Recent Progress of Copper-Based Nanomaterials in Tumor-Targeted Photothermal Therapy/Photodynamic Therapy

Nanotechnology, an emerging and promising therapeutic tool, may improve the effectiveness of phototherapy (PT) in antitumor therapy because of the development of nanomaterials (NMs) with light-absorbing properties. The tumor-targeted PTs, such as photothermal therapy (PTT) and photodynamic therapy (PDT), transform light energy into heat and produce reactive oxygen species (ROS) that accumulate at the tumor site. The increase in ROS levels induces oxidative stress (OS) during carcinogenesis and disease development. Because of the localized surface plasmon resonance (LSPR) feature of copper (Cu), a vital trace element in the human body, Cu-based NMs can exhibit good near-infrared (NIR) absorption and excellent photothermal properties. In the tumor microenvironment (TME), Cu2+ combines with H2O2 to produce O2 that is reduced to Cu1+ by glutathione (GSH), causing a Fenton-like reaction that reduces tumor hypoxia and simultaneously generates ROS to eliminate tumor cells in conjunction with PTT/PDT. Compared with other therapeutic modalities, PTT/PDT can precisely target tumor location to kill tumor cells. Moreover, multiple treatment modalities can be combined with PTT/PDT to treat a tumor using Cu-based NMs. Herein, we reviewed and briefly summarized the mechanisms of actions of tumor-targeted PTT/PDT and the role of Cu, generated from Cu-based NMs, in PTs. Furthermore, we described the Cu-based NMs used in PTT/PDT applications.


Introduction
Cancer is a complicated disease characterized by various genetic flaws.The increased incidence of malignant tumors and their predisposition for metastasis are major threats to human health, which lead to higher mortality rates [1,2].The traditional treatment modalities for tumors, including surgery, radiotherapy, and chemotherapy, can engender various adverse effects, including radioactive damage, toxic side effects of chemotherapeutic drugs, or chemotherapy-induced multidrug resistance, which considerably limits the therapeutic effectiveness of these modalities [3][4][5][6].The emerging treatment modalities for tumors, such as thermotherapy, immunotherapy, and gene therapy, have provided greater hope for patients [7,8].However, their prolonged use has been reported to severely impair the immune system, even leading to organ malfunction [9,10].Discovering a tumor treatment modality with low toxicity to healthy tissues and improved precision presents an imperative and formidable challenge.
Recently, phototherapies (PTs), such as photothermal therapy (PTT) and photodynamic therapy (PDT), are being widely used as effective antitumor therapeutic strategies [10].In PTT and PDT, photothermal agents (PAs) and photosensitizers (PSs) work as exogenous energy converters or absorbers in the organ affected by the tumor [11].This has enabled the conversion of visible or laser light energy into thermal energy to induce apoptosis or necrosis of tumor cells at high temperatures, with or without concurrent reactive oxygen species (ROS) generation [12][13][14][15].PT has specific advantages over other heat-based tumor treatment techniques, including thermotherapy and microwave therapy.Its high precision enables PT to target tumors with the least possible damage to adjacent tissues and organs [16].Additionally, PT is an excellent method for managing tumors because of its controllability and low toxicity profile.Compared with either PTT or PDT acting alone, the synergistic approach combining PTT and PDT can hasten tumor cell death and increase the therapeutic efficacy [17].The key components of PTT and PDT are their PAs and PSs, majorly including nanomaterials (NMs) at present, which differ from conventional macromolecules.NMs exhibit excellent characteristics, including high Brunauer-Emmett-Teller (BET), electrical conductivity, spectrum shifts after light absorption, fluorescence properties, and potential for degradation [18].Therefore, they have been steadily incorporated into cancer research, advancing the field of tumor investigations [19][20][21].Regarding medical therapeutics, materials of nanoscale dimensions (1-100 nm diameter) have been employed [22].These NMs have versatile applications in drug transportation, enabling controlled release mechanisms, increasing permeability, traversing biological barriers, and improving overall biocompatibility [23][24][25].Owing to their unique size and traits, NMs can remarkably change various therapeutic processes and considerably improve treatment efficacy [22].Metal ion-based NMs, including those of gold (Au), silver (Ag), copper (Cu), and other NMs, exhibit distinctive optical properties, notably the phenomenon of localized surface plasmon resonance (LSPR) [26,27].LSPR notably strengthens the electric field in the immediate proximity of metal ion-based NMs, based on their capacity to strongly absorb photon energy.Owing to an extensive range of light-induced effects and intermolecular interactions produced, they are used in tumor-targeted PTT/PDT.However, the high cost of Au-or Ag-based NMs and the non-degradability of some PAs/PSs in vivo limits their application in clinical therapy [28].Cu, a readily available metal, has unique bioactivities that can be used for eliminating tumor cells by regulating various types of cell death [29].Furthermore, Cu can undergo a Fenton-like reaction to catalyze high ROS content from excess intracellular hydrogen peroxide (H 2 O 2 ), which can induce bacterial and tumor cell death [30].The functions and therapeutic involvement of Cu are shown in Figure 1.Al Kayal et al. created electrospun polyurethane membranes covered with Cu nanoparticles (NPs) and ran an antimicrobial test on Escherichia coli and discovered that over half of the bacteria were destroyed, showing its potent bactericidal effects.Additionally, SARS-CoVer-2 was resistant to the antiviral activity by nearly 90% [31].Cu-based NMs have been widely employed in PTT and PDT in recent years because of their advantageous properties, including strong near-infrared (NIR) absorption and photothermal capabilities [32,33], high BET [34,35], and use in tumor imaging.[36] Furthermore, Cu-based NMs offer considerable advantages in tumor therapy owing to their simple synthesis procedures and relatively high production yields under mild low reaction conditions [37].Zhao et al. synthesized CCeT NMs (Ce: Chlorin e6, T: TPP-COOH) with Cu 2−x Sulfur (S) as the core, which exhibited a photothermal conversion efficiency (PCE) of 10.6% under laser irradiation.At very low concentrations, the temperature increased by 5.3 • C in 10 min and continued to rise with increasing concentration [38], demonstrating the therapeutic advantages of PTT/PDT and that it can be substantially increased using Cu-based NMs.
Herein, we reviewed Cu-based NMs and explored the role of Cu in synergizing PTT/PDT for tumor cell death.This review also provides a comprehensive overview of the Cu-based NMs utilized in PTT/PDT.PTT can be divided into traditional PTT (≥45 °C) and mild PTT (MPTT, 42-45 °C) [39].It is an antitumor therapy where PAs convert laser light energy into thermal energy under NIR irradiation, raising the temperature of the surrounding region and inducing apoptosis or necrosis in the tumor cells [40].In PDT, PSs generate ROS in the presence of oxygen molecules upon or following photoirradiating of the tumor tissue, resulting in oxidative damage-induced tumor cell death [41].PDT is often classified as type I or type II, where type I produce O2 − , hydroxyl radicals (•OH), and H2O2, and type II produce 1 O2 [42,43].Although PTT and PDT may both cause tumor cell death by phototoxicity and producing ROS, they still have notable drawbacks in actual use [44].
First, the practical usage of PTT/PDT is generally constrained by PAs/PSs because of the low tissue penetration of light, which leads to poor tumor cell death, tumor resistance to therapy, and possible excruciating pain during treatment [45,46].Additionally, with the rise in local temperature around the tumor, adjacent normal tissues and cells may also suffer damage.Finally, ROS production is dependent on the oxygen present around the tissue, but the low oxygen content in the tumor microenvironment (TME) severely restricts the effectiveness of PDT [47].To resolve these problems, NMs are incorporated into PTT and PDT; Cu-based NMs can act as carriers or modified PAs or PSs to fix the limitations of conventional PAs or PSs [43,48].For example, Zhang et al. produced biodegradable cancer cell membrane-coated mesoporous Cu/manganese (Mn) silicate nanospheres (m@CMSNs) to target the aggregation of homozygous cancer cells via adhesion molecules on the surface of cancer cell membranes [49,50].Under 635 nm laser irradiation, m@CMSNs were targeted to aggregate 2.85-fold more than CMSNs at the tumor site and degraded by glutathione (GSH) in the TME.Cu 1+ functions as a very effective PSs, producing huge levels of 1 O2 at the tumor site, killing tumor cells and reducing tumor cell viability to 20% [51].Thus, the deficiencies of conventional PAs/PSs, which are especially addressed in detail in the next section, can be significantly improved by Cu-based NMs.C) [39].It is an antitumor therapy where PAs convert laser light energy into thermal energy under NIR irradiation, raising the temperature of the surrounding region and inducing apoptosis or necrosis in the tumor cells [40].In PDT, PSs generate ROS in the presence of oxygen molecules upon or following photoirradiating of the tumor tissue, resulting in oxidative damage-induced tumor cell death [41].PDT is often classified as type I or type II, where type I produce O 2 − , hydroxyl radicals (•OH), and H 2 O 2 , and type II produce 1 O 2 [42,43].Although PTT and PDT may both cause tumor cell death by phototoxicity and producing ROS, they still have notable drawbacks in actual use [44].
First, the practical usage of PTT/PDT is generally constrained by PAs/PSs because of the low tissue penetration of light, which leads to poor tumor cell death, tumor resistance to therapy, and possible excruciating pain during treatment [45,46].Additionally, with the rise in local temperature around the tumor, adjacent normal tissues and cells may also suffer damage.Finally, ROS production is dependent on the oxygen present around the tissue, but the low oxygen content in the tumor microenvironment (TME) severely restricts the effectiveness of PDT [47].To resolve these problems, NMs are incorporated into PTT and PDT; Cu-based NMs can act as carriers or modified PAs or PSs to fix the limitations of conventional PAs or PSs [43,48].For example, Zhang et al. produced biodegradable cancer cell membrane-coated mesoporous Cu/manganese (Mn) silicate nanospheres (m@CMSNs) to target the aggregation of homozygous cancer cells via adhesion molecules on the surface of cancer cell membranes [49,50].Under 635 nm laser irradiation, m@CMSNs were targeted to aggregate 2.85-fold more than CMSNs at the tumor site and degraded by glutathione (GSH) in the TME.Cu 1+ functions as a very effective PSs, producing huge levels of 1 O 2 at the tumor site, killing tumor cells and reducing tumor cell viability to 20% [51].Thus, the deficiencies of conventional PAs/PSs, which are especially addressed in detail in the next section, can be significantly improved by Cu-based NMs.

