Assessing new biotechnologies by combining TEA and TM-LCA for an efficient use of biomass resources

: An e ﬃ cient use of biomass resources is a key element of the bioeconomy. Ideally, options leading to the highest environmental and economic gains can be singled out for any given region. In this study, to achieve this goal of singling out an ideal technology for a given region, biotechnologies are assessed by a combination of techno-economic assessment (TEA) and territorial metabolism life cycle assessment (TM-LCA). Three technology variations for anaerobic digestion (AD) were assessed at two di ﬀ erent scales (200 kW and 1 MW) and for two di ﬀ erent regions. First, sustainable feedstock availability for two European regions was quantiﬁed. Then, the environmental impact and economic potential of each technology when scaled up to the regional level, considering all of the region’s unique sustainably available feedstock, was investigated. Multiple criteria decision analysis and internalized damage monetization were used to generate single scores for the assessments. Preference for the technology scenario producing the most energy was shown for all regions and scales, while producing bioplastic was less preferable since the value of the produced bioplastic plastic was not great enough to o ﬀ set the resultant reduction in energy production. Assessing alternatives in a regional context provided valuable information about the inﬂuence of di ﬀ erent types of feedstock on environmental performance.


Introduction
One of the goals of the European Union (EU) is to stimulate the creation of a competitive low carbon economy that is able to provide a reduction of 80%-95% greenhouse gas (GHG) in Europe by 2050 [1]. Energy production is an important sector where changes can be made in order to reach this ambitious target. Shifting from fossil-based energy to renewable sources of energy can lead to GHG reductions, provided the value chains for the renewable energy sources can lead to better overall environmental performance. A careful evaluation of new renewable energy pathways has previously been recommended [2] and various studies have shown wide ranges for GHG emissions of renewable energy systems [3,4]. Moreover, and particularly relevant to biomass based renewable energy, in some cases lower GHGs are not accompanied by lower emissions of other environmentally concerning emissions, such as those contributing to eutrophication, acidification, and human/ecosystem may be built in an area, e.g., considering that 50% of the EU's biogas capacity is in Germany [7]. As has been pointed out by Bojesen [25] and colleagues, who estimated service areas for existing and future biogas plants in Denmark, the availability of feedstock in relation to plant location is an important aspect. An inadequate assessment of a plant's sourcing ability may lead to high operation costs from increased transport demand or inadequate sourcing of feedstock [25]. In turn, high transport distances may negate the environmental benefits brought about by biorefineries, as shown in Croxatto Vega et al. [26] which applied the territorial metabolism-LCA approach (TM-LCA) [27] and found distances of 50 km to be the upper limit.
This study performs a step-wise assessment starting from individual plant level and investigates the implementation potential of the PHB and AD-Booster technologies in two different plant scales. A techno-economic assessment (TEA) and LCA are carried out for this aim. The results from the TEA-LCA are used to structure implementation of the technologies at the regional level. The TEA relates the plant scale and processing capacity to capital expenditure (CapEx) and operational expenditure (OpEx) of the plant, and to the break-even prices of products. In the LCA, the environmental aspects of different technologies are quantified. The implementation of the two technologies is analyzed for two regions defined by the nomenclature of territorial units for statistics (NUTS) from Eurostat's definition of regions (NUTS2 regions): Bavaria, Germany and Veneto, Italy. We analyze the potential impacts of the two innovative technologies (PHB and AD-Booster) against the current level of biogas implementation for the regions. First, we use TEA to analyze the effect of scale on the economic potential considering relevant plant sizes. Concurrently, we provide a mass flow analysis for the regions to better understand the energetic potential of agricultural residues produced within the regions (i.e., both the residues already in use for biogas and not yet exploited) as well as the level of development of the biogas sector (i.e., installed capacity). Finally, we use the results from the TEA of each technology to perform a TM-LCA, which will be able to tell us the possible environmental improvements (or deterioration) potentials for the whole region, if all of the residues are processed with the new technologies. We place special attention on the repercussions for the farmer, especially from installation of large biogas plants, which can potentially monopolize biomass resources over a large area. Vice versa, we explore the possible needs and constraints for biogas developers in the two regions. In this way, we seek to explore new biotechnological implementation potentials from a stakeholder's perspective.

Plant Level Assessment
The potential of implementing new AD technology was analyzed at two different scales. Data was collected from two biogas plants: a 1 MW installed electric capacity plant in Veneto, Italy and a smaller 200 kW plant in Bavaria, Germany, hereafter referred to as "the farms". Both plants operate on a mixture of cow manure, crop residues, and maize silage (Table 1). Both plants valorize biogas in CHP units, which produce heat as a waste product. Both plants utilize the co-produced heat in the plant's operation and additionally, in the Bavarian case, the surplus heat produced is utilized in the district heating network for a nearby village [28].

Technology Description
Three technology scenarios were assessed. Conventional AD was chosen as the baseline and two emerging treatment processes that can be added to existing AD were assessed for the comparison. All technology scenarios are modelled with a biogas leak of 3% of the produced biogas [22]. The technology set ups are: AD, AD + Booster, and AD + PHB.

Anaerobic Digestion
Conventional AD was modelled using SuperPro Designer, following the details received from the farms (Figure 1). The feedstock is grinded before it enters the anaerobic digester. The anaerobic digester produces biogas and digestate. The AD model was populated with the most common stoichiometric equations governing anaerobic digestion in [29]. Internal electricity consumption for the whole process was 7.5% of produced electricity based on data obtained from the farms operating biogas plants. A methane content of 50% for the biogas and an electrical efficiency of 38% for the CHP unit was used, based on the received data, yielding a 1.9 kWh/m 3 of biogas. Internal thermal energy usage was assumed to be 40%. The methane content, electrical efficiency, energy content of the biogas, and internal heat use was equal in all technology scenarios.

Technology Description
Three technology scenarios were assessed. Conventional AD was chosen as the baseline and two emerging treatment processes that can be added to existing AD were assessed for the comparison. All technology scenarios are modelled with a biogas leak of 3% of the produced biogas [22]. The technology set ups are: AD, AD + Booster, and AD + PHB.

Anaerobic Digestion
Conventional AD was modelled using SuperPro Designer, following the details received from the farms (Figure 1). The feedstock is grinded before it enters the anaerobic digester. The anaerobic digester produces biogas and digestate. The AD model was populated with the most common stoichiometric equations governing anaerobic digestion in [29]. Internal electricity consumption for the whole process was 7.5% of produced electricity based on data obtained from the farms operating biogas plants. A methane content of 50% for the biogas and an electrical efficiency of 38% for the CHP unit was used, based on the received data, yielding a 1.9 kWh/m3 of biogas. Internal thermal energy usage was assumed to be 40%. The methane content, electrical efficiency, energy content of the biogas, and internal heat use was equal in all technology scenarios.

