Optical Backscattering Measured by Airborne Lidar and Underwater Glider

The optical backscattering from particles in the ocean is an important quantity that has been measured by remote sensing techniques and in situ instruments. In this paper, we compare estimates of this quantity from airborne lidar with those from an in situ instrument on an underwater glider. Both of these technologies allow much denser sampling of backscatter profiles than traditional ship surveys. We found a moderate correlation (R = 0.28, p < 10−5), with differences that are partially explained by spatial and temporal sampling mismatches, variability in particle composition, and lidar retrieval errors. The data suggest that there are two different regimes with different scattering properties. For backscattering coefficients below about 0.001 m−1, the lidar values were generally greater than the glider values. For larger values, the lidar was generally lower than the glider. Overall, the results are promising and suggest that airborne lidar and gliders provide comparable and complementary information on optical particulate backscattering.


Introduction
The spectral characteristics of remote sensing reflectance (R rs ) measured from space have provided a global view of the upper ocean.Remote sensing reflectance is a function of both the apparent and inherent optical properties of water (AOPs and IOPs), the latter of which includes the particulate backscattering coefficient (b bp ), the backscattering coefficient of seawater (b bw ), and several absorption terms [1][2][3].Therefore, accurate measurements of b bp are necessary to better understand remote sensing measurements.The use of b bp in expressions for remote sensing reflectance is an approximation, since any ocean color measurement from space is made at a single scattering angle that depends on the solar zenith angle.The approximation is justified by calculations showing an error <10% for a "wide range of scattering phase functions" for solar zenith angles >20 • [3].In more targeted studies, b bp has been related to the total particulate material [4,5], particulate organic carbon [6,7], and the carbon content of phytoplankton [8][9][10].
In situ measurement of b bp is facilitated by the fact that, because the variability in the shape of the particulate volume scattering function at scattering angles >90 • is relatively low [11][12][13][14][15][16][17], a relationship between the particulate backscattering coefficient and volume scattering functions that can be expressed as [12][13][14][15][16][17] b bp = 2π π π 2 β p (θ) sin(θ)dθ = 2π χ(θ 0 )β p (θ 0 ), (1) where β p (θ) is the particulate volume scattering function at scattering angle θ and χ is a conversion factor defined by this equation.Direct measurement of backscattering at all angles >90 • is difficult, but this relationship allows us to estimate b bp from measurement of β p at a single angle, denoted by θ 0 , [18] with an error around 10% [19].This can be improved to about 3% using measurements at two scattering angles [20] and to under 1% with a geometry that provides the integrated scattering with a diffuse cavity [19].
Compact instruments that measure optical scattering at a single angle with source-receiver separations of a few cm can be deployed on underwater gliders.Gliders are small, autonomous underwater vehicles that manipulate their buoyancy to continuously profile the water column [22] while "gliding" horizontally, collecting thousands of measurements during deployments typically ranging between a few days and a few months.Because of their unique propulsion mechanism, gliders allow energy and cost-efficient measurements on spatial and temporal scales that are difficult to make by other means [23].Gliders outfitted with fluorometers are routinely used for monitoring the phytoplankton community structure of the West Florida Shelf [24][25][26][27], where they have been particularly useful in describing the 3-dimensional structure of blooms of Karenia brevis, the Florida red-tide organism.
Profiles of optical scattering have been made by airborne lidar [28].This technique has been particularly effective in measuring enhanced scattering layers in the ocean [29,30] and dynamical processes such as internal waves [31,32].A direct comparison of airborne lidar estimates of b bp with ship based measurements produced a root-mean square difference of 9.4 × 10 −4 m −1 over a range of values from 5 × 10 −4 m −1 to 9 × 10 −3 m −1 [33].This agreement was obtained using χ(180 • ) = 0.5.
In this work, we describe results from a field campaign in which airborne lidar measurements were obtained simultaneous with glider measurements along a routine harmful algae bloom monitoring transect on the West Florida Shelf.The primary objective of this paper is to compare estimates of b bp from airborne lidar with those from an in situ scatterometer on a glider to determine if these measurements are consistent with one another and can be quantitatively compared to produce a complementary data set.

