Development of an Orodispersible Film Containing Stabilized Influenza Vaccine

Most influenza vaccines are administered via injection, which is considered as user-unfriendly. Vaccination via oral cavity using an orodispersible film (ODF) might be a promising alternative. To maintain the antigenicity of the vaccine during preparation and subsequent storage of these ODFs, sugars such as trehalose and pullulan can be employed as stabilizing excipients for the antigens. In this study, first, β-galactosidase was used as a model antigen. Solutions containing β-galactosidase and sugar (trehalose or trehalose/pullulan blends) were pipetted onto plain ODFs and then either air- or vacuum-dried. Subsequently, sugar ratios yielding the highest β-galactosidase stability were used to prepare ODFs containing H5N1 whole inactivated influenza virus vaccine (WIV). The stability of the H5N1 hemagglutinin was assessed by measuring its hemagglutination activity. Overall, various compositions of trehalose and pullulan successfully stabilized β-galactosidase and WIV in ODFs. WIV incorporated in ODFs showed excellent stability even at challenging storage conditions (60 °C/0% relative humidity or 30 °C/56% relative humidity) for 4 weeks. Except for sugars, the polymeric component of ODFs, i.e., hypromellose, possibly improved stability of WIV as well. In conclusion, ODFs may be suitable for delivering of WIV to the oral cavity and can possibly serve as an alternative for injections.


Introduction
Influenza is one of the most serious infectious diseases responsible for high morbidity and mortality [1]. Vaccination is an efficient method to control yearly influenza epidemics and may help to combat occasional pandemics [2]. Except for Flumist ® , a live attenuated influenza vaccine that is administered intranasally, other marketed influenza vaccines are administered via the parenteral route. The disadvantage of injections, however, is that they need to be administered by trained health care personnel. In addition, needle stick injuries may occur by which infectious diseases can be transmitted. Finally, compliance is jeopardized for individuals with needle phobia.
Vaccination by the buccal or sublingual route is non-invasive, user-friendly and safe, and may be an attractive alternative delivery route. Buccal or sublingual vaccination is easy for self-administration [3][4][5][6]. Moreover, buccal or sublingual administration of influenza vaccine has been shown to induce mucosal immune responses in lungs, which plays an important role in neutralizing influenza virus directly at

Preparation of the Casting Solution and ODFs
The casting solution as developed by Visser et al. was used to prepare plain ODFs [23]. HPMC, carbomer 974P, disodium edetate, trometamol, and glycerol 85% were added to water under constant stirring at 1000 rpm. After complete dissolution, the entrapped air bubbles were removed from the solution by stirring at low speed (100 rpm) for an additional 48 h. The solution was cast onto a release liner (Primeliner 410/36, Loparex, Apeldoorn, The Netherlands) at the speed of 10 mm/s by using a coatmaster (Erichsen, Hemer, Germany) and with a casting height of 1000 µm. The ODFs were dried on the plate of the coatmaster at 30 • C for 10 min before pipetting the sugar solutions containing β-galactosidase or WIV onto them.

Preparation of Sugar Solutions with Antigen and Antigen Incorporated ODFs
Various solutions with either β-galactosidase or WIV incorporated were prepared in 2 mM HEPES buffer pH 7.4 as listed in Tables 1 and 2, respectively. In some formulations, BSA was incorporated to reduce potential absorption of antigen onto the wall of the glass container (beaker) during preparation or onto the pipetting tips. Methylene blue was used to make the antigen dots visible.
Antigen solutions were pipetted onto the ODFs by using a grid pattern at a volume of 6 µL. The ODFs were air-dried for 4 h at 30 • C (50-60% relative humidity) on the plate of the coatmaster or vacuum-dried for 24 h (using a Christ Epsilon 2-4 lyophilizer (Salm & Kipp, Breukelen, The Netherlands). The shelf temperature was maintained at 0 • C for 10 min at 0.05 mbar after which the chamber pressure was reduced to 0.03 mbar for 1 h. Subsequently, shelf temperature was increased at 0.05 • C/min to 20 • C while keeping the chamber pressure at 0.03 mbar [24].
The ODFs with β-galactosidase incorporated were punched in squares of 1.8 × 1.8 cm using an Artemio perforator (Artemio, Wavre, Belgium). Each of these ODFs had six β-galactosidase dots and the ODFs were sealed in a plastic bag and stored at −20 • C until characterization. Plain ODFs were compared with β-galactosidase containing ODFs for uniformity of weight and thickness, disintegration, and mechanical properties. ODFs containing two dots of β-galactosidase were put into Eppendorf tubes and stored at −20 • C and used for β-galactosidase enzymatic activity assay within 24 h after preparation. We assumed that β-galactosidase did not lose any activity during 24 h of storage at −20 • C.
ODFs with one dot of WIV containing 5 µg of protein were cut and also put into Eppendorf tubes. To investigate the effects of the excipients of the ODFs on the stability of WIV, WIV solutions were also dried as follows. WIV dots were prepared by pipetting 6 µL of WIV solution directly onto release liner, thus without ODFs, followed by air-or vacuum-drying as described above. The release liner was cut into pieces containing one WIV dot, which were put in Eppendorf tubes. Eppendorf tubes were stored at −20 • C and analyzed within 24 h after preparation by the hemagglutination assay as described below. We assumed that WIV did not lose any activity during 24 h of storage at −20 • C.

