Green Synthesis of Silver Nanoparticles Using Hypericum perforatum L. Aqueous Extract with the Evaluation of Its Antibacterial Activity against Clinical and Food Pathogens

The rapid development of nanotechnology and its applications in medicine has provided the perfect solution against a wide range of different microbes, especially antibiotic-resistant ones. In this study, a one-step approach was used in preparing silver nanoparticles (AgNPs) by mixing silver nitrate with hot Hypericum perforatum (St. John’s wort) aqueous extract under high stirring to prevent agglomeration. The formation of silver nanoparticles was monitored by continuous measurement of the surface plasma resonance spectra (UV-VIS). The effect of St. John’s wort aqueous extract on the formation of silver nanoparticles was evaluated and fully characterized by using different physicochemical techniques. The obtained silver nanoparticles were spherical, monodisperse, face-centered cubic (fcc) crystal structures, and the size ranges between 20 to 40 nm. They were covered with a capping layer of organic compounds considered as a nano dimension protective layer that prevents agglomeration and sedimentation. AgNPs revealed antibacterial activity against both tested Gram-positive and Gram-negative bacterial strains causing the formation of 13–32 mm inhibition zones with MIC 6.25–12.5 µg/mL; Escherichia coli strains were resistant to tested AgNPs. The specific growth rate of S. aureus was significantly reduced due to tested AgNPs at concentrations ≥½ MIC. AgNPs did not affect wound migration in fibroblast cell lines compared to control. Our results highlighted the potential use of AgNPs capped with plant extracts in the pharmaceutical and food industries to control bacterial pathogens’ growth; however, further studies are required to confirm their wound healing capability and their health impact must be critically evaluated.


Introduction
Recently, there has been an increase in the number of emerged antibiotic-resistant bacterial strains, the number of multidrug-resistant tumor cells, and the number of oxidative stress-associated disorders; an increase that elevates the burden of treating such ailments. Moreover, the usage of some synthetic drugs is associated with adverse effects on human for a further 30 min under stirring until completion of the reaction. By modifying all these conditions, we observed a small variation in the particle size (20-40 nm), while previously its range was between 20-60 nm. The coating of the nanoparticles and their stabilization by the plant phytochemical constituents was confirmed via chemical characterization and their antibacterial efficiency against clinical and food pathogenic isolates was evaluated and compared with that of the plant extract; their effect on the growth kinetics and killing time of tested Staphylococcus aureus was elaborated. Herein, the effect of H. perforatum-coated AgNPs on wound healing was investigated for the first time.

Chemicals and Instrumentation
Aerial parts of H. perforatum L. were collected in July-August from the Ghab Plain in Syria and harvested during the flowering season. The sample was identified and authenticated by Dr. Watfeh of the Faculty of Agriculture at Damascus University. Silver nitrate was purchased from Sigma Aldrich. Whatman filter paper (90 mm) was purchased from GE Healthcare Life Sciences (Freiburg Germany). Deionized water (18.2 MΩ cm, 25 • C) was obtained from a Millipore Mill-Q system, Sartorius (Goettingen, Germany).
The absorption spectra of AgNPs, immediately after synthesis, after one month, and after 8 months of storage, were recorded using a NanoDrop Spectrophotometer (ND1000 from PeQLab). Dynamic light scattering and Zeta potential using Bettersize S3 plus from Anton Paar was performed. This was followed by Fourier transform infrared spectrophotometry using a Bruker FT-IR Vertex 80 v spectrometer. X-ray diffraction (XRD) wase done using a Bruker D8 Advance diffractometer. A thermal gravimetric analysis (TGA) was performed using a TGA/DSC 3+ from Mettler-Toledo, from 25 to 1000 • C at a rate of 1 • C per minute with N 2 gas flow. Scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDXS) were done using a JEOL JSM-6700F. Transmission electron microscopy (TEM) was performed with the use of a JEOL JEM-2100F-UHR, at an acceleration voltage of 200 kV. The surface morphology was determined by the atomic force microscopy measurements (AFM, Nanosurf easyScan2, Liestal, Switzerland). The AFM measurements were performed in contact mode.
NTA measurements were performed with a NanoSight LM10 (NanoSight, Amesbury, UK) equipped with a sample chamber with a 635 nm laser and a Viton fluoroelastomer O-ring. The samples were injected into the sample chamber with sterile syringes (Carl Roth GmbH, Karlsruhe, Germany) until the liquid reached the tip of the nozzle. All measurements were performed at room temperature. The software used for capturing and analyzing the data was the NTA 2.0 Build 0033. The samples were measured for 60 s with manual shutter and gain adjustments. The "single shutter and gain mode" was used to capture the monodisperse AgNPs.

Synthesis of Hypericum Perforatum L. aqueous Extract Mediated Silver Nanoparticles (AgNPs) and Their Chemical Characterization
The synthesis of AgNPs was done as mentioned previously [4], with some modifications. Briefly, H. perforatum aerial parts were cleaned and ground to a fine powder. 3 g of the pulverized sample were boiled in 700 mL distilled water for 3 h, and the supernatant was filtered using a 0.22 µm membrane filter. The resulting yellow-brown extract was freeze-dried. Preparation of AgNPs was achieved by adding 10 mL of AgNO 3 (0.5 M) dropwise to plant extract solution (0.075 g of the plant extract in 40 mL deionized water) with stirring at 750 rpm and 80 • C. The formation of AgNPs was confirmed by the change of preparation color from pale yellow to dark brown, and the preparation was left in a dark place while cooling. The resulting nanoparticles were isolated, washed with deionized water, and centrifugated at 15,000 rpm for one hour, and the process was repeated at least four times. The purified AgNPs were chemically characterized using ultraviolet light spectroscopy (UV-VIS), dynamic light scattering (DLS) and zeta potential, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy disperse X-ray spectroscopy (EDX), atomic force microscopy (AFM) and nanoparticle tracking analysis (NTA).
