Role of Species and Planting Conﬁguration on Transpiration and Microclimate for Urban Trees

: Research Highlights: To demonstrate the e ﬀ ectiveness of conﬁguration modes and tree types in regulating local microclimate. Background and Objectives: Urban trees play an essential role in reducing the city’s heat load. However, the inﬂuence of urban trees with di ﬀ erent conﬁgurations on the urban thermal environment has not received enough attention. Herein we show how spatial arrangement and foliage longevity, deciduous versus evergreen, a ﬀ ect transpiration and the urban microclimate. Materials and Methods: We analyzed the di ﬀ erences between physiological parameters (transpiration rate, stomatal conductance) and meteorological parameters (air temperature, relative humidity, vapor pressure deﬁcit) of 10 di ﬀ erent species of urban trees (ﬁve evergreen and ﬁve deciduous tree species), each of which had been planted in three conﬁguration modes in a park and the campus green space in Xi’an. By manipulating physiological parameters, crown morphology, and plant conﬁgurations, we explored how local urban microclimate could be altered. Results: (1) Microclimate regulation capacity: group planting (GP) > linear planting (LP) > individual planting (IP). (2) Deciduous trees (DT) regulated microclimate better than evergreen trees (ET). Signiﬁcant di ﬀ erences between all planting conﬁgurations during 8 to 16 h were noted for evergreen trees whereas for deciduous trees, all measurement times were signiﬁcantly di ﬀ erent. (3) Transpiration characteristics: GP > LP > IP. The transpiration rate (E) and stomatal conductance (G s ) of GP were the highest. Total daily transpiration was ranked as group planting of deciduous (DGP) > linear planting of deciduous (DLP) > group planting of evergreen (EGP) > linear planting of evergreen (ELP) > isolated planting of deciduous (DIP) > isolated planting of evergreen (EIP). (4) The microclimate e ﬀ ects of di ﬀ erent tree species and conﬁguration modes were positively correlated with E, G s , and three dimensional green quantity (3DGQ), but weakly correlated with vapor pressure deﬁcit (Vpd L ). (5) A microclimate regulation capability model of urban trees was developed. E, G s , and 3DGQ could explain 93% variation of cooling e ﬀ ect, while E, G s , Vpd L , and 3DGQ could explain 85% variation of humidifying e ﬀ ect. Conclusions: This study demonstrated that the urban heat island could be mitigated by selecting deciduous broadleaf tree species and planting them in groups.


Introduction
Because of the ability of trees and their foliage to reflect short-wave radiation, to re-radiate long-wave radiation, to shade, and to cool via transpiration, trees in an urban environment have the potential to alter the microclimate of that environment [1][2][3][4][5]. The urban heat island is a well-known

Experiment Design
Field trials were carried out on the campus of Northwest Agriculture and Forestry University and the Yangling arboretum park covering an area of about 6 Ha, 1.4 km away from each other ( Figure 1). The experimental objects were 10 different species of common urban trees planted in three different configuration modes (Individual planting, linear planting, group planting), five species were deciduous broadleaf and five were evergreen broadleaf, with similar tree ages, no diseases and insect pests, and good growth vitality ( Table 1). The test plots were all covered with grass. There were no trees taller than 1 m within the 2 m distance from the crown projected area around the individual planting samples. The sample plots with the same tree species, fixed plant row spacing, five tree species, non-overlapping crown, and single row planting arrangement were selected. Test samples with an enclosing area of 15 m × 15 m of the same tree species were chosen for group planting, all tree species of group planting were planted irregularly and the crowns did not overlap with each other, allowing users to enter freely ( Figure 2). There were no tall trees above the selected sites, and all of them were far away from water, which excludes the influence of water on the microclimate effect of the measuring points. Meanwhile, we selected 30 hard open fields as control sites, which were no vegetation coverage and located within our study area and no more than 50 m away from each test sample.

Experiment Design
Field trials were carried out on the campus of Northwest Agriculture and Forestry University and the Yangling arboretum park covering an area of about 6 Ha, 1.4 km away from each other ( Figure 1). The experimental objects were 10 different species of common urban trees planted in three different configuration modes (Individual planting, linear planting, group planting), five species were deciduous broadleaf and five were evergreen broadleaf, with similar tree ages, no diseases and insect pests, and good growth vitality ( Table 1). The test plots were all covered with grass. There were no trees taller than 1 m within the 2 m distance from the crown projected area around the individual planting samples. The sample plots with the same tree species, fixed plant row spacing, five tree species, non-overlapping crown, and single row planting arrangement were selected. Test samples with an enclosing area of 15 m × 15 m of the same tree species were chosen for group planting, all tree species of group planting were planted irregularly and the crowns did not overlap with each other, allowing users to enter freely ( Figure 2). There were no tall trees above the selected sites, and all of them were far away from water, which excludes the influence of water on the microclimate effect of the measuring points. Meanwhile, we selected 30 hard open fields as control sites, which were no vegetation coverage and located within our study area and no more than 50 m away from each test sample.

Experiment Design
Field trials were carried out on the campus of Northwest Agriculture and Forestry University and the Yangling arboretum park covering an area of about 6 Ha, 1.4 km away from each other ( Figure 1). The experimental objects were 10 different species of common urban trees planted in three different configuration modes (Individual planting, linear planting, group planting), five species were deciduous broadleaf and five were evergreen broadleaf, with similar tree ages, no diseases and insect pests, and good growth vitality ( Table 1). The test plots were all covered with grass. There were no trees taller than 1 m within the 2 m distance from the crown projected area around the individual planting samples. The sample plots with the same tree species, fixed plant row spacing, five tree species, non-overlapping crown, and single row planting arrangement were selected. Test samples with an enclosing area of 15 m × 15 m of the same tree species were chosen for group planting, all tree species of group planting were planted irregularly and the crowns did not overlap with each other, allowing users to enter freely ( Figure 2). There were no tall trees above the selected sites, and all of them were far away from water, which excludes the influence of water on the microclimate effect of the measuring points. Meanwhile, we selected 30 hard open fields as control sites, which were no vegetation coverage and located within our study area and no more than 50 m away from each test sample.

