Opportunities for Improving the Environmental Profile of Silk Cocoon Production under Brazilian Conditions

Brazilian silk production is amongst the five largest in the world. Nonetheless, there is no life cycle assessment study on silk cocoon production and its upstream processes, pertaining to the mulberry production (fundamental upstream process for silk production), in the existing literature. The objective of this study was to identify opportunities to improve the environmental profile of mulberry and silk cocoon production under Brazilian conditions. To that end, a life cycle assessment was conducted for the core processes of mulberry and silk cocoon production and upstream processes of raw material production, using the ReCiPe method for life cycle impact assessment using nine impact categories. Overall, the mulberry production showed greater impacts than the cocoon production for the impact categories analyzed. A few opportunities for improving the environmental profile of mulberry and silk cocoon production under Brazilian conditions included replacing the Kraft paper used to cover the rearing beds, replacing the standard tractor used to fetch mulberry leaves, replacing light bulbs, conducting a more sustainable mulberry and cocoon production, and setting a reverse logistics system for plastic and paper packaging waste. Nevertheless, many of these measures are long-term strategies. Besides, many of them need further economic feasibility assessment.


Introduction
In the Western world, Brazil is the largest silk cocoon producer on a commercial scale [1]. Brazilian silk production is the fifth largest in the world. The world leaders in silk production are China at 146,000 t/y, India at 28,708 t/y, Uzbekistan at 1100 t/y, Thailand at 692 t/y, and Brazil at 560 t/y [2]. In the first half of 2019, Brazil exported around 108 million tons of textiles and apparel (excluding cotton fiber), and almost 689 million tons when including cotton fiber. Considering Brazilian exports of textile and apparel products, textile fibers and yarns total 622.603 million tons (cotton fiber included). Considering only natural textile fibers and yarns, the result is 616.745 million tons (cotton fiber included) [3,4].
Cotton is the most commonly recognized fiber, when considering natural fibers, as it is predominant in the context of textile production in Brazil and worldwide. However, silk has been gaining prominence all environmental impacts are conducted, aided by software tools and life cycle impact assessment methods. In (iv), all data and information from the previous phases are taken into consideration to enable informed decision-making, identifying where the main impacts of the system are (hotspots), and establishing a plan towards improving the system's environmental profile. It can be noted that those phases are iterative and changes in one might trigger changes in others. Moreover, phases (i), (ii), and (iii) are directly linked to interpretation (iv), and, as highlighted in the ISO 14040 [24], an LCA study shows potential impacts, thus those might not be absolute or precise. The interpretation needs to be conducted according to the intricacies of the system under study.
A couple of LCA studies on silk production could be found in the existing literature. Edlund et al. [26] addressed the environmental impacts of synthetic spider silk production from Escherichia coli, where the authors identified the global warming potential (GWP) in terms of greenhouse gases (GHGs) of the baseline E. coli process. Astudillo et al. [15] conducted an LCA of Indian silk, where the authors assessed the mulberry production, silkworm rearing, cocoon drying, and cocoon cooking-reeling. The authors identified the mulberry production to be the main environmental hotspot for silk production.
Nonetheless, to the best of our knowledge, to date, no studies have been identified on the life cycle assessment of Brazilian silk production. Based on the aforementioned, the research question that motivated this study was "how to optimize the environmental performance of mulberry and silk cocoon production under Brazilian conditions?" To that end, this study's aim was to identify opportunities to improve the environmental profile of mulberry and silk cocoon production under Brazilian conditions. This article is structured as follows. The first section provided the initial considerations on the need for investigating the environmental profile of silk production in Brazil and stated the objective of the article. Section 2 presents the methods used in this study. Section 3 depicts the results for the life cycle impact assessment of the mulberry and silk cocoon production. Section 4 presents opportunities for improving the environmental profile of silk cocoon production. Lastly, Section 5 draws on the conclusions, limitations, and suggestions for further and future research.

