Increased Levels of Txa2 Induced by Dengue Virus Infection in IgM Positive Individuals Is Related to the Mild Symptoms of Dengue

The inflammatory process plays a major role in the prognosis of dengue. In this context, the eicosanoids may have considerable influence on the regulation of the Dengue virus-induced inflammatory process. To quantify the molecules involved in the cyclooxygenase and lipoxygenase pathways during Dengue virus infection, plasma levels of thromboxane A2, prostaglandin E2 and leukotriene B4; mRNA levels of thromboxane A2 synthase, prostaglandin E2 synthase, leukotriene A4 hydrolase, cyclooxygenase-2 and 5-lipoxygenase; and the levels of lipid bodies in peripheral blood leukocytes collected from IgM-positive and IgM-negative volunteers with mild dengue, and non-infected volunteers, were evaluated. Dengue virus infection increases the levels of thromboxane A2 in IgM-positive individuals as well as the amount of lipid bodies in monocytes in IgM-negative individuals. We suggest that increased levels of thromboxane A2 in IgM-positive individuals plays a protective role against the development of severe symptoms of dengue, such as vascular leakage.


Introduction
Dengue is an arthropod-borne viral disease caused by any of the four Dengue virus (DENV) serotypes (DENV-1 to -4). This virus belongs to the Flaviviridae family and is transmitted to humans through the bite of infected female mosquitoes of the genus Aedes. Dengue occurs in more than 128 countries in tropical and subtropical regions worldwide and is considered the most prevalent mosquito-borne viral disease affecting humans [1]. It is estimated that 3.9 billion people are at risk for DENV infection. In addition, an estimated 500,000 people with dengue require hospitalization each year, and approximately 2.5% of those affected will die [2].
In humans, after an incubation period of 4-8 days, DENV can induce a wide spectrum of illness, with most cases being asymptomatic or subclinical. Most patients recover after a self-limiting illness, while a small proportion of patients progresses to a severe disease state, which is primarily The aim of our study was to assess the plasma levels of TXA 2 and PGE 2 (products of the COX pathway) and LTB 4 (product of the 5-LOX pathway). As LBs are organelles involved in the synthesis of eicosanoids, we also measured the level of eicosanoids in the peripheral blood leukocytes. In addition, we quantified the gene expression levels of COX-2, thromboxane A2 synthase (TXA 2 S) and prostaglandin E 2 synthase (PGE 2 S), which are involved in the production of TXA 2 and PGE 2 , and the mRNA levels of 5-LOX and leukotriene A4 hydrolase (LTA 4 H), which are involved in the production of LTB 4 .
Here, we demonstrated that DENV infection may induce increased levels of TXA 2 in IgM-positive dengue patients as well as an increased amount of LBs in the monocytes of IgM-negative dengue patients, suggesting that the balance of TXA 2 and IgM levels plays a protective role against the development of severe symptoms of dengue, such as vascular leakage.

Ethical Aspects
This study was approved by the Ethics Committee from Instituto René Rachou/FIOCRUZ-MG (CAAE: 55135116.0.0000.5091, approved date: 18 August 2016). Participants involved in the study signed a written informed consent, and all analyzed data were anonymized.

Study Population
A cross-sectional study was conducted between March and May of 2017 in Santa Casa de Misericórdia de Santo Antônio do Monte, Santo Antônio do Monte city, Minas Gerais state, Brazil. Twenty-eight adult patients, above the age of 18, with a clinically characterized non-severe form of dengue infection were enrolled in the study. DENV infection was confirmed in the volunteers when at least one of the following tests was positive: (a) Dengue Virus NS1 ELISA (Euroimmun, Luebeck, Germany); (b) Anti-Dengue Virus ELISA (IgM) (Euroimmun); and (c) TRIOPLEX Dengue/Zika/ChikV kit (Eco Diagnóstica, Minas Gerais, Brazil). All dengue diagnostic tests were performed according to the manufacturer's instructions. Dengue patients were subsequently divided into two study groups according to the absence or presence of anti-DENV IgM in their plasma. Twelve individuals (11 women and 1 men; median age = 33 years, range = 18.0 to 85.0) comprised the IgM-positive group. Sixteen individuals (12 women and 4 men; median age = 37.5 years, range = 18.0 to 61.0) comprised the IgM-negative group. The non-infected control group was composed of twelve individuals (6 women and 6 men; median age = 32.5 years, range = 27.0 to 56.0) with no signs or symptoms of dengue and with negative results in all dengue diagnostic tests.

