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Coordinated DNA Replication by the Bacteriophage T4 Replisome

Pennsylvania State University, Department of Chemistry, 414 Wartik Laboratory, University Park, PA 16802, USA
Author to whom correspondence should be addressed.
Academic Editor: David Boehr
Viruses 2015, 7(6), 3186-3200;
Received: 11 May 2015 / Revised: 12 June 2015 / Accepted: 16 June 2015 / Published: 19 June 2015
The T4 bacteriophage encodes eight proteins, which are sufficient to carry out coordinated leading and lagging strand DNA synthesis. These purified proteins have been used to reconstitute DNA synthesis in vitro and are a well-characterized model system. Recent work on the T4 replisome has yielded more detailed insight into the dynamics and coordination of proteins at the replication fork. Since the leading and lagging strands are synthesized in opposite directions, coordination of DNA synthesis as well as priming and unwinding is accomplished by several protein complexes. These protein complexes serve to link catalytic activities and physically tether proteins to the replication fork. Essential to both leading and lagging strand synthesis is the formation of a holoenzyme complex composed of the polymerase and a processivity clamp. The two holoenzymes form a dimer allowing the lagging strand polymerase to be retained within the replisome after completion of each Okazaki fragment. The helicase and primase also form a complex known as the primosome, which unwinds the duplex DNA while also synthesizing primers on the lagging strand. Future studies will likely focus on defining the orientations and architecture of protein complexes at the replication fork. View Full-Text
Keywords: DNA replication; T4 bacteriophage; replisome DNA replication; T4 bacteriophage; replisome
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Noble, E.; Spiering, M.M.; Benkovic, S.J. Coordinated DNA Replication by the Bacteriophage T4 Replisome. Viruses 2015, 7, 3186-3200.

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