Terrestrial Laser Scanning for Vegetation Analyses with a Special Focus on Savannas

: Savannas are heterogeneous ecosystems, composed of varied spatial combinations and proportions of woody and herbaceous vegetation. Most ﬁeld-based inventory and remote sensing methods fail to account for the lower stratum vegetation (i.e., shrubs and grasses), and are thus underrepresenting the carbon storage potential of savanna ecosystems. For detailed analyses at the local scale, Terrestrial Laser Scanning (TLS) has proven to be a promising remote sensing technology over the past decade. Accordingly, several review articles already exist on the use of TLS for characterizing 3D vegetation structure. However, a gap exists on the spatial concentrations of TLS studies according to biome for accurate vegetation structure estimation. A comprehensive review was conducted through a meta-analysis of 113 relevant research articles using 18 attributes. The review covered a range of aspects, including the global distribution of TLS studies, parameters retrieved from TLS point clouds and retrieval methods. The review also examined the relationship between the TLS retrieval method and the overall accuracy in parameter extraction. To date, TLS has mainly been used to characterize vegetation in temperate, boreal/taiga and tropical forests, with only little emphasis on savannas. TLS studies in the savanna focused on the extraction of very few vegetation parameters (e.g., DBH and height) and did not consider the shrub contribution to the overall Above Ground Biomass (AGB). Future work should therefore focus on developing new and adjusting existing algorithms for vegetation parameter extraction in the savanna biome, improving predictive AGB models through 3D reconstructions of savanna trees and shrubs as well as quantifying AGB change through the application of multi-temporal TLS. The integration of data from various sources and platforms e.g., TLS with airborne LiDAR is recommended for improved vegetation parameter extraction (including AGB) at larger spatial scales. The review highlights the huge potential of TLS for accurate savanna vegetation extraction by discussing TLS opportunities, challenges and potential future research in the savanna biome.


The Role and Importance of Savannas
Savannas are heterogeneous ecosystems characterized by mixed physiognomies of woody and herbaceous layer [1]. Savanna ecosystems span one-fifth of the global land [2,3] which makes up approximately 20% of the terrestrial vegetation and contribute 30% of all terrestrial ecosystem gross primary productivity [4,5]. Savanna ecosystems also contribute significantly to the global carbon cycle with a net primary productivity UAV as well as terrestrial LiDAR sensors [27]. Spaceborne LiDAR provides the opportunity to make large-scale assessments of vegetation structure for global to regional biomass estimation [28]. Airborne LiDAR is also able to cover relatively large areas and measures vegetation structure with high accuracy [29]. Both spaceborne and airborne LiDAR systems are limited in detecting smaller trees and shrubs. The latter are key attributes in savanna ecosystems [30]. By using airborne or spaceborne LiDAR these attributes and are restricted to landscape level estimates. Mobile and UAV LiDAR sensors offer a faster approach for acquiring 3D point clouds. However, mobile LiDARs are constrained in extracting canopy and height attributes [31], whereas UAV LiDARs have difficulties in capturing tree trunks [32] and are also limited in flight time due to batteries [33]. An overview of the main advantages and disadvantages of the described monitoring technologies i.e., (field surveys, multi-spectral, hyperspectral, RADAR and LiDAR) platforms are also discussed in Béland et al. [27], Eisfelder et al. [34] and Galidaki et al. [35].

Terrestrial Laser Scanning (TLS)
Terrestrial Laser Scanning (TLS) has grown in interest because of its ability to produce 3D point cloud data for characterizing vegetation structure [36]. Accordingly, over the past decade, a considerable amount of research has been directed towards the 3D quantification of vegetation structure using TLS [37,38]. Vegetation structural parameters are difficult and laborious to measure in the field, especially in complex ecosystems like the savanna. However, TLS has proven efficient in deriving these parameters because it produces consistent and objective measurements with high precision. Furthermore, TLS measurements are relatively easy to interpret and parameter extraction can be automated [39,40].
A terrestrial laser scanner or ground-based LiDAR is a LiDAR system from which detailed 3D information called point clouds can be collected [41]. Using a laser beam, terrestrial laser scanners provide a high-resolution 3D view of the objects [36]. Depending on their range measurement principle, terrestrial laser scanners are classified into two types: phase shift or time of flight scanners [36,42]. Phase shift scanners measure distances by analysing the shift between the emitted and the received laser wave. In contrast, time of flight scanners compute distances based on the difference between the emission and reception of the laser pulse [42].
TLS measurements can be collected based on single or multiple scans. Using single scans, the scanner is placed at a single location within the study area whilst multiple scans are obtained when the scanner is placed at several positions within the test site [43]. For rapid and efficient extraction of vegetation parameters with reduced fieldwork and faster post-processing, single scans are preferred [44]. However, this approach is prone to occlusions (e.g., a tree being in the scan shadow of another tree or any other object) reducing the accuracy of vegetation structure retrieval and the ability to get a full 3D representation of an object [44]. For the best plot coverage and a complete 3D description of the objects under consideration, a multi-scan TLS setup is necessary [31,36].
Multi-scan TLS data allow for reliably retrieving common tree attributes such as Diameter at Breast Height (DBH), tree height, canopy structure and vertical height profiles [45,46]. In addition to these parameters, more detailed and complex vegetation attributes such as leaf water content [47], basal area and stem density [48], Above Ground Biomass (AGB) change [49], tree crown change [50] stem curve profiles [51], stem shrinkage [52], have been derived from TLS point clouds.
TLS provides unmatched 3D information on vegetation structure right down to branch and leaf scales, making it far superior to data obtained through traditional field work [38,53]. Using the intensity of the returned laser pulses, TLS can be used to map pigment concentration, photosynthetic capacity and water content of the vegetation [27]. It is also possible to separate wood from leaf material [54,55]. TLS offers a non-destructive approach to quantify canopy and stem volume with high accuracies, thereby enabling the estimation of AGB with reduced uncertainties as well as the development and improvement of reliable allometric equations [56,57]. TLS also enables the estimation of individual tree components [58] and it can also be employed as a calibration and validation dataset because of its ability to acquire data from the ground that is similar to traditional measures of vegetation structure [59,60].
The inherent heterogeneity of savannas presents a challenge regarding accurate vegetation characterization. To overcome this challenge, 3D data on the horizontal and vertical structure of the savanna are needed. TLS is able to account for savanna heterogeneity ( Figure 1) by providing detailed information on all savanna vegetation types and layers [61,62]. It therefore holds much potential for making holistic AGB assessments, including long and short stature vegetation [63][64][65].
Remote Sens. 2020, 17, x FOR PEER REVIEW 4 of 31 estimation of AGB with reduced uncertainties as well as the development and improvement of reliable allometric equations [56,57]. TLS also enables the estimation of individual tree components [58] and it can also be employed as a calibration and validation dataset because of its ability to acquire data from the ground that is similar to traditional measures of vegetation structure [59,60]. The inherent heterogeneity of savannas presents a challenge regarding accurate vegetation characterization. To overcome this challenge, 3D data on the horizontal and vertical structure of the savanna are needed. TLS is able to account for savanna heterogeneity ( Figure 1) by providing detailed information on all savanna vegetation types and layers [61,62]. It therefore holds much potential for making holistic AGB assessments, including long and short stature vegetation [63][64][65]. Different drivers can result in the loss of large trees in the savanna landscape [66] and changes in vegetation structure. The non-destructive nature of TLS makes it more so suitable for quantification of change in vegetation structure and reduction of uncertainties when estimating AGB [50,67]. The use of repeated TLS scans allows for monitoring these and other losses (and potentially gains) in savanna AGB over time. Multi-temporal TLS therefore holds the potential to support carbon accounting programmes such as REDD+.
While TLS is able to capture the 3D configuration of vegetation at high spatial resolution, data can only be acquired for a limited areal extent and data acquisition usually requires extensive field work [68]. This problem is solved by the integration of TLS with other remote sensing data to ensure the coverage at landscape scales. For example, landscape studies were conducted in the savanna by the integration of TLS with airborne Li-DAR [69], TLS with MODIS satellite data [70] and TLS with ALOS PALSAR L band data [62]. Moreover, TLS scans frequently feature occlusions ("shadows") caused by objects close to the scanner which, in turn, block the view of other objects further away. Although occlusions are not a major problem in open savannas, they can be minimized by the use of multiple TLS scans [30,64]. Another shortcoming of TLS systems is that the point density decreases with distance from the scanner, an effect called beam divergence [68,71] which causes the point cloud to be unreliable at a certain distance.