The phytochemical constituents of H. perforatum extract, which might have a role in reducing AgNO 3 and stabilizing the formed AgNPs, were determined in a previous work by high-performance liquid chromatography equipped with a diode array detector (HPLC-DAD) coupled with UV-visible spectrophotometry at a full spectrum (200-800 nm) [46]. Detection was carried out at 260 nm for phloroglucinols, 590 nm for naphthodianthrones, and 350 nm for other flavonols, flavones, and chlorogenic acids.

Antibacterial Activity
The green synthesized AgNPs were tested for their antibacterial activity by a well diffusion test, and the lowest concentration needed to inhibit bacterial growth (MIC) was determined by a micro-broth dilution assay; both assays were performed as described in Al-Zereini [47]  and were identified by a BIOMÉRIEUX VITEK ® 2 system. Overnight, the bacterial culture was seeded in Muller Hinton agar plates (MHA) (Oxoid, Hampshire, UK) at a cell density of 10 6 cells/mL and in which 6 mm diameter wells were made using a sterile Cork borer. To each well in the bacterial agar plates, either 120 µL aliquots (100 µg/well), 60 µL aliquots (50 µg/well) from AgNPs preparation, or 100 µL aqueous plant extract (2.5 mg/well) were applied. As a positive control, streptomycin (10 µg/disc, Bio Basic Inc., Markham, ON, Canada) was used and the results are presented as means of triplicate tests ± SD.
However, in the micro-broth dilution assay, AgNPs and the positive control were tested starting from 100 µg/mL and 50 µg/mL, respectively. Samples were serially diluted in 100 µL Muller Hinton broth (MHB), to which 100 µL of the tested bacterial strain was added to each well at a cell density of 2 × 10 6 cells/mL. The preparation was incubated for 24 h at 37 • C. Aliquots from the preparations in micro-broth dilution assay, where there were no visible growths, were platted on agar plates with proper medium and incubation for 24-48 h to determine if the nanoparticle has a bactericidal or bacteriostatic effect.

Effect of AgNPs on Growth Kinetics of S. aureus
Based on the AgNPs antibacterial activity results, S. aureus was selected to study the effect of nanoparticles on the bacterial growth kinetics. S. aureus was cultivated at a cell density of 10 6 cells/mL in sterile test tubes containing 10 mL of MHB (Oxoid, UK). Different concentrations of AgNPs (1, 3, 6, 12, and 24 µg/mL) were added to bacterial culture test tubes with incubation for 24 h on an orbital shaker (Forma-Thermo electron cooperation, Waltham, MA, USA) at 37 • C and 150 rpm. A bacterial culture test tube without AgNPs was used as a negative control. During the cultivation period, 1 mL sample was withdrawn after 3 h intervals and up to 24 h to measure bacterial growth in terms of increase in the optical cell density at λ 600nm (OD 600nm ) using a UV/Vis spectrophotometer (Novaspec Model. 80-2088-64, Pharmacia Biotech, UK). The growth curve was established by plotting OD 600nm values against the treatment time. The specific growth rate was calculated according to the formula: µ = (ln(X 2 ) − ln(X 1 ))/(t 2 − t 1 ) where µ is the specific growth rate, X 1 and X 2 are the OD 600nm at time t 1 and t 2 of the bacterial culture. The experiment was performed in triplicate and the results are presented as means of three independent tests ± SD.

Estimation of Antibacterial Activity of AgNPs in Terms of CFU
Different concentrations of AgNPs (1, 3, 6, 12, and 24 µg/mL) were used to evaluate their effect against S. aureus growth in terms of colony-forming units (CFU) on Muller Hinton agar plates. The bacterium was grown overnight and then diluted to a cell density equivalent to 0.5 McFarland (≈1.5 × 10 8 CFU/mL). They were diluted to 10 5 fold and 100 µL were spread on solid agar plates containing different AgNPs concentrations. The plates were incubated for 24 h at 37 • C and the grown colonies were photographed.

Time-Kill Kinetics Assay
The time-kill assay was performed for the S. aureus culture exposed to 1 2 MIC, MIC, and 2 MIC concentrations of AgNPs following the CLSI guideline [48]. An overnight bacterial culture was centrifuged at 4000 rpm for 1 h to harvest the cells (Combi-514R, Hanil Scientific Inc., Gimpo, Korea). The harvested cells were suspended in sterile normal saline (0.085% NaCl, w/v) and adjusted to 0.5 McFarland (≈1.5 × 10 8 CFU/mL). Aliquots of 1 mL from the prepared suspension were inoculated into test tubes containing 10 mL MHB (Oxoid, UK), with final concentrations of the AgNPs equal to 1 2 MIC, MIC, and 2 MIC. All tubes were incubated at 37 • C and samples were taken at intervals of 30 min starting at the time of inoculation and up to 300 min. The estimation of the number of surviving bacteria was carried out by spreading 100 µL from appropriate bacterial dilutions in sterile normal saline onto the top of MHA plates with incubation at 37 • C for 24 h; the number of bacterial colonies was counted and expressed as CFU/mL. Time-kill curves were constructed by plotting the Log 10 (CFU/mL) against the exposure time (minutes). The assay was performed in triplicate and the results are presented as means of three independent tests ± SD.

