The Role of Geometry on a Self-Sustaining Bio-Receptive Concrete Panel for Facade Application

: Bio-receptivity refers to the aptitude of a material to allow for the natural growth of small plant species on stony surfaces with minimum external inﬂuence. Despite the numerous associated environmental beneﬁts, the growth of mosses and lichens on facades has always been viewed as a negative phenomenon due to the random and shabby growth conditions. This research dealt with the design of a self-sustaining bio-receptive concrete facade system with an aim to create a more sustainable and green concrete for the construction industry. The research used surface geometry as a design variable to facilitate moss growth on concrete panels in an ordered and systematic manner. The exercise was an attempt to not only address the functional aspect of bio-receptivity but also its aesthetical quality, which has a primary inﬂuence on people’s perception of bio-receptivity and can promote mass use of this type of concrete material. The research was conducted in a top-down approach, where ﬁrst, through design by research, six distinctly designed concrete panels were fabricated using adapted material composition (blast furnace cement with 75% slag, 0.6 water/cement, sand 0–4 mm and gravel 5–8 mm) as the boundary condition. The concrete mixture together with no curing policy resulted in highly porous concrete panels, suitable for bio-receptive properties. Next in the design validation phase, the inﬂuence of surface geometry/roughness on the water retention ability of the panels and the subsequent moss growth on the panels were evaluated through in vitro experiments. The water retention experiment of the panels was based on quantitative measurements for weight, relative humidity and temperature at several time intervals. The moss-growing experiment was carried out within an ideal greenhouse condition where the panels were initially inoculated with moss spores; the results were based on qualitative observation for a period of 4 months. According to the comparative analysis of these results, with the same material composition, Panel 2 showed the highest bio-colonization owing to its prominent surface geometry, whereas Panel 5 showed the least bio-colonization owing to its plain surface despite high absorption capacity. Thus, the role of geometry has been extensively proven in this research and as an outcome a set of general design guidelines have been formulated for a self-sustaining bio-receptive concrete facade panel, using geometry as a design variable for bio-receptivity.


Introduction
Concrete is one of the most popular construction materials in the world due to its strength and durability. However, despite its benefits, concrete production is also responsible for 8% of the global CO 2 emissions [1]. A literal way to offset this is to grow plants on concrete to facilitate CO 2 uptake [2][3][4][5]. Designing a concrete material that can be host In line with the cited research experiences, this research is a further exploration into the relevance of geometrical articulation of a surface on its potential for bio-receptivity. Here, the problem has been addressed to stimulate bio-receptivity through solutions based on real time practical experimentations. The main objective of this research was to explore the impact of geometry on the ordered growth of mosses, in order to highlight its benefits and promote (mass) the use of these bio-receptive concrete facade panels. Geometry has been used to facilitate the ordered and systematic growth of mosses on concrete facade panels, resulting in an aesthetically pleasing and functional surface layer. The research followed a top-down approach where, first, designs were developed based on the literature and field surveys, and these were then validated through practical experimentations and simulations, finally providing a general design guideline for the use of geometry as a design variable in the development of bio-receptive facade panels.

Strategy and Methods
To achieve the research goals, the methodology followed here was a top-down approach. The approach was divided into two main stages. The first stage involved the formulation of the design scenarios, where designs were developed based on literature research and field surveys. The second stage was the experimental setup, where the designs were validated through experimentation to create a set of design guidelines that can enhance the bio-receptive quality of concrete panels. In the first stage, the boundary conditions were set for the concrete mixture according to the research results from the doctoral dissertation of Manso [3], used to fabricate the designed panels. In the second stage the moss-growing experiment was carried out within a controlled environment in a greenhouse, assuming an ideal growth condition (temperature 20-24 °C, RH 75-85%), while the water retention experiment was setup in a laboratory replicating the effect of rainfall onto the surface of the concrete panels. Due to time restraints, carrying out the experiments in outdoor conditions was not within the scope of this research.

Definition of the Design Scenarios
In this section, the first stage of the top-down approach has been discussed, where following the design of the research strategy, prototype designs were developed. The research began with a thorough literature review on the plant biology of mosses, material properties of concrete, environmental properties, influence of geometry on bio-receptivity and field surveys.
The literature review and practical insight from the field survey provided an outline to design several geometrical options to facilitate engineered moss growth on concrete. According to the studies, mosses are non-vascular plants, with no root system, which take The main objective of this research was to explore the impact of geometry on the ordered growth of mosses, in order to highlight its benefits and promote (mass) the use of these bio-receptive concrete facade panels. Geometry has been used to facilitate the ordered and systematic growth of mosses on concrete facade panels, resulting in an aesthetically pleasing and functional surface layer. The research followed a top-down approach where, first, designs were developed based on the literature and field surveys, and these were then validated through practical experimentations and simulations, finally providing a general design guideline for the use of geometry as a design variable in the development of bio-receptive facade panels.

Strategy and Methods
To achieve the research goals, the methodology followed here was a top-down approach. The approach was divided into two main stages. The first stage involved the formulation of the design scenarios, where designs were developed based on literature research and field surveys. The second stage was the experimental setup, where the designs were validated through experimentation to create a set of design guidelines that can enhance the bio-receptive quality of concrete panels. In the first stage, the boundary conditions were set for the concrete mixture according to the research results from the doctoral dissertation of Manso [3], used to fabricate the designed panels. In the second stage the moss-growing experiment was carried out within a controlled environment in a greenhouse, assuming an ideal growth condition (temperature 20-24 • C, RH 75-85%), while the water retention experiment was setup in a laboratory replicating the effect of rainfall onto the surface of the concrete panels. Due to time restraints, carrying out the experiments in outdoor conditions was not within the scope of this research.

