Examining Energy Consumption and Carbon Emissions of Microbial Induced Carbonate Precipitation Using the Life Cycle Assessment Method

: Microbial induced carbonate precipitation (MICP) is a new geotechnical engineering technology used to strengthen soils and other materials. Although it is considered to be environmentally friendly, there is a lack of quantitative data and objective evaluation to support conclusions about its environmental impact. In this paper, the energy consumption and carbon emissions of MICP technology are quantitatively analyzed by using the life cycle assessment (LCA) method. The environmental effects of MICP technology are evaluated from the perspectives of resource consumption and environmental impact. The results show that for each tonne of calcium carbonate produced by MICP technology, 1.8 t standard coal is consumed and 3.4 t CO 2 is produced, among which 80.4% of the carbon emissions and 96% of the energy consumption come from raw materials. Comparing using MICP with cement, lime, and sintered brick, the current MICP application process consumes less non-renewable resources but has a greater environmental impact. The major environmental impact that MICP has is the production of smoke and ash, with secondary impacts being global warming, photochemical ozone creation, acidiﬁcation, and eutrophication. In ﬁve potential application scenarios of MICP, including concrete, sintered brick, lime mortar, mine cemented backﬁll, and foundation reinforcement, the carbon emissions of MICP are 3 to 7 times greater than the emissions of traditional technologies. The energy consumption is 15 to 23 times. Based on the energy consumption and carbon emissions characteristics of MICP technology at the current condition, suggestions are given for the future research of MICP. seawater. This research show that energy consumption and carbon emissions can be signiﬁcantly reduced by using animal waste, eggshell, and seawater in place of industrial urea and calcium sources. Therefore, using organic calcium sources and urea provides a feasible solution to the problems of high-energy consumption


Introduction
Microbial induced carbonate precipitation (MICP) is a bio-mineralization process that refers to microorganisms in rock masses or soils generating calcium carbonate mineral crystals. The MICP process is naturally occurring in many circumstances and can be initiated artificially, under specific environmental and nutritional conditions, to take advantage of the good cementing properties created during the process [1,2]. MICP technology has been successfully applied in many areas of engineering, including foundation reinforcement [3,4], cultural relic restoration [5], anti-seepage and anti-leakage controls [6], heavy metal solidification [7][8][9], mechanical soil improvement [10][11][12], underground mine waste and backfill optimization [13], in addition to a broad range of prospective applications.
There are four types of MICP technology, including urea hydrolysis, denitrification, iron salt reduction reaction, and sulfate reduction reaction. MICP using urea hydrolysis is the most widely used of the 4 processes, having a reaction process that is relatively simple and easy to control and also one that produces a significant amount of carbonates in a short period. Therefore, MICP using urea hydrolysis has been the mainstream technology for calcium carbonate biomineralization [14]. The principal characteristic of MICP urea hydrolysis is that urea is hydrolyzed to carbonate ions by the catalysis of microbial urease. Then carbonate ions react with free calcium ions in the system to produce calcium carbonate precipitation with gelation properties. The mechanism of MICP can be summarized as Formulas (1)-(3) [4,15], and the reaction mechanism model of MICP with urea hydrolyzed bacteria is shown in Figure 1 [16]. Since MICP technology was first introduced, one of its biggest supporting characteristics has been its classification as "environmentally friendly". When comparing MICP to traditional cementation methods, DeJong et al. [17] concluded that MICP provided an alternative and environmentally friendly approach for soil improvement. Nader Hataf [18] used MICP technology to form barriers in waste, water, and foundations, providing an environmentally friendly method for reducing soil permeability of landfill foundations and walls. Li Meng used MICP technology to consolidate heavy metal ions in wastewater to reduce environmental pollution [19]. Gai [20] and Li [21] writes about MICP being used to improve sand and soils and refer to MICP as an environmentally friendly technology in their paper. Adharsh Rajasekar [22] points out that MICP is not 100% environmentally friendly, and the by-products of the reactions can be harmful to human health and local microbiota. To evaluate the environmental effects of MICP scientifically and objectively, energy consumption and carbon emission should be used as indicators for the quantitative analysis.
The consensus viewpoint of current MICP research is that the technology is environmentfriendly, but this viewpoint is often reached without providing evaluations that are thoroughly supported by specific, objective, and systematic methods. This contrasts with other industries where research completed on energy consumption and carbon emissions is often supported by evaluation methods, such as life cycle assessment (LCA), which is a quantitative study of energy consumption and carbon emissions [23]. LCA has been applied to various industries, including cement [24], concrete [25], and steel manufacturing, and the strong results achieved support its use for evaluating MICP. Implementing MICP treatment can be applied through different methods, including the grouting method [19,26], the soaking method [27,28], and the mixing method [13], all, of which have relatively low energy consumption. In addition to having lower energy consumption, MICP technology does not directly produce harmful environmental gases, and beyond this, the technology can absorb carbon dioxide from the air by forming carbonate ions to participate in the reaction. However, though there are not direct environmental impacts of applying MICP technology, creating the raw materials used in MICP-including urease bacteria, urea, and calcium-entails significant energy consumption and carbon emissions.
In this paper, LCA is used to study the energy consumption and carbon emissions of MICP technology. From the perspective of resource consumption and environmental impact, the energy consumption and carbon emissions of MICP technology in different application scenarios are quantitatively analyzed, and the environmental impacts of MICP technology are evaluated. The research conclusion of this paper is not to deny the technical advantages of MICP but to point out the future research direction of MCIP based on its energy consumption and carbon emission performance under the current technical level.

