E ﬀ ect of Bactericides on Control of Acidiﬁcation Pollution and Spontaneous Combustion of Coal Gangue Dumps in China and Its Mechanism

: Oxidation of pyrite in the coal gangue dumps usually results in acidiﬁcation and spontaneous combustion, causing many environmental problems such as air, soil, and water pollution. The oxidizing bacteria exacerbate problems such as acidiﬁcation, spontaneous combustion, and explosions. The bacterium Acidithiobacillus ferrooxidans was ﬁrst separated and isolated from coal gangue samples. Bactericides such as Triclosan, Kathon (isothiazolinones), and sodium dodecyl sulfate (SDS) were selected for our study. Our ﬁndings indicated that the addition of bactericide e ﬀ ectively inhibited the oxidation of Fe 2 + , preventing pH decreases and oxidation-reduction potential increases. We also investigated the bactericidal mechanisms employed by the three bactericides against A. ferrooxidans by conducting a protein ﬂocculation test, scanning electron microscopy, and time-of-ﬂight mass spectrometry. We found that the speciﬁc inhibitory activities of the three bactericides di ﬀ ered. Kathon treatment caused A. ferrooxidans to release small amounts of proteins and lipids. A. ferrooxidans treated with Triclosan released small amounts of lipids and large amounts of plasmas. SDS caused the bacteria to release a large amount of proteins and lipids and degraded the surface structure of the cells, resulting in altered cell morphology.


Introduction
Coal gangue is an inevitable product of the process of coal mining and processing and is the main pollution source in coal mines [1]. The main source of acid coal gangue pollution and subsequent environmental disasters is the oxidation of sulfide. In the open storage process, sulfide contained in coal gangue easily forms acid mine drainage (AMD) containing high concentrations of sulfate and a diversity of harmful heavy metal ions through comprehensive actions such as air oxidation, rainwater eluviation, and microorganism activity [2,3]. Therefore, controlling the oxidation of sulfides in coal gangue has become an important means of mitigating the effects of acidification of coal gangue.
Studies in China and abroad have found that the oxidation process of pyrite in coal gangue is affected by the biocatalysis of oxidizing bacteria such as Acidithiobacillus ferrooxidans (A. ferrooxidans), significantly accelerating the conversion of Fe 2+ to Fe 3+ , increasing the reaction rate by 50 to 60 times, and playing a vital role in acid pollution [4]. In 1947, Colmer et al. discovered and isolated a strain of

Test Strains and Drugs
The original bacterial strain used in this study came from a coal gangue dump in Yangquan, Shanxi, China. A coal gangue sample (5 g) was added to 50 mL of sterile water and oscillated at 160 rpm for 3 days at a constant temperature of 30 • C. The solution was then filtered through paper to obtain a coal gangue soak solution containing bacteria; 70 mL of iron-free 9K medium (pH = 1.80, sterilized at 121 • C, 30 min), 20 mL of coal gangue soak solution, and 10 mL of Fe 2+ stock solution (filter-sterilized; Sustainability 2020, 12, 6697 3 of 10 containing 40 g/L Fe 2+ in the form of FeSO 4 ·7H 2 O) were combined. The flask was incubated in a thermostatic bath (30 • C, 160 rpm). After 7 days, the solution became reddish brown, iron precipitates formed, and the bacterial numbers reached 10 7 -10 8 /mL. A. ferrooxidans was sub-cultured twice in the liquid medium and after serial dilution plated on to a solid medium (0.3% sodium citrate and 0.016% CuSO 4 in 9K, 2% agarose; all in w/v). Single colonies were selected for RNA extraction (FastDNA spin kit for soil, MP Biomedicals, Irvine, CA, USA) and 16S rRNA gene sequencing (ABI 3730XL, Applied Biosystems, Foster City, CA, USA). The strain was identified to be a 99.04% homologous to Acidithiobacillus ferrooxidans strain YTW (DQ062116.1) by using 16S rRNA gene sequencing and named Acidithiobacillus ferrooxidans strain AF-14 (MT774373).

