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Correction published on 8 March 2010, see Nutrients 2010, 2(3), 317-318.
Open AccessArticle

Whey Protein Ingestion Activates mTOR-dependent Signalling after Resistance Exercise in Young Men: A Double-Blinded Randomized Controlled Trial

Molecular Nutrition Unit, Deakin University, Burwood, Victoria, 3125, Australia
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Nutrients 2009, 1(2), 263-275; https://doi.org/10.3390/nu1020263
Received: 10 November 2009 / Accepted: 4 December 2009 / Published: 14 December 2009
The effect of resistance exercise with the ingestion of supplementary protein on the activation of the mTOR cascade, in human skeletal muscle has not been fully elucidated. In this study, the impact of a single bout of resistance exercise, immediately followed by a single dose of whey protein isolate (WPI) or placebo supplement, on the activation of mTOR signalling was analyzed. Young untrained men completed a maximal single-legged knee extension exercise bout and were randomized to ingest either WPI supplement (n = 7) or the placebo (n = 7). Muscle biopsies were taken from the vastus lateralis before, and 2, 4 and 24 hr post-exercise. WPI or placebo ingestion consumed immediately post-exercise had no impact on the phosphorylation of Akt (Ser473). However, WPI significantly enhanced phosphorylation of mTOR (Ser2448), 4E-BP1 (Thr37/46) and p70S6K (Thr389) at 2 hr post-exercise. This study demonstrates that a single dose of WPI, when consumed in modest quantities, taken immediately after resistance exercise elicits an acute and transient activation of translation initiation within the exercised skeletal muscle. View Full-Text
Keywords: leucine; BCAA; p70S6K; 4E-BP1; resistance exercise leucine; BCAA; p70S6K; 4E-BP1; resistance exercise
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Farnfield, M.M.; Carey, K.A.; Gran, P.; Trenerry, M.K.; Cameron-Smith, D. Whey Protein Ingestion Activates mTOR-dependent Signalling after Resistance Exercise in Young Men: A Double-Blinded Randomized Controlled Trial. Nutrients 2009, 1, 263-275.

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