Materials and Methods
The study area (Figure 1) comprised two transect lines on the West Florida Shelf between Tampa Bay and Charlotte Harbor.In the initial transect design, water depth varied from 20 m at the eastern end of the lines to 50 m at the western end.The northern transect was extended to the southwest past the original waypoint in response to a feature observed in satellite images.The aircraft tracks followed the original design, and this is the reason for the deviation between the glider track and the aircraft tracks in the figure.The Slocum G1 glider (Teledyne Webb Research) was deployed at 82.89°W longitude, 27.28°N latitude on 6 July 2015, reached the apex (83.43°W, 26.71°N) on 12 July and turned back towards the coast, and was finally retrieved on 17 July at 82.71°W, 26.98°N.During this period, it made a total of 3372 profiles (up or down) between 2 m below the surface and 2 m above the bottom (Slocum gliders constrain vertical travel using both pressure sensors and altimeters).An ECO-BBFL2 (WET Labs) "puck" in the central payload bay measured scattering of red (650 nm) light at a scattering angle of 124°.The optical backscatter data were processed as follows: first, the sensor calibration was applied to the data to obtain the volume scattering function, β(124°, 650 nm).The contribution from sea water was estimated as (2) where the reference value βw(90°, 600 nm) = 8.8 × 10 −5 m −1 •sr −1 was taken from Morel [34].This was subtracted from β, leaving the particulate contribution, βp.This was then multiplied by 2πχ with χ = 1.08 (6.8 sr) to get bb(650 nm) [15].For each of the two transects, these data were averaged into bins of one meter in depth and 0.1° in longitude.Remote sensing data were collected with the NOAA Oceanographic Lidar on a NOAA Twin Otter aircraft operating at an altitude of 300 m.Both transect lines were flown on 8 July, 10 July, and 13 July.In addition, the northern line was flown on 15 July and on the early morning of 17 July.With slight modifications, the lidar is the same as has been described in previous publications [29,[35][36][37].For this application, the transmitted pulse energy was 100 mJ, pulse length was 12 ns, pulse repetition rate was 30 s −1 , wavelength was 532 nm, polarization was linear, and the beam divergence was 17 mrad.Two receiver telescopes were used to provide two lidar channels; one was unpolarized to collect all light regardless of polarization and the other was polarized to collect light cross-polarized with respect to the transmitted light.The receiver telescopes and transmitted beam were aligned to provide complete overlap at the surface.Each telescope had an aperture at the focus of the primary lens to limit the field of view of the receiver to the divergence of the transmitter, an interference filter (1 nm bandwidth) to limit background light, and a photomultiplier tube to convert the collected light into an electrical signal.For each signal, a logarithmic amplifier compressed the dynamic range of the signal and an 8-bit digitizer sampled and recorded the result at 1 GHz.The beam was not scanned, but was instead pointed at a fixed angle of 15° off nadir.During this period, it made a total of 3372 profiles (up or down) between 2 m below the surface and 2 m above the bottom (Slocum gliders constrain vertical travel using both pressure sensors and altimeters).An ECO-BBFL2 (WET Labs) "puck" in the central payload bay measured scattering of red (650 nm) light at a scattering angle of 124 • .The optical backscatter data were processed as follows: first, the sensor calibration was applied to the data to obtain the volume scattering function, β(124 • , 650 nm).The contribution from sea water was estimated as where the reference value β w (90 • , 600 nm) = 8.8 × 10 −5 m −1 •sr −1 was taken from Morel [34].This was subtracted from β, leaving the particulate contribution, β p .This was then multiplied by 2πχ with χ = 1.08 (6.8 sr) to get b b (650 nm) [15].For each of the two transects, these data were averaged into bins of one meter in depth and 0.1 • in longitude.Remote sensing data were collected with the NOAA Oceanographic Lidar on a NOAA Twin Otter aircraft operating at an altitude of 300 m.Both transect lines were flown on 8 