Eicosanoids Quantification
Plasma obtained by the centrifugation of whole blood was stored at −80 • C. All samples were thawed only once, and the levels of eicosanoids were analyzed. Levels of TXA 2 and PGE 2 were indirectly assessed using the Thromboxane B 2 ELISA Kit and Prostaglandin E Metabolite ELISA Kit, respectively. Levels of LTB 4 were directly quantified using the Leukotriene B 4 ELISA Kit. All reagents were purchased from Cayman Chemical (Ann Arbor, MI, USA), and all reactions were performed according to the manufacturer's instructions.

mRNA Quantification
Total RNA was extracted from peripheral blood leukocytes collected from DENV-infected individuals and healthy volunteers, using the PureLink™ RNA Mini Kit (ThermoFisher Scientific) following the manufacturer's protocols. The purified RNA (50 ng) was reverse transcribed to cDNA using the GoScript Reserve Transcription System with random primers (Promega, Madison, WI, USA) according to the manufacturer's instructions. The target genes were amplified and detected using TaqMan ® gene expression assays (ThermoFisher Scientific, cat. number 4,331,182-gene id: PTGS2-Hs00153133_m1, TBXAS1-Hs01022706_m1, PTGES2-Hs00228159_m1, ALOX5-Hs00167536_m1, LTA4H-Hs01075871 and ACTB-Hs_99999903_m1). Then, RNase H (2 U) (ThermoFisher Scientific) was added and the samples were incubated at 37 • C for 20 min. The RT-qPCR was performed using the StepOnePlus™ Real-Time PCR System (ThermoFisher Scientific). Reactions were performed in triplicate and included 5 µL of cDNA, 6.25 µM of each target TaqMan ® assay or human β-actin TaqMan ® assay used to normalize the gene expression data, 7.5 µL of TaqMan™ Gene Expression Master Mix (ThermoFisher Scientific) and water added to a final volume of 15 µL. Duplicates of non-template controls were included in each RT-qPCR experiment. The thermocycling conditions were as follows: initial holding (2 min at 50 • C and 10 min at 95 • C), followed by 40 cycles of 15 s at 95 • C and 1 min at 60 • C. The baseline and threshold for the cycle threshold (Ct) calculations were set automatically using the Sequence Detection Software, version 1.4 (Applied Biosystems, Waltham, MA, USA). The 2 −∆∆Ct method was used to calculate the relative quantification [22,23], using the mean of all of the NI samples as the reference value.

Dengue Virus Load Quantification
Total nucleic acids were extracted from 240 µL of plasma that was centrifuged at 20,000× g for 1.5 h at 4 • C to concentrate the viral particles. After centrifugation, the supernatant was discarded and 50 µL of buffer containing Tris Base (Inlab, São Paulo, Brazil), EDTA (Synth, São Paulo, Brazil), NaCl (Synth) and proteinase K (Qiagen, Hilden, Germany) was added. All samples were subsequently processed in the Thermal Cycler Veriti (ThermoFisher Scientific), at 56 • C for 5 min and 98 • C for 15 min. Then, the obtained nucleic acids were used to determine the DENV viral load (number of DENV RNA copies per mL of plasma), which was quantified using a LightCycler ® 96 (Roche, Mannheim, Germany) and the following primers and probe: DENV-F: 5 -AAG GAC TAG AGG TTA GAG GAG ACC C-3 , DENV-R: 5 -CGT TCT GTG CCT GGA ATG ATG-3 and DENV-Probe: 5 -/TEX615/AAC AGC ATA TTG ACG CTG GGA GAG ACC AGA/3IAbRQSp/3 [24]. Reactions were performed in duplicate and included 2.5 µL of nucleic acids, 0.25 µL of each primer, 0.1 µL of probe, 2.5 µL of TaqMan Fast Virus 1-Step Master Mix (ThermoFisher Scientific) and water added to a final volume of 10 µL. Duplicates of non-template controls were included in the RT-qPCR assays. Thermocycling conditions were as follows: initial holding (20 s at 95 • C) followed by 40 cycles of 3 s at 95 • C and 30 s at 60 • C. For quantification purposes, the sequences of the DENV amplicons (3 UTR), were cloned (pGEMT-Easy plasmid, Promega), amplified and then serially diluted to generate a standard curve, as previously described by Richardson et al. (2006) [25].

