Arthrinium arundinis , a Novel Causal Agent of Moso Bamboo ( Phyllostachys edulis ) Culm Rhomboid Rot and Its Sensitivity to Fungicides

: Culm rhomboid rot is a new disease of Moso bamboo ( Phyllostachys edulis ) and mainly occurs on Moso bamboo grown at an altitude above 800 m. The typical symptoms start with black spots and irregular shapes, which expand vertically into an elongated, fusiform, or rhomboid shape up to 15 cm in length. Eleven fungal isolates were isolated from infected tissue, and the isolate BBB1 was conﬁrmed as the pathogen following Koch’s postulates in vitro and in vivo. Regarding morphology, BBB1 was identiﬁed as Arthrinium sp.–conidia single-celled, brown to dark, smooth, globose to subglobose, with an equatorial slit, 5.0–9.3 × 3.1–7.3 µ m in size. The conidiophores were reduced to conidiogenous cells, pale brown to dark, smooth, and ampulliform. Phylogenetic analysis based on ITS- TEF1-α -TUB2 concatenate sequences identiﬁed BBB1 as A. arundinis . Furthermore, the sensitivity of the pathogen against six common fungicides was investigated with mycelial growth assays. Prochloraz exhibited the most potent inhibition with an EC 50 value of 0.019 mg/L; another effective fungicide was difenoconazole, with an EC 50 value of 0.195 mg/L. This is the ﬁrst report of A. arundinis causing culm rhomboid rot of Moso bamboo in China; the results provide a basis for managing Moso bamboo culm rhomboid rot.


Introduction
Moso bamboo (Phyllostachys edulis), an economically important species of the subfamily Bambusoideae of the family Poaceae, is a temperate species of giant timber bamboo native to China that is now widely distributed in East and Southeast Asia, Africa, and the USA [1,2].A grown-up Moso bamboo culm can reach a height of up to 28 m and a diameter of up to 20 cm, with very high mechanical strength, and its physical properties boast an average breaking tenacity more than three times that of cotton, wool, rayon, or polyester.These extraordinary properties of culms qualify Moso bamboo as industrial bamboo [2].In addition, Moso bamboo plays an important role in controlling soil erosion and other climate regulation because of its rapid growth [3].As an important part of forest resources in southern China, Moso bamboo covers more than 4 million ha 2 in subtropical areas [4].However, intensive production makes Moso bamboo susceptible to infectious diseases.Several Moso bamboo diseases, such as dieback blight (Ceratosphaeria phyllostachydis), foot rot (Arthrinium phaeospermum), leaf spot (Alternaria alternata), sooty blotch (Meliola phyllostachydis), root rot (Fusarium acuminatum), black spot (Coccostroma arundinariae), and leaf rust (Puccinia phyllostachydis), have been found in China and have caused great losses [5].
In 2015, we found a new disease of Moso bamboo in Guangze County (117 • 20 29.75 E, 27 • 32 48.45 N), Nanping City, Fujian Province, China, named culm rhomboid rot.The typical symptoms commonly appear on aged (five-year-old and above) Moso bamboo with black spots on the surface of the culm, and with the interior of these spots turning black.A few years later, in 2018, the same disease was discovered in Huajiashan Moso Bamboo Farm (117 • 50 19.52E, 27 • 11 58.12N), Jianyang District, and then in Wuyishan County (117 • 39 24.11 E, 27 • 42 1.37 N), Nanping City in 2020.According to our survey, the disease infected approximately 30% of the Moso bamboo in Huajiashan Moso Bamboo Farm in 2018, and the incidence reached 36% in 2020 (unpublished survey data).To our knowledge, there has been no report of such a disease in Moso bamboo (P.edulis) in southeast China.A similar symptom record has been reported for fishscale bamboo (P.heteroclada) in Sichuan Province (103 • 3 0.41 E, 30 • 1 5.85 N), China, and three fungal species, Parakarstenia phyllostachydis, Neostagonosporella sichuanensis, and Podonectria sichuanensis, were collected and identified from the lesion tissues; nevertheless, none of them were tested in terms of their pathogenicity toward fishscale bamboo [6][7][8].In addition, Fusarium oxysporum was considered the pathogen of rhomboid rot in Moso bamboo in Jiangxi Province (114 • 7 4.48 E, 26 • 39 18.29 N), China [9].However, the F. oxysporum we isolated from the diseased Moso bamboo culms could not reproduce rhomboid rot symptoms.Thus, there is confusion in the research concerning the pathogen of the culm rhomboid rot disease of Moso bamboo.
A. arundinis can be found in various plant hosts worldwide, several of which are plant pathogens.A. arundinis causes leaf edge spots on peaches and leaf blight in tea plants in China [25,26]; it also causes kernel blight on barley in the USA and the leaf spots on rosemary in Iran [27,28].Reports on Arthrinium-related bamboo diseases have begun emerging quite recently, and some new species and/or new bamboo hosts have been reported [5,17,18,20,21].A. arundinis causes brown culm streaks of early spring bamboo shoots (P.praecox), with small yellow or sandy beige cycle round spots (2 mm) appearing initially, then developing into brown streaks (10 to 15 × 1 to 2 mm) [17].
The methods of managing bamboo diseases mainly include removing the infected culms and spraying fungicides.However, there are few studies on fungicide susceptibility screening for the Arthrinium pathogens of bamboo.The objectives of this study were: (i) To isolate and confirm the pathogen of Moso bamboo culm rhomboid rot following Koch's postulates; (ii) to identify the species of the pathogen of Moso bamboo culm rhomboid rot through morphological characteristics and gene phylogenetic analysis; and (iii) to determine the sensitivity of this pathogen against six common fungicides.The results of this study will provide a basis for the identification and management of this disease.