July, 10 July, and 13 July.In addition, the northern line was flown on 15 July and on the early morning of 17 July.With slight modifications, the lidar is the same as has been described in previous publications [29,[35][36][37].For this application, the transmitted pulse energy was 100 mJ, pulse length was 12 ns, pulse repetition rate was 30 s −1 , wavelength was 532 nm, polarization was linear, and the beam divergence was 17 mrad.Two receiver telescopes were used to provide two lidar channels; one was unpolarized to collect all light regardless of polarization and the other was polarized to collect light cross-polarized with respect to the transmitted light.The receiver telescopes and transmitted beam were aligned to provide complete overlap at the surface.Each telescope had an aperture at the focus of the primary lens to limit the field of view of the receiver to the divergence of the transmitter, an interference filter (1 nm bandwidth) to limit background light, and a photomultiplier tube to convert the collected light into an electrical signal.For each signal, a logarithmic amplifier compressed the dynamic range of the signal and an 8-bit digitizer sampled and recorded the result at 1 GHz.The beam was not scanned, but was instead pointed at a fixed angle of 15 • off nadir.
Processing of the unpolarized lidar data involved several steps.For each pulse, strong surface return was identified, and the depth of subsequent samples were obtained using the speed of light in seawater.Profiles were averaged over 25 pulses (about 60 m along the flight track).Next, the logarithm of each of the averaged signal profiles was fit to a quadratic.
where S is the total lidar return, z is depth, and the c i are the fit coefficients.The top of the range for the fit was set at a depth of 5 m so that the surface reflection would not affect the results.The bottom of the range was taken to be the depth at which the signal was 60 dB below the surface return or 0.8 times the water depth, whichever was less for that particular profile.The reason for using a fit was to allow extrapolation to the surface and top smooth out small variations.The quadratic was chosen because it provided the best fit to the profiles overall.The lidar attenuation coefficient is then Assuming a constant lidar ratio [38], the volume backscatter was then estimated by where A is a calibration coefficient based on laboratory measurements.The contribution of water to the volume scattering function was estimated by 1.94 × 10 −4 m −1 •sr −1 [38] and subtracted from the total to obtain the particulate volume scattering function.This was converted into an estimate of b bp using a factor of 2π sr (χ = 1).This value for χ is within the range of reported measurements, and the results can easily be scaled to accommodate different values.The ramifications of this choice are considered in Section 4. The resulting data were binned into the same depth and longitude bins as the glider data.A slightly larger depth range was used for the comparison than was used for the fit-2 m to 60 dB or 0.9 times water depth.
To compare glider and lidar data, we calculated the Pearson correlation coefficient of the binned data for each transect of each flight.In scatter plots of these data, we noticed anomalously low values of lidar data, generally near the limit of the depth range.These values were included in the presentation of the binned backscatter, but the deepest bin at each position was removed before calculation of correlations.
The data from the cross-polarized channel were processed in much the same way as those from the unpolarized channel.The primary difference was that the contribution of water to the volume scattering function is zero for the cross-polarized return at a scattering angle of 180 • .Also, the calibration coefficient was different for this channel, because the channels are not identical.Since the cross-polarized channel provides an estimate of the cross-polarized component of the volume backscattering coefficient, β X (π), the final result of this process is not b bp but the product of depolarization, d, and b bp , where the depolarization is defined by The maximum possible value of d is 0.5, when the scattering is completely depolarizing.