Methods
The LCA methodology, as described in the ISO standards for Life Cycle Assessment [24,27], and the LCA software Umberto NXT Universal were used to conduct this study. A cradle-to-gate (C2G) approach was used to define the boundaries of the system here addressed. The analysis was not restricted to the core processes (mulberry and cocoon production), including also all upstream processes (production of raw materials). Figure 1 illustrates the system boundaries and material flows for the system. Data gathering was mostly done in collaboration with actors of the supply chain.
The system is considered C2G since it embeds the production of raw materials (upstream processes), and mulberry and cocoon production (core processes), up to cocoon delivery at the gate of the silk yarn manufacturing company. Primary sources provided all input data for the core processes. Ecoinvent 3.3 life cycle inventory (LCI) datasets were used for modeling the upstream processes, hence, information about the production of raw materials was secondary. The detailed inventory can be seen in Appendix A (Tables A1 and A2).
The analyses were conducted with the ReCiPe Midpoint (H) [28], using a midpoint approach for nine impact categories. The ReCiPe method [29] is one of the most complete life cycle impact assessment methods in terms of a number of impact categories, and it received an update in 2016 [30]. Moreover, the method enables a harmonized implementation of cause-effect paths for calculating both midpoint and endpoint characterization factors, and its update provided characterization factors that are representative at a global scale, whilst still maintaining the possibility of impact categories to implement characterization factors at a country scale [31]. The impact categories analyzed in the analysis of this study were: Freshwater Ecotoxicity (FETP), Human Toxicity (HTP), Terrestrial Ecotoxicity (TETP), Climate Change (GWP), Freshwater Eutrophication (FEP), Agricultural Land Occupation (ALOP), Water Depletion (WDP), Terrestrial Acidification (TAP), and Natural Land Transformation (NLTP). The Ecoinvent v.3.3 database and the cut-off system were used for modeling the system [32]. Furthermore, the considered system does not generate any co-products up to the point of cocoon production. Therefore, allocation procedures were not necessary.
The reference flow for the present study was on a mass basis (1 kg of silk cocoon, including packaging). The functional unit for the study was 1 kg of silk cocoon (including packaging) delivered to the silk yarn manufacturing company.
Given that most of the processes could not be designed with information for Brazilian conditions, due to the lack of such in the LCI (Ecoinvent v.3.3) database [33], there is some uncertainty undergoing the presented system, since the reference used may not truly represent the local reality. The system is considered C2G since it embeds the production of raw materials (upstream processes), and mulberry and cocoon production (core processes), up to cocoon delivery at the gate of the silk yarn manufacturing company. Primary sources provided all input data for the core processes. Ecoinvent 3.3 life cycle inventory (LCI) datasets were used for modeling the upstream processes, hence, information about the production of raw materials was secondary. The detailed inventory can be seen in Appendix A (Tables A.1

and A.2).
The analyses were conducted with the ReCiPe Midpoint (H) [28], using a midpoint approach for nine impact categories. The ReCiPe method [29] is one of the most complete life cycle impact assessment methods in terms of a number of impact categories, and it received an update in 2016 [30]. Moreover, the method enables a harmonized implementation of cause-effect paths for calculating both midpoint and endpoint characterization factors, and its update provided characterization factors that are representative at a global scale, whilst still maintaining the possibility of impact categories to implement characterization factors at a country scale [31]. The impact categories analyzed in the analysis of this study were: Freshwater Ecotoxicity (FETP), Human Toxicity (HTP), Terrestrial Ecotoxicity (TETP), Climate Change (GWP), Freshwater Eutrophication (FEP), Agricultural Land Occupation (ALOP), Water Depletion (WDP), Terrestrial Acidification (TAP), and Natural Land Transformation (NLTP). The Ecoinvent v.3.3 database and the cut-off system were used for modeling the system [32]. Furthermore, the considered system does not

Data Quality
The data quality objective for this study was to use the data that most accurately represented the processes (production of the silk cocoon) under Brazilian conditions.