Determination of Optimal Application Concentrations
Bacterial solution (10 mL), 70 mL of iron-free 9K medium (heat sterilized as described above) and 10 mL of the Fe 2+ stock solution (also described above) were added to fifteen 250 mL sterilized conical flasks at an aseptic operation station. Kathon (10 mL) was added from sterile, 10-fold concentrated stock solutions to final concentrations 0 (control), 15, 30, 64 and 122 mg/L in the cultures. Each bactericide concentration was tested in triplicate. Similar procedure was followed with Triclosan and SDS, Triclosan with final concentrations of 0, 4, 8, 16, 28, and 36 mg/L (each tested in duplicate), SDS with final concentrations of 0, 5, 10, 30, 50, and 100 mg/L (each tested in duplicate). The control group was supplemented with 100 mL of 9K (Fe 2+ ) medium, sealed, and cultured at a constant temperature and rate of agitation (30 • C, 160 rpm). To determine the optimal application concentration of Kathon, the pH of the bacterial solution, the oxidation-reduction potential Eh, and the rate of Fe 2+ oxidation were measured at different times. The pH and Eh were measured by pH and redox electrode (coupled to pH and Eh meters, DZS-708L, INESA Scientific Instrument Co., Ltd. Shanghai, China). Ferrous iron concentrations were determined by the EDTA complexometric titration [20].

Disk Inhibition Zone Experiment
The disk inhibition zone experiment is an experimental method to determine the bactericidal effect by the degree of spread of bactericides on solid culture medium; 0.1 mL of bacterial culture was evenly spread onto each of 12 plates containing the solid medium described in Section 2.1. Triplicate plates were labeled No. 1-No. 4 and treated with 0.02 mL of Kathon (30 mg/L), Triclosan (16 mg/L), SDS (10 mg/L) and sterile water (control treatment), respectively. A 6 mm diameter hole was punched in the center of solid culture medium in each petri dish, and bactericide or sterile water was injected into the holes. After that, the petri dishes were placed in an incubator at a constant temperature of 30 • C for about 15 d, and the size of disk inhibition zone was observed.

Protein Flocculation Test
Five replications of the bacterial solution (cultures grown in 9K without the addition of any bactericide) were centrifuged at 10,000 rpm for 20 min, and the cells were collected. The cells were mixed well with 1 mL EDTA, transferred to a 2 mL EP tube, and centrifuged at 12,000 rpm for 10 min. The supernatant was discarded. This was repeated one more time, to remove metal ions from the medium.
The 1 mL cell suspensions in EDTA were used as positive controls (No. 1), with 980 µL sodium phosphate buffer (SPB) and 122 µL of MT buffer from a total DNA extraction kit (FastDNA spin kit for Sustainability 2020, 12, 6697 4 of 10 soil, MP Biomedicals) added. After mixing well, the solution was lysed in Matrix E tubes. When the solution temperature reached room temperature, it was shaken in a fastprep (bead mill oscillator) for 40 s. This was repeated six times before the cell beads were crushed.
As a negative control, 1 mL of full 9K medium (containing Fe 2+ ) was added to No. 5, mixed, and let stand for 3 h.
All samples, No. 1-No. 5, were centrifuged at 12,000 rpm for 10 min, and the supernatant was transferred to new 2 mL EP tubes. After, the protein precipitation solution (PPS, 250 µL) was added, and the tubes were inverted. The role of the PPS solution was to denature the protein. Treatment with PPS produced a milky flocculent material. Photographs were taken and observations made with a stereomicroscope. The appearance of a milky precipitate indicated a release of proteins as a result of bactericide treatment.

