Effects of Obesity and Gastric Bypass Surgery on Nutrient Sensors, Endocrine Cells, and Mucosal Innervation of the Mouse Colon
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. RYGB Surgery
2.3. Experimental Overview
2.4. Gene Expression Studies
2.5. Immunohistochemistry
2.6. Ussing Chamber Experiments
2.7. Nutrient Solutions
2.8. Statistical Analysis
3. Results
3.1. Obesity-Induced Up-Regulation of Colonic Nutrient-Sensing Receptors
3.2. RYGB Further Up-Regulates Expression of Specific Nutrient-Sensing Receptors
3.3. mRNA Expression of Appetite Modulation Hormones/Peptides Increases in Obesity and RYGB Both Increase Colonic L-Cell and EC Cell Numbers
3.4. Mucosal Innervation Is Unaltered by Diet and RYGB
3.5. Mucosal Stimulation with Specific Nutrients Activates Colonocytes
4. Discussion
Author Contributions
Funding
Conflicts of Interest
References
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Peiris, M.; Aktar, R.; Raynel, S.; Hao, Z.; Mumphrey, M.B.; Berthoud, H.-R.; Blackshaw, L.A. Effects of Obesity and Gastric Bypass Surgery on Nutrient Sensors, Endocrine Cells, and Mucosal Innervation of the Mouse Colon. Nutrients 2018, 10, 1529. https://doi.org/10.3390/nu10101529
Peiris M, Aktar R, Raynel S, Hao Z, Mumphrey MB, Berthoud H-R, Blackshaw LA. Effects of Obesity and Gastric Bypass Surgery on Nutrient Sensors, Endocrine Cells, and Mucosal Innervation of the Mouse Colon. Nutrients. 2018; 10(10):1529. https://doi.org/10.3390/nu10101529
Chicago/Turabian StylePeiris, Madusha, Rubina Aktar, Sarah Raynel, Zheng Hao, Michael B. Mumphrey, Hans-Rudolf Berthoud, and L. Ashley Blackshaw. 2018. "Effects of Obesity and Gastric Bypass Surgery on Nutrient Sensors, Endocrine Cells, and Mucosal Innervation of the Mouse Colon" Nutrients 10, no. 10: 1529. https://doi.org/10.3390/nu10101529