Rare-Earth-Metal (Nd3+, Ce3+ and Gd3+)-Doped CaF2: Nanoparticles for Multimodal Imaging in Biomedical Applications

Here, we describe the synthesis of a novel type of rare-earth-doped nanoparticles (NPs) for multimodal imaging, by combining the rare-earth elements Ce, Gd and Nd in a crystalline host lattice consisting of CaF2 (CaF2: Ce, Gd, Nd). CaF2: Ce, Gd, Nd NPs are small (15–20 nm), of uniform shape and size distribution, and show good biocompatibility and low immunogenicity in vitro. In addition, CaF2: Ce, Gd, Nd NPs possess excellent optical properties. CaF2: Ce, Gd, Nd NPs produce downconversion emissions in the second near-infrared window (NIR-II, 1000–1700 nm) under 808 nm excitation, with a strong emission peak at 1056 nm. Excitation in the first near- infrared window (NIR-I, 700–900 nm) has the advantage of deeper tissue penetration power and reduced autofluorescence, compared to visible light. Thus, CaF2: Ce, Gd, Nd NPs are ideally suited for in vivo fluorescence imaging. In addition, the presence of Gd3+ makes the NPs intrinsically monitorable by magnetic resonance imaging (MRI). Moreover, next to fluorescence and MR imaging, our results show that CaF2: Ce, Gd, Nd NPs can be used as imaging probes for photoacoustic imaging (PAI) in vitro. Therefore, due to their biocompatibility and suitability as multimodal imaging probes, CaF2: Ce, Gd, Nd NPs exhibit great potential as a traceable imaging agent in biomedical applications.


Introduction
Imaging holds a crucial role in the diagnosis of a variety of diseases such as cancer. Early-stage disease diagnosis is important to maximize treatment effects, and to personalize treatments based on the patient's individual variability and medical profile. Molecular imaging techniques provide comprehensive anatomical, physiological and functional information on disease detection and the monitoring of treatment responses. The most commonly used diagnostic imaging methods during the past few decades in the medical field include MRI, X-ray computed tomography (CT) [1], positron emission tomography (PET) [2,3], single-photon emission tomography (SPECT) [4], optical fluorescent light imaging (FLI) and photoacoustic imaging (PAI) [5]. Due to differences in their detection methods, spatiotemporal resolution, sensitivity and probe types, the diagnostic information obtained is divergent. Both PET and SPECT use γ rays to detect the in vivo distribution of radioactive tracers to obtain information on biological functions. They have the disadvantages of low spatial resolution, radiation risks and high costs [6][7][8][9][10]. Optical imaging uses visible light and near-infrared probes with different spectral characteristics for molecular and cellular detection but faces several limitations, such as photobleaching, low tissue penetration power, low spatial resolution and autofluorescence [11][12][13][14][15]. While CT, MRI and PAI can the material in our strategy to construct an imaging mode combining NIR-II imaging with complementary MRI and PAI.
In this study, we used a hydrothermal method to synthesize a citric acid-terminated nanomaterial with CaF 2 as the matrix, co-doped with the three lanthanide elements-Ce, Gd and Nd. The resulting CaF 2 : Ce, Gd, Nd NPs had a small particle size, were stable in the presence of serum and showed excellent luminous intensity at 1056 nm. In vitro cell culture experiments demonstrated that CaF 2 : Ce, Gd, Nd NPs did not induce cellular cytotoxicity and were readily taken up by human breast cancer cells. In addition, the NPs showed low immunogenicity when cultured with antigen-presenting cells, and thus the NPs can be used as a potential imaging probe in vivo. Importantly, CaF 2 : Ce, Gd, Nd NPs are multimodal and can be detected by NIR-II/PA/MR imaging and therefore provide significant advantages in disease diagnosis. In summary, CaF 2 : Ce, Gd, Nd NPs represent a novel optical material that can be widely used in the field of medical imaging.

Materials
The following chemicals were acquired from Sigma-Aldrich ( 2.2. Synthesis of the CaF 2 : Ce, Gd, Nd NPs As previously described [50], we synthesized CaF 2 : Ce, Gd, Nd NPs by a simple hydrothermal method. Briefly, a total of 3.75 mmol of CaCl 2 ·2H 2 O, CeCl 3 ·7H 2 O, GdCl 3 ·6H 2 O and NdCl 3 ·6H 2 O (Ca 0.98−2x Ce x Gd x Nd 0.02 F 2.02+2x , x = 0.15) were dissolved in 7 mL water and stirred for 10 minutes (min) until fully dissolved. Then, potassium citrate solution was added dropwise to the solution and stirred for 30 min. After that, NH 4 F solution was added, and the solution was stirred evenly. The final solution was transferred to a 50 mL Teflon bottle (Baoshishan, China) held in a stainless-steel autoclave, put in an oven (Heraeus, Germany) and maintained at 180 • C for 10 hours (h). Finally, the obtained sample was centrifuged at 2.4× g for 20 min and washed three times with water followed by ethanol (99%). The samples were dried in a freeze-dryer (Martin Christ, Osterode, Germany).
