Investigating the Potential Shared Molecular Mechanisms between COVID-19 and Alzheimer’s Disease via Transcriptomic Analysis

SARS-CoV-2 caused the COVID-19 pandemic. COVID-19 may elevate the risk of cognitive impairment and even cause dementia in infected individuals; it may accelerate cognitive decline in elderly patients with dementia, possibly in Alzheimer’s disease (AD) patients. However, the mechanisms underlying the interplay between AD and COVID-19 are still unclear. To investigate the underlying mechanisms and associations between AD progression and SARS-CoV-2 infection, we conducted a series of bioinformatics research into SARS-CoV-2-infected cells, COVID-19 patients, AD patients, and SARS-CoV-2-infected AD patients. We identified the common differentially expressed genes (DEGs) in COVID-19 patients, AD patients, and SARS-CoV-2-infected cells, and these DEGs are enriched in certain pathways, such as immune responses and cytokine storms. We constructed the gene interaction network with the signaling transduction module in the center and identified IRF7, STAT1, STAT2, and OAS1 as the hub genes. We also checked the correlations between several key transcription factors and the SARS-CoV-2 and COVID-19 pathway-related genes. We observed that ACE2 expression is positively correlated with IRF7 expression in AD and coronavirus infections, and interestingly, IRF7 is significantly upregulated in response to different RNA virus infections. Further snRNA-seq analysis indicates that NRGN neurons or endothelial cells may be responsible for the increase in ACE2 and IRF7 expression after SARS-CoV-2 infection. The positive correlation between ACE2 and IRF7 expressions is confirmed in the hippocampal formation (HF) of SARS-CoV-2-infected AD patients. Our findings could contribute to the investigation of the molecular mechanisms underlying the interplay between AD and COVID-19 and to the development of effective therapeutic strategies for AD patients with COVID-19.


Introduction
The global COVID-19 pandemic, caused by SARS-CoV-2 [1,2], has claimed millions of lives and imposed a heavy economic burden on individuals and countries worldwide.The SARS-CoV-2 virus primarily attacks the respiratory system, and COVID-19 patients commonly develop symptoms such as fever, cough, fatigue, and difficulty breathing [3].However, there is increasing evidence suggesting that COVID-19 patients may also suffer from certain neurological symptoms, including headaches, loss of smell and taste, stroke, and delirium [4,5].Even after recovering from COVID-19, individuals have reported experiencing cognitive and psychological symptoms, often referred to as "COVID-19 brain fog", which includes fatigue, poor concentration, and difficulties Viruses 2024, 16, 100 2 of 26 with memory and sleep [6].These neurological symptoms have attracted researchers to the topic of evaluating not only the short-term but also the long-term impact of COVID-19 on global public health, including different levels of impairments in the nervous system [7][8][9][10][11].
Older people, especially those with underlying medical comorbidities, such as heart disease, diabetes, cancer, and dementia, are much more susceptible to the severe illness of COVID-19 [12][13][14].Alzheimer's disease, the most common form of dementia, causes morbidity and mortality in aging populations worldwide [15].Some clinical studies have begun to investigate the interactions between AD and COVID-19.Clearly, AD patients are more susceptible to SARS-CoV-2 [14] and also have a higher mortality rate [16,17].Therefore, some researchers have paid close attention to the impact of COVID-19 on AD [18,19].Several studies with large cohorts have shown that COVID-19 may elevate the risk level of cognitive impairment and dementia in hospitalized [20,21] patients, and sequelae, such as mental health disorders and neurocognitive disorders in non-hospitalized [22] COVID-19 patients, and it is possible that SARS-CoV-2 infection may accelerate cognitive decline in AD patients [8,23,24].
However, the mechanisms underlying the interplay between AD and COVID-19 remain unclear, and investigating the molecular mechanisms is critical for developing effective therapeutic strategies [25].One possibility is the direct access of the SARS-CoV-2 virus into the brain, potentially via the olfactory tract [7,26] or through the infiltration of infected immune cells [7,27].Several genes have been identified as key factors for SARS-CoV-2 infection, including ACE2 [1], NRP1 [28], and BSG [29] as the viral spike (S) protein docking receptors, and TMPRSS2 [30], cathepsin B (CTSB) and FURIN [7,31] as the S protein priming protease to facilitate viral cell entry and replication.The olfactory tract has been shown to have a high level of ACE2 [23,32], and the expression levels of ACE2 are also elevated in AD patients [33].Supportive evidence for the infiltration of infected immune cells includes the observation of viral RNA in the macrophages of COVID-19 patients' bronchoalveolar lavage by single-cell RNA-seq data [27].Notably, there are no direct autopsy data or cerebrospinal fluid (CSF) studies [5,34] showing the presence of SARS-CoV-2 in the brain so far.
Another possibility concerning the impact of COVID-19 on AD is systemic inflammation and immune dysregulation, either in the central nervous system (CNS) via the overstimulation of the cytokine storm [35,36] or microvascular injury due to perfusion defects [34].The evidence for this possibility is as follows: (i) the cytokine storm has been observed in severe patients with COVID-19 [35]; (ii) circulating levels of IL-6, IL1b, and TNF were elevated in most COVID-19 patients [7,37]; (iii) Toll-like receptors (TLR), such as TLR2, mediated the inflammation induced by the SARS-CoV-2 spike protein through the NF-kB pathway [38]; (iv) the SARS-CoV-2 spike protein could alter the human blood-brain barrier (BBB) in vitro [39], and the S1 protein could cross the BBB in mice [40]; and (v) the infection of SARS-CoV-2 has increased the circulating concentrations of some nuclear proteins like high mobility group box 1 (HMGB1), while HMGB1 could enter the brain by breaking down the integrity of the BBB, and then induce the innate immune response [41].
To explore the molecular mechanisms of SARS-CoV-2 infection, intensive multi-omics datasets, including bulk RNA-seq, single-cell RNA-seq (scRNA-seq), and single-nucleus RNA-seq (snRNA-seq) data, have been generated for COVID-19 patients and SARS-CoV-2infected cells [9, 27,42].By integrating the large-scale gene expression data of AD [43], this research aims to investigate the underlying mechanisms and interactions between AD progression and SARS-CoV-2 infection (Tables 1 and S1).As the workflow in Figure 1 shows, we performed a series of bioinformatics analyses in SARS-CoV-2-infected cells, COVID-19 patients, and AD patients.We identified the common differentially expressed genes (DEGs) after SARS-CoV-2 infection in AD patients, and these DEGs are enriched in certain pathways like immune responses, cytokine storms, the Toll-like receptor signaling pathway, and NOD-like receptor signaling pathway.We further constructed the gene interaction network of seven major pathway modules with the signaling transduction module in the center, and IRF7, STAT1, STAT2, and OAS1 were identified as the hub genes.Interestingly, IRF7 is a shared DEG in AD patients and also functions as a transcription factor that may regulate the expression of ACE2.Importantly, we observed a significant up-regulation of IRF7 upon various RNA virus infections, and the expression of ACE2 was positively correlated with IRF7 expression in both AD and coronavirus infections.Our further analysis using snRNA-seq suggests that NRGN neurons or endothelial cells may be responsible for the increase in ACE2 and IRF7 expression after SARS-CoV-2 infection.We observed a positive correlation between ACE2 and IRF7 expressions in the hippocampal formation (HF) of SARS-CoV-2-infected AD patients.Our comprehensive analysis may provide supportive evidence of potential brain invasion, as well as strong evidence of neuroinflammation involving immune-related pathways after SARS-CoV-2 infection in AD patients.These findings contribute to the investigation of the molecular mechanisms underlying the interaction between AD and COVID-19 and the development of therapeutic strategies for AD patients with COVID-19.Then, classical enrichment analyses, including the GO and KEGG databases, were performed to assess the functional characteristics of the DEGs.Additionally, AD-related pathway enrichment scores in SARS-CoV-2 cells and patients were calculated.Furthermore, the hub genes were obtained using GeneMANIA analysis.Next, the Pearson correlation of hub genes, SARS-CoV-2 pathway-related genes, and SARS-CoV-2-related core transcription factors was performed in multiple datasets.
Finally, snRNA-Seq data (GSE159812) were included to determine which cell types exhibited significant enrichment for hub genes, SARS-CoV-2 pathway-related genes, and CoV2-related core transcription factors (TFs).