AD + Booster
The AD + Booster technology consists of an extra tank where the wet explosion technology is applied under high heat and pressure conditions [13]. The AD + Booster scenario ( Figure 2) was designed with information obtained from the technology developers [30]. In comparison to AD, the AD + Booster technology increases the conversion yield of cellulose to biogas from 52% to 88% and the conversion yield of hemicellulose to biomass from 75% to 98%. This scenario has an internal electricity consumption of 9.5% of produced electricity. On the other hand, the biogas yield is 12% to 16% higher than the AD scenario.

AD + PHB
In order to include a PHB producing section into an existing AD plant, a few extra pieces of equipment are necessary ( Figure 3). AD is split into two tanks, the first is of short retention time and is where the VFA are produced and rerouted for PHB production. After this step, a screw press and a filtration unit separate solid from liquid. The solid fraction is fed to the AD tank where it continues the regular AD process, while the liquid fraction goes into a series of bio-oxidation units where selection and accumulation occurs via the feast and famine method [15]. The bio-oxidation

AD + Booster
The AD + Booster technology consists of an extra tank where the wet explosion technology is applied under high heat and pressure conditions [13]. The AD + Booster scenario ( Figure 2) was designed with information obtained from the technology developers [30]. In comparison to AD, the AD + Booster technology increases the conversion yield of cellulose to biogas from 52% to 88% and the conversion yield of hemicellulose to biomass from 75% to 98%. This scenario has an internal electricity consumption of 9.5% of produced electricity. On the other hand, the biogas yield is 12% to 16% higher than the AD scenario.

Technology Description
Three technology scenarios were assessed. Conventional AD was chosen as the baseline and two emerging treatment processes that can be added to existing AD were assessed for the comparison. All technology scenarios are modelled with a biogas leak of 3% of the produced biogas [22]. The technology set ups are: AD, AD + Booster, and AD + PHB.

Anaerobic Digestion
Conventional AD was modelled using SuperPro Designer, following the details received from the farms (Figure 1). The feedstock is grinded before it enters the anaerobic digester. The anaerobic digester produces biogas and digestate. The AD model was populated with the most common stoichiometric equations governing anaerobic digestion in [29]. Internal electricity consumption for the whole process was 7.5% of produced electricity based on data obtained from the farms operating biogas plants. A methane content of 50% for the biogas and an electrical efficiency of 38% for the CHP unit was used, based on the received data, yielding a 1.9 kWh/m3 of biogas. Internal thermal energy usage was assumed to be 40%. The methane content, electrical efficiency, energy content of the biogas, and internal heat use was equal in all technology scenarios.

AD + Booster
The AD + Booster technology consists of an extra tank where the wet explosion technology is applied under high heat and pressure conditions [13]. The AD + Booster scenario ( Figure 2) was designed with information obtained from the technology developers [30]. In comparison to AD, the AD + Booster technology increases the conversion yield of cellulose to biogas from 52% to 88% and the conversion yield of hemicellulose to biomass from 75% to 98%. This scenario has an internal electricity consumption of 9.5% of produced electricity. On the other hand, the biogas yield is 12% to 16% higher than the AD scenario.

AD + PHB
In order to include a PHB producing section into an existing AD plant, a few extra pieces of equipment are necessary ( Figure 3). AD is split into two tanks, the first is of short retention time and is where the VFA are produced and rerouted for PHB production. After this step, a screw press and a filtration unit separate solid from liquid. The solid fraction is fed to the AD tank where it continues the regular AD process, while the liquid fraction goes into a series of bio-oxidation units where selection and accumulation occurs via the feast and famine method [15]. The bio-oxidation

AD + PHB
In order to include a PHB producing section into an existing AD plant, a few extra pieces of equipment are necessary ( Figure 3). AD is split into two tanks, the first is of short retention time and is where the VFA are produced and rerouted for PHB production. After this step, a screw press and a filtration unit separate solid from liquid. The solid fraction is fed to the AD tank where it continues the regular AD process, while the liquid fraction goes into a series of bio-oxidation units where selection and accumulation occurs via the feast and famine method [15]. The bio-oxidation equipment, in SuperPro Designer, was populated with stoichiometric equations obtained from the Sustainability 2020, 12, 3676 5 of 35 technology developers. Finally, PHB can be extracted using sodium hypochlorite and a final filtration step recovers a crude PHB. In comparison to AD, this scenario has an internal electricity consumption of 15% of produced electricity and a biogas yield from 24% to 30% lower than AD.
Sustainability 2020, 12, 3676 5 of 41 equipment, in SuperPro Designer, was populated with stoichiometric equations obtained from the technology developers. Finally, PHB can be extracted using sodium hypochlorite and a final filtration step recovers a crude PHB. In comparison to AD, this scenario has an internal electricity consumption of 15% of produced electricity and a biogas yield from 24% to 30% lower than AD.

Crops
Primary production amounts and land cover of individual crops was obtained from the Eurostat database (apro_cpnhr, [31]) for the two NUTS2 regions. As much as possible, the most recent data on production statistics was used, for the period of 2008-2018. For Veneto, data coverage on crops by Eurostat was very incomplete. Thus, it was preferable to use data from the National Italian Institute of Statistics (ISTAT) [32], where data is available for the whole period at the NUTS2 level. The production yield (production amounts divided by area of production) was then averaged over the period to derive an average production per year for the regions. Residue:crop ratios where then applied to the production yield to derive a total annual amount of residues for each crop. A list of the residue:crop ratios (Table 2), as well as grouping of Eurostat categories used are provided (Table A1, in Appendix A). For the most part, it was assumed that only residues are used for AD (or AD + innovation), with the exception of energy crops where the whole plant is assumed to enter digestion. Energy crops included are maize silage, green maize, and sorghum. Only crops which are most commonly considered for biogas operation were included in the study; we excluded horticultural crops that do not typically serve a purpose in AD.
Because wine grapes are an important crop in the Veneto region, they are also present, though to a smaller extent in the crop mix of Bavaria. Amounts of winery pomace were also taken into account as potential AD feedstock. Data on wine production was obtained from Eurostat (vit_t1, vit_an5, vit_an7, [33,34]) for both regions and the data for Veneto was checked against ISTAT data. The period for Eurostat wine data differed slightly and included the years 2001-2009, and 2015. The amount of pomace produced was estimated based on [35], which reports a 25% conversion rate from grapes to pomace.
After obtaining total crop residue amounts for the region, it is necessary to estimate a technical and sustainable potential for collection of the residues. The technical potential may potentially exclude the share of residues which is too difficult to collect, as well as the share that has known competing applications (Table 3). For example, this is the case for straw from cereal production, which is typically used for bedding and feed for cattle and other animals [36]. Sustainable potential collection, on the other hand, takes into consideration soil fertility. Residues may, for example, be left