Microclimate Measurements
Measurements were performed on July 12th, 13th, and 20th, 2019. Since wind speed has a significant influence on the cooling effect of trees [31], so sunny days, less cloud cover, and no wind (wind speed ≤ 2 m/s) were selected. Ph-II hand-held weather station (Wuhan Xinpuhui Technology Co., Ltd., Wuhan, China) was used to determine the air temperature (T a0 ) and relative humidity (RH 0 ) in the 30 test sample sites. Trials took place from 8 to 18 h each day, and records were done every 2 h. In order to calculate the intensity of cooling and humidification of the sample plot, air temperature (T a ) and relative humidity (RH) at the center of the control sites were measured simultaneously ( Table 2). The maximum temperature during the experiment was about 37.5 • C and the vapor pressure deficit (VPD) was about 2.5 Kpa. All instruments were installed at 1.5 m above the ground, which is the average height of human respiration. Each sample plot was continuously monitored for at least 10 min one time and the relative statistics were recorded every other minute.

Measuring Points
The eight measuring points of individual planting plots were arranged in a "meter" shape with a radius of 1 m from the central point of the canopy. The measuring points of linear planting plots were set under each tree in the directions of east, south, west, and north, all of which were 1 m away from the central point of canopy, and set a measuring point between every two trees. There were altogether 17 measuring points in each group planting plot, one of the points were arranged in the center and the rest 16 points which were averagely distributed in four directions of east, south, west, and north were arranged every 2 m along the central axis of the test sample ( Figure 3). The average value of all Forests 2020, 11, 825 5 of 18 measuring points is the air temperature and relative humidity of the sample plot. The average daily cooling and humidifying effects of each measurement point were calculated as follows: (1) T a and RH are the air temperature ( • C) and relative humidity (%) of the control point, respectively.

Transpiration Measurements
Physiological sampling was carried out simultaneously with measures of microclimatology. The test was performed on sunny days and vapor pressure deficit (VPD) was about 2.5 Kpa. Li-6400xt system (LI-COR, Lincoln, NE, USA) with the 2 × 3 cm chamber and red/blue light source was used to measure leaf level transpiration every 2 h from 8 to 18 h. The third, fourth, and fifth leaves from the tip leaf along the branch axis from the mid-canopy of each species were each measured three times. For trees planted in a linear configuration, the first, middle, and last trees were measured. Whereas for the group configuration, four trees within the sample plot were randomly selected and measured. Transpiration rate (E, mmolH2O/m 2 s) and stomatal conductance (Gs, molH2O/m 2 s) were measured; simultaneously, leaf temperature (T) and relative humidity (RH) were measured and vapor pressure deficit was calculated as shown in Equation (3) [32]: T is the blade temperature (°C), RH is the relative air humidity (%), a, b, and C are constants. a = 0.611, b = 17.502, C = 240.97. Daily transpiration of plant unit leaf area (EL):

Transpiration Measurements
Physiological sampling was carried out simultaneously with measures of microclimatology. The test was performed on sunny days and vapor pressure deficit (VPD) was about 2.5 Kpa. Li-6400xt system (LI-COR, Lincoln, NE, USA) with the 2 × 3 cm chamber and red/blue light source was used to measure leaf level transpiration every 2 h from 8 to 18 h. The third, fourth, and fifth leaves from the tip leaf along the branch axis from the mid-canopy of each species were each measured three times. For trees planted in a linear configuration, the first, middle, and last trees were measured. Whereas for the group configuration, four trees within the sample plot were randomly selected and measured. Transpiration rate (E, mmolH 2 O/m 2 s) and stomatal conductance (G s , molH 2 O/m 2 s) were measured; simultaneously, leaf temperature (T) and relative humidity (RH) were measured and vapor pressure deficit was calculated as shown in Equation (3) [32]: T is the blade temperature ( • C), RH is the relative air humidity (%), a, b, and C are constants. a = 0.611, b = 17.502, C = 240.97. Daily transpiration of plant unit leaf area (E L ): e i and e i+1 are respectively the instantaneous transpiration rates of the initial measurement point and the next measurement point, and the unit is mmol/m 2 s. t i and t i+1 are respectively the instantaneous time of the initial measurement point and the next measurement point, and the unit is h. N is the number of tests, 3600 s per hour, 1 mol = 1000 mmol.

