Micro-and Nano-Bubbles Enhanced the Treatment of an Urban Black-Odor River

: Black-odor water is prevalent in southeastern coastal regions of China, compromising both the aquatic ecosystem and urban aesthetics. Micro-and nano-bubbles (MNBs) aeration, identiﬁed as an innovative approach, offers potential improvements in water ecological function. This study introduces and implements an MNBs technique to rehabilitate an urban black-odor river. Results indicate that MNBs aeration achieved a signiﬁcantly higher increment rate of dissolved oxygen (89.4%) and higher removal efﬁciencies of biological oxygen demand (54.4%), chemical oxygen demand (39.0%), ammonia nitrogen (63.2%), total phosphorus (28.0%) and dimethyl trisulﬁde (100%) in the water compared to conventional blast aeration. Concurrently, a 25.0% increase in the ratio of iron/aluminum-bonded phosphorus (Fe/Al-P) to total sediment phosphorus effectively curtailed endogenous phosphorus release. Additionally, MNBs aeration markedly reduced plankton biomass, suggesting direct removal by MNBs. This enhanced performance is attributable to the improved oxygen mass transfer coefﬁcient and oxygenation capacity, fostering more efﬁcient pollutants. Furthermore, MNBs signiﬁcantly encouraged the growth of aerobic microorganisms (e.g., Actinobacteria , Firmicutes and Myxococcota ) in the sediment, bolstering the water’s self-puriﬁcation ability. Consequently, this study validates MNBs as a highly promising solution for treating black odorous water bodies.


Introduction
The rapid urbanization and industrialization have significantly compromised water environments [1].A considerable influx of pollutants, such as those from domestic sewage and industrial effluents, results in elevated levels of chemical oxygen demand (COD), suspended solids, nitrogen, phosphorus and other contaminants in aquatic systems [2][3][4].This accumulation of pollutants depletes oxygen in water bodies, promoting the production of blackening materials and odorous gases.Such changes not only impair the water's ecosystem structure and self-purification ability but also cause sensory discomfort to residents and mar the urban landscape [5,6].A 2016 report indicated that approximately 73% of 295 Chinese cities at the prefecture level and above were afflicted with black-odor water bodies [7].In response, the Chinese government launched the "Action Plan for Water Pollution Prevention and Control" in 2015, aiming to eliminate over 90% of black-odor rivers by 2020 and virtually all such rivers by 2030 [8].Consequently, the issue of black-odor water has emerged as a critical environmental challenge, necessitating prompt and effective restoration measures.
Over the years, aeration technology has increasingly been utilized to address blackodor water issues, owing to its notable enhancement of water self-purification [9,10].Among various methods, micro-and nano-bubbles (MNBs), characterized by their longevity, minimal buoyancy and high gas solubility, have emerged as particularly effective in wastewater treatment [11][12][13].MNBs offer two primary advantages: firstly, their larger surface area facilitates more efficient O 2 mass transfer, and the elevated vapor pressure of O 2 enhances the solubility of dissolved oxygen (DO) [14].Secondly, the collapse of MNBs generates a range of reactive oxygen species, such as superoxide anion radicals (O 2 • − ) and singlet oxygen ( 1 O 2 )), which possess strong oxidative properties conducive to pollutant degradation [15,16].Comparative studies have shown that MNBs aeration can deliver oxygen transfer efficiency more than tenfold higher and reduce oxygen decline rates by over 50% compared to macro-bubble aeration [4].Furthermore, MNBs distinctly improve the removal of total organic carbon (TOC), COD, ammonia nitrogen (NH 3 -N), Total Nitrogen (TN), total phosphorus (TP), odorous compounds (e.g., dimethyl trisulfide (DMTS) and 2-methylisotethanol (2-MIB)) and black substances (e.g., fulvic acid) and obviously expedite the microbial activities of nitrification and aerobic ammonia oxidation in polluted water bodies [4,9,10,17].For instance, MNBs aeration achieved approximately 15% higher TOC and NH 3 -N removal efficiencies compared to traditional aeration in treating livestock wastewater [10].DMTS content in black-odor water was reduced by 99% within two hours under MNBs treatment, compared to a 67% reduction over one day with blast aeration [18].In small water bodies, nearly all microorganisms in the MNBs treatment system were aerobic microbes, whereas no significant differences were noted between the percentage of aerobic microorganisms, contrasting the balanced presence of aerobic and anaerobic microorganisms in traditional macro-bubble aeration systems [17].Therefore, MNBs demonstrate a superior capability in pollutant removal relative to conventional aeration techniques.
In China, approximately 60% of the black-odor rivers are concentrated in the more developed southeastern coastal areas.Among these, Tangmenbang, situated in the Yangtze River Delta economic circle, exemplifies a typical polluted urban black-odor river, characterized by its gray-black color with a pungent odor.To address this issue, an MNBs technology, founded on the principle of gas-liquid high-speed hydraulic spinning, was developed specifically for river remediation.In the current study, the objectives were as follows: (1) to investigate the physicochemical properties of the urban black-odor river; (2) to evaluate the effects of MNBs aeration on water and sediment quality; (3) to examine the responses of plankton composition and the microbial community to MNBs aeration.