Overall Goal and Objectives of the Review
This paper aims to review the scientific literature on the use of TLS for vegetation analyses. It puts a special but not exclusive geographic focus on savannas in order to establish the current status quo in savanna vegetation parameter extraction. In contrast to Different drivers can result in the loss of large trees in the savanna landscape [66] and changes in vegetation structure. The non-destructive nature of TLS makes it more so suitable for quantification of change in vegetation structure and reduction of uncertainties when estimating AGB [50,67]. The use of repeated TLS scans allows for monitoring these and other losses (and potentially gains) in savanna AGB over time. Multi-temporal TLS therefore holds the potential to support carbon accounting programmes such as REDD+.
While TLS is able to capture the 3D configuration of vegetation at high spatial resolution, data can only be acquired for a limited areal extent and data acquisition usually requires extensive field work [68]. This problem is solved by the integration of TLS with other remote sensing data to ensure the coverage at landscape scales. For example, landscape studies were conducted in the savanna by the integration of TLS with airborne LiDAR [69], TLS with MODIS satellite data [70] and TLS with ALOS PALSAR L band data [62]. Moreover, TLS scans frequently feature occlusions ("shadows") caused by objects close to the scanner which, in turn, block the view of other objects further away. Although occlusions are not a major problem in open savannas, they can be minimized by the use of multiple TLS scans [30,64]. Another shortcoming of TLS systems is that the point density decreases with distance from the scanner, an effect called beam divergence [68,71] which causes the point cloud to be unreliable at a certain distance.

Overall Goal and Objectives of the Review
This paper aims to review the scientific literature on the use of TLS for vegetation analyses. It puts a special but not exclusive geographic focus on savannas in order to establish the current status quo in savanna vegetation parameter extraction. In contrast to existing reviews in the TLS literature [72,73], this study presents the current TLS methods in practice and identifies those suitable for accurate biomass assessments in the savanna biome. To address this gap, our meta-analysis takes a detailed look at the geographic distribution of TLS studies, the parameters retrieved from TLS point clouds, known retrieval methods as well as the main opportunities and challenges arising from the studied literature. The remainder of the paper is structured as follows: Section 2 presents the approach and Section 3 the main findings of the review. The main discussion points and conclusions derived from the review are presented in Sections 4 and 5, respectively.

Materials and Methods
A systematic literature review was conducted in Web of Science [74]. Access to its catalogue was granted through the Thüringer Universitäts-und Landesbibliothek Jena (ThULB). The advanced search functionality in Web of Science was used to develop and apply a set of predefined queries. The search included all journals in the Web of Science Core Collection™ database. Peer-reviewed conference papers were also included. Searching for keywords in the title (TI) and in some instances in all fields (ALL) (when a query returned fewer than ten articles) was performed with a time frame up to May 2020. The time span for the search was all years that is from 1945 to 2020. In the event that a search query returned more than 100 articles, the search was refined by the research areas forestry or remote sensing to narrow down on specific studies employing TLS for vegetation parameter extraction. Each query consisted of different keywords and related synonyms (Table 1) which were combined through logical operators (i.e., "AND", "OR"). An example of one of the search queries developed is: TI = ((TLS OR Terrestrial LiDAR OR Terrestrial Laser Scann *) AND (Tree Structur *)). This search query would return all journal articles that have either the word or terrestrial LiDAR or terrestrial laser scanner or terrestrial laser scanning and the word tree structure or tree structural in their title.
Following the above search scheme, a total of 158 research articles were initially retrieved from Web of Science. In a second step, certain papers were retained for the review according to a prescribed set of criteria. The criteria used to refine the initial search results were as follows: The study under consideration focused on the extraction of one or more vegetation parameters from TLS point clouds. For example, a study was only considered if parameters like the DBH or AGB were derived on the basis of TLS point clouds [75]; The study under consideration used TLS point clouds or products as a calibration and/or validation data set for other remote sensing technologies (e.g., UAV LiDAR [32] airborne LiDAR [76] and ALOS PALSAR [62]; The study under consideration reviewed TLS as a remote sensing technology [39]; The study under consideration conducted technical experiments with TLS data to investigate, e.g., how specular reflectors affect distance in TLS measurements [77] or which scan setup (single vs. multiple scans) in the field is best suited for a particular application [51].
This resulted in a final number of 113 reviewed publications. To qualitatively and quantitatively assess the final selection of papers, a comprehensive meta-analysis was conducted based on 18 predefined study attributes. Besides author(s), year of publication and publishing journal, the list of reviewed meta-parameters included: study area(s) (country, continent, biome); type of laser scanner used (TLS model/device); range finder of the scanner (phase shift or time of flight); type of TLS data used (xyz and intensity); scanner setup (TLS settings for scanning); field setup (use of reflectors, GPS/dGNSS); number of scans (single vs. multiple scans); parameters retrieved (e.g., DBH, vegetation height, crown diameter); retrieval method (algorithm used); number of studied trees and shrubs (total number of trees/shrubs measured in the research); performance metrics such as coefficient of determination (R 2 ), Root Mean Square Error (RMSE) and bias; main discussion points (e.g., advantages and limitations of a used approach); and lastly research gaps and potential avenues for future research. The above search efforts were undertaken to ensure that all relevant TLS literature was collected. The full list of papers considered and finally selected in this review is available in Table S1 (supplementary material).