Statistical Analyses
SPSS22.0 (IBM ® SPSS ® Statistics, Armonk, NY, USA) software was used to conduct a single sample K-S test for all independent variables and dependent variables to verify whether the data met the normal distribution. In order to compare the differences of average daily air temperature and average daily relative humidity between each measuring point and the control point, paired-samples t-test was used. One-way analysis of variance (ANOVA) was used to compare the average daily cooling effect and average daily humidifying effect of the three configuration modes of urban trees, and then Duncan test was conducted. Two-way analysis of variance (ANOVA) was used to compare the differences in the microclimate regulation capabilities of the three configurations of evergreen and deciduous tree species. Correlation analysis was employed to explore the relationship between the microclimate effect of urban trees with three different configuration modes and E, G s , Vpd L , and 3DGQ, and the studied parameters for the microclimate effect of urban trees were determined through stepwise regression. To explore the extent to which each parameter explains the microclimate effect, Amos 24.0 software (IBM, Armonk, NY, USA) was utilized to create the structural equation model (SEM). In all the comparisons, p < 0.05 was considered statistically significant. All diagrams were drawn using Simaplot14.0 (Systat Software, Inc., San Jose, CA, USA) and Origin2019 (Origin Lab Corp, Northampton, MA, USA). Figure 4 depicts the diurnal variations of air temperature and relative humidity of the three configuration modes and the control sites. Air temperature of the three configuration modes and the control sites continuously rose from 8 to 14 h, reached a single peak at 14 h and then decreased between 14 and 18 h. Humidity declined and recovered in an inverted pattern. During the test, the diurnal variations of air temperature and relative humidity in the three configuration modes and the control sites all showed the shape of "U". In general, although the air temperature and relative humidity variation trends of individual planting (IP), linear planting (LP), and group planting (GP) were similar, air temperature and relative humidity were not statistically significant, but were both numerically greater for the IP versus LP versus for the GP configurations. ei and ei+1 are respectively the instantaneous transpiration rates of the initial measurement point and the next measurement point, and the unit is mmol/m 2 s. ti and ti+1 are respectively the instantaneous time of the initial measurement point and the next measurement point, and the unit is h. N is the number of tests, 3600 s per hour, 1 mol = 1000 mmol.

Statistical Analyses
SPSS22.0 (IBM ® SPSS ® Statistics, Armonk, NY, USA) software was used to conduct a single sample K-S test for all independent variables and dependent variables to verify whether the data met the normal distribution. In order to compare the differences of average daily air temperature and average daily relative humidity between each measuring point and the control point, paired-samples t-test was used. One-way analysis of variance (ANOVA) was used to compare the average daily cooling effect and average daily humidifying effect of the three configuration modes of urban trees, and then Duncan test was conducted. Two-way analysis of variance (ANOVA) was used to compare the differences in the microclimate regulation capabilities of the three configurations of evergreen and deciduous tree species. Correlation analysis was employed to explore the relationship between the microclimate effect of urban trees with three different configuration modes and E, Gs, VpdL, and 3DGQ, and the studied parameters for the microclimate effect of urban trees were determined through stepwise regression. To explore the extent to which each parameter explains the microclimate effect, Amos 24.0 software (IBM, Armonk, NY, USA) was utilized to create the structural equation model (SEM). In all the comparisons, p < 0.05 was considered statistically significant. All diagrams were drawn using Simaplot14.0 (Systat Software, Inc., San Jose, CA, USA) and Origin2019 (Origin Lab Corp, Northampton, MA, USA). Figure 4 depicts the diurnal variations of air temperature and relative humidity of the three configuration modes and the control sites. Air temperature of the three configuration modes and the control sites continuously rose from 8 to 14 h, reached a single peak at 14 h and then decreased between 14 and 18 h. Humidity declined and recovered in an inverted pattern. During the test, the diurnal variations of air temperature and relative humidity in the three configuration modes and the control sites all showed the shape of "U". In general, although the air temperature and relative humidity variation trends of individual planting (IP), linear planting (LP), and group planting (GP) were similar, air temperature and relative humidity were not statistically significant, but were both numerically greater for the IP versus LP versus for the GP configurations.

Microclimate Regulation Capabilities of Urban Trees in Different Types and Different Configuration Modes
We undertook a statistical analysis of the microclimate regulation capabilities of the three configuration modes (Tables S1 and S2). The results demonstrated that the average cooling effects of IP, LP, and GP were 1.02 ± 0.78 • C, 1.37 ± 0.96 • C, and 2.05 ± 0.97 • C, respectively (Table S1). The average humidifying effects of IP, LP, and GP were 2.10% ± 1.8%, 2.75% ± 2.08%, and 3.45% ± 2.15%, respectively (Table S2). In order to compare the microclimate regulation capabilities of the three configuration modes, post hoc tests were performed, and the results showed that there were significant differences in cooling and humidifying effects between IP, LP, and GP (p < 0.01), all of which indicate that GP had the most robust microclimate regulation capability, LP came second, and IP had the weakest microclimate regulation capability.
The daily variation trend of the air temperature of individual planting of evergreen (EIP), linear planting of evergreen (ELP), group planting of evergreen (EGP), individual planting of deciduous (DIP), linear planting of deciduous (DLP), group planting of deciduous (DGP), and the control sites all showed a daily trend of first rising and then falling, the maximum temperature for the day was reached at 14 h ( Figure 5a). The air temperatures at all measuring points were always lower than those at the control sites. The cooling effects of EGP and DGP were significantly different from 8 to 14 h (p < 0.05), likewise between the cooling effects of ELP and DLP at 12 h and 14 h (p < 0.05), and the cooling effects of EIP and DIP were significantly different at 12 h and 18 h (p < 0.05). The cooling effects of DGP and DLP were not significantly different at 8 h (p > 0.05), but significantly different (p < 0.05) at other time points. The cooling effect of EGP, ELP, and EIP were significantly different from 8 to 16 h (p < 0.05). Figure 5b shows that the daily variation trend of relative humidity in the three configuration modes and the control sites were similar, the relative humidity at all measuring points were always lower than that at the control sites. Moreover, the actual vapor content of the air stayed the same and the humidity changed because the temperature changed. As for the humidifying effects at each measuring point, DGP and DLP were not significantly different at 10 h and 14 h (p > 0.05), likewise between ELP and DLP from 14 to 18 h (p > 0.05). There was a significant difference between EIP and DIP from 8 to 12 h (p < 0.05), and the humidifying effects of DGP, DLP, and DIP were significantly different at each measurement point (p < 0.05).