The synthesis of CaF 2 : Nd NPs, CaF 2 : Ce, Nd NPs, CaF 2 : Ce, Gd NPs was similar to that of CaF 2 : Ce, Gd, Nd NPs. The doped concentration of Nd 3+ was maintained at 0.02, and Ce 3+ and Gd 3+ were maintained at 0.15.

Characterization
X-ray diffraction (XRD) analysis of the CaF 2 : Ce, Gd, Nd NPs was performed by a Panalytical X'pert PRO (Malvern Panalytical, Malvern, UK) operating at a tube voltage of 40 kV and a tube current of 40 mA. The diffraction patterns were acquired using Cu Kα radiation (λ = 1.5405 Å) at a scanning rate of 6.0 • /min in the 2θ range of 10 • ≤ 2θ ≤ 70 • .
The hydrodynamic size and Zeta potential were obtained from CaF 2 : Ce, Gd, Nd NPs solution (1 mg/mL) using a Malvern ZetaSizer 2000 (Malvern, UK). The analysis software applications were Zetasizer Software (Version 7.13) and GraphPad Prism 8.
IRSpirit FTIR spectrophotometer (Shimadzu, Kyoto, Japan) was used to measure the Fourier transform infrared (FTIR) spectra of CaF 2 : Ce, Gd, Nd NPs powder. The spectra were captured in the IRSpirit-TOAPC0027956 mode with a wavenumber range of 600-4000 cm −1 and a resolution of 4 cm −1 . IR Pilot and Origin 8.5 were used for analysis.
The size and morphology of CaF 2 : Ce, Gd, Nd NPs were examined using a Tecnai 12 Twin transmission electron microscope (TEM) (FEI Company, Hillsboro, OR, USA) outfitted with a OneView Camera Model 1095 (Gatan, Pleasanton, CA, USA) under 120 kV voltage. TEM samples were prepared by pipetting CaF 2 : Ce, Gd, Nd NPs aqueous solution (1 mg/mL) onto the surface of the copper grid.
CaF 2 : Ce, Gd, Nd NPs were mounted on scanning electron microscopy (SEM) specimen stubs. Then, an Apreo S LoVac SEM (Thermo Scientific, Waltham, MA, USA) equipped with an UltraDry energy-dispersive X-ray spectroscopy (EDS) detector (Thermo Scientific, Waltham, MA, USA) was utilized to analyze the sample. The measurement conditions were: 1500× magnification with 30 kV and 51 nA.
A Quantum Design Versalab physical property measurement system with VSM option (Quantum Design, San Diego, CA, USA) was employed to quantify the vibrating-sample magnetometry (VSM) of CaF 2 : Ce, Gd, Nd NPs (4.51 mg).
The emission spectra of CaF 2 :Ce, Gd, Nd NPs powder was recorded using Fluorolog ® -3 with FluoEssence TM (Horiba, Kyoto, Japan) equipped with a diode laser as the excitation light source, and the emission spectra of CaF 2 : Nd NPs (powder) and CaF 2 : Ce, Nd NPs (powder) were recorded using an Edinburgh FLS920 fluorescence spectrometer (Edinburgh Instruments, Edinburgh, UK) with an 808 nm NIR diode laser (300 mW). The absorption spectra of CaF 2 : Ce, Gd, Nd NPs (10 mg/mL) and CaF 2 : Ce, Gd NPs (10 mg/mL) were obtained by SpectraMax ® iD3 Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA, USA). Analysis tool was SoftMax Pro ® 7 Software.

Stability of CaF 2 :Ce, Gd, Nd NPs
To assess the stability of CaF 2 : Ce, Gd, Nd NPs in physiologically relevant buffers, we dissolved CaF 2 : Ce, Gd, Nd NPs at a concentration of 0.2 mg/mL in 50% FCS solution. The samples were kept in a shaker at 37 • C, and we used a Malvern ZetaSizer 2000 (Malvern, UK) to measure the size and zeta potential at 0 h, 1 d, 2 d, 3 d, 4 d, 5 d, 6 d and 7 d. Data analysis used Zetasizer Software (Version 7.13) and GraphPad Prism 8.

Hemolysis of CaF 2 : Ce, Gd, Nd NPs
Briefly, a total of 100 µL of fresh blood was collected from the tail vein of BALB/c mice using a vacuum blood collection tube. Ca 2+ /Mg 2+ -free PBS was used to dilute the blood 50 times. To extract red blood cells, the blood dilutions were centrifuged at 0.9× g for 15 min at 4 • C. The supernatant was removed, and two groups were chosen randomly as negative and positive controls. The negative control group was resuspended with 500 µL of saline, whereas the positive control group was resuspended with 500 µL of 1% Triton X-100 (v/v). For the experimental group, blood cells were resuspended with peripheral blood mononuclear cells (PBMCs)/well, kept in a 37 • C incubator and then incubated with various CaF 2 : Ce, Gd, Nd NPs concentrations (0-125 µg/mL) for 24 h, 48 h and 72 h. Afterwards, the medium was removed and 100 µL of fresh medium and 20 µL of MTS reagent were added to each well as directed by the manufacturer's instructions. The cells were then cultured for 1.5 h at 37 • C in an incubator. The absorbance (OD) was measured using Microplate reader (Molecular Devices, San Jose, CA, USA) at 490 nm. The ratio to the untreated control group was used to assess cell viability. Data are presented as mean ± SD.