Definition of the Design Scenarios
In this section, the first stage of the top-down approach has been discussed, where following the design of the research strategy, prototype designs were developed. The research began with a thorough literature review on the plant biology of mosses, material properties of concrete, environmental properties, influence of geometry on bio-receptivity and field surveys.
The literature review and practical insight from the field survey provided an outline to design several geometrical options to facilitate engineered moss growth on concrete. According to the studies, mosses are non-vascular plants, with no root system, which take in water through capillary action. Mosses require water to carry out the fertilization process and wind for spore dispersal [8]. Being poikilohydric, mosses that are found in exposed surfaces often grow in tufts or cushion form to minimize water loss [16]. Mosses, being small plant species, need to have a microclimate on and above the surface. The micro-climate can help to maintain the required moisture level, a cooler temperature and a low wind speed. The role of geometry is vital to create the desired micro-climate conditions for this plant growth.
An engineered growth refers to employing surface geometry to create a self-sustaining system for the growth of mosses in desired areas and in a desired coverage. The presence of water is essential for the growth and development of bio-colonization [17]. The surface roughness, being the most crucial physical characteristic of the material, should be able to retain water from the rain, dew and other natural phenomena on its surface [12,17,18]. The surface morphology can also help to create areas of shading to protect plants from direct sunlight and provide buffers to high wind speed causing detachment and also gather nutrients onto its surface.
Based on this parameter, four distinct geometric panels were designed, catering to the environmental factors for a self-sustaining system ( Figure 2). The geometric features of each designed panel were classified into two geometry levels, macro-and micro-level geometry. A combination of both these geometric levels helped to create a micro-climate within its surface by influencing the water flow path of the panels and direct growth in desired areas. The micro-geometric features, called micro-grooves, were used where moss coverage was desired, creating slow water movement into water catchment areas and anchorage facilities. The macro-geometric features created the overall surface undulations, which helped to distinguish between the growth and no-growth areas. Two extra panels, a plain concrete panel and a naturally rough concrete panel with exposed sand and gravel layers, were also created for a more vivid comparative analysis between the ordered and random surface geometries ( Figure 2). in water through capillary action. Mosses require water to carry out the fertilization process and wind for spore dispersal [8]. Being poikilohydric, mosses that are found in exposed surfaces often grow in tufts or cushion form to minimize water loss [16]. Mosses, being small plant species, need to have a microclimate on and above the surface. The micro-climate can help to maintain the required moisture level, a cooler temperature and a low wind speed. The role of geometry is vital to create the desired micro-climate conditions for this plant growth.
An engineered growth refers to employing surface geometry to create a self-sustaining system for the growth of mosses in desired areas and in a desired coverage. The presence of water is essential for the growth and development of bio-colonization [17]. The surface roughness, being the most crucial physical characteristic of the material, should be able to retain water from the rain, dew and other natural phenomena on its surface [12,17,18]. The surface morphology can also help to create areas of shading to protect plants from direct sunlight and provide buffers to high wind speed causing detachment and also gather nutrients onto its surface.
Based on this parameter, four distinct geometric panels were designed, catering to the environmental factors for a self-sustaining system ( Figure 2). The geometric features of each designed panel were classified into two geometry levels, macro-and micro-level geometry. A combination of both these geometric levels helped to create a micro-climate within its surface by influencing the water flow path of the panels and direct growth in desired areas. The micro-geometric features, called micro-grooves, were used where moss coverage was desired, creating slow water movement into water catchment areas and anchorage facilities. The macro-geometric features created the overall surface undulations, which helped to distinguish between the growth and no-growth areas. Two extra panels, a plain concrete panel and a naturally rough concrete panel with exposed sand and gravel layers, were also created for a more vivid comparative analysis between the ordered and random surface geometries ( Figure 2).  To maintain an aesthetically pleasing look to the designed geometric panels, the geometric compositions investigated in this research were inspired by patterns found in nature depicting different functional qualities. The patterns were further translated into surface geometry on the four panels through a method of repetition called rhythm, creating a sense of harmony and order that is aesthetically pleasing to the human eye ( Figure 3) [19]. Panel 1 has been designed in an alternating rhythm method, Panel 2 in a flowing rhythm, Panel 3 in a regular rhythm and Panel 4 followed a random rhythm.
To maintain an aesthetically pleasing look to the designed geometric panels, the geometric compositions investigated in this research were inspired by patterns found in nature depicting different functional qualities. The patterns were further translated into surface geometry on the four panels through a method of repetition called rhythm, creating a sense of harmony and order that is aesthetically pleasing to the human eye ( Figure 3) [19]. Panel 1 has been designed in an alternating rhythm method, Panel 2 in a flowing rhythm, Panel 3 in a regular rhythm and Panel 4 followed a random rhythm. To achieve bio-receptivity, the concrete needs high surface roughness with high porosity and a neutral pH level [2]. To further proceed with a foolproof concrete mixture with bio-receptive properties, the recipe was adapted from the doctoral dissertation of Manso. Factors like the type of binder to be used, the aggregate sizes, the water/cement ratio and the amount of cement paste determined the material composition for bio-receptivity [3]. Table 1 shows the material content for 1 m 3 of concrete. For detailed calculation, refer to Appendix A ( Figure A1).