Microclimate Regulation Capabilities of Urban Trees in Different Types and Different Configuration Modes
We undertook a statistical analysis of the microclimate regulation capabilities of the three configuration modes (Tables S1 and S2). The results demonstrated that the average cooling effects of IP, LP, and GP were 1.02 ± 0.78 °C, 1.37 ± 0.96 °C, and 2.05 ± 0.97 °C, respectively (Table S1). The average humidifying effects of IP, LP, and GP were 2.10% ± 1.8%, 2.75% ± 2.08%, and 3.45% ± 2.15%, respectively (Table S2). In order to compare the microclimate regulation capabilities of the three configuration modes, post hoc tests were performed, and the results showed that there were significant differences in cooling and humidifying effects between IP, LP, and GP (p < 0.01), all of which indicate that GP had the most robust microclimate regulation capability, LP came second, and IP had the weakest microclimate regulation capability.
The daily variation trend of the air temperature of individual planting of evergreen (EIP), linear planting of evergreen (ELP), group planting of evergreen (EGP), individual planting of deciduous (DIP), linear planting of deciduous (DLP), group planting of deciduous (DGP), and the control sites all showed a daily trend of first rising and then falling, the maximum temperature for the day was reached at 14 h ( Figure 5a). The air temperatures at all measuring points were always lower than those at the control sites. The cooling effects of EGP and DGP were significantly different from 8 to 14 h (p < 0.05), likewise between the cooling effects of ELP and DLP at 12 h and 14 h (p < 0.05), and the cooling effects of EIP and DIP were significantly different at 12 h and 18 h (p < 0.05). The cooling effects of DGP and DLP were not significantly different at 8 h (p > 0.05), but significantly different (p < 0.05) at other time points. The cooling effect of EGP, ELP, and EIP were significantly different from 8 to 16 h (p < 0.05). Figure 5b shows that the daily variation trend of relative humidity in the three configuration modes and the control sites were similar, the relative humidity at all measuring points were always lower than that at the control sites. Moreover, the actual vapor content of the air stayed the same and the humidity changed because the temperature changed. As for the humidifying effects at each measuring point, DGP and DLP were not significantly different at 10 h and 14 h (p > 0.05), likewise between ELP and DLP from 14 to 18 h (p > 0.05). There was a significant difference between EIP and DIP from 8 to 12 h (p < 0.05), and the humidifying effects of DGP, DLP, and DIP were significantly different at each measurement point (p < 0.05).

Transpiration Characteristics of Urban Trees with Different Tree Types and Configuration Modes
It can be observed in Figure 6a that the transpiration rates of IP, LP, and GP of ET all showed the same diurnal variation trend, reaching the peak at 12 h. The daily variation trend of Gs of IP, LP, and GP of ET were roughly the same, and the average stomatal conductance ranking at each time point was ranked as GP > LP > IP. With the increase of air temperature in the samples, VpdL also increased and reached a peak at 14 h. With the decrease of air temperature and the increase of relative humidity, VpdL declined, and the value of VpdL at 18 h was more significant than that at 8 h, indicating that the air temperature in the samples in the afternoon is higher than that in the early morning. Figure 6b indicates that E and Gs of DT with the three configuration modes showed the same diurnal variation trend. The peak value of VpdL in deciduous tree species was at 14 h, and the value at this time was 2.4 times of 8 h. E and Gs of the measured time point were the highest in GP, followed by LP and the lowest in IP. This indicates that there are differences in transpiration between different configuration modes, and the tree species of GP configuration have the most robust transpiration characteristics.
A detailed examination and comparison of the distribution of E and Gs values for the three different planting configurations, followed by a comparison of evergreen versus deciduous species in the three different planting configurations (data not shown, see Figures S1 and S2) resulted in statistically significant differences. For E, critical for cooling and humidifying the air, and the mean value was ranked as GP > LP > IP. In addition, E in deciduous species was statistically greater than E in evergreen species. The average daily transpiration (EL) of ET and DT in three different configuration modes was significantly different (F = 9.107, p < 0.001). The value of DGP was the highest, followed by DLP, EGP, ELP, DIP, and the value of EIP was the lowest (Figure 7). Among them, the total transpiration value of all experimental species from 12 to 14 h was the highest, and EL was ranked as GP > LP > IP.

Transpiration Characteristics of Urban Trees with Different Tree Types and Configuration Modes
It can be observed in Figure 6a that the transpiration rates of IP, LP, and GP of ET all showed the same diurnal variation trend, reaching the peak at 12 h. The daily variation trend of G s of IP, LP, and GP of ET were roughly the same, and the average stomatal conductance ranking at each time point was ranked as GP > LP > IP. With the increase of air temperature in the samples, Vpd L also increased and reached a peak at 14 h. With the decrease of air temperature and the increase of relative humidity, Vpd L declined, and the value of Vpd L at 18 h was more significant than that at 8 h, indicating that the air temperature in the samples in the afternoon is higher than that in the early morning. Figure 6b indicates that E and G s of DT with the three configuration modes showed the same diurnal variation trend. The peak value of Vpd L in deciduous tree species was at 14 h, and the value at this time was 2.4 times of 8 h. E and G s of the measured time point were the highest in GP, followed by LP and the lowest in IP. This indicates that there are differences in transpiration between different configuration modes, and the tree species of GP configuration have the most robust transpiration characteristics.
A detailed examination and comparison of the distribution of E and G s values for the three different planting configurations, followed by a comparison of evergreen versus deciduous species in the three different planting configurations (data not shown, see Figures S1 and S2) resulted in statistically significant differences. For E, critical for cooling and humidifying the air, and the mean value was ranked as GP > LP > IP. In addition, E in deciduous species was statistically greater than E in evergreen species. The average daily transpiration (E L ) of ET and DT in three different configuration modes was significantly different (F = 9.107, p < 0.001). The value of DGP was the highest, followed by DLP, EGP, ELP, DIP, and the value of EIP was the lowest (Figure 7). Among them, the total transpiration value of all experimental species from 12 to 14 h was the highest, and E L was ranked as GP > LP > IP.

