An Online-Based Edu-Escape Room: A Comparison Study of a Multidimensional Domain of PSTs with Flipped Sustainability-STEM Contents

: The use of active and ﬂipped methodologies has increased in recent years. Here, gamiﬁcation uses typical elements of a game in different contexts, including that of education. Speciﬁcally, Escape Room games used as educational tools have potential for teaching–learning, and they can be beneﬁcial because they can improve students’ motivation and emotions toward learning. This is particularly valuable in science, technology, engineering and mathematics (STEM) courses, where the cognitive factor and multidimensional domain are closely connected. This research presents an online-based Edu-Escape Room with science and sustainability contents as an educative tool in a STEM course. With the intervention proposed, we analyze how this tool inﬂuences the multidimensional domain (attitudes, self-efﬁcacy and emotions) of pre-service teachers (PSTs). According to attitude and self-efﬁcacy analysis, it is observed that most of the items analyzed show an increase in self-efﬁcacy and more positive attitudes after the intervention. In particular, Question 11 (Q11) indicates a signiﬁcant difference. Concerning the results for emotion, the positive emotions “joy”, “satisfaction” and “fun” are signiﬁcantly increased after the intervention. However, the negative emotions “nervousness”, “frustration” and “concern” also increase, partly due to the game char-acteristics. The proposed activity had a medium effect on items with signiﬁcant differences except for the emotion “frustration”, where the intervention had a large effect according to effect size (ES) analysis. According to the principal component analysis (PCA), the attitudes, self-efﬁcacy and emotions of the PSTs are positively correlated, and the inﬂuence of the proposed activity shows a signiﬁcant improvement in these variables. Finally, the structural equation modeling partial least squares (SEM-PLS) analysis showed the effects that the instruction has on the PSTs’ emotions and also that they had a signiﬁcant effect on the positive attitudes towards and self-efﬁcacy in science. Therefore, there are multiple beneﬁts in the multidimensional domain of PSTs of having implemented the proposed online-based Edu-Escape Room.


Introduction
With the popular increase in active and flipped learning methodologies in the classroom, the use of games can help to support students' satisfaction, grades, collaboration and motivation for learning [1][2][3]. Thus, game-based education has provided the opportunity for an interdisciplinary approach that takes into consideration how to promote a realistic environment with the aim of achieving a better student experience [2,3]. In this context, Deterding [4] indicated that gamification normally uses the typical elements and techniques of games in different contexts, such as those of business or education. In particular, in university education, various studies show successful practices through the use of gamification [5][6][7]. They even reveal that the students who participate tend to achieve better academic performance, motivation and attitudes towards their courses [5][6][7]. In a experiences and difficulties with science for pre-service teachers (PSTs)allows one to better understand their low self-efficacy and negative attitude towards science teaching, as well as the interrelation between beliefs, self-efficacy and attitude towards science if lasting changes that lead to an improvement in their teaching practices are wanted [36][37][38]. In addition, PSTs' attitudes are important, influencing students' motivation to choose STEM careers and influencing cognitive-affective variables such as self-efficacy, attitudes, motivation and beliefs [37,38]. It is important to generate positive emotions in the teachers in training so that they feel competent and trained to teach STEM, since it depends on them to generate interest in STEM for future generations [39]. Therefore, it is necessary to have a specific study to have a balance the aforementioned as gamification application to improve PSTs' multidimensional domain in a flipped STEM course.
This research aims to analyze the effects of the multidimensional domain of PSTs produced by the online-based Edu-Escape Room used as an educative tool to teach content related to the Universe in a course at the Primary Education degree of the University of Extremadura (Spain). This study focuses on the design, development and subsequent analysis of the online-based Edu-Escape Room. Specifically, we analyzed the intervention affects the attitudes, self-efficacy and emotions of the PSTs. Here, an effect size (ES) analysis was used to measure the magnitude of the activity effect, and it was checked whether they followed any distribution pattern. To complete the study, a principal component analysis (PCA) and the structural equation modeling, partial least squares (SEM-PLS), were conducted with the objective to interpret the variables with significant differences and determine how these variables correlated.

Materials and Methods
In this research, an online-based Edu-Escape Room of contents related to the Universe applied into a flipped class as a teaching tool is analyzed. A comparative study was carried out to analyze attitude, self-efficacy and emotion of PSTs as a multidimensional domain through a questionnaire given before and after the proposed intervention (preand post-test).