Materials
Quantity (kg) CBR CEM III/B 32.5 N Blast furnace cement (75% slag content) 300 Dry sand (0-4 mm) 740 Dry Jurassic yellow gravels (5-8 mm) 1142 Water (water cement ratio, w/c 0.6) 180 The prototype making process was carried out as following. The negatives of the four prototype designs were fabricated through CNC milling on hard foam material. The foam molds were next brushed with a sealing agent, silicone, to reduce their adhesion quality to concrete and ensure easy demolding ( Figure 4a). Finally, after 24 h. of hardening, the molds were filled with the prescribed concrete mixture (Figure 4b). The mixture was left to set uncovered in the molds for 48 h without any curing to achieve the highest possible porous quality of the concrete panels. In total, 12 concrete panels in the size 350 × 250 × 40 mm were made. For each tile, 3.25 L of the mixture were cast into the molds. Among the two sets of the concrete panels cast, one set was used for the macro-level water-related laboratory testing and the other set for the controlled moss growth experiment. To achieve bio-receptivity, the concrete needs high surface roughness with high porosity and a neutral pH level [2]. To further proceed with a foolproof concrete mixture with bio-receptive properties, the recipe was adapted from the doctoral dissertation of Manso. Factors like the type of binder to be used, the aggregate sizes, the water/cement ratio and the amount of cement paste determined the material composition for bio-receptivity [3]. Table 1 shows the material content for 1 m 3 of concrete. For detailed calculation, refer to Appendix A ( Figure A1).

Materials Quantity (kg)
CBR CEM III/B 32.5 N Blast furnace cement (75% slag content) 300 Dry sand (0-4 mm) 740 Dry Jurassic yellow gravels (5-8 mm) 1142 Water (water cement ratio, w/c 0.6) 180 The prototype making process was carried out as following. The negatives of the four prototype designs were fabricated through CNC milling on hard foam material. The foam molds were next brushed with a sealing agent, silicone, to reduce their adhesion quality to concrete and ensure easy demolding ( Figure 4a). Finally, after 24 h. of hardening, the molds were filled with the prescribed concrete mixture (Figure 4b). The mixture was left to set uncovered in the molds for 48 h without any curing to achieve the highest possible porous quality of the concrete panels. In total, 12 concrete panels in the size 350 × 250 × 40 mm were made. For each tile, 3.25 L of the mixture were cast into the molds. Among the two sets of the concrete panels cast, one set was used for the macro-level water-related laboratory testing and the other set for the controlled moss growth experiment.

Water Retention Experiment
As already found, water is essential for the growth and survival of mosses. The designed geometric patterns on the concrete panels have been used to influence the water flow movement on and within its surface. To test and validate this hypothesis, the following water retention testing was carried out in a laboratory setup. The goal of this experiment was to measure how much the weight of each of the panels changed due to their water absorption and retention capacities, which were solely influenced by the surface

Water Retention Experiment
As already found, water is essential for the growth and survival of mosses. The designed geometric patterns on the concrete panels have been used to influence the water flow movement on and within its surface. To test and validate this hypothesis, the following water retention testing was carried out in a laboratory setup. The goal of this experiment was to measure how much the weight of each of the panels changed due to their water absorption and retention capacities, which were solely influenced by the surface geometry of each panel irrespective of their material composition.
The experiment began with the laboratory setup as shown in Figure 5, the panel was placed over a waffle grill to allow the sprayed water to drain out into the tray below. Next, a camera was set from a suitable distance to record the water movement on the surface. For better visibility, water soluble beetroot juice was diluted with water for spraying. A pressure water sprayer with a 30 • nozzle angle was filled with the red-color water and positioned at a fixed distance of 25 cm from the concrete block. Black tap markings were used to demark the standing position for spraying, the distance of the spray nozzle from the block and the position of the tripod for the camera. The room temperature was measured to be 22 • C and RH 55% for the record. The ridge part of the designed panel surfaces was sand polished to smoothen it for efficient redirection of water from ridges towards the alcove. Next, the panels were oven dried for a period of 6 h at 45 °C . The oven drying was only done once at the beginning of the experiment to ensure complete drying of the panels. The experiment was carried out in the following steps: Step 1: The weight before spraying was recorded.
Step 2: The panels were calibrated vertically according to the setup described in the previous section.
Step 3: The colored water measuring 150 mL was sprayed for a duration of 30 s, in an up and down motion of the sprayer to allow even wetting of the panel.
Step 4: After a period of 2 min, the amount of water drained out into the tray was measured and the readings for temperature, surface relative humidity and weight of the panels were recorded.
Step 5: The temperature, humidity and weight readings were recorded twice more, after 10 min and 20 min from the spraying time (Figure 6a-c). The ridge part of the designed panel surfaces was sand polished to smoothen it for efficient redirection of water from ridges towards the alcove. Next, the panels were oven dried for a period of 6 h at 45 • C. The oven drying was only done once at the beginning of the experiment to ensure complete drying of the panels. The experiment was carried out in the following steps: Step 1: The weight before spraying was recorded.
Step 2: The panels were calibrated vertically according to the setup described in the previous section.
Step 3: The colored water measuring 150 mL was sprayed for a duration of 30 s, in an up and down motion of the sprayer to allow even wetting of the panel.
Step 4: After a period of 2 min, the amount of water drained out into the tray was measured and the readings for temperature, surface relative humidity and weight of the panels were recorded.
Step 5: The temperature, humidity and weight readings were recorded twice more, after 10 min and 20 min from the spraying time (Figure 6a-c).
Step 3: The colored water measuring 150 mL was sprayed for a duration of 30 s, in an up and down motion of the sprayer to allow even wetting of the panel.
Step 4: After a period of 2 min, the amount of water drained out into the tray was measured and the readings for temperature, surface relative humidity and weight of the panels were recorded.
Step 5: The temperature, humidity and weight readings were recorded twice more, after 10 min and 20 min from the spraying time (Figure 6a-c). The procedure was repeated for day 3 and day 7 to test the water retention ability of the panels over a period of 7 days. The whole laboratory setup and experiment procedure was repeated a second time to check for any discrepancies in the two sets. In the results section, all the data from the experiment have been thoroughly documented and analyzed to further deduce the findings.