Materials and Methods
LCA is an assessment method that performs "cradle" to "grave" evaluations, which can be used to thoroughly assess the environmental impacts of technologies [24,29]. LCA includes analyzing the extraction of raw materials, the quantification of energy utilization, the transportation of materials, the production process, and long-term and disposal impacts. As defined in International Organization for Standardization (ISO) 14040:2006 [23], LCA is a compilation and assessment of inputs, outputs, and the potential environmental impacts throughout the life cycle of a product. The LCA method is a systematic multi-stage approach and a reliable assessment tool that is divided into four parts: (a) goal and scope definition; (b) inventory analysis; (c) impact assessment of the process; and (d) analysis of the results.

Energy Consumption and Carbon Emission Analysis of MICP Technology Based on LCA
(1) Goal and scope The LCA method is used in this research to study the energy consumption and carbon emissions of MICP technology and evaluate the environmental effects in different applied scenarios using various strength levels.
(2) System boundaries The core functional product of MICP technology is calcium carbonate (CaCO 3 ). When evaluating the life cycle of MICP, CaCO 3 produced by MICP is used as the evaluation index, and the functional unit is set to 1 tonne CaCO 3 . The system boundaries of MICP technology in the analysis process are shown in Figure 2.
(3) Analysis and assessment Based on the system boundaries of MICP technology, a quantitative inventory analysis is conducted for raw material consumption, carbon emissions, and energy consumption within the system. On this basis, by calculating environmental potential (EP) and abiotic depletion potential (ADP), the impact of MICP technology on the environment in different applied scenarios is evaluated.  Natural resources are limited, and as an index of LCA evaluation, abiotic depletion potential (ADP) is a value assigned to a product that is influenced by the amount and type of mineral resources the product consumes in its production. ADP is used to transform the different combinations of consumed resources into a unified reference value that can be used for comparative analyses. The calculation method is shown in Formula (4): where ADP is the total non-renewable resource potential of the constituent elements used to produce the product; MC(i) is the generalized material consumption value of element i; EF(i) is the equivalent factor of element i. The equivalent factor of ADP is shown in Table 1. In the analysis of the MICP system and carbon emissions, other pollutant emissions affect the environment. Therefore, the calculated environmental impact potential value refers to the comprehensive index of total emission impacts of various pollutants as seen in all environments of the entire product system, which can be calculated by Formula (5): where EP(j) is the generalized environmental impact potential value of environment j within the production system; EP(j) i is the environmental impact potential value of the pollutant emission i on the environment j; Q(j) i is the discharge amount of element i on the environment j; EF(j) i is the equivalent factor of the environmental impact potential value of element i on the environment j.
The determination of equivalent factors varies with different environmental impacts, and it usually takes one element as a reference to calculate the relative size of other elements.
According to the methods proposed by the International Standardization Organization (ISO), the International Society for Environmental Toxicology (SETAC), and the Technical University of Denmark, the types of environmental impacts considered in this study are global warming (GW) [30], acidification (AC), eutrophication (NE) [31], photochemical ozone creation (POC) [32,33], and smoke and ash (SA). The main environmental impact types and their corresponding equivalent factors are shown in Table 2. The calculated environmental impact potential values are compared after standardization and weighted assessment.