Field survey and Fungal Isolation
The incidence of culm rhomboid rot in Moso bamboo was surveyed in Huajiashan Moso Bamboo Farm (117 For pathogen isolation, the specimens were cut into small pieces (5 × 5 mm) from lesion margins, and the small pieces were surface-disinfected by submerging them into 2% sodium hypochlorite solution for 2 min and then in 75% ethanol for 1 min, followed by washing thrice with sterile water, consecutively.The sterilized pieces were wiped dry with sterilized filter paper and then placed into Petri dishes containing PDA (five pieces per dish) amended with 50 µg/mL of ampicillin.The Petri dishes were incubated at 25 • C with a 12 h light/dark cycle.After two days, hyphal tips from the leading edge fungal colonies that grew from tissues were transferred to fresh PDA to obtain pure cultures, and the pure cultures were maintained at 25 • C.

Pathogenicity Assay 2.2.1. Inoculation under Laboratory Conditions
The pathogenicity of all of the isolates was assayed on culm chips of Moso bamboo in vitro.The culms of the Moso bamboo were cut and split into pieces (5 × 10 cm).Then, the pieces were sterilized by spraying them with 75% ethanol and wounded with sterile drills.Mycelial plugs (5 mm diameter) from the edge of each isolate colony were placed on the artificial wounds of the Moso bamboo pieces.The control Moso bamboo pieces were inoculated using PDA plugs without fungus.All of the treatments were placed in moisture chambers containing wet paper towels to retain their moisture and were kept at 25 • C with a 12 h light/dark cycle.The symptoms were recorded over 10 days.Three replicates were repeated for each treatment, and the experiment was conducted twice.Isolates causing symptoms similar to those in the wild were selected for pathogenicity tests under field conditions.