Wound Migration Assay
A wound migration assay was performed using the normal human dermal fibroblast cell line (ATCC ® PCS-201-012) and as described in Wadhwani et al. [49]. Cells were cultured in a 12-well microtiter plate as a monolayer, and they were seeded at a cell density of 5 × 10 4 cells/mL. The cells were grown for 24 h in Dulbecco's Modified Eagle Medium (DMEM; Gibco, Mannheim, Germany) supplemented with 10% heat-inactivated fetal calf serum (Gibco, Germany), L-glutamine, 65 µg/mL of penicillin G, and 100 µg/mL of streptomycin sulfate. They were incubated at 37 • C in a humidified atmosphere containing 5.0% CO 2 . A scratch of a uniform size was made in each well using a sterile micro-tip; the culture medium was aspirated from each well, the well was washed with a sterile phosphate buffer solution (pH 7.4), and fresh media with different concentrations of AgNPs (1, 3, 5, and 10 µg/mL) were added to each well seeded with the cell line. Two wells containing a fresh medium without the test compounds were used as untreated controls. The culture was photographed at 0 time and after 24 h using an inverted microscope (Nikon, Shinjuku-ku, Japan). Wound migration (closure) was measured by the following equation: % Wound migration = 1 − (area of the wound at T S /area of the wound at T 0 ) × 100 where T S is 24 h from wounding and T 0 is 0 h wounding.

Chemical Characterization of Hypericum perforatum L. Phytochemicals-Capped AgNPs
The aqueous extract is used as a reducing agent for silver ions into metallic silver, as well as a stabilizer to protect the silver nanoparticles (AgNPs) and prevent their aggregating. During the preparation process, the formation of the metallic silver particles via the silver ions (Ag + ) reduction with the St John's wort aqueous extract was observed by the color change to a light or dark brown. Figure 1a shows the UV-Vis spectra of phyto-capped AgNPs at various times (fresh, one month, and eight months after preparations). The absorption bands with a maximum wavelength of 401, 405, and 425 nm are assigned to the plasmon resonance of metallic AgNPs, indicating the existence of monodispersed spherical silver nanoparticles in the colloidal solution. Several research groups observed the surface plasmon resonance absorption band for AgNPs synthesized by similar methods with different plant extracts between 400-430 nm [50,51]. In contrast, the observed broad absorption band in the UV region at λ max around 200 nm could be assigned to the presence of different organic compounds adsorbed on the surface of the AgNPs during the preparation process, such as flavonoids, phenolic acids, unsaturated groups, and heteroatoms such as S, N, O [52,53]. It is worth mentioning that the dispersion of the AgNPs is stable and that further aggregation after one month or eight months of preparation ( Figure 1a) does not affect the stability of the nanoparticles, since the redshift of the absorption bands in the UV-Vis spectra were very small. These results revealed the formation of very small silver nanoparticles during the preparation process [54,55] and coincided with the observation of Shankar et al. [56] on the small size of formed AgNPs using Azadirachta indica leaf broth and their stability for one month.
Pharmaceutics 2022, 14, x FOR PEER REVIEW 7 of 20 cm −1 correspond to C-O-C stretching in aromatic rings [64]. Both peaks at around 1385 and 1445 cm −1 are responsible for C-H stretching [65]. The peak at 1527 cm −1 is a response to stretching OH and C=C aromatic. The bands near 1617 cm −1 (sometimes 1640 cm −1 ) and near 1734 cm −1 refer to C=O, C-H, and C=O stretching in the aromatic ring. Bands in the range of 1923-2000 cm −1 prove the existence of an aromatic ring, while bands in the range of 2900-2990 cm −1 correspond to C-H stretching and the broad and intense band near 3400 cm −1 indicates the presence of O−H stretching in phenols [64,66,67]. The bands at 510 cm −1 and 590 cm −1 are attributed to the metal-ligand frequency stretching which formed due to the interaction between biomolecules and the AgNPs surfaces [68,69].  It is believed that the phloroglucinols (hyperforin, Adhyperforin), the naphthodianthrone hypericin, the flavonoids (rutin, quercetin, quercitrin, quercitrin-hydrate, hyperoside), the bioflavonoid biapigenin, and the phenolic acid chlorogenic acid identified previously in the aqueous St John's wort extract [46] played a role in the silver reduction and that the stabilization of the formed nanoelement might endow the AgNPs with advantageous bioactivities, such as antibacterial, antioxidant, and anti-cancer activity. Such an assumption is reinforced by the finding of Pradeep et al. [45], where the polar fraction containing phenolic acids and flavonoids was involved in the reduction of Ag+ ions, while the lower polar one containing biflavonoids, phloroglucinols, and naphthodianthrones acted as a capping agent. Moreover, in the same context, the presence of hypericin, pseudohypericin, hyperforin, and flavonoids in H. perforatum extracts and low polar compounds on the surface of NPs contributes to their antioxidant and antibacterial activities [39][40][41]57].
