Composted Chicken Manure for Anaerobic Soil Disinfestation Increased the Strawberry Yield and Shifted the Soil Microbial Communities

: Anaerobic soil disinfestation (ASD), as a bio-fumigation technology, has been developed to control soil-borne pests. There is increasing evidence showing that carbon sources and cover tarps play an important role in the ASD suppression of soil-borne pests, but little is known about the e ﬀ ect of composted chicken manure (CCM) and totally impermeable ﬁlms (TIF) against soil-borne pests in the strawberry production system. In experiments, the colonies of Fusarium spp. and Phytophthora spp., which are recognized to cause strawberry soil-borne diseases, decreased signiﬁcantly after ASD. The soil promoted a signiﬁcant increase in ammonium nitrogen, nitrate-nitrogen and organic matter, but a decrease in oxidation-reduction potential after ASD. Besides, the strawberry plant height, stem thickness and yield were signiﬁcantly higher than in the non-amended soil. Compared to the untreated control, ASD, both at 6 and 12 ton / ha of CCM, signiﬁcantly ( p = 0.05) increased strawberry marketable yield and income. The economic beneﬁt could be due to the suppression of soil-borne diseases and the improvement of soil nutrition. The soil bacterial and fungal diversity and richness increased after soil fumigation. The increased presence of biological control agents led to the suppression of soil-borne pathogens. In summary, ASD with CCM amendments could be applied in pre-plant fumigation to control strawberry soil-borne pests, strengthen soil fertility, improve crop yield and increase growers’ income.


Introduction
Soil-borne diseases are caused by fungi, bacteria, weeds and underground pests, and lead to severe crop losses [1,2]. Nonetheless, pre-plant soil fumigation technology is the main method for tackling strawberry soil-borne pests worldwide [3]. However, the chemical fumigants cause environmental problems and personal safety risks [4,5]. For example, chloropicrin (Cip) and 1,3-Dichloropropene (1,3-D) are the major fumigants for strawberry plants, and are facing increased regulatory pressure around the world. [6,7]. Strawberry (Fragaria × ananassa) is a major high-economic crop, and plays an important role in increasing farmers' incomes [8]. Considering the long-term sustainability of

Field Trials
Field trials were carried out in Trial I (Jingjiao, 40 • 36 30.30 E) during the 2017-2018 season, Beijing, respectively. The greenhouses used for the field trials had continuously grown strawberry plants for many years, and faced high soil-borne disease pressures caused by Fusarium spp. and Phytophthora spp. (Table 1). All the greenhouses had never fumigated with chemical fumigation before the experiments. The soil was sandy loam (Trial I: 60% sand, 37% silt and 3% clay; OM = 23.6 g kg −1 ; pH 7.9. Trial II: 57% sand, 37% silt and 6% clay; OM = 13.0 g kg −1 ; pH 7.5, respectively.). The CCM (23% C, 3.8% P and 3.4% K; pH 8.4) was from the Hebei Fengshou Green Organic Fertilizer Co., Ltd., and the 0.05-mm thickness TIF was provided by Shouguang Longxing Plastic Film Technology Co., Ltd., Shandong Province, China.  Three commercial greenhouses (two greenhouses in Trial I and one greenhouse in Trial II) were used for the field experiments. Before the tests started, the soil was ploughed twice with a 25-horsepower greenhouse rotary cultivator. According to the experimental design, the greenhouses Sustainability 2020, 12, 6313 3 of 15 were divided into 12 plots at each trial site. Each treatment was set up with 4 replicates in each trial site, and all plots were arranged in a completely randomized design. The areas of each plot of Trial I and Trial II were 22 and 28 m 2 , respectively. The treatments included: (1) 6 tons/ha composted chicken manure (CCM 6); (2) 12 tons/ha composted chicken manure (CCM 12); and (3) blank control (CK), no amendment. A TIF mulch was used to cover the soil in each plot. CCM 6 and CCM 12 were spread evenly on the soil surface. A rotary tiller was used to plough the plot again and the drip irrigation pipes were placed uniformly at 70-cm distances. According to our trial design, TIF was buried around each plot to prevent air exchange and water leakage. Finally, a drip irrigation system was used, and 50 tons/ha of water was applied. Each treatment was set up with 4 replicates in each trial site, and all plots were arranged in a completely randomized design. After covering the plot with TIF for 21 days, the film was opened and removed from each plot to allow air exchange for 7 days. The experiments were carried out on 27 June 2017 and 12 July 2017 for Trial I and Trial II, respectively. The strawberries (cultivar "Red Face") were transplanted on 28 August 2017 and 30 September 2017, in Trial I and Trial II, respectively.