Observations Using a Scanning Electron Microscope
The bacterial solution (300 mL, in Section 2.1) to be tested was divided into 12 centrifuge tubes (25 mL). Triplicate plates were labeled No. [1][2][3][4]. Each group had 4 centrifuge tubes, centrifuged at 13,000 rpm for 20 min. The supernatants were discarded, and the precipitates containing bacteria were collected. The precipitates were then suspended in 1 mL of acidified H 2 O (with HCl to pH 1.8), transferred to 1.5 mL centrifuge tubes, centrifuged for 10 min at 12,000 rpm. The supernatant was discarded, leaving the bacteria bearing precipitate (iron removal). No. 1 was suspended in 1 mL of Kathon, No. 2 with 1 mL of Triclosan, No. 3 with 1 mL of SDS, and No. 4 with 1 mL of sterile water. These were cultured for 3 h on a shaking table at 160 rpm with a constant temperature of 30 • C. They were centrifuged at 12,000 rpm for 10 min. The supernatants were removed and combined with 1 mL of phosphate buffer (0.047 mol/L g of Na 2 HPO 4 ·12H 2 O and 0.020 mol/L of KH 2 PO 4 , with the pH adjusted to 7.2 with H 2 SO 4 ) and repeated 2 more times. The sample was fixed with 2.5% glutaraldehyde, held overnight at 4 • C, and centrifuged at 9000 rpm for 15 min, after which the supernatants were discarded and the samples were dehydrated with a gradient of 30%, 50%, 75%, 90%, and 100% ethanol. The cells were then freeze-dried at different gradient temperatures of −20 • C, −40 • C, −60 • C, and −80 • C, allowed to stand at each temperature step for 12 h, then observed using a scanning electron microscope (SEM).

Time-of-Flight Mass Spectrometry for the Determination of Intracellular Lipid Efflux
Bacterial solutions (10 mL) were mixed with iron-free 9K medium (70 mL, described above), Fe 2+ stock solution (10 mL, described above), and sterile water (10 mL) in 250 mL sterilized conical flasks, which were sealed and cultured under constant temperature and agitation (30 • C, 160 rpm).
Four sample groups (10 mL) were centrifuged at 1000 rpm for 5 min. The supernatant was transferred to a new centrifuge tube and centrifuged at 10,000 rpm for 10 min. The supernatants were discarded, and the viscous material attached to the walls of the centrifuge tubes was washed with 1 mL of Kathon, Triclosan, SDS, or sterile water at a pH of 1.8. These were then cultured at 30 • C and 160 rpm for 3 h and subjected to time-of-flight mass spectrometry. Figure 1 shows the differences in the inhibition rate at different bactericide concentrations. All the Fe 2+ in the control treatment was oxidized rapidly within 48 h. Following the addition of bactericide, the rate of Fe 2+ oxidation decreased to some extent in each treatment, probably because the growth of AF-14 was inhibited.