General Overview
Of the 113 publications used for the detailed review, 80% of the publications focused on the extraction of vegetation parameters from TLS point clouds, 12% used TLS as auxiliary data (e.g., TLS data used to validate airborne LiDAR) [78][79][80] and 8% applied TLS for technical experiments (e.g., reflectance modelling using TLS intensity data) [81][82][83]. Among the 113 papers, 97 were publications from peer-reviewed journals whilst 16 were peer-reviewed proceedings. The most popular journals were Remote Sensing (20 studies), Remote Sensing of Environment (9 studies), Agricultural and Forest Meteorology (8 studies) and ISPRS Journal of Photogrammetry and Remote Sensing (6 studies). Most proceedings papers stem from the International Geoscience and Remote Sensing Symposium (IGARSS), with 9 studies. The geographic distribution of the reviewed studies by country is shown in Figure 3. To date, most studies were conducted in the United States, China and Finland. From a continental perspective, Europe has the highest number of studies (38%) [84][85][86] followed by North America (24%) [46,59,87] and Asia (19%) [88][89][90]. Africa (6%), Australia (5%) and South America (7%), with more than half of their landmass belonging to the savanna biome [1,2], had few TLS studies [64,91,92].

Temporal and Spatial Patterns
The geographic distribution of studies by biome (after Olson et al. [93]) and the percentage of reviewed studies per biome are shown in Figures 3 and 4. Studies that were conducted in more than one biome were counted multiple times and four laboratory studies were excluded from this analysis, resulting in a sample size of n = 114. The reviewed papers covered a total of 11 biomes. While most studies were conducted in temperate forests (47%) [75,94,95], much less papers were published in boreal (16%) [96][97][98], tropical and subtropical forests (10%) [99][100][101] as well as grasslands and savannas (9%) [70,91,92]. The cumulative total for the savanna biome was 13%, including 4% of savanna research conducted in temperate regions ("Temperate Grasslands Savannas and Shrubland"). The geographic distribution of the reviewed studies by country is shown in Figure 3. To date, most studies were conducted in the United States, China and Finland. From a continental perspective, Europe has the highest number of studies (38 %) [84][85][86] followed by North America (24 %) [46,59,87] and Asia (19 %) [88][89][90]. Africa (6 %), Australia (5 %) and South America (7 %), with more than half of their landmass belonging to the savanna biome [1,2], had few TLS studies [64,91,92].
The geographic distribution of studies by biome (after Olson et al. [93]) and the percentage of reviewed studies per biome are shown in Figure 3 and Figure 4. Studies that were conducted in more than one biome were counted multiple times and four laboratory studies were excluded from this analysis, resulting in a sample size of n = 114. The reviewed papers covered a total of 11 biomes. While most studies were conducted in temperate forests (47 %) [75,94,95], much less papers were published in boreal (16 %) [96][97][98], tropical and subtropical forests (10 %) [99][100][101] as well as grasslands and savannas (9%) [70,91,92]. The cumulative total for the savanna biome was 13 %, including 4 % of savanna research conducted in temperate regions ("Temperate Grasslands Savannas and Shrubland").

TLS Point Cloud Pre-Processing
The scanner in use usually comes with software for pre-processing the point cloud; for example, Riegl scanners come with Riscan Pro, Faro with FARO Scene and Leica with Leica Cyclone. The first stage in prepossessing if a multi scan setup was used is to conduct coarse registration and georeferencing to the local coordinate system using the TLS instruments provide two types of data that can be used for forestry and vegetation studies. These are 3D coordinates (XYZ data, point clouds) and the intensity of the reflected signal. The signal can either be discrete or full-waveform. Discrete data provide single or multiple returns for each laser pulse [76,130]. Full -waveform contain measurement of the reflected laser beam as a function of range [76,130]. These two data types can be integrated with auxiliary data from the field, including RGB images from digital cameras. Most studies used XYZ data (85 studies) [131][132][133] whereas intensity information was considered much less (8 studies) [129,134,135]. A triple combination of both data types and RGB images (2 studies) [121,136] as well as a combination of XYZ and RGB (3 studies) [75,111,137] was not commonly observed. Instead, a number of studies took advantage of XYZ and intensity data (15 studies) [127,138,139]. No paper presented results based on a combination of TLS intensity data and RGB images.

TLS Point Cloud Pre-Processing
The scanner in use usually comes with software for pre-processing the point cloud; for example, Riegl scanners come with Riscan Pro, Faro with FARO Scene and Leica with Leica Cyclone. The first stage in prepossessing if a multi scan setup was used is to conduct coarse registration and georeferencing to the local coordinate system using the retroreflectors as tie points [43,96]. Fine registration can also be achieved using multi-station adjustment through an iterative closest point algorithm in Riscan Pro software [43,140]. Multi-station adjustment requires a minimum of 3 TLS setups with a minimum overlap of 30%. The algorithm uses surface and point normals to compute the adjustment [141]. The registered point cloud is then clipped to the radius of the plot in the case of circular plots or to the extent of the plots or to remove low density points at the plot edges [140,142].
The final pre-processing stage involves filtering the point cloud to remove noise from the data [43,121,142]. Noise and outlier removal is done to increase accuracy and reduce errors when extracting information from the point cloud data [115]. The noise can be classified into internal and external noise, internal noise being the inherent noise from the scanning device and external noise from the reflective nature of the scene [143,144]. The common noise points from vegetation are leaves, extremely high or low points, and from occlusions [115,144]. The filtering is performed using various approaches such as statistical, neighbourhood, projection, signal and partial differential based filtering techniques [144]. For example, in the case of statistical outlier removal, the filtering reduces the error on the point cloud by computing the average distance to its neighbours and rejects points that are further away from the average distance [115,144]. Point cloud filtering can be performed in software packages such as Cloud Compare, LAStools, MeshLab and programming libraries such as Point Cloud and Open Graphics Library [143]. After pre-processing various algorithms are then applied to extract the vegetation parameters.