•
Reliability: Data for core processes (mulberry and cocoon production) were gathered together with the silk cocoon producers; • Completeness: Data from all processes were considered for a period of a year (2018-2019 production), evening out normal eventual fluctuations; • Temporal correlation: All data gathered were related to 2018-2019 production.

•
Geographical correlation: All inventory data for core processes (mulberry and cocoon production) were from the area under study, representing the local reality. The inventory data for upstream processes (production of raw material) were from Ecoinvent v.3.3; • Technological correlation: Data came from primary sources, directly acquired from the organizations involved in the study, via interviews, surveys, and meetings with internal and external stakeholders.

Assumptions and Limitations
As allocation method, for the upstream processes (production of raw materials) we used the cut-off, thus, all impacts were attributed to the process that generated it, not penalizing other entries [34]. Moreover, the geography Rest of the World (RoW) was given priority when modeling the processes that used secondary data, since inventories under Brazilian conditions are scarce. The choice was based on the fact that RoW is an exact copy of the Global geography, but with uncertainty adjustment [35]. Moreover, we used the process type Result for each process designed with secondary data, for it considers the aggregated environmental exchanges and the impacts of the product system related to a specific product of a specific activity [32].
Except for transportation, infrastructure processes (facilities, equipment necessary for production activities, both for mulberry and cocoon production) were not included within the system boundaries, thus their impacts were not accounted for.

Results
Overall, the mulberry production presented higher impacts than cocoon production, as it can be seen in Figure 2.
• Reliability: Data for core processes (mulberry and cocoon production) were gathered together with the silk cocoon producers; • Completeness: Data from all processes were considered for a period of a year (2018-2019 production), evening out normal eventual fluctuations; • Temporal correlation: All data gathered were related to 2018-2019 production. • Geographical correlation: All inventory data for core processes (mulberry and cocoon production) were from the area under study, representing the local reality. The inventory data for upstream processes (production of raw material) were from Ecoinvent v.3.3; • Technological correlation: Data came from primary sources, directly acquired from the organizations involved in the study, via interviews, surveys, and meetings with internal and external stakeholders.

Assumptions and Limitations
As allocation method, for the upstream processes (production of raw materials) we used the cutoff, thus, all impacts were attributed to the process that generated it, not penalizing other entries [34]. Moreover, the geography Rest of the World (RoW) was given priority when modeling the processes that used secondary data, since inventories under Brazilian conditions are scarce. The choice was based on the fact that RoW is an exact copy of the Global geography, but with uncertainty adjustment [35]. Moreover, we used the process type Result for each process designed with secondary data, for it considers the aggregated environmental exchanges and the impacts of the product system related to a specific product of a specific activity [32].
Except for transportation, infrastructure processes (facilities, equipment necessary for production activities, both for mulberry and cocoon production) were not included within the system boundaries, thus their impacts were not accounted for.