Results
The binned glider data (Figure 2) show enhanced backscatter near the bottom on both transects.Both legs also show enhanced backscatter between 20 and 30 m depths in the western part of each transect.The binned lidar data (Figure 3) show similar features, but there are significant differences.The major features were similar in all flights; the figure shows the average of the first three flights, where we have data from both transects.The major difference is that the lidar signal only penetrates to about 30 m, and the enhancement near the bottom cannot be seen in the western part of the transects where the bottom is deeper than that.The other obvious difference is that the contrast between regions of strong scattering and general background levels is greater in the in situ data than in the lidar data.These data also clearly show the very low values near the bottom of the depth range.transects where the bottom is deeper than that.The other obvious difference is that the contrast between regions of strong scattering and general background levels is greater in the in situ data than in the lidar data.These data also clearly show the very low values near the bottom of the depth range.Significant correlations were obtained between the glider and the unpolarized lidar bbp data (Table 1), with an overall average of R = 0.28.Because of the large number of points in each correlation (1100 for the northern transect and 1600 for the longer southern transect), all were highly significant (p < 10 −5 ), except for two flights where the correlation for the cross-polarized return was not significantly correlated with the glider data.The average correlation for the northern transect was slightly greater than for the southern transect (0.32 vs. 0.21), consistent with the better spatial and temporal match between the northern transect flight and glider tracks.The best correlation was observed on the northern transect when the glider was closest to the center of the transect (10 July).The results for the cross-polarized lidar return were similar, with an overall average correlation of R = 0.45 between lidar and glider-measured bbp and higher correlation on the northern transect than on the southern (0.48 vs. 0.38).Significant correlations were obtained between the glider and the unpolarized lidar b bp data (Table 1), with an overall average of R = 0.28.Because of the large number of points in each correlation (1100 for the northern transect and 1600 for the longer southern transect), all were highly significant (p < 10 −5 ), except for two flights where the correlation for the cross-polarized return was not significantly correlated with the glider data.The average correlation for the northern transect was slightly greater than for the southern transect (0.32 vs. 0.21), consistent with the better spatial and temporal match between the northern transect flight and glider tracks.The best correlation was observed on the northern transect when the glider was closest to the center of the transect (10 July).The results for the cross-polarized lidar return were similar, with an overall average correlation of R = 0.45 between lidar and glider-measured b bp and higher correlation on the northern transect than on the southern (0.48 vs. 0.38).As an example of the scatter in the data, we present the data for the north transect on 10 July (Figure 4).The backscatter values (Figure 4a) are scattered around the 1:1 line, but the figure suggests that there are two different regimes.For values below about 0.001 m −1 , the lidar data are generally greater than the glider data.For larger values, the lidar data are generally lower than the glider data.When averaged over all flights, the lidar data were 71% higher than the glider data for the small values, but 30% lower for the large values.There was no difference when the north and south transects were treated separately.The root-mean square (rms) difference between lidar and glidermeasured bbp for this flight was 5.4 × 10 −4 m −1 .The average over all flights, 8.6 × 10 −4 m −1 , was slightly less than previous results [33].Removing the effects of the difference in mean values, we found the rms difference to be 73% of the average glider value for the low values and 50% for the high values.The short dashed line is the figure shows what the 1:1 line would be, relative to the data, had we used χ(180°) = 0.5.Most of the low values are between these two lines, while most of the high values fall below the 1:1 line obtained with χ(180°) = 1, but above the long dashed lined obtained with χ(180°) =  As an example of the scatter in the data, we present the data for the north transect on 10 July (Figure 4).The backscatter values (Figure 4a) are scattered around the 1:1 line, but the figure suggests that there are two different regimes.For values below