2.7. Uptake of CaF 2 : Ce, Gd, Nd NPs Qualitative uptake of CaF 2 : Ce, Gd, Nd NPs in 4T1 cells was determined by confocal microscopy. 4T1 cells were seeded at 2 × 10 4 /well on 13 mm 3 circular coverslips in 24-well plates. After 24 h, 4T1 cells were co-incubated with 250 µg/mL of CaF 2 : Ce, Gd, Nd NPs medium at 37 • C for 1, 4, 24 and 48 h. Cells treated with medium without NPs served as a control group. At the end of the incubation time, the cells were washed 5 times with PBS to remove the unabsorbed NPs. At room temperature, the cells were fixed with 4% paraformaldehyde for 15 min, washed twice with PBS, treated with 0.1% Triton PBS for 10 min and washed 3 times with PBS. Finally, cell nuclei were labeled with DAPI for 5 min. After washing with PBS, coverslips were sealed on slides with fluorCare sealer. The slides were imaged using a SP8 LIGHTNING Confocal Microscope (Leica Biosystems Nussloch GmbH, Germany) and analyzed by LAS X (Leica Application Suite X) software.

In Vitro Dendritic Cell (DC) Activation Study
In order to evaluate the effect of CaF 2 : Ce, Gd, Nd NPs on immune cells, we used flow cytometry to assess the expression of DC maturation/activation markers. Briefly, murine D1 DCs and 125 µg/mL CaF 2 : Ce, Gd, Nd NPs were co-cultured in a 96-well plate for 24 h in a 37 • C incubator. PBS/EDTA (Sigma-Aldrich, St. Louis, MO, USA) was used to detach the cells, which were then washed with FACS buffer and stained with anti-CD40-APC (Biolegend, San Diego, CA, USA) and anti-CD86-FITC (eBioscience, San Diego, CA, USA) antibodies. The cells were washed and resuspended in 100 µL FACS buffer after 30 min. A LSR-II cytometer (BD Biosciences, Franklin Lakes, NJ, USA) was used to measure the samples, and FlowJo (version 10) was utilized to analyze the data.
2.9. Multimodal Imaging Properties of CaF 2 : Ce, Gd, Nd NPs 2.9.1. NIR-II imaging We employed an in vivo NIR-II optical imaging system (Kaer Labs, Nantes, France) to evaluate the NIR-II imaging capabilities of our NPs. CaF 2 : Ce, Gd, Nd NPs were suspended in ddH 2 O at 2 mg/mL, while the InGaAs camera was chilled to −20 • C at mid-gain setup. We obtained photos at various wavelengths using an 808 nm laser excitation at 50 mW/cm 2 . Images were recorded with the KIS NIR-II system.

Photoacoustic Imaging
The PA and B-mode ultrasound images were acquired using Vevo LAZR-X (FUJIFILM VisualSonics, Toronto, ON, Canada), and a MX550D transducer was utilized. CaF 2 : Ce, Gd, Nd NP solution was injected into an 0.5% agarose gel. The experiment was carried out with the center transmit of 40 MHz and the axial resolution of 40 µm. Vevo LAB 5.5.0 was used to analyze the data.

MRI Studies
MRI studies were carried out using a 7T Bruker BioSpec (Ettlingen, Germany), and ParaVision 360 (Version 2.0. pl.1) software was used to analyze attenuation images. CaF 2 : Ce, Gd, Nd NPs were dissolved in 0.5% agarose solution, and the concentrations were 0 mg/mL, 1 mg/mL, 2.5 mg/mL, 5 mg/mL, 7.5 mg/mL and 10 mg/mL. The microwave oven was used to heat the solutions and create the gel sample. The measurement settings were as follows: 500/11 ms for the repetition time/echo time (TR/TE), 4 for the number of excitations (NEX), 128 × 128 for the matrix size (MTX), 40 × 40 mm 2 for the field of view (FoV) and 1.20 mm for the slice thickness (SL). To investigate the MRI characteristics of NPs under biological conditions, we fixed C57BL/6J mouse cadavers with a tiny animal coil after subcutaneously injecting 100 µL of CaF 2 : Ce, Gd, Nd NPs (10 mg/mL) and took MR images before and after the injection. The measurement conditions were TR/TE = 10/2.8 ms, FoV = 40 × 40 mm 2 , matrix = 256 × 256.

Results and Discussion
To explore whether our strategy of synthesizing a multimodal citric acid-terminated nanomaterial with CaF 2 as the matrix, co-doped with the three lanthanide elements-Ce, Gd and Nd-was feasible, we adapted a previously published hydrothermal method to obtain Yb 3+ /Tm 3+ -doped MF 2 (M = Ca, Sr) colloids to synthesize CaF 2 : Ce, Gd, Nd. Briefly, Ca 2+ , Ce 3+ , Gd 3+ , Nd 3+ were dissolved in water. Next, solutions of potassium citrate tribasic monohydrate and NH 4 F were added in turn to obtain the final solution. The resulting solution was heated in an oven (Heraeus, Germany) at 180 • C for 10 h to synthesize NPs. The obtained sample was centrifuged, washed and freeze-dried (Scheme 1). The physicochemical properties of the synthesized NPs were carefully characterized.