Data Sources
Gene expression data, including RNA-seq and microarray platforms, were downloaded from public databases, as summarized in Table 1.Briefly, the major datasets Alzheimer's disease (AD).First, the SARS-CoV-2 dataset GSE147507 and the AD dataset GSE95587 were used to find the common differentially expressed genes (DEGs between SARS-CoV-2 cells, COVID-19 patients and AD patients.DEGs were defined as |log2 fold change| ≥ 0.26 and p-value < 0.05.Then, classical enrichment analyses, including the GO and KEGG databases, were performed to assess the functional characteristics of the DEGs.Additionally, AD-related pathway enrichment scores in SARS-CoV-2 cells and patients were calculated.Furthermore, the hub genes were obtained using GeneMANIA analysis.Next, the Pearson correlation of hub genes, SARS-CoV-2 pathway-related genes, and SARS-CoV-2-related core transcription factors was performed in multiple datasets.Finally, snRNA-Seq data (GSE159812) were included to determine which cell types exhibited significant enrichment for hub genes, SARS-CoV-2 pathway-related genes, and CoV2-related core transcription factors (TFs).

Processing of Gene Expression Data
For RNA-seq data, if the raw fastq files were available, the raw reads were mapped to the human reference genome (hg38) using the STAR aligner v2.5.1b [65] with parameters, as suggested by the ENCODE guidelines [66] and the quantification of gene counts for each gene was obtained via the --quantMode parameter of STAR.The read count matrix of AD was obtained from GSE147507.Microarray data were normalized and log2-transformed using the limma R package (version 3.44.1).Differential expression analysis was conducted using the DESeq2 R package (version 1.28.1)[67] with a cutoff p-value < 0.01 and |log2(foldchange)| > 0.26 to identifyDEGs.All significant DEGs were visualized in a volcano plot generated using the R package ggplot2 (3.4.4) [68].Venn diagrams were created using the vennDiagram package (v1.7.3) in R [69].The gene counts were normalized using the DESeq2 estimateSizeFactorsForMatrix method.The normalized count data from DESeq2 was processed as log2(DESeq2NorCounts + 1) and then standardized using the min-max normalization method for visualization in a heatmap using the ComplexHeatmap package (v2.12.0) [70] in R.

snRNA-Seq Data Analysis
The gene expression matrix of snRNA-seq data was imputed in the Seurat R package (version 4.0.0)[71].PCA was used to cluster the cells with the top 10 principal components by running the FindNeighbors function with reduction = "pca" and dims = 1:10.The FindClusters function was used to identify clusters with a resolution = 0.3.The differential analysis of cell subgroups was performed using the Findmarker function provided by Seurat.Cell types were annotated for each cell type from a user-provided mtx file.

Gene Enrichment Analysis with Functional Annotation
To identify enriched GO terms for DEGs, we performed the pathway and process enrichment analyses for the selected gene list using Metascape (metascape.org,accessed on 13 November 2023) [72] with a p-value of less than 0.01 using the GO biological process module.Gene pathway analysis was further carried out using GeneAnalytics (geneanalytics.genecards.org,accessed on 21 November 2021) [73] via the GeneCards Suite.

Calculation of Pathway Enrichment Score (PES)
To determine the enrichment of AD-related pathways in CoV2 samples, we calculated a pathway enrichment score (PES) for each AD-related pathway.For each pathway gene set in 68 enriched pathways collected as previously described [64], we calculated the overlap of the pathway gene set with each CoV2 group: N = (AD pathway Genes ∩ each CoV2 DEGs).An example of AD-related PES in Calu3 was calculated using the following formula: The significance of PES was tested using the chi-squared test.PESs in lung COVID-19, NHBE, and Calu3 were calculated separately, filtering via the PES > 2 and p-value < 0.05.

Interaction Network of Up-Expressed DEGs upon SARS-CoV-2 Infection
The gene-gene interaction network was analyzed and visualized using the GeneMA-NIA v3.5.2 [74] plugin of Cytoscape v3.8.0 [75].The co-expression database was constructed with the expression of COV2 common up-expressed DEGs.The publicly available database of GeneMANIA was used for co-localization, genetic interactions, pathways, physical interactions, and predicted and shared protein domains.The gene interaction network was also verified using STRING v11.0 (https://string-db.org/,accessed on 13 March 2021) [76].

Transcription Factor Binding Sites (TFBS) Analysis with MEME
TFBS from the TRANSFAC database (v) (http://genexplain.com/transfac/,accessed on 7 June 2020) [77] was used to construct position weight matrices for the motif prediction.The promoter for each gene was defined as 1.5 kb upstream of TSS and 500 bp downstream of TSS.Local motif identification was performed via MAST (Motif Alignment and Search Tool) programs (http://www.sdsc.edu/MEME/meme/website/html,accessed on 13 January 2023) from the MEME software suite with default parameters [78].

Statistical Analysis
All statistical analyses were performed with the R statistical software using the Wilcoxon rank-sum test or the Chi-squared test.

