The Effects of the Exercise Intervention on Fundamental Movement Skills in Children with Attention Deﬁcit Hyperactivity Disorder and/or Autism Spectrum Disorder: A Meta-Analysis

: To explore the effect of exercise intervention on fundamental movement skills (FMS) of children with attention deﬁcit hyperactivity disorder (ADHD) and/or autism spectrum disorder (ASD). Following the principle of PICOS, randomized controlled trials of the effect of exercise intervention on the FMS of ADHD/ASD children were searched. A total of 12 articles and 396 participants were included. Review Manager5.4 and Stata16.0 software were used to process and analyze the data. The results revealed that (1) exercise intervention can improve the gross motor skills of children with ADHD/ASD ( p < 0.00001). Aquatic therapy (SMD = 56.54, 95% CI = 38.83–74.25) has a better effect on stability skills, and FMS intervention (SMD = 17.58, 95% CI = 1.78–33.38) has a better effect on locomotor skills and object control skills. (2) Exercise intervention can improve the ﬁne motor skills of children with ADHD/ASD ( p = 0.001). Table tennis exercise (SMD = 9.91, 95% CI = 0.23–19.59) and horse-riding program (SMD = 9.50, 95% CI = 5.20–13.80) have better effects on ﬁne manual control and hand–eye coordination. (3) The closed-skill intervention for 60 min each time, twice a week, for at least 12 weeks had the best effect on the improvement in the FMS in children with ADHD/ASD ( p < 0.00001). Exercise intervention may effectively improve FMS in children with ADHD/ASD. Intervention form, time, frequency, and duration are important moderator variables that positively impact the FMS of children with ADHD/ASD.


Introduction
Attention deficit hyperactivity disorder (ADHD) and autism spectrum disorder (ASD) are the most common neurodevelopmental disorders in children, which have a significant impact on children's physical and mental health [1]. Children with ADHD are characterized by inattention, hyperactivity, impulsivity, difficulty in controlling behavior, or such comprehensive symptoms, while children with ASD have apparent deficits in social communication skills and repetitive stereotyped behavior patterns [2,3]. Despite significant differences in the core symptoms between children with ADHD and ASD, clinical studies have supported the similarity of the behavioral disorders, and the findings suggest that the two types of children not only have highly shared heritability, but also represent the performance of the same endophenotype and can be classified into the same diagnostic entity [4,5]. While evidence of atypical brain development suggests that children with both ADHD and ASD may be affected by cerebral laterality and develop severe motor coordination deficits, it is worth noting that children with developmental coordination disorder (DCD) are the most common co-occurring disorder in the above two types of children, but the underlying mechanism evidence is insufficient, so only ADHD and ASD children are discussed and studied [6].

Inclusion and Exclusion Criteria
The PICOS principle is a structured method for searching the literature and is the basis for formulating the eligibility criteria for a meta-analysis. The PRISMA statement divides the PICOS principle into five main components: participants, interventions, comparisons, outcomes, and study design [22]. Following the PICOS principle, the inclusion criteria were: (1) the participants were children with ADHD and/or ASD; (2) the experimental group received structured exercise intervention, while the control group did not receive structured exercise intervention; (3) the outcome indicators were rating scales related to FMS of ADHD/ASD children; (4) the study design was a randomized controlled trial (RCT); Sustainability 2023, 15, 5206 3 of 13 and (5) no medication or other exercise interventions were used in the experimental group and the control group. The exclusion criteria were: (1) neither children with ADHD nor children with ASD; (2) the experimental group was an unstructured exercise intervention or a mixed intervention; (3) no outcome data was shown; (4) non-RCTs; and (5) conference papers, reviews, or duplicate published studies.

Data Extraction
To improve the quality of literature information extraction, two researchers independently performed information extraction. The following information was included in the design of the data extraction form: the first author, year of publication, diagnostic standard, subject type, data from the experimental and control groups (sample size and age), exercise intervention (form, time, frequency, and duration), assessment scales, and outcome indicators.

Risk of Bias Assessment
Risk of bias assessment for included studies is an important part of the literature quality assessment. The Cochrane risk of bias assessment tool [23] is the most widely used in randomized controlled trials, mainly including seven items: random sequence generation, allocation concealment, blinding of participants and personnel, blinding of outcomes assessment, incomplete outcome data, selective reporting, and other biases. Each item was judged to be low risk of bias, unclear risk of bias, and high risk of bias. The risk of bias was assessed by two researchers independently, and if there was any disagreement, this was resolved by mutual agreement or determined by a third reviewer.