Crops
Primary production amounts and land cover of individual crops was obtained from the Eurostat database (apro_cpnhr, [31]) for the two NUTS2 regions. As much as possible, the most recent data on production statistics was used, for the period of 2008-2018. For Veneto, data coverage on crops by Eurostat was very incomplete. Thus, it was preferable to use data from the National Italian Institute of Statistics (ISTAT) [32], where data is available for the whole period at the NUTS2 level. The production yield (production amounts divided by area of production) was then averaged over the period to derive an average production per year for the regions. Residue:crop ratios where then applied to the production yield to derive a total annual amount of residues for each crop. A list of the residue:crop ratios (Table 2), as well as grouping of Eurostat categories used are provided (Table A1, in Appendix A). For the most part, it was assumed that only residues are used for AD (or AD + innovation), with the exception of energy crops where the whole plant is assumed to enter digestion. Energy crops included are maize silage, green maize, and sorghum. Only crops which are most commonly considered for biogas operation were included in the study; we excluded horticultural crops that do not typically serve a purpose in AD.
Because wine grapes are an important crop in the Veneto region, they are also present, though to a smaller extent in the crop mix of Bavaria. Amounts of winery pomace were also taken into account as potential AD feedstock. Data on wine production was obtained from Eurostat (vit_t1, vit_an5, vit_an7, [33,34]) for both regions and the data for Veneto was checked against ISTAT data. The period for Eurostat wine data differed slightly and included the years 2001-2009, and 2015. The amount of pomace produced was estimated based on [35], which reports a 25% conversion rate from grapes to pomace.
After obtaining total crop residue amounts for the region, it is necessary to estimate a technical and sustainable potential for collection of the residues. The technical potential may potentially exclude the share of residues which is too difficult to collect, as well as the share that has known competing applications (Table 3). For example, this is the case for straw from cereal production, which is typically used for bedding and feed for cattle and other animals [36]. Sustainable potential collection, on the other hand, takes into consideration soil fertility. Residues may, for example, be left on agricultural fields to uphold the organic matter content of the soil and protect it from excessive erosion. Sustainable potential collection factors typically used in the literature vary from around 10% to 50% of the most common types of residues, i.e., excluding pomace and energy crop [37], and it has been shown that residue removals above 50% may negatively affect soil organic carbon storage [38]. Nearly all studies [11,16,[19][20][21]36,39] that evaluate biomass potential for bioenergy purposes apply some sort of technical/sustainable collection factor, yet many of these studies do not report the actual values used or leave values out. We report all the values used in Table 2, since this is one of the most important determinants of potential for biomass utilization.

Manure
Animal production data was obtained from the Eurostat database (agr_r_animal) for bovine, swine, sheep, and goats for the period of 2008-2018. At the NUTS2 level, it is possible to obtain data for the number of animals in thousand heads. It is then necessary to estimate the amounts of manure excreted by the different types of animal, which varies also with their age (dairy cows, calves, sows, piglets, etc.). Values of manure production are calculated using the methodology detailed in [43] following the definitions for the various animals in [44]. The values are reported in Appendix A, in Table A2. Poultry production is not reported in the above-mentioned database, thus it was necessary to use the ef_lsk_main Eurostat database, which reports livestock units (LSU) for poultry for the years 2005,2007,2010,2013, and 2016 at the appropriate regional level. This was the best available data for poultry at the NUTS2 level. LSU values were converted to poultry heads, following the methodology outlined in [43].
Similarly to crop residues, a technical potential was considered for animal manures. Here, for cattle, the potential collectable manure was estimated based on the type of housing and rearing. Since European regulation on organic production of agricultural products specifies that organic "livestock should have permanent access to open air areas" in most cases [45] and that there shall be a connection between land management by the use of manure, i.e., meaning that organic production must maintain the fertility of soil by applying cover crops, green manures or organic livestock manure, it was assumed that manure could only be collected in the harsh winter months (at most) from organic cattle farms [46]. The estimate for housing types was derived from the Farm Structure Survey (FSS) [47] carried out in 2010, since more recent FSS could not be located. The types of housing were assumed to stay proportionally equal to the values in 2010, though after taking into consideration the growth in the organic farming sector for cattle rearing. Data on the share of organic livestock was obtained from statistical data summarized by Eurostat at the national level [48]. For animals other than cattle, the share of organic production was disregarded since the share is very low (<1% of animals) [48]. Manure collection factors are given in Table A3 of Appendix A, for all animals and various types of housing.

Installed AD Capacity
Already installed AD capacity has to be considered when assessing additional potential implementation in the regions. Regional data on biogas installation was collected from various sources. In Veneto, a total of 220 biogas plants were in operation by 2018, of which 89% were considered agricultural plants, i.e., treating crop residues, energy crops and animal manures [49]. By contrast, 2566 plants were installed in Bavaria by 2019 [50], of which 93% were considered agricultural AD [7], while the rest were landfill gas and sewage gas. A breakdown of types of installed capacity (scale) was obtained from a census of installed plants [51] in 2011 in the Veneto region. It was assumed that installation continued in the same fashion through to 2018, with a preference for plants of capacity slightly lower than 1MW, due to an all-encompassing subsidy [52]. For Bavaria, data obtained was detailed down to city/rural district level, which made it possible to use average capacity to determine the scale breakdown of installed capacity. The types of capacity installed estimated for Veneto and Bavaria are shown in Table 4.

Regional Energetic Potential
The methane potential of various feedstocks (Table A4, Appendix A) was used to derive the quantities of feedstock currently being processed by the already installed AD capacity in each region. Since it was not possible to obtain specific data on precisely what types of feedstock are used at the NUTS2 level, statistics on the manure to crop share processed in AD were scaled down from national to regional level. For Germany, feedstock inputs for agricultural biogas plants are on average 45% manures and 55% crop material [28], while in Veneto the mix is on average 55% manures and 45% crop material [53]. A CHP electrical efficiency of 38% and a value of 9.97 kWh per liter of methane (CH 4 ) were assumed. The capacity installed in each region corresponds to 137 MW in Veneto and 1237 MW in Bavaria. Taking account for the installed capacity, the average mix of manure and crop material present in each region is then used to estimate more precisely the feedstock already used in AD. The final available potential can then be calculated by taking the total agricultural feedstock produced and subtracting competing applications for animals, soil organic matter and already installed capacity.

