Distribution Models of Timber Species for Forest Conservation and Restoration in the Andean-Amazonian Landscape, North of Peru

The Andean-Amazonian landscape has been universally recognized for its wide biodiversity, and is considered as global repository of ecosystem services. However, the severe loss of forest cover and rapid reduction of the timber species seriously threaten this ecosystem and biodiversity. In this study, we have modeled the distribution of the ten most exploited timber forest species in Amazonas (Peru) to identify priority areas for forest conservation and restoration. Statistical and cartographic protocols were applied with 4454 species records and 26 environmental variables using a Maximum Entropy model (MaxEnt). The result showed that the altitudinal variable was the main regulatory factor that significantly controls the distribution of the species. We found that nine species are distributed below 1000 m above sea level (a.s.l.), except Cedrela montana, which was distributed above 1500 m a.s.l., covering 40.68%. Eight of 10 species can coexist, and the species with the highest percentage of potential restoration area is Cedrela montana (14.57% from Amazonas). However, less than 1.33% of the Amazon has a potential distribution of some species and is protected under some category of conservation. Our study will contribute as a tool for the sustainable management of forests and will provide geographic information to complement forest restoration and conservation plans.


Introduction
The Amazon is the most extensive and widely distributed forest region (approximately 70 million hectares) and is subjected to permanent forest resource extraction in Peru [1]. The Peruvian Amazonia comprises 60% of the country and is marked by a large degree of floral diversity. The floral activities are unique in nature in the fragile and complex ecosystem of the Andean-Amazonian transitional landscape [2]. In most forests, selective logging focuses on harvesting only specific individual trees of high commercial value [3,4]. In this way, it is a direct cause of deforestation and indirectly promotes shifting agriculture, which it makes a hurdle towards sustainable forest management for the Peruvian Amazon [5].

Study Area
The Peruvian Amazon is located in the Andean-Amazonian landscape of northern Peru. This part of the Amazon is mainly characterized by premontane, montane cloud, and lowland forest [2], and is also marked by altitude, which represents a wide variety of fauna and flora diversity [40]. There are many emerging tree species in this region, such as Cedrelinga, Cedrela, Buchenaria, Dipteryx, Sloanea, and Podocarpus; underneath emerging trees lie species like Hevea, Hernandia, Calophyllum, Qualea, and Inga [41]. The region covers an area of approximately 39.2 thousand km 2 of rugged territory ( Figure 1). The study area is located between the parallels 3 • 0 15 and 7 • 2 0 south and the meridians 77 • 0 15 and 78 • 42 15 west, with an altitudinal gradient that ranges from 120 m above sea level (a.s.l.) in the north to 4400 m a.s.l. in the south. The region shares a border on the east side with the Loreto region, on the west side with the Cajamarca region, on the south side with the San Martín and La Libertad regions, and on the north side with the Ecuadorian territory [42]. Qualea, and Inga [41]. The region covers an area of approximately 39.2 thousand km² of rugged territory ( Figure 1). The study area is located between the parallels 3°0′15" and 7°2′0" south and the meridians 77°0′15" and 78°42′15" west, with an altitudinal gradient that ranges from 120 m above sea level (a.s.l.) in the north to 4400 m a.s.l. in the south. The region shares a border on the east side with the Loreto region, on the west side with the Cajamarca region, on the south side with the San Martín and La Libertad regions, and on the north side with the Ecuadorian territory [42].

Methodological Design
We adapted the methodologies proposed by OSINFOR [43] and Hengl et al. [44] and applied by Rojas et al. [45] to assess the biogeographical distribution of the ten forest timber species. The detailed methodological framework adopted by this study is shown in Figure 2. From the perspective of management practices and forest exploitation plans granted for the Amazonas region, we selected ten (10) species that have been highly exploited and unrooted over time [46]. The cartography for environmental variables was obtained and homogenized from the WorldClim web portal (http://worldclim.org/version2). The soil variables were obtained from SoilGrids ver. 0.5.3 (http://soilgrids.org), and the physiographic variables were obtained from the United States Geological Survey (USGS) (http://srtm.usgs.gov). The collected datasets were grouped according to their contribution to the modeling.
In this study, we adopted an SDM-based MaxEnt approach to represent the spatial distribution of the ten most exploited timber species in Amazonas, Peru. Finally, all potential distribution areas obtained were overlapped with degraded areas identified by the Ministry of the Environment (http://geoservidor.minam.gob.pe) for validation. This study was done in an effort to obtain the areas for restoration. Likewise, overlapping was done with natural areas protected by the Peruvian state (http://geo.sernanp.gob.pe) in order to establish the species distribution in conservation areas at the local, regional, and national scale.