Soil Sampling
The five soil samples method was used to take soil samples from the soil (0-20 cm depth) in each plot at the sampling time. Soil samples were collected at 0 days (pre-fumigation), 30 days (post-fumigation, 26 July 2017 for Trial I and 10 August 2017 for Trial II) and 120 days (post-fumigation, 23 October 2017 for Trial I and 8 November 2017 for Trial II) from the 2 trial sites. After that, each soil sample was sieved through a 2-mm sieve, and sent in an icebox to the laboratory for analysis. Soil samples, collected in triplicates after 30 day-treatment, were analyzed in order to study soil-borne fungi and physical-chemical index. (a) 200 g of fresh soil was stored at 4°C to isolate fungi pathogens. (b) 150 g of dry soil was passed through a 40-mm sieve, and stored at room temperature for soil physical and chemical analysis, and (c) 40 g fresh soil samples were collected in Trial II and stored at -80°C for DNA extraction.

Fungal Soil-Borne Pathogen Analysis
The continuous cultivation of strawberries on the same land over many years has increased the risk of soil-borne diseases caused by Fusarium and Phytophthora [22,23]. The colonies of Fusarium spp. and Phytophthora spp. were isolated from 15 g fresh soil samples. Fusarium spp. and Phytophthora spp. were quantified using the methods described by Komada [24] and Masago [25], respectively.
The ingredients of selective media for the cultivation of Fusarium spp. and Phytophthora spp. were reported in (Table 2). Fungi colonies were cultured at 28 • C for 3 days, and the quantity (colony-forming units (CFU) per g −1 soil) of fungi oomycetes was counted for data analysis. * Take the amount of 2 L as an example. The ingredients in A were added to 1.9 L distilled water, boiled, packed and then sterilized. The ingredients in B were directly added to 100 mL of sterilized water and shaken.

Soil Physical and Chemical Properties
Potassium concentration was detected by a model AP 1500 flame photometer (Shanghai Instruments Group Co., Ltd., Shanghai, China) [26]. Soil pH and oxidation-reduction potential were measured in a soil suspension (1:2.5 soil to H 2 O ratio) by a PB-10 Basic pH Meter (Shanghai Sartorius Trading Co. Ltd., China) and a DDS-307A Conductivity Meter (Shanghai Inesa Scientific Instrument Co., Ltd., China), respectively. The Futura TM Continuous Flow Analytical System (Alliance Instruments, France) was used to quantify the ammonium nitrogen (NH 4 + -N), the nitrate-nitrogen (NO 3 -N) and available phosphorus using the method described by Shitan Bao [27]. The organic matter content was determined using the dichromate digestion method [26].

Strawberry Growth, Mortality and Yield
Strawberry growth index was evaluated in both trial sites. Lack of funding resulted in the strawberry yield being monitored in Trial I only. After 120 days of strawberry transplant, 40 strawberry plants were randomly selected from each plot to measure stem thickness and plant height using the Vernier calipers and rulers. Two rows of strawberries were randomly selected from each plot to collect the yield data during the harvesting season (February 2018) in Trial I, and the yield of each row was recorded with each harvest. In May 2018, the mortality caused by strawberry blight was determined from two randomly selected rows from each plot.
The Chinese Traditional Spring Festival was in February in 2018. During this period, the selling price of strawberries is at its highest point. Therefore, in the experiment, we determined the highest average price of "Red Face" at this time (USD 4.4 kg −1 ), based in 2018 Beijing Xinfadi agricultural product market price forecast (http://www.xinfadi.com.cn), to calculate the grower's income.

