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Open AccessArticle

Fetal Calf Serum Exerts an Inhibitory Effect on Replication of Duck Hepatitis A Virus Genotype 1 in Duck Embryo Fibroblast Cells

Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China
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Authors to whom correspondence should be addressed.
Viruses 2020, 12(1), 80; https://doi.org/10.3390/v12010080
Received: 11 December 2019 / Accepted: 6 January 2020 / Published: 9 January 2020
(This article belongs to the Section Antivirals & Vaccines)
Among the causative agents of duck viral hepatitis, duck hepatitis A virus genotype 1 (DHAV-1) is the most common virus reported in most outbreaks worldwide. How to propagate DHAV-1 in cell cultures efficiently remains a problem to be explored. Here, we aimed to test the effect of serum type on DHAV-1 replication in duck embryo fibroblast (DEF) cells. Comparative studies involved virus culture and passage, observation of cytopathic effect (CPE), virus quantification, and plaque formation assay. From the results of these investigations, we conclude that use of chicken serum (CS) in maintenance medium allows DHAV-1 to establish productive, cytocidal infection in DEF cells, whereas FCS exerts inhibitory effects on DHAV-1 replication, CPE development, and plaque formation. By using a neutralization test, we found that the direct action of FCS on virions is likely to play a key role in inhibiting DHAV-1 replication in DEF cells. Mechanism analyses revealed that FCS inhibits DHAV-1 replication at virus adsorption and reduces extracellular virus yields. The present work may shed light on a new perspective for antiviral agent development, and have provided a virus–host cell system for further studies on molecular mechanism involved DHAV-1 replication and pathogenesis. View Full-Text
Keywords: duck hepatitis A virus genotype 1; duck embryo fibroblast; cytocidal infection; plaque formation; fetal calf serum; inhibitory effect duck hepatitis A virus genotype 1; duck embryo fibroblast; cytocidal infection; plaque formation; fetal calf serum; inhibitory effect
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Wang, M.; Chai, L.; Liang, S.; Lv, J.; Yang, L.; Qu, S.; Jin, M.; Li, Q.; Wang, X.; Zhang, D. Fetal Calf Serum Exerts an Inhibitory Effect on Replication of Duck Hepatitis A Virus Genotype 1 in Duck Embryo Fibroblast Cells. Viruses 2020, 12, 80.

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