Study Area
Tangmenbang, an urban river characterized by its distinct black odor, is situated in Changzhou, Jiangsu Province, China (refer to Figure 1).This river spans 1.5 km in length and 20 meters in width.It features two sluice gates that regulate water flow, often resulting in stagnant conditions.Along its course, 31 effluent discharge points have been identified, releasing various types of waste including domestic and industrial sewage.Notably, seven of these points remain actively operational (as detailed in Supplementary Materials Figure S1).Additionally, the riverbanks are marred by the accumulation of household refuse.Direct discharges into the river from sources such as livestock breeding facilities, septic tanks, and untreated initial rainwater runoff further contribute to its complex pollution profile.

Experiment Settings and Sample Collection
Three experimental sites along the river, designated as T1, T2 and T3, were selected for study (see Figure 1).Site T1 served as control, Site T2 employed blast aerators, and Site T3 utilized MNBs generators.The blast aerators operated using a high-pressure eddy fan, providing air at 0.029 MPa and 80 m 3 /h, in conjunction with aeration discs for bubble production.MNBs aeration, based on gas-liquid high-speed hydraulic spinning, involved a gas-liquid mixing pump and a bubble generator, detailed in Supplementary Materials Section S2.The MNBs equipment operated at an outlet pressure of 0.55 MPa and an air intake of 3.5 m 3 /h.The resultant bubble size predominantly ranged from 20 to 30 µm in diameter (refer to Figure 2a) [18].A comparative analysis indicated the reoxygenation capacity of MNBs was significantly superior to blast aeration, as evidenced by the Mann-Whitney U test (p < 0.001) (see Figure 2b).

Experiment Settings and Sample Collection
Three experimental sites along the river, designated as T1, T2 and T3, were selected for study (see Figure 1).Site T1 served as control, Site T2 employed blast aerators, and Site T3 utilized MNBs generators.The blast aerators operated using a high-pressure eddy fan, providing air at 0.029 MPa and 80 m 3 /h, in conjunction with aeration discs for bubble production.MNBs aeration, based on gas-liquid high-speed hydraulic spinning, involved a gas-liquid mixing pump and a bubble generator, detailed in Supplementary Materials Section S2.The MNBs equipment operated at an outlet pressure of 0.55 MPa and an air intake of 3.5 m 3 /h.The resultant bubble size predominantly ranged from 20 to 30 µm in diameter (refer to Figure 2a) [18].A comparative analysis indicated the reoxygenation capacity of MNBs was significantly superior to blast aeration, as evidenced by the Mann-Whitney U test (p < 0.001) (see Figure 2b).

Experiment Settings and Sample Collection
Three experimental sites along the river, designated as T1, T2 and T3, were selected for study (see Figure 1).Site T1 served as control, Site T2 employed blast aerators, and Site T3 utilized MNBs generators.The blast aerators operated using a high-pressure eddy fan, providing air at 0.029 MPa and 80 m 3 /h, in conjunction with aeration discs for bubble production.MNBs aeration, based on gas-liquid high-speed hydraulic spinning, involved a gas-liquid mixing pump and a bubble generator, detailed in Supplementary Materials Section S2.The MNBs equipment operated at an outlet pressure of 0.55 MPa and an air intake of 3.5 m 3 /h.The resultant bubble size predominantly ranged from 20 to 30 µm in diameter (refer to Figure 2a) [18].A comparative analysis indicated the reoxygenation capacity of MNBs was significantly superior to blast aeration, as evidenced by the Mann-Whitney U test (p < 0.001) (see Figure 2b).