DNA Extraction and HTS Analysis
DNA was extracted and purified from samples of 0.25 g of soils using the MoBio PowerSoil ® DNA Isolation Kit (MoBio Technologies Inc., USA) following the manufacturer's instructions. After extraction, the quality of the DNA was evaluated by electrophoresis on a 0.5% (w/v) agarose gel. The concentration of the DNA samples was measured by a NanoDrop TM 1000 spectrophotometer (Thermo Fisher Scientific Inc., USA).
The illumina adapters 5 -AATGATACGGCGACCACCGAGATCTACAC-3 and 5 -CAAGCAGAAGACGGCATACGAGATGCCGCATTCGAT-3 were used. The polymerase chain reaction (PCR) amplifications were performed, recovered and quantified for each sample in accordance with the procedure described in a previous study [2]. The average length of the paired-end sequencing of bacteria and fungi PCR products was 417 bp and 245 bp, respectively. The sequencing was performed using the Illumina Miseq system of Majorbio Bio-Pharm Technology Co., Ltd. (Shanghai, China). The sequencing procedure has been described in a previous study [2].
Majorbio Bio-Pharm Technology Co., Ltd. (Shanghai, China) analyzed the abundance and community structure of bacteria and fungi in the soil on the basis of the standard protocols described in the purified amplicons method with the Illumina MiSeq platform [30]. The Quantitative Insights Into Microbial Ecology (QIIME I 1.9.1) open-source bioinformatics pipeline was used for the post-sequencing analysis [31]. First, the sequences obtained were clustered into operational taxonomic units (OTUs) based on a 97% similarity threshold with the UPARSE Pipeline v 7.0. The effective sequence depth was normalized to compare all samples at the same sequencing depth. On the basis of the cluster analysis, Sustainability 2020, 12, 6313 5 of 15 the alpha diversity indices (e.g., Chao and Shannon) were calculated and further compared by one-way ANOVA test (p ≤ 0.05) with the SPSS statistical software. Significant differences among means were evaluated using Tukey's HSD test at p = 0.05. The taxonomy of each OTU's representative sequence was analyzed using the RDP Classifier (http://rdp.cme.msu.edu/). The OTUs' representative sequences were compared to the 16S rRNA database on the bacteria Silva (Release128 http://www.arb-silva.de) and to the fungi ITS database on the Unite (Release 7.0 http://unite.ut.ee/index.php). The Bray-Curtis method was used to generate the Principal Coordinates Analysis (PCoA). The differences between groups of microbial diversity were tested by the Adonis method, and the number of replacements was set to 999. The statistical analysis was performed using the one-way ANOVA test (p ≤ 0.05), and Falsely Discovery Rate (FDR) correction was used for the multiple correction test.

Data Analysis
The strawberry mortality rate (X, %) was calculated using the Equation (1): where X is the strawberry mortality (%), N1 is the number of dead strawberry plants in the two selected rows of each plot, and N2 is the number of live strawberry plants in the two selected rows of each plot. The inhibition of soil-borne fungi pathogens by counting CFU (Equation (2)) was used to determine the control efficacy of ASD in relation to Fusarium spp. and Phytophthora spp. (based on CFU counting): where Y is the inhibition rate of fungi growth based on the counting CFU (%), Au is the number of fungal colonies in the untreated control, and At is the number of fungal colonies in the treatment. Field trial data (including strawberry plant height, stem thickness, mortality, yield and output value) and the soil's physical and chemical properties were analyzed using a one-way analysis of variance (ANOVA) by the SPSS of Windows v. 22.0 statistical software. The variance was analyzed after the strawberry plant growth and mortality data in percentages had been normalized through arcsine conversion. The yield and the data on the soil's physical and chemical properties were analyzed directly without any normalization. Significant differences among means were evaluated using Tukey's HSD test at p = 0.05 [32]. The figures were drawn with the OriginPro ® 9.0 (OriginLab Corp., USA) and Adobe Illustrator ® CS6 (Adobe Systems Inc., USA) mapping software.