List of Raw Material Consumption
According to Formulas (1)-(3), 1 mol of CO(NH 2 ) 2 and 1 mol of Ca 2+ can produce 1 mol of cell CaCO 3 under the condition of complete reaction. Thus 0.64 tonnes of urea and 0.4 tonnes of Ca 2+ are needed to produce 1 tonne of calcium carbonate. The ratio of bacterial solution to nutrient salts (urea and CaCl 2 ) is assumed to 1:10. The raw materials used in the whole reaction process of MICP are bacteria solution, urea, and calcium chloride.
(1) Bacterial solution Bacteria and culture medium are needed to prepare the bacterial solution. The bacteria used in this study is Sporosarcina pasteurii, which has a highly effective urease activity and is one of the most popular bacteria in MICP studies [20]. Each liter of the culture medium contains 20 g of yeast extract, 10 g of (NH 4 ) 2 SO 4, and 10 µmol of NiCl 2 . The pH of the culture medium is adjusted to a value of about 8.5-9 by using sodium hydroxide solution.
(2) Urea The theoretical preparation process of urea is shown in Formula (6): high temperature and high pressure The mass ratio of CO(NH 2 ) 2 , NH 3 , and CO 2 is 64:40:44 under complete reaction conditions, which means 0.4 tonnes of NH 3 and 0.44 tonnes of CO 2 are needed to produce 0.64 tonnes of CO(NH 2 ) 2 .
(3) Calcium chloride The theoretical preparation process of CaCl 2 is shown in Formula (7): The mass ratio of CaCl 2 , CaCO 3 , and HCl is 1.08:1:0.72 under complete reaction conditions, which means it takes 1 tonne of CaCO 3 and 0.72 tonnes of HCl to produce 1.08 tonne of CaCl 2 .
The list of raw material consumption to produce 1 tonne of cell CaCO 3 is shown in Table 3.  Table 3 is the optimal ratio during the experiment.

Carbon Emissions of the Bacterial Culture Process
Carbon emissions of the bacterial culture process (C b ) mainly come from the respiration during the bacterial growth and the electrical consumption of equipment used for bacterial cultivation. However, the carbon emission produced by respiration is very small; it is negligible in the calculation. Taking a 1 tonne fermenter as an example, the electrical energy consumed by various instruments during the bacterial cultivation is shown in Table 4. The carbon emission of the bacterial culture process can be calculated by Formula (8): where W i is the power of the instrument i, kW; t is the time, h; k 1 is the carbon emissions generated by 1 MJ electricity, 317 g. According to Formula (8), C b of 1000 L bacterial solution is 667,955.77 g.

Carbon Emissions of Urea and CaCl 2
The energy consumption of urea and calcium chloride (C mu and C mca ) mainly includes coal consumption and electricity consumption, resulting from the consumption of raw materials and the production process. The raw materials and energy consumption in the production process of urea and calcium chloride are shown in Table 5 [34]. According to Table 5, Formulas (4) and (5), each tonne of urea and calcium chloride produce 0.42 tonnes and 2.79 tonnes of CO 2 , respectively. Under complete reaction conditions, MICP technology needs 0.64 tonnes of urea and 1.08 tonnes of calcium chloride to produce 1 tonne of CaCO 3 , and the carbon emission is 2.74 tonnes.

Carbon Emissions from the Reaction Process of MICP
The entire reaction process of MICP occurs naturally and is mainly dominated by bacteria. In the reaction process, no extra energy is required, and only raw materials are constantly consumed. According to Formulas (1) and (2), all CO 2 generated by urea hydrolysis changes to CO 3 2− under the complete reaction condition. This means the carbon emission from the reaction process of MICP (C p ) is 0.

Total Carbon Emissions
Total carbon emissions of MICP (C) include the CO 2 of the bacterial culture process (C b ), the production of raw materials (C mu and C mca ), and the reaction process of MICP(C p ): According to Formula (9), the total amount of carbon emissions of MICP to generate 1 tonne of CaCO 3 is 3399.5 kg.