Analyses of Vacuolated Monocytes
The morphology of the monocytes was analyzed using blood smears stained with a hematology staining kit (Instant-Prov, Newprov, Paraná, Brazil), according to the manufacturer's instructions. The percentage of vacuolated monocytes was determined by counting one hundred cells using light microscopy at 1000× magnification.

Statistical Analysis
The D'Agostino and Pearson omnibus test was used to evaluate the normality of data distribution. ANOVA, followed by the Student Newman-Keuls method, was used to compare multiple normal samples. The Kruskal-Wallis test, followed by the Dunn's method, was used to compare multiple non-normal samples. The unpaired nonparametric Mann-Whitney t-test was used to evaluate the data of viral load. The Prism 6 ® software package (GraphPad Software, San Diego, CA, USA) was used for statistical tests. Differences were considered significant when a p value ≤ 0.05 was obtained.

Dengue Diagnosis
Out of 40 volunteers enrolled in this study, 12 healthy volunteers were considered non-infected by DENV, based upon negative results from RT-qPCR, ELISA NS1 and ELISA IgM tests. The 28 remaining volunteers presented dengue symptoms and were positive in at least one of the three tests used (17 were positive based upon RT-qPCR, 20 were positive based upon ELISA NS1 and 16 were positive based upon ELISA IgM). Thus, 28 volunteers were considered as DENV-infected subjects (Table 1). Due to the wide variation in days of symptoms reported by the volunteers (1-12 days of symptoms), the volunteers were divided into two groups according to the presence or absence of IgM.

Plasma from IgM-Positive Individuals Has Increased Levels of TXA 2
To evaluate the impact of DENV infection on the production of eicosanoids, we assessed the levels of TXA 2 and PGE 2 , by measuring their stable metabolites, and LTB4 in the plasma from DENV-infected individuals. As shown in Figure 1, the IgM-positive group displayed higher plasma levels of TXA 2 compared with the non-infected group (555.9 pg/mL versus 155.3 pg/mL; p < 0.05) and the IgM-negative group (555.9 pg/mL versus 141.3 pg/mL; p < 0.01). The plasma levels of PGE 2 and LTB 4 were not significantly altered by the DENV infection.

Plasma from IgM-Positive Individuals Has Increased Levels of TXA2
To evaluate the impact of DENV infection on the production of eicosanoids, we assessed the levels of TXA2 and PGE2, by measuring their stable metabolites, and LTB4 in the plasma from DENVinfected individuals. As shown in Figure 1, the IgM-positive group displayed higher plasma levels of TXA2 compared with the non-infected group (555.9 pg/mL versus 155.3 pg/mL; p < 0.05) and the IgM-negative group (555.9 pg/mL versus 141.3 pg/mL; p < 0.01). The plasma levels of PGE2 and LTB4 were not significantly altered by the DENV infection.

Monocytes from IgM-Negative Individuals Displayed Increased Levels of LBs
To investigate the effect of DENV infection on the levels of LBs in the peripheral blood leukocytes, we measured the median fluorescence intensity (MFI) of Bodipy 493/503, a green fluorescent dye that binds to the neutral lipids in LBs. Figure 2A shows that the MFI of Bodipy 493/503 in CD16 + (15,007 versus 9238) and CD16 − (13,824 versus 8806) monocytes from the IgM-negative group was higher compared with the non-infected group (p < 0.05). In contrast, there was no difference in the MFI of Bodipy 493/503 when comparing subpopulations of monocytes from the non-infected and IgM-negative groups. The gating strategy used to analyze the monocytes is demonstrated in Figure 2B. DENV infection did not alter the MFI of Bodipy 493/503 in neutrophils, eosinophils, lymphocytes, NK and NK T cells [26].

Monocytes from IgM-Negative Individuals Displayed Increased Levels of LBs
To investigate the effect of DENV infection on the levels of LBs in the peripheral blood leukocytes, we measured the median fluorescence intensity (MFI) of Bodipy 493/503, a green fluorescent dye that binds to the neutral lipids in LBs. Figure 2A shows that the MFI of Bodipy 493/503 in CD16 + (15,007 versus 9238) and CD16 − (13,824 versus 8806) monocytes from the IgM-negative group was higher compared with the non-infected group (p < 0.05). In contrast, there was no difference in the MFI of Bodipy 493/503 when comparing subpopulations of monocytes from the noninfected and IgM-negative groups. The gating strategy used to analyze the monocytes is demonstrated in Figure 2B. DENV infection did not alter the MFI of Bodipy 493/503 in neutrophils, eosinophils, lymphocytes, NK and NK T cells (data not shown) [26].