Fungi Soil-Borne Pathogens
The results showed that the CCM 6 and the CCM 12 treatments significantly reduced the colonies of Fusarium spp. and Phytophthora spp. at the 0-20 cm depth of the soil, compared to the CK after 30 days of soil disinfestation ( Figure 1). The ASD treatment reduced Fusarium spp. and Phytophthora spp. by 91% and 94%, respectively, in the two trials. Nevertheless, the colonies of Fusarium spp and Phytophthora spp were recovered after 120 days, despite ASD treatment. ASD significantly increased the CFU of Fusarium spp. compared to CK in the two trials after 120 days. However, in the CCM 12 treatment, Fusarium spp. grew back to the same levels as in CK in Trial II. The quantity of Phytophthora spp. colonies recovered to the same level as they were at with CK in the two trials, except with the CCM 12 treatment, which maintained suppression in Trial I.

Soil Physicochemical Properties
The soil ammonium nitrogen, soil nitrate-nitrogen and OM increased significantly after 30 days of ASD treatments, compared to the CK, and there was no difference between the CCM 6 and the CCM 12 (Table 3). The oxidation-reduction potential of the ASD treatments was significantly less (182 and 171 mv) than with CK (213 mv). In addition, the available potassium of the CCM 12 treatment was significantly higher (882 mg kg −1 ) than that of CK (750 mg kg −1 ). However, CCM 6 (773 mg kg −1 ) did not show a significant difference from CK.

Soil Physicochemical Properties
The soil ammonium nitrogen, soil nitrate-nitrogen and OM increased significantly after 30 days of ASD treatments, compared to the CK, and there was no difference between the CCM 6 and the CCM 12 ( Table 3). The oxidation-reduction potential of the ASD treatments was significantly less (182 and 171 mv) than with CK (213 mv). In addition, the available potassium of the CCM 12 treatment was significantly higher (882 mg kg −1 ) than that of CK (750 mg kg −1 ). However, CCM 6 (773 mg kg −1 ) did not show a significant difference from CK.

Strawberry Growth, Mortality Rate and Yield
In the present study, the strawberry plant height and stem thickness were significantly greater with soil fumigation than they were in CK in the two trials, except CCM 6 did not show a significant difference from the control in Trial I (Figure 2A-D). At the end of the experiment, CCM 6 (3.2%, 4.1%) and CCM 12 (2.5%, 2.9%) treatments significantly reduced strawberry mortality in the two trials, compared to CK (12.4%, 15.8%) ( Figure 2E,F). Strawberry yields were 5.0 and 5.3 tons ha −1 with CCM 6 and CCM 12 disinfestation, respectively, doubling the yield obtained with CK (2.5 ton kg −1 ) in Trial I ( Figure 2G). The strawberry yield with the CCM 12 treatment was significantly higher than the CCM 6 in Trial I.  The increased cost of ASD is negligible compared to the benefits of increased strawberry production. CCM 6 and CCM 12 could significantly increase strawberry-based economic income by USD 10,937-12,449 ha −1 in February for growers ( Figure 2H). The costs of CCM 6 and CCM 12 were USD 4969 and 5669 ha −1 , respectively (the cost of TIF is USD 4285 ha −1 , and the cost of CCM is USD 114 ha −1 ). The net increases in income from strawberry production were USD 5968 and 6780 ha −1 after ASD fumigation in February, for CCM 6 and CCM 12, respectively.