The dynamic light scattering technique was used to estimate the mean size, size distribution, and agglomeration potential of AgNPs [58]. Figure 1b shows the size of the resulting AgNPs depending on the relative frequency number weight (%). The hydrodynamic diameter distribution of green synthesized AgNPs in colloidal solution was found in the range of 15-30 nm with a polydispersity index (PDI) of 0.19. The zeta potential measurements showed that AgNPs carry a negative surface charge of −19 mv value in the colloidal solution ( Figure 1c). The negatively charged surfaces of AgNPs might be attributed to their decoration with phenolic compounds, carboxylated polysaccharides, and the side chain of some amino acid residues [59,60], a charge that leads to less agglomeration and better physical stability. It is documented that the nanoparticles are well stabilized in colloidal solutions when the stabilizer on their surface is well adsorbed [61]. The dissociation of some H + and total protonation of the hydroxyl groups of phytochemicals favor the repulsion between the nanoparticles, which discourages their aggregation.
The ATR-IR diagram shown in Figure 1d confirmed the existence of St. John's wort extracted compounds on the surfaces of silver nanoparticles as a reducer and stabilizer. The presence of moieties from Naphthodianthrone, Phloroglucinol, flavonoids, and bioflavonoids derivatives, identified in our previous report on St. John's wort extract [46], cause the appearance of well-known signals in the infrared region of the electromagnetic spectrum such as hydroxyl groups, alkyl moiety, and aromatic rings. Indeed, the positions of these bands in the ATR-FTIR spectra are considered a sensitive indicator of the conformational changes in the structure of the plant compounds due to their adsorption on the surfaces of silver nanoparticles [62]. The band appears in the region 675-900 cm −1 corresponding to C-H aromatic out of the plane [63]. The weak bands at 1230, 1242, and 1251 cm −1 correspond to C-O-C stretching in aromatic rings [64]. Both peaks at around 1385 and 1445 cm −1 are responsible for C-H stretching [65]. The peak at 1527 cm −1 is a response to stretching OH and C=C aromatic. The bands near 1617 cm −1 (sometimes 1640 cm −1 ) and near 1734 cm −1 refer to C=O, C-H, and C=O stretching in the aromatic ring. Bands in the range of 1923-2000 cm −1 prove the existence of an aromatic ring, while bands in the range of 2900-2990 cm −1 correspond to C-H stretching and the broad and intense band near 3400 cm −1 indicates the presence of O−H stretching in phenols [64,66,67]. The bands at 510 cm −1 and 590 cm −1 are attributed to the metal-ligand frequency stretching which formed due to the interaction between biomolecules and the AgNPs surfaces [68,69].
The XRD pattern shown in Figure 1e proves the formation of the nanocrystal structure of the green synthesized silver nanoparticle. The four special diffraction peaks at 2θ values 38.12 • , 46.26 • , 64.45 • , and 77.38 • which correspond to the planes (111), (200), (220), and (311), respectively, possess well-defined characteristic peaks corresponding to the facecentered cubic structure of pure metallic silver (JCPDS file No. 04-0783) [70,71]. The ratio between the intensities of the diffraction peaks of planes (200) and (111) is much lower than the typical value (0.52), revealing that the (111) plane is the predominant orientation [72], while the unassigned diffraction peaks can be related to the formation of the calcium nanocomposites [73]. In a similar study, the formation of calcium nanocomposite was observed during the biosynthesis of AgNPs by using Carduus crispus extract [74]. It is worth mentioning that the elemental analysis for Hypericum perforatum revealed that calcium exists in the highest concentration (5600 µg/g in leaves and 2500 µg/g in flowers) compared to the other elements in this plant [75], which indicates that the source of the calcium in our sample originated from the plant extract.
The broadening of the diffraction pattern peaks indicates the small average crystallite sizes of AgNPs, which can be measured using Debye-Scherrer's equation [76]. The average size of crystalline silver nanoparticles was found to be around 12 nm.
The TGA analysis of phytochemical-coated AgNPs at 25 to 1000 • C with a heating rate of 1 • C min is presented in Figure 1f, and revealed three decomposition stages; accumulated water molecules absorbed on the surface of the nanoparticles are lost in the first stage after heating to 100 • C, the temperature was raised from 100 • C to 160 • C in the second stage, and heating was extended up to 600 • C in the third stage. The initial thermal deterioration of all the phytochemicals on the surface of these nanoparticles occurred and then the complete degradation continued. Finally, the ash and the mineral component remained due to the use of an inert atmosphere [77,78].