Plankton Analysis
Ten-liter water samples were sequentially filtered on-site using a No. 25 plankton net (mesh 0.064 mm) and a No. 13 plankton net (mesh 0.112 mm) to isolate plankton, followed by the addition of Lugol's solution for preservation.Plankton enumeration was performed using a calibrated microscope, adopting a methodology outlined in a prior study [25].A 0.1 mL aliquot of each sample was placed in a Sedgewick-Rafter counting chamber and examined under a microscope at either 8 × 40-fold or 10 × 40 magnification.Each sample underwent duplicate counts.In cases where the variance between the two counts exceeded 15%, a third count was conducted for accuracy.

Statistical Analysis
The Shannon-Wiener diversity index (S-W) was computed to analyze the diversity characteristics of the plankton community [27].To assess significant differences between paired or unpaired samples, statistical analyses including the Mann-Whitney U test, Kruskal-Wallis test and Fisher's exact test were utilized.Significant differences were all declared at p < 0.05.

Physicochemical Characteristics of the Urban Black-Odor River
The physicochemical properties of water in Tangmenbang River are summarized in Table 1.As per China's "Working guidelines for the treatment of urban black-odor water" [28], black-odor intensity is categorized as mild (transparency = 10-25 cm, DO = 0.2-2.0mg/L, ORP = −200-50 mV, NH 3 -N = 8.0-15.0mg/L) and severe (transparency < 10 cm, DO < 0.2 mg/L, ORP < −200 mV, NH 3 -N < 15.0 mg/L).In the current study, average transparency at Site T1, and DO and ORP across all three sites, fell within the mild black-odor parameters (10-25 cm, 0.2-2.0mg/L and −200-50 mV, respectively).However, NH 3 -N concentrations exceeded 15 mg/L, indicative of severe black-odor conditions [28].Average TN and TP concentrations were 16.75 mg/L and 1.38 mg/L, respectively, with an N:P ratio of 12.1, suggesting N and P co-limitation in the river's nutrients [29].NH 3 -N constituted 88.9% of the TN, likely due to external pollution imported.Elevated average COD (24 mg/L) and BOD 5 (8.87 mg/L) levels pointed to intensive organic pollutions [30].The presence of black FeS or metal sulfides, often a primary cause of black odor in polluted waters, was evidenced by Fe 2+ concentrations ranging from 0.29 to 0.38 mg/L in the Tangmenbang River [6,31].Additionally, volatile organic sulfur compounds (VOSCs) analysis suggested DMTS as the predominant odor-causing compound, with concentrations ranging from 2081 to 3419 ng/L.Surface sediment TOC content ranged from 6.80 to 7.00 g/kg (Table 1), with the C/N ratio exceeding 20, indicating an allochthonous origin for the organic matter [32].NH 3 -N and NO 3 -N comprised 38.3% and 1.97% of TN, respectively, pointing to potential nitrification inhibition [33].A high Fe concentration (19.6-23.0g/kg) was noted, likely due to extensive metal processing manufacturers around the river, which may significantly affect phosphorus mobility [34].The ratio of Fe/Al-bond phosphorus (Fe/Al-P) to TP was about 30%, attributed to the low ORP facilitating the reduction of Fe (III) to Fe (II) [35].Thus, some PO 4 3-associated with iron oxides and hydro-oxides, such as hydroferritite and ferritite, could be released into the pore water, resulting in internal pollution [35].