Soil Microbial Diversity Analysis
The HTS for bacteria yielded 590,359 raw sequences clustered in 7742 OTUs (Figure 3a). For fungi, 1299 OTUs were clustered from 781,886 raw sequences, all generated from all soil samples after ASD fumigation (Figure 3b). manure, CCM 12 = 12 tons/ha composted chicken manure, CK = untreated control. The strawberry output value was for one month (February 2018). We use the highest average price of the "Hongyan" strawberry (USD 4.43 kg −1 ), based on the Beijing Xinfadi agricultural product market price forecast for February 2018 (http://www.xinfadi.com.cn) to calculate the strawberry output value. Error bars represent the standard errors of means (n = 4). Different letters mean significant differences between treatments. Data were analyzed for ANOVA. Duncan's new multiple range test was used to determine statistical differences (p ≤ 0.05).

Soil Microbial Diversity Analysis
The HTS for bacteria yielded 590,359 raw sequences clustered in 7742 OTUs (Figure 3a). For fungi, 1299 OTUs were clustered from 781,886 raw sequences, all generated from all soil samples after ASD fumigation (Figure 3b). In the study, CCM 6 (Chao index, p = 0.016; Shannon index, p = 0.023) increased the bacterial richness and diversity compared to CK. At a higher OM level, the increase was significantly less ( Figure 4A,C). The fungal diversity showed no difference between all treatments ( Figure 4B). However, CCM 6 (Chao index, p = 0.023) led to a higher fungal richness than the other treatments ( Figure 4D). In the study, CCM 6 (Chao index, p = 0.016; Shannon index, p = 0.023) increased the bacterial richness and diversity compared to CK. At a higher OM level, the increase was significantly less ( Figure 4A,C). The fungal diversity showed no difference between all treatments ( Figure 4B). However, CCM 6 (Chao index, p = 0.023) led to a higher fungal richness than the other treatments ( Figure 4D).  . Different letters mean significant differences between treatments. Data were analyzed for Student's t-test. Significant differences among means were evaluated using the Tukey's HSD test at p = 0.05.
Based on the PCoA analysis, the results suggest that ASD treatments changed the soil bacterial and fungal microbial communities' compositions compared to CK (Figure 5). At the soil bacterial phyla level (Figure 6a), ASD fumigation significantly increased the relative abundance of OTUs, classified into Chloroflexi (p = 0.02274) and Bacteroidetes (p = 03414), compared to CK. Thirty-one soil bacterial genera showed significant differences between treated and untreated samples (Figure 6b). The relative abundance of bacteria genera from the families of Anaerolineaceae, Rhodothermaceae and Caldilineaceae, and the genera Chryseolinea and Anaeromyxobacter, significantly increased with soil fumigation. ASD fumigation greatly reduced the relative abundance of Bacillus, Streptomyces, Defluviicoccus and Enhygromyxa.  Different letters mean significant differences between treatments. Data were analyzed for Student's t-test. Significant differences among means were evaluated using the Tukey's HSD test at p = 0.05. Based on the PCoA analysis, the results suggest that ASD treatments changed the soil bacterial and fungal microbial communities' compositions compared to CK (Figure 5). At the soil bacterial phyla level (Figure 6a), ASD fumigation significantly increased the relative abundance of OTUs, classified into Chloroflexi (p = 0.02274) and Bacteroidetes (p = 03414), compared to CK. Thirty-one soil bacterial genera showed significant differences between treated and untreated samples (Figure 6b). The relative abundance of bacteria genera from the families of Anaerolineaceae, Rhodothermaceae and Caldilineaceae, and the genera Chryseolinea and Anaeromyxobacter, significantly increased with soil fumigation. ASD fumigation greatly reduced the relative abundance of Bacillus, Streptomyces, Defluviicoccus and Enhygromyxa.  At the fungal phyla level (Figure 7a), compared with CK, the CCM 12 treatment significantly reduced the relative abundance of the genus Fusarium spp. (p = 0.0220) by 54% (Figure 7b). However, the ASD fumigation methods significantly increased the beneficial fungi Trichoderma spp. (p = 0.0366) by 3055% and 1600%, respectively (p < 0.05).   Comparison of relative abundances of the soil bacteria phyla (a) and genera (b) detected in histograms of the various soil microbial compositions exposed among different treatment groups. CCM 6 = 6 tons/ha composted chicken manure, CCM 12 = 12 tons/ha composted chicken manure, CK = untreated control. The number of asterisks indicates a significant difference between treatments in accordance with the one-way ANOVA test (p ≤ 0.05). Falsely Discovery Rate (FDR) correction was used for the multiple correction test, and Scheffe was used for post-hoc tests.