Moss-Growing Experiment
The second set of the freshly cast panels were used for the moss-growing experiment.
The experiment was carried out in the greenhouse of the TU Delft botanical garden. The experiment was dated between the end of February to mid-May, after a trial phase for the first three weeks of February.
The saxicolous moss fragments with rip spores were collected from the botanical garden stone surfaces. The slurry was prepared, according to the findings of our literature review. Buttermilk and moss fragments in a (1:1) ratio were blended for 24 h before application. The buttermilk helped to create an acidic base for the mixture. The panels were prepared by polishing the ridges with sandpaper for a smoother finish where no moss growth is desired and then thoroughly cleaned with water and vinegar to remove any surface contaminants. The prepared slurry was painted in desired areas using a paint brush.
Based on the findings of the trial period, the panels recoated with moss slurry were placed in the warmer tropical greenhouse. The experiment was carried out in a span of 12 weeks. It was done in two phases, where during first 6 weeks, the panels were placed in horizontal position, and for the last 6 weeks, in an upright position ( Figure 7). The temperature ranging between 20-24 • C and RH 75-85% was maintained within the greenhouse and rainwater was sprayed onto the panels twice or thrice daily for around 30 s each time depending on the weather conditions. During the dark cycle of the day, the panels were kept uncovered due to the high humidity (RH 60%+) inside the greenhouse. The procedure was repeated for day 3 and day 7 to test the water retention ability of the panels over a period of 7 days. The whole laboratory setup and experiment procedure was repeated a second time to check for any discrepancies in the two sets. In the results section, all the data from the experiment have been thoroughly documented and analyzed to further deduce the findings.

Moss-Growing Experiment
The second set of the freshly cast panels were used for the moss-growing experiment.
The experiment was carried out in the greenhouse of the TU Delft botanical garden. The experiment was dated between the end of February to mid-May, after a trial phase for the first three weeks of February.
The saxicolous moss fragments with rip spores were collected from the botanical garden stone surfaces. The slurry was prepared, according to the findings of our literature review. Buttermilk and moss fragments in a (1:1) ratio were blended for 24 h before application. The buttermilk helped to create an acidic base for the mixture. The panels were prepared by polishing the ridges with sandpaper for a smoother finish where no moss growth is desired and then thoroughly cleaned with water and vinegar to remove any surface contaminants. The prepared slurry was painted in desired areas using a paint brush.
Based on the findings of the trial period, the panels recoated with moss slurry were placed in the warmer tropical greenhouse. The experiment was carried out in a span of 12 weeks. It was done in two phases, where during first 6 weeks, the panels were placed in horizontal position, and for the last 6 weeks, in an upright position ( Figure 7). The temperature ranging between 20-24 °C and RH 75-85% was maintained within the greenhouse and rainwater was sprayed onto the panels twice or thrice daily for around 30 s each time depending on the weather conditions. During the dark cycle of the day, the panels were kept uncovered due to the high humidity (RH 60% +) inside the greenhouse.

Results of Water Retention Experiment
The results for the water retention experiment were analyzed in four sections: change in weight, ridge and alcove relative humidity, change in temperature and visual analysis of water movement on the surface of the six panels.

Results of Water Retention Experiment
The results for the water retention experiment were analyzed in four sections: change in weight, ridge and alcove relative humidity, change in temperature and visual analysis of water movement on the surface of the six panels.