Sample and Instruments
The study was conducted with 42 PSTs enrolled in a science course called "Knowledge of the natural environment in primary education", which is taught using a flippedclassroom teaching methodology. The course was taught in the senior course of the Primary Education degree (University of Extremadura, Cáceres, Spain) during the first semester of the 2020/2021 course. No constraints were imposed, and the PSTs freely chose whether they wanted to participate in this research.
The PSTs who participated in the study had a mean age of 23 years old, with 90% in the 15-20 age range and the remaining 10% equally distributed between ages 26-30 and over 40. The PSTs had a mean university entrance grade of 7.46 (scale 1 to 10). It is important to note that the PSTs enrolled in this program had mostly studied Humanities or Social Sciences during their pre-university education (69.05% of the participants). Figure 1 provides more complete demographic information about the sample.
Regarding the instruments used in the study, the collection of data was carried out using a questionnaire with two sections in order to measure the different variables studied: attitude and self-efficacy, and emotions towards science and the proposed intervention. This questionnaire was based on quantitative items that could be measured by a fivepoint Likert scale from 1 to 5. There were a total of 34 items in the questionnaire, i.e., 24 items regarding attitude (Q1 to Q7 correspond to AF_1 to AF_7) and self-efficacy (Q8 to Q24 correspond to ATT_1 to ATT_17) and 10 items (5 positive as E_1 to E_5 and 5 negative emotions as E_6 to E_10) regarding emotions towards science and the intervention proposed (see Table 1).  Regarding the instruments used in the study, the collection of data was carried out using a questionnaire with two sections in order to measure the different variables studied: attitude and self-efficacy, and emotions towards science and the proposed intervention. This questionnaire was based on quantitative items that could be measured by a fivepoint Likert scale from 1 to 5. There were a total of 34 items in the questionnaire, i.e., 24 items regarding attitude (Q1 to Q7 correspond to AF_1 to AF_7) and self-efficacy (Q8 to Q24 correspond to ATT_1 to ATT_17) and 10 items (5 positive as E_1 to E_5 and 5 negative emotions as E_6 to E_10) regarding emotions towards science and the intervention proposed (see Table 1).  First, a 24-item questionnaire was used to measure the attitude and self-efficacy towards science and the proposed intervention, online-based Edu-Escape Room, of the participants. Attitudes towards science and intervention proposed by PSTs were analyzed and measured using the instrument designed by Schruba [40]. Here, the PSTs' attitudes were assessed by considering their favorable or unfavorable feelings towards science and the intervention proposed. Self-efficacy about science and the intervention proposed were measured by various items adapted from the science teaching efficacy belief instrumentpreservice (STEBI-B) [41], developed to analyze the self-efficacy of instructors with regard to science and science teaching. The validity and reliability of both questionnaires (attitudes and self-efficacy) were tested and confirmed in various studies [42][43][44]. For both attitudes and self-efficacy towards science and the intervention proposed, a five-point Likert scale was applied, in which the lowest value was "Strongly Agree" and the highest value was "Strongly Disagreed" before and after intervention implementation (pre-and post-test).
Then, a 10-item questionnaire was used to measure the emotions of the PSTs regarding the expectation of making an online-based Edu-Escape Room about the Universe, along with other science topics, before and after its development (pre-and post-test). This questionnaire has been validated in various research published [39,45,46]. Here, the PSTs were questioned about the intensity of 10 emotions: 5 positive emotions were joy, confidence, satisfaction, enthusiasm and fun, and 5 negative emotions were concern, frustration, uncertainty, nervousness and boredom. They were selected from the emotions that the PSTs could experience in the face of a teaching-learning process according to certain classifications as positive and negative emotion groups of Bisquerra [47]. Specifically, these emotions were quantified from the value of 1 to 5 (a five-point Likert scale), corresponding to the value 1 to never have felt that emotion and the value 5 to have felt it very intensely. The value 3 is the intermediate value, and values 2 and 4 determine "a little" and "sufficient", respectively. Table 1. Questionnaire description about attitude, self-efficacy and emotions. The nomenclature between parentheses refers to the type of item (ATT: attitude, AF: self-efficacy and E: emotion item).