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Commented [sz29R sentence. Figure 6. Comparison of relative abundances of the soil bacteria phyla (a) and genera (b) detected in histograms of the various soil microbial compositions exposed among different treatment groups. CCM 6 = 6 tons/ha composted chicken manure, CCM 12 = 12 tons/ha composted chicken manure, CK = untreated control. The number of asterisks indicates a significant difference between treatments in accordance with the one-way ANOVA test (p ≤ 0.05). Falsely Discovery Rate (FDR) correction was used for the multiple correction test, and Scheffe was used for post-hoc tests.
Sustainability 2020, 12, x FOR PEER REVIEW 12 of 17 Figure 7. Comparison of relative abundances of the soil fungal phyla (a) and genera (b) detected in histograms of the various soil microbial compositions exposed among the different treatment groups. CCM 6 = 6 tons/ha composted chicken manure, CCM 12 = 12 tons/ha composted chicken manure, CK = untreated control. The number of asterisks indicates a significant difference between treatments in accordance with the one-way ANOVA test (p ≤ 0.05). Falsely Discovery Rate (FDR) correction was used for the multiple correction test, and Scheffe was used for post-hoc tests.

Effect of ASD on Fungal Pathogens
ASD's efficacy against soil pathogens can be variable, and depends on the target pathogens in the soil and the used carbon source [17]. In our field trials, the results showed that ASD had a highly suppressive effect on Fusarium spp. and Phytophthora spp. (Figure 1). The test results from the two experimental trials remained consistent (Figure 1a-d). In another case study, Termorshuizen reported that the compost suppressive activity in the pathosystems Fusarium oxysporum on flax and Phytophthora nicotianae on tomato was otherwise conducive in the Verticillium dahlia-eggplant combination [33]. Ammonia and nitrous acid production has been identified as a mechanism for pathogen and root-knot nematode control, with the use of high-nitrogen inputs alone (including composted or un-composted animal manure) [34] or in combination with solarization effects [35]. In general, mixed compost has a better disease suppression effect on pathogenic bacteria than pure compost [36]. In our experiments, however, the pathogens recovered their original levels 120 days after treatment. Liu et al. showed that F. oxysporum, in watermelon cultivation, regenerated 90 days after ASD disinfestation, and this conclusion is consistent with our results [37].

Effect of ASD on the Soil Physicochemical Properties
Various organic amendments can help improve soil quality [38,39]. ASD using CCM can significantly increase the content of ammonium nitrogen and OM in the soil, and significantly reduce the content of nitrate-nitrogen, which indicates that the ASD process will affect the mineralization of soil nitrogen. In most studies (73%, n = 426), OM addition is either effective in, or detrimental to, the inhibition of specific pathogens in the soil. The decomposition of OM is the key process affecting inhibition in the short-term (days or weeks) or the long-term (months or years [17,40]). Butler et al. (2014) reported that ASD-treated soils are reductive, with lower oxidation-reduction potential than the control [17].