Identification of DEGs Shared between AD and COVID-19 Patients
In our investigation into the potential increased susceptibility of AD patients to COVID-19 infection, we analyzed differential gene expression in both conditions.In the brain tissue of AD patients [43], compared to normal brains, we identified 3928 genes that were upregulated and 3328 genes that were downregulated (Figure 2A).Similarly, in the lung tissue of COVID-19 patients [42], we found 812 genes that were upregulated and 1891 genes that were downregulated (Figure 2B).Notably, there was a significant overlap in gene expression changes between the two conditions: 197 genes were commonly upregulated in both AD and COVID-19 patients, while 136 genes were commonly downregulated (Figure 2C).These shared gene expression patterns could indicate a biological link between AD and increased vulnerability to COVID-19 infection.
Subsequently, to identify the intersecting genes in AD and COVID-19 patients, we conducted a comprehensive GO BP and KEGG pathway enrichment analysis (Figures 2D and S1A).For the genes that were upregulated in both AD and COVID-19, the enrichment analysis revealed a significant association with pathways related to immune response and inflammation.Specifically, these genes were predominantly enriched in pathways such as myeloid leukocyte activation, cytokine-mediated signaling, the immune response-regulating signaling pathway, the defense response to other organisms, and cell chemotaxis.This suggests a possible shared mechanism involving heightened immune and inflammatory responses in both AD and COVID-19.Conversely, the downregulated intersecting genes were mainly enriched in pathways related to mitochondrial function, ion transport, neural development, and behavior (Figure 2D).The manipulation of mitochondria via SARS-CoV-2 could induce mitochondria dysfunction and then increase mitochondria-derived double-membrane vehicles in which the virus can hide and replicate [79].Mitochondria have been reported to be involved in the process of inflammation in both innate and adaptive immunity [80].When we combined the shared upregulated and downregulatedDEGs, the most enriched GO term emerged as myeloid leukocyte activation, alongside significant enrichment in the homeostasis of the number of cells pathway.Moreover, cell death is well known to play an important role in AD progression [64].This indicates the potential common pathway of neural and mitochondrial dysfunction in both conditions.These findings are consistent with our proposal that the altered enriched pathways involved in immune responses, cell death, and mitochondrial dysfunction upon SARS-CoV-2 infection are altered in similar patterns in AD, which may accelerate cognitive decline in AD patients.
These findings are consistent with our proposal that the altered enriched pathw volved in immune responses, cell death, and mitochondrial dysfunction upon SAR 2 infection are altered in similar patterns in AD, which may accelerate cognitive de AD patients.

Identification of DEGs Shared between SARS-CoV-2-Infected Calu-3 Cells and NHBE Cells
In our comprehensive study to understand the biological pathways implicated in the SARS-CoV-2 infection, we further conducted the analysis of gene expression changes in Calu-3 cells, human epithelial cells located on the pulmonary surface, and normal human bronchial epithelial (NHBE) cells following SARS-CoV-2 infection.Our findings revealed significant alterations in the gene expression in response to the virus in both cell types.In the Calu-3 cell line, a substantial number of genes were affected, with 3696 genes showing upregulation and 3738 genes that were downregulated (Figure 3A).In NHBE cells, the impact was also notable, albeit on a smaller scale, with 760 genes upregulated and 659 genes downregulated after infection (Figure 3B).We discovered that there were 364 genes that were consistently upregulated and 122 genes that were consistently downregulated in both Calu-3 and NHBE cells (Figure 3C).The commonly upregulated genes were primarily enriched in pathways that play pivotal roles in viral response mechanisms (Figures 3D and S1B).These include the response to the virus, the cytokine-mediated signaling pathway, the regulation of viral processes, and the response to type I interferon.Conserved signaling pathways observed across different cell lines infected with SARS-CoV-2, specifically cytokine-mediated signaling pathways and immune responses, may trigger systemic inflammation and a cytokine storm, resulting in a significant increase in pro-inflammatory cytokines.There is evidence suggesting that neuroinflammation can impact cognitive functions and accelerate the progression of neurodegenerative diseases [81].This implies that the inflammatory response induced by SARS-CoV-2 could potentially exacerbate the progression of AD.Conversely, the downregulated genes in both cell types were significantly enriched in pathways associated with cell cycle control and metabolic processes (Figure 3D).Key among these were the regulation of the mitotic cell cycle phase transition, the regulation of the mitotic cell cycle, the regulation of cyclin-dependent protein serine/threonine kinase activity, and the unsaturated fatty acid metabolic process.SARS-CoV-2's effect on cellular functions, such as the cell cycle and metabolic processes, as observed in SARS-CoV-2infected lung cells, might also occur in neural cells [82,83].Since AD already involves disruptions in neural cell functions, additional stress from SARS-CoV-2 could exacerbate these disruptions.

Changes in the Expression of Top AD DEGs in SARS-COV-2-Infected Cells and in COV 19 Patients
To investigate how SARS-CoV-2 infection affects AD progression, we also stu how the expression profiles of the top AD DEGs change after SARS-CoV-2 infection first identified the top 50 up-and 50 down-expressed DEGs in AD patients.We the vestigated the expression profiles of these top up-and down-regulated AD DEG COVID-19 patients, and also in NHBE and Calu-3 cells, before and after SARS-CoVfection (Figure 4A,B).This analysis was extended to include an examination of the to upregulated and top 50 downregulated genes across various datasets.Remarkably found that the expression patterns of these top differentially expressed genes in othe tasets were similar to the patterns observed in the GSE95587 dataset.This consist across multiple datasets underscores the potential significance of these genes in AD  To investigate how SARS-CoV-2 infection affects AD progression, we also studied how the expression profiles of the top AD DEGs change after SARS-CoV-2 infection.We first identified the top 50 up-and 50 down-expressed DEGs in AD patients.We then investigated the expression profiles of these top up-and down-regulated AD DEGs in COVID-19 patients, and also in NHBE and Calu-3 cells, before and after SARS-CoV-2 infection (Figure 4A,B).This analysis was extended to include an examination of the top 50 upregulated and top 50 downregulated genes across various datasets.Remarkably, we found that the expression patterns of these top differentially expressed genes in other datasets were similar to the patterns observed in the GSE95587 dataset.This consistency across multiple datasets underscores the potential significance of these genes in AD pathology (Figure 4C).We also performed GO and KEGG analysis on these top up-and down-expressed DEGs.The 50 up-expressed DEGs, are enriched in segmentation, histone modification, the negative regulation of the cell cycle, muscle organ development, and the response to a mechanical stimulus (Figures 4D and S1C), indicating the possible role of cell division in AD progression.The 50 down-expressed DEGs are enriched in the regulation of intracellular pH, the regulation of the G protein-coupled receptor signaling pathway, and plasma membrane-bounded cell projection assembly, highlighting the role of hypoxia in AD pathogenesis.Combining the up-and down-expressed DEGs, we found that the positive regulation of cold-induced thermogenesis and cellular response to abiotic stimulus are also enriched.Taken together, these enriched GO terms are consistent with the current knowledge that inflammatory responses and apoptosis play important roles in AD progression and also support the hypothesis that hypoxia could lead to chronic activation and the recruitment of pro-inflammatory immune cells [84].Among the top 50 up-regulated AD DEGs, 12 showed a similar up-expression pattern upon SARS-CoV-2 infection (Figure 4A), with more than 10% up-expressed in at least 2 of the 3 conditions (COVID-19 patients or 2 cell lines).NFKB1 shows almost the same expression pattern in AD patients, COVID-19 patients, and in NHBE and Calu-3 cells, highlighting the important role in both AD and COVID-19 progression.These 12 genes are enriched in GO in terms of chordate embryonic development (Figure 4D).Similarly, among the top 50 down-regulated AD DEGs, 54% of them (27 genes) are downexpressed upon SARS-CoV-2 infection.NDUFA (NDUFA9, NDUFAB1), ATF (ATP5F1A, ATP5PB, ATP5MC3, ATP6V1D, ATP6V1E1), PSMA (PSMA1, PSMA5), and MRPS (MRPS16, MRPS23) families are enriched in these 27 genes.The most enriched GO term for these 27 genes is the mitochondrial respiratory chain complex assembly, which highlights the critical role of mitochondria in AD and COVID-19 progression [79].When the common 12 up-expressed and 27 down-expressed DEGs are combined, these genes are enriched in a new GO term of antigen receptor-mediated signaling pathway, which again supports the observation that inflammatory responses play an essential role in both AD progression and SARS-CoV-2 infection.