Comprehensive Energy Consumption
The comprehensive energy consumption (E) of each tonne of calcium carbonate produced by MICP is obtained by converting the coal consumption and electricity consumption into standard coal, as shown in Formula (10): where E is the comprehensive energy consumption of each tonne of calcium carbonate produced by MICP technology, kg coal equivalent (kgce); E C is the coal consumed by MICP technology, kg; E E is the electricity consumed by MICP technology, kWh; k 2 is the standard coal coefficient of raw coal, 0.7143; k 3 is the standard coal coefficient of electricity, 0.1229 kg/kWh. According to the calculation, 1847.3 kgce of energy is consumed to produce 1 tonne of CaCO 3 with MICP technology.   Figure 3 shows that the raw materials of MICP play an important role in carbon emissions and energy consumption. The carbon emissions of MICP mainly come from raw materials, accounting for 80.4% of the total emissions. The rest of the carbon emissions are from the bacterial culture process, accounting for 19.6%. In terms of energy consumption, the coal consumption (E cu and E cca ) of MICP accounts for 80.2% of the total energy consumption, and the electricity consumption (E eu , E eca , E ep and E eb ) accounts for 19.8%. The coal consumption is all from raw materials, and the electricity consumption is 79.8% from raw materials. The energy consumption of raw materials (E cu , E eu , E cca and E eca ) accounted for 96.0% of the total energy consumption. Furthermore, the raw materials are high carbon emission and high-energy consumption materials. The carbon emissions of calcium sources account for 72.4% of the carbon emissions of the entire MICP process. The energy consumption of calcium source and urea account for 53.2% and 42.8% of the total energy consumption, respectively. The energy consumption of the bacterial culture process only accounts for 4.0% of the total energy consumption.

Results and Discussion
To reduce the energy consumption and carbon emissions of MICP technology, many scholars tried other materials to replace calcium chloride and urea. Chen [35] used the pig urine mainly containing ammonia and urea instead of pure urea. The permeability, porosity, and other mechanical properties of the quartz-sand column were improved obviously by MICP with the pig urine. The energy consumption and carbon emissions were reduced by 43% and 8%, respectively, because of using pig urine. Choi [36] made the calcium source by mixing the eggshell and vinegar at a mass ratio of 1:8. The calcium carbonate content of the eggshell is 94%, and the MICP application effect was good. Cheng [37] used seawater as a calcium source of MICP, and the strength of the sand column increased significantly after repeated treatments with seawater. This research show that energy consumption and carbon emissions can be significantly reduced by using animal waste, eggshell, and seawater in place of industrial urea and calcium sources. Therefore, using organic calcium sources and urea provides a feasible solution to the problems of high-energy consumption and high carbon emissions of MICP raw materials. However, the research in this area is far from mature and more research is needed in the future.

The Relationship between MICP Strength Level and CaCO 3 Content
The strength level indicated by the unconfined compressive strength (UCS) of MICP samples directly affects its energy consumption and carbon emissions. The strength level of MICP is related to its calcium carbonate content (CCC). To obtain the quantitative relationship between the UCS and CCC of MICP, the data published by international scholars [10,28,[38][39][40][41][42][43][44][45] are summarized and analyzed. The results are shown in Figure 4 and Table 6.   Figure 4 and Table 6 shows that the strength level of MICP technology increases significantly with the increase of CCC, and there is a power function relationship between UCS and CCC. A representative model to describe the relationship between UCS and CCC of MICP samples can be obtained by regression analysis using all the data from these researchers, as shown in Formula (11).
where S is the unconfined compressive strength, MPa; C is the calcium carbonate content,%; k 4 is the coefficient, 0.00452; k 5 is the index, 2.44197. The quantitative relationship between the strength level and the CCC can provide a basis for the LCA evaluation of energy consumption and carbon emissions of MICP technology in different application scenarios.
Combined with the inventory analysis results, the energy consumption and carbon emissions corresponding to the different strength levels of the MICP are shown in Figure  Figure 5 indicates that the carbon emissions and energy consumption of MICP technology increase rapidly with the increase of the UCS of MICP samples. However, the relationships between strength level and calcium carbonate content of MICP samples are uncertain because of the different MICP materials and methods. The empirical relationship used in this research is an average and representative equation.  Cement is a common binder, which is widely used in the construction industry [46]. However, using cement has the problem of huge energy consumption and environmental pollution [24]. Replacing cement is one of the important potential applications of MICP technology. In addition, many scholars believe that MICP is a more environmentally friendly technology than cement.
The carbon emissions and energy consumption of cement come from the manufacturing process, transportation, fuels, and electricity [47]. The manufacturing process mainly refers to the calcination process of cement. Because CaCO 3 , SiO 2 , Al 2 O 3, and Fe 2 O 3 are decomposed in the clinker under high-temperature conditions [48], a large amount of CO 2 is produced in this process. In the transportation process, it is assumed that all raw materials are transported by rail to the factory with a transportation distance of 100 km. The electricity consumption in cement production includes raw material mining, crushing, pre-homogenization, grinding, homogenization, clinker calcination, coal grinding, cement grinding, transportation, etc.
The fuel is mainly coal in the calcination process of cement production. Coal combustion gives off heat but produces many carbon dioxide emissions. The functional unit in this research is set to 1 tonne of cement, and the carbon emissions and energy consumption of cement production are shown in Table 7 [49].  Table 7 shows that the carbon emissions from cement mainly come from the manufacturing process, accounting for 79.9% of the total carbon emissions. Carbon emissions from transportation, electricity and fuel account for 5%, 14.8%, and 0.3% of the total carbon emissions, respectively. The energy consumed in the cement production process is mainly coal, which accounts for 85.7% of the total energy consumption. All fuel is used in the calcination process and the drying process.
The concrete with a strength of 40 MPa (C40) is chosen to compare the energy consumption and carbon emissions of cement mortar and MICP. The C40 concrete needs about 450 kg of cement per unit volume (1 m 3 ), and its carbon emissions and energy consumption are 468.7 kg and 55.6 kgce, respectively. It can be calculated from Figure 5, to consolidate the same volume of sand and achieve the same strength level by MICP, the carbon emissions and energy consumption is 2107.5 kg and 1145.2 kgce, respectively. The carbon emissions and energy consumption of MICP are 4.5 times and 20.6 times of concrete, respectively. In this application scenario, the carbon emissions and energy consumption of MICP are much higher than cement mortar. MICP is not a more environmentally friendly technology. The main reason is that the raw materials used in MICP, including calcium sources and urea, are all materials with high-energy consumption and high emissions, which harm the LCA environmental evaluation of MICP.
In addition, it is noticed that increasing studies have shown that using MICP technology as an additive in concrete can significantly improve the strength and freeze-thaw resistance of the concrete [50]. In this case, the MICP is used as an admixture rather than a binder, so the environmental impact of MICP should be compared with the traditional admixture in the concrete, not with the cement itself. However, this issue is not discussed due to the limited space of the paper.