Uniformity of Mass and Thickness
Six randomly chosen β-galactosidase containing ODFs of each formulation as well as plain ODFs were weighed individually on an analytical balance. The average weight and weight variation were calculated.
The thickness of ODFs was measured with a micro screw meter (Mitutoyo, Neuss, Germany) at five different points of the ODFs: in the corners and in the middle. The thickness of each film was considered as the average of five points.

Disintegration Time
The disintegration time of ODFs with β-galactosidase incorporated and plain ODFs was determined with an adapted slide frame method as previously published [25]. The ODFs (n = 6) were clamped in an arm, which moved up and down at a frequency of 30 ± 1 cycles per min, over a distance of 55 ± 2 mm in a water bath at 37 • C ± 1 • C. The time at which ODFs were completely dissolved was recorded as the disintegration time. The endpoint was judged by visual inspection.

Mechanical Properties
The mechanical properties of ODFs with β-galactosidase incorporated and plain ODFs were analyzed using an Instron series 5500 load frames with a load cell of 100 N (Instron, Norwood, USA) [13,18,25]. ODF were cut into a bone shape according to ISO-527 standard (plastics-determination of tensile properties) (NEN-EN-ISO, 2012). The ODFs (n = 8) were fixed between two clamps positioned at a distance of 4 mm. Subsequently, the clamps were moved away from each other with a cross-head speed of 50 mm/min until tearing or breakage of the ODFs. Tensile strength (N/mm 2 ) and elongation at break (%) were recorded and automatically calculated by using Instron Merlin (series IX).

Process and Storage Stability Testing
The process and storage stability of β-galactosidase or WIV incorporated in ODFs was investigated. The storage stability was tested under closed vial conditions (0% relative humidity (RH)) at 4 • C, 30 • C and 60 • C, and under open vial conditions at 30 • C and 56% RH, generated by using a saturated sodium bromide solution [17,26]. The enzymatic activity of β-galactosidase and hemagglutination capacity of WIV were assessed immediately after the drying process (process stability) and after 1, 2 and 4 weeks of storage (storage stability). To investigate the storage stability of WIV without ODFs, the hemagglutination capacity of WIV dots was evaluated after 1, 2, 4 and 7 days of storage.

β-Galactosidase Enzymatic Activity Assay
The β-galactosidase activity was determined using a kinetic enzymatic assay, based on the conversion of a colorless substrate, o-nitrophenylgalactoside, into the yellow colored product, o-nitrophenol, by β-galactosidase [17,18]. Firstly, ODFs containing two dots of β-galactosidase were cut into small pieces and dissolved in 1.0 mL of 0.1 M phosphate buffer (pH 7.3), after which samples were diluted fivefold with enzyme diluent solution containing 0.1% BSA and 1 mM MgCl 2 in 50 mM phosphate buffer. Subsequently, 20 µL samples (n = 3) were pipetted into each well of a 96-well microplate (Greiner Bio-One, F shape), followed by 200 µL of 1.4 mM MgCl 2 in 0.1 M phosphate buffer. The plate was incubated at 37 • C for 10 min. Then, 20 µL of 50 mM substrate o-nitrophenyl-galactoside was added. The absorption was measured at 405 nm for 15 min at 37 • C with 30 s intervals (Synergy HT Microplate Reader, BioTek Instruments, Winooski, VT). The β-galactosidase activity was calculated from the slope of this conversion. All measurements were performed in triplicate.