Correlation between Microclimate Regulation Ability and Influencing Factors of Urban Trees with Different Configuration Modes
In order to explore the relative contributions of meteorological parameters, transpiration characteristics and 3DGQ to the microclimate regulation ability of urban trees with different configuration modes, Spearman correlation analysis was conducted on the transpiration characteristics of the three configuration modes and their cooling and humidifying effects ( Table 3). The cooling and humidifying effects of the three configuration modes all had strong positive correlation with E, Gs, and 3DGQ (p < 0.01), while there was a weak positive correlation with VpdL. However, the cooling and humidifying effects of the three configuration modes were independent of air temperature and relative humidity, and there was a strong positive correlation between the cooling and humidifying effects (p < 0.01).

Correlation between Microclimate Regulation Ability and Influencing Factors of Urban Trees with Different Configuration Modes
In order to explore the relative contributions of meteorological parameters, transpiration characteristics and 3DGQ to the microclimate regulation ability of urban trees with different configuration modes, Spearman correlation analysis was conducted on the transpiration characteristics of the three configuration modes and their cooling and humidifying effects ( Table 3). The cooling and humidifying effects of the three configuration modes all had strong positive correlation with E, Gs, and 3DGQ (p < 0.01), while there was a weak positive correlation with VpdL. However, the cooling and humidifying effects of the three configuration modes were independent of air temperature and relative humidity, and there was a strong positive correlation between the cooling and humidifying effects (p < 0.01).

Correlation between Microclimate Regulation Ability and Influencing Factors of Urban Trees with Different Configuration Modes
In order to explore the relative contributions of meteorological parameters, transpiration characteristics and 3DGQ to the microclimate regulation ability of urban trees with different configuration modes, Spearman correlation analysis was conducted on the transpiration characteristics of the three configuration modes and their cooling and humidifying effects ( Table 3). The cooling and humidifying effects of the three configuration modes all had strong positive correlation with E, G s , and 3DGQ (p < 0.01), while there was a weak positive correlation with Vpd L . However, the cooling and humidifying effects of the three configuration modes were independent of air temperature and relative humidity, and there was a strong positive correlation between the cooling and humidifying effects (p < 0.01). There were significant differences between slope and intercept of response of 3DGQ of IP, LP, and GP to the cooling and humidifying effect, which also indicated that the cooling and humidifying effect increases with the increase of 3DGQ (Figure 8a,b). 3DGQ is also an important parameter to measure the microclimate regulation ability of urban trees. When 3DGQ < 5 m 3 , the microclimate effects of trees were very weak. Besides, the humidifying effect of GP and 3DGQ's linear model had the highest fitting coefficient among all models.
There were significant differences between slope and intercept of response of 3DGQ of IP, LP, and GP to the cooling and humidifying effect, which also indicated that the cooling and humidifying effect increases with the increase of 3DGQ (Figure 8a, b). 3DGQ is also an important parameter to measure the microclimate regulation ability of urban trees. When 3DGQ < 5 m 3 , the microclimate effects of trees were very weak. Besides, the humidifying effect of GP and 3DGQ's linear model had the highest fitting coefficient among all models.
The transpiration rates (E) of IP, LP, and GP are significantly different from each other in the slope and intercept of the response to cooling and humidifying effects ( Figure S3a, d). This indicates that the cooling and humidifying effects of urban trees in the three configuration modes are enhanced with the increase of E.
The stomatal conductance (Gs) of IP, LP, and GP showed significant differences in the slope and intercept of the response to cooling and humidifying effects, and the microclimate effects of the three configuration modes of urban tree species increased with the increase of Gs ( Figure S3b, e). It is implied that E and Gs are essential parameters to be considered when measuring the microclimate regulation capacity of urban trees.
It is observed from Table 3 that the correlation coefficient between the cooling effect of IP and its VpdL was 0.330, which was a weak correlation (p < 0.05) so that it was impossible to establish a functional model (R 2 = 0.078, p = 0.031), and the correlation coefficient between humidifying effect and VpdL was 0.352, also proving impossible to establish a functional model (R 2 = 0.093, p = 0.015). However, the vapor pressure deficit (VpdL) of LP and GP had built a linear relationship model with the cooling effect and humidifying effect respectively ( Figure S3c, f). In general, the influence of configuration mode on the correlation between microclimate effects of urban tree species and VpdL was less than that between the above transpiration characteristic and microclimate effects.  The transpiration rates (E) of IP, LP, and GP are significantly different from each other in the slope and intercept of the response to cooling and humidifying effects ( Figure S3a,d). This indicates that the cooling and humidifying effects of urban trees in the three configuration modes are enhanced with the increase of E.
The stomatal conductance (G s ) of IP, LP, and GP showed significant differences in the slope and intercept of the response to cooling and humidifying effects, and the microclimate effects of the three configuration modes of urban tree species increased with the increase of G s (Figure S3b,e). It is implied that E and G s are essential parameters to be considered when measuring the microclimate regulation capacity of urban trees.
It is observed from Table 3 that the correlation coefficient between the cooling effect of IP and its Vpd L was 0.330, which was a weak correlation (p < 0.05) so that it was impossible to establish a functional model (R 2 = 0.078, p = 0.031), and the correlation coefficient between humidifying effect and Vpd L was 0.352, also proving impossible to establish a functional model (R 2 = 0.093, p = 0.015). However, the vapor pressure deficit (Vpd L ) of LP and GP had built a linear relationship model with the cooling effect and humidifying effect respectively ( Figure S3c,f). In general, the influence of configuration mode on the correlation between microclimate effects of urban tree species and Vpd L was less than that between the above transpiration characteristic and microclimate effects.