MRI Studies
MRI studies were carried out using a 7T Bruker BioSpec (Ettlingen, Germany), and ParaVision 360 (Version 2.0. pl.1) software was used to analyze attenuation images. CaF2: Ce, Gd, Nd NPs were dissolved in 0.5% agarose solution, and the concentrations were 0 mg/mL, 1 mg/mL, 2.5 mg/mL, 5 mg/mL, 7.5 mg/mL and 10 mg/mL. The microwave oven was used to heat the solutions and create the gel sample. The measurement settings were as follows: 500/11 ms for the repetition time/echo time (TR/TE), 4 for the number of excitations (NEX), 128 × 128 for the matrix size (MTX), 40 × 40 mm 2 for the field of view (FoV) and 1.20 mm for the slice thickness (SL). To investigate the MRI characteristics of NPs under biological conditions, we fixed C57BL/6J mouse cadavers with a tiny animal coil after subcutaneously injecting 100 μL of CaF2: Ce, Gd, Nd NPs (10 mg/mL) and took MR images before and after the injection. The measurement conditions were TR/TE = 10/2.8 ms, FoV = 40 × 40 mm 2 , matrix = 256 × 256.

Results and Discussion
To explore whether our strategy of synthesizing a multimodal citric acid-terminated nanomaterial with CaF2 as the matrix, co-doped with the three lanthanide elements-Ce, Gd and Nd-was feasible, we adapted a previously published hydrothermal method to obtain Yb 3+ /Tm 3+ -doped MF2 (M = Ca, Sr) colloids to synthesize CaF2: Ce, Gd, Nd. Briefly, Ca 2+ , Ce 3+ , Gd 3+ , Nd 3+ were dissolved in water. Next, solutions of potassium citrate tribasic monohydrate and NH4F were added in turn to obtain the final solution. The resulting solution was heated in an oven (Heraeus, Germany) at 180 °C for 10 h to synthesize NPs. The obtained sample was centrifuged, washed and freeze-dried (Scheme 1). The physicochemical properties of the synthesized NPs were carefully characterized. First, to confirm that the main matrix of the synthesized NPs consisted of CaF2, we performed an XRD analysis (Figure 1a). Through XRD pattern analysis, it can be seen that the main diffraction peaks of CaF2: Ce, Gd, Nd powder correspond to the CaF2 crystal standard card, and the addition of Ce 3+ , Gd 3+ , Nd 3+ did not change the CaF2 cubic phase structure but only resulted in a slight shift of diffraction peaks (Figure 1a). This is because the trivalent lanthanide ions replaced Ca 2+ , which resulted in Ca vacancies and exhibited First, to confirm that the main matrix of the synthesized NPs consisted of CaF 2 , we performed an XRD analysis (Figure 1a). Through XRD pattern analysis, it can be seen that the main diffraction peaks of CaF 2 : Ce, Gd, Nd powder correspond to the CaF 2 crystal standard card, and the addition of Ce 3+ , Gd 3+ , Nd 3+ did not change the CaF 2 cubic phase structure but only resulted in a slight shift of diffraction peaks (Figure 1a). This is because the trivalent lanthanide ions replaced Ca 2+ , which resulted in Ca vacancies and exhibited lattice defects. Moreover, due to the differences in ionic radius and valence, the excess of positive charge needed more F − to compensate, which changed the bond length and reduced the symmetry of the crystal [51]. In addition, a small peak appeared at about 30 • , which has been demonstrated to correspond to the doping of rare-earth elements [52,53]. This also confirmed the successful doping of Ce 3+ , Gd 3+ , Nd 3+ into the CaF 2 crystal. DLS data showed that the NPs had a relatively stable particle size of 29.53 ± 0.16 nm in water. Zeta potential measurement showed that the NPs had a negative surface charge of −16.6 ± 0.75 mV. This is because sodium citrate was used as a complexing agent during NPs synthesis, and as a result, the NPs surface harbors carboxyl groups. The carboxyl group of citrate is negatively charged in water, and the electrostatic repulsion is the reason why CaF 2 : Ce, Gd, Nd NPs are stable in solution. To examine the stability and shelf-life of CaF 2 : Ce, Gd, Nd NPs, we repeated the DLS measurement after storing the NPs for 4 months at room temperature (Figure 1b). There was no significant change in the particle size and zeta potential of the NPs, suggesting that CaF 2 : Ce, Gd, Nd NPs show excellent shelf-life stability. CaF 2 : Ce, Gd, Nd NPs have two main absorption peaks at 1392 and 1572 cm −1 , which correspond to the sodium citrate bands (Figure 1c). On the other hand, the absorption peaks, indicating that the carboxyl groups were conjugated to the metal ion on the surface of the NPs. Therefore, FTIR effectively proved the presence of citrate anions on the surface of the CaF 2 : Ce, Gd, Nd NPs [54]. In order to study the morphology of CaF 2 : Ce, Gd, Nd NPs more accurately, we performed a TEM analysis (Figure 1d). The TEM results showed that the NPs have a uniform and stable morphology, about 12 nm, which met our expectations. The discrepancy between the DLS and TEM results on particle size is due to the fact that the DLS measures the hydrodynamic diameter, while the TEM measures the hard boundary [55]. Additionally, to further investigate the elemental composition of the CaF 2 : Ce, Gd, Nd NPs, we performed EDS on powdered NPs. In Figure 1e, we can see that the strongest peak corresponds to Ca, the smaller peaks to F, Ce and Gd and the weakest peak to Nd. We did not detect any impurity peaks. This result is consistent with the proportion of doped elements in our synthesis process. In addition, mapping results revealed signals for all these elements, and the distribution is homogeneous (Figure 1f). In summary, these results prove that the rare-earth ions (Ce 3+ , Gd 3+ , Nd 3+ ) have been successfully doped into CaF 2 .