Inoculation under Field Conditions
The isolate BBB1, respective to the suspected causal agent, was used to confirm the pathogenicity under field conditions.One-year-old and five-year-old healthy Moso bamboos without disease symptoms in nature in the bamboo garden of Fujian Agriculture and Forestry University were used for artificial inoculation.The culm surfaces were sterilized with 75% ethanol and wounded using sterilized drills.The mycelial plugs of BBB1 were obtained and inoculated on the culms following the method described above.After this, sterilized absorbent cotton moistened with sterile water was used to cover the wounds, and then the absorbent cotton was sealed with plastic wrap.Control culms were treated with pure PDA plugs following the same procedure.Developing rot culms were collected for the re-isolation of the fungus for pathogen verification and the completion of Koch's postulates [29].Each treatment set three replicates and the experiment was conducted twice from May to August 2021 (temperature: 22-29 • C, average precipitation: 212 mm).

Morphological Characterization
For morphological identification, the purified isolate BBB1 was incubated on PDA for 4 days at 25 • C. The characteristics of the colony and the diameter, color, and texture were measured and recorded daily, and the growth rate of the mycelia was calculated using the following formula: Growth rate (cm/day) = 1/2 (Dia2 − Dia1), where Dia2 is the colony diameter after culturing for 24 h in Dia1.The asexual reproduction characters were observed based on cultures on malt extract agar (MEA; malt extract 20 g and 15 g agar) and according to the synoptic keys proposed for Arthrinium species identification [11].The shape and size of the microscopic structures were observed and measured using a Nikon microscope (ECLIPSE NI-U, Nikon, Tokyo, Japan) connected to a Nikon DS-Ri2 camera and software.At least 50 conidiogenous cells and conidia were measured to calculate the mean size.

Molecular and Phylogenetic Analysis
The genomic DNA of the isolate BBB1 was extracted using an SP Fungal DNA Kit (D5542, Omega Bio-tek, Norcross, GA, USA).The ITS was amplified using the primer ITS1/ITS4 [30].The partial sequence of TEF1-α and TUB2 were amplified following the method of Carbone and Kohn and Glass and Donaldson [31,32], respectively.PCR reactions were performed in a C1000 Touch Thermal Cycler (Bio-Rad, USA) with a total volume of 50 µL.Amplicons were sequenced by Sangon Biotech Company (Shanghai, China), and DNA sequences were aligned and edited using MEGA 7.0 software [33].Twenty-four strains involving 10 species of Arthinium were used for the phylogenetic analyses, and their ITS, TEF1-α, and TUB2 sequences were downloaded from GenBank and are listed in Table 1.Phylogenetic analyses were conducted in the MEGA 7.0 software using the maximum likelihood (ML) method based on the concatenated ITS-TEF1-α-TUB2 sequences [34].For ML analysis, the branch stability was determined by 1000 bootstrap replicates, and Nigrospora bambusa was used as the outgroup.

Inhibition rate (%) =
Diameter of control fungal colony − Diameter of treated fungal colony Diameter of control fungal colony × 100% The median effective concentration (EC 50 ) value was calculated by linear regressions of the inhibition rates of mycelial growth (dependent variable, Y) versus the log 10 transformation of the fungicide concentrations (independent variable, X).