Hemagglutination Assay
The activity of WIV in ODFs or in dots was assessed by the hemagglutination assay performed as previously described [22,27]. HA glycoprotein at the surface of WIV is able to bind to red blood cells Pharmaceutics 2020, 12, 245 6 of 14 (RBCs) and cause RBCs agglutination. In the absence of virus particles, RBCs precipitate to the bottom of the well by gravity, showing a red-colored dot in the well. In the presence of virus, RBCs clump together and no red dot is formed.
ODFs or WIV dots were dissolved in 0.1 M phosphate buffer (pH 7.3) to obtain a WIV concentration of 0.05 µg/µL. Subsequently, 100 µL of this solution was pipetted into 96-well V bottom plates and two-fold serially diluted. Thereafter, 50 µL of 1.5% guinea pig RBCs were added to each well and hemagglutination titers were read through visual inspection after 2 h incubation at room temperature. Hemagglutination titers were expressed as the log 2 of the highest dilution where RBCs agglutination occurred. All measurements were performed in triplicate.

Statistical Analysis
The results were statistically analyzed using one-way analysis of variance (ANOVA). A p-value < 0.05 was considered as significantly different. p-value less than 0.05, 0.01, 0.001, and 0.0001 are denoted by *, **, *** and ****, respectively. The graphs and curve fittings were performed using GraphPad Prism version 6.0 (GraphPad Prism Software, Inc., La Jolla, CA, USA).

Results and Discussion
β-Galactosidase was selected as a model antigen because it is readily available and relatively unstable [20]. The remaining enzymatic activity of β-galactosidase after incorporation in the ODFs and after subsequent storage was measured. In addition, uniformity of mass and thickness, disintegration and mechanical properties of the ODFs with β-galactosidase incorporated were determined. Subsequently, formulations with the best stability were applied for the incorporation of WIV into ODFs. The stability of WIV incorporated in ODFs and the influence of excipients of ODFs on WIV activity were investigated as well.  Tables 3 and 4, incorporation of β-galactosidase together with sugars in ODFs showed acceptable uniformity of mass and thickness with low standard deviations. All ODFs disintegrated within 30 s, which is recommended by U.S. Food and Drug Administration (FDA) for orally disintegrating tablets [28]. Furthermore, vacuum-dried ODFs showed slightly shorter (but not significantly different) disintegration time than air-dried ODFs. (b) Mechanical Properties As can be seen in Tables 3 and 4, by the incorporation of sugars, the ODFs had slightly lower tensile strength and elongation at break than plain ODFs (p < 0.0001), which means they became more brittle and thus less flexible. Furthermore, ODFs with the highest trehalose concentration (Trehalose (1.0) and BSA-Trehalose (1.0)) had the lowest tensile strength and elongation at break in both air-and vacuum-dried ODFs, which means they were the most brittle and fragile. Additionally, in a previous study, we found that incorporation of increasing amounts of trehalose in ODFs resulted in increasing deterioration of the mechanical properties [18]. This phenomenon can be explained by the low molecular weight of trehalose, which makes it a poor film former. Vacuum-dried ODFs showed a slightly lower (but not significantly different) tensile strength and elongation at break than air-dried ODFs.

ODFs with β-Galactosidase
Breaks sometimes occurred during handling or during the drying process for ODFs with highest trehalose concentration.

Enzymatic Activity of β-Galactosidase
Due to its ready availability and the straightforward quantitation by determination of its enzymatic activity, β-galactosidase has been widely used as model protein for stabilization by sugars [16,17]. β-Galactosidase formulated with trehalose by freeze-drying was found to be the most stable formulation when compared with other sugars (i.e., dextran 70 kDa, dextran 6 kDa and inulin) [16]. In a study by Lipiäinen et al. [29], melibiose or trehalose was used as stabilizing excipient for β-galactosidase by spray drying, and its activity remained for 30 days at 40 • C.
Immediately after drying, β-galactosidase activity of ODFs formulated with sugars was significantly higher than without sugar, independent of the drying method (Figure 1). The different sugar containing formulations had slight differences in process stability of β-galactosidase. Trehalose (0.5) showed the lowest process stability, 62% for air-drying ODFs and 65% for vacuum-drying ODFs. BSA-Trehalose (1.0) and Trehalose (1.0) exhibited the highest process stability, ending up at around 85% for both air-and vacuum-dried ODFs. Furthermore, the use of BSA in β-galactosidase incorporated sugar solutions seemed to have slightly positive effect on the process stability of β-galactosidase, however, as mentioned, the differences were minor.