Methods Used with TLS Point Clouds
Of the 113 papers reviewed, 87 papers focused on the extraction of vegetation parameters from TLS point clouds. Of the 87, only 15 studies worked in the savanna biome [39,145,146]. The most frequently employed methods to derive vegetation attributes are the RANdom SAmple Consensus (RANSAC) algorithm for extracting DBH, stem curve profiles and the detection of stems [97,147], the use of the highest z coordinate of the point cloud for estimating heights [61,148], voxel-based and radiative transfer models for assessing LAI [149,150], Canopy Height Models (CHMs) for delineating crown attributes [91,92], and Quantitative Structure Models (QSMs) for computing tree volume and branch parameters [151,152]. Popular software packages to implement and work with these methods are Lastools [153], Matlab [154], R [155] and Python Programming [156], Cyclone [157], FARO Scene [158], RiscanPro [159], CompuTree [160] and Cloud Compare [161]. For evaluating the above extraction techniques, performance metrics such as R 2 , RMSE and bias are typically used [51].

Vegetation Parameters Extracted from TLS Data
The vegetation parameters that can be retrieved from TLS data can be broadly classified into primary and secondary parameters [22]. Primary parameters are those directly derived from the TLS point cloud, such as DBH, height, volume and tree canopy characteristics [22]. Secondary parameters can only be estimated indirectly and are derivatives of primary parameters. They are usually obtained through modelling [22]. Examples of secondary parameters are AGB, basal area, stem density and LAI [22]. Besides the common vegetation parameters e.g., DBH and height, some studies focused on extracting unique and complex vegetation parameters such as tree planar projection [162], the elevation of angle and azimuth of stem inclination [52], clumping index [163], ground cover [92] and structural loss [164].As shown in Figure 6, most of the reviewed studies retrieved primary parameters, including DBH (41), height (32), tree canopy features (e.g., crown diameter, canopy cover) (20), trees or tree stems (20) and tree volume (15). With only five and six articles, respectively, branch parameters and stem curve profiles gained the least attention. Of the secondary parameters, LAI was the most extracted parameter (21), followed by AGB (11), basal area (5) and stem density (3). Details regarding specific parameters and the methods to retrieve them are provided in the following subsections. Particular emphasis is put on savanna ecosystems.

DBH
From the reviewed papers, only one study derived DBH in the savanna [92]. Muir et al. [92] employed circle fitting to single-scan TLS data at heights between 1.28 m and 1.32 m above the Digital Elevation Model (DEM) in an open woodland site in Central Western Queensland Australia. They compared the resulting stem diameter measurements with data collected in the field and obtained an R 2 value of 0.77 [92]. Slightly better performances are often observed for DBH extraction in temperate, tropical and boreal forests, with R 2 values ranging from 0.81 to 0.99 [94,165,166] when compared to field reference data. For a pine woodland test site in France, least-squares circle fitting was reported to be the most accurate method (RMSE = 0.019 m) for estimating DBH when compared to least squares cylinder fitting and circular Hough transformation [85]. Similarly, Calders et al. [40] and Chen et al. [167] obtained R 2 values of 0.97 when using least squares circle fitting for DBH estimation with a multi-scan TLS scan with 5 positions in a eucalypt open forest and boreal forest respectively. Yurtseven et al. [148] obtained an R 2 value of 0.99 with a multi-scan with 8 positions in a Mediterranean forest employing randomised hough transformation with least square regression. In general, multiple TLS scans and a lower stem density yielded the best results [114,168,169].

Vegetation Height
Tree height estimation in the savanna was reported in five studies [30,62,91,92,145]. Odipo et al. [62] and Muir et al. [92] employed the use of CHMs to estimate tree height from multiple and single TLS scans (30 positions and single scan) and obtained R 2 values of 0.64 and 0.98 respectively when compared to ground referenced data. Singh et al. [30] observed an RMSE of 0.25 to 1.45 m based on multi-scan Long Range -TLS (LR-TLS) with 4 positions. They computed heights by measuring the vertical distance between treetops and the stem base. Kirton et al. [145] employed cylinder fitting to extract tree height and obtained a standard error of ±0.002 m using a TLS single scan. It was observed in this study that it is more challenging to derive the height of savanna shrubs than it is for savanna trees. Muir et al. [92] also reported that shrub heights are difficult to calculate from TLS data, with R 2 values of 0.381 and 0.08 for maximum and average shrub height, respectively. In contrast to this observation, the extraction of height from short stature vegetation was achieved with reasonable accuracy in montane areas as well as desert and xeric shrubland biomes [170]. Vrlingie et al. [170] applied a 2-D spatial wavelet analysis on a TLS derived CHM to extract shrub height and achieved an R 2 of 0.94 when compared to field measured shrub height.

Stem Detection
The detection of stems from TLS point clouds is an important first step in processing, to enable extraction of other vegetation parameters [171]. Only one study [147] from the review was conducted in the savanna biome. Burt et al. [147] employed treeseg algorithm (which extracts tree point clouds from larger point clouds) on a tropical and on an open vegetation cloud using TLS multi scans with 5 positions. They achieved 70% and 96% stem detection accuracy, respectively [147]. The detection of stems was achieved with high accuracies in temperate and boreal forests [138,171]. Kelbe et al. [138] used voxel stem segment modelling on a single TLS scan dataset acquired using low resolution TLS in various forest types in New England. Stems were detected with accuracies of (R 2 = 0.99; RMSE = 0.16 m) [138]. In a boreal forest Zhang et al. [171] employed connected component segmentation using both single and multi-scan with 5 positions. They achieved stem detection accuracies of 93% for single scan and 99% for multi-scan TLS setup when compared to reference data [171]. To date, most of the commonly used algorithms (e.g., density-based clustering [172], Hough transformation [85] and RANSAC cylinder fitting [48,173] used for stem detection achieved stem detection accuracies of 70% to 99% when applied in temperate, tropical and boreal forests.

Crown Attributes
Among the tree crown attributes dealt with in the scientific literature are canopy cover, canopy height/length, crown width/diameter and tree crown change. In the savanna biome, extraction accuracies for trees were better as compared to shrubs. Singh et al. [30], Odipo et al. [62] and Muir et al. [92] employed CHMs to determine canopy cover. Singh et al. [30] achieved an RMSE of 5.7-15.9% at distances up to 500 m with 4 scan positions, whilst Odipo et al. [62] and Muir et al. [92] achieved R 2 of 0.56 and 0.93 as compared to the ground referenced data with 30 and single scan positions respectively. Lower accuracies were obtained when estimating shrub canopy cover, with Muir et al. [92] reporting an R 2 of 0.23 when compared to field measurements. Yet, the application of 2D spatial wavelet analysis achieved an R 2 of 0.51 for 36 shrubs in a desert and xeric shrubland environment [170]. In this study, shrub crown area was calculated using spatial wavelet analysis using the detected radius while the actual shrub crown area was determined by measuring the minor and major axis and then calculating the crown as an ellipse [170]. The use of different algorithms for accurate extraction of canopy cover was also investigated by Yurtseven et al. [148] in a mediterranean woodland and scrub. They employed convex and concave hulls and they concluded that the concave planar underestimates canopy cover area as compared to the convex hull. A method to quantify canopy cover change was considered by Olivier et al. [50] in a sugar maple and balsam fir forest in Canada. They used bi-temporal multi-scan TLS data to identify vegetation boundaries, extract new material formed or displaced between the two-time period [56].