Effect of ASD on Strawberry Growth and Yield
The results showed that strawberry plants grown from compost-fertilized plots exhibited generally higher and thicker stems, and better fruit weights. Animal manure and compost are organic  Comparison of relative abundances of the soil fungal phyla (a) and genera (b) detected in histograms of the various soil microbial compositions exposed among the different treatment groups. CCM 6 = 6 tons/ha composted chicken manure, CCM 12 = 12 tons/ha composted chicken manure, CK = untreated control. The number of asterisks indicates a significant difference between treatments in accordance with the one-way ANOVA test (p ≤ 0.05). Falsely Discovery Rate (FDR) correction was used for the multiple correction test, and Scheffe was used for post-hoc tests.

Effect of ASD on Fungal Pathogens
ASD's efficacy against soil pathogens can be variable, and depends on the target pathogens in the soil and the used carbon source [17]. In our field trials, the results showed that ASD had a highly suppressive effect on Fusarium spp. and Phytophthora spp. (Figure 1). The test results from the two experimental trials remained consistent (Figure 1a-d). In another case study, Termorshuizen reported that the compost suppressive activity in the pathosystems Fusarium oxysporum on flax and Phytophthora nicotianae on tomato was otherwise conducive in the Verticillium dahlia-eggplant combination [33]. Ammonia and nitrous acid production has been identified as a mechanism for pathogen and root-knot nematode control, with the use of high-nitrogen inputs alone (including composted or un-composted animal manure) [34] or in combination with solarization effects [35]. In general, mixed compost has a better disease suppression effect on pathogenic bacteria than pure compost [36]. In our experiments, however, the pathogens recovered their original levels 120 days after treatment. Liu et al. showed that F. oxysporum, in watermelon cultivation, regenerated 90 days after ASD disinfestation, and this conclusion is consistent with our results [37].

Effect of ASD on the Soil Physicochemical Properties
Various organic amendments can help improve soil quality [38,39]. ASD using CCM can significantly increase the content of ammonium nitrogen and OM in the soil, and significantly reduce the content of nitrate-nitrogen, which indicates that the ASD process will affect the mineralization of soil nitrogen. In most studies (73%, n = 426), OM addition is either effective in, or detrimental to, the inhibition of specific pathogens in the soil. The decomposition of OM is the key process affecting inhibition in the short-term (days or weeks) or the long-term (months or years [17,40]). Butler et al. (2014) reported that ASD-treated soils are reductive, with lower oxidation-reduction potential than the control [17].

Effect of ASD on Strawberry Growth and Yield
The results showed that strawberry plants grown from compost-fertilized plots exhibited generally higher and thicker stems, and better fruit weights. Animal manure and compost are organic sources of nutrients, and have been widely demonstrated to increase the soil OM, improve soil quality and increase crop yield [13]. Research has shown that the leaf areas, the number of flowers, the branch weight and the total strawberry yield could be increased significantly in a plot treated with compost, compared to in plots that receive only inorganic fertilizers [41].
These results suggest that the strawberry yield could improve when the dose of CCM increases. For effective disease suppression, relatively high rates of amendment incorporation are reported as necessary by Mowlick [13,42]. The research has shown that, with the increase in the amount of chicken manure in the field, the growth parameters of several vegetables and corn plants increased significantly [43]. The yield of the strawberry fruit from plants grown in CCM 12-treated soil was significantly (7%) higher than that grown in CCM 6-treated soil. The CCM 12 costs USD 695 ha −1 more than CCM 6, which is calculated from composting chicken manure costing USD 114 ha −1 . However, strawberry farmers can increase profits by up to USD 1512 ha −1 just based on the strawberry harvest in February.