Expression Levels of COX-2 and TXA 2 S Genes Were Lower in Leukocytes from IgM-Positive Individuals
The effect of DENV infection on the mRNA levels of genes coding for eicosanoid-forming enzymes, such as COX-2, TXA 2 S and PGE 2 S (COX pathway), and 5-LOX and LTA 4 H (5-LOX pathway), in peripheral blood leukocytes collected from DENV-infected individuals was assessed by qRT-PCR. We observed that the IgM-positive group presented lower levels of mRNA coding for the COX-2 gene (0.22 versus 0.58; p < 0.05) and the TXA 2 S gene (0.33 versus 0.56; p < 0.05) when compared to the non-infected group. The gene expression levels of PGE 2 S, 5-LOX and LTA 4 H were not altered by the DENV infection (Figure 3).

Expression Levels of COX-2 and TXA2S Genes Were Lower in Leukocytes from IgM-Positive Individuals
The effect of DENV infection on the mRNA levels of genes coding for eicosanoid-forming enzymes, such as COX-2, TXA2S and PGE2S (COX pathway), and 5-LOX and LTA4H (5-LOX pathway), in peripheral blood leukocytes collected from DENV-infected individuals was assessed by qRT-PCR. We observed that the IgM-positive group presented lower levels of mRNA coding for the COX-2 gene (0.22 versus 0.58; p < 0.05) and the TXA2S gene (0.33 versus 0.56; p < 0.05) when compared to the non-infected group. The gene expression levels of PGE2S, 5-LOX and LTA4H were not altered by the DENV infection (Figure 3).

The Dengue virus Load was Higher in Plasma from IgM-Negative Patients
To determine the viral load in the plasma of each dengue patient, we performed qRT-PCR. As shown in Figure 4A, an increase in the number of viral copies was observed in the IgM-negative group compared with the IgM-positive group (13,335 copies /mL versus 0.0 copies/mL; p < 0.001).

The Dengue Virus Load Was Higher in Plasma from IgM-Negative Patients
To determine the viral load in the plasma of each dengue patient, we performed qRT-PCR. As shown in Figure 4A, an increase in the number of viral copies was observed in the IgM-negative group compared with the IgM-positive group (13,335 copies /mL versus 0.0 copies/mL; p < 0.001).

Expression Levels of COX-2 and TXA2S Genes Were Lower in Leukocytes from IgM-Positive Individuals
The effect of DENV infection on the mRNA levels of genes coding for eicosanoid-forming enzymes, such as COX-2, TXA2S and PGE2S (COX pathway), and 5-LOX and LTA4H (5-LOX pathway), in peripheral blood leukocytes collected from DENV-infected individuals was assessed by qRT-PCR. We observed that the IgM-positive group presented lower levels of mRNA coding for the COX-2 gene (0.22 versus 0.58; p < 0.05) and the TXA2S gene (0.33 versus 0.56; p < 0.05) when compared to the non-infected group. The gene expression levels of PGE2S, 5-LOX and LTA4H were not altered by the DENV infection ( Figure 3).

The Dengue virus Load was Higher in Plasma from IgM-Negative Patients
To determine the viral load in the plasma of each dengue patient, we performed qRT-PCR. As shown in Figure 4A, an increase in the number of viral copies was observed in the IgM-negative group compared with the IgM-positive group (13,335 copies /mL versus 0.0 copies/mL; p < 0.001).

An Increased Percentage of Vacuolated Monocytes was Observed in IgM-Negative Individuals
To investigate whether DENV induces cytoplasmic vacuolization in monocytes, we assessed the percentage of vacuolated monocytes in blood smears from dengue patients. We observed that the IgM-negative group presented a percentage of vacuolated monocytes significantly higher than that of the non-infected group (21.6% versus 7.2%; p < 0.05, Figure 4B). An example of a vacuolated monocyte in the blood from a DENV-infected individual is presented in Figure 4C.

