Development of a Ready-to-Use-Type RNA Vaccine Carrier Based on an Intracellular Environment-Responsive Lipid-like Material with Immune-Activating Vitamin E Scaffolds

Because of its efficient and robust gene transfer capability, messenger RNA (mRNA) has become a promising tool in various research fields. The lipid nanoparticle (LNP) is considered to be a fundamental technology for an mRNA delivery system and has been used extensively for the development of RNA vaccines against SARS-CoV-2. We recently developed ssPalm, an environmentally responsive lipid-like material, as a component of LNP for mRNA delivery. In this study, a self-degradable unit (phenyl ester) that confers high transfection activity and an immune stimulating unit (vitamin E scaffold) for high immune activation were combined to design a material, namely, ssPalmE-Phe-P4C2, for vaccine use. To design a simple and user-friendly form of an RNA vaccine based on this material, a freeze-drying-based preparation method for producing a ready-to-use-type LNP (LNP(RtoU)) was used to prepare the LNPssPalmE-Phe. The optimization of the preparation method and the lipid composition of the LNPssPalmE-Phe(RtoU) revealed that dioleoyl-sn-glycero phosphatidylethanolamine (DOPE) was a suitable helper lipid for achieving a high vaccination activity of the LNPssPalmE-Phe(RtoU). Other findings indicated that to maintain particle properties and vaccination activity, a 40% cholesterol content was necessary. A single administration of the LNPssPalmE-Phe(RtoU) that contained mRNA-encoding Ovalbumin (mOVA-LNPssPalmE-Phe(RtoU)) demonstrated a significant suppression of tumor progression in a tumor-bearing mouse OVA-expressing cell line (E.G7-OVA). In summary, the LNPssPalmE-Phe(RtoU) is an easy-to-handle drug delivery system (DDS) for delivering mRNA antigens in immunotherapy.


Introduction
Nucleic-acid-based vaccines are based on the use of plasmid DNA (pDNA) or messenger RNA (mRNA) as a source of antigens.Upon the administration of such a vaccine, these genetic materials need to be delivered into the nucleus/cytoplasm, their site of action, where they will be transcribed/translated into antigen proteins.Since the mRNA can function in the cytoplasm and nuclear localization is not required, the mRNA can provide efficient and robust gene transfer, even in non-dividing cells [1][2][3].On the other hand, since mRNA is highly susceptible to enzymatic degradation in extracellular fluid, the development of suitable delivery technology is a prerequisite for its clinical application [3,4].One of the more promising carriers for RNA delivery is lipid nanoparticles (LNPs), which contain an ionizable lipid as the main component.In 2018, ONPATTRO ® (Alnylam Pharmaceutical, Cambridge, MA, USA), an LNP-based therapeutic using small interfering RNA (siRNA), became the first ever approved RNA interference (RNAi) therapeutic for the treatment of hereditary transthyretin-mediated amyloidosis [5][6][7][8][9].Moreover, the approval of RNA vaccines (mRNA-LNPs) against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) [10][11][12][13][14] further demonstrated the versatility of the LNP as an mRNA carrier.
We have been developing LNPs that are composed of a series of ionizable lipids, referred to as SS-cleavable and pH-activated lipid-like materials (ssPalm).The ssPalm(s) respond to the difference in extracellular and intracellular environments based on their dual-sensing motifs: tertiary amines and a disulfide bond.After cellular uptake, the neutrally charged LNPs ssPalm are sorted into endosomes.When arriving in the endosome, the LNPs are exposed to the acidic environment in the endosomal compartment (pH 6.5-5.5) and develop positive charges on their surface through the protonation of tertiary amines.The positively charged LNPs then cause endosomal membrane disruption/destabilization, which results in the endosomal escape of their cargo.Following this endosomal escape event, the disulfide bond in LNPs ssPalm will be cleaved by glutathione in the reductive environment of the cytoplasm.This then triggers the decapsulation of the mRNA cargoes, releasing them into the cytoplasm [15][16][17].
As additional modifications of the ionizable lipids, we have focused on the hydrophobic scaffold and the linker between the amine and the scaffolds.Derivatives of ssPalms can be developed by changing their hydrophobic scaffolds.Changing the hydrophobic scaffolds from fatty acids such as myristic acid (ssPalmM) to retinoic acid (vitamin A; ssPalmA) allowed the intracellular trafficking of the nucleic acid cargo to be controlled [15].Types of ssPalm with vitamin E scaffolds (ssPalmE) have become a focus of vaccine development since they are equipped with both gene expression activity and immune activation properties [16].The vitamin E scaffolds were assumed to be suitable for cancer vaccines because they can efficiently activate cell-mediated immunity via the type I interferon pathway [18].In addition to the modification of hydrophobic scaffolds, the incorporation of a self-degradable phenyl ester moiety can be used to improve the gene transfer activity of the mRNA-LNP [15,17].The cleavage of disulfide bonds by reducing agents in the cytoplasm elevates the concentration of hydrophobic thiols in the particle.Within those limited intraparticle spaces, the concentrated thiols attack the phenyl ester and trigger a nucleophilic substitution reaction.This self-degradation reaction is irreversible, leading to the more efficient collapse of particles, which further enhances the release of the nucleic acid cargo [15,17].In this study, a phenyl ester moiety (for achieving high transfection activity) and vitamin E scaffolds (for promoting immune activation) were combined to design ssPalmE-Phe-P4C2 for RNA vaccine development.
LNPs are generally prepared using the alcohol dilution method.In this method, the lipids and nucleic acids spontaneously form a complex due to the electrostatic interactions between the positively charged amine groups and the negatively charged nucleic acids, as well as the hydrophobic interactions between the hydrophobic moieties of lipids [19].However, we recently reported that the post-encapsulation of mRNA into a pre-formed LNP is also applicable for use in preparing an mRNA-LNP [20].Therefore, it is possible to encapsulate a desired mRNA into an mRNA-LNP by using pre-formed ready-to-use (RtoU) types of LNPs.Since the LNP(RtoU) can be used simply by rehydration and incubation, only a typical temperature-controlling device (i.e., block incubator, water bath, or thermal cycler) is needed for use.The development of an LNP(RtoU) using ssPalmE-Phe-P4C2 (LNP ssPalmE-Phe (RtoU)) would produce a convenient form of an mRNA vaccine that offers handling practicality and vaccination efficacy.In this study, we investigated the potency of ssPalmE-Phe-P4C2 as an RNA vaccine and optimized the conditions needed for the preparation of the LNP ssPalmE-Phe (RtoU).The ability to induce cytotoxic T lymphocyte (CTL) activation and antitumor activity against an E.G7-OVA tumor model was also investigated.