ODFs with WIV Incorporated
Based on the results obtained with β-galactosidase, the formulations BSA-Trehalose (0.4) pullulan (0.1), Trehalose (0.4) pullulan (0.1) and BSA-Trehalose (1.0) were applied for incorporation of WIV into ODFs. A WIV solution without sugar was also pipetted onto ODFs as a negative control. WIV dots were prepared to investigate the influence of excipients of ODFs on the stability of WIV. Storage at 4 • C/0% RH for 4 weeks had no significant effect on β-galactosidase activity for both airand vacuum-dried ODFs when sugars were incorporated in the formulation ( Figure 1A,B). Although incorporation of β-galactosidase in ODFs without sugar did not result in a decline of enzymatic activity Pharmaceutics 2020, 12, 245 9 of 14 during the first 2 weeks of storage, activity decreased to 20% upon an additional 2 weeks of storage. Therefore, it can be concluded that when properly formulated, storage at 4 • C/0% RH seems a suitable storage condition for ODFs containing β-galactosidase.

Biological Activity of WIV Incorporated into ODFs
During storage at 4 • C/0% RH for 4 weeks, no distinction between the stabilizing capacities of the different sugar containing formulations could be made, because the ODFs were exposed to more challenging storage conditions, i.e., 30 • C/0% RH. During 4 weeks' storage at this condition, β-galactosidase incorporated in ODFs with both pullulan and trehalose with and without BSA in air-dried ODFs had better storage stability than other formulations, showing a remaining enzymatic activity of 40-50% ( Figure 1C,D). For example, air-dried ODFs with the formulations Trehalose (0.5), Trehalose (0.75), Trehalose (1.0) and BSA-Trehalose (1.0) exhibited remaining β-galactosidase activities below 20%, while air-dried ODFs with β-galactosidase incorporated without sugar almost completely lost its activity. Compared to air-dried ODFs, β-galactosidase incorporated in vacuum-dried ODFs generally showed a better storage stability, which might be explained by the fact that the vacuum-drying process (24 h) was longer than the air-drying process (4 h). During the vacuum-drying, the mobility of sugar reduced in a slower rate, which probably contributes to the better encapsulation of β-galactosidase by sugar. After 4 weeks' storage at 30 • C/0% RH, the remaining β-galactosidase activity was between 20-60% when sugars were present. The two formulations with BSA incorporated in vacuum-dried ODFs showed good storage stability as well, with a remaining β-galactosidase activity of 50-60% after 4 weeks. β-Galactosidase incorporated in vacuum-dried ODFs without sugar showed the lowest storage stability (12%).

ODFs with WIV Incorporated
Based on the results obtained with β-galactosidase, the formulations BSA-Trehalose (0.4) pullulan (0.1), Trehalose (0.4) pullulan (0.1) and BSA-Trehalose (1.0) were applied for incorporation of WIV into ODFs. A WIV solution without sugar was also pipetted onto ODFs as a negative control. WIV dots were prepared to investigate the influence of excipients of ODFs on the stability of WIV.