Biological Properties of Cu and Benefits of Cu-Based NMs
Cu is the third most common essential trace element after zinc and iron [52].Despite being in low concentrations, Cu is widely distributed in biological tissues and plays roles in many critical processes, including cellular respiration, energy metabolism, and ROS removal [53,54].Both Cu deficit and surplus conditions can cause serious diseases [52,55].Several studies have shown increased Cu levels (2-3 times higher) in patients with tumors compared with that in healthy individuals [56,57].Hence, the strict regulation of Cu levels in cells and tissues is essential.
Owing to the special biological characteristics of Cu, Cu-based NMs provide many benefits in antitumor therapy: (1) Cu-based NMs have favorable NIR absorption and excellent photothermal performance because of the d-d transition of Cu 2+ , the unpairing of 3d electrons and paramagnetic of Cu 2+ [58].They also demonstrate the capability of magnetic resonance imaging (MRI), which has been widely used in PTT and photoacoustic imaging (PAI) of tumors [59,60].Because Cu 2+ in small Cu sulfide (S) NPs (s-Cu 2-x S NPs) have unpaired electrons, they are magnetic and can be employed as possible contrast agents for T1-weighted MRI [61].Chen et al. synthesized ultrasmall structured u-Cu 2-x S (Cu 2+ ) nanodots and found an increase in the photoaccoustic signal in mice, especially at the tumor site.In particular, the PA signals could sustain markedly higher intensities for extended periods [62].Weitz  Li et al. synthesized GSH-degrading CFT@IP6@BSANPs (CFT: Cu iron tellurite, IP6: inositol hexaphosphate, BSA: bovine serum albumin) that effectively degraded overexpressed GSH in the TME.The simultaneous release of Cu 1+ loaded within the NMs, which acted as chemotherapeutic agents, induced tumor cell deaths [68].(4) Cu-based NMs can produce high ROS quantities when exposed to light; therefore, they are frequently exploited as PAs/PSs for PTT/PDT of malignancies.Qu et al. synthesized hollow CuS nanocubes that increased temperature in a concentration-dependent manner under 808 nm laser irradiation, with a PCE of 30.3%; additionally, increased ROS generation was detected which induced tumor cell deaths, acting as an antitumor therapy [69].Therefore, Cu-based NMs with exceptional characteristics have been successfully created to be used in the combined treatment of malignancies to give Cu-based NMs extra functionalities.PTT/PDT and Cu-based NMs can both be used to treat cancer in different ways and Cu-based NMs can compensate for some of the drawbacks of PTT/PDT.Thus, Cu may synergize with PTT/PDT to induce tumor cell death in various ways to achieve antitumor effects. 1 O 2 oxidizes unsaturated fatty acid constituents of the membrane to malondialdehyde, damaging the integrity of the membrane which causes rapid utilization of intracellular adenosine triphosphate (ATP), inducing necrosis [71,72].Proteins and lipids, which make up the majority of the tumor cell membrane, are photosensitive and their chemical transformation may swiftly destroy cells, even when light is administered at low temperatures.Necrotic tumor cells can attract antigen-presenting cells and deliver tumor-associated antigens to naive T cells [73], which leads to the activation of cytotoxic T cells present near the tumor tissue and damage-associated molecular patterns (DAMPs) from dying tumor cells, inducing the immune response [74].After light irradiation and tumor cell death, phosphatidylserine may present to macrophages, inducing immunosuppressive cytokine generation, which inhibit the growth of antigen-presenting dendritic cells (DCs) and reduces inflammation [75].Additionally, DCs are further activated by DAMPs, which stimulate the immune system and initiate antitumor immunological responses, increasing their capacity to uptake tumor-associated antigenic epitopes [76].Jin  to tumor cell necrosis [70].Cu has a photothermal ability and a high NIR absorption ca pacity; therefore, Cu-containing PSs/PAs localized in the tumor cell membrane interac with the surrounding oxygen molecules during NIR irradiation, leading to an elevate energy level and the formation of 1 O2 through oxygen energy transfer.Then, 1 O2 oxidize unsaturated fa y acid constituents of the membrane to malondialdehyde, damaging th integrity of the membrane which causes rapid utilization of intracellular adenosine tr phosphate (ATP), inducing necrosis [71,72].Proteins and lipids, which make up the ma jority of the tumor cell membrane, are photosensitive and their chemical transformatio may swiftly destroy cells, even when light is administered at low temperatures.Necrot tumor cells can a ract antigen-presenting cells and deliver tumor-associated antigens t naive T cells [73], which leads to the activation of cytotoxic T cells present near the tumo tissue and damage-associated molecular pa erns (DAMPs) from dying tumor cells, in ducing the immune response [74].After light irradiation and tumor cell death, phospha tidylserine may present to macrophages, inducing immunosuppressive cytokine genera tion, which inhibit the growth of antigen-presenting dendritic cells (DCs) and reduces in flammation [75].Additionally, DCs are further activated by DAMPs, which stimulate th immune system and initiate antitumor immunological responses, increasing their capac ity to uptake tumor-associated antigenic epitopes [76].Jin