Tree Volume and Branch Parameters
Volume of standing trees or above ground volume is total stem wood and branches [174]. Branch parameters are parameters like branch total length, branch diameter, branch insertion angle, branch depth into the crown and branch radius. Over the years, a range of algorithms has been developed to compute tree volume and branch parameters from TLS point clouds [151,175]. However, none of the corresponding studies was conducted in the savanna biome. QSMs are the most common algorithms used in literature [96,151,175]. They have the ability to provide reliable volumes estimates of stem and branches of trees, but also have limitations in reconstructing leaves [176]. Figure 7 shows an example of a 3D point cloud and the resulting QSM. Branching structure and branch lengths were determined in boreal and tropical forests using QSM [151,175]. Kaasalainen et al. [175] used a multi scan with 2 positions to reconstruct branching structure and concluded that branching structure can be reproduced with ±10% accuracy when compared to laboratory reference measurements. Lau et al. [151] used a multi-scan with 13 positions and successfully reconstructed 87% of the branch lengths. They compared the reconstructed branch lengths to manually measured branches from destructively harvested trees [151]. For the estimation of shrub total and green biomass, Olsoy et.al [54] employed voxel-based and 3D convex hull algorithms to first determine shrub volume in a xeric sagebrush ecosystem in Southern Idaho, USA using multi scan with 2 positions. They then modelled shrub total and green biomass by subsetting the point cloud into green and non-green woody components based on the difference in their reflectivity. Their findings showed that convex hull algorithms (R 2 = 0.92, R 2 = 0.83) performed slightly better than the voxel-based method (R 2 = 0.86, R 2 = 0.73) in estimating total and green biomass respectively [54]. The TLS derived shrub biomass was compared to the destructive samples of shrubs [54]. These and other volume-related algorithms (e.g., bounding cuboids, outer hull models, square-based columns) have also been compared for test sites in the temperate and tropical regions [101]. It was found that convex hull techniques tend to overestimate while the square-based column method underestimates volume. Moreover, voxel-based estimates are mainly affected by the point density and level of occlusions in the point cloud [101].

Stem Curve Profiles
Stem profiles or stem taper is the "stem diameter measured as a function of height" [179]; that is, how the diameter of the tree changes at different heights along the stem. Six studies used TLS point clouds to determine stem curve profiles, five of the studies were conducted in boreal forests [48,51,52,162,166] and one was conducted in temperate broadleaf and mixed forest [58]. The methods used to extract stem curve profiles from TLS point clouds are either circle or cylinder fitting at different height bins [48,51,52,58,162,166]. Maas et al. [58] extracted stem curve profiles using circle fitting at multiple heights and obtained an RMSE of 4.7 cm when compared to a harvested stem. Liang et al. [51] observed that most algorithms used for the extraction of stem curve profiles results in an RMSE of 1.3 and 6.0 cm for single scan data and 0.9 and 5.0 cm from multi scan data for three different stem densities (i.e. low, medium and high stem densities) in a boreal forest. The RMSE values obtained are a function of both the stem density and the number of scan positions employed [51].

AGB
AGB is defined as "the total amount of biological material (oven-dried) present above the surface in an area" [180]. AGB is the most important secondary attribute derived from TLS point clouds. The accurate extraction of all other parameters is a necessity in improving tree biomass estimation. A total of five studies estimated AGB in the savanna using TLS [62,64,70,90,126]. Zimbres et al. [64] predicted plot AGB of three vegetation types in the Brazilian cerrado. The TLS derived AGB was validated against the AGB

Stem Curve Profiles
Stem profiles or stem taper is the "stem diameter measured as a function of height" [179]; that is, how the diameter of the tree changes at different heights along the stem. Six studies used TLS point clouds to determine stem curve profiles, five of the studies were conducted in boreal forests [48,51,52,162,166] and one was conducted in temperate broadleaf and mixed forest [58]. The methods used to extract stem curve profiles from TLS point clouds are either circle or cylinder fitting at different height bins [48,51,52,58,162,166]. Maas et al. [58] extracted stem curve profiles using circle fitting at multiple heights and obtained an RMSE of 4.7 cm when compared to a harvested stem. Liang et al. [51] observed that most algorithms used for the extraction of stem curve profiles results in an RMSE of 1.3 and 6.0 cm for single scan data and 0.9 and 5.0 cm from multi scan data for three different stem densities (i.e. low, medium and high stem densities) in a boreal forest. The RMSE values obtained are a function of both the stem density and the number of scan positions employed [51].

AGB
AGB is defined as "the total amount of biological material (oven-dried) present above the surface in an area" [180]. AGB is the most important secondary attribute derived from TLS point clouds. The accurate extraction of all other parameters is a necessity in improving tree biomass estimation. A total of five studies estimated AGB in the savanna using TLS [62,64,70,90,126]. Zimbres et al. [64] predicted plot AGB of three vegetation types in the Brazilian cerrado. The TLS derived AGB was validated against the AGB values obtained from allometric equations. The R 2 values obtained were 0.92 and 0.88 for the woodland savanna dry and rainy season respectively and 0.58 for the forested savanna. Due to occlusions, no conclusive results were obtained for the gallery forest [70]. Cuni-Sanchez et al. [70] estimated AGB change in five different vegetation types using vertical plant profiles extracted from a multi-scan TLS over a period of 20 years in Gabon. However, the savanna plots were not assessed because all the trees had a diameter of less than 10 cm [70]. Odipo et al. [62] also estimated AGB change in a south african savanna using TLS derived canopy cover and height metrics for plot level AGB and then further extrapolating to a wider scale using multi-temporal L band SAR. The TLS derived metrics were validated with field measured parameters. Significant relationships were obtained between the TLS derived predictors (canopy cover & height) with field biomass (R 2 = 0.93). Seven variables extracted from TLS data were used to build regression models with field measured AGB to estimate grass biomass in China [90]. The regression methods used were Simple Regression (SR, Stepwise Multiple Regression (SMR), Random Forest (RF) and Artificial Neural Network (ANN). Results obtained showed that the highest prediction of biomass was obtained using SMR model (R 2 = 0.84), followed by SR (R 2 = 0.80), RF (R 2 = 0.78) and lastly ANN (R 2 = 0.73) [90]. Cooper et al. [126] investigated the application of Structure from Motion (SfM) photogrammetry and TLS for the estimation of grass biomass and compared this to destructive harvest grass biomass. The R 2 values for the TLS derived grass biomass was 0.46 and total biomass was 0.57, whilst for the SfM it was 0.54 for the grass biomass and 0.72 for the total biomass [126]. TLS data have also been used to derive tree and grass AGB change [49,115]. Srinivasan et al. [49] extracted various tree parameters (including AGB) from loblolly pines in Texas, USA by the use of multi-temporal TLS data. The best results (R 2 = 0.50, RMSE = 10.09 kg) were obtained by directly estimating AGB change from the point cloud when compared to field measured AGB change. Guimarães-Steinicke et al. [115] also investigated grass biomass temporal change in Thuringia, Germany. They used 25 TLS derived metrics to model biomass [115].
While most of the reviewed studies focused on the estimation of tree biomass, only few papers dealt with shrub biomass. Shrub biomass was investigated by Greaves et al. [59] in the arctic tundra. They employed a leave one out cross-validation technique of the canopy volume against the harvested shrub biomass to establish predictive relationships between the TLS canopy volume and harvested shrub biomass and between Airborne Laser Scanning (ALS) canopy volume and TLS derived shrub biomass estimates. The TLS produced more accurate predictions of shrub biomass as compared to the ALS (TLS R 2 = 0.78 and ALS R 2 = 0.62). Li et al. [181] merged TLS and ALS to estimate the biomass of sagebrush in southwest Idaho, USA by building predictive models. TLS derived biomass was used for calibration of a biomass predictive model with an R 2 of 0.87. The TLS derived shrub volume was compared to the destructive shrub biomass measurements (R 2 = 0.88) [181].