Data Analysis and Synthesis
Review Manager 5.4 software was used for the heterogeneity test and subgroup analysis, and Stata 16.0 software was used for the sensitivity analysis and publication bias test. Continuous outcomes were expressed as the standardized mean differences (SMDs) with 95% confidence intervals (CIs).
Heterogeneity was tested by p value and I 2 . When p > 0.10 and I 2 < 50%, it will be considered that there was no heterogeneity and a fixed-effects model was used for metaanalysis. When p < 0.10 and I 2 > 50%, it was considered that there was heterogeneity and a random-effects model was used for meta-analysis. Subgroup analysis was conducted for categorical variables such as the type, time, frequency, and duration. Sensitivity analysis was used to test the stability of the meta-analysis results by excluding individual studies one by one. Publication bias was identified by plotting funnel plots and Egger linear regression.

Study Characteristics
The flowchart of the literature selection process is shown in Figure 1. A total of 1056 articles were initially searched through the database and other channels (Google Scholar). Endnote software was used to remove 228 duplicate articles, and 781 articles were excluded after reading the title and abstract. In addition, 35 articles were excluded for the following reasons: (1) non-RCTs; (2) mixed intervention; (3) no outcome data was shown, and 12 articles (six ADHD, six ASD) were finally included in the meta-analysis. Table 1 shows the basic information of the 12 included articles including the references, diagnostic standard, characteristics of the children, exercise interventions (form, time, frequency, and duration), assessment scales, and outcome indicators (gross and fine motor skills). A total of 379 children with ADHD and ASD were included, 190 in the experimental group and 189 in the control group. The sample sizes for both the experimental and control groups were 5-42 individuals, aged 3-16 years. The exercise intervention time was 35-90 min, the frequency was 1-5 times/week, and the duration was 4-20 weeks.  Table 1 shows the basic information of the 12 included articles including the refer ences, diagnostic standard, characteristics of the children, exercise interventions (form time, frequency, and duration), assessment scales, and outcome indicators (gross and fin motor skills). A total of 379 children with ADHD and ASD were included, 190 in the ex perimental group and 189 in the control group. The sample sizes for both the experimenta and control groups were 5-42 individuals, aged 3-16 years. The exercise intervention tim was 35-90 min, the frequency was 1-5 times/week, and the duration was 4-20 weeks.

Risk of Bias
The results of the bias risk assessment are shown in Figure 2. The risk of selective reporting and other biases in 12 articles was low risk of bias, the risk of blinding bias for allocation concealment and outcome assessment was unclear, and the blinding of the participants and personnel was high risk of bias. In addition, the risk of bias in random sequence generation was unclear in three articles [24,32,34], and only four articles [25,30,32,35] mentioned complete outcome indicators. In summary, the 12 articles included may have the risk of bias.

Risk of Bias
The results of the bias risk assessment are shown in Figure 2. The risk of selective reporting and other biases in 12 articles was low risk of bias, the risk of blinding bias for allocation concealment and outcome assessment was unclear, and the blinding of the participants and personnel was high risk of bias. In addition, the risk of bias in random sequence generation was unclear in three articles [24,32,34], and only four articles [25,30,32,35] mentioned complete outcome indicators. In summary, the 12 articles included may have the risk of bias.

The Effect of Exercise Intervention on Gross Motor Skills of ADHD/ASD Children
There were 12 studies on the effect of exercise intervention on the gross motor skills of ADHD/ASD children. The total sample size of the experimental group was 190, and the total sample size of the control group was 189. As shown in Figure 3, due to the heterogeneity among the study results (p < 0.00001, I 2 = 79%), a random effect model was used for the meta-analysis. The results showed that there was a significant difference between the experimental group and the control group (p < 0.00001), indicating that exercise intervention may improve the gross motor skills in ADHD/ASD children (SMD = 5.93, 95% CI = 4.05-7.82). Among them, Feng's aquatic therapy (SMD = 56.54, 95% CI = 38.83-74.25) was more effective in improving the stability skills of the ADHD/ASD children, and Dong's FMS intervention (SMD = 17.58, 95% CI = 1.78-33.38) was more effective in improving the locomotor skills and object control skills of both types of children.