Effect of Different Concentrations of Bactericides on Fe 2+ in Culture Medium
Sustainability 2020, 12, 6697 5 of 10 addition of the biocide isothiazolinone up to 100 mg/L kills bacteria and reduces the release of Cu, Zn, Fe, and sulphate by 5% to 50% for different types of mine waste [13]. As seen in Figure 1a, as the Kathon concentration reached a critical value at 30 mg/L, the Fe 2+ oxidation rate in the bacterial solution increased slightly within the first 48 h and subsequently reached a stable value of 24.35-25.75%. The pH and oxidation-reduction potential (Eh) can also reflect the oxidation of Fe 2+ . As seen in Figure 1b, when the concentration of Kathon was below 30 mg/L, the pH of bacterial solution first increased (within 0-36 h) and then decreased. The reason is that a large amount of acid was consumed during Fe 2+ oxidation, which increased the pH of the bacterial solution significantly. Subsequently, greater amounts of Fe 3+ were oxidized, and the jarosite formed after hydrolysis caused the pH of the bacterial solution to drop rapidly. In addition, the time course of Eh ( Figure 1c) followed the course of Fe 2+ oxidation in Figure 1a. In Kathon treatments with concentration >30 mg/L, no changes in pH or Eh were observed, indicating that 30 mg/L of the bactericide effectively inhibits the oxidation of Fe 2+ .
Similarly, concentration of Triclosan >16 mg/L significantly reduced the oxidation rate of Fe 2+ in the culture to 14.62 to 16.52%. Higher Triclosan concentrations (>16 mg/L) did not reduce Fe 2+ oxidation rate further, indicating 16 mg/L was the optimal application concentration. There was no significant difference between the treatments with different concentrations of Triclosan, indicating that the optimal application concentration of Triclosan was 16 mg/L. The optimal application concentration for SDS was 10 mg/L, and the oxidation rate of Fe 2+ could be inhibited to 16.24% or less after 5 days. Repeated 100 mg/L of SDS treatment performed by Schippers et al. resulted in long term inhibition of leaching microorganisms in 65 m 3 percolators [11]. Our experimental results were much lower than this value, and they were consistent with the study by Onysko et al., which indicated that ≥5 mg of SDS per liter effectively inhibited biological ferrous iron oxidation [9].

Comparison of the Inhibition Effect of Bactericides on AF-14 Growth
AF-14 grew brownish-red colonies on solid media, and a disk inhibition zone without colonies was formed on the medium in contact with the bactericide because AF-14 growth was inhibited. As  Figure 1 shows that there is a critical value that constitutes an optimal application concentration. When the concentration is less than this value, increasing the bactericide concentration significantly reduces the Fe 2+ oxidation. When the concentration reaches this value, increasing the bactericide concentration does not have an obvious effect on Fe 2+ oxidation. Sand et al. demonstrated that an addition of the biocide isothiazolinone up to 100 mg/L kills bacteria and reduces the release of Cu, Zn, Fe, and sulphate by 5% to 50% for different types of mine waste [13]. As seen in Figure 1a, as the Kathon concentration reached a critical value at 30 mg/L, the Fe 2+ oxidation rate in the bacterial solution increased slightly within the first 48 h and subsequently reached a stable value of 24.35-25.75%.
The pH and oxidation-reduction potential (Eh) can also reflect the oxidation of Fe 2+ . As seen in Figure 1b, when the concentration of Kathon was below 30 mg/L, the pH of bacterial solution first increased (within 0-36 h) and then decreased. The reason is that a large amount of acid was consumed during Fe 2+ oxidation, which increased the pH of the bacterial solution significantly. Subsequently, greater amounts of Fe 3+ were oxidized, and the jarosite formed after hydrolysis caused the pH of the bacterial solution to drop rapidly. In addition, the time course of Eh ( Figure 1c) followed the course of Fe 2+ oxidation in Figure 1a. In Kathon treatments with concentration >30 mg/L, no changes in pH or Eh were observed, indicating that 30 mg/L of the bactericide effectively inhibits the oxidation of Fe 2+ .
Similarly, concentration of Triclosan >16 mg/L significantly reduced the oxidation rate of Fe 2+ in the culture to 14.62 to 16.52%. Higher Triclosan concentrations (>16 mg/L) did not reduce Fe 2+ oxidation rate further, indicating 16 mg/L was the optimal application concentration. There was no significant difference between the treatments with different concentrations of Triclosan, indicating that the optimal application concentration of Triclosan was 16 mg/L. The optimal application concentration for SDS was 10 mg/L, and the oxidation rate of Fe 2+ could be inhibited to 16.24% or less after 5 days. Repeated 100 mg/L of SDS treatment performed by Schippers et al. resulted in long term inhibition of leaching microorganisms in 65 m 3 percolators [11]. Our experimental results were much lower than this value, and they were consistent with the study by Onysko et al., which indicated that ≥5 mg of SDS per liter effectively inhibited biological ferrous iron oxidation [9].