The scanning electron microscopy (SEM) images of phytochemical coated AgNPs showed that most of the silver nanoparticles were spherical and monodisperse with sizes ranging from 20-40 nm, while other particles were aggregated with each other without a specific shape. This aggregation is a result of the network of organic compounds from St. John's wort extract present as a capping protective layer on the surfaces of the silver nanoparticles (Figure 2a,b). Moreover, scanning transmission electron microscopy darkfield (STEM-DF) images (Figure 2c) of green synthesized silver nanoparticles confirmed that the particles' sizes were about 20-40 nm and they were spherical and monodispersed. and (311), respectively, possess well-defined characteristic peaks corresponding to the face-centered cubic structure of pure metallic silver (JCPDS file No. 04-0783) [70,71]. The ratio between the intensities of the diffraction peaks of planes (200) and (111) is much lower than the typical value (0.52), revealing that the (111) plane is the predominant orientation [72], while the unassigned diffraction peaks can be related to the formation of the calcium nanocomposites [73]. In a similar study, the formation of calcium nanocomposite was observed during the biosynthesis of AgNPs by using Carduus crispus extract [74]. It is worth mentioning that the elemental analysis for Hypericum perforatum revealed that calcium exists in the highest concentration (5600 μg/g in leaves and 2500 μg/g in flowers) compared to the other elements in this plant [75], which indicates that the source of the calcium in our sample originated from the plant extract.
The broadening of the diffraction pattern peaks indicates the small average crystallite sizes of AgNPs, which can be measured using Debye-Scherrer's equation [76]. The average size of crystalline silver nanoparticles was found to be around 12 nm.
The TGA analysis of phytochemical-coated AgNPs at 25 to 1000 °C with a heating rate of 1 °C min is presented in Figure 1f, and revealed three decomposition stages; accumulated water molecules absorbed on the surface of the nanoparticles are lost in the first stage after heating to 100 °C, the temperature was raised from 100 °C to 160 °C in the second stage, and heating was extended up to 600 °C in the third stage. The initial thermal deterioration of all the phytochemicals on the surface of these nanoparticles occurred and then the complete degradation continued. Finally, the ash and the mineral component remained due to the use of an inert atmosphere [77,78].
The scanning electron microscopy (SEM) images of phytochemical coated AgNPs showed that most of the silver nanoparticles were spherical and monodisperse with sizes ranging from 20-40 nm, while other particles were aggregated with each other without a specific shape. This aggregation is a result of the network of organic compounds from St. John's wort extract present as a capping protective layer on the surfaces of the silver nanoparticles (Figure 2a,b). Moreover, scanning transmission electron microscopy dark-field (STEM-DF) images (Figure 2c) of green synthesized silver nanoparticles confirmed that the particles' sizes were about 20-40 nm and they were spherical and monodispersed. The morphology and surface roughness of AgNPs was confirmed by atomic force microscopy (AFM). The magnification in particle size complexity is proportional to the size of the AFM tip and in sample preparation [79,80]. Figure 2d,e revealed spherical silver nanoparticles with grain diameters ranging from 20-40 nm, which agreed with those obtained from the DLS results. Larger scans screening a few micrometers resulted in a maximum peak-to-valley distance of 30 nm and a root mean square (RMS) roughness of 10 nm. Figure 2f revealed the relative amounts of the elements in prepared AgNPs. EDX diagrams recorded specific elemental silver peaks at 2.8 keV, 3.5 keV, and 3.8 keV identical to the binding energies of Ag-L, Ag-M. This confirmed the existence of elemental silver in the resulting final colloidal solutions. The presence of the carbon and oxygen peaks is related to the organic layer from the St John's wort plant, which is present on the surfaces of nanoparticles as a protective agent. As shown in Figure 2f, the relative amounts of carbon are much larger than others because of the carbon from the holder.
Moreover, the average diameter of AgNPs in the colloidal solution was determined by the nanoparticle tracking analysis (NTA), an analytical technique that is considered more precise than the dynamic light scattering method used to evaluate particle size distribution [81,82]. The particle size distribution calculated using this technique revealed monodisperse silver nanoparticles with an average diameter of 20-40 nm (Figure 3), which confirmed the DLS results.
The morphology and surface roughness of AgNPs was confirmed by atomic force microscopy (AFM). The magnification in particle size complexity is proportional to the size of the AFM tip and in sample preparation [79,80]. Figure 2d,e revealed spherical silver nanoparticles with grain diameters ranging from 20-40 nm, which agreed with those obtained from the DLS results. Larger scans screening a few micrometers resulted in a maximum peak-to-valley distance of 30 nm and a root mean square (RMS) roughness of 10 nm. Figure 2f revealed the relative amounts of the elements in prepared AgNPs. EDX diagrams recorded specific elemental silver peaks at 2.8 keV, 3.5 keV, and 3.8 keV identical to the binding energies of Ag-L, Ag-M. This confirmed the existence of elemental silver in the resulting final colloidal solutions. The presence of the carbon and oxygen peaks is related to the organic layer from the St John's wort plant, which is present on the surfaces of nanoparticles as a protective agent. As shown in Figure 2f, the relative amounts of carbon are much larger than others because of the carbon from the holder.
Moreover, the average diameter of AgNPs in the colloidal solution was determined by the nanoparticle tracking analysis (NTA), an analytical technique that is considered more precise than the dynamic light scattering method used to evaluate particle size distribution [81,82]. The particle size distribution calculated using this technique revealed monodisperse silver nanoparticles with an average diameter of 20-40 nm (Figure 3), which confirmed the DLS results.

Evaluation of Biological Activities of Hypericum perforatum Mediated AgNPs
AgNPs revealed a broad-spectrum antibacterial activity against Gram-positive and negative tested bacterial strains, except for both E. coli strains that were resistant to the tested nanoparticles. The mean diameters of inhibition zones and the MIC values are presented in Table 1. According to the measured inhibition zones, Gram-positive bacteria Figure 3. Nanoparticle tracking analysis of phytochemicals-capped AgNPs. Particle size vs. the number of particles (a); representative nanoparticle tracking analysis video frame (b), particle size vs. relative intensity (c), and the number of particles vs. particle size and light scattering intensity (3D plot) are shown in (d).