The Effect of Exercise Intervention on Gross Motor Skills of ADHD/ASD Children
There were 12 studies on the effect of exercise intervention on the gross motor skills of ADHD/ASD children. The total sample size of the experimental group was 190, and the total sample size of the control group was 189. As shown in Figure 3, due to the heterogeneity among the study results (p < 0.00001, I 2 = 79%), a random effect model was used for the meta-analysis. The results showed that there was a significant difference between the experimental group and the control group (p < 0.00001), indicating that exercise intervention may improve the gross motor skills in ADHD/ASD children (SMD = 5.93, 95% CI = 4.05-7.82). Among them, Feng's aquatic therapy (SMD = 56.54, 95% CI = 38.83-74.25) was more effective in improving the stability skills of the ADHD/ASD children, and Dong's FMS intervention (SMD = 17.58, 95% CI = 1.78-33.38) was more effective in improving the locomotor skills and object control skills of both types of children. There were four studies on the effect of exercise intervention on the fine motor skills of ADHD/ASD children. The total sample size of the experimental group was 69, and the total sample size of the control group was 69. As shown in Figure 4, due to the heteroge-

The Effect of Exercise Intervention on Fine Motor Skills of ADHD/ASD Children
There were four studies on the effect of exercise intervention on the fine motor skills of ADHD/ASD children. The total sample size of the experimental group was 69, and the total sample size of the control group was 69. As shown in Figure 4, due to the heterogeneity among the study results (p = 0.05, I 2 = 62%), a random effect model was used for the meta-analysis. The results showed that there was a significant difference between the experimental group and the control group (p = 0.001), indicating that exercise intervention might improve the fine skills of ADHD/ASD children (SMD = 6.32, 95% CI = 2.42-10.21). Among them, Pan's table tennis exercise (SMD = 9.91, 95% CI = 0. 23-19.59) and horse-riding program (SMD = 9.50, 95% CI = 5.20-13.80) had better effects on the fine manual control and hand-eye coordination in both types of children.

The Effect of Exercise Intervention on Fine Motor Skills of ADHD/ASD Children
There were four studies on the effect of exercise intervention on the fine motor skills of ADHD/ASD children. The total sample size of the experimental group was 69, and the total sample size of the control group was 69. As shown in Figure 4, due to the heterogeneity among the study results (p = 0.05, I 2 = 62%), a random effect model was used for the meta-analysis. The results showed that there was a significant difference between the experimental group and the control group (p = 0.001), indicating that exercise intervention might improve the fine skills of ADHD/ASD children (SMD = 6.32, 95% CI = 2.42-10.21). Among them, Pan's table tennis exercise (SMD = 9.91, 95% CI = 0. 23-19.59) and horseriding program (SMD = 9.50, 95% CI = 5.20-13.80) had better effects on the fine manual control and hand-eye coordination in both types of children.

Subgroup Analysis
To further explore potential sources of heterogeneity, we analyzed the FMS of the subgroups according to the moderator variables (see Table 2), suggesting that the source of heterogeneity may be related to the intervention form, time, frequency, and duration. Among them, the closed-skill intervention for 60 min each time, twice a week, for at least 12 weeks, had the best effect on the improvement in the FMS in children with ADHD/ASD (p < 0.00001).

Subgroup Analysis
To further explore potential sources of heterogeneity, we analyzed the FMS of the subgroups according to the moderator variables (see Table 2), suggesting that the source of heterogeneity may be related to the intervention form, time, frequency, and duration. Among them, the closed-skill intervention for 60 min each time, twice a week, for at least 12 weeks, had the best effect on the improvement in the FMS in children with ADHD/ASD (p < 0.00001). Notes: Because the "Frequency" and "Duration" data in the Kosari study were unclear, they were not included in the study.

Sensitivity Analysis
In the included studies, samples of gross motor skills were mainly distributed on the left side, and samples of fine motor skills were distributed on the right side. By excluding individual studies one by one, a sensitivity analysis was performed to test the stability of the overall experimental results. As shown in Figure 5, the effect sizes of both sides of the sample after excluding each study were within the upper and lower limits of the 95% confidence interval. Wuang's study had a high degree of scatter, but it had little effect on the overall experimental results, which indicates that the population effect size of this study was not affected by one-tailed bias. The results showed that in the study on the FMS of children with ADHD and ASD affected by exercise intervention, there was no significant change in the experimental results, suggesting that the results were relatively stable and reliable.