Biological Activity of WIV Incorporated into ODFs
The biological activity of HA of WIV was determined by the hemagglutination assay. In preliminary studies, we found that WIV incorporated in ODFs together with sugars was stable with a hemagglutination titer which remained constant at around 5 log 2 during 8 weeks' storage at 4 • C/0% RH and even at 30 • C/0% RH ( Figure S1). Therefore, it was decided to expose WIV containing ODFs to more challenging storage conditions, i.e., 60 • C/0%RH and 30 • C/56% RH.
As shown in Figure 2, immediately after preparation, the hemagglutination titer was significantly higher when WIV was formulated with sugars than without, for both air-and vacuum-dried ODFs (p < 0.05). Upon storage for 4 weeks at 60 • C/0% RH, hemagglutination titers were reduced in all samples, while no differences were found between titers upon air-or vacuum-drying. The hemagglutination titers of WIV formulated without sugar decreased from around 4.5 log 2 immediately after preparation to around 3.5 log 2 after 4 weeks of storage. No significant difference in storage stability at 60 • C/0% RH was found between the different formulations containing sugars; i.e., they all resulted in reduction of the hemagglutination titer from around 6 log 2 immediately after preparation of the ODFs to around 5 log 2 after 4 weeks. When stored under high moisture conditions, i.e., 30 • C/56% RH, the stability of WIV showed a similar trend as for 60 • C/0% RH. WIV formulated without sugar only showed a slight decrease of hemagglutination titer during storage: i.e., from around 4.5 log 2 immediately after preparation to around 4 log 2 after 4 weeks. All formulations with sugars showed similar stabilities upon storage. The addition of pullulan did not significantly improve WIV stability since WIV formulated with only trehalose or with blends of trehalose and pullulan did not show a significant difference in its hemagglutination titer. In other words, WIV can be stabilized either by only trehalose or by a blend of pullulan and trehalose in ODFs, and remains quite stable for at least 4 weeks at both 60 • C/0% RH and 30 • C/56% RH.
Besides, the differences of the stability of WIV among various sugar containing formulations were not as pronounced as for β-galactosidase incorporated in ODFs. Furthermore, WIV incorporated in ODFs appeared to be much more stable than β-galactosidase incorporated in ODFs. Apparently, WIV has a higher intrinsic stability. A high stability of WIV incorporated in sugar glasses was also shown by Geeraedts et al. [19] who found a reduction of the hemagglutination titer of WIV derived from H5N1 influenza virus (NIBRG-14) freeze-dried in the presence of trehalose or inulin from 11 log 2 to 8 log 2 during 3 months of storage at 40 • C. Murugappan et al. [30] found that the hemagglutination titer of WIV derived from A/Hir/H3N2 influenza virus spray freeze-dried in the presence of inulin, dextran or dextran/trehalose mixture remained constant at around 10 log 2 and did not change during 3 months storage at 40 • C.
i.e., 30 °C/56% RH, the stability of WIV showed a similar trend as for 60 °C/0% RH. WIV formulated without sugar only showed a slight decrease of hemagglutination titer during storage: i.e., from around 4.5 log2 immediately after preparation to around 4 log2 after 4 weeks. All formulations with sugars showed similar stabilities upon storage. The addition of pullulan did not significantly improve WIV stability since WIV formulated with only trehalose or with blends of trehalose and pullulan did not show a significant difference in its hemagglutination titer. In other words, WIV can be stabilized either by only trehalose or by a blend of pullulan and trehalose in ODFs, and remains quite stable for at least 4 weeks at both 60 °C/0% RH and 30 °C/56% RH.
Besides, the differences of the stability of WIV among various sugar containing formulations were not as pronounced as for β-galactosidase incorporated in ODFs. Furthermore, WIV incorporated in ODFs appeared to be much more stable than β-galactosidase incorporated in ODFs. Apparently, WIV has a higher intrinsic stability. A high stability of WIV incorporated in sugar glasses was also shown by Geeraedts et al. [19] who found a reduction of the hemagglutination titer of WIV derived from H5N1 influenza virus (NIBRG-14) freeze-dried in the presence of trehalose or inulin from 11 log2 to 8 log2 during 3 months of storage at 40 °C. Murugappan et al. [30] found that the hemagglutination titer of WIV derived from A/Hir/H3N2 influenza virus spray freeze-dried in the presence of inulin, dextran or dextran/trehalose mixture remained constant at around 10 log2 and did not change during 3 months storage at 40 °C.

Biological Activity of WIV without ODFs
In the previous part of this study, WIV was incorporated in ODFs by pipetting the sugar solution with antigen onto plain ODFs followed by air-or vacuum-drying. As the plain ODFs were composed

Biological Activity of WIV without ODFs
In the previous part of this study, WIV was incorporated in ODFs by pipetting the sugar solution with antigen onto plain ODFs followed by air-or vacuum-drying. As the plain ODFs were composed of readily water-soluble components (i.e., predominately HPMC), the pipetted solution will locally and partially dissolve these. As a consequence, after drying the antigen will not only be encapsulated by the sugars when present in the pipetting solution, but also by ODF components (HPMC), which may affect stability [31,32]. In order to investigate the stabilizing or destabilizing effects of ODF components, WIV dots that were prepared by pipetting sugar/WIV solution directly onto the release liner were analyzed. The samples were stored at 60 • C/0% RH or 30 • C/56% RH and after different time intervals, the hemagglutination titers of WIV were determined. Figure 3, the process stability of WIV dots (around 3 log 2 ) was significantly lower than that of WIV incorporated in ODFs. Upon storage, WIV without ODFs were more sensitive to temperature than to humidity. Both air-and vacuum-dried WIV dots without sugar fully lost their activity after 2 days of storage at 60 • C/0% RH. In contrast, air-dried WIV dots with sugar had a remaining hemagglutination titer of around 1 log 2 after 7 days of storage at 60 • C/0% RH. Vacuumdried WIV dots showed similar stability as air-dried WIV samples upon storage. At 30 • C/56% RH storage condition, the storage stability of WIV dots was slightly higher than that at 60 • C/0% RH. WIV dots formulated without sugar had lowest hemagglutination titer as well, ending up at 0.5 log 2 after 7 days of storage. The storage stability of WIV dots formulated with sugars decreased gradually and reached around 2 log 2 after 7 days.