Changes in the Expression of the Most Common DEGs Associated with SARS-CoV-2 Infection in AD Patients
We then asked what the expression levels of SARS-CoV-2 DEGs are in AD patients.We first compared the DEGs in SARS-CoV-2-infected Calu-3 and NHBE cells as well as in COVID-19 patients to identify the common DEGs upon SARS-CoV-2 infection (Figure 5A).We found 69 common DEGs that were up-expressed (left) and 7 common DEGs that were down-expressed (right) after SARS-CoV-2 infection.Subsequently, we checked the expression levels of these common DEGs in AD patients (Figure 5B,C).Among the 69 up-expressed genes, 39 genes were also identified to be up-expressed (fold change, FC > 1.1) in AD patients (Figure 5B), and 18 of these genes were also AD DEGs considering more stringent criteria (gene symbols in red, FC > 1.2, and p-value < 0.01).We also performed GO analysis on the common DEGs upon SARS-CoV-2 infection and found enrichment in the regulation of cytokine production and the response to interferon-alpha (Figures 5D and S1D), highlighting the important roles of interferons in SARS-CoV-2 infection, as supported by a recent study [85].Further GO analysis on the 39 genes sharing a similar up-regulated pattern revealed enrichment in the biological process involved in symbiotic interaction, the positive regulation of the defense response to the virus by the host, the receptor signaling pathway via JAK-STAT, adaptive immune response, and regulation of cell growth (Figure 5D).Among the 7 down-regulated genes, PALMD was also identified to be down-expressed in AD patients (FC < 0.9, Figure 5C).

IRF7 Plays Key Roles in Pathways Involved in Signaling Transduction in Both AD and SARS-CoV-2 Infection
We then identified the most common DEGs in AD patients, COVID-19 patients, and SARS-CoV-2-infected Calu-3 and NHBE cells (Figure 6A).In total, 18 genes were identified as common DEGs across AD patients, COVID-19 patients, and CoV2-infected Calu-3 and NHBE cells.These 18 essential genes include IRF7, IFIH1, IFITM2, etc., and GO and KEGG analysis indicate that these DEGs are enriched in the viral process, response to the virus, the regulation of response to cytokine stimulus, and leukocyte homeostasis, which again highlights the important roles of immune responses in both AD progression and SARS-CoV-2 infection (Figures 6B and S1E).

IRF7 Plays Key Roles in Pathways Involved in Signaling Transduction in Both AD SARS-CoV-2 Infection
We then identified the most common DEGs in AD patients, COVID-19 pa SARS-CoV-2-infected Calu-3 and NHBE cells (Figure 6A).In total, 18 genes w fied as common DEGs across AD patients, COVID-19 patients, and CoV2-infec and NHBE cells.These 18 essential genes include IRF7, IFIH1, IFITM2, etc., an KEGG analysis indicate that these DEGs are enriched in the viral process, respo virus, the regulation of response to cytokine stimulus, and leukocyte homeosta again highlights the important roles of immune responses in both AD progre SARS-CoV-2 infection (Figures 6B and S1E).In order to identify the essential hub genes in the interaction network, we classified all 69 up-expressed common DEGs upon SARS-CoV-2 infection into different pathways on the basis of their annotated functions (Figures 6C,D and S2).We found that some genes could be classified into several different pathways, such as IRF7, OAS1, STAT1, and STAT2, indicating their multifaceted roles (Figure 6D).According to the gene's physical interactions, colocalization, and co-expression, the gene interaction network with pathway annotation was constructed (Figure 6C).We found 7 major pathway modules, as shown in Figure 6C, with the signaling transduction module located in the center of the network.IRF7, STAT1, STAT2, and OAS1 are involved in all 9 enriched pathways (Figure 6D), indicating their important roles in regulating the gene expression network.Based on these observations, IRF7, STAT1, STAT2, and OAS1 have been identified as the essential genes, and the signaling transduction module is the core of the network.Among the four genes, IRF7 is the most up-regulated gene, while STAT1 is down-regulated in AD, suggesting that IRF7 plays a key role in the interaction network of regulating gene expression in both AD progression and SARS-CoV-2 infection.In the network, IRF7 may regulate immune responses through the following several nodes: LCN2 in the cytokine signaling module, LGAS9 and DAPP1 in the NF-kappaB signaling module, IFITM2, TRIM21 in the IFN signaling module, CD55 and S100P in the innate immune system module, IRAK3 and IFIH1 in the Toll-like receptor signaling module, or NAMPT and OAS3 in the NOD-like receptor signaling module.

