Recent Discovery of Amaranthus palmeri S. Watson in Italy: Characterization of ALS-Resistant Populations and Sensitivity to Alternative Herbicides

• Externally hosted supplementary file 1
  Doi: 10.17632/zjwvrbtbyt.1
  Link: https://data.mendeley.com/datasets/zjwvrbtbyt/1
  Description: This repository contains all the electropherograms (ABI format chromatogram file, ab1) obtained by Sanger sequencing carried out for the paper entitled “Recent discovery of Amaranthus palmeri (Amaranthaceae) in Italy: characterization of ALS-resistant populations and sensitivity to alternative herbicides”. Three A. palmeri (Palmer Amaranth) populations were found in soybean fields in Italy, were ALS-resistance was suspected. Bioassay test revealed that all the three populations were cross-resistant to thifensulfuron-methyl and imazamox, due to a point mutation at position 574 of ALS. DNA of 15 plants per population, survived to the field dose of thifensulfuron-methyl, was extracted and primers AMA-2F (5’-TCCCGGTTAAAATCATGCTC-3’ / AMA-2R (5’-CTTCTTCCATCACCCTCTGT-3’) were used to amplify the region surrounding the position 574. Amplicons were purified and primers AMA-2R was used for Sanger sequencing. In order to facilitate data interpretation, the sequences have been renamed as: Population-code_plant#_CODONS CODONS might be W for tryptophan (wild type) and L for leucine (mutated). WL refers to heterozygous genotypes. Population codes are the same used in the paper. Sequences should be reverse complemented.

Milani, A.; Panozzo, S.; Farinati, S.; Iamonico, D.; Sattin, M.; Loddo, D.; Scarabel, L. Recent Discovery of Amaranthus palmeri S. Watson in Italy: Characterization of ALS-Resistant Populations and Sensitivity to Alternative Herbicides. Sustainability 2021, 13, 7003. https://doi.org/10.3390/su13137003