Comparison of the Inhibition Effect of Bactericides on AF-14 Growth
AF-14 grew brownish-red colonies on solid media, and a disk inhibition zone without colonies was formed on the medium in contact with the bactericide because AF-14 growth was inhibited. As shown in Table 1, the diameter of disk inhibition zone differed for different treatments. The injection of sterile water into the holes had no effect on growth of AF-14, and the diameter of the disk inhibition zone was equal to the hole diameter. The diameters of disk inhibition zones of the three bactericides were SDS > Triclosan > Kathon from greatest to smallest, indicating SDS had the greatest inhibitory effect on growth of AF-14. The bactericidal strength of bactericide was proportional to the diameter of disk inhibition zone, so that SDS had the greatest inhibitory effect on growth of AF-14.

Effects of Bactericides on Bacterial Proteins
In Figure 2, No. 1 is a positive control sample, in which the bacteria were disrupted using a bead mill oscillator. The resulting complete release of proteins from the cells formed a milky flocculation precipitate. On the other hand, no flocculation precipitates were observed in a negative control (No. 5; a culture without any disruptions or bactericide additions). No. 2 was a bacterial solution treated with Kathon. The flocculation precipitate formed in this sample was greater than in No. 3 treated with Triclosan that showed no flocculation precipitate. The comparison indicates that Kathon causes protein efflux from AF-14 cells, while Triclosan does not. The protein flocculation precipitate in No. 4 with SDS was slightly greater than that in No. 2, suggesting that SDS had an even stronger effect on protein efflux than Kathon.
Sustainability 2020, 12, x FOR PEER REVIEW 6 of 10 shown in Table 1, the diameter of disk inhibition zone differed for different treatments. The injection of sterile water into the holes had no effect on growth of AF-14, and the diameter of the disk inhibition zone was equal to the hole diameter. The diameters of disk inhibition zones of the three bactericides were SDS > Triclosan > Kathon from greatest to smallest, indicating SDS had the greatest inhibitory effect on growth of AF-14. The bactericidal strength of bactericide was proportional to the diameter of disk inhibition zone, so that SDS had the greatest inhibitory effect on growth of AF-14.

Effects of Bactericides on Bacterial Proteins
In Figure

Effects of Bactericides on Bacterial Surfaces
As seen in Figure 3a, AF-14 was full in shape, smooth on surface, and had good light refraction. As shown in Figure 3b, 3 h after SDS was added, the cells were damaged and shrunken. The cell shape was distorted, with the presence of a viscous material on the surface. Consistent with Siebert et al., these findings demonstrated that SDS causes cell lysis, releasing nucleic acids [21]. This indicated that SDS destroyed the bacteria surfaces, inhibiting A. ferrooxidans activities.
As seen in Figure 3c, after 3 h of treatment with Kathon, AF-14 established with obvious cracks, shrinkage on cell surfaces, and large depressed areas and damage. In Figure 3d, Triclosan treatment The above results suggest that a critical amount of Kathon had the same effect as SDS. However, Triclosan alone had a negligible effect on the proteins of AF-14.

Effects of Bactericides on Bacterial Surfaces
As seen in Figure 3a, AF-14 was full in shape, smooth on surface, and had good light refraction.  Scanning electron microscopy demonstrated that although SDS damaged the bacterial cell surface of AF-14, the cell contents were not released. After treatment with Kathon or Triclosan, irreversible damage to AF-14 had occurred. These results demonstrated that all three bactericides effectively inhibited AF-14, and that the inactivation occurred at different points of action, to different extents. These results may explain the finding of the study by Sand et al. that SDS only transiently reduced the number and activity of bioleaching bacteria, but isothiazolinone reduced the release of metals and sulphate to some extent [13].  As shown in Figure 3b, 3 h after SDS was added, the cells were damaged and shrunken. The cell shape was distorted, with the presence of a viscous material on the surface. Consistent with Siebert et al., these findings demonstrated that SDS causes cell lysis, releasing nucleic acids [21]. This indicated that SDS destroyed the bacteria surfaces, inhibiting A. ferrooxidans activities.