Applications of MICP to Replace the Sintered Bricks
Sintered bricks, mainly made of clay, are one of the oldest building materials in the world. It is widely used in the civil and architectural engineering industry with advantages of cheap, durable, fire prevention, heat insulation, noise absorption, and so on [51]. However, the production of sintered bricks needs a huge amount of clay, which destroys cultivated land seriously. According to incomplete statistics, the production of bricks in China damages 467 km 2 of fertile land per year, which is extremely harmful to the environment. MICP technology can be used as a potential alternative to sintering bricks.
For the sintered brick industry, the standard unit brick (SUB) is usually used as the unit to calculate the output of sintered bricks. The volume of a sintered brick is 1,462,800 mm 3 (240 × 115 × 53 mm). The carbon emissions and energy consumption of sintered bricks come from the transportation process, preparation process, fuel combustion, and electricity consumption. The transportation distance is assumed to be 100 km. In the study of this section, the functional unit of sintered brick is set as a SUB. The inventory analysis of the sintered bricks production is shown in Table 8 [52]. The strength of sintered brick is 20 MPa. To produce each sintered brick, 330.69 g CO 2 is emitted, and 0.055 kgce is consumed. When bio-bricks with the same size and strength are manufactured by using MICP technology, the calcium carbonate content is about 31.1%, the carbon emission is 2.3 kg per SUB, and the energy consumption is 1.3 kgce per SUB. The carbon emissions and energy consumption of MICP bio-bricks of the same strength level are 7 times and 23 times higher than those of sintered bricks, respectively. Therefore, the MICP technology has a worse impact on the environment than traditional sintered bricks.
However, the clay used to produce sintered bricks is a non-renewable resource, and the environmental impact of the clay consumption is not considered here. Comparing with sintered bricks, MICP consumes less non-renewable resources, and it is discussed in Section 4.3.