Animals
C57BL/6J mice (female, 6 weeks) were purchased from Japan SLC, Inc. (Shizuoka, Japan).Protocols for the animal experiments were reviewed and approved by the Chiba University Animal Care Committee following the "Guide for Care and Use of Laboratory Animals".

Preparation of LNPs ssPalmE-Phe (RtoU)
The lipid composition was ssPalmE-Phe-P4C2/DOPE (or DOPC)/Chol = 52.5/7.5/40 with additional DMG-PEG 2000 (3 mol% of total lipid) [17].The amount of total lipid was 100 nmol, encapsulating 0.5 µg of mRNA (lipid/mRNA: 200 nmol/µg).The lipid mixtures were dissolved in 90% t-butanol to a concentration of 8.0 mM.The lipid mixtures in t-butanol and buffer solution, containing 40 mM MES buffer (pH 5.0, salt-free) with a 744 mg/mL solution of sucrose (in equal volume), were set to their respective syringes in the NanoAssem-blr™ Benchtop device (Precision Nanosystems, Vancouver, Canada) with the settings of flow rate = 1.0 mL/min, flow rate ratio (buffer:lipid) = 3:1, and total volume = 0.52 mL.An equal volume of 320 mg/mL of sucrose solution (#30404-45, nacalai tesque, Kyoto, Japan) was added (final concentration of sucrose in the LNP solution = 320 mg/mL).The LNP solution was frozen in liquid nitrogen and transferred to the freeze-dryer (FDU-2110, EYELA, Tokyo, Japan).The drying sequence of the freeze-dryer was set to 9 segments: −40 ), and 30 • C (3 h).The freeze-dried LNPs were recovered, and their physical appearances were inspected for visible cracks, dents, or collapses in front of a black background [21].The dry LNPs were reconstituted with a water solution containing 0.5 µg of either mLuc or mOVA (0.0025 µg/µL).The solution was mixed well, followed by heating at 95 • C for 5 min, then allowed to cool to room temperature (RT) for approximately 10 min before use in the experiments/analysis.

Characterization of mRNA-LNPs
The particle size, polydispersity index (PdI), and zeta-potential of the LNPs were measured via dynamic light scattering (Zetasizer Nano ZS, Malvern Panalytical, Malvern, UK).The recovery ratio and encapsulation efficiency of the mRNA were evaluated with RiboGreen ® assay.Solutions containing Quant-iT™ RiboGreen ® RNA reagent (#R11491, Invitrogen, Waltham, MA, USA) with or without 10% (v/v) TritonX-100 (#168-11805, FU-JIFILM Wako Pure Chemical Corporation, Tokyo, Japan), in respective 1:8 ratios were prepared in D-PBS (−): Triton [+] (contained both RiboGreen ® and TritonX-100) and Triton [−] (no TritonX-100, only RiboGreen ® ).The mRNA-LNPs, corresponding to 50 ng of mRNA in 50 µL of D-PBS (−), were prepared twice and each mixed with an equal volume of the Triton [+] and Triton [−] solution in a 96-well black microplate.A calibration curve was prepared through sequential dilution from 0 to 2000 ng/mL of mRNA.The plate was incubated for 5 min with shaking at 500 rpm in a shaking incubator.The fluorescence intensities were analyzed with a plate reader (Infinite M200 PRO, TECAN, Männedorf, Switzerland) set with emission and excitation waves of 484 nm and 535 nm, respectively.The recovery ratio was calculated from the total mRNA (quantified by Triton [+] addition) and mRNA concentration input (standard curve).The encapsulation efficiency was calculated as the ratio of the total mRNA concentration (quantified by Triton [+]) to the non-encapsulated mRNA concentration (quantified by Triton [−] addition).

Evaluation of In Vitro Gene Expression Efficiency
BMDCs (8 × 10 5 cells/mL) and RAW 264.7 cells (2 × 10 5 cells/mL) seeded in a 12-well plate were transfected with mLuc-LNPs in D-PBS (−) at a dose of 0.8 µg of mRNA.In the case of RAW 264.7 cells, the transfection was demonstrated after pre-incubation for 24 h.After transfection, the BMDCs and RAW 264.7 cells were incubated at 37 • C with 5% CO 2 for 5 h.The BMDCs and RAW 264.7 cell suspensions were collected, washed with D-PBS (−), and then solubilized with Reporter Lysis Buffer (#E397A, Promega, Madison, WI, USA).The cell lysates were added with a Nano-Glo ® Luciferase Assay System (#E1501, Promega), and the luminescence intensities were quantified with a luminometer (GloMax ® 20/20 Luminometer, Promega, Madison, WI, USA).The protein amount in the cell lysates was determined using the Bicinchoninic acid (BCA) Protein Assay kit (#T9300A, Takara Bio Inc., Kusatsu, Shiga, Japan) according to the manufacturer's instructions.Luciferase activity was represented as a relative light unit (RLU/mg protein), calculated by dividing the luminescence intensity by the protein amount [16].