Performance of Water and Sediment after MNBs Aeration
Figure 3 shows that after 7 days of aeration (corresponding to the first sampling after aeration), the DO, OPR, NH 3 -N and transparency measurements significantly surpassed the mild black-odor standards, indicating a rapid improvement in water quality.The process of aeration progressively led to water oxygenation and an aerobic state.The average DO content increased by 89.4% for MNBs aeration and 33.3% for blast aeration, suggesting MNBs's effectiveness in enhancing dissolved oxygen levels [4].This outcome can be attributed to the MNBs providing a higher surface area for O 2 mass transfer and a higher O 2 vapor pressure to drive the partition balance [14].The rise in DO was accompanied by an increase in ORP, indicating the prevalence of an oxidizing environment.ORP increased by 75.4% for MNBs treatment and 48.2% for blast aeration.However, a massive sewage influx in October led to a notable decrease in ORP for control and blast aeration treatments, while MNBs maintained stable OPR levels.In this oxidizing environment, the enhanced activities of nitrifying bacteria accelerated the conversion of NH 3 -N into NO 3 − or NO 2 − , resulting in a reduction in NH 3 -N concentration [9].The removal efficiency for MNBs remained at 63.2%, which was 25% greater than blast aeration.A temporary elevation of NH 3 -N in summer was owing to the abundant rainfalls that carried plenty of pollutants into the river.Furthermore, water transparency improved by 5.5 cm for blast aeration and 22 cm for MNBs.The increase in DO contributed to the degradation of colored dissolved organic matter (cDOM), sulfides and blackening metallic compounds, thereby enhancing water clarity [36].The superior performance of MNBs was attributed to their higher oxygenation capability [10].At the same time, BOD 5 , COD, TN and TP in water samples showed improvement (Figure 4).MNBs aeration demonstrated significantly higher removal efficiencies for BOD 5 (54.4%) and COD (39.0%) compared to traditional pumping systems (31.9% and 16.9%, respectively).The ratio of BOD 5 to COD decreased from 0.37 to 0.20 in the case of MNBs and to 0.24 for blast aeration, indicating substantial degradation of organic matter.This degradation could result from biodegradation by the activated microbial communities or from the direct reaction with reactive oxygen species provided by MNBs collapse [10,37].Alkaline aeration converted NH 3 -N to NH 3 , aiding in the elimination of TN [38], with an observed efficiency of about 20% in this study.The MNBs-aerated system achieved a notable TP removal rate of approximately 28%, in contrast to the negligible reduction observed with blast aeration.This difference might stem from the absorption of inorganic macromolecular colloid degraded from organic compounds and metabolism of aerobic bacteria, which expedited the migration of soluble phosphate from the upper water to the sediment [9].Higher TP concentrations (570 mg/L) in sediment after MNBs aeration compared to other treatments (520 mg/L) support this finding.Furthermore, nearly all the major odor-causing substance, DMTS, was removed within a month or less.Improved oxygen supply and retention capabilities have effectively inhibited the en dogenous release of pollutants from sediments.Specifically, the removal rates of TOC (30.6%) and NH3-N (39.2%) in sediments treated with MNBs were apparently greater than those in the blast-aerated sediments (10.2% and 7.10%, respectively) (Figure 5).The de composition and transformation of organic matter in sediments primarily involve humi fication and mineralization.Enhanced activity of aerobic indigenous microorganisms in the sediment decomposes organic compounds into simpler forms, which then undergo complex polymerization reactions to form humus [39].Additionally, the mineralization process in Fe-rich sediments likely involves reactions of organic matter with hydroxide o oxide of trivalent iron to produce carbon dioxide [40].The proliferation of nitrifying bac teria also contributes to increased NH₃-N removal through nitrification.Post-MNBs treat ment, the proportion of Fe/Al-P to TP increased from approximately 30% to 55%, specu lating that MNBs improve the binding capacity (e.g., adsorption or co-precipitation) o iron(oxy)hydroxides and aluminum hydroxides to phosphorus, thereby inhibiting endog enous phosphorus release from the sediments [34].The redox potential in oxic and alka line environments favors higher concentrations of Fe/Al-P [34].
Statistically significant differences in DO, OPR, NH3-N, transparency, COD, BOD5 TN and TOC were observed between the MNBs and control groups (Kruskal-Wallis test p < 0.05).In contrast, only DO, NH3-N and TN concentrations differed significantly be tween blast aeration and control groups (Kruskal-Wallis test, p < 0.05).MNBs aeration demonstrated superior performance overall.The findings align with prior research indi cating that MNBs had a higher oxygen mass transfer coefficient and oxygenation capabil ity for the more effective removal of pollutants [4,9,10].In this experiment, the averag energy consumption was approximately 24 kWh/d in the blast aeration and 28 kWh/d in Improved oxygen supply and retention capabilities have effectively inhibited the endogenous release of pollutants from sediments.Specifically, the removal rates of TOC (30.6%) and NH 3 -N (39.2%) in sediments treated with MNBs were apparently greater than those in the blast-aerated sediments (10.2% and 7.10%, respectively) (Figure 5).The decomposition and transformation of organic matter in sediments primarily involve humification and mineralization.Enhanced activity of aerobic indigenous microorganisms in the sediment decomposes organic compounds into simpler forms, which then undergo complex polymerization reactions to form humus [39].Additionally, the mineralization process in Fe-rich sediments likely involves reactions of organic matter with hydroxide or oxide of trivalent iron to produce carbon dioxide [40].The proliferation of nitrifying bacteria also contributes to increased NH 3 -N removal through nitrification.Post-MNBs treatment, the proportion of Fe/Al-P to TP increased from approximately 30% to 55%, speculating that MNBs improve the binding capacity (e.g., adsorption or co-precipitation) of iron(oxy)hydroxides and aluminum hydroxides to phosphorus, thereby inhibiting endogenous phosphorus release from the sediments [34].The redox potential in oxic and alkaline environments favors higher concentrations of Fe/Al-P [34].
energy efficiency ratios of MNBs to blast aeration for NH3-N, BOD5, COD, TN and TP in water samples were 0.72, 0.68, 0.50, 1.03 and 0.15, respectively.MNBs and blast aeration showed similar energy consumption for the TN removal.However, MNBs exhibited better potential and economy for NH3-N, BOD5, COD and TP removal than macro bubbles.