Applications of MICP to Replace the Lime Mortar
Lime, mainly composed of calcium oxide, is obtained by calcining natural rocks containing calcium carbonate at an appropriate temperature to decompose carbon dioxide. As one of the building materials, lime mortar is a mixture of lime, sand, and water in a certain proportion. Lime mortar is widely used in masonry and plastering layers, which requires low strength and is not easy to be damp. The use of lime may damage the water and surrounding vegetation, and lime becomes the second-largest source of greenhouse gas emissions after the cement industry [53]. MICP can be used as a potential substitute technology for lime mortar.
Limestone is the raw material of lime; the reaction principle of the lime production is shown in Formula (12): The production of 1 tonne of calcium oxide needs 2 tonnes of limestone containing approximately 50% of calcium oxide and produces 0.79 tonnes of CO 2 simultaneously. The carbon emissions and energy consumption of lime come from manufacturing, trans-portation, fuel combustion, and electricity consumption. The manufacturing process is the calcining process of limestone, as shown in Equation (12). In the transportation process, it is assumed that all raw materials are transported to the factory by rail, and the transportation distance is 100 km. Fuel combustion and electricity consumption refers to the heat and electricity supply during the process. The functional unit is set to 1 tonne. The carbon emissions and energy consumption of lime produced by different lime kilns are shown in Table 9. Regardless of the lime kiln, the energy consumption and carbon emissions of lime production are very close. The average energy consumption of lime is 149.3 kgce per tonne, and the average carbon emission is about 900 kg per tonne. The carbon emissions produced by lime mainly come from the manufacturing process, accounting for 91.4% of the total emissions. Carbon emissions from transportation, electricity, and fuel accounted for 7.3%, 0.02%, and 1.28% of the total emissions, respectively. The main energy consumed in the lime production process is coal, which accounts for 97% of the total energy consumption, and electricity consumption only accounts for 3%.
The strength of the lime mortar sample is 5 MPa, and the average date is used for calculation. The lime content per unit volume (1 m 3 ) of lime mortar is 216 kg, the energy consumption is 32.3 kgce, and the carbon emission is 193.8 kg. To achieve the same strength level with MICP, the calcium carbonate content is 17.6%, the energy consumption is 488.8 kgce, and the carbon emission is 899.5 kg. The carbon emissions and energy consumption of MICP are 4.6 times and 15.2 times higher than those of lime mortar, respectively. Therefore, in this application scenario, MICP technology has a worse impact on the environment than lime.
However, it should be noted that lime mortar takes a long time to solidify and has poor durability after solidification, which is seriously affected by the moisture [54]. Conversely, the consolidation speed of MICP is faster, and the durability is better [50]. The performance of carbon emissions and energy consumption simply reflects environmental effects, and it does not indicate technical merits.

Applications of MICP to Replace the Cement for Cemented Backfill
The mining process involves the removal and recovery of economically valuable minerals from the crust of the earth [55]. The underground voids caused by mining activities, which may create serious environmental challenges, are expected to be filled with waste materials by a process known as backfilling technology [56]. The underground backfill can support the ground and dispose of the solid waste. It has significant environmental benefits [57]. Backfilling materials mainly include waste rock, gangue, and fly ash, and binder. The binder for backfilling is mainly cement [58]. MICP technology has the effect of cementing instead of cement, so it can be potentially used in cemented backfill mining.
The ratio of backfill materials used in a coal mine is taken as an example. This backfill material comprises 5% cement, 20% fly ash, 55% gangue, and 20% water. The strength of solidified backfill body is 2 MPa. The 1 m 3 of backfill body is taken as a functional unit, the amount of cement is 155 kg, and the carbon emissions and energy consumption are 161.4 kg and 19.1 kgce, respectively.
If the MICP is used to replace the cement in backfill mining, the calcium carbonate content of the MICP backfill body with a strength of 2 MPa is 12.1%. Therefore, the carbon emission of MICP backfill is 618 kg per cubic meter, and the energy consumption is 335.8 kgce per cubic meter. The carbon emissions and energy consumption of MICP backfill materials are 3.8 times and 17.5 times higher than the traditional cement-based backfill materials, respectively. In the application scenario of backfill mining, MICP has no superiority in terms of life cycle environmental benefits comparing with traditional cemented backfill technology.

Applications of MICP to Replace Cement Grouting for Foundation Reinforcement
Foundation refers to the soil layer within a limited area of the building. The unreinforced natural soil layer is called the natural foundation, which is mostly very week. The strength and deformation properties of the natural foundation usually cannot meet the requirements of construction, so the natural foundation mostly needs to be reinforced. Cement grouting is a common reinforcement method. Its principle is to inject the cement slurry into the natural foundation soil to improve its mechanical properties. As one of the main application directions of MICP, MICP grouting reinforcement technology has the advantages of low grouting pressure, good diffusion performance, and low slurry viscosity.
The strength of the foundation after the cement slurry grouting treatment is about 300 kPa-500 kPa. The proportion of cement slurry for grouting reinforcement should be determined according to the actual situation. Generally, 90 kg of cement is required for a unit volume (1 m 3 ) of the cement slurry for foundation reinforcement. The carbon emission per unit volume of cement slurry is 93.7 kg, and the energy consumption is 11.1 kgce. The carbon emission and the energy consumption of MICP grouting with the same strength level are 319.7 kg and 173.7 kgce, respectively. The carbon emissions of MICP grouting are 3.4 times of traditional cement grouting reinforcement, and the energy consumption is 15.6 times of cement grouting. Therefore, MICP shows no obvious advantages from the perspective of carbon emission and energy emission in the application scenario of foundation grouting reinforcement.
However, from a technical point of view, MICP grouting has higher diffusion, lower grouting pressure, and better uniformity of foundation reinforcement than traditional cement grouting. Moreover, MICP grouting is not toxic comparing with chemical grouting. Therefore, although the carbon emissions and energy consumption of MICP grouting are relatively high, its technical advantages are obvious; it has a broad development prospect.