Evaluation of In Vivo Gene Expression Efficiency (IVIS Imaging)
The mLuc-LNPs in D-PBS (−) were administered subcutaneously (back of neck) to C57BL/6J mice at a dose of 1.0 µg of mRNA under anesthetized conditions.The neck region of mice was shaved in advance.Six hours later, D-Luciferin potassium (#126-05116, FUJI-FILM Wako Pure Chemical Corporation, Tokyo, Japan) in D-PBS (−) (3 mg/200 µL/mouse) was administered intraperitoneally.After 30 min had passed, the luminescence intensities were measured with an In Vivo Imaging System (IVIS ® Lumina II, Caliper Life Sciences, Waltham, MA, USA).
Both an in vitro and in vivo quantifications of gene expression were performed to evaluate the effect of the phenyl ester group on transfection efficiency.An in vitro luciferase assay was performed using bone-marrow-derived dendritic cells (BMDCs) (Figure 2b) and RAW 264.7 cells of a murine macrophage/monocyte cell line (Figure 2c).Luciferase activity was evaluated 6 h after the transfection.The mLuc-LNPs ssPalmE-Phe exhibited significantly higher luciferase activity in the BMDCs compared to the mLuc-LNPs ssPalmE .The gene expression of the mLuc-LNPs ssPalmE-Phe in RAW 264.7 cells was also significantly higher than that of the LNPs ssPalmE (Figure 2c).A similar trend was also found in the in vivo gene expression of mLuc-LNPs on the skin at the back of the neck at a dose of 1.0 µg of mRNA (Figure 2d,e).Both an in vitro and in vivo quantifications of gene expression were performed to evaluate the effect of the phenyl ester group on transfection efficiency.An in vitro luciferase assay was performed using bone-marrow-derived dendritic cells (BMDCs) (Figure 2b) and RAW 264.7 cells of a murine macrophage/monocyte cell line (Figure 2c).Luciferase activity was evaluated 6 h after the transfection.The mLuc-LNPsssPalmE-Phe exhibited significantly higher luciferase activity in the BMDCs compared to the mLuc-LNPsssPalmE.The gene expression of the mLuc-LNPsssPalmE-Phe in RAW 264.7 cells was also significantly higher than that of the LNPsssPalmE (Figure 2c).A similar trend was also found in the in vivo  The ability to activate cell-mediated immunity for both the LNPsssPalmE-Phe and the LNPsssPalmE, with either DOPE or DOPC as helper lipids, was evaluated with a CTL assay against the model antigen ovalbumin (OVA).As shown in Figure 3, the mOVA-LNPsssPalmE- The ability to activate cell-mediated immunity for both the LNPs ssPalmE-Phe and the LNPs ssPalmE , with either DOPE or DOPC as helper lipids, was evaluated with a CTL assay against the model antigen ovalbumin (OVA).As shown in Figure 3, the mOVA-LNPs ssPalmE-Phe had an overall higher CTL activity than the mOVA-LNPs ssPalmE , with either helper lipid being used.The use of DOPE as a helper lipid in the mOVA-LNPs ssPalmE-Phe provided the highest CTL activity (Figure 3).Collectively, the higher gene expression activity and vaccination activity of the LNPs ssPalmE-Phe indicated that the insertion of a phenyl ester group improved the function of the ionizable lipid with a vitamin E scaffold.
Pharmaceutics 2023, 15, x 9 of 20 Phe had an overall higher CTL activity than the mOVA-LNPsssPalmE, with either helper lipid being used.The use of DOPE as a helper lipid in the mOVA-LNPsssPalmE-Phe provided the highest CTL activity (Figure 3).Collectively, the higher gene expression activity and vaccination activity of the LNPsssPalmE-Phe indicated that the insertion of a phenyl ester group improved the function of the ionizable lipid with a vitamin E scaffold.

Ready-to-Use-Type RNA Vaccine Development
The preparation method for the lyophilized ready-to-use LNP formulation (LNPssPalm(RtoU)) was then applied to the LNPsssPalmE-Phe to further improve their usability.To apply the preparation method, the lipid composition was adjusted to the previously reported LNPsssPalm(RtoU) (ssPalm/DOPC/Chol = 52.5/7.5/40(% of total lipid)) [17,20].We initially applied both the default RtoU composition (ssPalmE-Phe-P4C2/helper lipid/cholesterol = 52.5/7.5/40) and the microfluidic-mixer-type LNPsssPalmE-Phe (ssPalmE-Phe-P4C2/helper lipid/cholesterol = 60/30/10), with a fixed DMG-PEG2000 ratio of 3 mol% of total lipid.Two types of helper lipids, DOPE and DOPC, were used in the lipid formulation.A stock solution of the lipids prepared in 90% t-butanol was prepared.The mixture of the lipids was then mixed with a 40 mM MES (pH 5.0) buffer with a NanoAssemblr device to prepare empty LNPs.An equal volume of 320 mg/mL of sucrose was then added into the empty LNPsssPalmE-Phe.The LNP solution was frozen in liquid nitrogen and then transferred to the freeze-drying equipment.The recovered lyophilized empty LNPsssPalmE-Phe(RtoU) can be reconstituted with the intended mRNA solution in an aqueous medium, followed by heating at 95°C for 5 min.After cooling, the LNPs were neutralized with an equal volume of PBS and used for the following experiments/analysis (Figure 1b).

Ready-to-Use-Type RNA Vaccine Development
The preparation method for the lyophilized ready-to-use LNP formulation (LNP ssPalm (RtoU)) was then applied to the LNPs ssPalmE-Phe to further improve their usability.To apply the preparation method, the lipid composition was adjusted to the previously reported LNPs ssPalm (RtoU) (ssPalm/DOPC/Chol = 52.5/7.5/40(% of total lipid)) [17,20].We initially applied both the default RtoU composition (ssPalmE-Phe-P4C2/helper lipid/cholesterol = 52.5/7.5/40) and the microfluidic-mixer-type LNPs ssPalmE-Phe (ssPalmE-Phe-P4C2/helper lipid/cholesterol = 60/30/10), with a fixed DMG-PEG 2000 ratio of 3 mol% of total lipid.Two types of helper lipids, DOPE and DOPC, were used in the lipid formulation.A stock solution of the lipids prepared in 90% tbutanol was prepared.The mixture of the lipids was then mixed with a 40 mM MES (pH 5.0) buffer with a NanoAssemblr device to prepare empty LNPs.An equal volume of 320 mg/mL of sucrose was then added into the empty LNPs ssPalmE-Phe .The LNP solution was frozen in liquid nitrogen and then transferred to the freeze-drying equipment.The recovered lyophilized empty LNPs ssPalmE-Phe (RtoU) can be reconstituted with the intended mRNA solution in an aqueous medium, followed by heating at 95 • C for 5 min.After cooling, the LNPs were neutralized with an equal volume of PBS and used for the following experiments/analysis (Figure 1b).