Response of Plankton Composition in Water to MNBs Aeration
The phytoplankton community identified in water samples included Cyanophyta, Chlorophyta, Bacillariophyta, Euglenophyta, Cryptophyta, Pyrrophyta and Cryptophyta, in which the abundance of Cyanophyta, Chlorophyta and Bacillariophyta had absolute superiority.The dominant species of Cyanophyta were Planktothrix and Limnothrix, and the Chlorophyta were dominated by Chlamydomonas.Normally, the disintegration of algae was accompanied by the production of bad taste and odor, and some species even generated a toxic secondary metabolite (e.g., cyanotoxins) that was harmful to animals and humans [1].The zooplankton community identified in the current study included protozoa, rotifers, copepods and cladocerans, and it was dominated by protozoa and rotifers.The protozoa were dominated by Tintinnopsis kiangsuensis, Vorticella sp. and Leprotintinnus fluviatile, with biomasses of 0.75-3.00mg/L, 0.00-2.8mg/L and 0.06-2.76mg/L, respectively.The rotifers were dominated by Brachionus calyciflorus with a biomass of 0.06-1.63mg/L.
Monthly variations in the species and biomass of plankton were observed in Figures 6 and 7. Notably, a surge in zooplankton boom corresponded with a substantial decline in phytoplankton biomass throughout the monitoring process (Figure 6).For instance, in July, zooplankton reached their peak biomass (6.64-16.0mg/L), while phytoplankton biomass was at its lowest (0.45-1.15 mg/L).This trend likely resulted from zooplankton predation on algae.Overall, post-MNBs aeration, plankton biomass was lower compared to other treatments, suggesting MNBs's inhibitory effect on plankton.This reduction can be attributed to two factors: firstly, aeration-induced depletion of nitrogen and phosphorus in water, limiting plankton growth; and secondly, potential disruption of plankton cell structures by MNBs, leading to their rapid settling [41].On the contrary, a low S-W diversity index was recorded (Figure 7), ranging from 0.24 to 1.25 for phytoplankton and 0.00 to 0.70 for zooplankton.This indicates a relatively simple community structure of plankton, with minimal inter-species competition contributing to increased community instability [42].Statistically significant differences in DO, OPR, NH 3 -N, transparency, COD, BOD 5 , TN and TOC were observed between the MNBs and control groups (Kruskal-Wallis test, p < 0.05).In contrast, only DO, NH 3 -N and TN concentrations differed significantly between blast aeration and control groups (Kruskal-Wallis test, p < 0.05).MNBs aeration demonstrated superior performance overall.The findings align with prior research indicating that MNBs had a higher oxygen mass transfer coefficient and oxygenation capability for the more effective removal of pollutants [4,9,10].In this experiment, the average energy consumption was approximately 24 kWh/d in the blast aeration and 28 kWh/d in the MNBs aeration.Although MNBs aeration consumed more energy than blast aeration at the same working time, it achieved higher oxygen transfer efficiency (Figure 2b).When considering the daily average energy consumption per gram of pollutant removed, the energy efficiency ratios of MNBs to blast aeration for NH 3 -N, BOD 5 , COD, TN and TP in water samples were 0.72, 0.68, 0.50, 1.03 and 0.15, respectively.MNBs and blast aeration showed similar energy consumption for the TN removal.However, MNBs exhibited better potential and economy for NH 3 -N, BOD 5 , COD and TP removal than macro bubbles.