Change in Weight
In Figure 8a, the graph shows an average change in weight value at 2 min and 20 min after spraying for the total 6 days of readings. Due to a gradual drop in weight at 2 min, 10 min and 20 min, the bar chart only shows the change in weight values for 2 min and 20 min. The average change in weight expressed as a percentage of its initial weight at 2 min was found to be highest for panel 6 and panel 5, respectively. Panel 6 showed the highest weight gain owing to its natural surface roughness, while panel 5, contradicting its plain surface, showed the second highest weight gain. Due to the absence of a surface repellent layer, panel 5 exhibited the high porous quality of the concrete itself. Panel 3 also showed The average change in weight expressed as a percentage of its initial weight at 2 min was found to be highest for panel 6 and panel 5, respectively. Panel 6 showed the highest weight gain owing to its natural surface roughness, while panel 5, contradicting its plain surface, showed the second highest weight gain. Due to the absence of a surface repellent layer, panel 5 exhibited the high porous quality of the concrete itself. Panel 3 also showed moderately high weight gain value owing to the water catchment into its deeper recessed spaces, but it also showed the highest weight lost at 20 min due to loss of its surface water. Panels 1, 2 and 4 showed the least weight gain at 2 min, mostly owing to their shallow micro-grooves and the presence of the surface repellent layer adhered from the mold surface while casting. Figure 8b shows a cumulative display of values for weight gain at 2 min for the two sets of experiments. Panel 6 and panel 5 showed the highest gains, with a nearly steady increase in weight gained at 2 min for all six days. Panel 3 showed the highest fluctuation in weight gain due to surface water lost during transport for weight measurement. An over-time increase in weight gain was seen for Panels 6, 5 and 3, which is presumed to be due to a break in water tension in the capillary pores of the concrete panels. However, Panels 1, 2 and 4 showed a mirror result for set 2, owing to their poor water relation qualities. Figure 8c shows a cumulative result for weight lost at 20 min for all the six days of the experiment. Panel 3 showed the highest weight loss with the highest fluctuation among the six days, which was due to different amounts of surface water lost on each day. Panel 6 showed an improvement in absorption capacity, with descending weight loss values in set 2, owing to its natural rough surface. The rest of the panels showed high fluctuation in weight loss in set 1 moving towards lower fluctuations in set 2, with Panel 2 reaching a flattened curve.
Therefore, from the two sets of values, it can be seen, that panel 6 with natural roughness showed the highest weight gain and very low weight loss along the six days. This can be explained due to the absence of the surface repellent layer and the deeper surface roughness. In terms of the designed panels, it was noticed that the panels with deeper recessed spaces and micro-grooves retained more water on the surface, resulting in more water absorption into the panels, and these were found to be two important learnings to be further implemented into the design guidelines.

Alcove and Ridge Relative Humidity
Due to the 3D patterns on the surface, largely two horizontal surface planes were present, made up of an alcove, the recessed part and ridge, and the raised part, giving two types of relative humidity (RH) values. The alcove/ridge readings for the RH were taken at three different points, top, middle and bottom along the middle of the panels (Figure 9a). This was done to get a more distinct range of moisture values through the concrete surface. The alcove/ridge readings were taken at 2 min, 10 min and 20 min after spraying and repeated for two sets of experiment.
As in Figure 9b, for alcove moisture, panel 3 showed the highest RH values at both 2 min and 20 min, with a slight dip in alcove moisture at 10 min. The high value was due to the presence of deeper alcove spaces with 4 mm deep micro-grooves, positioned against the flow obstacles, which created more hindrance to the linear flow path compared to other panels. Panel 6 showed a significant drop in alcove moisture from 2 min to 20 min due to both its high absorption capacity and high surface evaporation. All other panels showed low and nearly constant RH values, due to their shallow groove depths.
As in Figure 9c, Panel 3 showed an increase in ridge moisture over time, owing to its against the flow macro geometry, which gradually got more wet as the water dripped down from the alcove to the ridges. For Panel 6, the ridge moisture was the same as the alcove moisture results. All the other panels showed low and nearly constant values through time, with panel 2 showing the lowest ridge moisture due to its lower ridge surface area. As in Figure 9b, for alcove moisture, panel 3 showed the highest RH values at both 2 min and 20 min, with a slight dip in alcove moisture at 10 min. The high value was due to the presence of deeper alcove spaces with 4 mm deep micro-grooves, positioned against the flow obstacles, which created more hindrance to the linear flow path compared to other panels. Panel 6 showed a significant drop in alcove moisture from 2 min to 20 min due to both its high absorption capacity and high surface evaporation. All other panels showed low and nearly constant RH values, due to their shallow groove depths.
As in Figure 9c, Panel 3 showed an increase in ridge moisture over time, owing to its against the flow macro geometry, which gradually got more wet as the water dripped down from the alcove to the ridges. For Panel 6, the ridge moisture was the same as the alcove moisture results. All the other panels showed low and nearly constant values through time, with panel 2 showing the lowest ridge moisture due to its lower ridge surface area.
Therefore, it could be concluded that greater surface undulations help the water to dry out slowly, working as a shield, as was evident from the results of Panel 6. Though at 2 min, it showed high surface moisture, at 20 min, due to it very low surface undulations, its rate of evaporation was also very high. Another aspect that was noticed was the against the flow macro-geometries were contradicting the idea of distinct growth and no-growth Therefore, it could be concluded that greater surface undulations help the water to dry out slowly, working as a shield, as was evident from the results of Panel 6. Though at 2 min, it showed high surface moisture, at 20 min, due to it very low surface undulations, its rate of evaporation was also very high. Another aspect that was noticed was the against the flow macro-geometries were contradicting the idea of distinct growth and no-growth areas. As it was seen in case of Panel 3, the water dripped down from the alcove to the ridges, blurring the surface distinction desired.

Change in Temperature
The temperature readings were taken to see the cooling effect of the concrete panels after spraying water onto the surfaces. All the panels showed an initial temperature of 20-21 • C. After spraying at 2 min, the panels showed a sharp decrease in temperature by 2-3 • C and in the 10 min and 20 min readings, the temperature gradually increased by 0.5 • C. The results were found to be similar for all the panels, which could be due to the same material composition of the panels. The different surface geometries seemed to have no significant effect on the cooling effect of the surface, and thus the results were not further analyzed.

Visual Representation of Surface Water Movement
As in Figure 10, the visual representation helped to get a live picture of the behavior of water on the surface. These visual results of the water movement pattern on the surfaces helped to further strengthen the results from the change in weight, and ridge and alcove relative humidities. The accumulated results from the water retention experiment were used to draw out a practically driven and verified set of design guidelines to enhance the bio-receptivity characteristics of the concrete panels.