Comparison of Various Application Scenarios
To compare energy consumption and carbon emissions of MICP with traditional technology in different application scenarios, the MICP energy consumption index (k ME ) and MICP carbon emissions index (k MC ) are defined. The k ME is the ratio of the energy consumption of MICP to that of the traditional technology in the current situation. The k ME is the ratio of the carbon emissions of the MICP to that of the traditional technology. When the k ME and k MC are greater than 1, it means that the environmental benefits of MICP are not superior. When the k ME and k MC are equal to 1, it means that the environmental benefits of MICP are equivalent to those of traditional technology. When the k ME and k MC are less than 1, it means that the environmental benefits of MICP are superior in the current situation. The k ME and k MC of MICP in different application scenarios are shown in Figure 6. Under the current technical level, from the perspective of LCA, MICP does not show the advantages of environmental benefits but produces more CO 2 and consumes more energy than traditional technologies. In the high-strength scenarios of 40 MPa and 20 MPa, the k MC index is 4.5 and 7, and the k ME index is 20.6 and 23, respectively. In the low-strength scenarios of 5 MPa, 2 MPa and 0.5 MPa, the k MC index is 4.6, 3.8 and 3.4, and the k ME index is 15.2, 17.5 and 15.6, respectively. In general, k MC and k ME values decrease with the decrease of the strength level of application scenarios. The average k MC and k ME are 4.66 and 18.38, respectively. In other words, the carbon emission of MICP is on average 4.66 times of traditional technologies, and the energy consumption is on average 18.38 times of traditional technologies.
In addition, it should be noted that most data of MICP are obtained on the laboratory scale, while the data of traditional technologies is obtained on an industrial scale. The laboratory scale and industrial scale are very different. With developing MICP technology, its energy consumption and carbon emissions will be greatly reduced. Especially after MICP is applied on the industrial scale, the environmental benefits will be more significant.

Resource Consumption
The five application scenarios involve four kinds of materials: cement, lime, sintered brick, and MICP. The raw materials needed to produce these four materials are shown in Table 10. Abiotic depletion potential (ADP) is used to measure the resource consumption of materials. The functional unit is set to 1 tonne. The ADP is calculated according to Formula (3), and the results are shown in Table 10.  Table 10 shows that the highest ADP value of the four materials is lime, and the MICP has the lowest ADP value. The ADP values of cement, lime, and sintered bricks are all larger than MICP, which shows that more non-renewable resources are needed to produce these materials. This also shows that MICP technology has advantages over cement, lime, and sintered bricks in terms of resource consumption.

Environmental Impact
In the production process of cement, lime, sintered brick, and MICP, besides carbon dioxide, other emissions are produced. The environmental impact potential value is calculated according to Table 2 and Formula (5). The functional unit is set to 1 tonne. The environmental impact potential values of cement, lime, sintered brick, and MICP on different environmental impacts are shown in Figure 7.  Figure 7 shows that cement, lime, sintered bricks, and MICP have the greatest impact on the environment in smoke and dust (SA), followed by global warming (GW) and photochemical ozone creation (POC), and the least impact is acidification (AC) and eutrophication (NE). The greatest impact of MICP on the environment in SA, which is 4 times that of cement, 2 times that of lime, and 48 times that of sintered bricks. The least environmental impact of MICP is NE, which is 3 times of cement, but 50 times of sintered bricks. Under current technical conditions, MICP has the greatest impact on various environmental impacts, while sintered bricks have the least environmental impacts.