Response of Plankton Composition in Water to MNBs Aeration
The phytoplankton community identified in water samples included Cyanophyta, Chlorophyta, Bacillariophyta, Euglenophyta, Cryptophyta, Pyrrophyta and Cryptophyta, in which the abundance of Cyanophyta, Chlorophyta and Bacillariophyta had absolute superiority.The dominant species of Cyanophyta were Planktothrix and Limnothrix, and the Chlorophyta were dominated by Chlamydomonas.Normally, the disintegration of algae was accompanied by the production of bad taste and odor, and some species even generated a toxic secondary metabolite (e.g., cyanotoxins) that was harmful to animals and humans [1].The zooplankton community identified in the current study included protozoa, rotifers, copepods and cladocerans, and it was dominated by protozoa and rotifers.The protozoa were dominated by Tintinnopsis kiangsuensis, Vorticella sp. and Leprotintinnus fluviatile, with biomasses of 0.75-3.00mg/L, 0.00-2.8mg/L and 0.06-2.76mg/L, respectively.The rotifers were dominated by Brachionus calyciflorus with a biomass of 0.06-1.63mg/L.
Monthly variations in the species and biomass of plankton were observed in Figures 6 and 7. Notably, a surge in zooplankton boom corresponded with a substantial decline in phytoplankton biomass throughout the monitoring process (Figure 6).For instance, in July, zooplankton reached their peak biomass (6.64-16.0mg/L), while phytoplankton biomass was at its lowest (0.45-1.15 mg/L).This trend likely resulted from zooplankton predation on algae.Overall, post-MNBs aeration, plankton biomass was lower compared to other treatments, suggesting MNBs's inhibitory effect on plankton.This reduction can be attributed to two factors: firstly, aeration-induced depletion of nitrogen and phosphorus in water, limiting plankton growth; and secondly, potential disruption of plankton cell structures by MNBs, leading to their rapid settling [41].On the contrary, a low S-W diversity index was recorded (Figure 7), ranging from 0.24 to 1.25 for phytoplankton and 0.00 to 0.70 for zooplankton.This indicates a relatively simple community structure of plankton, with minimal inter-species competition contributing to increased community instability [42].

Impacts of MNBs on Microbial Community in Sediment
Figure 8 illustrates the relative abundance of microorganisms at the phylum various sediment samples.The dominant microbial phylum in original sediment i Chloroflexi, Proteobacteria, Acidobacteriota, Actinobacteria, Firmicutes, Desulfobacter Bacteroidota, accounting for about 80% of the total sequences (Figure 8a).Notabl oflexi and Proteobacteria were the most abundant, which was in accordance with oth ies [1,[43][44][45].Chloroflexi, a photoautotrophic bacterium, typically thrives in nutr adequate environments [43], implying a high nutrient level in the sediment.Bot oflexi and Proteobacteria are known for their ability to decompose organic com [46,47].Additionally, certain species of Proteobacteria are functional microbial gr volved in nitrogen and sulfur metabolism [48].After restoration (5 months), the b community structures in sediments from the control and blast aeration sites show ilarity (Figure 8b,c), suggesting a minimal impact of blast aeration on the microb munity.Conversely, the sediment subjected to MNBs aeration exhibited a signifi

Impacts of MNBs on Microbial Community in Sediment
Figure 8 illustrates the relative abundance of microorganisms at the phylu various sediment samples.The dominant microbial phylum in original sedimen Chloroflexi, Proteobacteria, Acidobacteriota, Actinobacteria, Firmicutes, Desulfobac Bacteroidota, accounting for about 80% of the total sequences (Figure 8a).Nota oflexi and Proteobacteria were the most abundant, which was in accordance with o ies [1,[43][44][45].Chloroflexi, a photoautotrophic bacterium, typically thrives in nu adequate environments [43], implying a high nutrient level in the sediment.B oflexi and Proteobacteria are known for their ability to decompose organic co [46,47].Additionally, certain species of Proteobacteria are functional microbial volved in nitrogen and sulfur metabolism [48].After restoration (5 months), th community structures in sediments from the control and blast aeration sites sh ilarity (Figure 8b,c), suggesting a minimal impact of blast aeration on the micr munity.Conversely, the sediment subjected to MNBs aeration exhibited a sig