Methodological Design
We adapted the methodologies proposed by OSINFOR [43] and Hengl et al. [44] and applied by Rojas et al. [45] to assess the biogeographical distribution of the ten forest timber species. The detailed methodological framework adopted by this study is shown in Figure 2. From the perspective of management practices and forest exploitation plans granted for the Amazonas region, we selected ten (10) species that have been highly exploited and unrooted over time [46]. The cartography for environmental variables was obtained and homogenized from the WorldClim web portal (http://worldclim.org/version2). The soil variables were obtained from SoilGrids ver. 0.5.3 (http://soilgrids.org), and the physiographic variables were obtained from the United States Geological Survey (USGS) (http://srtm.usgs.gov). The collected datasets were grouped according to their contribution to the modeling.

Selection and Collection of Occurrences of the Most Important Forest Species in the Amazonas Region
The ten most exploited timber species in the region were identified, taking as a starting point the entry into force of Law No. 29763 (LFFS, October 1, 2015) until 2019. Data on forest species occurrence were collected using a global positioning system (GPS) to provide the locations of timber trees prior to their use. However, species grown in plantations and/or gardens were not considered [47][48][49]. There was a total of 4454 recorded units of data for the 10 species of interest, which were subsequently systematized to meet the MaxEnt requirements. The ten timber species with the greatest demand during 2016-2019 were given volume in round cubic meters (Vol. m 3 (r)) ( Table 1; Supplementary  Table S1). Of these 10 species, five were evaluated by Rojas et al. [45] as being the most exploited in 2018. However, in this study, in addition to the restoration approach, they were analyzed under a system of conservation and coexistence among a greater number of species. In addition, the species under study are in different statuses of conservation (Table 1) in the IUCN (International Union for Conservation of Nature) Red List of Threatened Species (https://www.iucnredlist.org/).  In this study, we adopted an SDM-based MaxEnt approach to represent the spatial distribution of the ten most exploited timber species in Amazonas, Peru. Finally, all potential distribution areas obtained were overlapped with degraded areas identified by the Ministry of the Environment (http://geoservidor.minam.gob.pe) for validation. This study was done in an effort to obtain the areas for restoration. Likewise, overlapping was done with natural areas protected by the Peruvian state (http://geo.sernanp.gob.pe) in order to establish the species distribution in conservation areas at the local, regional, and national scale.

Selection and Collection of Occurrences of the Most Important Forest Species in the Amazonas Region
The ten most exploited timber species in the region were identified, taking as a starting point the entry into force of Law No. 29763 (LFFS, October 1, 2015) until 2019. Data on forest species occurrence were collected using a global positioning system (GPS) to provide the locations of timber trees prior to their use. However, species grown in plantations and/or gardens were not considered [47][48][49]. There was a total of 4454 recorded units of data for the 10 species of interest, which were subsequently systematized to meet the MaxEnt requirements. The ten timber species with the greatest demand during 2016-2019 were given volume in round cubic meters (Vol. m 3 (r)) ( Table 1; Supplementary  Table S1). Of these 10 species, five were evaluated by Rojas et al. [45] as being the most exploited in 2018. However, in this study, in addition to the restoration approach, they were analyzed under a system of conservation and coexistence among a greater number of species. In addition, the species under study are in different statuses of conservation (Table 1) in the IUCN (International Union for Conservation of Nature) Red List of Threatened Species (https://www.iucnredlist.org/).

Basic and Thematic Cartography Conditioning
Cartographic information on political-administrative boundaries within the district, province, and region was obtained from the National Geographic Institute's (IGN) Fundamental Geospatial Data Infrastructure (https://www.idep.gob.pe). The georeferenced records of the ten species and the cartographic information were projected to a system of geographic coordinates in WGS 84 using Open Source QGIS version 3.8 Zanzibar (https://qgis.org/es/site/).