IRF7 Is Significantly Up-Regulated upon Different RNA Virus Infections, and the Expression of ACE2 Is Positively Correlated with IRF7 Expression in Both AD and Coronavirus Infections
We further investigated the genes involved in the SARS-CoV-2 and COVID-19 pathway (WP4846) retrieved from the WikiPathways database [86].We first analyzed the expression levels of these genes in both AD patients, COVID-19 patients, and SARS-CoV-2-infected Calu-3 and NHBE cells (Figure 7A).We found that ACE2 and TLR7 are expressed at low levels in healthy brains, slightly increased in AD patients, while TMPRSS2/4 and SLC6A19 are extremely low-expressed, with nearly no expression, in AD patients.The expression levels of ACAT1 and CTSL show opposite trends in AD patients and COVID-19 patients.
To identify transcription factors (TFs) that may bind to the SARS-CoV-2 and COVID-19 pathway-related genes, we analyzed the upstream regulatory regions of these genes to predict transcription factor-binding sites (Figure 7B).We found 6 transcription factors among the 69 up-regulated genes in COVID-19 patients and SARS-CoV-2-infected cells (Figure 5B) that may bind to these genes, including genes in the STAT family (STAT1/2), GBP family (GBP1/5), IRF7 and PARP9 This highlights the essential regulatory roles of genes in the STAT and IRF family in SARS-CoV-2 infection, as evidenced by the hub genes of STAT2 and IRF7 in the network shown in Figure 6C.We then investigated the correlation between the expression of the SARS-CoV-2 and COVID-19 pathway-related genes and 4 major TFs that are up-regulated in AD (Figures 7C and S3).The ACE2 expression was positively correlated with the expression of IRF7 in Calu-3, NHBE, and AD patients, and the expression of TMPRSS2 was positively correlated with the expression of IRF7 in Calu-3 and AD patients (Figure 7C).Considering IRF7's role as both a DEG in AD and SARS-CoV-2 infection and also as a transcription factor that might bind with the human ACE2 promoter, which is very important for viral penetration into cells [87], we suspected that IRF7 might be a key factor for SARS-CoV-2 infection and AD progression.To study the function of IRF7 in AD progression and SARS-CoV-2 infection, we first checked the expression changes in IRF7 in other independent AD and SARS-CoV-2 datasets (Table 1).As expected, IRF7 expression is significantly increased in AD patients, SARS-CoV-2 infected cells, and also in patients infected with SARS-CoV-2 (Figure S4).To identify transcription factors (TFs) that may bind to the SARS-CoV-2 and COVID-19 pathway-related genes, we analyzed the upstream regulatory regions of these genes to predict transcription factor-binding sites (Figure 7B).We found 6 transcription factors among the 69 up-regulated genes in COVID-19 patients and SARS-CoV-2-infected cells (Figure 5B) that may bind to these genes, including genes in the STAT family (STAT1/2), We then extended the analysis to other RNA viruses, and the results indicated that the expression of IRF7 is also significantly increased in other RNA virus infections (Figure 7D), including SARS (p = 0.02), MERS (p = 0.0039) and HIV (p = 0.0032).Next, we checked the correlation between expressions of the SARS-CoV-2 and COVID-19 pathway-related genes and 4 major TFs in these datasets (Figure 7E) and observed that ACE2 expression is positively correlated with IRF7 expression in both SARS and MERS.The correlation between ACE2 and IRF7 expressions was not observed in white cells because ACE2 is not expressed in HIV infection (Figure S5).It can be noted that the positive correlation of ACE2 and IRF7 expression is not observed in some other independent datasets (Figure S5).Furthermore, we investigated the expression changes in the SARS-CoV-2 and COVID-19 pathway-related genes and 4 major TFs in different cell types in the human brain upon SARS-CoV-2 infection.The snRNA-seq analysis indicates that NRGN neurons or endothelial cells may be responsible for the elevated expression of ACE2 and IRF7 after SARS-CoV-2 infection (Figure 7F).Lastly, we observed the positive correlation between ACE2 and IRF7 expressions both in the frontal cortex of COVID-19 patients (Figure S5) and in the hippocampal formation (HF) of SARS-CoV-2-infected AD patients (Figure 7G).Taken together, these results support our hypothesis that IRF7 may regulate ACE2 expression and could be a critical factor for SARS-CoV-2 infection; IRF7 may modulate the immune responses in AD progression and other RNA virus infections.
We also checked the most common enriched pathways shared between COVID-19 patients and SARS-CoV-2-infected cells, with a cut-off of PES > 2 and p-value < 0.05 (Figure 8E).The shared enriched pathways include hematopoietic cell lineage, the NODlike receptor signaling pathway, Toll-like receptor signaling pathway, signal transduction through IL-1R, TNF signaling pathway, cytokine-cytokine receptor interaction, apoptosis, and osteoclast differentiation (Figure 8E).These commonly enriched pathways highlight the essential role of immune cell response and cytokines (such as IL-1), apoptosis, and the possible role of mitochondria (NOD-like receptor pathway) in both AD progression and SARS-CoV-2 infection.We also checked the most common enriched pathways shared between C patients and SARS-CoV-2-infected cells, with a cut-off of PES > 2 and p-value < 0.0 8E).The shared enriched pathways include hematopoietic cell lineage, the NO ceptor signaling pathway, Toll-like receptor signaling pathway, signal tran through IL-1R, TNF signaling pathway, cytokine-cytokine receptor interaction, a and osteoclast differentiation (Figure 8E).These commonly enriched pathways

Discussion
Recent research reported that COVID-19 may elevate the risk of cognitive impairment or even cause dementia in COVID-19 patients [18,19,21,88,89] and may possibly accelerate cognitive decline in AD patients [8,23].Here, we investigated the potential molecular mechanisms underlying the interplay between COVID-19 and AD.Using comprehensive bioinformatics methods to analyze massive multi-omics datasets for COVID-19 patients, SARS-CoV-2-infected cell lines, and the gene expression data of AD patients, we identified the common differentially expressed genes (DEGs) after SARS-CoV-2 infection and in AD patients, and these DEGs are enriched in certain pathways such as immune responses and cytokine storms.We also constructed the gene interaction network with the signaling transduction module in the center, and IRF7, STAT1, STAT2, and OAS1 were identified as the hub genes.We also checked the correlations between several major transcription factors and genes involved in the entry of SARS-CoV-2 into host cells.We observed that ACE2 expression is positively correlated with IRF7 expression in AD and coronavirus infections, and interestingly, IRF7 is significantly up-regulated in response to different RNA virus infections.These findings could help us to examine the different possible mechanisms of the effect COVID-19 has on AD.

IRF7 and SARS-CoV-2 Entry into the Brain
The functions of the brain may be impaired if SARS-CoV-2 can directly invade the brain, and the most convincing model for the neuroinvasion of SARS-CoV-2 is through the olfactory route.In the hypothesis, the virus gains access to the CNS via the neural-mucosal interface in the olfactory mucosa and eventually reaches the olfactory bulb [7].This supportive evidence includes the existence of SARS-CoV-2 RNA and the protein in human olfactory mucosa and the brain [26], human brain organoids, and mice brain [90]; independent autopsy studies also confirmed these observations [91], even in a 14-month-old child [92].However, some pieces of evidence against the hypothesis are also present, and it is controversial whether SARS-CoV-2 can enter the brain directly or not.Some studies reported that SARS-CoV-2 was not detectable in the brain [34] or CSF [5].Another evidence against the direct neuroinvasion hypothesis is that the expressions of both ACE2 and TMPRSS2 in the brains and neurons are very low, almost undetectable [7].However, this evidence cannot rule out the possibility of entry through other key factors, such as NRP1, BSG, CTSB, and FURIN, and the expression levels of BSG, NRP1, and FURIN are relatively high in the prefrontal cortex regions [7].Furthermore, even if the expression level of ACE2 is relatively low in normal neurons, it could be up-regulated by other transcription factors in some conditions, such as in AD patients, which may help the neuroinvasion of SARS-CoV-2.In our research, we found that the expression of ACE2 is up-regulated and positively correlated with the expression of IRF7 in AD.Further analysis indicated that IRF7 is significantly up-regulated upon different RNA virus infections, and IRF7 may bind directly to the ACE2 promoter to regulate the expression of ACE2 in humans.IRF7, which belongs to the interferon regulatory factor family, regulates many IFN-α genes, and IFN-α has been proven to regulate the expression of ACE2 [93].Based on these observations, we proposed that IRF7 can up-regulate the expression of ACE2, either directly (binding to the promoter) or indirectly (through IFN-α) in AD, which may help the brain's entry of SARS-CoV-2.Our model raised another way to provide supportive evidence of a direct brain invasion.