Optimization of Buffer pH and Cryoprotectant Concentration
Concerning the preparation of the LNPs ssPalmE-Phe (RtoU), an acidic buffer pH is important since the cationic charge is accompanied by the protonation of the ionizable lipid, which is plausibly a crucial driving force for the post-encapsulation of the negatively charged mRNA.In parallel, a cryoprotectant is needed to protect the LNPs from damage during the freeze-drying process [20].The regimen for preparing the LNPs ssPalmE-Phe (RtoU) was optimized by changing the pH of the 20 mM MES buffer (pH 5.00-6.00)and sucrose concentrations (80-320 mg/mL).As a first screening, the freeze-dried appearance and physicochemical properties of the mOVA-LNPs ssPalmE-Phe (RtoU) after rehydration are also shown in Table 2.The appearance of the freeze-dried material was labeled as 'good', 'dent', 'crack', and 'collapse' (Figure S2) based on the literature [21,22].The LNPs ssPalmE-Phe (RtoU) that were labeled as 'good' or 'dent' were considered to have an acceptable appearance [22].The lyophilized LNPs ssPalmE-Phe (RtoU) that showed an acceptable appearance and properties were further evaluated for their capabilities to induce immune activity: LNPs ssPalmE-Phe (RtoU) with a buffer of pH 5.25 had 80 mg/mL of sucrose, pH 5.25-160 mg/mL of sucrose, pH 5.50-160 mg/mL of sucrose, and pH 5.00-320 mg/mL of sucrose.As a result, the LNPs ssPalmE-Phe (RtoU) prepared with a buffer pH of 5.00 and 320 mg/mL of sucrose exhibited the highest CTL activity among the other groups and was comparable with those with a buffer pH of 5.50 and a sucrose concentration of 160 mg/mL (Figure 5).The lyophilized appearance of the LNPs ssPalmE-Phe (RtoU) with a buffer with a pH of 5.00 and a sucrose concentration of 320 mg/mL (dent) appeared to be not as good as those with a buffer pH of 5.50 and a sucrose concentration of 160 mg/mL (good).However, the particle size and PdI of the LNPs ssPalmE-Phe (RtoU) with a buffer pH of 5.00 and a sucrose concentration of 320 mg/mL were better (Table 2).This indicated that the 'dent' appearance might have negligible effects on the properties of the of LNPs ssPalmE-Phe (RtoU) after hydration with mRNA.Based on these results, we concluded that the optimal buffer and sucrose concentrations for preparing the LNPs ssPalmE-Phe (RtoU) were 20 mM of MES buffer (pH 5.00) and 320 mg/mL of sucrose, respectively.

Lipid Composition Screening
To investigate the issue of whether the ssPalm-Phe-P4C2/DOPE/Chol = 52.5/7.5/4composition was optimal for LNPsssPalmE-Phe(RtoU), a screening of the lipid compositio was further conducted using a total of 24 lipid compositions with various cholesterol an DOPE ratios (Table S2) through CTL assays.All formulations produced mRNA LNPsssPalmE-Phe(RtoU) with sizes ranging from 160 to 230 nm, PdIs ranging from 0.150 t 0.240, acceptable appearances (dent), encapsulation efficiencies ≥90%, and recovery rate ≥80%.The mRNA-LNPsssPalmE-Phe(RtoU) with the ssPalmE-Phe-P4C2/DOPE/Chol 52.5/7.5/40formulation were used as a control in each evaluation.Within the same chole terol ratio, the CTL activities were maintained or tended to increase with a reduction i the DOPE ratio (Figures 6a and S3a-c).Thus, a lipid composition with a lower DOPE den sity was evaluated.

Lipid Composition Screening
To investigate the issue of whether the ssPalm-Phe-P4C2/DOPE/Chol = 52.5/7.5/40composition was optimal for LNPs ssPalmE-Phe (RtoU), a screening of the lipid composition was further conducted using a total of 24 lipid compositions with various cholesterol and DOPE ratios (Table S2) through CTL assays.All formulations produced mRNA-LNPs ssPalmE-Phe (RtoU) with sizes ranging from 160 to 230 nm, PdIs ranging from 0.150 to 0.240, acceptable appearances (dent), encapsulation efficiencies ≥90%, and recovery rates ≥80%.The mRNA-LNPs ssPalmE-Phe (RtoU) with the ssPalmE-Phe-P4C2/DOPE/Chol = 52.5/7.5/40formulation were used as a control in each evaluation.Within the same cholesterol ratio, the CTL activities were maintained or tended to increase with a reduction in the DOPE ratio (Figures 6a and  S3a-c).Thus, a lipid composition with a lower DOPE density was evaluated.
In the LNPs ssPalmE-Phe (RtoU) with 40% Chol, the optimal range of DOPE was narrow (5-10%): the CTL activity increased when the DOPE ratio was 0-5% but then slightly decreased when the density of DOPE reached 7.5% (Figure 6b).Meanwhile, for the LNPs ssPalmE-Phe (RtoU) with 50% Chol, the CTL activity increased when the DOPE ratio was 0-7.5% and then sharply decreased with a DOPE ratio above 10% (Figure 6c).Similarly, the optimal DOPE ratio was also narrow (5-10% DOPE).(d) CTL activity of LNPsssPalmE-Phe(RtoU) with a DOPE ratio of 5-10% and 40 or 50% Chol.The lipid composition of ssPalmE-Phe-P4C2/DOPE/Chol = 52.5/7.5/40 was still found to be the favorable formulation for LNPsssPalmE-Phe(RtoU).The scatter graph represents individual values; the bar graph represents the mean with SD (n = 3); white bar: control group; N.S.: not significant; * p < 0.05; ** p < 0.01 (one-way ANOVA followed by Bonferroni testing against the control group).There were no significant differences found in all groups (against control group) in Figure 6a.
Finally, the CTL activities of the LNPsssPalmE-Phe(RtoU) with 5-10% DOPE and 40 or 50% Chol were compared.The LNPsssPalmE-Phe(RtoU) with 50% Chol and a DOPE ratio of 7.5% showed comparable CTL activity with those with 40% Chol and a DOPE ratio of 7.5% (control) (Figure 6d).However, the CTL activity of the LNPsssPalmE-Phe(RtoU) with 50% Chol was more sensitive to the content of DOPE (Figure 6d).This drastic loss in CTL activity depending on the DOPE density is not desirable when considering batch-to-batch reproducibility.We therefore concluded that the initial formulation of ssPalmE-Phe-P4C2/DOPE/Chol = 52.5/7.5/40 was the most favorable lipid composition for LNPsssPalmE-Phe(RtoU) from the viewpoint of CTL activity and physicochemical properties: with a particle size of 158.9 ± 0.4 nm, a PdI of 0.19 ± 7.8, a dented freeze-dried appearance, a 100 ± 2.3% encapsulation efficiency, and a 102.1 ± 7.1% recovery rate.