Publication Bias
Publication bias was first tested by plotting a funnel plot. The scatter points in Figure  6 represent the studies included in the meta-analysis. Most of the scatter points were located in the middle of the funnel and were asymmetrical on the left and right sides of the vertical line. There may be publication bias in the preliminary analysis. Next, Egger linear regression was used to further test for publication bias. As shown in Figure 7, the left vertical line is the confidence interval corresponding to the intercept α, which had a 95% CI (−0.29, 9.12), p = 0.063 > 0.05, indicating that there was no publication bias in the included studies. In summary, the findings indicated that this study was less affected by publication bias and that the findings were reliable.

Publication Bias
Publication bias was first tested by plotting a funnel plot. The scatter points in Figure 6 represent the studies included in the meta-analysis. Most of the scatter points were located in the middle of the funnel and were asymmetrical on the left and right sides of the vertical line. There may be publication bias in the preliminary analysis. Next, Egger linear regression was used to further test for publication bias. As shown in Figure 7, the left vertical line is the confidence interval corresponding to the intercept α, which had a 95% CI (−0.29, 9.12), p = 0.063 > 0.05, indicating that there was no publication bias in the included studies. In summary, the findings indicated that this study was less affected by publication bias and that the findings were reliable. cated in the middle of the funnel and were asymmetrical on the left and right sides of the vertical line. There may be publication bias in the preliminary analysis. Next, Egger linear regression was used to further test for publication bias. As shown in Figure 7, the left vertical line is the confidence interval corresponding to the intercept α, which had a 95% CI (−0. 29, 9.12), p = 0.063 > 0.05, indicating that there was no publication bias in the included studies. In summary, the findings indicated that this study was less affected by publication bias and that the findings were reliable.

Exercise Intervention Has a Significant Effect on Gross Motor Skills of ADHD/ASD Children
Meta-analysis studies have shown that exercise intervention can improve the gross motor skills in children with ADHD/ASD, which is consistent with the findings of Ruggeri et al. [12,36]. The underlying mechanism by which exercise intervention induces improvements in gross motor skills may be related to neuroanatomy [9,37]. On one hand, exercise intervention can cause a series of biological reactions in the muscles and organs, promoting neurogenerative, neuroadaptive, and neuroprotective processes to favorably affect the structural development and functional neurocognitive development of the brain primarily through the regulation of the brain's neural circuitry, mediation of executive function, and lasting impact on the developmental trajectory of gross motor skills in children with ADHD/ASD [38,39]. On the other hand, exercise intervention may help the prefrontal cortex of the brain to formulate and improve perception-motor related skill tasks, while the prefrontal cortex of the brain can get away to focus on cognitive and adaptive functions, allowing for the gradual automation of gross motor skills [40].
Among the gross motor skill interventions, six types [24,[26][27][28][29][30][31][33][34][35] of exercise interventions could improve the locomotor skills of ADHD/ASD children, and only two types [24,30,31] could improve the object control skills for both types of children. Of these, the FMS intervention [30,31] and the table tennis exercise [24] had the potential to improve both the locomotor skills and object control skills in both types of children, but the enhancing effect of the FMS intervention was better. Further analysis found that the FMS intervention could not only provide a goal-oriented, structured, and progressive guidance strategy, positively change the functional connection of the brain's neural network and have an impact on the brain's neurogenesis, adaptation, and protection [38,41], but in particular, it created the best stimulating environment in the learning situation and helped children with ADHD/ASD to create a positive self-perception and experience [30]. In addition, six types [25][26][27][28][32][33][34][35] of exercise interventions could improve the stability skills in children with ADHD/ASD, but aquatic therapy had the best improvement effect. Aquatic therapy mainly applies the principles of hydrodynamics to provide multiple sen-