IRF7 as a Major Player in Signaling Transduction
Interferon regulatory factors (IRFs) are transcription factors that regulate the transcription of interferons (IFNs).This family contains nine members (IRF1-9) in mammals, and IRF7 is one of them [87].The major function of IRFs is signaling transduction in innate immune responses [87,[94][95][96].During viral infection, IRF pathways, mainly IRF3 and IRF7, are activated through signaling pathways triggered by different pattern-recognition receptors (PRRs), for example, transmembrane Toll-like receptors (TLRs), which can recognize the viral pathogen-associated molecular patterns (PAMPs) derived from invading pathogens [87,94,96].IRF7 has been reported to interact with IRF3 and is the closest to IRF3 in the IRF family [96,97].IRF3 has strict DNA-binding specificity (GAAANNGAAANN), while IRF7 has broader DNA-binding specificity (GAAWNYGAAANY).We investigated the potential TFBSs in the promoter region of ACE2 and predicted several IRF3/IRF7-binding sites in both human and mouse ACE2.IRF3 is constitutively expressed in many cells, while the expression of IRF7 is relatively low in most cell types and is only highly expressed in plasmacytoid dendritic cells (pDC), B cells, and monocytes [87].Interestingly, at the later stages of viral infection, the expression of IRF7 is significantly elevated via a positive feedback loop, in which type I IFNs induce IRF7 and IRF7 then induces expressions of several IFNα subtypes, such as IFNα2, α5, α6, and α8 [98,99].By binding to the IFN-α receptor (IFNAR), type I IFNs can activate the JAK-STAT signaling pathway, which is involved in immune response and cell death [94,99].It has been reported that SARS-CoV-2 infection could elevate the expression levels of IL-2/4/6/10, STAT1/2/3, and TNF-a [7,37].In this study, we constructed a gene interaction network with the signaling transduction module in the center, consisting of genes IRF7, STAT1, STAT2, and OAS1.We found that IRF7 is significantly up-regulated in both AD and COVID-19 and, together with STAT genes (such as STAT2), are located in the core of the signaling transduction module.As a major player in the signaling transduction pathway, IRF7 has been involved in the Toll-like receptor signaling pathway (the recognition of viral PAMPs), the IFN signaling pathway (IRF7 positive feedback looping), and the innate immune system in both SARS-CoV-2 infection and AD.

Roles of IRF7 in Different Virus Infections and Other Diseases
IRF7 was identified in 1997 in a latent Epstein-Barr virus (EBV) infection and was associated with the EBV latency program [100].A homozygous deficiency of IRF7 was reported to cause severe influenza and acute respiratory distress syndrome in a 2.5-year-old girl, which highlights the role of IRF7 in severe influenza [101].Few studies have focused on the roles of IRF7 in coronavirus infections [102], and our analysis indicated that IRF7 is significantly up-regulated upon different RNA virus infections, including SARS-CoV-2, SARS, MERS, and HIV.IRF7 was recently found to be a mediator of protection and was correlated with the risk of HIV-1 acquisition [103].We have also shown that IRF7 expression is remarkably elevated in AD patients.A recent study surveyed several transcriptomic datasets from AD patients and observed that IRF7 is up-regulated in some regions of the brain while it is also down-regulated in other regions in some samples [95], which implies that IRF7 has cell-specific regulatory roles.Our analysis based on snRNA-seq data indicated that IRF7 was up-regulated in NRGN neurons and macrophage cells upon SARS-CoV-2 infection.Besides the function of regulating immune responses through inducing type I IFN production, IRF7 also plays critical roles in the regulation of oncogenesis, apoptosis, and cell differentiation [87,104,105].As a common feature of massive neuronal death from apoptosis, apoptotic neurons and glial cells have been observed in AD [106,107].Our analysis indicated that the common DEGs of COVID-19 and AD are enriched in the pathway of apoptosis, suggesting that apoptosis might be involved in COVID-19 and AD pathophysiology, and IRF7 might play a critical role in apoptosis.

Epigenetic Regulation of IRF7 in SARS-CoV-2 Infection and AD Patients
As stated above, IRF7 is relatively low in most types of cells, while it is highly expressed in plasmacytoid dendritic cells (pDC), B cells, and monocytes, which indicates that IRF7 expression is cell-type specific.The cell-specific expression of IRF7 may be regulated through epigenetic mechanisms, such as chromosomal accessibility [108] and DNA methylation [109].The expression of IRF7 could be silenced through the hypermethylation of the CpG islands in the promoter region of IRF7 in human fibroblasts and 2fTGH [109].In a recent epigenome-wide association study (EWAS), the authors detected the methylation statuses of CpG sites in IRF7 via the methylation EPIC array in the peripheral blood samples of COVID-19 patients and found the different methylation statuses of CpG sites to be significantly associated with different disease severities [110].In our study, the expression levels of IRF7 are diverse in different cells and are upregulated upon different RNA virus infections, including SARS-CoV-2.It remains unclear whether the expression changes in IRF7 in these cells are regulated by DNA methylation.In another independent study focusing on the methylomes of peripheral blood samples from COVID-19 patients after a 3-month recovery [111], we found that the DNA methylation status of IRF7 has not been completely restored.These results indicate that epigenetic modifications may be Viruses 2024, 16, 100 20 of 26 involved in the regulation of IRF7 in SARS-CoV-2 infection and AD and the detection of the methylation statuses of some factors in the blood, such as IRF7, which could provide insights for diagnosis and prognosis.

Immune Dysregulation and Neuroinflammation in AD Patients with COVID-19
A critical feature of the maladaptive immune response in COVID-19 is a hyperinflammatory response followed by immunosuppression [35][36][37].In severe COVID-19 patients, the hyperinflammatory response, also called the cytokine storm, is a hallmark feature with elevated circulating levels of IL-2/4/6/10, STAT1/2/3, and TNF-a [7,37].This systemic inflammation may further aggravate neuroinflammation and increase the susceptibility of patients to other neurological syndromes in AD.The systemic inflammatory mediators may access the CNS and cause damage through an impaired BBB function, which was reported in neurodegenerative diseases and viral infections, including SARS-CoV-2 infection [39,40].Furthermore, microvascular injuries in the brain due to perfusion defects in SARS-CoV-2 infections were also reported [34].Using intensive pathway analyses, we identified the enrichment of the JAK-STAT cascade, including cytokine signaling and NF-kB and IFN signaling pathways in both AD and COVID-19 patients.These findings may partly explain the cognitive decline in AD patients with COVID-19.As we reported, the expression levels of some key genes (including IRF7, STAT2, OAS1, LCN2, LGAS9, DAPP1, IFITM2, TRIM21, CD55, S100P, IRAK3, and IFIH1) were significantly up-expressed in both AD and COVID-19 patients, with some of them also being evidenced by several other studies [13,18,110], which highlights the critical roles of immune response factors in neuroinflammation in AD patients with COVID-19.