Correlation between Microclimate Regulation and Influencing Factors of ET and DT
The correlation coefficients between the cooling effects of evergreen and deciduous trees and their E were 0.892 and 0.854, and the correlation coefficients of humidifying effects and their E were 0.908 and 0.884, respectively, which were extremely significant (p < 0.01; Table 4). The microclimate effects of ET and DT were fitted with E as a linear model ( Figure S4a,d). Moreover, the fitting coefficient of the linear model of DT was higher than that of ET. Correlation coefficients between the cooling effects of ET and DT and their G s were 0.770 and 0.757, and the correlation coefficients between the humidifying effect and their G s were 0.819 and 0.753, respectively ( Table 4). The linear model of ET had a higher fitting coefficient than DT ( Figure S4b,e).
The microclimate effects of ET and DT were weakly correlated with Vpd L ( Table 4). The function models of ET and DT fitting with Vpd L were both linear models ( Figure S4c,f).
As shown in Table 4, the correlation coefficient between the microclimate effects of ET and DT and 3DGQ were very significant (p < 0.01). We found that when 3DGQ < 250 m 3 and 3DGQ > 650 m 3 , the microclimate regulation capability of DT were most robust than that of ET, while when the 250 m 3 < 3DGQ < 650 m 3 , the microclimate regulation ability of ET were more potent than that of DT (Figure 9a,b).

Correlation between Microclimate Regulation and Influencing Factors of ET and DT
The correlation coefficients between the cooling effects of evergreen and deciduous trees and their E were 0.892 and 0.854, and the correlation coefficients of humidifying effects and their E were 0.908 and 0.884, respectively, which were extremely significant (p < 0.01; Table 4). The microclimate effects of ET and DT were fitted with E as a linear model ( Figure S4a, d). Moreover, the fitting coefficient of the linear model of DT was higher than that of ET.
Correlation coefficients between the cooling effects of ET and DT and their Gs were 0.770 and 0.757, and the correlation coefficients between the humidifying effect and their Gs were 0.819 and 0.753, respectively ( Table 4). The linear model of ET had a higher fitting coefficient than DT ( Figure S4b, e).
The microclimate effects of ET and DT were weakly correlated with VpdL ( Table 4). The function models of ET and DT fitting with VpdL were both linear models ( Figure S4c, f).
As shown in Table 4, the correlation coefficient between the microclimate effects of ET and DT and 3DGQ were very significant (p < 0.01). We found that when 3DGQ < 250 m 3 and 3DGQ > 650 m 3 , the microclimate regulation capability of DT were most robust than that of ET, while when the 250 m 3 < 3DGQ < 650 m 3 , the microclimate regulation ability of ET were more potent than that of DT (Figure 9a, b).

Structural Equation Model of Microclimate Regulation Ability of Urban Trees
In order to explore the influencing factors that affect the microclimate regulation capacity of urban trees, a structural equation model (SEM) was constructed ( Figure 10). The results showed that E, Gs, VpdL, and 3DGQ were critical parameters to measure the microclimate regulation capacity of

Structural Equation Model of Microclimate Regulation Ability of Urban Trees
In order to explore the influencing factors that affect the microclimate regulation capacity of urban trees, a structural equation model (SEM) was constructed ( Figure 10). The results showed that E, G s , Vpd L , and 3DGQ were critical parameters to measure the microclimate regulation capacity of urban trees. Among them, E was significantly positively correlated with G s and 3DGQ, while E was weakly correlated with Vpd L and Vpd L was weakly correlated with 3DGQ. It was surprising that G s did not correlate with VPD, as it did in most studies ( Figure 10). The cooling and humidifying effects of E on urban trees displayed 52% and 41% variation respectively. The cooling and humidifying effects of Gs on trees accounted for 12% and 16% of the variation, respectively; Vpd L could not explain the cooling effect but could explain 13% variation of humidification effect. The cooling and humidifying effects of 3DGQ on trees could explain 38% and 36% of the variation, respectively. Figure 10 indicates that E, G s , and 3DGQ can explain 93% variation in the cooling effect of trees, while E, G s , Vpd L , and 3DGQ can explain 85% variation in the humidifying effect of trees. This also indicates that the microclimate regulation ability of urban trees is the result of the comprehensive action of multiple index parameters. of E on urban trees displayed 52% and 41% variation respectively. The cooling and humidifying effects of Gs on trees accounted for 12% and 16% of the variation, respectively; VpdL could not explain the cooling effect but could explain 13% variation of humidification effect. The cooling and humidifying effects of 3DGQ on trees could explain 38% and 36% of the variation, respectively. Figure 10 indicates that E, Gs, and 3DGQ can explain 93% variation in the cooling effect of trees, while E, Gs, VpdL, and 3DGQ can explain 85% variation in the humidifying effect of trees. This also indicates that the microclimate regulation ability of urban trees is the result of the comprehensive action of multiple index parameters.