Discussion
In this work, we aimed to evaluate whether DENV infection affects the synthesis of eicosanoids mediated by the COX and 5-LOX enzymes. Thus, the levels of TXA2, PGE2 and LTB4, as well as the levels of mRNA coding for the TXA2S, PGE2S and LTA4H, COX-2 and 5-LOX genes were assessed in blood collected from 28 DENV-infected individuals with mild symptoms and from non-infected volunteers. Based on the chronology of the humoral response to dengue [1,27], the infected individuals were subdivided into the IgM-negative group and IgM-positive group, representing the early and late stages of the acute phase of dengue, respectively.
Our data revealed increased levels of TXA2 in IgM-positive dengue patients. Indeed, TXA2 is one of the most potent eicosanoids involved in vasoconstriction and platelet aggregation [28]. Therefore, the increased levels of TXA2, associated with unaltered levels of the vasodilators PGE2 and LTB4, may have contributed to the absence of severe symptoms of dengue, such as vascular extravasation, in the individuals involved in our study. In fact, it has been suggested that TXA2 may play a protective role in dengue, since decreased levels of TXA2 in the blood of patients with dengue have been associated with the development of hypovolemic shock signals [9]. Moreover, PGE2 seems to play a deleterious

An Increased Percentage of Vacuolated Monocytes was Observed in IgM-Negative Individuals
To investigate whether DENV induces cytoplasmic vacuolization in monocytes, we assessed the percentage of vacuolated monocytes in blood smears from dengue patients. We observed that the IgM-negative group presented a percentage of vacuolated monocytes significantly higher than that of the non-infected group (21.6% versus 7.2%; p < 0.05, Figure 4B). An example of a vacuolated monocyte in the blood from a DENV-infected individual is presented in Figure 4C.

Discussion
In this work, we aimed to evaluate whether DENV infection affects the synthesis of eicosanoids mediated by the COX and 5-LOX enzymes. Thus, the levels of TXA2, PGE2 and LTB4, as well as the levels of mRNA coding for the TXA2S, PGE2S and LTA4H, COX-2 and 5-LOX genes were assessed in blood collected from 28 DENV-infected individuals with mild symptoms and from non-infected volunteers. Based on the chronology of the humoral response to dengue [1,27], the infected individuals were subdivided into the IgM-negative group and IgM-positive group, representing the early and late stages of the acute phase of dengue, respectively.
Our data revealed increased levels of TXA2 in IgM-positive dengue patients. Indeed, TXA2 is one of the most potent eicosanoids involved in vasoconstriction and platelet aggregation [28]. Therefore, the increased levels of TXA2, associated with unaltered levels of the vasodilators PGE2 and LTB4, may have contributed to the absence of severe symptoms of dengue, such as vascular extravasation, in the individuals involved in our study. In fact, it has been suggested that TXA2 may play a protective role in dengue, since decreased levels of TXA2 in the blood of patients with dengue have been associated with the development of hypovolemic shock signals [9]. Moreover, PGE2 seems to play a deleterious = indicate the vacuoles.

An Increased Percentage of Vacuolated Monocytes Was Observed in IgM-Negative Individuals
To investigate whether DENV induces cytoplasmic vacuolization in monocytes, we assessed the percentage of vacuolated monocytes in blood smears from dengue patients. We observed that the IgM-negative group presented a percentage of vacuolated monocytes significantly higher than that of the non-infected group (21.6% versus 7.2%; p < 0.05, Figure 4B). An example of a vacuolated monocyte in the blood from a DENV-infected individual is presented in Figure 4C.