Effects of Bactericides on Bacterial Lipids
As seen in Figure 3c, after 3 h of treatment with Kathon, AF-14 established with obvious cracks, shrinkage on cell surfaces, and large depressed areas and damage. In Figure 3d, Triclosan treatment changed the cellular morphology, and caused the release of a large amount of plasma; further, the surfaces of the cells adhered to the mucus and were corroded.
Scanning electron microscopy demonstrated that although SDS damaged the bacterial cell surface of AF-14, the cell contents were not released. After treatment with Kathon or Triclosan, irreversible damage to AF-14 had occurred. These results demonstrated that all three bactericides effectively inhibited AF-14, and that the inactivation occurred at different points of action, to different extents. These results may explain the finding of the study by Sand et al. that SDS only transiently reduced the number and activity of bioleaching bacteria, but isothiazolinone reduced the release of metals and sulphate to some extent [13]. Figure 4a shows the time-of-flight mass spectrometry results for the blank control group; Figure 4b-d represent the spectra for bacterial solutions treated with bactericides SDS, Triclosan, and Kathon, respectively.

Effects of Bactericides on Bacterial Lipids
In Figure 4a there are no peaks between 600 Da and 900 Da, whereas there were such peaks observed in Figure 4b-d. After comparison, a marker at 676.3 Da was detected, and the corresponding lipid structure was obtained by referring to the database Lipidbank, as shown in Figure 5.
There was almost no lipid efflux in the blank control (Figure 4a). When treated with SDS, the cell lipids were released at high volumes (Figure 4b). The time-of-flight mass spectrometry for the solution treated with Triclosan (Figure 4c) also showed a peak between 600 Da and 900 Da, which indicated that some lipids could be secreted and that the internal structure of the bacteria was compromised.
In contrast, the peak value between 600 Da and 900 Da for the Kathon treatment was lower and indicated a milder effect on lipids (Figure 4d).
These results show that the three bactericides had an effect on the lipids of AF-14, causing them to exit the cells to different extents. Among these treatments, SDS had a greater effect on lipids than did Triclosan and Kathon.
surface of AF-14, the cell contents were not released. After treatment with Kathon or Triclosan, irreversible damage to AF-14 had occurred. These results demonstrated that all three bactericides effectively inhibited AF-14, and that the inactivation occurred at different points of action, to different extents. These results may explain the finding of the study by Sand et al. that SDS only transiently reduced the number and activity of bioleaching bacteria, but isothiazolinone reduced the release of metals and sulphate to some extent [13].  In Figure 4a there are no peaks between 600 Da and 900 Da, whereas there were such peaks observed in Figure 4b-d. After comparison, a marker at 676.3 Da was detected, and the corresponding lipid structure was obtained by referring to the database Lipidbank, as shown in Figure 5.

Effects of Bactericides on Bacterial Lipids
There was almost no lipid efflux in the blank control (Figure 4a). When treated with SDS, the cell lipids were released at high volumes ( Figure 4b). The time-of-flight mass spectrometry for the solution treated with Triclosan (Figure 4c) also showed a peak between 600 Da and 900 Da, which indicated that some lipids could be secreted and that the internal structure of the bacteria was compromised. In contrast, the peak value between 600 Da and 900 Da for the Kathon treatment was lower and indicated a milder effect on lipids (Figure 4d).
These results show that the three bactericides had an effect on the lipids of AF-14, causing them to exit the cells to different extents. Among these treatments, SDS had a greater effect on lipids than did Triclosan and Kathon.