Cu-Based NMs Synergizes PTT/PDT-Induced Apoptosis
Cu is a crucial trace element for the organism and several studies have shown its harmful effects.
In a study, a Cu-based treatment of apoptotic factors increased B-cell leukemia/lymphoma-2-associated X-protein (Bax), B-cell leukemia/lymphoma-2associated agonist of cell death protein (Bad), cytochrome c (Cyt c), and caspases 3 and 9 levels [79].By binding to apoptosis-activating caspase 9, the Cu induced an increase in Bax level, causing the release of Cyt c and mitochondrial apoptosis-inducing factor 1 (Apaf-1) from the mitochondria into the cytoplasm, where it activated caspase 3 to cause apoptosis.Simultaneously, ROS caused DNA breakage in PC12 cells and activated the caspase cascade response [79].Additionally, Cu-induced ROS increased lipid peroxidation and decreased GSH levels, making cells more vulnerable to oxidative stress-induced harm [80,81].Hosseini et al. reported that Cu 2+ interacted with respiratory complexes (I, II, and IV) and promoted ROS generation in mouse hepatocytes in vitro [82].Mitochondria is also an important target for PAs/PSs.Hilf showed that PSs enter tumor cells and are rapidly taken up by mitochondria, triggering mitochondria-mediated apoptosis in response to light and greatly reducing the mitochondrial ATP concentration.PDT produces ROS, which oxidizes the phospholipids in the mitochondrial membrane by involving oxygen molecules in energy or electron transport.Depolarization and a decrease in the mitochondrial membrane potential cause mitochondrial expansion, which damages the outer mitochondrial membrane [83].Both PTT and PDT were capable of producing ROS when exposed to light irradiation.The application and breakdown of Cu-based NMs increased the amount of Cu ions in tumor cells, decreasing the amount of GSH and promoting ROS accumulation [84].Mitochondrial damage prompted by high ROS leads to Cyt c releasing into the cytoplasm and upregulation of the Bax gene.The increase in Cu ions also resulted in an elevated production of Bax, Cyt C, and caspase3/9, which, when combined with PTT/PDT, led tumor cells to undergo an apoptotic cascade reaction [79].Mithun et al. synthesized biotin-Cu@AuNP and discovered that when laser irradiated, NPs accumulated considerably in the mitochondria of A549 cells.Moreover, the caspase3/7 activity notably increased, suggesting that NPs triggered apoptosis via the caspase-activation pathway [85].In summary, Cu can induce the production of pro-apoptotic factors and ROS through direct or indirect effects on mitochondria, PTT/PDT can cause ROS damage to mitochondria through phototoxicity, and Cu-based NMs synergizes PTT/PDT-induced apoptosis, increasing the death of tumor cells and resulting in anti-tumor therapy.The illustration of Cu-based NMs synergizes PTT/PDT-induced apoptosis is shown in Figure 3.
By binding to apoptosis-activating caspase 9, the Cu induced an increase in Bax leve causing the release of Cyt c and mitochondrial apoptosis-inducing factor 1 (Apaf-1) from the mitochondria into the cytoplasm, where it activated caspase 3 to cause apoptosis.Sim ultaneously, ROS caused DNA breakage in PC12 cells and activated the caspase cascad response [79].Additionally, Cu-induced ROS increased lipid peroxidation and decrease GSH levels, making cells more vulnerable to oxidative stress-induced harm [80,81].Hos seini et al. reported that Cu 2+ interacted with respiratory complexes (I, II, and IV) and pro moted ROS generation in mouse hepatocytes in vitro [82].Mitochondria is also an im portant target for PAs/PSs.Hilf showed that PSs enter tumor cells and are rapidly take up by mitochondria, triggering mitochondria-mediated apoptosis in response to light an greatly reducing the mitochondrial ATP concentration.PDT produces ROS, which ox dizes the phospholipids in the mitochondrial membrane by involving oxygen molecule in energy or electron transport.Depolarization and a decrease in the mitochondrial mem brane potential cause mitochondrial expansion, which damages the outer mitochondria membrane [83].Both PTT and PDT were capable of producing ROS when exposed to ligh irradiation.The application and breakdown of Cu-based NMs increased the amount o Cu ions in tumor cells, decreasing the amount of GSH and promoting ROS accumulatio [84]

Cu-Based NMs Synergizes PTT/PDT-Induced Autophagy
Many studies have demonstrated that Cu ions can trigger and inhibit autophagic processes via different pathways [86,87].Polishchuk et al. analyzed gene enrichment using the ATP7B mouse model and found that an insufficient level of ATP7B restricted Cu efflux and increased intracellular Cu concentration [88].The autophagy marker MAP1LC3 (also known as LC3) level was greatly increased, indicating that autophagy was activated while also interfering with the inactivation of the mammalian target of rapamycin (mTOR)dependent signaling on the lysosomal membrane [89,90].Autophagy is still promoted by mTOR deactivation because it can stimulate dephosphorylation and transcription factor EB (TFEB) translocation to the nucleus [88,91].The unc-51-like autophagy activating kinase (ULK)1/2 is a known downstream target of mTOR.Donita C et al. discovered that ULK1 kinase activity was increased in Cu-sufficient cells and decreased in Cu-deficient cells, indicating that the Cu level directly affected ULK1/2 activity [92,93].The Cu transporter 1 (Ctr1) deletion or ULK1 mutation inhibited Cu binding, which increased intracellular Cu levels and boosted ULK1 kinase activity, producing an autophagy complex to trigger autophagy [94].Furthermore, Ctr1 deletion, considered pertinent to the high Cucontent-induced autophagy, inhibited tumor development in the Kirsten rat sarcoma virus G12D-driven lung cancers [95].Qin et al. reported that copper sulfate (CuSO 4 ) increased mitochondrial ROS formation (mtROS) and activated autophagy in RAW264.7 cells via the protein kinase B/adenosine monophosphate-activated protein kinase/mTOR pathway.Additionally, rapamycin-stimulated autophagy decreased apoptosis, whereas autophagyrelated 5 knockdown to promote autophagy increased CuSO 4 -induced apoptosis [96].Li et al. reported HYF127c/Cu, a completely novel Cu combination.The transcriptome sequencing revealed that some autophagy genes, including the MAP1LC3B gene, were considerably upregulated in HYF127c/Cu-treated HeLa cells and that HYF127c/Cu was reported to activate autophagy in HeLa cells [97].Studies have shown that ROS produced by PDT can activate and result in a long-lasting increase in autophagy.Whether PTT/PDTinduced survival autophagy is primarily protective or death autophagic is still undetermined [98].Numerous studies have suggested that thermal stress induces pro-survival autophagy in tumor cells, resulting in treatment resistance in PTT/PDT [99].Cu-based NMs are capable of carrying autophagy inhibitors.Cu ions can improve anti-tumor efficacy by inducing pre-survival autophagy, inhibiting the heat-induced pro-survival autophagy produced by PTT/PDT and generating ROS for CDT treatment through a Fenton-like reaction, combining PTT/PDT and CDT to achieve the improvement of antitumor efficacy [29].For example, Wen et al. synthesized Cu palladium alloy tetrapod NPs (TNP-1) with good photothermal characteristics that also activated autophagy due to an increased Cu 2+ release.In the 4T1 and MCF7/multidrug-resistant breast cancer models, the combination of TNP-1 with the autophagy inhibitors CQ or 3-MA significantly enhanced the anticancer efficacy of TNP-1-mediated PTT [100].Although the exact mechanism of PTT/PDT-induced autophagy is yet to be known, Cu-based NMs may trigger autophagy in PTT/PDT to induce antitumor effects.The illustration of Cu-based NMs synergizes PTT/PDT-induced autophagy is shown in Figure 4.
(mTOR)-dependent signaling on the lysosomal membrane [89,90].Autophagy is still pro moted by mTOR deactivation because it can stimulate dephosphorylation and transcrip tion factor EB (TFEB) translocation to the nucleus [88,91].The unc-51-like autophagy act vating kinase (ULK)1/2 is a known downstream target of mTOR.Donita C et al. discov ered that ULK1 kinase activity was increased in Cu-sufficient cells and decreased in Cu deficient cells, indicating that the Cu level directly affected ULK1/2 activity [92,93].Th Cu transporter 1 (Ctr1) deletion or ULK1 mutation inhibited Cu binding, which increase intracellular Cu levels and boosted ULK1 kinase activity, producing an autophagy com plex to trigger autophagy [94].Furthermore, Ctr1 deletion, considered pertinent to th high Cu-content-induced autophagy, inhibited tumor development in the Kirsten rat sar coma virus G12D-driven lung cancers [95].Qin et al. reported that copper sulfate (CuSO increased mitochondrial ROS formation (mtROS) and activated autophagy in RAW264.cells via the protein kinase B/adenosine monophosphate-activated protein kinase/mTO pathway.Additionally, rapamycin-stimulated autophagy decreased apoptosis, wherea autophagy-related 5 knockdown to promote autophagy increased CuSO4-induced apop tosis [96].Li et al. reported HYF127c/Cu, a completely novel Cu combination.The tran scriptome sequencing revealed that some autophagy genes, including the MAP1LC3 gene, were considerably upregulated in HYF127c/Cu-treated HeLa cells and tha HYF127c/Cu was reported to activate autophagy in HeLa cells [97].Studies have show that ROS produced by PDT can activate and result in a long-lasting increase in autophagy Whether PTT/PDT-induced survival autophagy is primarily protective or death au tophagic is still undetermined [98].Numerous studies have suggested that thermal stres induces pro-survival autophagy in tumor cells, resulting in treatment resistance i PTT/PDT [99].Cu-based NMs are capable of carrying autophagy inhibitors.Cu ions ca improve anti-tumor efficacy by inducing pre-survival autophagy, inhibiting the heat-in duced pro-survival autophagy produced by PTT/PDT and generating ROS for CDT trea ment through a Fenton-like reaction, combining PTT/PDT and CDT to achieve the im provement of antitumor efficacy [29].For example, Wen et al. synthesized Cu palladium alloy tetrapod NPs (TNP-1) with good photothermal characteristics that also activated au tophagy due to an increased Cu 2+ release.In the 4T1 and MCF7/multidrug-resistant breas cancer models, the combination of TNP-1 with the autophagy inhibitors CQ or 3-MA sig nificantly enhanced the anticancer efficacy of TNP-1-mediated PTT [100].Although th exact mechanism of PTT/PDT-induced autophagy is yet to be known, Cu-based NMs ma trigger autophagy in PTT/PDT to induce antitumor effects.The illustration of Cu-base NMs synergizes PTT/PDT-induced autophagy is shown in Figure 4.