about 0.001 m −1 , the lidar data are generally greater than the glider data.For larger values, the lidar data are generally lower than the glider data.When averaged over all flights, the lidar data were 71% higher than the glider data for the small values, but 30% lower for the large values.There was no difference when the north and south transects were treated separately.The root-mean square (rms) difference between lidar and glider-measured b bp for this flight was 5.4 × 10 −4 m −1 .The average over all flights, 8.6 × 10 −4 m −1 , was slightly less than previous results [33].Removing the effects of the difference in mean values, we found the rms difference to be 73% of the average glider value for the low values and 50% for the high values.The short dashed line is the figure shows what the 1:1 line would be, relative to the data, had we used χ(180 • ) = 0.5.Most of the low values are between these two lines, while most of the high values fall below the 1:1 line obtained with χ(180 • ) = 1, but above the long dashed lined obtained with χ(180 • ) = 2.There is also evidence of the two regimes in the lidar attenuation values (Figure 4b), where the attenuation at the higher backscatter values is less than might be expected from an extrapolation of the trend in the lower values.
Remote Sens. 2017, 9, 379 7 of 12 2. There is also evidence of the two regimes in the lidar attenuation values (Figure 4b), where the attenuation at the higher backscatter values is less than might be expected from an extrapolation of the trend in the lower values.As expected, there was some variability from flight to flight.The correlation between flights is plotted in Figure 5 as a function of the number of days between the flights.For the northern transect, there were three pairs of flights separated by 2 days and two separated by 5 days, and the numbers are averages of these values.The other points represent single pairs of flights.Overall, the correlation at a 2 day separation is similar to the correlation between the lidar and the glider-significant, but not very high.This correlation decreases with increasing delay.As expected, there was some variability from flight to flight.The correlation between flights is plotted in Figure 5 as a function of the number of days between the flights.For the northern transect, there were three pairs of flights separated by 2 days and two separated by 5 days, and the numbers are averages of these values.The other points represent single pairs of flights.Overall, the correlation at a 2 day separation is similar to the correlation between the lidar and the glider-significant, but not very high.This correlation decreases with increasing delay.
Remote Sens. 2017, 9, 379 7 of 12 2. There is also evidence of the two regimes in the lidar attenuation values (Figure 4b), where the attenuation at the higher backscatter values is less than might be expected from an extrapolation of the trend in the lower values.As expected, there was some variability from flight to flight.The correlation between flights is plotted in Figure 5 as a function of the number of days between the flights.For the northern transect, there were three pairs of flights separated by 2 days and two separated by 5 days, and the numbers are averages of these values.The other points represent single pairs of flights.Overall, the correlation at a 2 day separation is similar to the correlation between the lidar and the glider-significant, but not very high.This correlation decreases with increasing delay.The results for the cross-polarized return, which include the effects of both d and b bp , (Figure 6) show similar patterns to the unpolarized return lidar signal.The contrast between the scattering layers and background levels is greater, however, consistent with previous observations [30,31].The scattering from pure sea water of 650 nm light at 124 • is less than that of 532 nm light at 180 • by a factor of 0.29, so the contrast from the scatterometer will be higher than that from the unpolarized lidar return.There is no cross-polarized lidar return from pure sea water, so the contrast is also higher than that from the unpolarized lidar return.
The results for the cross-polarized return, which include the effects of both d and bbp, (Figure 6) show similar patterns to the unpolarized return lidar signal.The contrast between the scattering layers and background levels is greater, however, consistent with previous observations [30,31].The scattering from pure sea water of 650 nm light at 124° is less than that of 532 nm light at 180° by a factor of 0.29, so the contrast from the scatterometer will be higher than that from the unpolarized lidar return.There is no cross-polarized lidar return from pure sea water, so the contrast is also higher than that from the unpolarized lidar return.