Discussion
In this work, we aimed to evaluate whether DENV infection affects the synthesis of eicosanoids mediated by the COX and 5-LOX enzymes. Thus, the levels of TXA 2 , PGE 2 and LTB 4 , as well as the levels of mRNA coding for the TXA 2 S, PGE 2 S and LTA 4 H, COX-2 and 5-LOX genes were assessed in blood collected from 28 DENV-infected individuals with mild symptoms and from non-infected volunteers. Based on the chronology of the humoral response to dengue [1,27], the infected individuals were subdivided into the IgM-negative group and IgM-positive group, representing the early and late stages of the acute phase of dengue, respectively.
Our data revealed increased levels of TXA 2 in IgM-positive dengue patients. Indeed, TXA 2 is one of the most potent eicosanoids involved in vasoconstriction and platelet aggregation [28]. Therefore, the increased levels of TXA 2 , associated with unaltered levels of the vasodilators PGE 2 and LTB 4 , may have contributed to the absence of severe symptoms of dengue, such as vascular extravasation, in the individuals involved in our study. In fact, it has been suggested that TXA 2 may play a protective role in dengue, since decreased levels of TXA 2 in the blood of patients with dengue have been associated with the development of hypovolemic shock signals [9]. Moreover, PGE 2 seems to play a deleterious role in dengue prognosis, since increased plasma levels of PGE 2 were observed in a group of 11 individuals with dengue who developed vascular extravasation, as reported by Lin et al. (2017) [11]. PGE 2 and TXA 2 are synthesized from AA metabolism by the action of two cyclooxygenase isoforms, known as cyclooxygenase-1 (with constitutive expression) and COX-2 (inducible), to generate PGH 2 . Then, PGH 2 can be converted by the enzymes PGE 2 S or TXA 2 S into PGE 2 and TXA 2 , respectively. TXA 2 has a short half-life and is quickly converted to the stable but physiologically inactive metabolite thromboxane B 2 [20]. The production of LTB 4 begins when AA is metabolized by 5-LOX to form an unstable epoxy intermediate, which undergoes hydrolysis to generate leukotriene A 4 . Then, leukotriene A 4 is converted to LTB 4 by the action of the enzyme LTA 4 H [29]. TXA 2 [30], PGE 2 , [31] and LTB 4 [32] can be produced by various cells, including leukocytes. Therefore, to verify whether leukocytes were involved with the increased levels of TXA 2 observed after DENV infection and IgM production, we first quantified the LBs in the main peripheral blood leukocyte populations, since these organelles are the main sites of intracellular eicosanoid production [14].
We observed that the subpopulations of CD16 − and CD16 + monocytes of the IgM-negative group displayed higher levels of LBs compared with the non-infected group, whereas the other leucocytes presented levels of LBs similar to those of the uninfected group, independent of presence or absence of IgM [26]. In fact, Samsa et al. (2009) [33] observed that BHK cells presented an increased number of LBs after in vitro infection by DENV-2. The authors postulated that the LBs sequester DENV capsid proteins and participate in the encapsidation of the DENV genome [33]. In addition, Carvalho et al., (2012) [16] confirmed the interaction between viral capsid protein and the protein perilipin 3 (TIP47) from LBs [16]. Moreover, Assunção-Miranda et al. (2010) [34] demonstrated that the peripheral blood leukocytes from dengue patients displayed increased amounts of LBs. However, in their study, the specific population of leukocytes that showed the increase of these organelles was not discriminated [34]. Our study, therefore, is the first to identify monocytes as the leukocytes that display altered levels of LBs in patients infected by DENV.
Thus, in this study, we suggest that the increase of the LB levels observed in monocytes of the IgM-negative group, despite not being associated with alterations in the plasma levels of eicosanoids or the mRNA levels of genes involved in eicosanoid-forming enzymes in leukocytes, is due to DENV infection and replication, since this increase was observed in the subpopulations of both positive and negative CD16 monocytes, both of which are permissive cells to viral infection and replication [35]. Our hypothesis is supported by the fact that the IgM-negative dengue patients also presented high DENV loads in the bloodstream. Moreover, the IgM-negative group presented a higher percentage of vacuolated monocytes in their blood, compared with the IgM-positive group. Corroborating our results, Mosquera et al. (2005) [18] also observed high vacuolization in human monocytes after in vitro infection with DENV-2 [18].
Because gene expression levels of COX-2 and TXA 2 S, which are involved in TXA 2 production, were lower in the IgM-positive group, we hypothesize that other sources of TXA 2 , such as platelets and/or vascular endothelial cells, both of which are activated after DENV infection [36,37], may have contributed to the elevated TXA 2 plasma levels of the IgM-positive group. In fact, platelets are the major source of TXA 2 in vivo [30], and it has been observed that endothelial cells are also able to synthesize TXA 2 [38]. In this context, a recent study conducted by Lin et al. (2017) [11] reported an increase in COX-2 mRNA levels in whole blood of dengue patients who developed severe illness, whereas the patients involved in our study presented only mild symptoms of dengue [11]. Red blood cells and platelets may also express COX-2 [39,40], which may have contributed to the increased levels of COX-2 mRNA observed by the authors. Moreover, an in vitro study conducted by Loke et al. [10] in 2013 showed an increase in 5-LOX expression in neutrophils stimulated by DENV-2, which was followed by LTB 4 production [10]. This contrasting result can be explained by the short time of exposure (7 h) and high viral load used in their study, which does not reflect the natural infection and, consequently, the progression of the disease or the immune response. Our work is the first that describes, ex vivo, the influence of DENV infection on the levels of TXA 2 S, PGE 2 S and LTA 4 H mRNA.