Cu-Based NMs Synergizes PTT/PDT-Induced Pyroptosis
Cu 2+ can be reduced by GSH to Cu 1+ in the TME that overexpress GSH.Cu 1+/2+ and PTT/PDT may together activate the generation of ROS under UV irradiation, resulting in cancer cell pyroptosis [101].In a study, CuSO 4 markedly expedited cellular pyroptosis via the NLRP3/caspase-1/GSDMD pathway.Furthermore, the pyroptosis-associated genes IL-1 and NLRP3 exhibited an increased expression in hepatocytes, which promotes inflammation and causes the extravasation of immune cells [102][103][104].The inflammasome caspase-1 is essential for pyroptosis, a highly inflammatory form of programmed cell death [105,106].In cancer cells, increasing the intracellular ROS levels activates the nucleotide-binding oligomerized structural domain-like receptor protein 3 (NLRP3) inflammasome and causes pyroptosis [107].Wang et al. s synthesized GOx@Cu MOFs produced excessive •OH radicals, utilizing the internal biological cascade reaction of glucose oxidase (GOx) and the Fenton-like reaction of Cu ions, resulting in tumor cell pyroptosis [108].In the work by Yin et al., it was discovered that higher Cu 2+ concentrations and longer exposure times decreased the protein content of the cAMP/PKA/CREB pathway, as well as the potential of the mitochondrial membrane and the amount of GSH-Px (Glutathione peroxidase) in MN9D cells.Concurrently, enhanced ROS generation, as well as the expression of Nrf2, NQO1, HSP-70, and other proteins, resulted in the creation of inflammasome and mediated the overexpression of GSDMD proteins, resulting in pyroptosis in MN9D cells [109].PTT/PDT therapy is a promising strategy for non-invasively inducing pyroptosis in cancer cells [105].PDT effectively initiates pyroptosis via inflammasome activation and the production of ROS; PTT can accelerate this process, making it the ideal complement to enhance photoimmunotherapy s efficacy [110].Tang et al. designed the aggregation-induced dimeric photosensitizer D1 (Figure 5b).This compound targets tumor cell membranes and, when used with PTT/PDT, proves highly effective in ROS production and, subsequently, pyroptosis induction [102].The surge in intracellular ROS contributes to the pyroptosis instigated by Cu ions.At present, the specific research mechanism of Cu-induced cell pyroptosis in PTT/PDT is still unclear, but Cu and PTT/PDT operate through distinct mechanisms to induce tumor cell death.Their combined anti-tumor approach should offer synergistic effects, exemplified by the principle "1 + 1 > 2." The illustration of Cu-based NMs synergizes PTT/PDT-induced pyroptosis is shown in Figure 5a.
Cu 2+ can be reduced by GSH to Cu 1+ in the TME that overexpress GSH.Cu 1+/2+ an PTT/PDT may together activate the generation of ROS under UV irradiation, resulting i cancer cell pyroptosis [101].In a study, CuSO4 markedly expedited cellular pyroptosis vi the NLRP3/caspase-1/GSDMD pathway.Furthermore, the pyroptosis-associated gene IL-1 and NLRP3 exhibited an increased expression in hepatocytes, which promotes in flammation and causes the extravasation of immune cells [102][103][104].The inflammasom caspase-1 is essential for pyroptosis, a highly inflammatory form of programmed ce death [105,106].In cancer cells, increasing the intracellular ROS levels activates the nucle otide-binding oligomerized structural domain-like receptor protein 3 (NLRP3) inflam masome and causes pyroptosis [107].Wang et al.′s synthesized GOx@Cu MOFs produce excessive •OH radicals, utilizing the internal biological cascade reaction of glucose oxidas (GOx) and the Fenton-like reaction of Cu ions, resulting in tumor cell pyroptosis [108].I the work by Yin et al., it was discovered that higher Cu 2+ concentrations and longer expo sure times decreased the protein content of the cAMP/PKA/CREB pathway, as well as th potential of the mitochondrial membrane and the amount of GSH-Px (Glutathione perox idase) in MN9D cells.Concurrently, enhanced ROS generation, as well as the expressio of Nrf2, NQO1, HSP-70, and other proteins, resulted in the creation of inflammasome an mediated the overexpression of GSDMD proteins, resulting in pyroptosis in MN9D cell [109].PTT/PDT therapy is a promising strategy for non-invasively inducing pyroptosis i cancer cells [105].PDT effectively initiates pyroptosis via inflammasome activation an the production of ROS; PTT can accelerate this process, making it the ideal complemen to enhance photoimmunotherapy′s efficacy [110].Tang et al. designed the aggregation induced dimeric photosensitizer D1 (Figure 5b).This compound targets tumor cell mem branes and, when used with PTT/PDT, proves highly effective in ROS production and subsequently, pyroptosis induction [102]

Cu-Based NMs Synergizes PTT/PDT-Induced Cuproptosis
The phenomenon of cuproptosis emerges when an excessive concentration of Cu ac cumulates within a cell.This buildup promotes the enrichment of lipoylated dihy drolipoamide S-acetyltransferase (DLAT).Subsequently, DLAT disruption affects th