Conclusions
(1) Microbially mediated oxidation of pyrite in coal gangue dumps is the key process resulting in acidification and spontaneous combustion. The bactericides such as Triclosan, Kathon, and SDS can effectively inhibit the oxidation of iron by A. ferrooxidans, with the inhibition of Fe 2+ oxidation   In Figure 4a there are no peaks between 600 Da and 900 Da, whereas there were such peaks observed in Figure 4b-d. After comparison, a marker at 676.3 Da was detected, and the corresponding lipid structure was obtained by referring to the database Lipidbank, as shown in Figure 5.
There was almost no lipid efflux in the blank control ( Figure 4a). When treated with SDS, the cell lipids were released at high volumes ( Figure 4b). The time-of-flight mass spectrometry for the solution treated with Triclosan (Figure 4c) also showed a peak between 600 Da and 900 Da, which indicated that some lipids could be secreted and that the internal structure of the bacteria was compromised. In contrast, the peak value between 600 Da and 900 Da for the Kathon treatment was lower and indicated a milder effect on lipids (Figure 4d).
These results show that the three bactericides had an effect on the lipids of AF-14, causing them to exit the cells to different extents. Among these treatments, SDS had a greater effect on lipids than did Triclosan and Kathon.

Conclusions
(1) Microbially mediated oxidation of pyrite in coal gangue dumps is the key process resulting in acidification and spontaneous combustion. The bactericides such as Triclosan, Kathon, and SDS can effectively inhibit the oxidation of iron by A. ferrooxidans, with the inhibition of Fe 2+ oxidation reaching 75-83.5%. The optimal application concentrations of Kathon, Triclosan, and SDS for A. ferrooxidans inhibition were 30 mg/L, 16 mg/L, and 10 mg/L, respectively.
(2) All three bactericides effectively inhibited A. ferrooxidans and inactivated the cells to various extents, using different mechanisms. Among these, Kathon caused A. ferrooxidans to release small amounts of proteins and lipids, resulting from cracks and shrinkage to cell surfaces after 3 h of treatment. Triclosan caused the release of a small amount of lipid, due to cell surface shrinkage and degradation after 3 h. This caused the release of a large amount of plasma. Within 3 h, SDS caused a large release of proteins and lipids, degraded the cell surface structure, and distorted the cellular morphology of the bacteria.
(3) The main source of acid coal gangue dump pollution and consequent environmental disasters has been the oxidation of sulfides in coal gangue. Catalytic oxidation by microorganisms (A. ferrooxidans) has been identified as a major mechanism; therefore, the use of bactericides that can

Conclusions
(1) Microbially mediated oxidation of pyrite in coal gangue dumps is the key process resulting in acidification and spontaneous combustion. The bactericides such as Triclosan, Kathon, and SDS can effectively inhibit the oxidation of iron by A. ferrooxidans, with the inhibition of Fe 2+ oxidation reaching 75-83.5%. The optimal application concentrations of Kathon, Triclosan, and SDS for A. ferrooxidans inhibition were 30 mg/L, 16 mg/L, and 10 mg/L, respectively.
(2) All three bactericides effectively inhibited A. ferrooxidans and inactivated the cells to various extents, using different mechanisms. Among these, Kathon caused A. ferrooxidans to release small amounts of proteins and lipids, resulting from cracks and shrinkage to cell surfaces after 3 h of treatment. Triclosan caused the release of a small amount of lipid, due to cell surface shrinkage and degradation after 3 h. This caused the release of a large amount of plasma. Within 3 h, SDS caused a large release of proteins and lipids, degraded the cell surface structure, and distorted the cellular morphology of the bacteria.
(3) The main source of acid coal gangue dump pollution and consequent environmental disasters has been the oxidation of sulfides in coal gangue. Catalytic oxidation by microorganisms (A. ferrooxidans) has been identified as a major mechanism; therefore, the use of bactericides that can effectively kill A. ferrooxidans and inhibit oxidation can help reduce the acid water and the risk of spontaneous combustion in coal gangue by fixing sulfur and lowering temperatures. Together, these strategies can control the spread of pollution and promote vegetative restoration.