Exercise Intervention Has a Significant Effect on Gross Motor Skills of ADHD/ASD Children
Meta-analysis studies have shown that exercise intervention can improve the gross motor skills in children with ADHD/ASD, which is consistent with the findings of Ruggeri et al. [12,36]. The underlying mechanism by which exercise intervention induces improvements in gross motor skills may be related to neuroanatomy [9,37]. On one hand, exercise intervention can cause a series of biological reactions in the muscles and organs, promoting neurogenerative, neuroadaptive, and neuroprotective processes to favorably affect the structural development and functional neurocognitive development of the brain primarily through the regulation of the brain's neural circuitry, mediation of executive function, and lasting impact on the developmental trajectory of gross motor skills in children with ADHD/ASD [38,39]. On the other hand, exercise intervention may help the prefrontal cortex of the brain to formulate and improve perception-motor related skill tasks, while the prefrontal cortex of the brain can get away to focus on cognitive and adaptive functions, allowing for the gradual automation of gross motor skills [40].
Among the gross motor skill interventions, six types [24,[26][27][28][29][30][31][33][34][35] of exercise interventions could improve the locomotor skills of ADHD/ASD children, and only two types [24,30,31] could improve the object control skills for both types of children. Of these, the FMS intervention [30,31] and the table tennis exercise [24] had the potential to improve both the locomotor skills and object control skills in both types of children, but the enhancing effect of the FMS intervention was better. Further analysis found that the FMS intervention could not only provide a goal-oriented, structured, and progressive guidance strategy, positively change the functional connection of the brain's neural network and have an impact on the brain's neurogenesis, adaptation, and protection [38,41], but in particular, it created the best stimulating environment in the learning situation and helped children with ADHD/ASD to create a positive self-perception and experience [30]. In addition, six types [25][26][27][28][32][33][34][35] of exercise interventions could improve the stability skills in children with ADHD/ASD, but aquatic therapy had the best improvement effect. Aquatic therapy mainly applies the principles of hydrodynamics to provide multiple sensory stimulations through water temperature, weight reduction, and vestibular input to promote gross motor skill execution [42].

Exercise Intervention Has a Significant Effect on Fine Motor Skills of ADHD/ASD Children
The findings that exercise intervention improves the fine motor skills in children with ADHD/ASD are not consistent with the results of Zhang et al. [1]. These differences may be due to the sample size, the form and intensity of the intervention, the tactile sensitivity, and the overall lack of social imitation being different [43]. It has been shown that exercise intervention may not only promote the brain to make connections in visual feedback and motor commands, but also form connectivity with both the visual network and the dorsomedial motor region, and most likely through visual imitation to improve the hand-eye coordination ability and upper limb motor skills in children with ADHD/ASD. It may also promote central nervous system plasticity and increase neurotransmitter secretion, increasing the level of brain arousal, and thereby improving executive function and enhancing the fine manual control of both types of children [44,45].
Among the studies included in the meta-analysis, only four studies [26,27,33,35] focused on the effect of exercise intervention on the fine motor skills of children with ADHD/ASD. The table tennis exercise and horse-riding program were important interventions to improve their fine motor skills, but the specific exercise patterns differed. The successful performance of table tennis exercises mainly depends on visual perception and executive function, that is, accurately predicting the spatial trajectory and arrival time of the table tennis ball, and immediately making the selection and execution of the ball response. Table tennis exercise is based on the principle of constraint-induced exercise therapy, which guides children with ADHD/ASD to perform repetitive exercises, conceal motor potential through different strategies and tasks, and promote hand-eye coordination, fine manual control, and executive function of both types of children [27,33]. Through active vision and somatosensory control, the horse-riding program coordinated the connection between vision and the limbs and performed the movements of the limbs correctly. ADHD/ASD children are required to grasp the handle of the horse-riding machine during the horseriding program, and alter the posture and speed of their upper limbs from time to time according to the visual information and trunk movement, improving hand-eye coordination and fine manual control [26,35]. Currently, the fine motor skills of most children with ADHD/ASD show structural defects and are lower than the level of children of the same age [5,46]. Therefore, it is hoped that more appropriate exercise interventions can be used to improve the fine motor skills of both types of children.