Here, we propose a model for the role of LB and TXA 2 in dengue prognosis, as presented in Figure 5. During the time course of DENV infection, the virus triggers morphological and functional changes in monocytes, increasing the amount of LBs and vacuoles therein, which may be optimal sites of viral replication. Further, a high viral load and an unbalanced immune response could lead to the onset of dengue symptoms, including vascular damage with plasma leakage and bleeding. However, after seroconversion, characterized by the production of IgM, a high amount of TXA 2 is produced, putatively by platelets and/or endothelial cells, which induces vasoconstriction and platelet aggregation, thereby preventing the intense vascular leakage and bleeding observed in severe dengue cases.
Viruses 2018, 10, x FOR PEER REVIEW 11 of 14 changes in monocytes, increasing the amount of LBs and vacuoles therein, which may be optimal sites of viral replication. Further, a high viral load and an unbalanced immune response could lead to the onset of dengue symptoms, including vascular damage with plasma leakage and bleeding. However, after seroconversion, characterized by the production of IgM, a high amount of TXA2 is produced, putatively by platelets and/or endothelial cells, which induces vasoconstriction and platelet aggregation, thereby preventing the intense vascular leakage and bleeding observed in severe dengue cases. Taken together, our data led us to infer that during the early stages of the acute phase of dengue, when any or low levels of IgM can be detected, there is an increase in the levels of LBs in monocytes. Given that these cells are susceptible to DENV infection and the time course of infection coincides with the highest viral load but no concomitant changes were observed in the levels of mRNA coding for the enzymes involved in eicosanoids synthesis, we hypothesized that in the early stage of the disease, LBs are related to the replication of DENV in monocytes. We also concluded that the activation of the eicosanoid synthesis pathways occurs exclusively at the beginning of the defervescence of the disease, characterized by the production of TXA2. Since the individuals analyzed in our study did not develop severe dengue and TXA2 is a potent vasoconstrictor and platelet activator, we believe that this lipid mediator may play an important role in limiting the development of vascular leakage during DENV infection. More studies are warranted to investigate the role of LBs as well as the COX-and 5-LOX-mediated eicosanoid synthesis pathways during DENV infection. Finally, our data indicate that LBs could be interesting therapeutic targets to control viral replication during DENV infection and that eicosanoids may serve as important biomarkers of dengue prognosis. vacuoles, which may be optimal sites of viral replication (*). Further, a high viral load and an unbalanced immune response could lead to the onset of dengue symptoms, including vascular damage with plasma leakage and bleeding (**). However, after seroconversion, characterized by the production of IgM, high levels of TXA 2 are produced, putatively by platelets and/or endothelial cells, which may trigger vasoconstriction and platelet aggregation, preventing the intense vascular leakage and bleeding observed in severe dengue cases (***).
Taken together, our data led us to infer that during the early stages of the acute phase of dengue, when any or low levels of IgM can be detected, there is an increase in the levels of LBs in monocytes. Given that these cells are susceptible to DENV infection and the time course of infection coincides with the highest viral load but no concomitant changes were observed in the levels of mRNA coding for the enzymes involved in eicosanoids synthesis, we hypothesized that in the early stage of the disease, LBs are related to the replication of DENV in monocytes. We also concluded that the activation of the eicosanoid synthesis pathways occurs exclusively at the beginning of the defervescence of the disease, characterized by the production of TXA 2 . Since the individuals analyzed in our study did not develop severe dengue and TXA 2 is a potent vasoconstrictor and platelet activator, we believe that this lipid mediator may play an important role in limiting the development of vascular leakage during DENV infection. More studies are warranted to investigate the role of LBs as well as the COX-and 5-LOX-mediated eicosanoid synthesis pathways during DENV infection. Finally, our data indicate that LBs could be interesting therapeutic targets to control viral replication during DENV infection and that eicosanoids may serve as important biomarkers of dengue prognosis.