Symptoms Description, Fungal Isolation, and Pathogenicity
From the field survey, rhomboid rot culms were widespread in the Moso bamboo forest cultured at an altitude above 800 m.Symptoms developed on the culm of the Moso bamboo, especially on the middle and lower culms of aged (five-year-old and above) Moso bamboo.The typical symptoms started with black spots and irregular shapes that expanded vertically into an elongate, fusiform, or rhomboid shape, with diseased spots reaching up to 15 cm in length (Figure 1A,B,D).Necrosis did not only occur on the surface, but also invaded the culm wall and entered the inner cavity of the culm, and the infected area of the bamboo cavity turned black and became necrotic (Figure 1E,F).In addition, macroscopic pink fungal ascomata appeared, surrounding the spots (Figure 1B-D).Diseased spots can spread over the whole Moso bamboo culm when the disease becomes severe, causing the entire Moso bamboo to wilt.
used to determine the sensitivity of the isolate BBB1 in vitro.Stock solutions of the active ingredient at 10 3 mg/L were created by dissolving the fungicides in sterile distilled water and adding it into PDA to obtain a range of final concentrations, as follows: difenocona zole: 0.09375, 0.1875, 0.375, 0.75, and 1.5 mg/L; hymexazol: 50, 100, 150, 200, and 250 mg/L iprodione: 4, 5, 6, 7, and 8 mg/L; mancozeb: 100, 200, 300, 400, and 500 mg/L; prochloraz 0.00625, 0.0125, 0.025, 0.05, and 0.1 mg/L; propiconazole: 0.625, 1.25, 2.5, 5, and 10 mg/L PDA without fungicide was used as the negative control.PDA plugs (5 mm in diameter were removed from the edge of four-day-old cultures and transferred to the center of the PDA plates amended with the fungicides and negative control.Three replicates of each treatment were conducted.The colony diameter was measured in two perpendicular di rections after incubation for 4 days at 25 °C.The experiment was conducted twice inde pendently.The inhibition rate of mycelial growth related to the control was calculated fo all the fungicide concentrations.

Inhibition rate (%) =
Diameter of control fungal colony − Diameter of treated fungal colony Diameter of control fungal colony

× 100%
The median effective concentration (EC50) value was calculated by linear regressions of the inhibition rates of mycelial growth (dependent variable, Y) versus the log10 trans formation of the fungicide concentrations (independent variable, X).

Symptoms Description, Fungal Isolation, and Pathogenicity
From the field survey, rhomboid rot culms were widespread in the Moso bamboo forest cultured at an altitude above 800 m.Symptoms developed on the culm of the Moso bamboo, especially on the middle and lower culms of aged (five-year-old and above Moso bamboo.The typical symptoms started with black spots and irregular shapes tha expanded vertically into an elongate, fusiform, or rhomboid shape, with diseased spots reaching up to 15 cm in length (Figure 1A, B, D).Necrosis did not only occur on the sur face, but also invaded the culm wall and entered the inner cavity of the culm, and the infected area of the bamboo cavity turned black and became necrotic (Figure 1E, F).In addition, macroscopic pink fungal ascomata appeared, surrounding the spots (Figure 1B,C,D).Diseased spots can spread over the whole Moso bamboo culm when the disease becomes severe, causing the entire Moso bamboo to wilt.Eleven fungal isolates were isolated from the infected tissue specimens, identified as six different species (Figure S1) according to the colony characteristics.The fungus with pink ascomata could not grow on PDA and was identified as Podonectria according to Figure S2 and Yang et al. [6].All of the isolates and Podonectria ascospores were inoculated on culm pieces in the laboratory, and only five isolates represented by BBB1 caused black spots around the inoculation sites after 10 days post-incubation (dpi).Moreover, the symptoms Forests 2022, 13, 1616 6 of 12 were similar to the disease symptoms observed in the wild.The spots turned black both outside and inside the bamboo culm pieces (Figure 2A), and no symptoms developed on the control (Figure 2B).The same fungus was recovered from the experimental inoculation.The isolates were deposited in the Forest Protection Institute of Fujian Agriculture and Forestry University, Fuzhou City, Fujian Province, China.
spots around the inoculation sites after 10 days post-incubation (dpi).Moreover, the symptoms were similar to the disease symptoms observed in the wild.The spots black both outside and inside the bamboo culm pieces (Figure 2A), and no symptoms de veloped on the control (Figure 2B).The same fungus was recovered from the experimenta inoculation.The isolates were deposited in the Forest Protection Institute of Fujian Agri culture and Forestry University, Fuzhou City, Fujian Province, China.
In the field, isolate BBB1 could infect the one-year-old Moso bamboo culms and cause culm necrosis after 14 dpi (Figure 2E).The spots on the five-year-old culms extended to a rhomboid shape of approximately 2.8 cm (length) × 1.0 cm (width) in size after fou months post-inoculation (Figure 2D).The same fungus was reisolated from the transi tional area between the diseased and healthy tissues based on morphological and molec ular characterizations.