Bioclimatic, Soil, and Physiographic Variables
The spatial distribution of species within the landscape is based on differential selection of environmental factors that interact and favor their optimal development [50]. In this context, 26 environmental variables were selected, including 19 bioclimatic variables, 3 topographic variables, 3 soil property variables, and solar radiation information ( Table 2). The bioclimatic information and solar radiation layers were obtained from the WorldClim (http://worldclim.org/version2) portal. This version of WorldClim provides a spatially interpolated monthly climate data set for global land areas, with a spatial resolution of 1 km. These variables were aggregated over a time range from 1970 to 2000 (30 years) and from 9000 weather stations and 60,000 satellite coverages [51]. The topographic variables were derived from the 90 m spatial resolution Shuttle Radar Topography Mission (SRTM) Digital Elevation Model (DEM), which was obtained from the United States Geological Survey (USGS) web portal (http://srtm.usgs.gov). The spatial information of physical and chemical soil properties was obtained from SoilGrids version 0.5.3 (http://soilgrids.org) with a resolution of 250 m. This information was generated from 150,000 soil profiles and from 158 covariates obtained from remote sensing using a set of automatic learning methods: random forest with gradient boosting and/or multinomial logistic regression [44].
The cartographic conditioning of the environmental variables and the species records were worked at a standardized spatial resolution of 250 m. The management of the post-modeling spatial information was carried out in the open source GIS software QGIS ver. 3.10. Mean Diurnal Range (monthly mean (max temp-min temp)) Temperature Seasonality (standard deviation × 100) Mean Temperature of Wettest Quarter Mean Temperature of Coldest Quarter Annual Precipitation mm Bio13 Precipitation

Extraction of Values of Climatic, Soil, and Physiographic Variables
SDMs are widely used to predict the distribution of a species across geographic space and time using environmental data. However, this has generated new concerns regarding inaccuracies and lack of understanding. To address these issues, MaxEnt takes advantage of the existing collinearity to find the best set of parameters. Collinearity between environmental variables can cause certain problems in terms of model over-adjustment, increased uncertainty, and decreased statistical significance [52,53]. Therefore, for each species, variables to be excluded from the initial model were determined in order to have a second and final application of the MaxEnt model. In this way, the "Analysis of variable contributions" table was constructed with the 26 inaugural variables and was supported by the results of a "Jackknife" analysis. This analysis allowed the model to be run by excluding each of the variables at a certain time. The variable that considerably affected the model's efficiency at the time of being excluded from the model was considered important [14].

Variable Correlation and Clustering
Pearson's correlation coefficients (r) between the 19 bioclimatic variables, 3 topographic variables, 3 soil variables, and solar radiation were calculated in the R software (version 3.6) for multi-collinearity tests. The variables whose correlation coefficients were greater than 0.8 were eliminated not only to avoid the violation of statistical assumptions, but also to avoid model predictions induced by multi-collinearity between variables [54][55][56][57]. First, values of Euclidean distances were introduced from value extraction through the k-means classification tool using the factoextra package. This determined the optimal number of variable groups for each forest species, dividing the data sets into meaningful classes [58]. Dendrograms were then constructed to group climatic, topographic, and soil variables that shared similar characteristics, whereas those with different characteristics were separated into another group. This was done prior to MaxEnt modeling, which used the variables with the greatest contributions to the final model.

Model Execution
The potential distribution model for each species were generated by a machine learning algorithm based on the maximum entropy principle [14] using the open-source software MaxEnt ver. 3.4.1 (https://biodiversityinformatics.amnh.org/open_source/maxent/). This algorithm estimates the probability of potential distribution of each species, taking into account that the best prediction is obtained by maximizing the entropy of that distribution under certain environmental contexts [14]. The maximum entropy principle was first expounded by E. T. Jaynes, who also discussed its theoretical and mathematical background [59].
For model validation, random data corresponding to the presence records of each species were selected, where 75% for training and 25% for model validation were considered, respectively [14]. The algorithm was run using 10 replicates in 5000 iterations with different random partitions (cross-validation method), a convergence threshold of 0.00001, and 10,000 maximum background points. Other settings (e.g., extrapolation, drawing graphs, etc.) were kept as default [21], since MaxEnt is able to select the appropriate function for the number of samples used for a model [25,60]. Subsequently, the model output was validated based on the area under the curve (AUC) [14,61] calculated from the receiver operating characteristic (ROC) [62]. According to the AUC values, five performance levels are differentiated [14,61,63]: excellent (>0.9), good (0.8-0.9), accepted (0.7-0.8), bad (0.6-0.7), and non-valid (<0.6). The main advantage of this method is, in fact, its threshold independence and the objectivity of the results [64]. Therefore, we used the logistic output format to obtain the current models of the ten species evaluated [65]. This format allowed the generation of a map of continuous probability values for the distribution in a range from 0 to 1. Furthermore, these values were reclassified into four ranges [66]: (1) "high" potential habitat (>0.6), (2) "moderate" habitat (0.4-0.6), (3) "low" habitat (0.2-0.4), and (4) "non-potential" habitat (<0.2). In addition, to assess coexistence, we intersected the high potential distribution among the ten species. The result was reclassified into high (>6 species), moderate (4-6), and low (1-3) coexistence. Similarly, degraded areas were intersected with the coexisting distribution of species, identifying high (>6 species), moderate (4-6), and low (1-3) levels, with potential to be restored with the species under study.