Cu-Based NMs Synergizes PTT/PDT-Induced Cuproptosis
The phenomenon of cuproptosis emerges when an excessive concentration of Cu accumulates within a cell.This buildup promotes the enrichment of lipoylated dihydrolipoamide S-acetyltransferase (DLAT).Subsequently, DLAT disruption affects the tricarboxylic acid cycle (TCA) during mitochondrial respiration, inducing proteotoxic stress and leading to cell death [111].Xie et al. developed a Cu 2+ -doped nanohybrid gel integrated with a PTT/PDT/CDT therapeutic combination [112].The NIR-responsive capability of Cu 2+ harmonizes with Au in the gel, optimizing PTT/PDT tumor treatment [113].Simultaneously, Cu 2+ mitigates the challenge of tumor hypoxia, enhancing the therapeutic efficacy of PDT.This effect also results in the degradation of Cu ions due to the excessive accumulation of Cu 2+ in TMEs with a low pH.Additionally, Cu 2+ interacts with GSH, transforming into Cu 1+ and GSH oxidized (GSSG), enabling CDT to produce more harmful •OH radicals [112,114].Furthermore, in vitro data revealed that the mortality rate of activated cancer cells was greater than 81.4%, while tumor suppression in vivo was as high as 85.7%, demonstrating cuproptosis-enhanced PDT/PTT/CDT against malignant tumors.Zhu et al. synthesized Cu-doped polymetallic oxalate nanoclusters (Cu-POM), enhancing their uptake in cells via MPTT, leading to intracellular Cu-POM accumulation.Excessive Cu interrupts the initiation of the TCA cycle and promotes intracellular peroxide formation, culminating in cuproptosis [115].Therefore, cuproptosis offers a novel therapeutic approach for tumor treatment by regulating intracellular Cu levels in tumor cells.Moreover, Cu plays a pivotal role in PTT/PDT antitumor strategies.The illustration of Cu-based NMs synergizes PTT/PDT-induced cuproptosis is shown in Figure 6.
tricarboxylic acid cycle (TCA) during mitochondrial respiration, inducing proteotoxi stress and leading to cell death [111].Xie et al. developed a Cu 2+ -doped nanohybrid ge integrated with a PTT/PDT/CDT therapeutic combination [112].The NIR-responsive ca pability of Cu 2+ harmonizes with Au in the gel, optimizing PTT/PDT tumor treatmen [113].Simultaneously, Cu 2+ mitigates the challenge of tumor hypoxia, enhancing the ther apeutic efficacy of PDT.This effect also results in the degradation of Cu ions due to th excessive accumulation of Cu 2+ in TMEs with a low pH.Additionally, Cu 2+ interacts with GSH, transforming into Cu 1+ and GSH oxidized (GSSG), enabling CDT to produce mor harmful •OH radicals [112,114].Furthermore, in vitro data revealed that the mortality rat of activated cancer cells was greater than 81.4%, while tumor suppression in vivo was a high as 85.7%, demonstrating cuproptosis-enhanced PDT/PTT/CDT against malignant tu mors.Zhu et al. synthesized Cu-doped polymetallic oxalate nanoclusters (Cu-POM), en hancing their uptake in cells via MPTT, leading to intracellular Cu-POM accumulation Excessive Cu interrupts the initiation of the TCA cycle and promotes intracellular perox ide formation, culminating in cuproptosis [115].Therefore, cuproptosis offers a novel ther apeutic approach for tumor treatment by regulating intracellular Cu levels in tumor cells Moreover, Cu plays a pivotal role in PTT/PDT antitumor strategies.The illustration of Cu based NMs synergizes PTT/PDT-induced cuproptosis is shown in Figure 6.In conclusion, Cu and PTT/PDT may both be employed in various ways in anti-tumo therapy (Figure 7).In treating tumors, they are complimentary to one another.Compared to a single therapy method, this synergistic approach can produce be er anti-tumor ben efits.In conclusion, Cu and PTT/PDT may both be employed in various ways in anti-tumor therapy (Figure 7).In treating tumors, they are complimentary to one another.Compared to a single therapy method, this synergistic approach can produce better anti-tumor benefits.
stress and leading to cell death [111].Xie et al. developed a Cu 2+ -doped nanohybrid ge integrated with a PTT/PDT/CDT therapeutic combination [112].The NIR-responsive ca pability of Cu 2+ harmonizes with Au in the gel, optimizing PTT/PDT tumor treatmen [113].Simultaneously, Cu 2+ mitigates the challenge of tumor hypoxia, enhancing the ther apeutic efficacy of PDT.This effect also results in the degradation of Cu ions due to the excessive accumulation of Cu 2+ in TMEs with a low pH.Additionally, Cu 2+ interacts with GSH, transforming into Cu 1+ and GSH oxidized (GSSG), enabling CDT to produce more harmful •OH radicals [112,114].Furthermore, in vitro data revealed that the mortality rate of activated cancer cells was greater than 81.4%, while tumor suppression in vivo was a high as 85.7%, demonstrating cuproptosis-enhanced PDT/PTT/CDT against malignant tu mors.Zhu et al. synthesized Cu-doped polymetallic oxalate nanoclusters (Cu-POM), en hancing their uptake in cells via MPTT, leading to intracellular Cu-POM accumulation Excessive Cu interrupts the initiation of the TCA cycle and promotes intracellular perox ide formation, culminating in cuproptosis [115].Therefore, cuproptosis offers a novel ther apeutic approach for tumor treatment by regulating intracellular Cu levels in tumor cells Moreover, Cu plays a pivotal role in PTT/PDT antitumor strategies.The illustration of Cu based NMs synergizes PTT/PDT-induced cuproptosis is shown in Figure 6.In conclusion, Cu and PTT/PDT may both be employed in various ways in anti-tumo therapy (Figure 7).In treating tumors, they are complimentary to one another.Compared to a single therapy method, this synergistic approach can produce be er anti-tumor ben efits.[116] 2022 Elsevier, (c) [117] 2020 Elsevier and (d) [118] 2023 American Chemical Society are the schematic representation of the role of different Cu-based NMs in anti-tumor therapy.

Application of Different Kinds of Cu-Based NMs in PTT/PDT 4.1. Application of Copper Oxides in PTT and PDT
Over the past few decades, various Cu-based NMs have emerged as alternative means to amplify the effects of PTT/PDT [119].Studies indicate that CuO-NPs can hinder pancreatic tumor growth, notably by targeting tumor stem cells [120].These NPs can induce mitochondrial dysfunction, attributed to the ability of Cu 2+ to generate ROS through the Fenton-like reaction and to enhance PCE through electronic transitions between C-2p and Cu-3d [121].Ma et al. developed CuO@CNSs-DOX (DOX: Doxorubicin) nanoplatforms, elevating the PCE from 6.7% to 10.14%.These platforms achieve anti-tumor effects through the drug action of DOX and the generation of •OH radicals by Cu 2+ (Figure 8b) [122,123].The same group synthesized multifunctional MoS 2 -CuO@BSA/R837 (MCBR, BSA: Bovine Serum Albumin, R837: Imiquimod, a toll-like receptor 7 (TLR7) agonist) nanoflowers.Under 808 nm laser exposure, these nanoflowers exhibited a PCE of 24.6% and a marked increase in •OH production.Additionally, the inclusion of R837 increased the expression of calreticulin (CRT), triggering immunogenic cell death (ICD), effectively neutralizing primary tumors, and inhibiting metastatic tumors (Figure 8a) [124].Taking advantage of Cu 2 O's high refractive index above 600 nm, Yu et al. produced core/shell structured Cu@Cu 2 O@polymer NPs.Under a 660 nm laser exposure and an equivalent Cu concentration, compared to Cu@polymer NPs, the temperature increase for Cu@Cu 2 O@polymer NPs was about 4.2 • C.This resulted in a temperature rise of at least 23 • C in adjacent tissues, translating to a 7-fold surge in the IC 50 , relative to prior research [125,126].These findings underscore that Cu@Cu 2 O@polymer NPs possess sufficient phototoxicity to exterminate tumor cells.Furthermore, the increased concentration of endogenous H 2 O 2 produced by the LPS endotoxin accelerated the release of Cu ions and ROS, facilitating the eradication of cancer cells (Figure 8c) [33,125].

Application of Cu x S y in PTT and PDT
Semiconductor NMs Cu x S y have gained attraction in catalysis and sensing sectors due to their optical and electrical properties.Owing to their affordability, minimal cytotoxicity, and high photostability, Cu x S y NPs hold promise as Pas [127,128].Tian et al. synthesized hydrophilic, plate-like Cu 9 S 5 nanocrystals.With a 980 nm laser, the PCE of Cu 9 S 5 reached 25.7%.Interestingly, under identical conditions, this PCE surpassed that of the synthesized Au nanocrystals, which was approximately 23.7%.These nanocrystals eradicated tumor cells in vivo swiftly (in less than 10 min), establishing Cu 9 S 5 nanocrystals as efficient PAs.When hormonal mice underwent subsequent radiation following injection, notable necrosis and shrinkage of tumor cells in the mass region were observed [129,130].Addressing the challenge that non-targeted ultrasmall metallic NPs face in securing prolonged half-life and satisfactory tumor-site aggregation as PTT agents, Li et al. synthesized CuS NPs and modified them with cetuximab (Ab) to yield CuS-Ab NPs.On exposure to a 1064 nm laser for 10 min, temperatures increase rapidly from 23 • C to 58 • C and are consistently held at 58 • C. Additionally, Ab modification rendered CuS-Ab NPs superior in tumor-targeting and antiangiogenic capacities, ensuring effective tumor-site accumulation without concomitant damage to other tissues and organs [131].Cationic polymers, such as PEI, can achieve drug delivery by intensifying the electrostatic adsorption of cations on the cell membrane [132].Studies have shown that NMs modified with PEI and PSs can be instrumental in the PDT of bladder tumors [133].Based on this, Mu et al. prepared HRP@CPC@HA NPs (HRP: horseradish peroxidase, CPC: CuS-PEI-Ce6, HA: hyaluronic acid).These particles incorporated HRP within a hollow CuS nanocage [134].Subjected to 808 nm laser radiation, their PCE was around 34.91%.Concurrently, the surface-bound Ce6 was activated, leading to a large amount of 1 O 2 generation, the levels of which increased alongside temperature [133].Additionally, HRP catalyzed the breakdown of intracellular H 2 O 2 to oxygen, addressing the oxygen deficit in tumor cells and facilitating simultaneous multimodal tumor eradication in severely hypoxic TME [135].Due to the increased concentration of endogenous H 2 S in tumor cells, Wu et al. synthesize monolocking NPs (MLNPs) [136].When illuminated with 808 nm radiation, these MLNPs combined with H 2 S and produced ultra-small CuS nanodots.This interaction elevated tumor temperatures, triggering apoptosis in tumor cells.Mel is released from the NPs which causes the COX-2 enzyme to become inactive, amplifying the PTT action and inhibiting inflammation induced by PTT damage.Notably, these NPs were excreted renally (Figure 8e,f) [137].The development of nanomedicine has witnessed diverse manifestations of Cu x S y .Hollow structured Cu x S y has particularly piqued interest in oncologic therapy, attributed to its drug-loading ability and high PCE and PAI capability [138,139], yet many extant synthesis methods for CuS-based NMs are intricate, time-consuming, and require special reaction equipment.Consequently, there is an evident need for facile, cost-effective methodologies that employ gentle reaction conditions to fabricate Cu x S y NMs.