Trial Results
During the 3 week trial, some important information was learned, which greatly fluenced the moss growth. The best temperature to accelerate moss growth was found be between 20-24 °C, though according to literature, the most suitable temperature w around 15 °C . The growing process was prolonged in the colder temperature and was suitable for a controlled growth within a short time. After about 7-8 days of applicati signs of fungus growth were observed over the applied slurry. This process is called sy biosis, where two organisms living together undergo an ecological interaction. Symbio is known to exist in the early stages of plant growth, where both the parties benefit fr

Trial Results
During the 3 week trial, some important information was learned, which greatly influenced the moss growth. The best temperature to accelerate moss growth was found to be between 20-24 • C, though according to literature, the most suitable temperature was around 15 • C. The growing process was prolonged in the colder temperature and was not suitable for a controlled growth within a short time. After about 7-8 days of application, signs of fungus growth were observed over the applied slurry. This process is called symbiosis, where two organisms living together undergo an ecological interaction.
Symbiosis is known to exist in the early stages of plant growth, where both the parties benefit from an increase in the uptake of nutrients [20]. This stage is the early colonizing phase by the fungi and spores before the appearance of moss ( Figure 11). an increase in the uptake of nutrients [20]. This stage is the early colonizing phase by the fungi and spores before the appearance of moss ( Figure 11). Figure 11. Fungus growth on the panels (symbiosis).

Bio-colonization Results
After the trial period, the first 6 weeks, the panels were kept in a horizontal position to allow them to remain wet for a longer time and create the required moist conditions for moss growth. Around the fourth week, small patches of green algae were seen on the surface of Panel 2, while the others remained unchanged as dark brown. During the sixweek, bright green layers of algae had grown in the central alcoves of Panel 2, while light patches were also seen in Panels 1, 3 and 4, mostly around their central areas, due to the higher concentration of water spraying along this region. Among all the panels, the macro geometry of Panel 2 had the greatest depth, which allowed higher water retention on the surface and subsequently more algae formation. The appearance of the green algae indicated the bio-receptive character of the concrete panels ( Figure 12).

Bio-colonization Results
After the trial period, the first 6 weeks, the panels were kept in a horizontal position to allow them to remain wet for a longer time and create the required moist conditions for moss growth. Around the fourth week, small patches of green algae were seen on the surface of Panel 2, while the others remained unchanged as dark brown. During the six-week, bright green layers of algae had grown in the central alcoves of Panel 2, while light patches were also seen in Panels 1, 3 and 4, mostly around their central areas, due to the higher concentration of water spraying along this region. Among all the panels, the macro geometry of Panel 2 had the greatest depth, which allowed higher water retention on the surface and subsequently more algae formation. The appearance of the green algae indicated the bio-receptive character of the concrete panels ( Figure 12). Sustainability 2021, 13, x FOR PEER REVIEW an increase in the uptake of nutrients [20]. This stage is the early colonizing phase fungi and spores before the appearance of moss ( Figure 11). Figure 11. Fungus growth on the panels (symbiosis).

Bio-colonization Results
After the trial period, the first 6 weeks, the panels were kept in a horizontal p to allow them to remain wet for a longer time and create the required moist conditi moss growth. Around the fourth week, small patches of green algae were seen surface of Panel 2, while the others remained unchanged as dark brown. During t week, bright green layers of algae had grown in the central alcoves of Panel 2, whi patches were also seen in Panels 1, 3 and 4, mostly around their central areas, due higher concentration of water spraying along this region. Among all the panels, the geometry of Panel 2 had the greatest depth, which allowed higher water retention surface and subsequently more algae formation. The appearance of the green alga cated the bio-receptive character of the concrete panels ( Figure 12).  At the end of week 6, all the panels were placed in an upright position, to observe the role of geometry on water flow movement on the surface of the panels. After a week, the green patches on the panels appeared lighter and the panels seemed drier than before. The sudden change was assumed to be for two reasons, firstly, around mid-April, due to warmer outside temperature, the tropical greenhouse recorded a high temperature of 32 • C, resulting in rapid drying by evaporation, and secondly, the upright position allowed less time for the water to remain onto the surface due to the lack of deeper water catchment micro-grooves ( Figure 12).
In week 8, the panels were shifted to another region of the greenhouse, to maintain a temperature of 20-24 • C and slow down the drying effect. The progress was still found to be minimal. Thus, from week 10 onwards, the panels were laid horizontally again, to increase the water retention onto the surface. By the beginning of week 12, bright green patches of algae were seen on the textured surfaces. Panel 2, showed the highest growth, followed by Panel 1, owing to their textured macro-depths and continuous flow paths, allowing for trail growth in desired areas.
Therefore, from the above experiment, the following points need to be considered to enhance the moss-growing capacity onto the panels:

•
Twelve weeks is not enough to propagate moss growth on concrete surfaces. • Estimated time for algae appearance is 8-12 weeks and for moss growth it is 18-20 weeks.

•
The panels should be placed in a horizontal position until visible moss growth occurs. • Panels should have growth areas of connected macro-depths to allow continuous trails of growth and deep micro-grooves to allow sufficient water retention ability for a prolonged period when placed in a vertical position. • Further testing is required for mature moss-grown panels in vertical positions, extending the spraying duration to 2 min once daily, for a period of approx. 3-4 weeks.