LAI
LAI is defined "as the ratio of leaf area to per unit ground surface area" [39,130]. A study was conducted in the savanna biome by Béland et al. [39]. They applied a voxelbased approach making use of the contact frequency method on six trees. To this end, they employed two multi-scan setups with 2 and 3 positions and compared the TLS derived estimates against direct measurements obtained from leaf harvesting. The leaf area ranged from 30 to 530 m 2 whilst crown LAI was 0.8 and 7.2 with a general bias of 14%. To ensure an accurate extraction of LAI, the green and non-green components were distinguished in the TLS data based on a separation threshold applied to the return intensities [39]. Olsoy et al. [182] estimated the LAI of sagebrush from TLS data with 2 scan positions and found that the estimated LAI was highly correlated with their reference field data (R 2 = 0.78).

Basal Area
Basal area is defined as a "cross-sectional area of all stems in a plot measured at breast height and usually expressed per unit of the land" [183]. Five studies estimated basal area from TLS point clouds, with four being conducted in a temperate broadleaf and mixed forest [75,78,85,184] and one from the boreal zone [48]. No study has been published yet for the savanna biome. Yrttimaa et al. [48] and Tansey et al. [85] derived basal area from TLS point clouds by first calculation basal area for a single stem, then scaling it to one hectare. The TLS derived basal area was compared to the field derived basal area which was derived by considering the cross-sectional area of a tree to be circular [48]. The R 2 observed were 0.87, 0.86 and 0.63 for a plot radius of 6, 11 and 16 m respectively [48]. Côté et al. [184] used an architectural model called LiDAR to Tree Architecture (L-Architect) by reconstructing forest stands with scans of individual trees. The L-Architect derived basal area was compared to the measured values of basal area to assess accuracy. A relative difference of −0.01% was obtained between the L-Architect and field measured basal area [184]. A methodology based on voxel data structure was applied to derive the basal area of individual trees in a heterogeneous vegetation structure in Washington Park Arboretum [75]. The TLS derived basal area was derived by fitting a 3D cylinder primitive to the trunk assuming that the trunk was a geometric circle. The TLS derived basal area was compared to the manually measured basal area. The TLS derived basal area was underestimated compared to the field-based methods for conifers, deciduous and mixed stands (Conifer: Field = 12.3116 m 2 , TLS = 6.9217 m 2 ; Deciduous: Field = 1.4275 m 2 , TLS = 1.0172 m 2 & Mixed: Field = 1.8431 m 2 , TLS = 1.575 m 2 ) [75]. Aijazi et al. [78] used a super voxel segmentation method to estimate basal area and concluded that parameter estimation error ranged from 1.6 to 9% from TLS point clouds.

Stem Density
Stem density is defined as "the number of trees per hectare" [185]. Three of the reviewed studies estimated stem density, with one being performed in a savanna ecosystem [92]. Stem density is derived from the number of stems identified in the scanned area and comparing it to the field derived stem density [48,85,92]. Yrttimaa et al. [48] varied the plot size in order to investigate its effects on stem density. The smaller the plot size, the higher the correlation of the TLS-derived stem density with field measurements (r = 6 m | R 2 = 0.77; r = 11 m | R 2 = 0.67; r = 16 m | R 2 = 0.38). Muir et al. [92] determined stem density from TLS point clouds as well. They achieved an R 2 of 0.1 and reported a large RMSE of 130.9 trees/hectare that was attributed to plot complexity.

Geographical Trends in the TLS Literature
From a global perspective, most TLS studies have been conducted in Europe, North America and China (Figure 3). Despite their large geographical extent in Africa, South America and Australia, savannas have rarely been investigated in these areas. In the early 2000s, TLS-based vegetation studies were mainly conducted in Europe and North America (Figure 2). From 2009 onwards, TLS studies expanded into the rest of the world, including Africa, Asia, Australia and South America. An increasing collaboration among TLS research groups from different parts of the world can explain this observation [57,100,147,171]. To further advance TLS research in savannas and to learn more about their ecology, building scientific capacity in financially constrained countries is key.
Savannas cover ca. 50% of Africa's landmass. Yet, only 6% of the reviewed studies were conducted in Africa. Of those studies, the majority focused on South Africa. Only a few articles presented results for other African countries, including Mali [39], Ethiopia [186] and Gabon [70]. One reason for this observation is that TLS research in Africa is mainly constrained by financial limitations. The acquisition and processing of TLS data usually require expensive equipment and software [22]. Moreover, it necessitates trained personnel, which is limited in most third-world countries [176]. With the advent of low-cost terrestrial laser scanners [65,187,188], more TLS research can be expected in the future for Africa.
Since savannas cover large geographical areas, TLS data can usually not be used by itself to study an entire region due to its limited footprint. Instead, an integration of TLS point clouds with other geospatial data is necessary for large-scale assessments. In the past, the creation of wall to wall maps of savannas has been achieved by combining TLS with L-band SAR [62] and airborne LiDAR [69]. Currently, the existence of freely available satellite data from Sentinel-1/2 and Landsat 5-8, as well as coarser resolution imagery from MODIS [70] can resolve upscaling issues from fine spatial resolution from TLS to medium resolution from Landsat and Sentinel then to coarse resolution using MODIS. The planned launch of BIOMASS by the European space agency and Multi-footprint Observation LiDAR and Imager (MOLI) by JAXA after Global Ecosystems Dynamics Investigation (GEDI) and Ice Cloud & Elevation Satellite-2 (ICESat-2) will present an opportunity for landscape-level studies of the savanna [60]. Based on these and similar data sets, TLS point clouds can be used in wide-area mapping for calibration and validation purposes [60].

