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Open AccessArticle

Alterations in IL-6/STAT3 Signaling by Korean Mistletoe Lectin Regulate the Self-Renewal Activity of Placenta-Derived Mesenchymal Stem Cells

1
Department of Food and Nutrition, Kyungnam University, Gyeongsangnam-do 51767, Korea
2
Department of Oral Pathology, College of Dentistry, Gangneung-Wonju National University, Gangneung-si 25457, Korea
3
Department of Biomedical Science, CHA University, Seongnam-si 13488, Korea
4
Department of Pathology, Korea University College of Medicine, Seoul 02841, Korea
*
Author to whom correspondence should be addressed.
These authors (G.D.K. and J.H.C.) contributed equally to this work.
Nutrients 2019, 11(11), 2604; https://doi.org/10.3390/nu11112604
Received: 24 September 2019 / Revised: 19 October 2019 / Accepted: 25 October 2019 / Published: 30 October 2019
Korean mistletoe (Viscum album L. var. coloratum) lectin (VCA) is known as an anticancer drug. However, it is not clear whether VCA affects the self-renewal activity of mesenchymal stem cells (MSCs). Therefore, the objectives of this study were to analyze the effect of VCA on the proliferation of MSCs and expression of stemness markers. We also evaluated the usefulness of placenta-derived MSCs (PD-MSCs) as a screening tool. VCA was stably administered to MSCs, and analyzed self-renewal activities. The effect of IL-6 signaling on MSC proliferation was explored by quantitative methylation-specific PCR (qMSP) and western blot analysis. Compared with the control condition, low concentrations of VCA (10 pg/mL) induced an increase in the self-renewal activity of MSCs. Interestingly, a low concentration of VCA promoted IL-6 signaling in PD-MSCs through altered IL-6/STAT3 gene methylation. Furthermore, inhibition of IL-6 expression in PD-MSCs using an anti-IL-6 antibody caused a decrease in their self-renewal activity through IL-6/STAT3 signaling by altering IL-6/STAT3 gene methylation. These findings provide helpful data for understanding the mechanism of MSC self-renewal via VCA and show that VCA may be useful as a functional natural product for developing efficient therapies using placenta-derived stem cells. View Full-Text
Keywords: mesenchymal stem cell; mistletoe lectin; VCA; self-renewal; IL-6; methylation mesenchymal stem cell; mistletoe lectin; VCA; self-renewal; IL-6; methylation
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Kim, G.D.; Choi, J.H.; Lim, S.M.; Jun, J.H.; Moon, J.W.; Kim, G.J. Alterations in IL-6/STAT3 Signaling by Korean Mistletoe Lectin Regulate the Self-Renewal Activity of Placenta-Derived Mesenchymal Stem Cells. Nutrients 2019, 11, 2604.

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