Application of Copper Selenides and Copper Telluride in PTT and PDT
Copper selenides, notably Cu 2-X Se NMs, present promising applications in imaging and PTT for cancer treatment, attributed to their biocompatibility, excellent NIR light absorption, and increased PCE (Figure 8g) [140,141].
Du et al. synthesized CuSe/NC-DOX-DNA NPs (NC: nitrogen-doped carbon) via an environmentally friendly and simple method.Under 808 nm laser exposure, these NPs displayed a PCE of 32.9%, surpassing that of Au nanorods which had a PCE of 22.0% [142].Moreover, the synergistic action of photocatalysis combined with the Fentonlike reaction of CuSe/NC markedly augmented ROS generation [143].Concurrently, the encapsulated DOX functioned as a chemotherapeutic agent, realizing a threefold enhanced PTT/CDT/PCT tumor treatment method [144].It is noteworthy that Cu 2-X Se has been identified as an effective PA [141].In another study, He et al. developed ICG@Cu 2-X Se-ZIF-8 (ICG: Indocyanine Green, ZIF-8: a metal-organic framework).After 808 nm laser exposure, this compound exhibited a PCE of 15.5%.In the TME, ZIF-8 breaks down and releases Cu 1+ and Cu 2+ , which leads to a Fenton-like reaction, controls the amount of GSH, and produces ROS (Figure 8d) [145].Furthermore, selenium has the ability to regulate selenoprotein, allowing it to prevent the production of osteoclasts and tumor cells, resulting in the synergistic PTT/CDT prevention of malignant bone metastasis [146,147].Moreover, CuSe is an ideal PS and has been demonstrated to biodegrade.Selenium is released during this process and has been shown to lower the risk of liver cancer, lung cancer, and prostate cancer [148,149].Pun et al. fabricated COF-CuSe NMs (COF: covalent-organic framework); under 808 nm laser irradiation, the PCE was 26.34% after injection in mice.A large quantity of 1 O 2 was generated under both 650 nm and 808 nm illumination.Consequently, almost all of the HeLa cells died under laser irradiation.The PTT and PDT anti-tumor treatment strategies showed a synergistic potential (Figure 8h) [150].At present, CuSe NMs have been poorly investigated for PTT/PDT and the development of multifunctional CuSe NM is still required to improve anti-tumor therapy for PTT/PDT.Similar to CuSe, CuTe is considered a novel PAs candidate.Li et al. created CuTe NPs with a plasma peak at 900 nm.Under 830 nm laser irradiation, the mortality of 3T3 embryonic fibroblasts increased [151].However, it was discovered that some cells were already dead before laser irradiation.It has been demonstrated that CuTe NPs are cytotoxic and PAs.Under 1064 nm laser irradiation, bio Cu 2−X Te nanosheets synthesized by Li et al. had a PCE of 48.6%.When Cu 1+ and Te are released, the nanosheets can produce •OH and inhibit the GPx and TrxR enzymes for CDT, significantly inhibiting the proliferation of MCF-7 cells [152].Shen et al. synthesized CM CTNPs@OVA NMs (CM: melanoma B16-OVA membrane, OVA: ovalbumin) with solid CuTe NPs.Under laser irradiation, there was a significant increase in temperature and production of ROS.In addition, B16-OVA cells produced an abundance of ATP and HMGB-1, which effectively stimulated the immune system and enhanced the anti-tumor treatment [153].CuTe NPs anti-tumor therapy research in PTT/PDT has received less attention and requires further development and investigation.[124] 2021 Elsevier, (b) [123] 2011 American Chemical Society, (c) [33] 2018 American Chemical Society, (d) [145] 2022 John Wiley and Sons, (e,f) [137] 2021 John Wiley and Sons, (g) [140] 2013 John Wiley and Sons, and (h) [150] 2022 American Chemical Society.[152].Shen et al. synthesized CM CTNPs@OVA NMs (CM: melanoma B16-OVA membrane, OVA: ovalbumin) with solid CuTe NPs.Under laser irradiation, there was a significant increase in temperature and production of ROS.In addition, B16-OVA cells produced an abundance of ATP and HMGB-1, which effectively stimulated the immune system and enhanced the anti-tumor treatment [153].CuTe NPs anti-tumor therapy research in PTT/PDT has received less a ention and requires further development and investigation.

Application of Cu-Based Nanocomposites in PTT and PDT
Most treatments consisting of a single therapy have a negligible impact on tumor treatment.However, a synergistic combination of multiple therapeutic modalities can improve the efficacy of treating malignant tumors [154].The same applies to NMs.In cancer treatment, simple Cu-based NMs such as CuS may still be lacking.Therefore, it is necessary to load various materials, drugs, or fluorescents onto Cu-based NMs to create a composite material capable of fluorescence, tumor targeting, and therapy in a single NM.
As a result of the discovery of cuproptosis, Cu-based NMs may induce tumor cell death by modulating the concentration of Cu in tumor cells, offering a novel anti-tumor therapeutic modality [111].Pan et al. produced GOx@[Cu( )] NPs.Under 808 nm laser irradiation, NPs entering tumor cells produced H2O2 and •OH.In the meantime, under the influence of GSH, GOx hydrolyzed and consumed glucose, generating a large amount of H2O2 and OH that produced a ROS-adding effect [155].Due to the depletion of glucose and GSH, the NPs bind to lipoylated mitochondrial enzymes, resulting in the aggregation of lipoylated DLAT, which induces cuproptosis and effectively inhibits tumor growth  [123] 2011 American Chemical Society, (c) [33] 2018 American Chemical Society, (d) [145] 2022 John Wiley and Sons, (e,f) [137] 2021 John Wiley and Sons, (g) [140] 2013 John Wiley and Sons, and (h) [150] 2022 American Chemical Society.− to produce •OH [164].The fluorescence intensity of the HPF was used to detect the ROS concentration; it was discovered that the fluorescence intensity of the HPF increased rapidly in the presence of NSCuCy NPs under 660 nm irradiation.At pH 5.5, the emission intensity of HPF increased nearly 350-fold after 10 min of irradiation, demonstrating a higher •OH production efficiency than that at pH 7.4 (180-fold) and targeting accumulation in tumor tissues to achieve complete tumor ablation [165].Kang et al. developed Au@MSN-Cu/PEG/DSF NPs (Au@MSN: mesoporous silica-coated Au nanorods, DSF: disulfiram).The PCE under 808 nm laser irradiation is 56.32%.With an increase in temperature, the Cu-doped SiO 2 framework begins to biodegrade.During the conversion of Cu 2+ to Cu 1+ , releasing DSF can chelate with Cu 2+ to produce highly cytotoxic bis (diethyldithiocarbamate) Cu (CuET) [166,167].Cu 1+ binds additionally to mitochondrial protein aggregates during the TCA cycle, inducing cuproptosis in tumor cells [111].Photothermal therapy s synergistic effect resulted in an 80.1% tumor inhibition rate, effectively killing tumor cells and inhibiting tumor growth [111,168].
Overall, multifunctional nanocomposite materials that integrate imaging, diagnosis, and therapy have shown significant improvements in tumor treatment compared to single-material therapy.These NMs combine PTT/PDT with drug delivery systems, immunotherapy, and chemotherapy, reducing normal medication dosage and adverse reactions while achieving effective anti-tumor treatment in the short term (Figure 9).However, long-term experimental data on NMs are still needed to verify their long-term toxicity, biosafety, and therapeutic effects to ensure they meet desired goals.Further investigations are required for a comprehensive understanding.