Therapeutic Anti-Tumor Response against E.G7-OVA Tumor Model
To determine the capability of the LNPssPalmE-Phe(RtoU) to function as an mRNA-based vaccine carrier, its therapeutic anti-tumor response against an E.G7-OVA (murine-thymus-lymphoma-expressing ovalbumin) tumor model was evaluated.A subcutaneous administration (a single dose of 0.5 µg of mRNA) significantly suppressed tumor progression.Moreover, the survival analysis indicated that the immunized mice completely survived until 30 days after the tumor inoculation, when the endpoint was set at a 1000 mm 3 the bar graph represents the mean with SD (n = 3); white bar: control group; N.S.: not significant; * p < 0.05; ** p < 0.01 (one-way ANOVA followed by Bonferroni testing against the control group).There were no significant differences found in all groups (against control group) in Figure 6a.
Finally, the CTL activities of the LNPs ssPalmE-Phe (RtoU) with 5-10% DOPE and 40 or 50% Chol were compared.The LNPs ssPalmE-Phe (RtoU) with 50% Chol and a DOPE ratio of 7.5% showed comparable CTL activity with those with 40% Chol and a DOPE ratio of 7.5% (control) (Figure 6d).However, the CTL activity of the LNPs ssPalmE-Phe (RtoU) with 50% Chol was more sensitive to the content of DOPE (Figure 6d).This drastic loss in CTL activity depending on the DOPE density is not desirable when considering batch-to-batch reproducibility.We therefore concluded that the initial formulation of ssPalmE-Phe-P4C2/DOPE/Chol = 52.5/7.5/40 was the most favorable lipid composition for LNPs ssPalmE-Phe (RtoU) from the viewpoint of CTL activity and physicochemical properties: with a particle size of 158.9 ± 0.4 nm, a PdI of 0.19 ± 7.8, a dented freeze-dried appearance, a 100 ± 2.3% encapsulation efficiency, and a 102.1 ± 7.1% recovery rate.

Therapeutic Anti-Tumor Response against E.G7-OVA Tumor Model
To determine the capability of the LNP ssPalmE-Phe (RtoU) to function as an mRNA-based vaccine carrier, its therapeutic anti-tumor response against an E.G7-OVA (murine-thymuslymphoma-expressing ovalbumin) tumor model was evaluated.A subcutaneous administra-tion (a single dose of 0.5 µg of mRNA) significantly suppressed tumor progression.Moreover, the survival analysis indicated that the immunized mice completely survived until 30 days after the tumor inoculation, when the endpoint was set at a 1000 mm 3 tumor size (Figure 7).Collectively, LNPs ssPalmE-Phe (RtoU) can be a potent mRNA-based vaccine carrier.

Discussion
The ionizable lipid ssPalmE-Phe-P4C2 was designed so as to combine two important functions for an RNA vaccine: a vitamin E scaffold for immune activation and a phenyl ester for self-degradability, respectively.Regarding Vitamin E, it has been used as a key component in AS03, a squalene-based oil-in-water emulsion adjuvant that is used in vac-

