Bowman‒Birk Inhibitor Suppresses Herpes Simplex Virus Type 2 Infection of Human Cervical Epithelial Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Lines and Virus
2.2. BBI
2.3. Reagents
2.4. Cell Viability Assay
2.5. In Vitro Antiviral Assay
2.6. RNA Extraction and Real-Time PCR
2.7. Western Blot Analysis
2.8. Statistical Analysis
3. Results
3.1. BBI Inhibits HSV-2 Infection of End1/E6E7 Cells
3.2. BBI Suppresses HSV-2 Gene Expression
3.3. BBI Activates the JAK/STAT Signaling Pathway
3.4. BBI Inhibits the Cellular UPS
3.5. BBI Inhibits HSV-2-Induced Activation of NF-κB and MAPK
3.6. BBI Suppresses HSV-2-Induced Downregulation of Tight Junction Proteins
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Gene Name | Sequence | |
---|---|---|
Forward (5′-3′) | Reverse (5′-3′) | |
GAPDH | GGTGGTCTCCTCTGACTTCAACA | GTTGCTGTAGCCAAATTCGTTGT |
IFN-α | TTTCTCCTGCCTGAAGAACAG | GCTCATGATTTCTGCTCTGACA |
IFN-β | GCCGCATTGACCATCTATGAGA | GAGATCTTCAGTTTCGGAGGTAAC |
IFN-λ1 | CTTCCAAGCCCACCCCAACT | GGCCTCCAGGACCTTCAGC |
IFN-λ2/3 | TTTAAGAGGGCCAAAGATGC | TGGGCTGAGGCTGGATACAG |
IRF3 | ACCAGCCGTGGACCAAGAG | TACCAAGGCCCTGAGGCAC |
IRF7 | TGGTCCTGGTGAAGCTGGAA | GATGTCGTCATAGAGGCTGTTGG |
HSV-2 ICP0 | GTGCATGAAGACCTGGATTCC | GGTCACGCCCACTATCAGGTA |
HSV-2 ICP27 | TTCTGCGATCCATATCCGAGC | AAACGGCATCCCGCCAAA |
HSV-2 ICP8 | AGGACATAGAGACCATCGCGTTCA | TGGCCAGTTCGCTCACGTTATT |
HSV-2 gC | AAATCCGATGCCGGTTTCCCAA | TTACCATCACCTCCTCTAAGCTAGGC |
HSV-2 gD | ATCCGAACGCAGCCCCGC | TCTCCGTCCAGTCGTTTAT |
HSV-2 DNA polymerase | GCTCGAGTGCGAAAAAACGTTC | CGGGGCGCTCGGCTAAC |
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Liu, Y.; Xu, X.-Q.; Zhang, B.; Gu, J.; Meng, F.-Z.; Liu, H.; Zhou, L.; Wang, X.; Hou, W.; Ho, W.-Z. Bowman‒Birk Inhibitor Suppresses Herpes Simplex Virus Type 2 Infection of Human Cervical Epithelial Cells. Viruses 2018, 10, 557. https://doi.org/10.3390/v10100557
Liu Y, Xu X-Q, Zhang B, Gu J, Meng F-Z, Liu H, Zhou L, Wang X, Hou W, Ho W-Z. Bowman‒Birk Inhibitor Suppresses Herpes Simplex Virus Type 2 Infection of Human Cervical Epithelial Cells. Viruses. 2018; 10(10):557. https://doi.org/10.3390/v10100557
Chicago/Turabian StyleLiu, Yu, Xi-Qiu Xu, Biao Zhang, Jun Gu, Feng-Zhen Meng, Hang Liu, Li Zhou, Xu Wang, Wei Hou, and Wen-Zhe Ho. 2018. "Bowman‒Birk Inhibitor Suppresses Herpes Simplex Virus Type 2 Infection of Human Cervical Epithelial Cells" Viruses 10, no. 10: 557. https://doi.org/10.3390/v10100557