Identification of Potential Areas for Conservation and Restoration
In this stage, the layers of potential forest species areas were superimposed on the map of degraded areas of Peru [67]. This map identified four degradation classes grouped into three degradation categories: "low" (forest fragments), "medium" (negative Net Primary Productivity (NPP) and forest fragments, negative NPP, or changes in vegetation cover), and "high" (deforestation 2001-2017). This analysis made it possible to obtain the potential areas for restoration using forest species throughout the study area. Likewise, the layer of potential areas was superimposed on Amazonas's natural protected areas in their different categories (natural protected areas, reserve areas, private conservation areas) (http://geo.sernanp.gob.pe/geoserver/principal.php), and the conservation concessions granted by the Executive Directorate of Forest and Wildlife Management (DEGBFS) and the Regional Conservation Areas (RCAs) administered by the Amazonas Regional Government, as well as the cartographic information available in the Amazonas Regional Government's Spatial Data Infrastructure (IDER) platform (http://geoportal.regionamazonas.gob.pe), were also included.

Biographical Distribution of Timber Species
The MaxEnt model was applied to the 10 timber species with a cumulative contribution of >65% of only three environmental variables, highlighting the contributions for the modeling of the species C. montana (98.9%), C. decandra (93.8%), and C. spruceanum (90%), which were mainly driven by the topographic variable "elevation" (Table 3). Likewise, the Jackknife test obtained for each model showed that the variables elevation (DEM) and Bio 18 (Precipitation of Warmest Quarter) contributed independently in the largest number of models of the species (Supplementary Figures S11-S20).

Model Performance
As a result, there were ten biogeographic distribution models, each corresponding to the timber species under study. Indeed, the average yield of nine out of the 10 species showed AUC > 0.9, reflecting excellent predictive performance of the models [19,57,59], except for C. montana, which was rated as good (0.8 < AUC < 0.9). The mean AUC values reported for the 10 species ranged from 0.868 (the lowest) to 0.985 (the highest) ( Table 4). Under current edaphoclimatic conditions, the general climatic suitability (sum of "high", "moderate", and "low" potential habitat) for the ten timber species under study was mainly distributed in the northern part of the Amazonas region, which includes the Bagua and Condorcanqui provinces. On the other hand, we found that the C. montana species were located in the central and southern parts of Amazonas. Figure 3b-k shows the biogeographical distribution areas of ten timber species. In the same distribution context, the species C. cateniformis and O. parvifolia present a greater distribution high in the northern part of the study area.  Table 5 describes the surface area (km 2 ) of each species by level of potential distribution and the percentage that this surface represents with respect to the total study area (42,050.37 km 2 ). C. montana has the largest total potential distribution range with 17,107.10 km 2 , covering 40.68% of the Amazon territory, followed by C. cateniformis with 8619.21 km 2 (20.50%) and O. parvifolia with 7730.28 km 2 (18.38%). For their part, C. spruceanum and C. odorata cover only 2.33% (977.80 km 2 ) and 3.63% (1527.93 km 2 ), respectively. A total of 14.97% (6299.79 km 2 ) of the Amazon has conditions to host species in  Table 5 describes the surface area (km 2 ) of each species by level of potential distribution and the percentage that this surface represents with respect to the total study area (42,050.37 km 2 ). C. montana has the largest total potential distribution range with 17,107.10 km 2 , covering 40.68% of the Amazon territory, followed by C. cateniformis with 8619.21 km 2 (20.50%) and O. parvifolia with 7730.28 km 2 (18.38%). For their part, C. spruceanum and C. odorata cover only 2.33% (977.80 km 2 ) and 3.63% (1527.93 km 2 ), respectively. A total of 14.97% (6299.79 km 2 ) of the Amazon has conditions to host species in coexistence, with a high potential distribution of 3.73 km 2 that could support more than six species coexisting together.