Discussion
The ionizable lipid ssPalmE-Phe-P4C2 was designed so as to combine two important functions for an RNA vaccine: a vitamin E scaffold for immune activation and a phenyl ester for self-degradability, respectively.Regarding Vitamin E, it has been used as a key component in AS03, a squalene-based oil-in-water emulsion adjuvant that is used in vaccines against the avian influenza virus [23][24][25].It has been reported that the vitamin E in the AS03-adjuvanted vaccine modulates the production of cytokines and chemokines such as CCL2, CCL3, IL-6, CSF3, and CXCL1 and promotes antigen-loading monocytes as well as the recruitment of granulocytes [26].The ssPalm with vitamin E scaffolds (ssPalmE) was initially developed to deliver nucleic acids to the liver; the delivery of siRNA by LNPs ssPalmE demonstrated gene knockdown efficiency and extensive liver accumulation compared to other ssPalms with myristic acid or retinoic acid [15].The molecular tuning of the ssPalmE through attaching tertiary amines to a piperizine ring resulted in the formation of the second-generation ssPalmE-P4C2, which demonstrated an efficient endosomal escape activity, which contributed to the improved gene knockdown efficiency [15,17].However, LNPs prepared with ssPalmE derivatives (with piperazine as the tertiary amine structure) triggered severe inflammatory responses that involved the production of pro-inflammatory cytokines (IL6, TNFα), the intracellular DNA-sensor-related cytokine (IL-1β), and interferon (IFNβ) when they were combined with plasmid DNA.From these observations, we hypothesized that the LNPs formed with the ssPalmE derivative would be applicable for use as an RNA vaccine [16].It was revealed that the mRNA LNPs ssPalmE acted as both a nucleic acid (mRNA) carrier and as an immune adjuvant by inducing the proliferation of antigen-specific CD8+ T cells and their differentiation into effector and memory cells to activate cellular immunity [18].However, the mechanism concerning the ssPalmE-triggered immune activation is not clear.
Studies have demonstrated the capability of vaccines and an adjuvant to induce immunogenicity by stimulating the damage-associated molecular patterns (DAMPs) pathway [27,28].Cellular stressors or cell death signaling can trigger the release of DAMPs that are then recognized by other cells via pattern recognition receptors (PRRs).This interaction will result in immunogenic reactions, such as the upregulation of the chemokine/cytokines involved in immune responses.Renowned vaccine adjuvants such as Alum, AS03, and HP-β-cyclodextrin are known to induce the release of DAMP signals, which stimulates and enhances the immunogenicity of vaccines [27].Our previous study also reported the detection of dsDNA at the injection site (skin) upon LNP ssPalmE administration.This finding suggests that the adjuvant effect of LNPs ssPalmE-Phe (RtoU) could also be dependent on this DAMP pathway [18].Further investigation is required into the cells and molecules involved in this response.It is noteworthy that the use of a simple combination of α-tocopherol and α-tocopherol succinates as a component of poorly immune-stimulative LNPs (formed with a myristic acid-scaffold ssPalm) did not enhance cytokine production [18].The covalent linkage of α-tocopherol and a tertiary amine into a single molecular structure and/or the dimerization of α-tocopherol could be key factors in the adjuvant effect.Thus, it is plausible that the action of the LNPs ssPalmE involves a mechanism that differs from that of AS03.
The other molecular design integrated in ssPalmE-P4C2 is the introduction of a phenyl ester.The degradability of ionizable lipids in the body has been investigated as a strategy for reducing adverse side effects caused by the accumulation of lipid-like materials.The introduction of ester bonds and/or disulfide bonds is one of the common strategies for achieving the biodegradation of an LNP [15].It was reported that the combination of disulfide bonds and a phenyl ester moiety synergistically enhances the transfection activity of mRNA by inducing an intraparticle self-degradation that occurs within the limited intraparticle spaces.This reaction induces the release of the nucleic acid cargo [17].Actually, an siRNA-LNP that contains an oleic-acid-based self-degradable ssPalm (ssPalmO-Phe-P4C2) showed an ED 50 of 0.0044 mg/kg siRNA in the liver when administered via an i.v.injection [15].Comparing the knockdown activity of ssPalmE-P4C2 with that of ssPalmO-Phe-P4C2, the findings suggested that the efficiencies of nucleic acid delivery for these two materials were significantly different.From this finding, we concluded that the combination of vitamin E scaffolds and phenyl esters is a strategy that can satisfy both immunostimulatory properties and nucleic acid delivery efficiency.The improvement in the luciferase activity and CTL activity of LNP ssPalmE-Phe over LNP ssPalmE suggested that the release of mRNA promoted by self-degradability is an important factor for improving the efficacy of an RNA vaccine.The LNP ssPalmE-Phe (RtoU) also demonstrated strong CTL activity and a significant suppression of E.G7-OVA tumor growth (Figure 7).These observations suggest that ssPalmE-Phe-P4C2 would be a reasonable candidate for use in designing an RNA vaccine that focuses on the activation of cellular immunity.
It is noteworthy that the mRNAs used in this study were unmodified/unpurified.However, in the case of ionizable lipids with vitamin E scaffolds, the hydrophobic scaffold contributed significantly to the adjuvant activity of the LNPs [18].The contribution of mRNA to the adjuvant activity was negligible.Therefore, ssPalmE-Phe-P4C2, rather than the IVT-mRNA, is considered to be responsible for the immunostimulatory properties of the mRNA-LNPs [18].Since the improvement in the quality of mRNA can positively affect the vaccine activity of an RNA vaccine [29], the combination of chemically modified and purified mRNA with LNPs ssPalmE-Phe (RtoU) would be a potent strategy for the further development of cancer vaccines.
The ready-to-use (RtoU) formulation, a freeze-drying-based preparation method of mRNA-LNPs, was then applied to the LNPs ssPalmE-Phe to further develop them as a convenient nucleic acid carrier.Freeze-drying has been extensively used in pharmaceutical industries to improve the stability and shelf life of drug products [19,30,31].However, the stresses associated with freezing and drying can damage biomolecules unless an appropriate cryoprotectant and lipid composition are used [32].In this study, the successful application of an RtoU formulation produced mRNA-LNPs that allow for the post-encapsulation of the desired nucleic acids to be achieved via simple rehydration and incubation.The LNP ssPalmE-Phe (RtoU) offers handling practicality and a high rate of encapsulation (>90%) with a minimum loss of nucleic acids.The limitation of the LNP ssPalmE-Phe (RtoU) formulation involves the concentration (1.0 µg mRNA/200 µL) and scale (1.0 µg mRNA/vial) of the resultant mRNA-LNPs.Although this is sufficient for the vaccine experiment in mice, this point should be improved from the viewpoint of other applications.
Sucrose acts as a cryoprotectant, which helps to maintain the stability and integrity of LNPs during the freeze-drying process.The Pfizer/BioNTech and Moderna COVID-19 mRNA vaccines both used sucrose as a cryoprotectant to protect and stabilize the LNPs while in a deep-frozen state (−80 • C) during distribution [33][34][35][36].The Food and Drug Administration authorized the most recent formula of the Pfizer/BioNTech and Moderna COVID-19 mRNA vaccines (2023-2024 Formula), which corresponds to the Omicron variant XBB.1.5 of SARS-CoV-2.In this recent formula, the Pfizer/BioNTech COVID-19 mRNA vaccine used 31 mg of sucrose in each 0.3 mL dose (103.3 mg/mL) [37]; meanwhile, the Moderna COVID-19 mRNA vaccine used 21.8 mg of sucrose in each 0.25 mL dose (87.2 mg/mL) [38].Compared to a frozen vaccine product, such as the COVID-19 mRNA vaccines, a much higher sucrose concentration is needed to stabilize a lyophilized vaccine product.It was mentioned that sugar molecules replace the water between the hydrophilic heads of phospholipids during lyophilization, thus lowering the phase transition temperature and preventing a gel-to-liquid phase transition [31].It was also suggested that sugar forms glass matrixes, trapping phospholipids upon the removal of water, which prevents lipid aggregation and damage by ice crystals [31,39].In this study, sucrose was used as a cryoprotectant for the LNPs ssPalmE-Phe (RtoU) following a previously reported procedure [20,40].The dent or shrinkage appearance of the LNPs ssPalmE-Phe (RtoU) is probably related to the formulation process, in which the secondary drying process was apparently too rapid [22].The amount of sucrose and/or the freeze-drying process should be optimized for each application.In our experiments, the dent appearance of the lyophilized product had no effect on the product's quality.This is consistent with the general notion that a dent appearance is acceptable [22].The pH of the buffer is another important factor in preparing an LNP, especially for the post-encapsulation of mRNA.The electrostatic interaction between ionizable lipids and nucleic acids is probably the main driving force in the post-encapsulation process [20].It should be noted here that the buffer pH (5.0) together with a high concentration of sucrose (320 mg/mL) produced preferable particle properties (Table 2) and a high immune activation activity (Figure 5).
The established RtoU formulation (ssPalm/helper lipid/cholesterol = 52.5/7.5/40)[20] was found to be the most suitable lipid composition for the LNPs ssPalmE-Phe , which demonstrated stronger immune activation activity compared to the other formulations (Figure 6).This observation is closely related to the cholesterol composition.Cholesterol is known to have the ability to provide cryoprotection for LNPs during freeze-drying by maintaining the stability of lipid formation and preventing the risk of leakage of contents [41,42].In addition, it was revealed that a cholesterol content of at least 40% in the LNPs ssPalm was essential for achieving a high transfection activity of the phenyl-type ionizable lipid [17].The ONPATTRO ® or Patisiran (Alnylam Pharmaceutical, Cambridge, MA, USA) also utilizes a cholesterol content > 30% (38.5%) in its LNP formulation for siRNA delivery [33].Moreover, the current COVID-19 mRNA vaccines by Pfizer/BioNTech and Moderna also utilize a cholesterol content >30% in their LNP formulation, with 42.7% and 38.5% cholesterol, respectively [33].Although the exact composition of cholesterol varies depending on the type of ionizable lipid and its delivery purpose, a cholesterol content of at least around 40% in the LNP formulation might be important in terms of exerting its mRNA delivery efficiency.The limitation of the LNP(RtoU) formulation is the concentration (1.0 µg/200 µL) and scale (1.0 µ/vial) of the resultant mRNA-LNPs.Although this is enough for the vaccine experiment in mice, this point should be improved from the viewpoint of other applications.
Phospholipids are also included as helper lipids to provide particle stability and delivery efficiency [43].DOPE is a cone-shaped lipid with high fusogenic activity, while DOPC functions to stabilize the LNPs through its overall cylindrical shape.Both lipids were used to form the microfluidic-mixer-type (Figure 3) or the RtoU-type (Figure 4) LNPs ssPalmE-Phe .As a result, the use of DOPE was revealed to be important for achieving higher activity of cytotoxic T cells.The findings indicated that the CTL activity of the LNPs ssPalmE-Phe (RtoU) tended to increase when the DOPE ratio was reduced.However, the complete removal of the helper lipid was shown to be detrimental to the CTL activity of the LNPs ssPalmE-Phe (RtoU).This indicates there is a delicate balance between the ionizable lipid, the helper lipid, and the amount of cholesterol needed to achieve an optimum lipid composition.When the cholesterol ratio was fixed, the DOPE ratio was accompanied by an increase in the ssPalmE-Phe-P4C2 ratio.Thus, the amount of ionizable lipid holds priority in the lipid composition, which determines the immune activation activity of the LNPs ssPalmE-Phe (RtoU).