Morphological Characteristics of Pathogenic Fungus
On PDA, colonies of BBB1 grew and reached 7.36-7.80(average of 7.56) cm in diam eter after four days of incubation (1.89 cm per day).The colonies were dirty white with aerial mycelia (Figure 3A).On MEA, colonies of BBB1 were white with aerial mycelia (Fig ure 3B).Both hyphae were smooth, hyaline, branched, and septate on PDA and MEA (Fig ure 3A,B).The conidiophores were reduced to conidiogenous cells and measured 7.0-12.6μm × 2.7-4.6 μm (length × width; average of 9.4 × 3.7 μm; n = 50), were pale brown to dark smooth, and ampulliform (Figure 3C).The conidia were 5.0-9.3 × 3.1-7.3μm (average o 6.4 × 5.4 μm; n = 100), single-celled, brown to dark, smooth, anomalous in shape (globose In the field, isolate BBB1 could infect the one-year-old Moso bamboo culms and cause culm necrosis after 14 dpi (Figure 2E).The spots on the five-year-old culms extended to a rhomboid shape of approximately 2.8 cm (length) × 1.0 cm (width) in size after four months post-inoculation (Figure 2D).The same fungus was reisolated from the transitional area between the diseased and healthy tissues based on morphological and molecular characterizations.

Figure 4.
The phylogenetic tree of the isolate BBB1 with allied taxa analyzed using concatenated sequences of ITS, TEF1-α, and TUB2 with the maximum likelihood method.Nigrospora bambusa was used as the outgroup.

Sensitivity of A. arundinis BBB1 to Fungicides
The sensitivity of A. arundinis isolate BBB1 from Moso bamboo against six commonly used fungicides was determined with mycelial growth assays in vitro.The six tested fungicides were difenoconazole, hymexazol, iprodione, mancozeb, prochloraz, and propiconazole, and their EC50 values were 0.195, 170.520, 5.627, 267.514, 0.019, and 4.324 mg/L, respectively (Table 2).Prochloraz exhibited the most potent inhibition against the mycelial growth of the pathogen isolate BBB1, with an EC50 value of 0.019 mg/L.Another effective fungicide was difenoconazole, with an EC50 value of 0.195 mg/L.Meanwhile, hymexazol and mancozeb needed high concentrations to inhibit the growth of the pathogen isolate BBB1.

Sensitivity of A. arundinis BBB1 to Fungicides
The sensitivity of A. arundinis isolate BBB1 from Moso bamboo against six commonly used fungicides was determined with mycelial growth assays in vitro.The six tested fungicides were difenoconazole, hymexazol, iprodione, mancozeb, prochloraz, and propiconazole, and their EC 50 values were 0.195, 170.520, 5.627, 267.514, 0.019, and 4.324 mg/L, respectively (Table 2).Prochloraz exhibited the most potent inhibition against the mycelial growth of the pathogen isolate BBB1, with an EC 50 value of 0.019 mg/L.Another effective fungicide was difenoconazole, with an EC 50 value of 0.195 mg/L.Meanwhile, hymexazol and mancozeb needed high concentrations to inhibit the growth of the pathogen isolate BBB1.