Positive emotion
Negative emotion

Course Context and Procedure
The participants, PSTs, were in the same group, studying the subject in which the intervention was implemented. The course is called "Knowledge of the natural environment in primary education" and is organized into three different blocks of contents. The course contents comprised current challenges in science education and learning to teach science in primary school with different strategies and contents at the primary education stage-such as the importance of science for sustainable development and environmental issues and the role that science has to prevent it-through projects on the curriculum of the knowledge of the natural environment in primary education and resources and didactic materials. This course has a total of 150 h (6 credits). Table 2 provides specific information in the context of the course teaching plan.
Before applying the intervention proposed, the online-based Edu-Escape Room on content related to the Universe, the 42 PSTs answered the questionnaire about attitudes, self-efficacy and emotions towards science and the intervention (pre-test). Due to the COVID-19 outbreak, the activity was made in an online session through Zoom, in which the PSTs connected to a virtual meeting organized by the instructors. In this meeting by videoconference, a synchronous follow-up of the activities completed by the PSTs was developed, clues were provided and doubts were resolved. Therefore, the studentinstructor interaction was closely interchanged during the activity despite not being in a Sustainability 2021, 13, 1032 6 of 18 face-to-face environment. In the context of the online-based intervention proposed, the PSTs completed the Escape Room individually through their computer, tablet or mobile from their respective homes. Finally, as soon as they finished this activity session, they were dedicated to answering the questionnaire that analyzed attitudes, self-efficacy and emotions towards science and intervention proposed after completing the online-based Edu-Escape Room (post-test). All interactive educational materials were provided to the PSTs through the online University platform, Moodle, for a proper teaching and learning process. Table 2. Course description about teaching plan proposed.

General course contents
Study of the natural environment, problem-solving and practical work in the classroom and laboratory, cultural value of science, didactic projection of the relationship between science, technology and society, didactic units in the classroom, resources and didactic materials, interdisciplinarity in the teaching/learning of science, and special educational needs in science teaching/learning.

Topics Contents
T1. Current challenges in science education.
Didactic projection of science, technology and society. Scientific education and transversal topics. Interdisciplinarity in science teaching-learning.
T2. Learning to teach science in primary school through different strategies.
School research, natural environment trips, problem-solving, practical work and project work.
T3. Contents of science education for the primary education stage. Projects on the curriculum of the knowledge of the natural environment in primary education. Resources and didactic materials. To motivate and involve the PSTs to actively participate in this session, the rating of this activity was based on the speed with which the participants solved the onlinebased Edu-Escape Room. Furthermore, the instructors encouraged the interaction and involvement of participants through the online session and had an active role and constant attention to the PSTs' doubts and concerns.

Online-Based Edu-Escape Room Design
The online-based Edu-Escape Room designed deals with the contents related to the Universe; specifically, the theoretical contents of the activity are based on the solar system, the Sun and its role as a source of renewable energy for sustainable development. Other general concepts such as definitions of planets, asteroids, satellites, movements of planets, order in the solar system, density, perimeter, radius and diameter were also studied.
First, the concepts of "gamification" and "Escape Room" used as a teaching tool in the class were explained to the participants. Then, when this theoretical basis was explained and understood, the online-based Edu-Escape Room started as a class activity. It consisted of five assignments that the PSTs should solve during the online session using their computers and other devices individually (see Figure 2). The online-based Edu-Escape Room was designed in such a way that solving a challenge leading to the next task, and it was not possible to advance to the next test without having solved the previous ones.
the class were explained to the participants. Then, when this theoretical basis was explained and understood, the online-based Edu-Escape Room started as a class activity. It consisted of five assignments that the PSTs should solve during the online session using their computers and other devices individually (see Figure 2). The online-based Edu-Escape Room was designed in such a way that solving a challenge leading to the next task, and it was not possible to advance to the next test without having solved the previous ones.  One of the best-known scientists in the study of the Universe is Carl Sagan. As shown in Figure 2, in the context of the activity, he sent a letter to the participants through the university's online platform with all the instructions they must follow to complete the activity (1). This letter guides students to two links; the first to a digital lock (which they could not open because they did not know the password) and the second to a digital puzzle (2) that could be completed by dragging the pieces with the computer mouse. After solving this puzzle, the participants could read the question "What positions do the Sun, Earth and Moon have during the eclipse of the Moon?", and giving a correct solution to this question would unlock the next challenge on the platform. The next task was a crossword puzzle (3) about the general contents of the solar system. Some words in the crossword were marked and, by ordering them in a logical way, the participants already knew that they had to ask for a Quick Response (QR) code from the instructors in order to download the next challenge. The following task (4) consisted of the data about the planets of the solar system (composition, order, satellites and perimeter) that the PSTs should find out by searching in notes, books or on the Internet. Here, some of the words and numbers were marked, and if they were properly arranged, a mathematical operation with a three-digit number is obtained. This numerical combination should be input into the digital lock (5) to reach the final goal and complete the online-based Edu-Escape Room.