Impacts of MNBs on Microbial Community in Sediment
Figure 8 illustrates the relative abundance of microorganisms at the phylum level in various sediment samples.The dominant microbial phylum in original sediment included Chloroflexi, Proteobacteria, Acidobacteriota, Actinobacteria, Firmicutes, Desulfobacterota and Bacteroidota, accounting for about 80% of the total sequences (Figure 8a).Notably, Chloroflexi and Proteobacteria were the most abundant, which was in accordance with other studies [1,[43][44][45].Chloroflexi, a photoautotrophic bacterium, typically thrives in nutritionally adequate environments [43], implying a high nutrient level in the sediment.Both Chloroflexi and Proteobacteria are known for their ability to decompose organic compounds [46,47].Additionally, certain species of Proteobacteria are functional microbial groups involved in nitrogen and sulfur metabolism [48].After restoration (5 months), the bacterial community structures in sediments from the control and blast aeration sites showed similarity (Figure 8b,c), suggesting a minimal impact of blast aeration on the microbial community.Conversely, the sediment subjected to MNBs aeration exhibited a significant alteration in bacterial community structure (Figure 8d).The relative abundance of Actinobacteria, Firmicutes and Myxococcota remarkably increased, and accordingly, Chloroflexi and Proteobacteria were significantly reduced (Fisher's exact test, p < 0.001).MNBs aeration demonstrated a stronger selectivity for microorganisms, effectively inhibiting the growth of anaerobic species in water and promoting aerobic species as the dominant strains [17].These findings corroborate the marked increase in aerobic microorganisms post-MNBs aeration, enhancing the self-purification capacity of the water.
teration in bacterial community structure (Figure 8d).The relative abundance of Actinobacteria, Firmicutes and Myxococcota remarkably increased, and accordingly, Chloroflexi and Proteobacteria were significantly reduced (Fisher's exact test, p < 0.001).MNBs aeration demonstrated a stronger selectivity for microorganisms, effectively inhibiting the growth of anaerobic species in water and promoting aerobic species as the dominant strains [17].These findings corroborate the marked increase in aerobic microorganisms post-MNBs aeration, enhancing the self-purification capacity of the water.At the class level, Gammaproteobacteriam, Anaerolineae, Clostridia, Actinobacteria, Bacilli and Alphaproteobacteria were identified as the dominant bacteria in sediment from both control and blast aeration, whereas sediments treated with MNBs predominantly harbored Gammaproteobacteriam, Anaerolineae, Clostridia, Actinobacteria, Myxococcia and Bacteroidia (Figure 9a).Fisher's exact test revealed significant differences in a total of 15 microorganisms between the control and MNBs aeration treatments (p < 0.006, Figure 9b).Post-MNBs aeration, the relative abundance of Clostridia, Actinobacteria and Myxococcia increased by over 5%, while Acidimicrobiia, Desulfitobacteriia and Ktedonobacteria showed a rise of more than 2%.Particularly, Acidimicrobiia, an oligotrophic bacteria, includes chemoautotrophs capable of oxidizing Fe (II) to Fe (III) using CO2 as a carbon At the class level, Gammaproteobacteriam, Anaerolineae, Clostridia, Actinobacteria, Bacilli and Alphaproteobacteria were identified as the dominant bacteria in sediment from both control and blast aeration, whereas sediments treated with MNBs predominantly harbored Gammaproteobacteriam, Anaerolineae, Clostridia, Actinobacteria, Myxococcia and Bacteroidia (Figure 9a).Fisher's exact test revealed significant differences in a total of 15 microorganisms between the control and MNBs aeration treatments (p < 0.006, Figure 9b).Post-MNBs aeration, the relative abundance of Clostridia, Actinobacteria and Myxococcia increased by over 5%, while Acidimicrobiia, Desulfitobacteriia and Ktedonobacteria showed a rise of more than 2%.Particularly, Acidimicrobiia, an oligotrophic bacteria, includes chemoautotrophs capable of oxidizing Fe (II) to Fe (III) using CO 2 as a carbon source [49,50].Desulfobacterota mediates sulfide oxygenation, and the rate is 1000 to 10,000 times faster than the abiotic oxidation rate [48].Concurrently, the relative abundance of Anaerolineae, Gammaproteobacteria, Bacilli and Alphaproteobacteria decreased by over 2%, with Anaerolineae experiencing a maximum reduction of approximately 10%.These results interpret that MNBs aeration can effectively inhibit the growth of anaerobic microorganisms and reduce their abundance in sediment [51].source [49,50].Desulfobacterota mediates sulfide oxygenation, and the rate is 1000 to 10,000 times faster than the abiotic oxidation rate [48].Concurrently, the relative abundance of Anaerolineae, Gammaproteobacteria, Bacilli and Alphaproteobacteria decreased by over 2%, with Anaerolineae experiencing a maximum reduction of approximately 10%.These results interpret that MNBs aeration can effectively inhibit the growth of anaerobic microorganisms and reduce their abundance in sediment [51].