Evaluation of Biological Activities of Hypericum perforatum Mediated AgNPs
AgNPs revealed a broad-spectrum antibacterial activity against Gram-positive and negative tested bacterial strains, except for both E. coli strains that were resistant to the tested nanoparticles. The mean diameters of inhibition zones and the MIC values are presented in Table 1. According to the measured inhibition zones, Gram-positive bacteria were more susceptible to AgNPs than the Gram-negative ones in a concentration-dependent manner with less effectiveness against B. subtilis among other Gram-positive species; they caused the formation of 13.3-32 mm inhibition zones in Gram-positive bacteria and 13-19.7 mm in P. aeruginosa and K. pneumonoiae at the highest tested concentration (100 µg/well). The concentration required to cause the absence of growth in sensitive bacterial strains ranged from 6.25-12.5 µg/mL (MIC), with a bactericidal effect in most. Intriguingly, the plant extract did not cause inhibition in the growth of all tested bacterial strains. Figure 4 reveals the effect of AgNPs and plant extract on the growth of S. aureus and B. cereus in the agar diffusion test. 13-19.7 mm in P. aeruginosa and K. pneumonoiae at the highest tested concentration (100 μg/well). The concentration required to cause the absence of growth in sensitive bacterial strains ranged from 6.25-12.5 μg/mL (MIC), with a bactericidal effect in most. Intriguingly, the plant extract did not cause inhibition in the growth of all tested bacterial strains. Figure 4 reveals the effect of AgNPs and plant extract on the growth of S. aureus and B. cereus in the agar diffusion test.  The ineffectiveness of H. perforatum aqueous extract to inhibit any of the tested bacterial strains concurred with previous reports on the inability of the plant leaf extract from H. perforatum [42] and different part extracts of Carduus crispus [74] to cause bacterial growth inhibition. Moreover, the antibacterial activity of AgNPs toward both Gram types of bacteria in the present study was in line with the previously reported broad-spectrum antibacterial activities of the nanoparticles in several studies, and was due to their ability to target and affect more than one cellular site and modulate the intracellular signaling and physiological pathways within the cell leading to their death [20,83]. In similar studies, AgNPs biosynthesized using extracts of H. perforatum [41,42], Hypericum hookerianum [84], Hypericum scabrum [85], Origanum vulgare [86], and Carduus crispus [74] exhibited activity against both Gram-negative and Gram-positive bacteria.
Also in line with our results, Gram-positive bacteria were documented to be slightly more sensitive than the negative ones to the tested nanoparticles [87][88][89][90]. Controversial The ineffectiveness of H. perforatum aqueous extract to inhibit any of the tested bacterial strains concurred with previous reports on the inability of the plant leaf extract from H. perforatum [42] and different part extracts of Carduus crispus [74] to cause bacterial growth inhibition. Moreover, the antibacterial activity of AgNPs toward both Gram types of bacteria in the present study was in line with the previously reported broad-spectrum antibacterial activities of the nanoparticles in several studies, and was due to their ability to target and affect more than one cellular site and modulate the intracellular signaling and physiological pathways within the cell leading to their death [20,83]. In similar studies, AgNPs biosynthesized using extracts of H. perforatum [41,42], Hypericum hookerianum [84], Hypericum scabrum [85], Origanum vulgare [86], and Carduus crispus [74] exhibited activity against both Gram-negative and Gram-positive bacteria.
Also in line with our results, Gram-positive bacteria were documented to be slightly more sensitive than the negative ones to the tested nanoparticles [87][88][89][90]. Controversial results were obtained by other studies that reveal more sensitivity of Gram-negative bacteria than Gram-positive ones toward tested AgNPs [86,91,92]. Interestingly, it was postulated that Ag + ions from the AgNPs have different targets between Gram-negative and positive bacteria [93], though the exhibited activity of AgNPs synthesized by Carduus crispus on both Gram-types of bacteria was not affected by the difference in the bacterial wall [92].
As S. aureus was the most susceptible bacterial test strain to AgNPs, it was selected to estimate the effect of nanoparticles on the growth kinetics of the bacterium ( Figure 5). As the concentration of the nanoparticle increases, a decrease in the OD 600nm value was noticed in comparison to the control culture and at different time intervals of sampling. Remarkably, the maximum cell density attained was about 50% of that reached by the control culture as the bacterium was exposed to almost 1 2 MIC concentration (6 µg/mL). Furthermore, at 12 µg/mL and 24 µg/mL, the growth of S. aureus was inhibited with an undetectable increase in the OD 600nm value throughout the whole period of cultivation (24 h). Such a finding indicated that the AgNPs have a toxic effect on the tested bacterium and their toxicity increase at higher concentrations of the nanoparticles. Intriguingly, the specific growth rate of S. aureus in the presence of AgNPs declined with an increase in the tested nanoparticle concentrations to 6 µg/mL; at concentrations over 6 µg/mL, the specific growth rate was almost zero, highlighting the higher toxicity of the nanoparticles on the bacterium ( Table 2). The specific growth rate was decreased from 0.43 h −1 in the control culture to 0.10 h −1 in the culture exposed to 6 µg/mL AgNPs. In terms of CFU, as the concentration of nanoparticles increased in Muller Hinton agar plates, there was a decrease in the number of growing colonies that were absent starting from concentrations ≥12 µg/mL, indicating a concentration-dependent antibacterial activity of AgNPs ( Figure 6). [92].