Challenges & Opportunities
Single and multiple scans (five, four, three and two positions) were found to be the most commonly used TLS setups in the reviewed literature. Single scan approaches have been used successfully to estimate DBH [138,166], height [92] and to detect stems [189]. Single scan inventories received a lot of attention because they allow for fast and less labour intensive data acquisitions and do not require the registration of multiple point clouds [109,112]. However, single scan approaches are also more prone to occlusions [43]. Data from multi-scan TLS campaigns were shown to yield higher accuracies and reduced error margins [45,139]. Therefore, multiple TLS scans with larger plot sizes (>15 m in radius) provide the only opportunity to adequately capture the heterogeneous nature of savannas and to characterize the full spectrum of savanna vegetation structure [92]. Depending on the parameters to be extracted, the requirements to be met and the available resources, the choice between a single-or multi-scan TLS setup should be made carefully. For example, long range single scans give reliable data in the dry season (leaf-off) and not in the wet season (leaf-on) [30].
DBH is one of the most reliable proxies for biomass [190]. This is why it is also the most frequently measured quantity not only by foresters, but also with regard to the reviewed literature. It has been shown that DBH can be delineated automatically based on multiple TLS scans [40]. However, these techniques have not yet been adjusted or tested for more complex vegetation structures [88]. To date, most algorithms to retrieve DBH (and also stems and stem curve profiles) assume a circular or cylindrical stem geometry. However, these rather simple geometries are not well-suited to describe the short stature vegetation and deformed trees that are typically found in savannas [40,191]. While a number of DBH extraction techniques (e.g., RANSAC algorithm [186], point cloud slicing at 1.3 m [75], least-squares circle fitting [52] and Hough transformation [86] exist), they have only been developed and demonstrated in temperate and boreal forests. For savannas, only the circle fitting method has been explored yet [92].
Height was extracted from TLS point clouds in the savanna, mostly through the employment of CHMs to achieve the goal. Muir et al. [92] obtained an R 2 of 0.97 and 0.38 for TLS-based estimates of tree and shrub heights, respectively. Small stature woody vegetation is often not well captured by CHMs, which leads to an underestimation of its height when interpolation is used to create the canopy surface [170]. This issue has not yet been adequately addressed, and more accurate height assessment techniques still need to be developed [170]. Potential ways to achieve this would be to elevate the scanner above the vegetation layer or place the scanner on a suitable vantage position [30,92], to capture TLS data of shrubs with higher point density (e.g., 16 pts per m 2 , [170]) and to distinguish between trees and shrubs in the TLS point cloud and to create two corresponding tree-and shrub-related CHMs that can be analysed separately [92].
Other methods for vegetation height extraction are available, including the combined analysis of TLS and airborne (also UAV) LiDAR data [140,192], the highest z-coordinate (height is derived by the highest value of the return) [51], the vertical distance between the highest point and stem base at the ground (the vertical distance is measured from the base of the stem to the highest point on a segmented point cloud) [30] and cylinder fitting (a cylinder is fitted on a segmented point cloud and the height is denoted as the highest point in a vertical cylinder) [58,145]. While they are also not without errors, these techniques have neither been developed, adjusted nor evaluated for savannas. Known problems, e.g., uncertainties from treetop shading [86], are especially common in the riparian zones of savanna ecosystems.
The extraction of LAI using TLS point clouds is not very common across all biomes [22] including the savanna. Calders et al. [79] argue that caution is needed when using LAI estimates and the variable that is measured from ground-based sensors is effective Plant Area Index (ePAI) and effective Wood Area Index (eWAI). They recommend that TLS measurements are ideal for estimation of gap fraction, ePAI and eWAI as compared to instruments that are dependent on certain illumination conditions [79]. Gap fraction is an indirect way of estimating LAI and TLS is recommended for the estimation of gap fraction to derive ePAI and eWAI [79]. It has been used successfully in the past to estimate LAI [193]. TLS intensity data can also be used to compute LAI. They allow for differentiating between photosynthetically active (green) from non-active (woody) vegetation parts [39,54]. However, they can also be compromised by instrumental, atmospheric and object properties as well as the scanning geometry [82,129]. In order to reliably make use of TLS intensity data for vegetation applications, conversion of instrumental intensity into the proportional or equal target reflectance is required [129,135].
While tree crown attributes have been successfully derived from TLS point clouds, more research needs to be directed towards crown change and shrub parameters. With regard to the former, the method by Olivier et al. [50] needs to be tested in the savanna biome. Vierling et al. [170] suggested the use of vantage points, high cliffs or tall tripods in order to achieve steep viewing zenith angles. These enable the acquisition of enough returns from both vegetation and ground. Following this procedure, reliable construction of DEMs and characterization of vegetation structure is ensured. Other ways to capture more accurate canopy details are the acquisition of full-waveform TLS data [170], the separation of trees and shrubs on the basis of height thresholds and noise filtering [92,148,170] and also separation through the application of reflectance thresholds and LiDAR return deviations [69].
Various reconstruction algorithms are available to extract plant volume from TLS data [48,54,96,101]. A limitation common to many of these techniques is that they rely on simple geometries (e.g., cylinders) to model vegetation components. This usually leads to an overestimation of volume due to the tapering of branches and the complexity of the tree architecture [176]. Another challenge lies in the exact evaluation of TLS-based volume estimates. True validation requires destructive sampling which is seldomly possible and desired [176]. Despite these limitations, volume reconstructions from TLS point clouds are key to improving tree allometries, non-destructively estimating and monitoring biomass change as well as assessing structural gains and losses in savanna ecosystems [30,175].
AGB is a key parameter in the study of vegetation structure and crucial indicator of terrestrial carbon pools and productivity [140,190]. Accordingly, it is also a popular secondary vegetation attribute in the TLS literature. Volume estimates form the basis of AGB assessments with TLS data, which are less prone to errors when compared to the simple use of allometric equations [40,176]. QSMs are most frequently employed for estimating AGB from TLS point clouds. However, reconstruction quality still suffers from certain errors and uncertainties. These are related to the modelling of complex tree crowns and smaller branches, the optimal use of wood density values (which vary both within and between species and regions), the use of simple reconstruction geometries such as cylinders, the separation of leaf and woody components as well as the validation of AGB values [71,176]. Despite these limitations, volume-based AGB estimates do not require information on DBH and show better agreement with reference data. As a nondestructive proxy for estimation of AGB, they should be further explored in the savanna context [40,176].
Using TLS point clouds, branch level parameters from a much larger percentage of a whole tree can be extracted [194]. Combined with other tree attributes, they hold the potential to calculate whole tree carbon and water use [194]. Especially reconstruction algorithms like QSMs [177] have enabled their retrieval. To date, there are very few studies on this topic and none of them focused on savannas. QSMs have been reported to yield accurate results when modelling thick branches [195]. Accuracies usually drop for smaller branches, particularly in cases where leaf-on scans are employed [195]. In order to improve QSMs with regard to branch parameter retrieval, they should be able to correctly separate the point cloud into leaf and woody components [71].
Shrub volume and AGB has also been estimated using TLS-based reconstruction algorithms. Greaves et al. [142] employed volumetric surface differencing and voxel counting in the arctic tundra whereas Olsoy et al. [54] used voxel-based and 3D convex hull techniques to assess the volume of shrubs. The existing methods hold the potential to be successfully transferred and adjusted to savanna ecosystems. This would allow for quantifying shrub layer biomass and help making more accurate AGB assessments overally. Moreover, these integrated biomass estimates could be used as calibration and validation reference data for mapping and monitoring savannas at larger spatial scales [142].