Differences of Microclimate Regulation Ability of Urban Trees with Different Tree Species Types and Configuration Modes
Urban trees can improve urban microclimate by reducing summer temperature [33]. Different planting and arrangement of trees may have diverse effects on air temperature modification [6,34]. We conducted a study on the regulating microclimate effect of 10 urban tree species of three configuration modes in Xi'an. Our results revealed that among the three planting configurations of the same tree species, GP showed superior capability in regulating microclimate, and the maximum temperature difference between GP and the control site could reach 5.32 °C. The canopy overlap of trees might explained this planted adjacent to each other increases the cooling and humidifying effects [35]. Similarly, previous researchers also found that the air temperature under solitary tree planting decreased by 1 °C [21] and 1.6 °C [36]. The grove (a small group of trees) has positive effects on the thermal and air quality component of the urban climate [37][38][39]. This indicates that the more complex the configuration mode is, the larger the three-dimensional green quantity of the samples will be, and the stronger microclimate

Differences of Microclimate Regulation Ability of Urban Trees with Different Tree Species Types and Configuration Modes
Urban trees can improve urban microclimate by reducing summer temperature [33]. Different planting and arrangement of trees may have diverse effects on air temperature modification [6,34]. We conducted a study on the regulating microclimate effect of 10 urban tree species of three configuration modes in Xi'an. Our results revealed that among the three planting configurations of the same tree species, GP showed superior capability in regulating microclimate, and the maximum temperature difference between GP and the control site could reach 5.32 • C. The canopy overlap of trees might explained this planted adjacent to each other increases the cooling and humidifying effects [35].
Similarly, previous researchers also found that the air temperature under solitary tree planting decreased by 1 • C [21] and 1.6 • C [36]. The grove (a small group of trees) has positive effects on the thermal and air quality component of the urban climate [37][38][39]. This indicates that the more complex the configuration mode is, the larger the three-dimensional green quantity of the samples will be, and the stronger microclimate regulation capability will be, which is consistent with the findings of Hamada [40] in discussing the relationship between urban air temperature and vegetation coverage. GP has high vegetation coverage rate and large amount of greening, indicating higher microclimate regulation ability. This could explain why configuration modes of trees have different effects on intercepting short-wave radiation and reducing sensible heat [41]. This finding also implies that LP had higher microclimate regulation ability compared to IP. However, it is inconsistent with the findings of Wang and others [38], a possible explanation for this might be that the underlying surface of the samples selected in this study is grassland, while their underlying surface was hard scape, which can offset part of the cooling effect [38], and the transpiration of the trees growing in turf surface are ten times higher than that in the asphalt pavement [42]. Moreover, not just the trees, the underlying grass also transpires. The combination of trees and underlying grass increases total transpiration and thus cooling. Compared with open grassland, the surface temperature of grass under the canopy reduced by 8 • C, indicating the importance of the shading provided by tree crowns [43]. Hence, in order to determine the influence of different configuration methods on the microclimate effect of urban trees, the surface temperatures would be a valuable addition to future research analyzing urban tree effects on the microclimate. When possible, the planting design in this district should focus on increasing the proportion of GP in tree plantings.
Different tree species have unique microclimate regulation potentials [44]. Zhao and his colleagues [34] demonstrated that two foliar longevity species (evergreen and deciduous) might be important in microclimate regulation. Moreover, deciduous tree species had a greater ability to reduce air temperature than evergreen tree species [45]. Rahman et al. [42] and Bowden and Bauerle [46] have observed that differences in leaf and crown morphology may result in species differences in urban microclimatic modification. It is clear from our data that there are significant differences between evergreen and deciduous species and between different planting configurations. In addition, crown volume is a critical driver of increased temperature and humidity modification.

Differences in Transpiration Characteristics of Urban Trees with Different Tree Types and Configuration Modes
We concluded that urban trees in the three configuration modes had different E and G s , which is consistent with the findings of Rahman [47]. This might be due to the interaction and superposition between the canopy of the group planting and the canopy of the trees blocks the direct sunlight penetration, so that the stomata of the leaves do not closed due to excessive-high temperature, and the transpiration rate and stomatal conductance value are not affected. Previous studies have focused on describing the diurnal transpiration of a single tree species, but there has been little quantitative research on the transpiration of urban trees based on different configuration modes of experimental data. Thus, the above conclusions will contribute to a better understanding of the physiological processes of trees.
For the diurnal variation in the transpiration of evergreen and deciduous trees with the three configurations, the transpiration reached a peak at 12 h and declined after 12 h because of the high air temperature at noon, and plants temporarily closed or reduced the stomatal opening and closing degree in order to preserve water, thus vegetation transpiration reached the peak [47]. Meanwhile, as the temperature continues to rise, intense solar radiation will affect the air relative humidity, and water stress may lead to a decrease in the transpiration rate of trees [24]. Therefore, if the trees were irrigated properly, their transpiration rate will rise, which could reduce the heat load of the city. However, transpiration rates of test trees were different from [48], the explanation for this inconsistency might be the specific climate and soil conditions, making it challenging to compare transpiration rates around the world. The effects of urban trees on microclimate through transpiration are highly heterogeneous, for instance, T. cordata has a transpiration rate three times that of R. pseudoacacia [49]. Surprisingly, E and G s of IP were not significantly different, while the differences between LP and GP were significant. This difference may be due to leaf thickness is inversely proportional to transpiration rate in different tree types and configuration modes [42]. Meanwhile, E is greatly influenced by plant characteristics [50]. Based on the above results, the correct selection of trees with excellent transpiration characteristics plays a critical role in reducing the urban heat island effect.
Different tree type is a major factor affecting the total transpiration of tree species in midsummer [51]. The average daily transpiration (E L ) of deciduous trees was higher than that of evergreen trees. Evergreen tree leaves are thick and waxy, which reduce stomatal openness and enhance stomatal resistance so that it helps to reduce the transpiration rate and reduce the transpiration water consumption, such physiological characteristics are conducive to avoid excessive moisture loss [52]. Meanwhile, GP had the highest E L , followed by LP, and IP. Consequently, the transpiration characteristics of urban trees will be affected by the configuration mode and tree types.