Results
Overall, the mulberry production presented higher impacts than cocoon production, as it can be seen in Figure 2. For most of the impact categories, mulberry production represented the greatest impacts, which is quantitatively shown in Table 1. For most of the impact categories, mulberry production represented the greatest impacts, which is quantitatively shown in Table 1.  Figure 3 shows the detailed analysis of impacts (by process) for mulberry production. The processes highlighted in bold blue font were the main contributors to environmental impacts.  Figure 3 shows the detailed analysis of impacts (by process) for mulberry production. The processes highlighted in bold blue font were the main contributors to environmental impacts. For Freshwater Ecotoxicity and Freshwater Eutrophication, the hotspots were in the processes of soil correction and maintenance, due to the use of phosphorus and the production of single superphosphate, which has emissions of metal to water, such as copper, nickel, manganese, and zinc.
For Human Toxicity, the hotspot was also in the soil maintenance, due to the single superphosphate production (emissions of manganese, arsenic, mercury, selenium, and lead), and the use of organic fertilizer, once again due to the emission of metals such as manganese, molybdenum, arsenium, and zinc to water and/or soil, and antimony, lead, and mercury to air.
For Natural Land Transformation, the greatest impact was due to the limestone residue remaining on the soil, which has a beneficial impact, in the process of soil correction. The negative impacts were due to the transformation of natural land due to poultry farming and the consequent production of organic fertilizer (poultry manure), and the production of single superphosphate for soil correction.
For Terrestrial Acidification, the hotspots were in the single superphosphate production, due to the emission of SOx and NOx, in the limestone production due to the emission of SOx, NOx, and ammonia, in the transport of organic fertilizer and the use of the tractor for the soil preparation, also due to the emission of SOx and NOx.
For the Terrestrial Ecotoxicity category, the hotspots were in the soil maintenance, due to the emissions of phosphorus to water and soil, in the transport of the organic fertilizer, due to the emission of metals (e.g., antimony, lead, and zinc to air, and zinc to soil), and in the production of organic fertilizer due to the emission of antimony, copper, and zinc to air, and zinc to soil. For Freshwater Ecotoxicity and Freshwater Eutrophication, the hotspots were in the processes of soil correction and maintenance, due to the use of phosphorus and the production of single superphosphate, which has emissions of metal to water, such as copper, nickel, manganese, and zinc.
For Human Toxicity, the hotspot was also in the soil maintenance, due to the single superphosphate production (emissions of manganese, arsenic, mercury, selenium, and lead), and the use of organic fertilizer, once again due to the emission of metals such as manganese, molybdenum, arsenium, and zinc to water and/or soil, and antimony, lead, and mercury to air.
For Natural Land Transformation, the greatest impact was due to the limestone residue remaining on the soil, which has a beneficial impact, in the process of soil correction. The negative impacts were due to the transformation of natural land due to poultry farming and the consequent production of organic fertilizer (poultry manure), and the production of single superphosphate for soil correction.
For Terrestrial Acidification, the hotspots were in the single superphosphate production, due to the emission of SO x and NO x , in the limestone production due to the emission of SO x , NO x , and ammonia, in the transport of organic fertilizer and the use of the tractor for the soil preparation, also due to the emission of SO x and NO x .
For the Terrestrial Ecotoxicity category, the hotspots were in the soil maintenance, due to the emissions of phosphorus to water and soil, in the transport of the organic fertilizer, due to the emission of metals (e.g., antimony, lead, and zinc to air, and zinc to soil), and in the production of organic fertilizer due to the emission of antimony, copper, and zinc to air, and zinc to soil.
For Water Depletion, the hotspots were in the soil maintenance and the mulberry cultivation, due to the consumption of water, and in the production of limestone and single superphosphate, for the amount of water used in their production.
While for the mulberry production the hotspots were identified in the impact categories Freshwater Ecotoxicity, Freshwater Eutrophication, Human Toxicity, Natural Land Transformation, Terrestrial Acidification, and Water Depletion. For the cocoon production, the hotspots were identified in the categories Agricultural Land Occupation, Climate Change, Human Toxicity, Natural Land Transformation, Terrestrial Acidification, Terrestrial Ecotoxicity, and Water Depletion. Figure 4 shows the detailed analysis of impacts (by process) for cocoon production. The processes highlighted in bold blue font were the main contributors to environmental impacts. For Agricultural Land Occupation, the hotspots were in the production of Kraft paper, used for covering the silkworms during the feeding/rearing process, and in the electricity consumption.
For Climate Change, the hotspots were in the transport of mulberry leaves to feed the silkworms, due to the emission of CO 2 and CH 4 to air, in the production of the Kraft paper used for covering the silkworms during the feeding/rearing process, once again due to emission of CO 2 and CH 4 , added to the emission of dinitrogen monoxide (N 2 O), and in the electricity production, due to the emission of CO 2 , N 2 O, and CH 4 .
For Human Toxicity, the greatest hotspot was in the barn disinfection, due to the use of chlorine, which emissions go to air, water, and soil, and the grid disinfection, due to the use of formaldehyde, which is largely emitted to air.
For Natural Land Transformation, hotspots could be seen in the use of electricity, due to the transformation of the natural land necessary for obtaining the Brazilian electricity mix; the transport of mulberry leaves to feed the silkworms, and the production of Kraft paper, used for covering the silkworms during feeding/rearing.
For Terrestrial Acidification, the greatest hotspot was given to the transport of mulberry leaves to feed the silkworms, due to the emission of NO x and SO x to air, and the production of Kraft paper, due to the emission of SO x , NO x , and ammonia to air.
For Terrestrial Ecotoxicity, the greatest hotspot is in the barn disinfection, due to the use of chlorine, and its emissions to air, water and soil, followed by the grid disinfection, due to the use of formaldehyde, and its emissions to air, and the Kraft paper production, due to the emission of cypermethrin (pesticide used in tree farming).
For Water Depletion, the greatest hotspot was in the silkworm feeding/rearing, and in the barn and grid disinfection, due to the consumption of ground well water.
Identifying the hotspots within the mulberry and cocoon production was extremely important for enabling finding potential improvements for the silk cocoon production under Brazilian conditions. The next section discusses a few implications of the impacts here identified and discusses a series of suggestions to improve the environmental profile of the silk cocoon production in Brazil.
The recommendations presented in the next section comprise the potential actions the producers are able to undertake. Further suggestions were considered by the authors. Nevertheless, the producers largely rely on the yarn manufacturing company for their subsistence, and the company provides them with guidelines for both the mulberry and cocoon production. From a practical perspective, producers will only take actions towards better environmental performance if such actions (i) comply with the yarn manufacturing company's policy, and (ii) it brings them economic gains. Kraft paper production (box) Chlorine (Barn disinfection) Lime production (barn disinfection) Grid disinfection Kraft paper production (bed cover) Transport (silkworm purchase) Lime production (bed sanitization) Polyethylene production (chlorine packaging) Polyethylene production (formaldehyde packaging) Liquefied petroleum gas (grid scorching) Kraft paper production (lime packaging) (barn disinfection) Polypropylene production (raffia sack) Transport (packed cocoons)