As shown in
Compared with WIV incorporated in ODFs, WIV dots had significantly lower hemagglutination titers. The dramatic loss of WIV activity indicates the significant protective ability of ODFs on WIV. The major component of ODFs' HPMC probably contributes to improve stability of WIV. Furthermore, the slight dissolution of ODF on the spot of pipetting may cause the encapsulation of WIV, which encloses it not only by sugar but also by the components of ODFs, as a protection layer at the surface of WIV against higher temperatures and moisture. Consequently, not only trehalose and pullulan, but also ODF itself improves maintaining WIV activity, even at challenging storage conditions (60 • C/0% RH and 30 • C/56% RH).
of readily water-soluble components (i.e., predominately HPMC), the pipetted solution will locally and partially dissolve these. As a consequence, after drying the antigen will not only be encapsulated by the sugars when present in the pipetting solution, but also by ODF components (HPMC), which may affect stability [31,32]. In order to investigate the stabilizing or destabilizing effects of ODF components, WIV dots that were prepared by pipetting sugar/WIV solution directly onto the release liner were analyzed. The samples were stored at 60 °C /0% RH or 30 °C /56% RH and after different time intervals, the hemagglutination titers of WIV were determined.
As shown in Figure 3, the process stability of WIV dots (around 3 log2) was significantly lower than that of WIV incorporated in ODFs. Upon storage, WIV without ODFs were more sensitive to temperature than to humidity. Both air-and vacuum-dried WIV dots without sugar fully lost their activity after 2 days of storage at 60 °C /0% RH. In contrast, air-dried WIV dots with sugar had a remaining hemagglutination titer of around 1 log2 after 7 days of storage at 60 °C /0% RH. Vacuumdried WIV dots showed similar stability as air-dried WIV samples upon storage. At 30 °C /56% RH storage condition, the storage stability of WIV dots was slightly higher than that at 60 °C /0% RH. WIV dots formulated without sugar had lowest hemagglutination titer as well, ending up at 0.5 log2 after 7 days of storage. The storage stability of WIV dots formulated with sugars decreased gradually and reached around 2 log2 after 7 days.
Compared with WIV incorporated in ODFs, WIV dots had significantly lower hemagglutination titers. The dramatic loss of WIV activity indicates the significant protective ability of ODFs on WIV. The major component of ODFs' HPMC probably contributes to improve stability of WIV. Furthermore, the slight dissolution of ODF on the spot of pipetting may cause the encapsulation of WIV, which encloses it not only by sugar but also by the components of ODFs, as a protection layer at the surface of WIV against higher temperatures and moisture. Consequently, not only trehalose and pullulan, but also ODF itself improves maintaining WIV activity, even at challenging storage conditions (60 °C/0% RH and 30 °C/56% RH).  D). Hemagglutination titers are represented as log 2 titers with significance indicated as * p < 0.05. ** p < 0.01.*** p < 0.001 and **** p < 0.0001.

Conclusions
ODFs with antigens (β-galactosidase or WIV) incorporated could be successfully prepared by pipetting solutions containing antigen onto plain ODFs followed by air-or vacuum-drying.
The biological activity of WIV incorporated in ODFs was better preserved when formulated with sugars than without, indicating the protecting effect of sugars on WIV stability. Moreover, WIV incorporated in ODFs together with sugars showed excellent stability even when exposed to challenging storage conditions (60 • C/0% RH and 30 • C/56% RH) for 4 weeks. However, WIV air-or vacuum-dried without ODFs substantially lost its activity after 7 days of storage under the same conditions. Therefore, not only trehalose and pullulan, but also the components of the ODF (predominately HPMC) improved the stability of the WIV. Overall, this study took a step towards the development of a stable user-friendly dosage form to deliver WIV to the oral cavity.
In this research, WIV vaccine was pipetted onto ODFs. Obviously, this pipetting technique cannot be used for large-scale production. However, several industrially-applied printing techniques, e.g., 3D printing by 3D bioplotter ® , could replace the pipetting technique enabling large-scale production.