Discussion
This work highlights similarities and differences in the measurement of bbp by airborne lidar and glider-based in situ instruments.Both use a measurement of scattering at a single angle to infer bbp.This probably works better for the in situ scattering angle near 120°, because there is less variability in the relationship between bbp and scattering at this angle than there is at the lidar scattering angle of 180° [12][13][14][15][16][17].Both provide much greater sampling density than traditional ship surveys.This is especially true for the airborne lidar, which can cover transects like those in this study in minutes.However, the lidar depth penetration is limited to about 30 m in coastal waters like the west Florida shelf.
The correlation between lidar and glider results was not perfect, and one reason is probably the mismatch in sampling times and volumes.The overall correlation was greater on the northern transect than on the southern one, which is likely a result of a better match on the northern transect.The temporal variability in the distribution of scattering in the water is evident in the correlations between lidar data from different flights.These correlations are lower when there was a five-day temporal separation than when the separation was two or three days.

Discussion
This work highlights similarities and differences in the measurement of b bp by airborne lidar and glider-based in situ instruments.Both use a measurement of scattering at a single angle to infer b bp .This probably works better for the in situ scattering angle near 120 • , because there is less variability in the relationship between b bp and scattering at this angle than there is at the lidar scattering angle of 180 • [12][13][14][15][16][17].Both provide much greater sampling density than traditional ship surveys.This is especially true for the airborne lidar, which can cover transects like those in this study in minutes.However, the lidar depth penetration is limited to about 30 m in coastal waters like the west Florida shelf.
The correlation between lidar and glider results was not perfect, and one reason is probably the mismatch in sampling times and volumes.The overall correlation was greater on the northern transect than on the southern one, which is likely a result of a better match on the northern transect.The temporal variability in the distribution of scattering in the water is evident in the correlations between lidar data from different flights.These correlations are lower when there was a five-day temporal separation than when the separation was two or three days.
Another reason for moderate correlations might be that the assumption of a constant value for χ is not valid.It seems likely that the strong scattering near the bottom is primarily a result of suspended sediments with different particle sizes and refractive indices than the algal cells higher in the water column.This would affect the lidar estimates more than the glider estimates.The reason is that the measured variability of χ at 124 • , the angle of the glider instrument, is less than that at larger scattering angles.These measurements generally extend only to 170 • , but there is no reason to expect that the higher variability does not also extend to the lidar scattering angle of 180 • .
There are some remaining issues to be resolved for accurate measurements of b bp by airborne lidar.The biggest issue is probably the development of a robust lidar inversion technique [39].The technique used in this work assumed that the attenuation was slowly varying with depth.At the greatest depths, the attenuation of the lidar signal was 60 dB.Small errors in estimating this attenuation correspond to large errors in the corrected signal, because the correction is exponential.One possible solution is provided by the High Spectral Resolution Lidar (HSRL) that measures scattering and attenuation independently [33,40].
Another issue is the uncertainty in χ(180 • ).Typical measurements extent only out to 170 • .Some of these report χ(170 • ) > 1 [15,17], while others report χ(170 • ) < 1 [12,13,16].More recent laboratory instruments are even lower than most previous measurements, with χ(175 • ) < 0.01 for several algal species [21].There are also uncertainties in the wavelength dependence of this quantity [41][42][43].For a power-law spectrum with an exponent of unity, the lidar backscattering would be 22% greater than that from glider.A previous lidar study at 532 nm used χ(180 • ) = 0.5 [33].Here we used 1.0.This produced b bp from the lidar that was higher than that measured by the glider by 71% for low values and lower by 30% for high values.This would suggest that we have two different populations of scattering particles with χ(180 • ) = 0.58 and χ(180 • ) = 1.43, respectively.All of these values are within the uncertainty in χ(180 • ), and measurements specifically at this angle are required.This will require development of a new instrument, since current approaches to phase function measurements cannot be extended to 180 • .
For the cross-polarized channel, the situation is even more difficult.We not only need to know χ(180 • ), but also d(180 • ).There is probably even more uncertainty in this parameter, although some measurements of the polarization properties have been made [44][45][46][47].For spherical particles, d = 0 [48], so this parameter provides some information on the properties of the scatterers, but more work will be required to exploit this fully.Despite this difficulty, the cross-polarized channel provides greater contrast of high scattering regions and generally greater correlations with the glider data.A related issue is uncertainty in lidar calibration.If the particulate contribution to the signal is much larger than the pure water component, the effect is nearly linear.If the particulate contribution is small, however, small errors in estimates of total volume scattering coefficient can produce large errors in estimates of the particulate component.Our current calibration error is estimated to be more than 10%, and improving this calibration is an ongoing effort.The source of the error seems to be a large temperature difference between calibration laboratory and the actual operating conditions on the aircraft.
One promising future application of the lidar technique is the global measurement of b bp from space.Preliminary investigations have demonstrated that data from an existing aerosol lidar on the Cloud-Aerosol Lidar and Infrared Pathfinder Satellite Observations (CALIPSO) satellite can detect sub-surface oceanic scattering [49][50][51].This system, designed for atmospheric measurements, does not have sufficient range resolution for detailed profiles of oceanic backscatter, but airborne measurements have demonstrated that a future orbiting lidar designed for both atmospheric and oceanic profiling could provide valuable information.