Discussions and Suggestions for Improving the Environmental Profile of Silk Cocoon Production in Brazil
From the results of the impact assessment, a series of suggestions aiming to improve the environmental profile of cocoon production were made. Initially, two suggestions were identified as easier to be implemented by the producers, which are presented in Scenario 1 and Scenario 2. The impacts presented in Section 3 refer to the existing practices for the presented system, thus referred to as "reference scenario".

Impact Reduction on the Cocoon Packaging
Traditionally, the cocoon producers (from the studied region) use raffia sacks to pack the cocoon for sale. Seeking a substitute for the raffia sacks, two scenarios were designed for further impact assessment. Scenario 1. Substitution of raffia sacks with jute sacks. Table 2 shows the impacts of the substitution of raffia sacks for jute sacks. A great increase of impacts in eight of the nine categories analyzed can be observed. Therefore, this option was discarded. Impacts only decreased for the category of Freshwater Ecotoxicity. Even though jute is a natural fiber, its production requires greater amounts of resources than for the raffia. Scenario 1 assessed only the packaging system, considering in the reference scenario the production of polypropylene, the manufacturing of the raffia sacks, and its incineration at the end-of-life phase, in comparison to the equivalent for jute production in Scenario 1.

Scenario 2.
Substitution of raffia sacks with cotton sacks. Table 3, in turn, shows the impacts of the substitution of the raffia sacks with cotton ones. Once again, impacts worsened. As observed in Scenario 1, even though cotton is also a natural fiber, its production requires greater amounts of resources than those of the raffia. Therefore, this option was also discarded.
An alternative strategy to reduce the impacts related to packaging, considering the packaging for the transport of the cocoon up to the delivery point, would be to ban the use of raffia sacks and instead use the plastic boxes that are already used by the yarn manufacturer to transport the cocoons from that cocoon delivery point to the yarn manufacturing site. Those boxes can be reused for a long period of time, and they could be distributed to the producers by the yarn manufacturing company since the boxes return to the company at every rearing cycle. This practice would potentially influence impact reduction on all the impact categories analyzed. Nonetheless, adopting such practice would need oversight and assessment in order to measure its effectiveness for impact reduction.