Potential Areas of Restoration and Coexistence of Species
From the degraded areas identified by the Ministry of the Environment of Peru ( Figure 4a) and their intersection with the potential biographical distribution of the ten timber species (Figure 3b−k), potential restoration areas were identified with each one of these species (Figure 4b−k). Likewise, the levels of coexistence of the species are presented (Figure 4l). In Table 6, three levels of restoration can be distinguished according to each level of potential distribution. In other words, the higher the potential distribution level, the greater the potential for restoration with this species. C. montana (1221.79 km 2 ), O. parvifolia (491.96 km 2 ), and C. cateniformis (449.20 km 2 ), are the species that cover a larger area with high restoration potential. A total of 6.56% (2758.45 km 2 ) of Amazonas can be restored with the ten species under study, of which six or more species can be installed in a combined way to restore 1.35 km 2 of degraded territory in Amazonas.

Potential Biogeographic Distribution in Conservation Areas
From the high potential distribution of the ten timber species (Figure 3b-k) and their intersection with the Regional Conservation System (Figure 5a), the presence of one or more species within a conservation category was identified (Figure 5b, Table 7). From the highest to lowest efficiency in conserving forests with the evaluated species, the categories are: Conservation Concessions-CC (288.34 km 2 ), Private Conservation Area-PCA (222.06 km 2 ), Reserve Zone (RZ) (209.34 km 2 ), Regional Conservation Area-RCA (140.14 km 2 ), and Protected Natural Area-PNA (46.70 km 2 ). The species A. leiocarpa is the one that is more widely distributed in CC (23.30 km 2 ), C. spruceanum

Discussion
This study shows the potential biogeographic distribution of ten endangered timber species over the Peruvian Amazonia along with the spatial distribution of degraded areas for restoration with natural forest species through novel MaxEnt modeling techniques using vast amounts of the numerical and remote sensing data. Starting from the revision of the forest management plans, it was possible to locate 4454 georeferenced trees, considering scientific species names and the wood volume [68], which corresponded to ten timber species that were approved through a system of concessions,