Application of Cu-MOF in PTT and PDT
Metal-organic frameworks (MOFs) are a novel type of NMs that combine metal ions or clusters with multisite organic ligands.These MOFs have shown great potential in antitumor therapy due to their inherent biodegradability, high porosity, structural diversity, and high drug-loading capacity [170][171][172][173].
For instance, Wu et al. synthesized ultrathin Cu-TCPP MOF nanosheets containing both Cu 1+ and Cu 2+ which enabled imaging, photothermal conversion, and anti-tumor therapy [36,174].Under 808 nm laser irradiation, the nanosheets showed a PCE of 36.8% and a significant amount of 1 O 2 was generated in tumor cells.Cu 2+ has unpaired 3d electrons and paramagnetic also allowed the nanosheets to be used for T1-weighted MRI.This multifunctional NMOF design holds promise for tumor PTT [59].Tang et al. designed two metal-organic materials, MOF-1 and MOF-2, based on this premise.MOF-1 is an aluminum (AL)-MOF that does not contain Cu 2+ , whereas MOF-2 is an AL-Cu mixedmetal MOF[CuL-[AlOH] 2 n with Cu 2+ at its core.Comparing the two MOFs under 650 nm laser irradiation revealed that MOF-2 has a porous physical structure, which makes the Cu 2+ + in MOF-2 significantly adsorb intracellular GSH and results in a decrease in GSH and an increase in ROS concentration, which enhances the efficacy of PDT [175].Similar to the chemotherapeutic drug camptothecin (CPT), MOF-2 was able to eradicate tumor cells in an in vivo experiment.The results of a simultaneous examination of major organ tissue slices revealed little toxicity in vivo [176,177].This MOF structure, which simultaneously decreases intracellular GSH levels and increases intracellular ROS levels, may provide not only a new strategy for intracellular GSH adsorption but also a new method for enhancing PDT.
Due to the higher cavity structure of emerging Cu-NMOFs, they can be loaded with more PSs or PAs to improve PCE and increase the diffusion range of ROS.Their porous structure also allows for chemotherapeutic drug delivery and synergistic effects with other therapies to enhance anti-tumor efficacy.However, the synthesis process of MOF PSs or PAs can be complex and might exhibit batch-to-batch variations, hindering large-scale NMOF preparation.Moreover, the presence of various metal ions necessitates further investigation into the long-term safety, biocompatibility, pharmacokinetics, and immunoreactivity of NMOFs in mammals through systematic animal studies.

Conclusions and Future Perspectives
One of the most significant challenges in cancer treatment today is achieving precise targeting.PTT and PDT have demonstrated promising results for early surface cancers.Moreover, combining various PAs and PSs has significantly improved the therapeutic efficacy of PTT/PDT for deep tumors.Recently, Cu-based NMs have emerged as promising candidates due to their excellent NIR absorption, paramagnetic characteristics that can be used for tumor imaging, ability to be degraded in the TME, and the capacity to generate ROS via the Fenton-like reaction.In addition, Cu-based NMs can be used to inhibit tumor growth by carrying various types of drugs (DFS, DOX, or R837), not only for tumor-sitetargeted delivery, but also for synergistic effects of various therapeutic modalities, such as PTT, PDT, CDT, and immunotherapy.
However, some important considerations need to be addressed.Firstly, there is no consensus on the best way to synthesize Cu-based NMs; the preparation and use of Cubased NMs may generate wastewater and exhaust fumes, which could cause potential threats to environmental safety that should be extensively investigated and controlled.The most environmentally friendly way of synthesizing Cu-based NMs on a wide scale for clinical use is still being researched in order to minimize their detrimental impact on humans and the natural world.Cu-based NMs have not yet been used in actual clinical practice because the long-term biosafety, pharmacokinetics, and immunoreactivity of Cubased NMs has not been adequately explored and only the short-term safety of Cu-based NMs has been validated in tumor-bearing mice.Therefore, their safety and toxicity must be completely established through long-term rigorous mammalian experimental testing.
In conclusion, the application of Cu-based NMs in preclinical therapies has considerable advantages.Combining Cu-based NMs with PTT/PDT shows great promise for anti-tumor therapy.Our most critical mission is to promote human health to realize accurate, safe, and effective cancer treatments.Cu-based NMs produced for clinical application should be more precise and careful, but we still look forward to the development of more Cu-based NMs or other nanocomposites which have undergone comprehensive biosafety testing and have proven to be reliable.

Figure 1 .
Figure 1.Functions of Cu and therapeutic involvement.CDT: chemodynamic therapy.2. Principles and Drawbacks of PTT/PDT in Antitumor Treatment and the Benefits of Cu-Based NMs 2.1.Principles and Drawbacks of PTT/PDT in Antitumor Treatment PTT can be divided into traditional PTT (≥45 • C) and mild PTT (MPTT, 42-45• C)[39].It is an antitumor therapy where PAs convert laser light energy into thermal energy under NIR irradiation, raising the temperature of the surrounding region and inducing apoptosis or necrosis in the tumor cells[40].In PDT, PSs generate ROS in the presence of oxygen molecules upon or following photoirradiating of the tumor tissue, resulting in oxidative damage-induced tumor cell death[41].PDT is often classified as type I or type II, where type I produce O 2 − , hydroxyl radicals (•OH), and H 2 O 2 , and type II produce 1 O 2[42,43].Although PTT and PDT may both cause tumor cell death by phototoxicity and producing ROS, they still have notable drawbacks in actual use[44].First, the practical usage of PTT/PDT is generally constrained by PAs/PSs because of the low tissue penetration of light, which leads to poor tumor cell death, tumor resistance to therapy, and possible excruciating pain during treatment[45,46].Additionally, with the rise in local temperature around the tumor, adjacent normal tissues and cells may also suffer damage.Finally, ROS production is dependent on the oxygen present around the tissue, but the low oxygen content in the tumor microenvironment (TME) severely restricts the effectiveness of PDT [47].To resolve these problems, NMs are incorporated into PTT and PDT; Cu-based NMs can act as carriers or modified PAs or PSs to fix the limitations of conventional PAs or PSs[43,48].For example, Zhang et al. produced biodegradable cancer cell membrane-coated mesoporous Cu/manganese (Mn) silicate nanospheres (m@CMSNs) to target the aggregation of homozygous cancer cells via adhesion molecules on the surface of cancer cell membranes[49,50].Under 635 nm laser irradiation, m@CMSNs were targeted to aggregate 2.85-fold more than CMSNs at the tumor site and degraded by glutathione (GSH) in the TME.Cu 1+ functions as a very effective PSs, producing huge levels of 1 O 2 at the tumor site, killing tumor cells and reducing tumor cell viability to 20%[51].Thus, the deficiencies of conventional PAs/PSs, which are especially addressed in detail in the next section, can be significantly improved by Cu-based NMs.
Synergizes PTT/PDT-Induced Tumor Cell Death 3.1.Cu-Based NMs Synergizes PTT/PDT-Induced Tumor Cell Necrosis PTT/PDT can eliminate tumor cells via multiple pathways.When exposed to exogenous light, PSs/PAs attached to tumor cell membranes and lysosomes may directly lead to tumor cell necrosis[70].Cu has a photothermal ability and a high NIR absorption capacity; therefore, Cu-containing PSs/PAs localized in the tumor cell membrane interact with the surrounding oxygen molecules during NIR irradiation, leading to an elevated energy level and the formation of 1 O 2 through oxygen energy transfer.Then, et al. synthesized LPS-CuS NPs (LPS: lipopolysaccharide), induced tumor ablation via laser irradiation, and found increased interleukin (IL)-6, IL-12p40 and tumor necrosis factor-α mRNA levels in tumor-draining lymph nodes.The mRNA levels of T-helper-1 cell transcription factors interferon and T-bet expressed in T cells were also increased, which increased tumor antigen-specific immune response by promoting DC activation [77].Jiang et al. synthesized AuNBP@CuS (NBP: nanobipyramid) NMs with a core/shell design and found that NIR irradiation increased extracellular ATP, calreticulin (CRT), and high mobility group box-1 (HMGB-1) levels.Both of them are immunological signals that, within a specified concentration range, can effectively increase the expression of CD80 and CD86 signals in DCs and promote DCs maturation [78].Studies have shown that PTT and Cu-based NMs worked synergistically to create DAMPs from dying cells and activate immunological responses.The illustration of Cu-based NMs synergizes PTT/PDT-induced tumor cell necrosis is shown in Figure 2.
et al. synthesized LPS-CuS NP (LPS: lipopolysaccharide), induced tumor ablation via laser irradiation, and found in creased interleukin (IL)-6, IL-12p40 and tumor necrosis factor-α mRNA levels in tumo draining lymph nodes.The mRNA levels of T-helper-1 cell transcription factors interfero and T-bet expressed in T cells were also increased, which increased tumor antigen-specifi immune response by promoting DC activation [77].Jiang et al. synthesized AuNBP@Cu (NBP: nanobipyramid) NMs with a core/shell design and found that NIR irradiation in creased extracellular ATP, calreticulin (CRT), and high mobility group box-1 (HMGB-1 levels.Both of them are immunological signals that, within a specified concentratio range, can effectively increase the expression of CD80 and CD86 signals in DCs and pro mote DCs maturation [78].Studies have shown that PTT and Cu-based NMs worked syn ergistically to create DAMPs from dying cells and activate immunological responses.Th illustration of Cu-based NMs synergizes PTT/PDT-induced tumor cell necrosis is show in Figure 2.