Comparative Analysis
Based on the results of the design validation section, the moss-growing experiment and the water retention testing, this section has provided a comprehensive guideline for the design of bio-receptive concrete panels. Figure 13 is a comparative chart between the water retention/absorption capacity of the panels and the practical moss growth progress in the greenhouse. In terms of the water relations, Panels 3, 5 and 6 showed the best results. Panel 3 had higher surface water due to deeper alcove spaces and against-the-flow obstacles. Panels 5 and 6 showed high absorption compared to the other panels due to the absence of surface contaminants like silicone oil. For the designed panels, the silicone oil used in the molds for easy demolding left a residue layer over the concrete surface. The silicone oil, having high water-repellent capacity, hindered the absorption of water into the designed panels. In spite of the high absorption capacity, Panels 5 and 6 were not suitable for the ordered growth desired in this research. Even though Panel 3 showed the highest amount of surface water, water dripped from the alcove to the ridges due to the horizontal alignment of the macro-geometries (positioned against the main flow). The idea of steering in growth/no-growth areas did not work here, hence it hindered the ordered (pattern) growth requirement. The results from the greenhouse were obtained from week 12. Panel 2 showed the highest algae coverage on the surface, followed by Panel 1, due to the along the flow obstacles. The design features of Panel 2 provided a clear distinction between growth and no-growth areas, with its deeper and wider macro-geometry creating moist conditions on the surface in the horizontal position. However, a drastic drop in growth occurred when the panels were placed in vertical position. This was due to the quick drying of the surfaces from absence of enough water catchment areas. Though Panel 3 showed a good amount surface water, it failed to show adequate moss growth due to limited growth areas and insufficient micro-groove presences. areas, with its deeper and wider macro-geometry creating moist conditions on the surfa in the horizontal position. However, a drastic drop in growth occurred when the pan were placed in vertical position. This was due to the quick drying of the surfaces fro absence of enough water catchment areas. Though Panel 3 showed a good amount surfa water, it failed to show adequate moss growth due to limited growth areas and insu cient micro-groove presences. According to the analysis of the results, the presence of water was found to be esse tial for growth, which can be greatly influenced through geometry. The surface roughn According to the analysis of the results, the presence of water was found to be essential for growth, which can be greatly influenced through geometry. The surface roughness or topography was found to be a relevant influencing material characteristic in comparison to its porous quality. This was evident from the high absorption capacity of Panel 5 and Panel 6, with the lowest bio-colonization rate among all of the panels. As it can be seen in Figure 14, in the designed panel, a geometrical combination of macro-depth with deep micro-grooves along a larger surface area can allow better water catchment, while welldefined 'along the flow' macro-geometry can redirect water to growth areas, which can subsequently enhance the bio-receptive quality of the concrete panels.
Sustainability 2021, 13, x FOR PEER REVIEW 17 of 2 or topography was found to be a relevant influencing material characteristic in compar son to its porous quality. This was evident from the high absorption capacity of Panel and Panel 6, with the lowest bio-colonization rate among all of the panels. As it can b seen in Figure 14, in the designed panel, a geometrical combination of macro-depth wit deep micro-grooves along a larger surface area can allow better water catchment, whil well-defined 'along the flow' macro-geometry can redirect water to growth areas, whic can subsequently enhance the bio-receptive quality of the concrete panels.   Figure 15 provides a feedback loop by highlighting the pros and cons of the geometrical features of the panels that created the maximum positive impact in water absorption/retention on the surface. Features like continuous along-the-flow obstacles in flowing/alternating rhythms helped to direct water to growth areas by prolonging the flow path. Greater macrodepth provided the moist condition to promote cushion growth and deeper micro-grooves helped to increase surface water catchment and gather nutrients. A combination of these positive geometric features arranged in the desired rhythm could create an ordered system that could help enhance the bio-receptive qualities in the vertical position. The problem that was encountered in the moss-growing experiment with positioning was the fast drying out of the panels due to the absence of the righ tion of geometric features, particularly deeper micro-grooves, greater surfac growth and along the flow macro-geometries to direct water to growth areas. F tical position, what has been learned is that the ability to retain water for a period, allows the water to slowly seep into the material and create the requi climate for the mosses to proliferate. Therefore, the following design guidelines of the geometric features have on the practical results, which can drive the bio-receptive potential of the conc for real-time facade application. Nevertheless, as discussed in the results sect oculated panels should be placed in a horizontal position for the initial matu mosses, which can greatly provide a push for better functioning of the geomet when tested vertically for facade applications.

Design Guidelines
Based on the geometrical analysis so far, a general guideline was provide surface geometries that can enhance the bio-receptive character ( Figure 16). T macro-geometry that is the most suitable is continuous 'along-the-flow' obsta obstacles arranged in a flowing/alternating rhythm can prolong the flow pat lengths and promote a continuous trail of growth. From the results of the lab periment, a maximum macro-depth of 20 mm with a H/W ratio of 0.2-0.3 c The problem that was encountered in the moss-growing experiment with the vertical positioning was the fast drying out of the panels due to the absence of the right combination of geometric features, particularly deeper micro-grooves, greater surface areas for growth and along the flow macro-geometries to direct water to growth areas. For the vertical position, what has been learned is that the ability to retain water for a prolonged period, allows the water to slowly seep into the material and create the required micro-climate for the mosses to proliferate. Therefore, the following design guidelines of the geometric features have been based on the practical results, which can drive the bio-receptive potential of the concrete panels for real-time facade application. Nevertheless, as discussed in the results section, the inoculated panels should be placed in a horizontal position for the initial maturing of the mosses, which can greatly provide a push for better functioning of the geometric features when tested vertically for facade applications.