Relationship between Microclimate Effect and Transpiration of Trees
Transpiration of urban trees plays a vital role in regulating the canopy thermal environment [53]. In our research, we emphasized the importance of tree transpiration, and the microclimate effects of different tree configurations showed a significant correlation with E and G S . The higher the transpiration rate was, the colder and wetter the surrounding will be, which is consistent with Lindén [54]. Tree species with high transpiration intensity had more heat consumption, and the cooling effect on the surrounding air was obviously better than the area without vegetation [42]. In addition, the transpiration rate not only determines the amount of water vapor evaporated by plants, but also determines the microclimate regulation capacity of plants, which indicate that the transpiration cooling effect of tree species leads to the difference in temperature and humidity in configuration modes. Configurations, and tree species with intense transpiration should be selected to reduce the urban heat island effect, which means more latent heat can be emitted [55].
The increase of air temperature and Vpd L will affect the transpiration of vegetation [56]. We uncovered that the microclimate effect of urban trees with different configurations was weakly correlated with Vpd L , indicating that Vpd L could not explain the cooling effect of experimental trees, which seems to be consistent with the results of Zhou [28]. In hot summer regions, vegetation is vulnerable to temperature and water stress [19], the increase in the air temperature leads to the stomatal opening but the decrease of relative humidity normally leads to stomatal closure, thus enhancing transpiration. However, excessive heat and increased Vpd L between leaves and air would significantly lead to reducing transpiration, which is also the self-protection mechanism of plants against water loss [57]. Hence, the microclimate regulation capacity of urban trees may be affected by transpiration. Based on ensuring pretty appearance and reducing urban heat load, choose tree species with excellent transpiration characteristics for planting design.

Relationship between Microclimate Regulation Ability and Tree Structure Factors
Our results suggest that transpiration is not the only factor affecting the microclimate regulation capacity of urban trees but also depends on the structural factors of trees such as 3DGQ. The current study shows that there is a strong correlation between microclimate regulation capability of trees and 3DGQ. These results support evidence from previous observations [47], which suggested to select species with a high transpiration rate and high value of 3DGQ and planted in clusters designed to improve residents' thermal experience. In our study, the same trees can exert more significant cooling and humidifying potential under the planting mode of GP, which is consistent with the findings of Morakinyo [58]. With the increase of 3DGQ, their canopy coverage rate also increases, and the microclimate regulation ability of trees also becomes more robust [58]. Therefore, 3DGQ could be an index to evaluate the microclimate effects of urban trees.

Limitations and Future Research
Several constraints of this study should be acknowledged. Firstly, this experiment was conducted in the humid subtropical region. The physiological parameters of trees may be different in different cities, and the results cannot be applied to cities with other climatic characteristics. Secondly, this research focuses on the hot summer, which allows long-term monitoring of physiological processes and microclimate effects of urban trees. Thirdly, data were also restricted to sunny days with low wind speeds. Nevertheless, this study provides a reference for designers and managers of campus green space and green park space in the future plant planting design. Landscape architects use the arrangement of group planting with combine shrubs or herbs under the trees, which is beneficial to maximize the cold island effect of urban green space. At the same time, the application proportion of deciduous broadleaf trees should be appropriately increased on both sides of the road or in recreational places to give full play to its cooling and humidifying potential.

Conclusions
In this study, 10 different species of urban trees with three configuration modes were selected. By monitoring the physiological and meteorological parameters of trees, the differences of physiological and meteorological parameters of urban trees with three configuration modes were revealed, as well as the influence of physiological parameters and 3DGQ on the microclimate regulation ability of trees. The principal conclusions could be drawn as follows: (1) Urban trees with three different configuration modes all have specific improving effects on the surrounding environment, among which GP had the most robust microclimate regulation capability, followed by LP and IP. (2) DT had better microclimate regulation ability than ET. The microclimate regulation capability of deciduous trees in GP was always more robust than that of LP and IP. (3) Transpiration characteristics of trees were affected by configuration modes. GP had the best transpiration characteristics, followed by LP and IP. (4) The microclimate regulation capabilities of urban tree species with different configuration modes were affected by E, G s , and 3DGQ, and have a weak relationship with Vpd L . (5) The physiological parameters and 3DGQ of urban trees could explain 93% of the cooling effect and 85% of the humidifying effect. The transpiration characteristics and microclimate effects of urban trees depended primarily on the species and configuration mode of the tree. The microclimate regulation capability of urban trees is the result of multiple parameters.
To sum up, urban trees can significantly improve the urban climate and mitigate the effects of climate change. The arrangement of trees affects their physiological parameters and microclimate regulation, and the cooling and humidifying effects depend more on tree species type and planting design. The results are helpful in selecting a suitable planting layout and tree species to reduce the urban heat island effect. Moreover, the relationship between leaf function traits (leaf thickness, leaf area, leaf anatomical structure) of urban trees and their microclimate regulation ability can be considered in the future studies. Finally, given the above research, more theoretical support can be provided for landscape designers in future planting design to reduce the negative impact of the urban heat island effect.
Supplementary Materials: The following are available online at http://www.mdpi.com/1999-4907/11/8/825/s1, Table S1: Average cooling effect of the three configuration modes. IP: individual planting; LP: linear planting; GP: group planting. Table S2: Average humidifying effect of the three configuration modes. IP: individual planting; LP: linear planting; GP: group planting. Figure S1: Differences in transpiration characteristics among the three configuration modes. (a) E; (b) G s . Significant differences between configuration modes are indicated by different letters (p < 0.05). E: transpiration rate; G s : stomatal conductance. The filled area represents data distribution. Figure S2: Transpiration characteristics of evergreen and deciduous tree species. (a) E; (b) G s . ns: no significant; *represents a significant level (p < 0.05). E: transpiration rate; G s : stomatal conductance; ET: evergreen tree species; DT: deciduous tree species. Figure