TEA Method
TEA of the different technologies, utilizing different feedstock mixes was carried out. Financing costs, maintenance and plant overhead costs, labor related costs, and feedstock costs were aspects Sustainability 2020, 12, 3676 8 of 35 considered for the TEA. For all scenarios, it was assumed that the AD plant has a productivity of 8760 hours per year.
The CapEx of the AD plants were estimated using a CapEx of M€ 4 for a 1 MW plant complete with AD, H 2 S washer, and generator as a reference, which scales with a power of 2/3 to the electricity output [28,54]. The AD + Booster technology requires extra equipment for the separation and heat treatment, but it also reduces the required hydraulic retention time and therefore the required equipment size of the digester. Based on expert knowledge, it was assumed that regarding the CapEx these aspects equalize and therefore the CapEx of the AD + Booster scenarios is equal to that of the AD plants. The PHB production requires extra equipment for separation, filtration, selection, accumulation, and concentration. Based on expert knowledge, the CapEx for the AD + PHB scenarios was estimated to be 25% higher compared to that of the AD plant.
The financing costs were based on an amortization of the CapEx over 10 years with no interest. Maintenance, tax, insurance, rent, plant overhead, environmental charges, and royalties were assumed to be 10% of the CapEx per year [55,56].
The AD plants were assumed to have a high level of automation, thus, the labor related costs for a 1 MW plant are based on a 1 shift position. Assuming that an operator earns a salary of €18/h and including costs for supervision (+25%), direct salary overhead (+63%), and general plant overhead (+122%) [55], resulted in total labor related costs of k€ 500/y. For the 200 kW plant the labor related costs were divided by five, assuming farm personnel are available part-time. As the PHB production requires a number of extra unit operations and produces an extra product, the labor related costs were assumed to be 50% higher.
The feedstock costs including raw material, and handling and transportation costs are shown in Table 5. The costs for the different types of manure were estimated based on short distance transport costs of manure of €1/ton wet weight (WW) and thereby depend on the dry weight (DW) content of each feedstock. Grass and corn silage were assumed to be produced close to the AD plant and costs were estimated based on [57] and [58]. The costs for wheat straw, corn stover, and soybean straw were based on baling and transportation costs. The costs for vine shoots were based on harvesting and transportation costs. The costs for grape pomace, sugar beet pulp, and grain were based on [58]. Based on the total costs, the break-even prices for electricity and crude PHB were calculated. In the scenarios in which crude PHB is produced, the break-even price of electricity is equal to the regular AD scenario. The break-even prices were compared to selling prices of electricity and PHB ( Table 6). As in the AD + PHB scenarios a concentrated crude PHB is produced, extra required purification costs were included. For comparison between the economic performance of each scenario, the required subsidy, i.e., the difference between the selling prices and the break-even prices was calculated.

LCA Method
LCA is a standardized methodology governed by international standards and guidelines [8]. Among these, the ILCD handbook offers detailed guidance on how to carry out LCAs in accordance with the definitions set out by the European guidelines [63]. Using this guidance, the study at hand is considered a situation A "micro-level decision support", since structural changes are not foreseen to occur in the background system, due to the small share of biogas in the overall context of renewable energy. Thus, average mixes were used for the background system and replacement of substituted products. Where co-products are produced, such as in the case of AD + PHB, system expansion is used. The same was done for heat, which is produced as a by-product when biogas is burned in a CHP unit. Though in the latter no credits were awarded in the Veneto region for the produced heat, since this is not yet valorized in Italy [51], apart from what is used for own consumption from operation of the plant. In Germany, the situation is slightly different, and thus, a credit was given to the co-produced heat at a rate of 0.52 kwh heat/kwh electricity, based on the amount of heat utilized at national level [28].
Residue feedstocks that are presently not typically valorized, apart from biogas production, come into the system burden free, since the burden of production was allocated solely to the main product. This is the case for animal manures, pomace and vine shoots. However, for energy crops, the full burden of production was taken into account, i.e., maize silage, grain and grass silages. For agricultural residues currently valorized in the market, such as sugar beet pulp, corn stover, and soybean straw, the burden of production was distributed by economic allocation, while for wheat straw an existing Ecoinvent process was used. The allocation key is shown in Table 7. In order to visualize the benefit of digesting manure, emissions from storing manure have been included in the assessment. A period of 50 days of manure storage, minus two weeks of unavoidable storing to account for losses and manure in housing units, is avoided by instead treating the manure with the technology scenarios. The quantity of avoided methane is directly proportional to the quantity of manure available in the region or the amount of manure is the feedstock mix. Values used for the calculation are included in Table A5 of Appendix A.
The product system modelling software OpenLCA [68] was used for the modelling and subsequent analysis, utilizing the Ecoinvent v3 database [69]. ReCiPE Hierarchist (H) [70] was chosen as the impact assessment method, and results were generated at midpoint and endpoint. The time horizon for calculation of impacts is 100 years from point of emission.

Plant Level
The functional unit (FU) at plant level is the treatment of 1 ton of feedstock of local characteristics, defined in Table 1 for each plant. Biogas is burned in a CHP, producing heat and electricity. Electricity substitutes the production mix corresponding to the geographical location of each biogas plant. Heat utilization was modeled as substituted district heat for the 200 kW Bavarian plant based on their data, while there is no heat utilization for the industrial size plant in Veneto. PHB production offsets average global thermoplastic production (Table A6, Appendix A).

Regional Level
The FU at NUTS2 level is the treatment of all the AD compatible feedstock defined through the mass flow analysis of available potential for each region (see Section 3.1). An energetic cut off of 1% was applied, so that feedstocks contributing less than 1% of total energetic potential of all feedstock in the region were left out. To simplify matters further, partly due to results from the TEA, the regional assessment was done for plants of industrial size, i.e., 1000 kW for both locations, processing a feedstock mix corresponding to the regional availability, which is defined in the regional feedstock availability assessment. Transport for the regional and plant level assessments was included as 1 km of feedstock transport, and other distances were tested in a sensitivity analysis.
A second sensitivity analysis was also included. The energy grid of each location was replaced with a theoretical future green energy mix, in order to observe the effect of changing energy grids through time. This follows best practices for including partially dynamic LCA in systems with a long service life [71].