Mitochondrial Dysfunction in Neuropathogenesis of SARS-CoV-2 Infections
Mitochondrial dysfunction and the disturbance of energy production are proposed to trigger neurodegenerative diseases [112].AD has been shown to be closely associated with various forms of mitochondrial dysfunctions, including low ATP production, excessive ROS production, calcium dyshomeostasis, and mitophagy [113].Mitochondria-generated ROS and oxidized mtDNA could be used as endogenous DAMPs and trigger NLRP3 (nucleotide-binding oligomerization domain (NOD)-like receptor protein 3) inflammasome complex formation [80].NLRP3 inflammasome activation could then contribute to the pathogenesis of AD by inducing and sustaining neuroinflammation [114].Mitochondrial dysfunction induced by manipulations of host mitochondria using SRAS-CoV-2 viral open reading frames (ORFs) has also been reported [115].A recent study reported that mitochondrial dysfunction, as the earliest feature of neurodegeneration, is a prelude to SARS-CoV-2-induced neuropathogenesis [79].In our study, the common down-regulated DEGs shared in AD and COVID-19 patients are enriched in mitochondrion organization and the mitochondrial respiratory chain complex assembly.Furthermore, we found that the NOD-like receptor signaling pathway is a highly enriched pathway shared in AD patients, COVID-19 patients, and SARS-CoV-2-infected Calu-3 and NHBE cells.All of these findings are consistent with current research focusing on mitochondrial functions in AD and COVID-19, indicating that mitochondrial dysfunction may have very important functions in the neuropathogenesis of SARS-CoV-2.
All of these findings could improve our understanding of neurological manifestations in AD patients with SARS-CoV-2 infection.Considering the complex mechanisms underlying the interplay between COVID-19 and AD, including the potential neuro-invasion of SARS-CoV-2 and neuroinflammation triggered via different signaling pathways, it is necessary to investigate the mechanisms through laboratory and clinical collaborations.Based on our findings, physicians could develop guidelines for risk management and physical examinations in AD patients with SARS-CoV-2 infections, such as a pathogenic examination of cognitive decline and a test of serum levels of IL-6 and TNFa.It is also essential to develop novel and effective therapeutics to prevent neurological damage and lower the risk of long-term cognitive decay.

Conclusions
Our comprehensive transcriptomic analysis provided not only possible supportive evidence of direct brain invasion but also robust evidence of neuroinflammation via the involvement of immune-related pathways after SARS-CoV-2 infection in AD patients.These findings could facilitate the investigation of the molecular mechanisms of the interplay between AD and COVID-19 and also develop effective therapeutic approaches for AD patients with COVID-19.

Figure 1 .
Figure1.The workflow for identifying critical factors in the interplay between COVID-19 and Alzheimer's disease (AD).First, the SARS-CoV-2 dataset GSE147507 and the AD dataset GSE95587 were used to find the common differentially expressed genes (DEGs between SARS-CoV-2 cells, COVID-19 patients and AD patients.DEGs were defined as |log2 fold change| ≥ 0.26 and p-value < 0.05.Then, classical enrichment analyses, including the GO and KEGG databases, were performed to assess the functional characteristics of the DEGs.Additionally, AD-related pathway enrichment scores in SARS-CoV-2 cells and patients were calculated.Furthermore, the hub genes were obtained using GeneMANIA analysis.Next, the Pearson correlation of hub genes, SARS-CoV-2 pathway-related genes, and SARS-CoV-2-related core transcription factors was performed in multiple datasets.Finally, snRNA-Seq data (GSE159812) were included to determine which cell types exhibited significant enrichment for hub genes, SARS-CoV-2 pathway-related genes, and CoV2-related core transcription factors (TFs).

Figure 1 .
Figure 1.The workflow for identifying critical factors in the interplay between COVID-19 andAlzheimer's disease (AD).First, the SARS-CoV-2 dataset GSE147507 and the AD dataset GSE95587 were used to find the common differentially expressed genes (DEGs between SARS-CoV-2 cells, COVID-19 patients and AD patients.DEGs were defined as |log2 fold change| ≥ 0.26 and p-value < 0.05.Then, classical enrichment analyses, including the GO and KEGG databases, were performed to assess the functional characteristics of the DEGs.Additionally, AD-related pathway enrichment scores in SARS-CoV-2 cells and patients were calculated.Furthermore, the hub genes were obtained using GeneMANIA analysis.Next, the Pearson correlation of hub genes, SARS-CoV-2 pathway-related genes, and SARS-CoV-2-related core transcription factors was performed in multiple datasets.Finally, snRNA-Seq data (GSE159812) were included to determine which cell types exhibited significant enrichment for hub genes, SARS-CoV-2 pathway-related genes, and CoV2-related core transcription factors (TFs).

Figure 2 .
Figure 2. DEGs are shared in AD and COVID-19 patients.(A) The volcano plot shows the D AD patients.(B) The volcano plot shows the DEGs in the lung tissues of COVID-19 patien dots are genes that are up-expressed, blue dots are genes that are down-expressed, and gr are genes that are not significantly differentially expressed.(C) The Venn diagram shows the DEGs that are up-expressed (up) and down-expressed (down) in both AD patients and CO patients.(D) GO analysis identified enriched gene clusters for up-expressed DEGs (n = 1 down-expressed DEGs (n = 136) and combined all shared up-/down-regulated DEGs (n = both AD and COVID-19 patients.

Figure 2 .
Figure 2. DEGs are shared in AD and COVID-19 patients.(A) The volcano plot shows the DEGs in AD patients.(B) The volcano plot shows the DEGs in the lung tissues of COVID-19 patients.Red dots are genes that are up-expressed, blue dots are genes that are down-expressed, and gray dots are genes that are not significantly differentially expressed.(C) The Venn diagram shows the shared DEGs that are up-expressed (up) and down-expressed (down) in both AD patients and COVID-19 patients.(D) GO analysis identified enriched gene clusters for up-expressed DEGs (n = 197) and down-expressed DEGs (n = 136) and combined all shared up-/down-regulated DEGs (n = 333) in both AD and COVID-19 patients.