In order to evaluate the optical properties of the NPs, we first measured the absorption spectra of the CaF 2 : Ce, Gd, Nd NPs and CaF 2 : Ce, Gd NPs in aqueous solution at a concentration of 10 mg/mL (Figure 2a). Compared to CaF 2 : Ce, Gd NPs, the CaF 2 : Ce, Gd, Nd NPs showed absorption peaks at 576 nm,734 nm and 795 nm, corresponding to the 4 G 5/2 + 2 G 7/2 , 4 F 7/2 + 4 S 3/2 , and 4 F 5/2 + 2 H 9/2 absorption peaks of Nd 3+ [45,56]. Then, to demonstrate that the doping of Ce increased the luminescence intensity of the NPs, we first tested the luminescence intensity of CaF 2 : Nd and CaF 2 : Ce, Nd under 808 nm laser excitation (300 mW) (Figure 2b). The experimental results demonstrated that the doping of Ce significantly increased the luminescence intensity of the NPs, and this result was consistent with the results of Wang et al. [47] Next, we measured the luminescence intensity of the NP sample under increasing laser powers at an excitation wavelength of 808 nm (Figure 2c). The NPs showed a strong signal in the near-infrared region, with two main emission peaks at 1056 nm and 1340 nm, corresponding to the 4 F 3/2 → 4 I 11/2 and 4 F 3/2 → 4 I 13/2 electronic transitions of Nd 3+ , respectively [57][58][59]. The strongest emission peak was observed at 1056 nm, when the laser power reached 7.7 mW, and an intensity of about 5×10 4 , which proves its unique NIR-II efficiency (Figure 2d). On the other hand, since Nd 3+ is the main emission center of the NPs, under 808nm laser excitation, CaF 2 : Ce, Gd, Nd NPs can effectively reduce the biological tissue damage during the application process, especially the overheating effect caused by the laser. Moreover, the Nd 3+ emission spectra were located in the second near-infrared window (1000-1400 nm), resulting in a deeper penetration depth and smaller autofluorescence effect of the NPs [52]. Based on the above generated data, an NIR-II system was employed to evaluate the NIR-II imaging properties of CaF 2 : Ce, Gd, Nd NPs (Figure 2e). Under 808 nm laser excitation, CaF 2 : Ce, Gd, Nd NPs showed near-infrared emission at 1064 nm. This result is consistent with the 4 F 3/2 → 4 I 11/2 energy transition of Nd 3+ , which proves that the main emission center is Nd 3+ . Thus, our data confirms that CaF 2 : Ce, Gd, Nd NPs are suitable NIR-II imaging probes.
Good colloidal stability is one of the prerequisites for judging whether NPs can be used in biological applications. In order to determine whether CaF 2 : Ce, Gd, Nd NPs have good colloidal stability, the NPs were redispersed in NaCl containing 50% FCS, and then the particles were measured repeatedly (three times) using a Malvern ZetaSizer 2000 (Malvern, UK) at different time periods to determine the particle size. The results showed that there was no significant change in the size and zeta potential of the NPs in 50% FCS (Figure 3a,b). This demonstrates that the NPs exhibit a relatively stable state when 50% FCS was added to NaCl.   ance (λ = 540 nm) of the samples with the positive (1% Triton X-100) and negative (saline) controls [60] (Figure 3c,d). At the highest concentration (1 mg/mL), the hemolysis rate was calculated to be 0.08%, which is negligible. Thus, our results show that CaF2: Ce, Gd, Nd NPs have excellent blood compatibility and can be used for intravenous in vivo imaging. (b) Zeta potential of CaF 2 : Ce, Gd, Nd NPs in 50% FCS; (c) hemolysis of CaF 2 : Ce, Gd, Nd NPs after incubation with red blood cells at multiple concentrations (0-1 mg/mL) for 4 h, with PBS and deionized water serving as negative and positive controls, respectively; (d) hemolysis photo after centrifugation. All data presented the mean values ± SD from three independent trials. By comparing experimental groups to control group, one-way ANOVA was used to determine statistical significance (**** p < 0.0001).