Interpretation of Environmental Impacts
In order to interpret the results, several methods were used. Because of political importance as well as ease of understanding, GHG emissions were used as a proxy for environmental impacts in some discussion, though due to the potential issues with only using GHG emissions, e.g., burden shifting [72], other interpretation methods are were also used. In particular, two methods were used: the first is a monetization of environmental impacts based on endpoint damages [73] and the second uses a form of multiple criteria decision assessment called technique for order of preference by similarity to ideal solution (TOPSIS) [74], utilizing the implementation method ArgCW-LCA [75].
In the first of these two methods, monetization and ReCiPe endpoint damages [76,77] are used to calculate the external costs of the implementation of a given technology at a given scale or region. This was done through two methods. The first, for ecosystem damages, is based on budget constrained ability to pay, which is used to derive a valuation for species years (Species.Yr) gained or lost [78], as this is suggested as the least uncertain method for this valuation [79]. For that valuation, 65,000 USD 2003 per Species.Yr was utilized. In order to evaluate the disability adjusted life year (DALY) loss or gain, a value from Dong et al., who assessed a number of different methods, was utilized [80]. The valuation derived in these different methods varies significantly, on the range of 1 to 2 orders of magnitude. Therefore, here we used the average of these values, 110,000 USD 2003 per DALY, which is also in line with the value derived from budget constraint monetization [78], which again should have the least uncertainty. Since resource scarcity endpoint damages are already expressed in monetary terms, no further interpretation is necessary.
In the second method used for deriving a single score, based on the ArgCW-LCA method [75], ReCiPe midpoint environmental impacts [76] along with a valuation of required subsidy for profitability to represent the economic impacts were used as the input criteria for TOPSIS utilizing weighting based on what Sohn et al., describe as a context weighting factor (CWF) [75]. Per a suggestion from the ArgCW-LCA method, as there was no specific stakeholder group present, the stakeholder perspective element was omitted from the method application. For this application, normalization for an average European person year emission was used [81]. Thus, weighting of the environmental impacts is derived, as described in the ArgCW-LCA method, by taking an average of two values: the average of the normalized midpoint impacts for impact category 'i' amongst all assessed scenarios, and the difference of the minimum and maximum normalized impacts for impact category 'i' amongst all assessed scenarios. This accomplishes two things: (1) taking the average of the normalized impacts scales the importance of emissions of the system to status quo emissions and (2) taking the difference between the maximum and minimum normalized impacts is to scale relative to the ability for choosing amongst the available alternatives to cause significant change in status quo emissions. This was completed for all impact categories resulting in the CWF for the environmental impacts. Economic impacts were ascribed a range of weights relative to the sum of weighting given to environmental impacts ranging from 10%-90%. The system was also run using equal weights for all criteria as a point of comparison to the context weighted and the other single score results.

Regional Feedstock Availability and Potential Bioenergy Production
A complete table of the sustainable/technical feedstock potential is presented in Appendix B, Table A7, for Bavaria and Veneto. These amounts were used for the TEA-LCA as the regional feedstock mix, though with a 1% cut-off based on the energetic potential of the feedstock.
When graphed on a % wt basis (Figure 4), a relatively large proportion of production of energy crops is evident in Bavaria. Both regions are rich in cattle manure and have a noteworthy amount of swine manure. After energy crops, the most abundant residues are cereal straw for Bavaria and sugar beet straw and soybean residues for Veneto. The regions notably differ from each other, in particular with regard to the production of certain crops, for example sugar beet, soya and grapes. The grapes, represented by pomace, are much more prominent in the Veneto region.  Nevertheless, in energy terms, the potential of the feedstock mix is different than the availability based on mass, mostly due to the poor methane potential of some of the feedstocks. Without subtracting the feedstock that is already being used in the installed capacity of these regions, the energetic potential (based on electrical power) is seen in Figure 5. The largest share of potential is dominated by different feedstocks in the two regions. In Bavaria, the largest share can be obtained from energy crops, while in Veneto the largest share can come equally from cattle manure or energy crops. As a rough estimate, 153 PJ and 38 PJ remain as unexploited feedstock. This represents 31% and 54% of the total available feedstock potential, in Bavaria and Veneto, respectively, which is estimated as described in Section 2.3.4. However, for the LCA, all of the feedstock in the region was assumed to be utilized by the technologies, since in theory biogas plants can be retrofitted with the additional equipment needed for implementation of the AD + Booster technology and PHB production. In comparison to Veneto, Bavaria has a much larger share of energy crops, mainly maize silage. This greater share of energy crops is explained by feed incentives given to biogas plants using energy crops in Germany during 2004-2008 [82]. Although maize production has been capped by several German rulings, from 60% by mass input in 2014 lowered to 50% in 2018 and 44% by 2021, the combination of a high animal density and fodder production means that growing of maize has increased exponentially with unintended consequences, such as increasing land prices [22,82]. In Veneto, the feedstock mix exhibits more variability and the expansion of energy crops has not been as dramatic. This may likely be due to the Biogasdoneright™ concept promoted by the Italian Biogas Association, which originates in northern Italy, under which sequential crop cultivation is practiced, where the primary food crop goes to its intended purpose, and a secondary cover crop serves as feedstock for biogas plants [83].
Nevertheless, in energy terms, the potential of the feedstock mix is different than the availability based on mass, mostly due to the poor methane potential of some of the feedstocks. Without subtracting the feedstock that is already being used in the installed capacity of these regions, the energetic potential (based on electrical power) is seen in Figure 5. The largest share of potential is dominated by different feedstocks in the two regions. In Bavaria, the largest share can be obtained from energy crops, while in Veneto the largest share can come equally from cattle manure or energy crops. As a rough estimate, 153 PJ and 38 PJ remain as unexploited feedstock. This represents 31% and 54% of the total available feedstock potential, in Bavaria and Veneto, respectively, which is estimated as described in Section 2.3.4. However, for the LCA, all of the feedstock in the region was assumed to be utilized by the technologies, since in theory biogas plants can be retrofitted with the additional equipment needed for implementation of the AD + Booster technology and PHB production. Nevertheless, in energy terms, the potential of the feedstock mix is different than the availability based on mass, mostly due to the poor methane potential of some of the feedstocks. Without subtracting the feedstock that is already being used in the installed capacity of these regions, the energetic potential (based on electrical power) is seen in Figure 5. The largest share of potential is dominated by different feedstocks in the two regions. In Bavaria, the largest share can be obtained from energy crops, while in Veneto the largest share can come equally from cattle manure or energy crops. As a rough estimate, 153 PJ and 38 PJ remain as unexploited feedstock. This represents 31% and 54% of the total available feedstock potential, in Bavaria and Veneto, respectively, which is estimated as described in Section 2.3.4. However, for the LCA, all of the feedstock in the region was assumed to be utilized by the technologies, since in theory biogas plants can be retrofitted with the additional equipment needed for implementation of the AD + Booster technology and PHB production.

