been shown to modulate the expression of multiple inflammatory cytokines. CRY affects TNFα, IL1β, and Il-6 [548], RORα impacts Il-1β, and Il-6 [548], PER2 modulates daily rhythms of IFNγ [549], and Il-1β [550], Rev-ERBα regulates IL-6 [551], MCP-1 as well as TH17 cells [552], while Bmal1 regulates diurnal oscillation of Ly6C hi inflammatory monocytes [553] and reduces monocyte recruitment [554]. Consequently, Clock KO mice exhibit reduced cytokine production in macrophages in response to the LPS challenge or Salmonella Typhimurium infection [539]. On the contrary, deletion of the negative clock arm Ror-α in mice leads to a stronger LPS-induced inflammation with higher levels of Il-1β, IL-6, and macrophage inflammatory protein-2 in bronchoalveolar lavage fluids from mice lungs [548]. Moreover, T and B cell development is defective in these mice while mast cells and macrophages produce an increased amount of TNFα and IL-6 upon activation [555]. Per2 KO mice are more resistant to the LPS challenge compared to WT mice [550]. KO or silencing of Cry1 and Cry2 also leads to increased inflammation, a permanent elevation of proinflammatory cytokines in a cell-autonomous manner, and constitutive activation of NF-κB and PKA signaling [556]. Importantly, CLOCK is required for acetylation of p65, a key event for NF-κB transactivation and downstream cytokine production [557]. NF-κB is a transcriptional regulator of major importance and its action is usually pro-inflammatory and pro-oxidant. NF-κB induces the expression of the inflammatory cytokines sIL-1β, IL-6, and TNFα, as well as the proinflammatory enzymes cyclooxygenase-2 (COX-2) and iNOS. However, it can also affect the expression of Sod and other anti-inflammatory genes [558][559][560][561][562][563]. CR exhibits a broad anti-inflammatory effect and it acts, among others, through NF-κB and IκB to blunt age-triggered increases in COX-2 levels and activity. CR suppresses the generation of iNOS, IL-β, IL-6, TNFα, and prostanoids, such as TXA 2, prostacyclin 2, and prostaglandin E2 [59,485] while dampening aging-related reduction of PPAR expression and activity [229]. PPARs interfere with several pro-inflammatory mediators including NF-κB, signal transducer and activator of transcription 1 (STAT1), and activating protein-1 (AP-1) [564][565][566][567] inhibiting the expression of genes coding for inflammatory proteins, such as COX-2, iNOS, cytokines, metalloproteases, and acute-phase proteins [520,565]. Moreover, inflammatory eicosanoids including prostaglandins and leukotrienes act as ligands for PPARs [568,569]. However, each PPAR exhibits a set of distinct anti-inflammatory activities [569].
The anti-inflammatory properties of PPARα are mostly based on its interaction with NF-κB. Accordingly, PPARα KO results in premature oxidative stress and an aggravated inflammatory response as well as a stronger age-dependent increase in oxidative stress and NF-κB activity [226,570]. In agreement with these observations, agonists of PPARα reduce the activity of NF-κB and the spontaneous generation of inflammatory cytokines [226,571]. Importantly, high doses of PPARα ligands induce NF-κB, whereas low amounts reduce NF-κB activation [226]. PPARα contributes to the anti-inflammatory properties of CR by reducing the expression of acute-phase genes (C4bp, C9, Mbl1, Orm1, and Saa4) responsive to inflammatory cytokines [232].