As S. aureus was the most susceptible bacterial test strain to AgNPs, it was selected to estimate the effect of nanoparticles on the growth kinetics of the bacterium ( Figure 5). As the concentration of the nanoparticle increases, a decrease in the OD600nm value was noticed in comparison to the control culture and at different time intervals of sampling. Remarkably, the maximum cell density attained was about 50% of that reached by the control culture as the bacterium was exposed to almost ½ MIC concentration (6 μg/mL). Furthermore, at 12 μg/mL and 24 μg/mL, the growth of S. aureus was inhibited with an undetectable increase in the OD600nm value throughout the whole period of cultivation (24 h). Such a finding indicated that the AgNPs have a toxic effect on the tested bacterium and their toxicity increase at higher concentrations of the nanoparticles. Intriguingly, the specific growth rate of S. aureus in the presence of AgNPs declined with an increase in the tested nanoparticle concentrations to 6 μg/mL; at concentrations over 6 μg/mL, the specific growth rate was almost zero, highlighting the higher toxicity of the nanoparticles on the bacterium ( Table 2). The specific growth rate was decreased from 0.43 h −1 in the control culture to 0.10 h −1 in the culture exposed to 6 μg/mL AgNPs. In terms of CFU, as the concentration of nanoparticles increased in Muller Hinton agar plates, there was a decrease in the number of growing colonies that were absent starting from concentrations ≥12 μg/mL, indicating a concentration-dependent antibacterial activity of AgNPs ( Figure 6).    A time-kill assay revealed that the effect of nanoparticles started after 90 min of incubation at different MIC concentrations. Interestingly, at 1 2 MIC concentration, the Log 10 (CFU/mL) was decreased by 10% compared to the control culture (without AgNPs). However, an almost 10 3 -fold reduction in CFU/mL of S. aureus was noticed after 120 min of cultivation at MIC and 2 MIC concentrations of AgNPs, which indicated a bactericidal effect of the nanoparticles; it caused the killing of the bacterium after 240 min of cultivation (Figure 7). A time-kill assay revealed that the effect of nanoparticles started after 90 min of incubation at different MIC concentrations. Interestingly, at ½ MIC concentration, the Log10 (CFU/mL) was decreased by 10% compared to the control culture (without AgNPs). However, an almost 10 3 -fold reduction in CFU/mL of S. aureus was noticed after 120 min of cultivation at MIC and 2 MIC concentrations of AgNPs, which indicated a bactericidal effect of the nanoparticles; it caused the killing of the bacterium after 240 min of cultivation (Figure 7). It is worth noting that the promising antibacterial activity of AgNPs was attributed to their ability to destroy cell membranes, cause intracellular damage, alter genetic material, and cause oxidative stress in the bacterial cell [94]. Due to their size, nanoparticles have a large surface area that enables them to attach to the cell wall, penetrate the cell, and It is worth noting that the promising antibacterial activity of AgNPs was attributed to their ability to destroy cell membranes, cause intracellular damage, alter genetic material, and cause oxidative stress in the bacterial cell [94]. Due to their size, nanoparticles have a large surface area that enables them to attach to the cell wall, penetrate the cell, and cause a disturbance in the membrane permeability, leading to leakage of the cell content [95,96]. Interestingly, the capping of the nanoparticles with moieties of the lipophilic hypericin, hyperforin, and adhyperforin might facilitate their diffusion across the hydrophobic cell membrane of the bacterial cells; meanwhile, the presence of moieties from the flavonoid and phenols such as rutin and quercitrin surrounding their surfaces could increase their capacity to form complexes with extracellular and soluble proteins and with the cell wall [97]. These chemical constituents were detected among others in the extract of the St. John's wort in a previous report [46]. Moreover, AgNPs can bind to sulfur and phosphorus-containing proteins leading to protein and nucleic acids inactivation [98]. Furthermore, Ag+ ions, released through the oxidation dissolution process, alter the respiratory chain and facilitate the generation of reactive oxygen species (ROS) via their interaction with thiol groups of various enzymes and proteins, thus causing cell death through the inactivation of DNA replication, ATP production, and the activation of the apoptosis pathway [99][100][101].