To evaluate the NPs' biocompatibility with blood components, the effect of CaF 2 : Ce, Gd, Nd NPs on red blood cell (RBC) hemolysis was studied. To this end, murine peripheral blood cells were incubated at 37 • C for 4 h with different concentrations of CaF 2 : Ce, Gd, Nd NPs, and the hemolysis rate of the NPs was calculated by comparing the absorbance (λ = 540 nm) of the samples with the positive (1% Triton X-100) and negative (saline) controls [60] (Figure 3c,d). At the highest concentration (1 mg/mL), the hemolysis rate was calculated to be 0.08%, which is negligible. Thus, our results show that CaF 2 : Ce, Gd, Nd NPs have excellent blood compatibility and can be used for intravenous in vivo imaging.
To determine whether the NPs induce cellular toxicity, the effect of CaF 2 : Ce, Gd, Nd NPs on the viability of 4T1 cells was measured by MTS assay (Figure 4). 4T1 is a highly tumorigenic and aggressive breast cancer cell line which grows and spreads metastatically, similar to human breast cancer, and is used as a typical cell model for cancer studies. 4T1 cells were cultured in the presence of different concentrations of CaF 2 : Ce, Gd, Nd NPs for 24 h, 48 h and 72 h. At 24 h, CaF 2 : Ce, Gd, Nd NPs below 1000 µg/mL had no significant effect on the viability of 4T1 cells (p > 0.05). Cell viability was significantly higher at a concentration of 2000 µg/mL (** p < 0.01). Values higher than 100% indicate that the NPs may have a positive effect on cell proliferation [61]. Secondly, CaF 2 : Ce, Gd, Nd NPs contain citric acid groups on the surface, which may participate in the intracellular tricarboxylic acid cycle reaction, resulting in increased cell activity. At 48 h, cell viability was significantly reduced by 15% when the concentration of CaF 2 : Ce, Gd, Nd NPs was 1000 µg/mL compared to the control (* p<0.05). At 72 h, the doses of 500 and 250 µg/mL CaF 2 : Ce, Gd, Nd NPs showed a significant inhibitory trend on 4T1 cells (*** p < 0.001, **** p < 0.0001). There was no difference between the cell survival rates when the concentration was < 125 µg/mL compared to the control group (p > 0.05). As cancer cell lines show biological differences compared to normal, healthy cells, we also tested the effect of CaF 2 : Ce, Gd, Nd NPs (0-125 µg/mL) on the viability of human peripheral blood mononuclear cells (PBMCs). As shown in Figure S1, there was no significant cytotoxic effect of the NPs on cell activity at 24 h and 48 h after NP incubation compared to the control group. Thus, CaF 2 : Ce, Gd, Nd NPs show great promise for biological applications, but further studies are needed to determine the optimal concentration and potential cytotoxic effects.
To determine whether the NPs induce cellular toxicity, the effect of CaF2: Ce, Gd, Nd NPs on the viability of 4T1 cells was measured by MTS assay (Figure 4). 4T1 is a highly tumorigenic and aggressive breast cancer cell line which grows and spreads metastatically, similar to human breast cancer, and is used as a typical cell model for cancer studies. 4T1 cells were cultured in the presence of different concentrations of CaF2: Ce, Gd, Nd NPs for 24 h, 48 h and 72 h. At 24 h, CaF2: Ce, Gd, Nd NPs below 1000 μg/mL had no significant effect on the viability of 4T1 cells (p > 0.05). Cell viability was significantly higher at a concentration of 2000 μg/mL (** p < 0.01). Values higher than 100% indicate that the NPs may have a positive effect on cell proliferation [61]. Secondly, CaF2: Ce, Gd, Nd NPs contain citric acid groups on the surface, which may participate in the intracellular tricarboxylic acid cycle reaction, resulting in increased cell activity. At 48 h, cell viability was significantly reduced by 15% when the concentration of CaF2: Ce, Gd, Nd NPs was 1000 μg/mL compared to the control (* p<0.05). At 72 h, the doses of 500 and 250 μg/mL CaF2: Ce, Gd, Nd NPs showed a significant inhibitory trend on 4T1 cells (*** p < 0.001, **** p < 0.0001). There was no difference between the cell survival rates when the concentration was < 125 μg/mL compared to the control group (p > 0.05). As cancer cell lines show biological differences compared to normal, healthy cells, we also tested the effect of CaF2: Ce, Gd, Nd NPs (0-125 μg/mL) on the viability of human peripheral blood mononuclear cells (PBMCs). As shown in Figure S1, there was no significant cytotoxic effect of the NPs on cell activity at 24 h and 48 h after NP incubation compared to the control group. Thus, CaF2: Ce, Gd, Nd NPs show great promise for biological applications, but further studies are needed to determine the optimal concentration and potential cytotoxic effects. mean values ± SD from three independent experiments. Statistical significance was calculated using two-way ANOVA, by comparing experimental groups to control group (* p< 0.05, ** p< 0.01, *** p < 0.001 and **** p < 0.0001).