Data Analysis
A descriptive analysis was firstly carried out due to its common use as the most appropriate way to characterize, describe and draw conclusions from the data of a sample [48,49]. To measure the reliability and validity of the questionnaires used in the study, Cronbach Alpha Coefficient was applied, demonstrating that the questionnaires used were reliable, as shown in Table 3. To reach plausible conclusions about the effects of the evaluated teaching tool, the homogeneity of the sample was tested. The Shapiro-Wilk normality test was performed to establish whether the data were normally distributed or not. As data were not normally distributed, non-parametric statistical tests were performed. Here, the Mann-Whitney test was used to establish the existence of significant differences between the values of the variables under study before and after the intervention. The ES was also calculated to measure the strength of the relationships between variables. Then, the PCA was calculated to reduce the dimensionality by projecting each data point onto only the first few principal components. In these cases, the statistical software SPSS (SPSS statistics 22.0) was used. Finally, the SEM-PLS analysis was conducted in order to assess the relationship between the variables studied in this research. For this analysis, the SmartPLS software was employed.

Results and Discussion
The data collected through the questionnaire were statistically analyzed to obtain plausible conclusions about the multidimensional domain of PSTs. Once the reliability of the questionnaires using the Cronbach Alpha Coefficient and the non-normality of the data using the Shapiro-Wilk test had been analyzed, the results were classified according to the different statistical tests (comparative analysis of means, ES test, PCA test and SEM-PLS analysis) carried out on each of the variables studied (attitudes, self-efficacy and emotions).

Comparative Analysis of Survey Questionnaires
In order to measure the effects of the online-based Edu-Escape Room proposed on the PSTs multidimensional domain, the answers given by the participants in the 24-item questionnaire about attitude and self-efficacy (7 and 17 items, respectively) were analyzed. Figure 3 shows the comparison of means (scale 1 to 5) of the 24 items about attitude and self-efficacy before and after implementing the online-based Edu-Escape Room (pre-and post-test). It should be noted that most of the items corresponded to issues related to positive attitude and high self-efficacy towards science; a score of 5 on the scale indicated a positive attitude or high self-efficacy. Only items Q15, Q16, Q17, Q18 and Q19 were associated with issues related to a negative attitude and low self-efficacy towards science, so a value of 5 on the scale corresponded to negative attitude or low self-efficacy towards science. Although there was a slight improvement in most items, statistically significant differences were observed only in Question 11 (Q11) "I like the challenges of scientific activities" (p-value = 0.031), where the mean changed from 3.74 to 4.16 before and after the intervention (difference of 0.42 points).  Concerning the emotions felt by the PSTs before and after the intervention, there was an increase in all emotions, except for confidence after the intervention. It was observed that there were significant differences in 6 of 10 emotions analyzed: 3 positive emotions (joy, satisfaction, fun) and 3 negative emotions (nervousness, frustration and concern). Among the positive and negative emotions, enthusiasm and confidence, and uncertainty and boredom, respectively, the differences that were detected were not significant. Figure 4 shows a result on the comparison of means (scale 1 to 5) of these 6 emotions (3 positives and 3 negatives) in which significant differences had been detected before and after implementing the online-based Edu-Escape Room. Particularly, there were differences of 0.43, 0.47, 0.48, 0.94, 1.73 and 0.67 points in happiness, satisfaction, fun, nervousness, frustration and concern, respectively.