Conclusions
Compared to conventional aeration, MNBs enhance oxygen transfer and facilitates the removal of organics, nutrients, VOSCs, blacking substances and plankton, thereby rapidly improving water quality.Simultaneously, MNBs promote the transformation of soluble phosphorus into Fe/Al-P in sediment, inhibiting the release of endogenous phosphorus.Furthermore, MNBs augment the activities of aerobic microorganisms, establishing

Conclusions
Compared to conventional aeration, MNBs enhance oxygen transfer and facilitates the removal of organics, nutrients, VOSCs, blacking substances and plankton, thereby rapidly improving water quality.Simultaneously, MNBs promote the transformation of soluble phosphorus into Fe/Al-P in sediment, inhibiting the release of endogenous phosphorus.

Sustainability 2023 , 15 Figure 1 .
Figure 1.Distribution of the experimental sites in the Tangmenbang River.

Figure 1 .
Figure 1.Distribution of the experimental sites in the Tangmenbang River.

Figure 1 .
Figure 1.Distribution of the experimental sites in the Tangmenbang River.

Figure 3 .
Figure 3. Temporal variation in DO, NH3-N, OPR and transparency in water under different aera tions (the dash lines correspond to the light black-odor degree according to the work guide of urba black and odorous water body remediation from China; the letters "O" and "A" represent the orig inal state and the aeration state, respectively).

Figure 3 .
Figure 3. Temporal variation in DO, NH 3 -N, OPR and transparency in water under different aerations (the dash lines correspond to the light black-odor degree according to the work guide of urban black and odorous water body remediation from China; the letters "O" and "A" represent the original state and the aeration state, respectively).

Figure 4 .
Figure 4. Temporal variation in COD, BOD5, TN and TP in water under different aerations.

Figure 4 .
Figure 4. Temporal variation in COD, BOD 5 , TN and TP in water under different aerations.

Figure 5 .
Figure 5. Temporal variation in TOC, NH3-N and Fe/Al-P in sediment under different aerations.

Figure 5 .
Figure 5. Temporal variation in TOC, NH 3 -N and Fe/Al-P in sediment under different aerations.

Sustainability 2023 ,Figure 6 .
Figure 6.Temporal variation in phytoplankton biomass and zooplankton biomass in differ tions.

Figure 7 .
Figure 7. Shannon-Wiener index for phytoplankton and zooplankton in different aeration

Figure 7 .
Figure 7. Shannon-Wiener index for phytoplankton and zooplankton in different aerati

Figure 7 .
Figure 7. Shannon-Wiener index for phytoplankton and zooplankton in different aerations.

Figure 8 .
Figure 8. Composition of microbial community at the phylum level for the sediment from original state (a), control (b), blast aeration (c) and MNBs aeration (d) (the relative abundance of less than 0.01% are uniformly indicated by others).

Figure 8 .
Figure 8. Composition of microbial community at the phylum level for the sediment from original state (a), control (b), blast aeration (c) and MNBs aeration (d) (the relative abundance of less than 0.01% are uniformly indicated by others).

Figure 9 .
Figure 9. (a) Relative abundances of microbial community structures in sediment from different treatments at the class level.(b) The difference in microbial composition and relative abundance of sediment from treatment of control and MNBs aeration at class level (* represented significance: **, p < 0.01; ***, p < 0.001).

Figure 9 .
Figure 9. (a) Relative abundances of microbial community structures in sediment from different treatments at the class level.(b) The difference in microbial composition and relative abundance of sediment from treatment of control and MNBs aeration at class level (* represented significance: **, p < 0.01; ***, p < 0.001).

Table 1 .
Physicochemical properties of water and sediment in Tangmenbang River.