Effect of ASD on Soil Microbial Diversity and Community
CCM 6 had a significant impact on the bacterial and fungal diversity and richness after ASD fumigation. ASD effectivity is influenced by the production of volatile organic compounds and organic acids, lethal anaerobic conditions, and changes in soil microbial communities [14]. The ASD treatments tend to increase the Chao and Shannon indices of bacterial and fungal communities in the soil. In the literature, the Shannon-Wiener index for soil without fumigation was significantly lower than that of the ASD-treated and flooded soil, incorporated with 1.6% (w/w) pig manure [44]. Butler used a partially composted poultry litter amendment for ASD, which could potentially improve the soil microbial diversity of low organic soils [11].
ASD fumigation affected the fungal and bacterial community taxonomy, leading to the abundance of functional microbial communities such as anaerobic and organic matter decomposition. In the current study, ASD fumigation increased the relative abundance of bacteria g__norank_f__Anaerolineaceae, norank_f__Rhodothermaceae and Anaerolinea (Figure 6a and Figure S1). Anaerolineaceae spp. play a key role in the initial activation of long-chain n-alkane biodegradation [45]. Anaerolinea spp. are a strictly anaerobic Gram-negative bacteria that can ferment sugar to produce acetic acid and hydrogen, and can utilize lactate, acetate and ethanol [46]. The growth of aerobic microorganisms consumes the remaining soil oxygen, and gives rise to anaerobic soil conditions [37,[47][48][49]. The anaerobic bacteria that were grown in the biological disinfestation-treated soil play an important role in significantly degrading the Fusarium pathogen [50]. ASD fumigation reduced the relative abundance of Bacillus and Streptomyces in the soil (Figure 6b). An antagonistic strain of Bacillus subtilis Y-IVI was reported to control the Fusarium wilt caused by Fusarium oxysporum f. sp. Melonis [51]. According to the literature, plant-pathogenic Streptomyces species can cause parasitic plant diseases in the underground structures of diverse plant species. For example, Streptomyces scabiei infects the underground tubers of potatoes, causing common scab disease [52].
The CCM 12 treatment significantly reduced the abundance of the fungi Fusarium (Figure 7b). Ueki et al. showed that two anaerobic, isolates identified as Clostridium beijerinckii, had severely degraded the mycelial cells of the Fusarium pathogen of spinach wilt disease during anaerobic co-incubation [50]. Furthermore, the CCM 6 and the CCM 12 treatments increased the beneficial fungi Trichoderma (Figure 7b). Since the 1920s, Trichoderma has been known for its biocontrol of plant pathogens [53]. A meta-analysis showed that ASD promotes the population of the Trichoderma [13].

Conclusions
The results of this study show that the CCM and TIF can play an important role in ASD's suppressive effect on soil-borne diseases. The fumigation efficacy with ASD increased, and the responses in strawberry yield, soil nutrition and soil microbial communities were modified, compared to the control. The colonies of Fusarium spp. and Phytophthora spp., which are recognized to cause strawberry soil-borne diseases, decreased significantly after ASD. The marketable income from strawberries was greater after ASD disinfestation, and this economic benefit could be due to the suppression of soil-borne diseases and the improvement of soil nutrition.
The research monitored the changes of the bacterial and fungal communities' relative compositions after ASD fumigation. In brief, our research observed that ASD significantly increased the richness of the soil bacterial and fungal community, and significantly increased the proportions of the Anaerolineaceae family, and more specifically the Anaerolinea genus, which could be vital under the lethal anaerobic condition. The proportion of the beneficial fungi Trichoderma spp. increased significantly with soil treatment, and this could lead to a pest and disease incidence reduction during the production of strawberries. The improved efficacy of ASD may result from the TIF, which created a significantly anaerobic soil condition. In their study, the authors of [54] showed that TIF not only reduces emissions, but also improves efficacy, with better fumigant-retention under the film.
Future research is needed in order to continue addressing the challenges of soil-borne disease-control in different types of soil, and cover type for ASD technology for crops. In addition, the type of compost and application amount both need to be evaluated separately in order to expand the application, and beneficial microbial agents can be added to enhance the effects of ASD.