TEA Results
Based on the technical description of the different technologies and the different feedstock compositions, the flow sizes, flow compositions, production of electricity, heat, and crude PHB were estimated. Linking these process parameters to the economic parameters results in the TEA in Table 8.
In all scenarios, the financing, maintenance, labor-related, and feedstock costs are in the same order of magnitude. The contributions of these cost aspects to the total cost vary between 19% and 34%. The small-scale scenarios have, relative to annual production, a larger CapEx compared to the industrial scale, therefore financing and maintenance costs increase the break-even prices for the small-scale scenarios. This results in a break-even price are 34% higher for electricity and 27% higher for crude PHB for the small-scale scenarios, compared to the industrial scale scenarios. As all cost aspects are in the same order of magnitude, the extra required labor in the AD + PHB scenarios results in a significant contribution to the total costs. Logically, the extra labor related costs increase the break-even price of crude PHB. Compared to the feedstock costs of the studied plants, the regional level feedstock in both Bavaria and Veneto have a slightly higher contribution to the costs and to the break-even prices. In the Bavaria scenarios, the revenues of the thermal energy cause a reduction to the break-even prices of 8% for the small scale and 6% for the industrial scale, relative to scenarios that do not utilize the thermal energy.
For the 1 MW AD plant scenarios the average estimated break-even price for electricity is €0.22/kWh. For the AD + Booster scenarios, the average estimated break-even price for electricity is €0.19/kWh, a reduction of 12% in comparison to AD alone. Using the break-even for electricity of regular AD in the AD + PHB scenarios results in an estimated break-even price for crude PHB in the range €4.3/kg to €4.7/kg. When the purification costs of €1.8/kg are included, the break-even price range for PHB is in the range €6.1/kg to €6.5/kg. Due to the difference between market price and the break-even prices, as outlined in Section 2.4 (Table 6), it is clear that both electricity and PHB require large subsidy contributions to be profitably produced in AD plants. Relative to their respective market prices, the required amount of subsidy for the production of PHB is smaller compared to the subsidy for the production of electricity. Nevertheless, the production of PHB requires the co-production of electricity (Table 8).

Midpoint Results
Results were obtained both at midpoint and endpoint level, using the ReCiPE 2016 (H) LCIA methodology. The results were internally normalized and ranked relative to the best-performing technology scenario. Midpoint level results for both regions and scales showed, for the most part, the same technology preference, pointing to AD + Booster as the best performer across impact categories (ICs), followed by AD and lastly AD + PHB. In the Veneto region, slightly more variation is observed across impact categories ( Figure 6) and AD + PHB can at times be the best performer, as seen in the Ionizing Radiation, Land Use, and the Mineral Resource Scarcity ICs. The importance of this variation was tested with TOPSIS and is discussed further in Section 3.4.  Midpoint results for the two farms assessed the small scale 200 kW farm in Bavaria and the 1000 kW farm in the Veneto region showed identical preference to the regional assessment when ranked within geographical location. However, more rank switching is observed when ranking is done across scales; this is explored further and discussed in Section 3.4., where rank reversal is checked thoroughly for both regional and scale assessments.  (Tables A8-A11).

Global Warming
As mentioned previously, global warming potential (GWP) shows the same technology preference as other ICs, with AD + Booster performing better than AD, which in turn performs better than AD + PHB. Looking at the contribution to GWP from the various elements that make up the system, it is possible to understand this preference. As can be seen in Figure 4, the higher energy production of the AD + Booster induces a higher electricity offset, which is largely responsible for the technology preference exhibited by the results. It is also evident that the offset for substituting plastic in the market for the AD + PHB options is very moderate and occurs on account of lower energy production, resulting overall in the lowest GWP savings out of all technology options. Figure 4 also shows the difference between the two regions on a per ton feedstock mix basis. An important difference can be observed in the crop mixture used for each region, where it is evident that Bavaria uses a more burdensome mix than Veneto. Other than crop differences, methane leaks from the facilities, here assumed to be 3% of the biogas produced, is an important source of GHGs. This is Midpoint results for the two farms assessed the small scale 200 kW farm in Bavaria and the 1000 kW farm in the Veneto region showed identical preference to the regional assessment when ranked within geographical location. However, more rank switching is observed when ranking is done across scales; this is explored further and discussed in Section 3.4., where rank reversal is checked thoroughly for both regional and scale assessments.  (Tables A8-A11).