Conclusions
Ready-to-use-type LNPs ssPalmE-Phe offer a convenient form of an mRNA-based vaccine carrier.The LNPs ssPalmE-Phe (RtoU) were capable of post-encapsulating the intended mRNA antigens through a simple rehydration/incubation step.The efficacy of the LNPs ssPalmE-Phe (RtoU) to significantly suppress tumor progression indicated their usefulness in cancer vaccine development.Therefore, this RtoU technology would be suitable for easily screening novel antigens/neo-antigens and/or confirming a proof-of-concept as to whether the candidate gene actually functions as an antigen for cancer therapy.

Figure 1 .
Figure 1.Chemical structure of lipid components and schematic illustration of the experimental design.(a) The molecular structures of ssPalmE-Phe-P4C2, ssPalmE-P4C2, DOPE, DOPC, cholesterol, and DMG-PEG2000 are shown.(b) Experimental design of LNPssPalmE-Phe(RtoU) preparation; the empty LNPs were prepared through microfluidic mixing and then lyophilized to produce dry empty LNPs.The mRNA-encapsulated LNPs could be obtained via the reconstitution (rehydration) of the dry LNPs with the mRNA solution in water.

Figure 1 .
Figure 1.Chemical structure of lipid components and schematic illustration of the experimental design.(a) The molecular structures of ssPalmE-Phe-P4C2, ssPalmE-P4C2, DOPE, DOPC, cholesterol, and DMG-PEG 2000 are shown.(b) Experimental design of LNP ssPalmE-Phe (RtoU) preparation; the empty LNPs were prepared through microfluidic mixing and then lyophilized to produce dry empty LNPs.The mRNA-encapsulated LNPs could be obtained via the reconstitution (rehydration) of the dry LNPs with the mRNA solution in water.