We further investigated the uptake potential of CaF2: Ce, Gd, Nd NPs by murine breast cancer cells. As demonstrated in Figure 2a, optical characteristics of CaF2: Ce, Gd, Nd NPs overlap with the excitation and emission spectra of commonly employed dyes in flow cytometry and microscopy applications, such as Alexa Fluor 568. Thus, we reasoned ± SD from three independent experiments. Statistical significance was calculated using two-way ANOVA, by comparing experimental groups to control group (* p< 0.05, ** p< 0.01, *** p < 0.001 and **** p < 0.0001).
We further investigated the uptake potential of CaF 2 : Ce, Gd, Nd NPs by murine breast cancer cells. As demonstrated in Figure 2a, optical characteristics of CaF 2 : Ce, Gd, Nd NPs overlap with the excitation and emission spectra of commonly employed dyes in flow cytometry and microscopy applications, such as Alexa Fluor 568. Thus, we reasoned that our NPs might be inherently monitorable by confocal microscopy. In order to determine the NP uptake rate and intracellular localization of NPs in 4T1 cells, we performed a confocal analysis ( Figure 5). To this end, 4T1 cells were incubated with 250 µg/mL CaF 2 : Ce, Gd, Nd NPs (red) at 37 • C for 1 h, 4 h, 24 h and 48 h, followed by co-labeling with DAPI (blue) to stain the nucleus and phalloidin (green) to stain the actin cytoskeleton. Visual inspection confirmed that CaF 2 : Ce, Gd, Nd NPs could be effectively visualized as distinct dots by confocal microscopy. After 1 h co-incubation, few NPs were found attached to the cell membrane. With increasing incubation time, more red fluorescent dots were found in the cytoplasm, indicating that more NPs entered the cells ( Figure 5). Based on the above results, we conclude that CaF 2 : Ce, Gd, Nd NPs were effectively taken up by 4T1 cancer cells.
Visual inspection confirmed that CaF2: Ce, Gd, Nd NPs could be effectively visualized as distinct dots by confocal microscopy. After 1 h co-incubation, few NPs were found attached to the cell membrane. With increasing incubation time, more red fluorescent dots were found in the cytoplasm, indicating that more NPs entered the cells ( Figure 5). Based on the above results, we conclude that CaF2: Ce, Gd, Nd NPs were effectively taken up by 4T1 cancer cells. Next, we determined whether CaF2: Ce, Gd, Nd NPs, without further modification, can activate the immune system, which could cause adverse side effects in vivo. Previous studies have shown that when immature dendritic cells (DCs) encounter various activation stimuli, they will mature and increase the expression of costimulatory markers on their surface [62][63][64]. Therefore, we cultured murine immature D1DCs in the presence of CaF2: Ce, Gd, Nd NPs for 24 h and assessed the expression of the DC costimulatory receptors CD86 and CD40 by flow cytometry. As positive control for DC maturation, we included D1 cells that were treated with LPS. After duplicate exclusion, the expression of Next, we determined whether CaF 2 : Ce, Gd, Nd NPs, without further modification, can activate the immune system, which could cause adverse side effects in vivo. Previous studies have shown that when immature dendritic cells (DCs) encounter various activation stimuli, they will mature and increase the expression of costimulatory markers on their surface [62][63][64]. Therefore, we cultured murine immature D1DCs in the presence of CaF 2 : Ce, Gd, Nd NPs for 24 h and assessed the expression of the DC costimulatory receptors CD86 and CD40 by flow cytometry. As positive control for DC maturation, we included D1 cells that were treated with LPS. After duplicate exclusion, the expression of CD86 and CD40 was analyzed on single D1 cells (Figure 6a). In the non-treated control group, 8% of the cells were double positive for CD86 and CD40 (Figure 6a), and the expression increased to 84% when the cells were treated with LPS ( Figure 6b). When D1 cells were treated with a high concentration of CaF 2 : Ce, Gd, Nd NPs (125 µg/mL), 7% of the cells co-expressed CD86 and CD40 (Figure 6c), similar to the levels in the controls group, and in a negative controls group, where Au NPs were added that are known to be non-immunogenic (Figure 6d). In summary, these data indicate that CaF 2 : Ce, Gd, Nd NPs are inert and do not induce immune activation.