Global Warming
As mentioned previously, global warming potential (GWP) shows the same technology preference as other ICs, with AD + Booster performing better than AD, which in turn performs better than AD + PHB. Looking at the contribution to GWP from the various elements that make up the system, it is possible to understand this preference. As can be seen in Figure 4, the higher energy production of the AD + Booster induces a higher electricity offset, which is largely responsible for the technology preference exhibited by the results. It is also evident that the offset for substituting plastic in the market for the AD + PHB options is very moderate and occurs on account of lower energy production, resulting overall in the lowest GWP savings out of all technology options. Figure 4 also shows the difference between the two regions on a per ton feedstock mix basis. An important difference can be observed in the crop mixture used for each region, where it is evident that Bavaria uses a more burdensome mix than Veneto. Other than crop differences, methane leaks from the facilities, here assumed to be 3% of the biogas produced, is an important source of GHGs. This is worth noting, as it can diminish the savings intended by these technologies. On the other hand, an important savings is attained by degassing animal manures, which would otherwise sit in storage facilities for a longer period producing methane that would be released to the atmosphere. This benefit can be seen in Figure 7 as the "methane offset storage" and is higher for Veneto due to the higher availability of animal manures on a %wt basis in this region. worth noting, as it can diminish the savings intended by these technologies. On the other hand, an important savings is attained by degassing animal manures, which would otherwise sit in storage facilities for a longer period producing methane that would be released to the atmosphere. This benefit can be seen in Figure 7 as the "methane offset storage" and is higher for Veneto due to the higher availability of animal manures on a %wt basis in this region.  Figure 8A,B, shows total GWP savings for both Veneto and Bavaria, respectively. As a total, the Bavaria region is capable of obtaining GWP savings 7.4, 7.7, and 5.4 times higher than in the Veneto region for AD, AD + Booster, and AD + PHB, respectively, on an annual basis. This is explained in part by the scale of the regions, feedstock density of the regions, as well as the energy density of each feedstock employed in the mix. While Veneto is also the smaller of the two regions, the lower GWP savings are partly due to an average 25% lower feedstock mass production per area relative to Bavaria. Moreover, the regional feedstock mix in Bavaria contains ca. 7% more crops and crop residues, among which maize silage is a prominent one, whilst Veneto contains ca. 7% more animal manures, which have a low methane/VFA productivity. The feedstock mix of Bavaria results in a higher electricity offsets, even though its feedstock mix contains a higher share of primary production (1st Generation) feedstock, i.e., maize silage, rather than secondary production such as straw. In addition, the utilization of waste heat in the Bavarian system for district heating gives an extra considerable impact offset to the region. If the heat were to be utilized in Veneto, then an extra 23%-25% savings in GWP could be attained there.  Figure 8A,B, shows total GWP savings for both Veneto and Bavaria, respectively. As a total, the Bavaria region is capable of obtaining GWP savings 7.4, 7.7, and 5.4 times higher than in the Veneto region for AD, AD + Booster, and AD + PHB, respectively, on an annual basis. This is explained in part by the scale of the regions, feedstock density of the regions, as well as the energy density of each feedstock employed in the mix. While Veneto is also the smaller of the two regions, the lower GWP savings are partly due to an average 25% lower feedstock mass production per area relative to Bavaria. Moreover, the regional feedstock mix in Bavaria contains ca. 7% more crops and crop residues, among which maize silage is a prominent one, whilst Veneto contains ca. 7% more animal manures, which have a low methane/VFA productivity. The feedstock mix of Bavaria results in a higher electricity offsets, even though its feedstock mix contains a higher share of primary production (1st Generation) feedstock, i.e., maize silage, rather than secondary production such as straw. In addition, the utilization of waste heat in the Bavarian system for district heating gives an extra considerable impact offset to the region. If the heat were to be utilized in Veneto, then an extra 23%-25% savings in GWP could be attained there.  The pattern of feedstock efficiency is repeated when comparing the technologies on a scale basis. In fact, using more energy dense feedstock, i.e., feedstock that has a higher methane potential, leads to higher GWP savings for the small-scale facility (S + technology scenario), on a per ton feedstock basis, than for the industrial scale (I + technology scenario) (Figure 9). This is true even though the feedstock mix used in the small scale is more burdensome in terms of GWP, due to the cultivation phase of the feedstocks. The industrial scale facility still incurs savings to GWP, albeit lower, due to the poor characteristics of the feedstock utilized, which in this case is ca. 80% cow manure. Technology preference largely stays the same for both scales, though it is worth mentioning that a friendlier feedstock mix, i.e., with less first generation feedstocks, such as the one in the industrial scale is more important for the AD + PHB option, as can be observed when comparing S_AD + PHB and I_AD + PHB, which have savings of −15 and −25 kg CO 2 eq/ton, respectively. The pattern of feedstock efficiency is repeated when comparing the technologies on a scale basis. In fact, using more energy dense feedstock, i.e., feedstock that has a higher methane potential, leads to higher GWP savings for the small-scale facility (S + technology scenario), on a per ton feedstock basis, than for the industrial scale (I + technology scenario) (Figure 9). This is true even though the feedstock mix used in the small scale is more burdensome in terms of GWP, due to the cultivation phase of the feedstocks. The industrial scale facility still incurs savings to GWP, albeit lower, due to the poor characteristics of the feedstock utilized, which in this case is ca. 80% cow manure. Technology preference largely stays the same for both scales, though it is worth mentioning that a friendlier feedstock mix, i.e., with less first generation feedstocks, such as the one in the industrial scale is more important for the AD + PHB option, as can be observed when comparing S_AD + PHB and I_AD + PHB, which have savings of −15 and −25 kg CO2 eq/ton, respectively.

Sensitivity
Two parameters were tested to assess the sensitivity of the results: transport distance of the feedstock and the effect of a theoretical future green energy mix in the system.
The effect of transport varies depending on how well the technologies perform. The initial results, which include a 1 km transport distance were varied and transport was added up to 100 km. The result can be observed in Figure 10, where it is evident that a further transport distance can be allowed for the AD + Booster technology in both regions since this is the best performing technology. The point at which each technology scenario goes from GWP saving to GWP burden can also be seen in the graph. This point (the y-intercept) is 86, 99, and 42 kilometers respectively for BAV_AD, BAV_AD + Booster and BAV_AD + PHB, in Bavaria. In Veneto these distances are lower, because of the lower performance of the technologies in this region, where a transport distance below 59, 65, and 41 kilometers for VEN_AD, VEN_AD + Booster, and VEN_AD + PHB respectively, would ensure that the technologies continue to induce GWP savings. Needless to say, the lower the transport distances for the feedstock, the better the technologies perform.

Sensitivity
Two parameters were tested to assess the sensitivity of the results: transport distance of the feedstock and the effect of a theoretical future green energy mix in the system.
The effect of transport varies depending on how well the technologies perform. The initial results, which include a 1 km transport distance were varied and transport was added up to 100 km. The result can be observed in Figure 10, where it is evident that a further transport distance can be allowed for the AD + Booster technology in both regions since this is the best performing technology. The point at which each technology scenario goes from GWP saving to GWP burden can also be seen in the graph. This point (the y-intercept) is 86, 99, and 42 kilometers respectively for BAV_AD, BAV_AD + Booster and BAV_AD + PHB, in Bavaria. In Veneto these distances are lower, because of the lower performance of the technologies in this region, where a transport distance below 59, 65, and 41 kilometers for VEN_AD, VEN_AD + Booster, and VEN_AD + PHB respectively, would ensure that the technologies continue to induce GWP savings. Needless to say, the lower the transport distances for the feedstock, the better the technologies perform. The effect of switching the current production mix for the provisioning of process electricity and electricity offset with a future energy mix mainly composed of renewable sources is substantial for GWP results. For the regional assessment in Bavaria, all technology options result in impact burdens for GWP, while they continue to be impact savings for Veneto ( Figure 11). This is due to the feedstock mix emissions in Bavaria, which are no longer counterbalanced by high emissions savings from offsetting of electricity. As has been shown before [26,84], offsets from replacing GHG intensive sources of electricity production such as coal, diminish as 'green' energy sources are implemented in the energy grid. The implications of this are very important for technologies producing renewable fuels, as their potential to produce savings will be bound to this future component. The effect of switching the current production mix for the provisioning of process electricity and electricity offset with a future energy mix mainly composed of renewable sources is substantial for GWP results. For the regional assessment in Bavaria, all technology options result in impact burdens for GWP, while they continue to be impact savings for Veneto ( Figure 11). This is due to the feedstock mix emissions in Bavaria, which are no longer counterbalanced by high emissions savings from offsetting of electricity. As has been shown before [26,84], offsets from replacing GHG intensive sources of electricity production such as coal, diminish as 'green' energy sources are implemented in the energy grid. The implications of this are very important for technologies producing renewable fuels, as their potential to produce savings will be bound to this future component. On the other hand, BAV_AD + Booster, which is the worst performing scenario in terms of GWP continues to be the best performing scenario for most other impact categories in the green energy future SA (normalized midpoint results in Appendix B, Figures A4 and A5). As this clearly points to burden shifting the results were subjected to two single indicator interpretation methods to clarify the results. On the other hand, BAV_AD + Booster, which is the worst performing scenario in terms of GWP continues to be the best performing scenario for most other impact categories in the green energy future SA (normalized midpoint results in Appendix B, Figures A4 and A5). As this clearly points to burden shifting the results were subjected to two single indicator interpretation methods to clarify the results.