Prevention of Aging
Circadian clocks have been implicated in the control of aging and aging-associated pathologies via regulation of oxidative stress, cell cycle, cell death, and DNA damage response [597]. Importantly, aging is accompanied by a shift in the phase and a decrease in amplitude of rhythmic oscillations [598][599][600][601]. In mice, age-dependent degradation of neuronal activity rhythms in the SCN results in the disruption of the physiological ability to entrain to external light cues, and approximately 20 times reduced sensitivity to the synchronizing effect of light compared to young animals; this leads to an alteration of rhythmic behavior [602][603][604][605][606]. Circadian rhythm-related changes during aging include body temperature, activity-wakefulness, locomotor activity patterns, and drinking behavior. In humans, aging is linked with a phase advance in the rhythms of body temperature and melatonin secretion as well as the earlier habitual time of sleeping and disturbed sleep [607,608]. Further, age alters the 24 h expression profile of Clock and Bmal1 in the hamster SCN [609] and results in alterations in the circadian transcriptome. The decline occurs in a tissue-specific manner, as documented by the comparison of transcriptional patterns in the liver versus epidermal and skeletal muscle stem cells [70].
Bmal1 KO mice have reduced lifespans and display various symptoms of premature aging including sarcopenia, cataracts, lesser subcutaneous fat, and organ shrinkage [13]. Deficiency of CLOCK also impacts longevity with the average and maximum lifespan of Clock KO mice being reduced by 15% and 20%, respectively, compared to WT mice. This phenotype is accompanied by a high rate of development of age-related pathologies, cataracts, and dermatitis [11]. However, the mice do not develop the premature aging phenotype that characterizes Bmal1 KO mice. Based on wheel-running activity, the circadian rhythm in Per1, 2 KO mice is out of control. These animals also display signs of premature aging including loss of soft tissues and kyphosis, a more rapid decline in fertility and litter size in each successive breeding, as well as a significant increase in neoplastic and hyperplastic phenotypes [610]. Per1 and Per2 were proposed as longevity-associated candidate genes based on (i) the KO phenotype in mice, (ii) comparison of the activity of a group of genes with differential expression in several tissues such as the liver, brain, skeletal muscles of mice subjected to CR and (iii) Per gene expression in the long-lived dwarf mice [610,611]. In golden hamsters carrying a 20 h-period mutation, tau, resulting in 24 h light-dark cycles, life span is shortened. However, in aged animals, longevity can be extended by fetal suprachiasmatic implants which restore higher amplitude rhythms [612][613][614].
Oscillation disturbance by weekly reversal of the light-dark cycle reduces the survival time of cardiomyopathic hamsters [615]. Transplanting fetal SCN to an old hamster with weak behavioral rhythms restores robust rhythmicity in the transplanted hosts [616], triggers a greater response to the phase-shifting effects of triazolam [617], and increases lifespan by 4 months [612]. In humans, a disrupted circadian rhythm in night-shift workers leads to metabolic disorders, increased incidence of cancer, hormone imbalance, gastrointestinal abnormalities, cardiovascular diseases, reproductive aberrations, sleep and psychological disorders including depression and anxiety [618].
In young mice, 2626 liver genes present a rhythmic expression, while in old mice this number is reduced to 1664 genes, including 1575 genes that are rhythmic in both groups and comprise the core clock genes. CR increases the number and amplitude of rhythmic genes in both young and old mouse livers [70]. Besides gene expression, also global protein acetylation is highly circadian. The acetylation rhythm is dampened in aged mice and can be rescued by CR [70]. Moreover, in aging epidermal stem cells (epSCs) and skeletal muscle stem cells (muSCs), CR maintains or restores rhythmic homeostatic functions [619]. As in mice, CR in Drosophila enhances the amplitude of cycling of most circadian clock genes, including Tim, in peripheral tissues [480]. Per and Tim KOs attenuate the CR-dependent changes in lifespan extension and fat metabolism [480]. Similarly, 30% CR does not increase the lifespan or have an impact on the physiology of Bmal1 KO mice [71]. Therefore, some of the circadian clock genes are necessary for the full benefits of CR.
Aging is associated with decreased metabolic rate, impaired mitochondrial function, reduced insulin resistance, dysfunctional lipid metabolism, immunosenescence, increased oxidative stress, and lowered hormonal secretion [620][621][622]. Glucose metabolism and insulin sensitivity maintenance is a key feature of the anti-aging actions of CR [54,75]. In fact, genes connected with the insulin/IGF-1 signaling pathway, which include PPARs, have been proposed as longevity candidate markers [77,78,623]. Similarly, healthy adipose tissue has also been directly associated with lifespan due to its role in energy metabolism [74,391], which strongly relies on PPARγ [624][625][626]. Therefore, in gene network analysis, PPARγ was identified as one of the "longevity genes" in mouse WAT [627].
Most of the multiple traits of aging are related to PPARs and mTOR activity, oxidative stress, inflammation, and metabolism ( Figure 4). Furthermore, aging-associated changes in PPAR expression and activity are reversed by CR [229,268,628,629]. The impact of PPARs can be particularly well observed in mutant models of longevity, the dwarf mice, a model that shows multiple similarities with CR animals [630]. These mice are characterized by a hepatic increase in PPARα expression and constitutive activation of some of its target genes including genes involved in βand ω-oxidation of FAs [220,631]. Growth hormone receptor (GHR) KO dwarf mice show similar expression patterns of PPARα in the muscle and PPARβ/δ in the liver and skeletal muscle as WT CR mice [225]. Fittingly, mice overexpressing the bovine GH gene, which are characterized by short life span, exhibit a decrease in hepatic expression of PPARα and genes involved in FA activation, β-oxidation as well as the production of ketone bodies leading to limited capacity to adjust to fasting [632]. Furthermore, overexpression of FGF21, a PPARα target gene, extends lifespan in mice and blunts GH/IGF-1 signaling in the liver [633]. Therefore, the resetting of circadian rhythms by CR can lead to increased longevity and PPARs play a vital role in the rejuvenating outcomes of CR.

Conclusions
Importantly, not only feeding schedule or feeding restriction (CR) but also nutrients entrain the circadian rhythm. A balanced combination of carbohydrate and protein induces phase advance in the liver clock, whereas the application of a single macronutrient (protein, carbohydrate, or fat) had no significant impact [634]. Similarly, the liver clock can be reset by the ingestion of a mix of glucose and amino acids, but not by either of the nutrients alone [26]. Therefore, various types of diet-related cues trigger an adjustment of circadian oscillations to adapt to the current metabolic status. In the case of CR for example, tissues need to optimize and synchronize their metabolic activity to reach beneficial homeostasis. The circadian clock controls the expression and activity of many rate-limiting enzymes and fine-tunes mechanisms of metabolic optimization and energy homeostasis. Because of its capacity to enable anticipation of daily events, it is superior in directing efficient use of energy. Resetting the circadian clocks through light, feeding, or pharmacological intervention grants metabolic health and rejuvenation underscoring the importance of the interplay between diets and biological clocks to promote longevity. Targeting molecular executors at the crossroad of CR and the circadian clock, like PPARs, may be an efficient and promising way to take advantage of the benefits of a well-calibrated circadian energy metabolism.
Author Contributions: Writing-original draft preparation and visualization K.D.; writing-review and editing, W.W. Both authors have read and agreed to the published version of the manuscript.
Funding: The writing of this review received no external funding.

Acknowledgments:
The authors would like to acknowledge András Gregor for his help in correcting the manuscript. Open Access Funding by the University of Vienna.

Conflicts of Interest:
The authors declare no conflict of interest.