Future Outlook
The accurate extraction of vegetation parameters from TLS data is a challenging task. This review has shown that there are still a lot of research gaps that need to be addressed. This is especially true for savannas, which are in many ways much different from those biomes that have so far gained more attention in the literature. Savannas are heterogeneous and dynamic ecosystems that feature unique and complex mixtures of trees, shrubs and grasses. This requires novel developments, (re-) adjustments as well as extensive tests and experiments when it comes to TLS algorithms and applications.
Savannas are regularly subject to disturbances from fire, herbivory, droughts and humans. These disturbances lead to vegetation structural changes (including the loss of large trees [66,196] that can affect savanna ecological functioning [196]. As a future avenue for mapping and monitoring savanna vegetation, the acquisition and analysis of multitemporal TLS point clouds should be considered. While research on this subject is still in its infancy (e.g., [49,50,115]), there is no doubt that it holds a large potential for characterizing and understanding savanna dynamics [70], including phenological processes (i.e., leaf-on vs. leaf-off conditions) [54]. Among others, this is because of the non-destructive nature of TLS [49,50] and its ability to repeatedly capture 3D vegetation information with great detail [58]. Levick and Asner et al. [196] quantified the loss of large savanna trees and observed declines of more than 30% for some sites. However, since they were using ALS, below-canopy treefall and canopy-related losses (e.g., branch breakages) could not detected [196]. Such limitations can be addressed by acquiring and investigating time series of TLS scans. By developing suitable change detection algorithms, multi-temporal TLS will enable accurate and regular accounting of carbon losses and gains in savannas.
To date, woody AGB mapping in the savanna has mainly been focusing on tree biomass. Despite being a key component of savannas, shrubs have not been considered in this regard. Future research should therefore focus on an accurate and more holistic assessment of woody AGB in savannas, including trees, small understory trees and shrubs [65]. One pathway to achieve this would be a combination of TLS and airborne LiDAR data. TLS is well-suited to characterize understory vegetation structure in terms of both trees and shrubs [64,91]. Especially regarding the latter, existing algorithms that have initially been developed for sagebrush and arctic shrubs [54,110,142] could be tested and refined. Airborne (including UAV) LiDAR offers 3D point clouds with a bird's eye perspective of the savanna. While it has been found to be of limited use in shrub-dominated ecosystems [170], it is superior for tree height and canopy structure mapping [27]. By combining TLS and airborne LiDAR data, synergies between both technologies are exploited while their individual limitations can be overcome. To increase spatial coverage and to create wall-to-wall AGB maps, optical (e.g., Landsat and Sentinel-2) and SAR (e.g., Sentinel-1, ALOS PALSAR-1/2, BIOMASS) satellite time series should be integrated into such a monitoring scheme.
From a technical perspective, TLS-based assessments of volume and AGB have not adequately taken into account the different types of woody vegetation in the savanna. An opportunity to improve them would be the separation of TLS point clouds into individual trees and shrubs. Afterwards, specific 3D models tailored to each savanna life form as well as dedicated noise filtering algorithms could be applied to yield more accurate reconstruction results [64,92,170]. For instance, QSMs could be employed to extract volume information from tree point clouds while convex hulls or voxels could be used in the case of shrub point clouds. To this end, methods exist that have been tested on trees in temperate [37], boreal [96,175] and tropical forests [101,151] as well as shrubs in the temperate regions [54,181]. Future studies in the savanna need to transfer, train and validate these techniques.
TLS intensity data have already been demonstrated to hold potential for vegetation analyses in a few studies. However, only limited overall evidence can be found in the literature so that a number of unresolved scientific questions still remain. In particular, more research is needed on the improved retrieval of the LAI with TLS intensity [80,150]. Among others, this could be achieved by a better separation of leaf and wood material [176]. Other challenging tasks based on TLS reflectance data comprise the study of seasonal changes (i.e., leaf-on vs. leaf-off vegetation properties) [54] and leaf biochemical parameters to understand the photosynthetic capacity of savanna vegetation [27].

Conclusions
3D data from TLS can make a vital contribution to mapping the current state of savanna vegetation, monitoring and quantifying its dynamics over space and time and, in this way, provide a better understanding of the underlying ecological factors and processes as the very basis for sustainable savanna management and long-term conservation. This study reviewed the scientific literature on the use of TLS for extracting vegetation parameters with a special geographic focus on savannas. The current state of the art was presented and methods relevant for TLS research in the savanna biome were identified and discussed. To achieve this, a comprehensive quantitative and qualitative meta-analysis of 113 research articles was conducted using 18 attributes. It was found that only few studies have dealt with TLS data for vegetation analyses in the savanna. Among others, research gaps exist with respect to a better separation of leaf and woody components in TLS point clouds, the accurate 3D reconstruction of savanna vegetation volume and biomass, more holistic assessments of woody AGB by considering both trees and shrubs as well as the application of TLS time series data for multi-temporal assessments of vegetation structural change, including studies quantifying seasonal differences in vegetation dry and green biomass. Future research should thus concentrate on extending previous experiments to savannas as well as developing novel information extraction routines for these.

Conflicts of Interest:
The authors declare no conflict of interest.