Effect-Size (ES) and Principal Component Analysis (PCA) Analysis
An ES analysis measures the strength of the relationship between variables in a statistical population or an estimate of that amount based on a sample. It can refer to the value of a statistic calculated from a sample of data, the value of a parameter in a hypothetical statistical population or the equation that operationalizes how statistics or parameters lead to the value of the ES [50]. Some studies [51,52] indicate that the ES estimation is useful for determining the practical or theoretical importance of an effect, the relative contributions of factors or the power of analysis. It also provides a way to evaluate the data more thoroughly and precisely. In this research, the estimate of the ES was used to measure the magnitude of the treatment effect [53].
Cohen's d is defined as the difference between two means divided by a standard deviation for the data as shown in Equation (1) [53].
S is the pooled standard deviation and is calculated using the following Equation (2) [53]: Depending on the values obtained for the calculation of Cohen's d, the ES of between 0.2-0.3 could be considered "small", around 0.5-0.8 as a "medium" effect, and 0.8-1.0 as a "large" effect [53]. Later, Sawilowsky [54] added to these magnitudes of the effects "very small" when Cohen's d is less than 0.1, "very large" when Cohen's d is around 1.2 and "huge" when Cohen's d is around 2 points.
In this study, we calculated the ES (see Table 4) of the variables that showed signifi-

Effect-Size (ES) and Principal Component Analysis (PCA) Analysis
An ES analysis measures the strength of the relationship between variables in a statistical population or an estimate of that amount based on a sample. It can refer to the value of a statistic calculated from a sample of data, the value of a parameter in a hypothetical statistical population or the equation that operationalizes how statistics or parameters lead to the value of the ES [50]. Some studies [51,52] indicate that the ES estimation is useful for determining the practical or theoretical importance of an effect, the relative contributions of factors or the power of analysis. It also provides a way to evaluate the data more thoroughly and precisely. In this research, the estimate of the ES was used to measure the magnitude of the treatment effect [53].
Cohen's d is defined as the difference between two means divided by a standard deviation for the data as shown in Equation (1) [53].
S is the pooled standard deviation and is calculated using the following Equation (2) [53]: Depending on the values obtained for the calculation of Cohen's d, the ES of between 0.2-0.3 could be considered "small", around 0.5-0.8 as a "medium" effect, and 0.8-1.0 as a "large" effect [53]. Later, Sawilowsky [54] added to these magnitudes of the effects "very small" when Cohen's d is less than 0.1, "very large" when Cohen's d is around 1.2 and "huge" when Cohen's d is around 2 points.
In this study, we calculated the ES (see Table 4) of the variables that showed significant differences between the means before and after the intervention to measure the magnitude of the online-based Edu-Escape Room. These variables consisted of item 11 (Q11) "I like the challenges of scientific activities", the positive emotions "joy", "satisfaction", "fun" and the negative emotions "nervousness", "frustration", "concern". As shown in Table 4, the online-based Edu-Escape Room had a medium effect on all variables with significant differences except for the variable "frustration", where it had a large effect.
The PCA was conducted with the aim to interpret all data collected. The PCA is a method used to describe a data set in terms of new correlated variables (components). The components were ordered by the amount of original variance, so the PCA is useful for reducing the dimensionality of a data set. This tool was useful to summarize large numbers of data and to determine how samples differed from each other (pre-and post-test data), which variables contribute more importantly to this difference and how variables correlated [55,56]. The PCA was done for the variables with statistically significant differences, i.e., Q11 item of attitudes and self-efficacy questionnaire, "I like the challenges of scientific activities" and the emotions "joy", "satisfaction", "fun" (positive emotions), "nervousness", "frustration", "concern". Other emotions analyzed "confidence", "enthusiasm", "uncertainty" and "boredom", which were aggregated as well but did not have much influence. Figures 5 and 6 show a PCA diagrams obtained from this analysis. Figure 5 shows principal component 1 (PC1) and principal component 2 (PC2), which explain 62.9% and 13% of the total variance, respectively. PC1 (62.9% of the variance) can group samples in two groups. One group belongs to positive emotions and is in the positive part of PC1, while negative emotions are assembled in a second group in the negative part of PC1. No sample grouping is observed along PC2. Figure 6 shows principal component 1 (PC1) and principal component 2 (PC2), which explain 34.4% and 30.5% of the total variance, respectively. PC1 (34.4% of the variance) can arrange the sample in two groups (pre-and post-test answers). Pre-test answers are located mainly in the negative part of PC1, while post-test answers are grouped in the positive part of PC1. No sample grouping is observed along PC2. Thus, PC1, which represents the effect of the instruction methodology in the emotions toward activity implemented of PSTs, can distinguish between the PSTs before and after the intervention, presenting more positive emotions after completing the activity. data), which variables contribute more importantly to this difference and how variables correlated [55,56]. The PCA was done for the variables with statistically significant differences, i.e., Q11 item of attitudes and self-efficacy questionnaire, "I like the challenges of scientific activities" and the emotions "joy", "satisfaction", "fun" (positive emotions), "nervousness", "frustration", "concern". Other emotions analyzed "confidence", "enthusiasm", "uncertainty" and "boredom", which were aggregated as well but did not have much influence. Figures 5 and 6 show a PCA diagrams obtained from this analysis.    , which explain 62.9% and 13% of the total variance, respectively. PC1 (62.9% of the variance) can group samples in two groups. One group belongs to positive emotions and is in the positive part of PC1, while negative emotions are assembled in a second group in the negative part of PC1. No sample grouping is observed along PC2. Figure 6 shows principal component 1 (PC1) and principal component 2 (PC2), which explain 34.4% and 30.5% of the total variance, respectively. PC1 (34.4% of the variance) can arrange the sample in two groups (pre-and post-test answers). Pre-test answers are