The effect of the AgNPs on the fibroblast cell migration was examined by the wound migration (contraction) method. The nanoparticles at concentrations of 1-5 µg/mL caused a 52.2-55.5% contraction in the wound distance compared to 67.5% wound closure in untreated fibroblast cells (control) after 24 h of treatment ( Figure 8). However, 31.6% of the wound distance was contracted at 10 µg/mL of AgNPs, a concentration around the IC 50 of the nanoparticle to the fibroblast cell line (data not shown); the decrease in the wound closure at this concentration compared to other tested doses was due to the cytotoxic effect of the nanoparticles on the tested fibroblast cells. In fact, wound migration was non-significantly inhibited when fibroblast cells were treated with 1-5 μg/mL AgNPs (inhibition 19-23% compared to control), but it was significantly inhibited at 10 μg/mL (inhibition 46.8%, p < 0.5) (Figure 9). Fibroblast cells play a fundamental role in body homeostasis through the secretion of growth factors, the release of matrix metalloproteinases, collagen expression, and facilitating contraction of healing wounds [102]. Some studies had reported that ionization of AgNPs by reacting with free oxygen produces a reactive Ag which might regulate the transcriptional factor FOXO1 that stimulates the wound healing molecule, TGF-β1 [89]. Moreover, AgNPs were able to promote fibroblast differentiation and wound contraction in ex vivo systems using rodent models; the ability of these nanoparticles to exert their wound healing effect is through the modulation of inflammatory cytokines [103][104][105]. In fact, wound migration was non-significantly inhibited when fibroblast cells were treated with 1-5 µg/mL AgNPs (inhibition 19-23% compared to control), but it was significantly inhibited at 10 µg/mL (inhibition 46.8%, p < 0.5) (Figure 9). Fibroblast cells play a fundamental role in body homeostasis through the secretion of growth factors, the release of matrix metalloproteinases, collagen expression, and facilitating contraction of healing wounds [102]. Some studies had reported that ionization of AgNPs by reacting with free oxygen produces a reactive Ag which might regulate the transcriptional factor FOXO1 that stimulates the wound healing molecule, TGF-β1 [89]. Moreover, AgNPs were able to promote fibroblast differentiation and wound contraction in ex vivo systems using rodent models; the ability of these nanoparticles to exert their wound healing effect is through the modulation of inflammatory cytokines [103][104][105].
lease of matrix metalloproteinases, collagen expression, and facilitating contraction of healing wounds [102]. Some studies had reported that ionization of AgNPs by reacting with free oxygen produces a reactive Ag which might regulate the transcriptional factor FOXO1 that stimulates the wound healing molecule, TGF-β1 [89]. Moreover, AgNPs were able to promote fibroblast differentiation and wound contraction in ex vivo systems using rodent models; the ability of these nanoparticles to exert their wound healing effect is through the modulation of inflammatory cytokines [103][104][105]. On the contrary, in the current study, it was noticed that the nanoparticles at low concentrations (<IC50) caused an inhibition in fibroblast cell migration and wound contraction; a finding that coincided with the reported effect of the AgNPs in the negative modulation of extracellular matrix molecules (ECM) and laminin deposition, the imbalanced expression of integrins, the impaired reorganization or F-actin cytoskeleton, and decreased human fibroblast migration [102]. Similarly, it was reported that AgNPs-based dressings were unable to reestablish normal skin formation, as they did not cause fibroblast and keratinocyte proliferation [106]. However, Curcuma longa capped AgNPs coating cotton fabrics were effective in wound healing in the fibroblast (L929) cell lines in vitro [13], though such activity was lower than that of untreated control cells. These results On the contrary, in the current study, it was noticed that the nanoparticles at low concentrations (<IC50) caused an inhibition in fibroblast cell migration and wound contraction; a finding that coincided with the reported effect of the AgNPs in the negative modulation of extracellular matrix molecules (ECM) and laminin deposition, the imbalanced expression of integrins, the impaired reorganization or F-actin cytoskeleton, and decreased human fibroblast migration [102]. Similarly, it was reported that AgNPs-based dressings were unable to reestablish normal skin formation, as they did not cause fibroblast and keratinocyte proliferation [106]. However, Curcuma longa capped AgNPs coating cotton fabrics were effective in wound healing in the fibroblast (L929) cell lines in vitro [13], though such activity was lower than that of untreated control cells. These results revealed that the ability of AgNPs in promoting wound healing depends on the test system employed, in vivo or in vitro, as well as the presence of bio-factors such as growth factors and cytokines.
Altogether, smaller AgNPs than previously prepared [46] were synthesized to get a large surface-to-volume ratio. They were functionalized with moieties from H. perforatum aqueous extract that influence NPs' surface charge, hydrophobicity, and/or solubility, thus influencing the particles' structure, size, shape, morphology, degree of aggregation, particle dissolution, ion release, and finally the potential bioactivity. More studies are required in order to confirm the potential role of AgNPs in the wound healing process, and their health impact should be critically evaluated.

Conclusions
During the current study, the aqueous extract of the St. John's wort plant was used to synthesize silver nanoparticles as an environmentally friendly, fast, and clean method. The synthesized AgNPs were characterized by UV-VIS, DLS, zeta potential, FTIR, XRD, SEM, TEM, TGA, and NTA. The resulting AgNPs were spherical and monodisperse, with face-centered cubic crystal structures, and 20-40 nm in size. FTIR revealed the capping of AgNPs with functional groups of St. John's wort extract. The green synthesized nanoparticles exhibited a broad spectral antibacterial activity with Gram-positive test strains as the most susceptible group. The presence of the plant functional group decorating the nanoparticles' surfaces might facilitate their binding to cellular targets and exert their activities. Their bactericidal effect on S. aureus was noticed after 120 min of cultivation at MIC and 2 MIC concentrations; the bacterial specific growth rate significantly declined as the concentration of the tested nanoparticles increased up to 1 2 MIC, and it was zero at MIC and 2 MIC. Furthermore, the wound migration (closure) did not affect AgNPs treatments up to 10 µg/mL.

Data Availability Statement:
The data presented in this study are available on request from the corresponding author.