Figure 2 .
Figure 2. In vitro and in vivo gene expressions of LNPsssPalmE and LNPsssPalmE-Phe prepared with a microfluidic device.(a) Physicochemical properties of LNPsssPalmE and LNPsssPalmE-Phe.In vitro gene expression after transfection of mLuc-LNPs at a dose of 0.8 µg of mRNA in (b) BMDCs (8.0 × 10 5 cells) and (c) RAW 264.7 cells (2.0 × 10 5 cells).Luciferase activity is represented as a relative light unit (RLU/mg protein), calculated from the luminescence intensities and protein content of the cell lysates.(d,e) Quantification and imaging results of the in vivo mLuc-LNP gene expression in C57BL/6J mice through immunization (s.c.) of 1.0 µg of mRNA.Six hours after administration, Dluciferin potassium (3 mg/200 µL/mouse) was administered (i.p.), and the luminescence intensities were measured using an IVIS device.The scatter graph represents the individual value; the bar graph represents the mean with SD (n = 3); * p < 0.05; ** p < 0.01 (two-tail unpaired t-test).The measurements in the in vitro experiments were conducted in triplicate.

Figure 2 .
Figure 2. In vitro and in vivo gene expressions of LNPs ssPalmE and LNPs ssPalmE-Phe prepared with a microfluidic device.(a) Physicochemical properties of LNPs ssPalmE and LNPs ssPalmE-Phe .In vitro gene expression after transfection of mLuc-LNPs at a dose of 0.8 µg of mRNA in (b) BMDCs (8.0 × 10 5 cells) and (c) RAW 264.7 cells (2.0 × 10 5 cells).Luciferase activity is represented as a relative light unit (RLU/mg protein), calculated from the luminescence intensities and protein content of the cell lysates.(d,e) Quantification and imaging results of the in vivo mLuc-LNP gene expression in C57BL/6J mice through immunization (s.c.) of 1.0 µg of mRNA.Six hours after administration, D-luciferin potassium (3 mg/200 µL/mouse) was administered (i.p.), and the luminescence intensities were measured using an IVIS device.The scatter graph represents the individual value; the bar graph represents the mean with SD (n = 3); * p < 0.05; ** p < 0.01 (two-tail unpaired t-test).The measurements in the in vitro experiments were conducted in triplicate.

Figure 3 .
Figure 3. CTL activity of the LNPsssPalmE and LNPsssPalmE-Phe prepared using a microfluidic device.The mOVA-LNPsssPalmE and mOVA-LNPsssPalmE-Phe, with either DOPE or DOPC as helper lipids, were evaluated for their immune activation activity via a CTL assay.The CTL assays of the mOVA-LNPs were conducted in C57BL/6J mice via immunization (s.c.) of 0.05 µg of mRNA.On day 7 after immunization, the mice were injected (i.v.) with equal amounts of CFSE hi -labelled splenocytes (SI-INFEKL OVA epitope) and CFSE low -labelled splenocytes (naïve).The spleen was collected on Day 8, and the % lysis of splenocytes was quantified via flow cytometry.The scatter graph represents individual values; the bar graph represents the mean with SD (n = 3).N.S.: not significant; * p < 0.05 (one-way ANOVA followed by the Bonferroni test against the original LNPssPalmE).

Figure 3 .
Figure 3. CTL activity of the LNPs ssPalmE and LNPs ssPalmE-Phe prepared using a microfluidic device.The mOVA-LNPs ssPalmE and mOVA-LNPs ssPalmE-Phe , with either DOPE or DOPC as helper lipids, were evaluated for their immune activation activity via a CTL assay.The CTL assays of the mOVA-LNPs were conducted in C57BL/6J mice via immunization (s.c.) of 0.05 µg of mRNA.On day 7 after immunization, the mice were injected (i.v.) with equal amounts of CFSE hi -labelled splenocytes (SIINFEKL OVA epitope) and CFSE low -labelled splenocytes (naïve).The spleen was collected on Day 8, and the % lysis of splenocytes was quantified via flow cytometry.The scatter graph represents individual values; the bar graph represents the mean with SD (n = 3).N.S.: not significant; * p < 0.05 (one-way ANOVA followed by the Bonferroni test against the original LNP ssPalmE ).

Figure 5 .
Figure 5. CTL activity of LNPsssPalmE-Phe(RtoU) prepared using different buffer pHs and sucrose co centrations.The CTL activity for combined buffer pH(s) and sucrose concentrations was evaluate and exhibited acceptable appearances and physicochemical properties, as shown in Table2.A CT assay of mOVA-LNPs(RtoU) was conducted in C57BL/6J mice via immunization (s.c.) with 0.1 µ of mRNA.The spleen was collected, and the % lysis of splenocytes was quantified via flow cytom etry.The scatter graph represents individual values; the bar graph represents the mean with SD = 3).N.S.: not significant; ** p < 0.01 (one-way ANOVA followed by SNK test).

Figure 5 .
Figure 5. CTL activity of LNPs ssPalmE-Phe (RtoU) prepared using different buffer pHs and sucrose concentrations.The CTL activity for combined buffer pH(s) and sucrose concentrations was evaluated and exhibited acceptable appearances and physicochemical properties, as shown in Table 2.A CTL assay of mOVA-LNPs(RtoU) was conducted in C57BL/6J mice via immunization (s.c.) with 0.1 µg of mRNA.The spleen was collected, and the % lysis of splenocytes was quantified via flow cytometry.The scatter graph represents individual values; the bar graph represents the mean with SD (n = 3).N.S.: not significant; ** p < 0.01 (one-way ANOVA followed by SNK test).

Table 2 .
Physicochemical properties of the LNPs ssPalmE-Phe (RtoU) prepared with different buffer pH(s) and sucrose concentrations.Size, PdI, and ZP were measured with Zetasizer Nano ZS.Encapsulation efficiency was measured with Ribogreen ® assay.The samples with asterisks (*) were evaluated for CTL activity.