Conclusions
The primary conclusion of this work is that airborne lidar and gliders provide consistent and complementary information on optical particulate backscattering distributions.The observed correlations, although moderate (R = 0.28 for the unpolarized lidar channel and R = 0.45 for the cross-polarized channel), show similar patterns in estimates of b bp despite differences in wavelength, scattering angle, and measurement volume.These measurements are complementary in the spatial-temporal scales that can be measured.The aircraft provides a measurement that is more nearly synoptic, but cannot measure to the depths or with the spatial detail provided by gliders.
At the same time, quantitative estimates of b bp from the lidar are limited by uncertainties in calibration, lidar retrieval, and χ(180 • ).The resulting error in the absolute estimates is probably not better than 3 dB.Improving understanding of the properties of χ(180 • ) will likely require development of a new in situ instrument.

12 Figure 1 .
Figure 1.Chart of the study area off the west coast of Florida, USA.Gray lines are depth contours in m.Red line is the glider track.Black lines are the flight track segments used in the analysis.Background is satellite estimate of bbp with values given by the color bar on the right.

Figure 1 .
Figure 1.Chart of the study area off the west coast of Florida, USA.Gray lines are depth contours in m.Red line is the glider track.Black lines are the flight track segments used in the analysis.Background is satellite estimate of b bp with values given by the color bar on the right.The Slocum G1 glider (Teledyne Webb Research) was deployed at 82.89 • W longitude, 27.28 • N latitude on 6 July 2015, reached the apex (83.43 • W, 26.71 • N) on 12 July and turned back towards the coast, and was finally retrieved on 17 July at 82.71 • W, 26.98 • N.During this period, it made a total of 3372 profiles (up or down) between 2 m below the surface and 2 m above the bottom (Slocum gliders constrain vertical travel using both pressure sensors and altimeters).An ECO-BBFL2 (WET Labs) "puck" in the central payload bay measured scattering of red (650 nm) light at a scattering angle of 124 • .The optical backscatter data were processed as follows: first, the sensor calibration was applied to the data to obtain the volume scattering function, β(124 • , 650 nm).The contribution from sea water was estimated as Remote Sens. 2017, 9, 379 5 of 12

Figure 2 .
Figure 2. Glider-measured particulate backscattering coefficient bbp as a function of depth and longitude according to the color table at the right.Plot shows the glider data binned into 0.01° latitude by 1 m depth bins for the east to west (top) and return transect (bottom).

Figure 2 .
Figure 2. Glider-measured particulate backscattering coefficient b bp as a function of depth and longitude according to the color table at the right.Plot shows the glider data binned into 0.01 • latitude by 1 m depth bins for the east to west (top) and return transect (bottom).

Figure 3 .
Figure 3. Particulate backscatter coefficient bbp as a function of depth and longitude according to the color table at the right.Top panel is the northern transect and bottom panel is the southern transect.Plot shows the lidar data averaged over the first three flights and binned into 0.01° latitude by 1 m depth bins.

Figure 3 .
Figure 3. Particulate backscatter coefficient b bp as a function of depth and longitude according to the color table at the right.Top panel is the northern transect and bottom panel is the southern transect.Plot shows the lidar data averaged over the first three flights and binned into 0.01 • latitude by 1 m depth bins.