pression increased to 84% when the cells were treated with LPS (Figure 6b). When D1 cells were treated with a high concentration of CaF2: Ce, Gd, Nd NPs (125 μg/mL), 7% of the cells co-expressed CD86 and CD40 (Figure 6c), similar to the levels in the controls group, and in a negative controls group, where Au NPs were added that are known to be nonimmunogenic (Figure 6d). In summary, these data indicate that CaF2: Ce, Gd, Nd NPs are inert and do not induce immune activation. Yang et al. have shown that rare-earth-doped particles can be employed as PAI agents [65]. Furthermore, since PAI is an imaging method that combines light excitation and ultrasound technology, the multiple absorption peaks of rare-earth NPs provide an opportunity as a PAI contrast agent. In order to determine whether CaF2: Ce, Gd, Nd NPs are suitable for ultrasound imaging, we placed the NPs into an agarose phantom, mimicking biological tissue, for imaging by PA (Figure 7). In this gel phantom, the NPs showed PA signals at 808 nm wavelength, which was stronger than in the control agarose without NPs, but slightly dimmer than in the ICG group, a dye that is well suited for PAI. When the NPs were irradiated by the laser, part of the light energy was absorbed and converted into heat energy, which caused thermoelastic expansion and generated the PA signal. It effectively proves that CaF2: Ce, Gd, Nd NPs have the ability to serve as NIR-II and PA probes. Yang et al. have shown that rare-earth-doped particles can be employed as PAI agents [65]. Furthermore, since PAI is an imaging method that combines light excitation and ultrasound technology, the multiple absorption peaks of rare-earth NPs provide an opportunity as a PAI contrast agent. In order to determine whether CaF 2 : Ce, Gd, Nd NPs are suitable for ultrasound imaging, we placed the NPs into an agarose phantom, mimicking biological tissue, for imaging by PA (Figure 7). In this gel phantom, the NPs showed PA signals at 808 nm wavelength, which was stronger than in the control agarose without NPs, but slightly dimmer than in the ICG group, a dye that is well suited for PAI. When the NPs were irradiated by the laser, part of the light energy was absorbed and converted into heat energy, which caused thermoelastic expansion and generated the PA signal. It effectively proves that CaF 2 : Ce, Gd, Nd NPs have the ability to serve as NIR-II and PA probes. Since Gd 3+ chelating material is a common "positive" clinical MRI contrast agent, we inferred that CaF2: Ce, Gd, Nd NPs might be suitable as imaging probes for MRI. The magnetic properties of CaF2: Ce, Gd, Nd NPs were first verified using VSM. At room temperature (300 K) and an applied magnetic field of 1.5 T, we noticed that the NPs enhance Since Gd 3+ chelating material is a common "positive" clinical MRI contrast agent, we inferred that CaF 2 : Ce, Gd, Nd NPs might be suitable as imaging probes for MRI. The magnetic properties of CaF 2 : Ce, Gd, Nd NPs were first verified using VSM. At room temperature (300 K) and an applied magnetic field of 1.5 T, we noticed that the NPs enhance their magnetic properties as the magnetic field increases, showing a typical paramagnetic behavior consistent with the magnetic characteristics of Gd ions (Figure 8a). The diamagnetic contribution was calculated to be 0.0032 Am 2 /kg [66,67]. In order to prove the hypothesis that CaF 2 : Ce, Gd, Nd NPs can be used as a MRI probe, we mixed CaF 2 : Ce, Gd, Nd NPs with agarose gel and performed an MRI measurement. Figure 8b shows that as the concentration of NPs increased, the MRI image became brighter. Analysis of the data showed that due to the increase in the concentration of CaF 2 : Ce, Gd, Nd NPs, the paramagnetic strength increased, which led to an increase in the longitudinal relaxation time (T1), resulting in a bright image on the T1 weighted imaging (Figure 8c,d). To better investigate the potential biological applications of NPs, we injected NPs subcutaneously into mouse cadavers and found a clear signal at the injection site (Figure 8e). These results indicate that CaF 2 : Ce, Gd, Nd NPs can be used as MRI contrast agents.

Conclusions
In summary, we doped Ce 3+ , Gd 3+ and Nd 3+ into CaF2 crystals through a simple hydrothermal process, resulting in the synthesis of CaF2: Ce, Gd, Nd NPs suitable for multimodal imaging. The synthesized NPs were highly pure, and showed low toxicity, good biocompatibility and no immunogenicity. CaF2: Ce, Gd, Nd NPs themselves exhibited 3+ 3+

Conclusions
In summary, we doped Ce 3+ , Gd 3+ and Nd 3+ into CaF 2 crystals through a simple hydrothermal process, resulting in the synthesis of CaF 2 : Ce, Gd, Nd NPs suitable for multimodal imaging. The synthesized NPs were highly pure, and showed low toxicity, good biocompatibility and no immunogenicity. CaF 2 : Ce, Gd, Nd NPs themselves exhibited dual modes because Ce 3+ and Nd 3+ dopants contribute to NIR-II and PAI, and the presence of Gd 3+ shows a high-contrast T1-enhancing effect for MRI. Therefore, CaF 2 : Ce, Gd, Nd NPs may be an informative NIR-II/PA/MR multimodal probe for clinical diagnosis. This research also laid the foundation for the use of CaF 2 : Ce, Gd, Nd NPs for biological imaging of cells and deep tissues.
Supplementary Materials: The following supporting information can be downloaded at: https: //www.mdpi.com/article/10.3390/pharmaceutics14122796/s1, Figure S1: Cell viability. PBMCs were treated with CaF 2 : Ce, Gd, Nd NPs at varying concentrations (0-125 µg/mL) on PBMCs for 24 h and 48 h. Data represent the mean values ± SD from three independent experiments. Statistical significance was calculated using two-way ANOVA, by comparing experimental groups to control group.