Scenarios 1 and 2. Replacing Incandescent Light Bulbs in the Barn with LED Ones.
The producers in the studied region (and likely in the country), use incandescent light bulbs in their facilities, mostly because they have lower prices. Using LED light bulbs can save up to 95% energy. Considering this reduction (95%) in the energy consumption for lighting, it can be noted that 83.61% of impacts derived from energy consumption can be avoided in all impact categories, as shown in Table 4. As the same percentage of energy consumption was applied to both Scenarios 1 and 2, the same impacts of energy consumption were avoided. The substitution of the type of light bulbs is a simple practice. However, the reduction of impacts has significant representativeness, reflecting directly in the impact categories of Climate Change, Agricultural Land Occupation, and Natural Land Transformation.
A series of other suggestions are made hereafter. However, these could not be simulated using scenarios, since the inventories that would be necessary to test them accordingly either (i) were not found available in the database the researchers had access to, or (ii) do not exist yet.

Reduction of the Impacts from the Use of Kraft Paper
The Kraft paper is commonly used by producers (in the studied region) to cover the silkworms on the rearing beds, in order to maintain a stable temperature. Nevertheless, due to the great impact generated from the Kraft paper production, an alternative for a possible reduction of this impact would be substituting the Kraft paper for another type of paper and/or material. The possibilities would be newsprint, nonwoven fabric (TNT), or even a lightweight fabric with good breathability, in the latter, case reuse would also be possible. In this case, it is necessary to follow up on the use of these alternate materials and assess future impacts. The substitution of the Kraft paper could result in a significant impact reduction for the categories of Agricultural Land Occupation, Climate Change, Natural Land Transformation, Terrestrial Acidification, and Terrestrial Ecotoxicity.

Reduction of the Impacts from the Use of a Standard Tractor
(a) Replacing the diesel-driven tractor with a biogas-driven one If the tractor used in the property were replaced with a biogas-driven one, the producer could use the silkworm litter from the rearing bed (which is currently just laid on the mulberry field, which practice has been reported to be of lesser efficiency compared to alternate handling (see Section 4.5, item b) to produce biogas and organic fertilizer (from the digestate) (see Salvador et al. [20]) that could be then still laid on the mulberry field. The biogas could be used to power the tractor, reducing/eliminating the need for diesel and consequently reducing its related impacts.
Furthermore, the economic feasibility of setting up a biodigester for such an end needs to be assessed. Nevertheless, as in the region where the study was conducted the properties are close to one another, it is worth exploring the possibility of arranging condominiums for energy recovery, where the producers could transport the rearing bed remains (silkworm litter + mulberry stems and uneaten leaves + lime residue) to a central and, from there, produce and distribute biogas and organic fertilizer.
(b) Replacing the standard tractor with a horse cart One of the main impacts was due to the transport of mulberry leaves to feed the silkworms, and it is currently done with a standard tractor. One of the options to try to lessen the environmental impacts of this process is to use a horse cart instead of the standard tractor. Other producers in the region make use of such practice. However, currently, this is largely due to financial constraints rather than the choice of being more environmentally friendly.

(c) Replacing the standard tractor with a micro tractor
Considering the impacts of the transport of mulberry leaves from the field to the barn, one can choose to use a different tractor, in this case, a micro tractor, whose use is indicated for several soil types, including sandy and clay soil, ideal for sites of small territorial extension. This tractor has a much lower fuel consumption than the conventional tractor [36,37]. However, further assessment is needed to make sure how much this change would reduce impacts.
The alternatives above mentioned can result in significant impact reduction for the categories of Terrestrial Acidification, Climate Change, Natural Land Transformation, and Terrestrial Acidification.