SEM-PLS Data Analysis
In order to get a better understanding of the influence of the instruction designed on the studied variables, an SEM-PLS analysis was carried out. The model was built grouping the variables into "positive emotions" (E_1 to E_5), negative emotions (E_6 to E_10), "self-efficacy believes" (AF_1 to AF_7) and "attitudes towards science" (ATT_1 to ATT_17). Based on the results, all positive emotions have a positive significant effect of self-efficacy beliefs (t-value: 2.105, p = 0.035), while negative emotions showed a negative significant effect with self-efficacy (t-value: 2.697, p = 0.007). According to the designed model, we also observed that there was a strong significant effect between attitudes and self-efficacy towardss science (t-value: 18.017, p = 0.000). Finally, the loads of each item were close to or greater than the recommended value (>0.700). Considering such values, the reliability, and validity of the constructs can be granted [57]. Results are summarized in Figure 7.

Discussions
Comparison of the results revealed significant differences about the multidimensional domain of the PSTs. Statistically significant differences were observed in the Q11 "I like the challenges of scientific activities" of the attitude and self-efficacy questionnaire, which showed an increase of more than 0.4 points compared to the pre-test. Maintaining positive attitudes and fostering high self-efficacy towards the online-based Edu-Escape Room of science was associated with higher performance and motivation in the contents covered [58,59]. Sequentially, an active teaching methodology such as a virtual Escape Room could lead to increased attitudes and self-efficacy of participants [14,30], which was