Discussion
Arthrinium is a globally distributed genus inhabiting a wide range of hosts as saprophytes and endophytes on plants.The genus also includes phytopathogenic species such as A. arundinis, which causes kernel blight of barley in the USA, and A. marii, which causes olive tree dieback in Italy [27,35].Recently, some new species and/or new bamboo hosts have been reported [5,17,18,20,21].In this study, A. arundinis was isolated from the culms of Moso bamboo (P.edulis) and confirmed as the pathogen of culm rhomboid rot by fulfilling Koch's postulates. A. arundinis has been reported to cause the brown culm streaks on P. praecox, which the streaks expanding to a maximum of 0.3 cm [17], but we found that A. arundinis infected Moso bamboo causing lesions of up to 15 cm in length and invading the inside of the culms.The significant differences in the disease symptoms may be due to the different hosts or differences in the pathogenicity of the pathogens.In addition, similar behaviors were not only reported for Arthrinium on Moso bamboo but Fusarium oxysporum has been reported to cause culm rhomboid rot in Moso bamboo in Jiangxi Province, China [9].Unfortunately, none of the Fusarium strains (Figure S1B-E) we isolated exhibited pathogenicity.To the best of our knowledge, this is the first report of A. arundinis causing culm rhomboid rot of Moso bamboo in China or worldwide.
According to our survey, the disease seriously occurred on aged (five-year-old and above) Moso bamboo in the wild; in the artificial inoculation test, A. arundinis could cause disease in new (one-year-old) and aged (five-year-old) Moso bamboo culm, and wounds were required for disease occurrence both in the laboratory and under field conditions (inoculation without wounds could not induce rot; data not shown).Furthermore, we found that swarms of scale insects inhabit aged Moso bamboo culms (Figure S2A,B); do the biting spots caused by scale insects contribute to A. arundinis infection?It has long been assumed that F. verticillioides is an opportunistic fungus, where it takes advantage of the openings left by Diatraea saccharalis caterpillar attack to infect sugarcane.One report presented by Franco et al. in 2021 showed that volatile compounds from F. verticillioides attract D. saccharalis caterpillars.Once they become adults, the fungus is transmitted vertically to their offspring, which continues the cycle by inoculating the fungus in healthy sugarcane [36].The interaction among bamboo, scale insects, and A. arundinis needs further investigation.Based on this, we infer that the management of scale insects on aged Moso bamboo may be important for controlling culm rhomboid rot disease.
Initially, we supposed that the pink fruitbody fungi around the diseased spots were the pathogen.However, the pure culture could not be obtained, and the ascospores collected from the fruitbody could not induce the rot in Moso bamboo culms.The nonpathogenic pink fruitbody fungi were found around the rot spots on Moso bamboo in Fujian Province and identified as Podonectria based on its morphological characteristics and habitats (Figure S2C-E,H,I).It was difficult to obtain a pure colony of Podonectria, a fungal genus, under cultural conditions and which used to be entomopathogenic fungi of armored scale insects [37][38][39].Podonectria has also been reported on P. heteroclada from Sichuan Province, China, considered to be a factor that expands necrotic spots [6,40].We found that Podonectria not only colonized around the necrotic spots, but it was also widely distributed via armored scale insects (Figure S2A,B).The function of Podonectria in this disease is unknown, and the relationship and interactions among A. arundinis, Podonectria, and armored scale insects should be investigated further.During the field survey, we found that the surface of the Moso bamboo culms was easily covered with a complex of dirt, moss, and sooty mold under a highly humid environment, which resulted in the diseased spots hardly being observed, but the pink ascomata of Podonectria around the necrosis were obvious (Figure S2F,G).It is certain that Podonectria acted as a marker for culm rhomboid rot spots in the early stage.
Several species of Arthrinium have been documented as plant pathogens, although information on pathogen biology and disease epidemiology and management is generally lacking [35].The sensitivity of A. arundinis BBB1 against six commonly used fungicides (difenoconazole, prochloraz, propiconazole, iprodione, hymexazol, and mancozeb) was evaluated.Prochloraz exhibited the highest toxicity to fungal growth.Prochloraz is a DMI (demethylation inhibitor)-imidazole fungicide with a biochemical mode of action based on the inhibition of the ergosterol biosynthesis pathway and has been used as a protectant or eradicate [41,42].Another effective fungicide is difenoconazole, which is a triazole fungicide.Considering the production of resistance to prochloraz, we recommend that prochloraz and difenoconazole be applied alternately for disease management.
The field survey found that disease occurrence is very complex.Although we determined the pathogen and provided some reasonable fungicides based on a sensitivity test of the pathogen indoors, the plant-pathogen-scale insect interaction also needs to be paid more attention in the field control.