Reduction of the Impacts from Both Mulberry and Silk Cocoon Production by a More Sustainable Production
(a) Organic agriculture to reduce the impacts of chemical fertilizer and herbicide Chemical-based inputs are mostly used because of short term response and economic factors. However, chemical waste (from fertilizers, insecticides, herbicides, fungicides) can cause damage to silkworms, as mulberry leaves are the silkworm's only food. The promotion of biological or organic agriculture is necessary in order to reduce the impacts of mulberry production. Organic manure is another solution for reducing the impacts of chemical fertilizer use since physical, chemical, and biological properties of soil health are enhanced [38].
In this case, the use of biofertilizers replaces the use of pesticides in the cultivation of mulberry trees. The biofertilizer is composed of living microorganisms that assist the development, supply, and availability of primary nutrients to the plant [39].
(b) Optimizing the use of mulberry leaves waste during cutting, and the remains of the rearing beds (mulberry stems and dry leaves, and silkworm litter) On average, 30.35% is wasted during the harvest of mulberry leaves, this green mass could be better utilized by means of improved management and planning of sericulture activities. There are also the remains of the silkworm rearing bed, with a large volume of available organic matter that can best be harnessed through strategies and/or processes considering its nutrient richness [40].
Aerobic decomposition is an opportunity to optimize the use of this material, with advantages such as faster activity of organisms and absence of unpleasant odors, it does not generate slurry and does not lose nitrogen. Another intention with composting is to reduce the C:N ratio. The result of this process is a replacement for poultry manure (organic fertilizer with considerable impact on the mulberry production). Composting aids the production of healthy plants, decreases the need for chemical fertilizers while conserving natural resources and also brings economic and social benefits. In addition, it improves the chemical, physical and biological properties of the soil, promoting root development, with higher yield and quality in cultivation [39,41].
Another alternative is vermicomposting, which involves a collective activity of earthworms and microorganisms. In addition to being rich in nutrients, enzymes, antibiotics, plant growth hormones, and large beneficial microbial populations that enhance the quality and yield of mulberry leaves, which, in turn, increases silk productivity. The product resulting from vermicomposting is rich in all the nutrients the plant needs. The use of vermicomposting also enhances a 50% reduction in the use of chemical fertilizers [38,42].
The use of green fertilizer is also a practice that adds non-compound green plant tissue to the soil in order to improve its physical structure and fertility. Green fertilizer can replace organic manure, being distributed between the mulberry rows, as it provides "additional nitrogen because of its ability to fix nitrogen from the air with the help of its root nodule bacteria" [39] (pp. 7-8), on top of preventing the growth of weeds and, consequently, abolishing the cost of getting rid of them [39].
The mentioned practices can result in significant impact reduction for the categories of Freshwater Ecotoxicity, Freshwater Eutrophication, Human Toxicity, Natural Land Transformation, Terrestrial Acidification, Terrestrial Ecotoxicity, and Water Depletion.

Reduction of the Impacts from Packaging via Reverse Logistics
Reducing the divergent socioeconomic and environmental impacts caused by waste is critical, however, there are several factors involved that make this somewhat difficult, such as social, environmental, and economic issues, including geographical boundaries and links to other sectors [43].
The great quantity and variety of packages used, result in a large number of packages disposed of in landfills, causing those not to return to their production cycle. Returning packages to their steward allows for the manufacture of new products and prevents virgin raw material from being exploited, besides further reducing costs in terms of maintaining landfills and consequently extending resource value [44]. Thus, improved reverse logistics practices could be a way out to elucidate this issue [45].
The plastic packages used for chlorine and formaldehyde, and the paper packages used for lime, could be returned to their manufacturers to be given correct disposal. A reverse logistics system could be designed where these package wastes make their way back to their manufacturer or another party who would hold stewardship. The producers could store these packages and take them to the silk yarn manufacturer (who provides those inputs to them). The silk yarn manufacturer, in turn, could forward such materials to the respective provider, until these wastes get to their primary steward for adequate reuse/disposal. Implementation and management of packaging via a reverse logistics system can enable impact reduction on the impacts categories of Agricultural Land Occupation, Climate Change, Natural Land Transformation, Terrestrial Acidification, and Terrestrial Ecotoxicity.