Single Score Interpretations
Single score results, via TOPSIS developed by applying the ArgCW-LCA methodology [75], with environmental weights relating the results to a European's consumption patterns, and an economic weight derived from the TEA are discussed in this section. When assessed through TOPSIS (Figure 12), the initial regional results are very clear. Technology preference does not change within each region no matter which weighting is given to the results. AD + Booster is always the preferred choice, whether there are equal weights and high or low weight is given to economics. Furthermore, when impacts are monetized ($) so that the costs of environmental protection are visualized, these results also agree with the ArgCW-LCA and equal weights (EW) TOPSIS results. From the figure it is clear that the AD + Booster is also the best performer in terms of economic preference in Veneto (going up to 90% econ level), and on the contrary AD + PHB appears to be the worst. However, it is worth noting that in the Veneto region, if environmental concerns are weighed more heavily (<55% econ level), it is not easy to single out one of the technologies as unequivocally the best performing option, since the results perform close to equally well. This is not the case for Bavaria where the more burdensome feedstocks result in a more indisputable preference for the AD + Booster option, which produces the most energy. The implication of these results, namely that the more burdensome the energy production is, the more important the energy offsets become, is even more obvious for the plant level assessment. Here we see that though the technology preference is always the same (AD + Booster > AD > AD + PHB), the relative difference between the options becomes smaller the higher the economic weight (approaching 90%) for the Industrial plant in Veneto. This is a different pattern than the one observed for the regional level, where the distance between options, with and without PHB, increases with economic weight, and as supported by the assessment of midpoint results, the technology scenarios are closer to each other when the feedstock mix contains more animal manures than crop residues (see Figure 9). The same trend is seen for the small-scale plant in Bavaria, where the distance between the AD + Booster and AD + PHB option decreases with increasing economic weight. Though in this case, the plant's economic performance, which is very low in comparison to the industrial plant, is an important factor pulling all technology options further from the ideal.
The green energy future sensitivity was also checked with the single indicator methodology. The results again showed to be robust in terms of technology preference for the assessment (Figures A6  and A7). It is important to point out, however, that if the decision was based solely on GWP, then when looking at the green energy future one would choose AD + PHB in Bavaria, but continue to choose the AD + Booster in Veneto ( Figure A6, Appendix B).
Overall the results are robust, though some clear patterns emerge. The single indicator results clearly highlight the dependency on the energy extraction efficiency of the options, which have increasing importance for regions with a more burdensome production, i.e., in the cultivation of energy crop for biogas production (the BAV and S scenarios). In this case, the electricity offsets are very important, not only for GWP, but all impact categories considered in an LCA, as evidenced by the single indicator preference. There are trade-offs when production utilizes a higher share of energy crops. On the one hand, electricity production is higher and with today's electricity mixes offsetting this type of production is highly valuable. On the other hand, it is worth noting that sustainability criteria for biofuels and biomass fuels might limit this type of production even more in the future. As it stands today, the renewable energy directive II sets out a cap on energy crops for renewable fuels and national caps are also present in various member states. The EC has also singled out feedstock of high potential for indirect land use change (iLUC), so that renewable fuels do provide the GHG reductions they are meant to bring. Though small plants are exempt from this cap (ca. <500 kW electric), one needs only to look at the German case, where around 50% of plants are small, as an example of how many small biogas plants can in fact have large consequences for how agricultural land is used.
The assessment also shows that varied production, i.e., not only energy, can be a viable option for plants with a high content of manures in the mix. In a future with an optimized PHB production this might be even more beneficial, also if we are to avoid the impacts of microplastic pollution, which are yet to be included in LCA studies. For now, strong subsidies are needed to increase technology penetration in the market with constant revision on sustainability targets. Continuing to green the energy grid should be a top priority by making as much energy as possible and fomenting technologies that increase the energy that can be obtained from biomass (like the AD booster). Future research on the possible synergies between technologies such as the AD-Booster + PHB could be interesting to explore. Booster > AD > AD + PHB), the relative difference between the options becomes smaller the higher the economic weight (approaching 90%) for the Industrial plant in Veneto. This is a different pattern than the one observed for the regional level, where the distance between options, with and without PHB, increases with economic weight, and as supported by the assessment of midpoint results, the technology scenarios are closer to each other when the feedstock mix contains more animal manures than crop residues (see Figure 9). The same trend is seen for the small-scale plant in Bavaria, where the distance between the AD + Booster and AD + PHB option decreases with increasing economic weight. Though in this case, the plant's economic performance, which is very low in comparison to the industrial plant, is an important factor pulling all technology options further from the ideal.

Conclusions
The production scale of the industrial set up assessed, with electricity ca. 1 MW and crude PHB production at ca. 300 ton/y, is small compared to their fossil and non-fossil alternatives. As a result, the financing, maintenance, and labor related costs increase the break-even prices significantly. Crude PHB production in AD plants requires the co-production of electricity in order to be adequately valorized, though benefits from avoided plastic particle pollution, which could be important, have not been included in the TEA and LCA. With today's energy mixes in the regions in question, it is highly valuable to offset electricity production and thereby options such as the AD + Booster are preferred for all environmental areas of protection. Material production in scenarios such as the AD + PHB perform equally well to more energy efficient scenarios for plants with a feedstock mix high in animal manures. Future caps on certain types of feedstock are worth considering when deciding on technology options to be implemented and/or subsidized.

Acknowledgments:
The authors would like to thank various members of the NoAW project for their unwavering support and crucial sharing of expert knowledge. In particular, we thank associated farms "La Torre" and "Schiessl" for providing data on their yearly operations. For their valuable insights on the technology scenarios, we thank Innoven and BioVantage, as well as prof. Majone. Finally, we thank Eng. Kayser for her help in finding data pertaining the status of biogas in Bavaria.