Conclusions
In conclusion, we confirmed the causal agent of culm rhomboid rot disease in Moso bamboo and identified the pathogen as A. arundinis according to its morphology and phylogenetic analysis.This is the first report of A. arundinis as the pathogen of Moso bamboo culm rhomboid rot.Wounds on the Moso bamboo culm surface were required for disease occurrence. A. arundinis was more sensitive to the fungicides prochloraz (Ec 50 value of 0.019 mg/L) and difenoconazole (EC 50 value of 0.195 mg/L).This study could provide a scientific basis for the prevention and control of Moso bamboo culm rhomboid rot disease in Fujian Province, China.

Figure 1 .
Figure 1.Symptoms of moso bamboo (Phyllostachys edulis) culm rhomboid rot disease in bamboo forests.Scale bar: 5 cm.(A) Black spots and rhomboid shape symptoms on the outer surface; (B) Symptoms covered with a complex of dirt, moss, and sooty mold; (C) Macroscopic pink fungal ascomata appeared, surrounding the spots; (D) Rhomboid shape symptoms with sooty mold and pink fungal ascomata; (E,F) Necrosis occur on the inner cavity of the culm.

Figure 2 .
Figure 2. Pathogenicity test.(A) Symptoms on Moso bamboo culm inoculated with the isolate BBB1 after 10 days; (B) Control Moso bamboo culm inoculated with PDA plug; (C) Symptoms on 5-year old Moso bamboo inoculated with the isolate BBB1 after 4 months, the upper three wounds inocu lated with the isolate BBB1, and the lower one with PDA plug; (D) Details under the diseased tis sues; (E) Symptoms on 1-year-old Moso bamboo inoculated with the isolate BBB1 after 14 days; (F 1-year-old control Moso bamboo inoculated with PDA plug.

Figure 2 .
Figure 2. Pathogenicity test.(A) Symptoms on Moso bamboo culm inoculated with the isolate BBB1 after 10 days; (B) Control Moso bamboo culm inoculated with PDA plug; (C) Symptoms on 5-year-old Moso bamboo inoculated with the isolate BBB1 after 4 months, the upper three wounds inoculated with the isolate BBB1, and the lower one with PDA plug; (D) Details under the diseased tissues; (E) Symptoms on 1-year-old Moso bamboo inoculated with the isolate BBB1 after 14 days; (F) 1-year-old control Moso bamboo inoculated with PDA plug.

Figure 4 .
Figure 4.The phylogenetic tree of the isolate BBB1 with allied taxa analyzed using concatenated sequences of ITS, TEF1-α, and TUB2 with the maximum likelihood method.Nigrospora bambusa was used as the outgroup.
195019.52 E, 27•11 58.12 N), Jianyang District and Dazhulan Bamboo Forest (117 • 39 24.11 E, 27 • 42 1.37 N), Wuyishan County, Nanping City, Fujian Province, China.The symptoms were photographed using a Canon EOS M6 digital camera, and the size of the spots (50 spots) was measured.Specimens of culm rhomboid rot in Moso bamboo were collected from Huajiashan Moso Bamboo Farm in April 2021 (average precipitation of 211 mm, average temperature of 20 • C, and altitude of 840 m) and kept in the Forest Protection Institute of Fujian Agriculture and Forestry University.

Table 1 .
Details of Arthrinium.spp.used in this study.

Table 2 .
Toxicity of six fungicides against the mycelial growth of Arthrinium arundinis isolate BBB1.

Table 2 .
Toxicity of six fungicides against the mycelial growth of Arthrinium arundinis isolate BBB1.