Conclusions
From the large impact generated by the fiber and textile sector, there is a need to define strategies to reduce the impacts caused. Brazil is one of the largest silk producers in the world, and the demand for Brazilian silk is on the rise, therefore, attention is needed towards the impacts of Brazilian silk production. Hence, the objective of this study was to identify opportunities to improve the environmental profile of mulberry and silk cocoon production under Brazilian conditions. To that end, a life cycle assessment was conducted for the core processes of mulberry and silk cocoon production and upstream processes of raw material production.
Thus, it was possible to identify that the impacts of the mulberry production stood out in comparison to those of the silk cocoon production. Based on the identified impacts, several opportunities were identified towards improving the mulberry and silk production profile under Brazilian conditions. Opportunities for improvement include replacing incandescent light bulbs with LED ones, replacing Kraft paper used to cover the rearing bed, replacing the use of a standard tractor, measures for a more sustainable mulberry and cocoon production, reverse logistics of plastic and paper packaging. Although several opportunities to potentially reduce environmental impacts were identified, appropriate, tailored strategies need to be designed to overcome technical and socioeconomic barriers.
It is noteworthy that the silk yarn production needs to be included in the system boundaries to have a more complete view of the life cycle impacts of the silk production under Brazilian conditions. As of the time of this publication, the authors were trying to build a life cycle inventory of the next downstream phase, the silk yarn production. However, the contact with a company that can provide such information has not granted them this privilege thus far. Moreover, due to lack of collaboration, time, financial resources, and available inventories in the Ecoinvent database that allowed the authors to model the processes, they were unable to quantitatively test the recommendations made in Section 4.2 through Section 4.6.
For future studies, when possible, under Brazilian conditions, it is proposed to conduct a life cycle assessment study that includes the suggestions for improvement made here, in order to verify how much the reported impacts can be reduced. Moreover, research on improving the impacts of these processes not only on the environment, but also on society and the economy can be expected. Furthermore, it is also suggested to include the silk yarn production within the system boundaries for a more comprehensive picture of Brazilian silk production. Sustainability 2020, 11, x FOR PEER REVIEW production. Hence, the objective of this study was to identify opportunities to im environmental profile of mulberry and silk cocoon production under Brazilian condition end, a life cycle assessment was conducted for the core processes of mulberry and s production and upstream processes of raw material production.
Thus, it was possible to identify that the impacts of the mulberry production sto comparison to those of the silk cocoon production. Based on the identified impac opportunities were identified towards improving the mulberry and silk production pro Brazilian conditions. Opportunities for improvement include replacing incandescent light LED ones, replacing Kraft paper used to cover the rearing bed, replacing the use of a standa measures for a more sustainable mulberry and cocoon production, reverse logistics of p paper packaging. Although several opportunities to potentially reduce environmental im identified, appropriate, tailored strategies need to be designed to overcome tech socioeconomic barriers.
It is noteworthy that the silk yarn production needs to be included in the system bou have a more complete view of the life cycle impacts of the silk production under Brazilian c As of the time of this publication, the authors were trying to build a life cycle inventory downstream phase, the silk yarn production. However, the contact with a company that ca such information has not granted them this privilege thus far. Moreover, due to lack of col time, financial resources, and available inventories in the Ecoinvent database that allowed t to model the processes, they were unable to quantitatively test the recommendation Sections 4.2 through 4.6.
For future studies, when possible, under Brazilian conditions, it is proposed to con cycle assessment study that includes the suggestions for improvement made here, in orde how much the reported impacts can be reduced. Moreover, research on improving the these processes not only on the environment, but also on society and the economy can be Furthermore, it is also suggested to include the silk yarn production within the system b for a more comprehensive picture of Brazilian silk production.   Source: Based on [40]