Figure 4 .
Figure 4. (a) Scatter plot of binned particulate backscatter coefficient bbp as measured by lidar at 532 nm (unpolarized) as a function of those inferred from the glider data at 650 nm for the north transect.Grey squares denote a single data point within the region covered by the square (5 × 10 −5 m −1 in each dimension).Red squares denote multiple data points from two (lightest) to 20 (darkest shade) of 1228 total.Lidar data are from the 10 July flight.Solid line is the ideal 1:1 relationship for χ(180°) = 1.Short dashes show the 1:1 line, relative to the data, for χ(180°) = 0.5, and long dashes for χ(180°) = 2.0; (b) Similar plot of lidar attenuation, α, with darkest red representing 11 data points.

Figure 5 .
Figure 5. Correlations of binned data between flights for the two, three, and five day time differences among the first three flights for the north (black bars on the left) and south (gray bars on the right) transects.

Figure 4 .
Figure 4. (a) Scatter plot of binned particulate backscatter coefficient b bp as measured by lidar at 532 nm (unpolarized) as a function of those inferred from the glider data at 650 nm for the north transect.Grey squares denote a single data point within the region covered by the square (5 × 10 −5 m −1 in each dimension).Red squares denote multiple data points from two (lightest) to 20 (darkest shade) of 1228 total.Lidar data are from the 10 July flight.Solid line is the ideal 1:1 relationship for χ(180 • ) = 1.Short dashes show the 1:1 line, relative to the data, for χ(180 • ) = 0.5, and long dashes for χ(180 • ) = 2.0; (b) Similar plot of lidar attenuation, α, with darkest red representing 11 data points.

Figure 4 .
Figure 4. (a) Scatter plot of binned particulate backscatter coefficient bbp as measured by lidar at 532 nm (unpolarized) as a function of those inferred from the glider data at 650 nm for the north transect.Grey squares denote a single data point within the region covered by the square (5 × 10 −5 m −1 in each dimension).Red squares denote multiple data points from two (lightest) to 20 (darkest shade) of 1228 total.Lidar data are from the 10 July flight.Solid line is the ideal 1:1 relationship for χ(180°) = 1.Short dashes show the 1:1 line, relative to the data, for χ(180°) = 0.5, and long dashes for χ(180°) = 2.0; (b) Similar plot of lidar attenuation, α, with darkest red representing 11 data points.

Figure 5 .
Figure 5. Correlations of binned data between flights for the two, three, and five day time differences among the first three flights for the north (black bars on the left) and south (gray bars on the right) transects.

Figure 5 .
Figure 5. Correlations of binned data between flights for the two, three, and five day time differences among the first three flights for the north (black bars on the left) and south (gray bars on the right) transects.

Figure 6 .
Figure 6.Product of depolarization, d, and particulate backscatter coefficient bbp as a function of depth and longitude for the cross-polarized lidar return for the northern (top) and southern (bottom) transects.Because the lidar flights were more concentrated towards the earlier portion of the glider mission, the displayed lidar data were averaged over the first three flights for the northern transect and the second and third flights for the southern transect.They are binned into 0.01° latitude by 1 m depth bins.

Figure 6 .
Figure 6.Product of depolarization, d, and particulate backscatter coefficient b bp as a function of depth and longitude for the cross-polarized lidar return for the northern (top) and southern (bottom) transects.Because the lidar flights were more concentrated towards the earlier portion of the glider mission, the displayed lidar data were averaged over the first three flights for the northern transect and the second and third flights for the southern transect.They are binned into 0.01 • latitude by 1 m depth bins.

Table 1 .
Table of Pearson correlation coefficients, R, by flight date (2015) and transect using binned data where both lidar and glider values were non-zero.Dash denotes cases where the correlation was not statistically significant.For all other cases, p < 10 −5 ).

Table 1 .
Table of Pearson correlation coefficients, R, by flight date (2015) and transect using binned data where both lidar and glider values were non-zero.Dash denotes cases where the correlation was not statistically significant.For all other cases, p < 10 −5 ).