Effects of Moderator Variables in Exercise Intervention on FMS in ADHD/ASD Children
The results of this study show that although both open-skill and closed-skill can improve FMS in children with ADHD/ASD, the improvement effect of closed-skill was better. The motor environment is considered to be the main sign that distinguishes openskill from closed-skill. Closed-skill (running, cycling, swimming, etc.) are performed in a relatively consistent, controllable, and self-regulating environment; open-skill (table tennis, football, basketball, etc.) refer to responding in an environment that is dynamic, unpredictable, and influenced by external factors. In general, open-skill interventions tend to be more complex [47,48]. The analysis of this study demonstrates that children with ADHD/ASD have defects in complex tasks, adaptive behavior, and motor development, and that performance on the FMS is significantly poorer than that of children of the same age. Therefore, simple, regular, and predictable closed-skill are more suitable for the development of FMS of both types of children [49,50].
In addition to the form of intervention, the FMS of ADHD/ASD children was also affected by the time, frequency, and duration of the intervention, which is consistent with previous meta-analysis studies [16]. This study found that an exercise intervention of 60 min each time, twice a week, for at least 12 weeks was the most beneficial for improving the FMS in children with ADHD/ASD. In terms of intervention time, each 60-min improvement effect was the best. For example, tai chi training [51] and the FMS intervention [9] implemented a 60-min structured intervention program to effectively improve the children's movements and skills. With regard to the frequency of intervention, exercise intervention once or twice a week may improve FMS in children with ADHD/ASD, and the improvement effect was better twice a week, which is consistent with the aquatic therapy of Pan [52]. Exercise intervention three times or more per week had no significant effect on improving the FMS of the two types of children, indicating that intensive exercise intervention may not be suitable for the development of both types of children, probably because the development of FMS of both types of children lags behind that of normal children, and the frequency of intervention should be consistent with their actual developmental abilities [29,34]. In terms of intervention duration, the improvement effect is better for 12 weeks or more. Long-term exercise intervention may increase the brain growth factors in children with ADHD/ASD, promote brain activity, improve cognitive ability, and enhance the practice effect of the FMS [38]. Lourenço et al. pointed out that exercise intervention lasting at least 12 weeks may stimulate the long-term attention and FMS proficiency of the two types of children to a greater extent [53,54]. In conclusion, to maximize the benefits of exercise interventions, the potential effect of moderator variables should be considered.

Conclusions
The results of the meta-analysis showed that exercise intervention could improve the gross and fine motor skills in children with ADHD/ASD; the closed-skill intervention for 60 min each time, twice a week, for at least 12 weeks had the best effect on the improvement in the FMS in both types of children. This study provides exercise intervention ideas for medical institutions, occupational therapists, special education teachers, physical education teachers, and parents of children with ADHD/ASD, and provides a basis for future research into the improvement of the FMS of both types of children.

Limitations and Future Research
One important limitation of this study was the small sample size of children with ADHD/ASD included in the study, which may have impacted the statistical analysis and limited the generalizability of the results. Another limitation of this study is the lack of follow-up studies analyzed from a longitudinal perspective, which makes it difficult to scientifically reflect the long-term timeliness of exercise intervention on the FMS of both types of children. Moreover, this study mainly focused on school-based interventions, lacked an exploration of family and community-based interventions, and ignored the effect of integration across different modes of intervention. However, there were still some shortcomings in this study. In terms of the neural mechanism, although children with ADHD/ASD have abnormalities in brain structure and function, there is still a lack of neuroanatomical evidence for the intrinsic connection between exercise intervention and the brain. In terms of the subgroup analysis, the discussion of moderator variables was not sufficiently comprehensive. Currently, only the intervention form, time, frequency, and duration have been studied.
Based on the above discussion, several suggestions are offered for exercise intervention to improve the FMS of children with ADHD/ASD. First, to further expand the sample size of randomized controlled trials on the effect of exercise intervention on the FMS of children with ADHD/ASD to ensure the applicability of the research results. Second, pay attention to whether the long-term longitudinal follow-up study has a positive effect on the FMS for both types of children, and provide ideas and directions for the experimental design of later exercise interventions. Third, to build a cooperative exercise intervention model for families, schools, and communities, and to explore the effect of the comprehensive intervention model on the FMS of both types of children. Fourth, to continue to explore the brain structure and functional mechanisms in both types of children. As above-mentioned, children with ADHD/ASD have abnormal cerebral lateralization, but how to integrate the functions of the two sides of the brain through exercise intervention to improve the FMS of both types of children has yet to be explored. Finally, by considering the adjusting variable of increasing exercise intensity, the effects of different intensities of exercise on the FMS of both types of children were compared.

Data Availability Statement:
No new data were created or analyzed in this study. Data sharing is not applicable to this article.