Discussion
This study shows the potential biogeographic distribution of ten endangered timber species over the Peruvian Amazonia along with the spatial distribution of degraded areas for restoration with natural forest species through novel MaxEnt modeling techniques using vast amounts of the numerical and remote sensing data. Starting from the revision of the forest management plans, it was possible to locate 4454 georeferenced trees, considering scientific species names and the wood volume [68], which corresponded to ten timber species that were approved through a system of concessions, permits, and authorizations [69], fundamental tools that guarantee the sustainability of forests [70]. In this regard, based on the review of management plans, it was possible to locate 4454 georeferenced trees that corresponded to the ten timber species. Although their distribution is concentrated in the northern part of Amazonas and below 1000 m, we found an exception with C. montana (Figure 3e), whose distribution is above 1500 m, which is well documented by the botanical collections reported for these species [71][72][73]. This is because elevation has a significant impact on local climate, which influences the vegetation's growth and character [74].
The spatio-temporal distribution represents a major basis for understanding a species and its past, present, and future in order to establish forest management strategies [75]. Our study was based on a Maximum Entropy model, which outperforms other forms of SDM in predictive accuracy and tolerance to sample size when the sample is small [60]. In fact, it uses only present data for modeling [76]. Using bioclimatic (19), topographic (3), edaphic (3), and solar radiation variables [44,51,77], the biogeographic distribution of ten timber species was determined, covering 19.45% of the total surface of Amazonas. However, it is possible to find species that have the ability to grow outside their natural range, often with better development than in their native zone due to assisted migration programs and climate change [78]. It is, therefore, possible to find trees or plantations outside the potential range of the species presented in this research.
has not yet been widely studied [86], including species such as I. marginata, I. bourgonnii, I. tocacheana, I. tessmannii, I. multinervis, I. acreana, I ruiziana, and I. punctate, which are widely distributed in the northern part of the Peruvian Amazonas region [86].
Once the distribution of a species is known, it is important to design and complement protection networks to safeguard biodiversity, which is a difficult task for governments and conservationists in megadiverse countries [92]. As a matter of fact, the least protected species are usually the most threatened [93], as is the case of Cedrela odorata L., which is categorized as a vulnerable species (VU) [94]. Therefore, future conservation policies must be considered, taking into account goods and services and promoting a sustainable environment [95]. C. montana is in the endangered category (EN), C. cateniformis and C. pentandra are in the least concern (LC) category, and the other species are not included in the IUCN Red List. Protected areas represent the cornerstone of conservation strategies for protecting biodiversity through in situ approaches [96]. Based on what has been described, the Amazonas region has 18.03% of its territory under conservation areas (Figure 5a) with biological diversity. This allows for research in slightly altered ecosystems [97], and also strengthens the Regional System of Regional Conservation (SICRE), whose objective is to contribute to the development of the region through the effective management of conservation areas, articulation of national, regional, and private conservation areas, and concessions for conservation [98].
A total of 278,249 hectares of territory have been identified in the region of Amazonas as having high to very high priority for restoration, such as areas suffering from deforestation, soil erosion, forest fires, and illegal logging. However, no plan has been implemented yet [99]. Therefore, it is important to develop restoration actions, as they are a great opportunity to reduce poverty, control the effects of climate change, and protect the environment [100]. Hence, there is an urgent need to practice forest restoration to maintain natural ecosystems [101], which is an important natural solution for mitigating climate change [102]. There is increasing evidence that mixed-species forests can provide multiple ecosystem services [103], combining tree species that can provide multiple ecosystem services at higher levels [104,105]. The coexistence of the ten timber species in Amazonas (Figure 4l) allows for the recovery of degraded forests resulting from overharvesting, fires, grazing, or other disturbances or land uses [106] through the establishment of enrichment plantations, natural regeneration management, and agroforestry systems [107], through the combined installation of native wood species with fast (Simarouba amara), medium (Dypterix ferrea), and slow (Swietenia macrophylla) growth, and with nitrogen-fixing species, such as Inga edulis (guaba). The species S. macrophylla "mahogany", C. odorata "cedar", Amburana cearensis "ishpingo", and C. cateniformis "screw" are more competitive with other species during their growth, and they are implemented through silvicultural practices for the regeneration of secondary and degraded forests [108].
Finally, this research provides a basis for future studies on potential areas for other relevant species in order to monitor in situ systems, including the identification of areas with high or low reforestation potential, and also to develop forest management strategies [109]. It is also time to encourage efforts to restore and regenerate degraded areas in the region of Amazonas. For this purpose, it is important that reforestation should be a complementary rather than competitive livelihood activity, thus fostering local social equity and analyzing and complementing information that will enable restoration objectives to be achieved [110].
The biogeographic modeling for the 10 forest species obtained an average AUC of 0.95, which is considered an excellent yield. The species with the greatest range of potential distribution were C. montana (40.68% of the territory), C. Cateniformis (20.50%), and O. parvifolia (18.38%). On the other hand, C. spruceanum and C. odorata cover only 2.33% and 3.63%, respectively. The main limitation of distribution was due to the altitude variable, with the study area presenting an altitudinal range of 120 m a.s.l. in the north up to 4400 m a.s.l. in the southern part. Therefore, the greatest biogeographic distribution of the studied species is concentrated in the north of the department of Amazonas and below 1000 m a.s.l., although we find an exception with C. montana, whose distribution is above 1500 m a.s.l.
The high biogeographic distribution of one or more species is protected under some category of conservation, and C. montana and C. decandra are the species with the greatest potential distribution ranges in these areas. In addition, it was identified that eight of the ten species can coexist, and the species with the highest percentage of potential restoration area is C. montana (14.57% of Amazonas). This study was carried out in the Peruvian Amazon; however, it is applicable in other ecosystems, such as tropical and temperate forests, allowing the generation of tools for the sustainable management of forests and promoting the success of forest restoration and conservation.

Conflicts of Interest:
The authors declare no conflict of interest.