Figure 3 .
Figure 3. DEGs are shared in AD and COVID-19 patients.(A) The volcano plot shows the DE SARS-CoV-2-infected Calu-3 cells.(B) The volcano plot shows the DEGs in SARS-CoV-2-inf NHBE cells.Red dots are genes that are up-expressed, blue dots are genes that are down-expre and gray dots are genes that are not significantly differentially expressed.(C) The Venn dia shows the shared DEGs that are up-expressed (up) and down-expressed (down) in both SARS-2-infected Calu-3 cells and NHBE cells.(D) GO analysis identified enriched gene clusters fo expressed DEGs (n = 364) and down-expressed DEGs (n = 122) and combined all shared up-/d regulated DEGs (n = 488) in both SARS-CoV-2-infected Calu-3 cells and NHBE cells.

Figure 3 .
Figure 3. DEGs are shared in AD and COVID-19 patients.(A) The volcano plot shows the DEGs in SARS-CoV-2-infected Calu-3 cells.(B) The volcano plot shows the DEGs in SARS-CoV-2-infected NHBE cells.Red dots are genes that are up-expressed, blue dots are genes that are down-expressed, and gray dots are genes that are not significantly differentially expressed.(C) The Venn diagram shows the shared DEGs that are up-expressed (up) and down-expressed (down) in both SARS-CoV-2-infected Calu-3 cells and NHBE cells.(D) GO analysis identified enriched gene clusters for up-expressed DEGs (n = 364) and down-expressed DEGs (n = 122) and combined all shared up-/down-regulated DEGs (n = 488) in both SARS-CoV-2-infected Calu-3 cells and NHBE cells.

3. 3 .
Changes in the Expression of Top AD DEGs in SARS-CoV-2-Infected Cells and in COVID-19 Patients

Viruses 2024, 16 , x 11 of 27 Figure 4 . 27 Figure 4 . 27 Figure 4 .
Figure 4. Changes in the expression of top AD DEGs in cells infected with SARS-CoV-2 and in COVID-19 patients (A,B) Heatmaps show the min-max normalized gene expression from DESeq2

Figure 4 .
Figure 4. Changes in the expression of top AD DEGs in cells infected with SARS-CoV-2 and in COVID-19 patients (A,B) Heatmaps show the min-max normalized gene expression from DESeq2

Figure 6 .
Figure 6.IRF7 plays a key role in pathways involved in signaling transduction in bo SARS-CoV-2 infection.(A) Venn diagram shows shared DEGs that are up-regulated (left)

Figure 6 .
Figure 6.IRF7 plays a key role in pathways involved in signaling transduction in both AD and SARS-CoV-2 infection.(A) Venn diagram shows shared DEGs that are up-regulated (left) and downregulated (right) in AD, COVID-19 patients, and SARS-CoV-2-infected Calu-3 and NHBE cells.(B) GO analysis identified enriched gene clusters for the shared up-regulated DEGs in AD, COVID-19 patients,

Viruses 2024, 16 , x 16 of 27 Figure 7 .
Figure 7. IRF7 is significantly up-regulated in response to different RNA virus infections, and the expression of ACE2 is correlated with IRF7.(A) Heatmaps show the min-max-normalized gene expression from DESeq2 changes in patients AD and COVID-19 lung and SARS-CoV-2-infected cells (Calu-3 and NHBE) for the SARS-CoV-2 and COVID-19 pathway-related genes.(B) Selected major TFs binding to the promoter regions (−1500 to 500 bps from TSS) in the SARS-CoV-2 and COVID-19 pathway-related genes predicted by MAST.The arrows indicate the direction of transcription, showing the pathway of RNA polymerase along the DNA strand for RNA synthesis.(C) Correlation of gene expression between the SARS-CoV-2 and COVID-19 pathway-related genes and 4 major TFs in patients (AD and COVID-19 lung) and SARS-CoV-2 infected cells (Calu-3 and NHBE).(D) IRF7 is up-regulated upon infections with different RNA viruses, including SARS, MERS, and HIV.(E) Correlation of gene expression with the same genes in (C) but for SARS, MERS, and HIV.(F) Expression changes in the SARS-CoV-2 and COVID-19 pathway-related genes and 4 major TFs in different cell types in human brains upon SARS-CoV-2 infection.(G) Correlation of gene expression with the same genes in (C) but for SARS-CoV-2-infected AD patients.

Figure 7 .
Figure 7. IRF7 is significantly up-regulated in response to different RNA virus infections, and the expression of ACE2 is correlated with IRF7.(A) Heatmaps show the min-max-normalized gene expression from DESeq2 changes in patients AD and COVID-19 lung and SARS-CoV-2-infected cells (Calu-3 and NHBE) for the SARS-CoV-2 and COVID-19 pathway-related genes.(B) Selected major TFs binding to the promoter regions (−1500 to 500 bps from TSS) in the SARS-CoV-2 and COVID-19 pathway-related genes predicted by MAST.The arrows indicate the direction of transcription, showing the pathway of RNA polymerase along the DNA strand for RNA synthesis.(C) Correlation of gene expression between the SARS-CoV-2 and COVID-19 pathway-related genes and 4 major TFs in patients (AD and COVID-19 lung) and SARS-CoV-2 infected cells (Calu-3 and NHBE).(D) IRF7 is up-regulated upon infections with different RNA viruses, including SARS, MERS, and HIV.(E) Correlation of gene expression with the same genes in (C) but for SARS, MERS, and HIV.(F) Expression changes in the SARS-CoV-2 and COVID-19 pathway-related genes and 4 major TFs in different cell types in human brains upon SARS-CoV-2 infection.(G) Correlation of gene expression with the same genes in (C) but for SARS-CoV-2-infected AD patients.
which is also evidenced by our previous observations of enriched pathways i based on their shared DEGs in AD patients, COVID-19 patients, and SARS-CoV-2 cells.

Figure 8 .
Figure 8.The enriched pathways in both AD and SARS-CoV-2 infection.(A-C) The s shows the pathway enrichment scores of DEGs in COVID-19 patients (A) and SARS-CoV Calu-3 (B) and NHBE cells (C) for the enriched pathways in AD patients identified by [64 top enriched pathways upon SARS-CoV-2 infection (each top 5 enriched pathway in COV tients, or SARS-CoV-2-infected Calu-3 and NHBE cells).(E) The most common enriched are shared in both AD and SARS-CoV-2 infection.

Figure 8 .
Figure 8.The enriched pathways in both AD and SARS-CoV-2 infection.(A-C) The scatter plot shows the pathway enrichment scores of DEGs in COVID-19 patients (A) and SARS-CoV-2-infected Calu-3 (B) and NHBE cells (C) for the enriched pathways in AD patients identified by [64].(D) The top enriched pathways upon SARS-CoV-2 infection (each top 5 enriched pathway in COVID-19 patients, or SARS-CoV-2-infected Calu-3 and NHBE cells).(E) The most common enriched pathways are shared in both AD and SARS-CoV-2 infection.

Table 1 .
Datasets used in this study.