Discussions
Comparison of the results revealed significant differences about the multidimensional domain of the PSTs. Statistically significant differences were observed in the Q11 "I like the challenges of scientific activities" of the attitude and self-efficacy questionnaire, which showed an increase of more than 0.4 points compared to the pre-test. Maintaining positive attitudes and fostering high self-efficacy towards the online-based Edu-Escape Room of science was associated with higher performance and motivation in the contents covered [58,59]. Sequentially, an active teaching methodology such as a virtual Escape Room could lead to increased attitudes and self-efficacy of participants [14,30], which was especially useful in STEM courses.
The emotions analyzed showed statistically significant differences in 6 of 10 emotions analyzed: 3 positive emotions-joy, satisfaction and fun-and 3 negative emotionsnervousness, frustration and concern. All these emotions had an increase after the proposed activity. Concerning positive emotions, positive emotional states were encouraged while applying active instructional methodology such as an Escape Room game and flipped classroom [2,27,29,39]. This was especially important in STEM disciplines as students began to show a deficiency of interest in science at an early age, which might impact the adulthood and decreased the choice of STEM careers later in life [30,60]. The increase in negative emotions could be due to a multitude of factors. One of these was the pre-university background of PSTs; most of the sample (69%) had studied Humanities or Social Sciences in High School. This component was associated with lower motivation and the presence of an unfavorable image and negative emotions towards science in general [29,61]. The difficulty of developing the proposed intervention in an online environment might be another factor. Online teaching had various disadvantages such as the feeling of isolation, excessive self-management or the absence of face-to-face interaction between instructors and students. Moreover, it could affect factors such as the student's capacity for independence, the difficulty of follow-up, coordination and communication, and the difficulty of the process in comparison with traditional teaching. This was essential in practical scientific activities that required synchronous follow-up like the proposed activity in this study [59,62].
According to the ES analysis results, it was observed that the online-based Edu-Escape Room has a medium effect on all variables with significant differences except for the variable "frustration". This type of effect was within the acceptable range of ES, and there were some factors such as the heterogeneity of the sample that could decrease the effects of the intervention [63,64]. The online-based Edu-Escape Room had a large effect on the negative emotion "frustration". According to another research study [9,31], when doing an Escape Room, it was important to be aware that it was normal that some negative emotions might appear such as frustration or nervousness due to game components. Frustration might arise for a variety of reasons, such as the difficulty of a challenge, the time taken to solve it or the participant's lack of knowledge. According to some studies, the learning processes that generated knowledge were likely to produce emotions such as frustration, uncertainty, anxiety and boredom [65][66][67]. However, given the complexity of interactions between emotions and variables, emotions such as frustration, anxiety and shame were potential activating elements of the learning process [67].
Regarding the PCA, the variables were arranged into two groups: positive variables (Q11 and positive emotions) and negative variables (negative emotions). Concerning questionnaire answers, there were two groups: pre-test answers and post-test answers. It was observed that pre-test answers were located mainly in the negative part of the x-axis as well as negative variables, and post-test answers were grouped in the positive part of x-axis of the diagram as well as positive variables. Thus, it could students before and after the intervention could be distinguished. There was a positive correlation between the Q11 and positive emotions with the post-test, and there was a correlation between negative emotions and post-test. The results were consistent with other studies [44,56,59]. This information completed the results of the means comparison analysis and the ES analysis. The PCA results confirmed that there was a dependency correlation between the results of the Q11, positive emotions and post-test and between the results of the pre-test and negative emotions. Thus, the proposed online-based Edu-Escape Room could be effective for improving the multidimensional domain of PSTs.
Finally, the SEM-PLS analysis allowed us to determine the effects of the studied variables after applying the online-based Edu-Escape Room developed as an educative tool to teach STEM contents in the University course of the bachelor's degree in Primary Education. According to the results obtained, there PSTs' emotions played a significant effect on the PSTs' self-efficacy beliefs, and these beliefs also have a positive effect on their attitudes towards science.

Conclusions
The study analyses the effects of multidimensional domain of PSTs produced by an online-based Edu-Escape Room developed as an educative tool in the University course within the bachelor's degree in Primary Education at a Teacher Training College (Spain).
According to the results, there is an increase in positive attitudes and high self-efficacy items after the intervention. In particular, Q11, "I like the challenges of scientific activities", had a significant difference and its value increased considerably. Regarding the emotions analyzed, there were significant differences in three positive emotions, "joy", "satisfaction" and "fun" and three negative emotions, "nervousness", "frustration" and "concern". Specifically, there was an increase in these emotions after implementing the online-based Edu-Escape Room. Here, positive emotions were generated due to Escape Room games used as an educative tool, which promoted motivation, interest and fun. Negative emotions were mostly generated because of the difficulties of an online environment. The online-based Edu-Escape Room had a medium effect for items with significant differences except for the emotion "frustration", where the activity had a large effect. Here, negative emotions such as frustration were frequently viewed as detrimental to motivation and learning, but they were also interpreted under some circumstances as beneficial in a learning process. It was also observed that there was a correlation between some items of attitudes, self-efficacy and positive emotions and the post-test, which indicated that there were benefits in the multidimensional domain of the PSTs after having implemented the online-based Edu-Escape Room. Thus, this finding was supported by the SEM-PLS analysis, which showed the tool's effect that the PSTs' emotions influenced to a significant effect of the positive self-efficacy and attitudes towards science. Hence, this tool could be utilized in future works by adding improvements to achieve better results and greater benefits in the multidimensional domain of PSTs. Funding: The authors gratefully acknowledge the Consejerería de Economía, Ciencia y Agenda Digital de la Junta de Extremadura y Fondo Europeo de Desarrollo Regional (FEDER)-Project IB18004, which support this research possible.

Institutional Review Board Statement:
The study was conducted according to the guidelines of the Declaration of Helsinki, and approved by Ethics Committee of University of Extremadura (ref. 94/2018).