Design Guidelines
Based on the geometrical analysis so far, a general guideline was provided to create surface geometries that can enhance the bio-receptive character ( Figure 16). The type of macro-geometry that is the most suitable is continuous 'along-the-flow' obstacles. These obstacles arranged in a flowing/alternating rhythm can prolong the flow path to varied lengths and promote a continuous trail of growth. From the results of the laboratory experiment, a maximum macro-depth of 20 mm with a H/W ratio of 0.2-0.3 can provide larger defined space for growth and mirror the cushion growth form of mosses in nature. Verified through CFD simulations, a 5 mm depth of micro-grooves with adjusted w/h ratios has been found to create greater water catchment through the increased surface area. In addition to these geometrical features, the absorption capacity of the material is also equally important, which must be adapted according to literature research on bio-receptive material composition. Therefore, these geometric guidelines can be utilized to create a bio-receptive facade, addressing its overall order and balance aesthetic character.
ainability 2021, 13, x FOR PEER REVIEW 19 of Figure 16. The design guidelines.

Conclusions
This research is a testimony that bio-receptive material can be geometrically artic lated to engineer a self-sustaining process and manipulate the organic growth of mos in an ordered system that is not only functionally viable but also aesthetically pleasing further change the perception of people towards bio-receptivity. The building mater chosen to be investigated was concrete, due to its inherent bio-receptive quality and t capacity to reduce its carbon footprint with a more sustainable and greener version. T research followed a top-down approach where, firstly, designs were developed based a literature review and field surveys, then the fabricated panels were validated throu practical experimentations and finally, a general design guideline was provided.
The presence of water is vital for the growth and development of mosses on sto surfaces. Through the experimentations, the surface roughness or topography was fou to be the most crucial feature to create the desired micro-climate for the bio-receptive ch acteristics of the concrete panels. For vertical application of the panels, the geometric f tures, like the deep micro-grooves, can create greater water catchment areas on the surfa in combination with continuous 'along-the-flow' macro geometries, which can help to rect the flow path, creating a clear distinction between growth and no-growth areas. F the efficient functioning of these geometric features, the initial maturing of the mos should be allowed in a horizontal position before its vertical application. In support these geometric features, the material properties of concrete are also equally important they influence the water absorption capacity of the concrete. The absorption quality of t surface was enhanced by using blast furnace slag cement and a high-water cement ra of 0.60 in combination with a lack of curing during the hardening process. Using the guidelines, countless options of surface morphology can be used to create a self-sustaini bio-receptive concrete facade panel.
The results of this research work clearly coincide with some of the previous resear works where the role of surface roughness or topography in bio-receptivity were esta lished, namely the work of Ottelé et al. and Huang et al. [15]. In the former, the geome of the top layer of the concrete panel showed an innovative way to foster plant growth

Conclusions
This research is a testimony that bio-receptive material can be geometrically articulated to engineer a self-sustaining process and manipulate the organic growth of mosses in an ordered system that is not only functionally viable but also aesthetically pleasing, to further change the perception of people towards bio-receptivity. The building material chosen to be investigated was concrete, due to its inherent bio-receptive quality and the capacity to reduce its carbon footprint with a more sustainable and greener version. The research followed a top-down approach where, firstly, designs were developed based on a literature review and field surveys, then the fabricated panels were validated through practical experimentations and finally, a general design guideline was provided.
The presence of water is vital for the growth and development of mosses on stony surfaces. Through the experimentations, the surface roughness or topography was found to be the most crucial feature to create the desired micro-climate for the bio-receptive characteristics of the concrete panels. For vertical application of the panels, the geometric features, like the deep micro-grooves, can create greater water catchment areas on the surface in combination with continuous 'along-the-flow' macro geometries, which can help to direct the flow path, creating a clear distinction between growth and no-growth areas. For the efficient functioning of these geometric features, the initial maturing of the mosses should be allowed in a horizontal position before its vertical application. In support to these geometric features, the material properties of concrete are also equally important as they influence the water absorption capacity of the concrete. The absorption quality of the surface was enhanced by using blast furnace slag cement and a high-water cement ratio of 0.60 in combination with a lack of curing during the hardening process. Using these guidelines, countless options of surface morphology can be used to create a self-sustaining bio-receptive concrete facade panel.
The results of this research work clearly coincide with some of the previous research works where the role of surface roughness or topography in bio-receptivity were established, namely the work of Ottelé et al. and Huang et al. [15]. In the former, the geometry of the top layer of the concrete panel showed an innovative way to foster plant growth on its surface, providing a multifunctional characteristic to a regular construction material. The experiment also provided a clear relationship between the properties of the material and the growth of vegetation on the surface [4]. In the latter, the role of grooves was shown to have a significant effect on the concentration of microbial attachment on the substrate, which is a great analogy to the role of micro-grooves in this research [15].

Limitations
Bio-receptivity is a natural process, and its growth occurs in an unpredictable and spontaneous manner over a prolonged time, typically taking several years. For the time constraint of the research project, an ideal (growth) condition was created in a greenhouse to accelerate and propagate moss growth on the designed concrete panels. The selfsustaining ability of the panels in terms of surface water retention was tested within the controlled setting, which was not necessarily an ideal replica of natural rainfall and moisture conditions. Thus, to control and ensure successful performance of such designed panels in the natural environment, further testing needs to be done in outdoor settings for an extended period, particularly considering their vertical application, before it can be brought into building practice.