Limitation
The energy consumption and carbon emissions studied with LCA can comprehensively and objectively reflect the environmental impact of MICP. However, this research has the following limitations: (1) In this research, the comparison between MICP and traditional technology is carried out in terms of energy consumption and carbon emissions under the current technological level, and it does not represent the merits of the technology itself; (2) The MICP data used in this study are all obtained on the laboratory scale, and the small scale is not good for evaluating the life cycle energy consumption and carbon emissions of MICP. Although the research conclusively shows that MICP is not as environmentally friendly as expected under the current technological conditions, it does not deny this technology. MICP has a great potential for environmental benefits with the development on the industrial scale; (3) Within the life cycle system boundary of MICP technology, the carbon emissions and energy consumption are affected by many factors, such as the respiration of bacteria, the reacting process, the temperature, and the pH. Because the influence of some factors is very small, necessary assumptions and simplifications are made in the research; (4) It is assumed that the urea is completely hydrolyzed and reacted, and the carbon emission from the reaction process is zero. However, the obvious irritating odor of ammonia gas can be smelled during the experiment, which indicates that the urea hydrolysis reaction in the MICP process is not complete. Hence, the actual MICP process needs to consume more urea, which means higher energy consumption; (5) The treatment method for MICP technology includes grouting, soaking, and mixing.
These treatment processes have different technical characteristics, as well as different energy consumption and carbon emissions. However, considering the complexity of the treatment process, it is not covered in the life cycle assessment of MICP in this study.

Prospects
Comparing with traditional cement, lime, and sintered bricks, MICP has technical advantages, such as lower pH, lower viscosity, higher fluidity, lower grouting pressure, better heavy metal ion consolidation performance, and so on. Therefore, although MICP currently does not have an advantage in energy consumption and carbon emissions, it still has great development potential. Based on LCA results of MICP technology at the current technological level, the prospects of MICP research are given as follows: (1) Trying diversified raw materials is an effective way to reduce the carbon emissions and energy consumption of MICP. Some organic materials, such as eggshells and livestock urine, have been studied for MICP, and it is worthy of continuing the related research; (2) Different bacteria besides Bacillus pasteurii can be used for MICP. For example, the carbonic anhydrase mineralizing bacteria can catalyze the hydration reaction of CO 2 and convert it into CO 32 -to achieve the CaCO 3 precipitation. This process can consume CO 2 , thereby significantly reducing the carbon emissions of MICP. In addition, the enzyme-induced CaCO 3 precipitation is a good path to improve environmental benefits; (3) The research of MICP on the industrial scale should be increased. Most of the current research is carried out on the laboratory scale, which is very different from the industrial scale. Usually, the greater scale, the lower unit consumption; (4) The application scenarios of MICP should be more diverse. Besides foundation reinforcement, cultural relic restoration, anti-seepage, and anti-leakage, MICP can be applied in more situations to look for better environmental benefits; (5) The mechanism of MICP needs to be further studied. A clear understanding of the MICP reaction mechanism is very helpful to optimize the MICP process and technical parameters by which carbon emissions and energy consumption can be reduced.

Conclusions
In this paper, the energy consumption and carbon emissions of MICP in different application scenarios are quantitatively analyzed based on LCA. The environmental effects of MICP technology at the current technology level are evaluated. The main conclusions are shown as follows: (1) The energy consumption and carbon emissions of MICP are calculated based on LCA.
Generating 1 tonne CaCO 3 by MICP emits 3399.5 kg of CO 2 and consumes 1847.2 kgce of energy. About 80.4% of carbon emissions and 96% of the energy consumption of MICP are from its raw materials; (2) The relationship between the strength level (UCS) and calcium carbonate content (CCC) of MICP is established. The UCS of MICP increases significantly with the increase of the CCC, and a power function relationship is found between the UCS and CCC of MICP samples. Additionally, due to various influencing factors, this relationship is an average and representative equation; (3) The abiotic depletion potential (ADP) value of MICP is lower than that of cement, lime, sintered bricks, which indicates that MICP consumes less non-renewable resources and has advantages in resource consumption. The greatest environmental impact of MICP is smoke and ash, followed by global warming, photochemical ozone creation, acidification, and eutrophication. Environmental impacts of MICP are more serious than cement, lime, and sintered bricks under current technical conditions; (4) In different application scenarios of concrete, sintered bricks, lime mortar, cemented backfill, and cement grouting foundation reinforcement, the carbon emission of MICP is on average 4.66 times of traditional technologies, and the energy consumption is averagely 18.38 times. It means the environmental benefits of the MICP technology are not superior to those of traditional technology; (5) Although MICP currently does not have an advantage in energy consumption and carbon emissions, it still has great development potential. Suggestions are given for the future research of MICP based on LCA results.