Figure 2 .
Figure 2. Cu-Based NMs synergizes PTT/PDT-induced tumor cell necrosis.Cu-containing PSs/PA a ached to tumor cells lead to tumor cell necrosis, stimulating the immune system by DAMPs.

Figure 2 .
Figure 2. Cu-Based NMs synergizes PTT/PDT-induced tumor cell necrosis.Cu-containing PSs/PAs attached to tumor cells lead to tumor cell necrosis, stimulating the immune system by DAMPs.
. Mitochondrial damage prompted by high ROS leads to Cyt c releasing into the cyto plasm and upregulation of the Bax gene.The increase in Cu ions also resulted in an ele vated production of Bax, Cyt C, and caspase3/9, which, when combined with PTT/PDT led tumor cells to undergo an apoptotic cascade reaction [79].Mithun et al. synthesize biotin-Cu@AuNP and discovered that when laser irradiated, NPs accumulated consider ably in the mitochondria of A549 cells.Moreover, the caspase3/7 activity notably in creased, suggesting that NPs triggered apoptosis via the caspase-activation pathway [85 In summary, Cu can induce the production of pro-apoptotic factors and ROS through d rect or indirect effects on mitochondria, PTT/PDT can cause ROS damage to mitochondri through phototoxicity, and Cu-based NMs synergizes PTT/PDT-induced apoptosis, in creasing the death of tumor cells and resulting in anti-tumor therapy.The illustration o Cu-based NMs synergizes PTT/PDT-induced apoptosis is shown in Figure 3.

Figure 4 .
Figure 4. Cu-Based NMs synergizes PTT/PDT-induced autophagy.Autophagy is activated and th mTOR-dependent signaling is also interfered by the increased intracellular Cu concentration.

Figure 4 .
Figure 4. Cu-Based NMs synergizes PTT/PDT-induced autophagy.Autophagy is activated and the mTOR-dependent signaling is also interfered by the increased intracellular Cu concentration.
. The surge in intracellular ROS contributes t the pyroptosis instigated by Cu ions.At present, the specific research mechanism of Cu induced cell pyroptosis in PTT/PDT is still unclear, but Cu and PTT/PDT operate throug distinct mechanisms to induce tumor cell death.Their combined anti-tumor approac should offer synergistic effects, exemplified by the principle "1 + 1 > 2." The illustration o Cu-based NMs synergizes PTT/PDT-induced pyroptosis is shown in Figure 5a.

Figure 7 .
Figure 7. Anti-tumor therapeutic process of PTT/PDT with different kinds of Cu-based NMs.(a) Schematic representation of the synthesis process of a Cu-based NMs.And (b)[116] 2022 Elsevier, (c)[117] 2020 Elsevier and (d)[118] 2023 American Chemical Society are the schematic representation of the role of different Cu-based NMs in anti-tumor therapy.
Similar to CuSe, CuTe is considered a novel PAs candidate.Li et al. created CuTe NPs with a plasma peak at 900 nm.Under 830 nm laser irradiation, the mortality of 3T3 embryonic fibroblasts increased [151].However, it was discovered that some cells were already dead before laser irradiation.It has been demonstrated that CuTe NPs are cytotoxic and PAs.Under 1064 nm laser irradiation, bio Cu2−XTe nanosheets synthesized by Li et al. had a PCE of 48.6%.When Cu 1+ and Te are released, the nanosheets can produce •OH and inhibit the GPx and TrxR enzymes for CDT, significantly inhibiting the proliferation of MCF-7 cells

4. 4 .
Application of Cu-Based Nanocomposites in PTT and PDTMost treatments consisting of a single therapy have a negligible impact on tumor treatment.However, a synergistic combination of multiple therapeutic modalities can improve the efficacy of treating malignant tumors[154].The same applies to NMs.In cancer treatment, simple Cu-based NMs such as CuS may still be lacking.Therefore, it is necessary to load various materials, drugs, or fluorescents onto Cu-based NMs to create a composite material capable of fluorescence, tumor targeting, and therapy in a single NM.As a result of the discovery of cuproptosis, Cu-based NMs may induce tumor cell death by modulating the concentration of Cu in tumor cells, offering a novel anti-tumor therapeutic modality[111].Pan et al. produced GOx@[Cu(tz)] NPs.Under 808 nm laser irradiation, NPs entering tumor cells produced H 2 O 2 and •OH.In the meantime, under the influence of GSH, GOx hydrolyzed and consumed glucose, generating a large amount of H 2 O 2 and OH that produced a ROS-adding effect[155].Due to the depletion of glucose and GSH, the NPs bind to lipoylated mitochondrial enzymes, resulting in the aggregation of lipoylated DLAT, which induces cuproptosis and effectively inhibits tumor growth (92.4% inhibition rate)[156].The synthesis and development of NMs that combine multiple antitumor therapeutic modalities is urgently needed.Xia et al. synthesized metal-organic skeleton nanosheets Cu-TCPP(Al)-Pt-FA (TCPP: Tetrakis (4-carboxyphenyl) porphyrin, FA: folic acid) with surface modification by platinum NPs (Pt NPs) and FA in order to solve the problem of poor oxygenation in tumor tissues.Compared to 25.2% tumor cell survival in vitro without laser irradiation, laser irradiation at 638 nm reduced tumor cell survival to 20.7%.Since Cu 2+ can react with GSH via a Fenton-like reaction, it depletes intracellular GSH and increases ROS levels [157].In the meantime, Pt NPs have catalysis activity comparable to a catalase-like reaction that can continuously convert intracellular H 2 O 2 to O 2 in order to alleviate hypoxic TME and enhance the therapeutic effect of PDT [158].ROS concentration was increased synergistically by these two modalities, which stimulated antigen-presenting cells to activate systemic anti-tumor immune responses and increased the infiltration of cytotoxic T lymphocytes (CTLs) at the tumor site for synergistic immune anti-tumor therapy [159,160].Xu et al. constructed d-Cu-LDH/ICG NPs (LDH: lactatedehydrogenase) in order to alleviate the tumor hypoxia problem and avoid the poor therapeutic effect caused by tumor hypoxia during PDT treatment [161].Under 808 nm laser irradiation, the PCE was 88.7% and the production of 1 O 2 increased as the temperature rose.In the meantime, the rising temperature led to the dissolution of the NMs and the reduction in Cu 2+ to Cu 1+ by GSH, both of which can consume excessive H 2 O 2 and generate OH in tumor cells via a Fenton-like reaction, resulting in CDT and modulation of the TME [162].In the meantime, it was demonstrated that hormonal mice had significantly less tumor growth.Hematoxylin and eosin (H&E) staining of major organs revealed no obvious inflammation or damage and demonstrated that the effective anti-tumor agent exhibited no significant systemic toxicity.Yang et al. created NSCuCy NPs containing Cu 1+ as the core [163].Cu 1+ undergoes a Fenton-like reaction with O 2

Figure 9 .
Figure 9. Tumor regression in Cu-based NMs applied in tumor-bearing mice and effects on vital organs.Scale bar: 100 µm.Ref. [169] 2021 John Wiley and Sons.